Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA最新文献

Objective: To investigate the expressions of lysophosphatidic acid (LPA) receptors at both mRNA and protein levels and their biological significance in different human ovarian cancer cell lines.

Methods: Reverse transcription-polymerase chain reaction (RT-PCR), streptovidin peroxidase-conjugated (SP) immunohistochemical assay and Western blotting were employed to measure the expression levels of LPA(1), LPA(2) and LPA(3) mRNA and LPA(2) and LPA(3) protein in cultured human ovarian cancer cell lines 3AO.SKOV3, and OVCAR3.

Results: All the ovarian cancer cell lines expressed LPA(1), LPA(2) and LPA(3) mRNAs and LPA(2) and LPA(3) proteins.

Conclusion: LPA(1), LPA(2) and LPA(3) expressions occur extensively in human epithelial ovarian cancer cell lines, and LPA(2) and LPA(3) may be involved in the development and progression of human ovarian cancer.

Objective: To evaluate the feasibility and safety of coronary artery angiography via femoral artery approach without heparin.

Methods: Totally 1 400 consecutive patients undergoing coronary artery angiography with heparin were analyzed retrospectively in comparison with the data of 354 patients for coronary artery angiography without heparin.

Results: Success in selective coronary artery angiography was achieved in 99.3% of the patients in the non-heparin group, with the mean operation time of 17.9+/-11.3 min ranging from 8 min to 1 hour. Angiography identified coronary artery stenosis in 72.2% of the patients including 49.8% with multivessel involvement. Subcutaneous hematoma occurred in 25 (1.8%) of the patients, and 1 (0.07%) patient developed arterial and venous fistula and pseudoaneurysm at the puncture site, but other complications (retroperitoneal hematoma, acute myocardial infarction, stroke and peripheral artery thrombotic events) occurred neither during nor after the procedure. In the heparin group, 18 (5.1%) patients developed subcutaneous hematoma and 1 (0.2%) had arterial and venous fistula and pseudoaneurysm at the puncture site after angiography.

Conclusion: Coronary artery angiography without heparin is both safe and feasible with only very low risk of complications.

Objective: To analyze the correlation between serum calcium level and tumor size/ stage in patients with renal cell carcinoma (RCC).

Methods: Totally 173 RCC cases were divided into 3 groups according to their serum calcium level, namely low, normal and high level groups. SPSS10.0 software was used for statistical analysis of the tumor sizes/stages and their association with serum calcium level in the 3 groups.

Results and conclusion: Kruskal-Wallis H test revealed no significant difference in the tumor size between the 3 groups (X(2)=4.768, df=2, P=0.092), but Spearman correlation analysis suggested inverse correlation between serum calcium and tumor size (r=-0.166, P=0.029). Kruskal-Wallis H test also failed to suggest significant difference in the tumor stage between the 3 groups (X(2)=4.277, df=2, P=0.118), but serum calcium was found to be inversely correlated with the tumor stage (r=-0.157, P=0.039 by Spearman correlation analysis).

Objective: To elucidate the significance of the coordination amino acid residues in bilirubin oxidase (BO) and their kinetic characteristics, and evaluate whether BO mutants may serve as better diagnostic agent for hyperbilirubinemia.

Methods: The BO mutants I402G and C457S were obtained by site-directed mutagenesis and confirmed by amino acid sequence analysis. Ru-incorporated C457S mutant was obtained by direct incubation of ruthenium compounds with the mutant. The electron paramagnetic resonance (EPR) spectra of the recombinant BO and the mutants were investigated, and the enzyme kinetics of the recombinant BO and I402G mutant were measured with bilirubin as the substrate at 25 degrees C.

Results: The BO mutants were expressed and purified successfully. The mutant I402G showed low enzyme activity, and had C457S virtually no enzyme activity. Nevertheless Ru-incorporation conferred higher enzyme activity to C457S mutant. The enzyme kinetic investigations revealed that the kinetic parameter k(cat) of the recombinant BO and I402G mutant was 235.8 min(-1) and 6.9 min(-1), respectively, suggesting higher enzyme activity of the recombinant BO.

Conclusions: The coordinating amino acids have important significance in maintaining the integrity of active centers and enzyme activities of recombinant BO and its mutants. The enzyme activities of the mutants I402G and C457S are much lower than those of recombinant BO, therefore they are not appropriate for diagnostic purpose. Ru-incorporation facilitates the formation of a new intact active center in C457S mutant, which therefore acquires enzyme activity.

Objective: To explore the role of complement C3 in delayed-type hypersensitivity (DTH).

Methods: After inducing DTH reaction in the footpads of C3 knockout C3(-/-) and wild-type (C3(+/+) ) mice with ovalbumin (OVA), the thickness of the footpad was measured and HE and immunohistochemical staining preformed to identify the number and types of the infiltrating mononuclear cells in the footpad tissues. T lymphocytes were separated from the spleens of the mice and incubated in 96-well plates with serial dilutions of OVA or mitogens in the presence of mitomycin C-treated macrophages, and the proliferation of the T cells was assessed by (3)H-TdR incorporation assay.

Results: The footpad thickness of DTH-C3(-/-) mice was significantly smaller than that of DTH-C3(+/+) mice. The number of the infiltrating mononuclear cells in the footpad tissue of C3(-/-) mice was obviously decreased in comparison with that of C3(+/+) mice, and the cells were characterized mainly as CD4(+) T lymphocytes. No significant difference in the proliferation of mitogen-stimulated splenic T cells was noted between C3(-/-) and C3(+/+) mice, but after stimulation with the specific antigen OVA, significant reduction in the proliferation of splenic T cells from C3(-/-) mice was observed as compared with the T cell proliferation in C3(+/+) mice.

Conclusion: C3 defect results in impaired DTH responses in mice, which indicates the important role of C3 in DTH reaction.

Objective: To explore the diagnosis and treatment of urinary obstruction involving the transplanted kidney.

Methods: A retrospective analysis was performed in 16 cases of urinary obstruction involving the transplanted kidney, including 5 cases of ureteral calculi, 6 vesicoureteral anastomotic stricture, 2 pyeloureteral junction stricture after transplantation, 1 ureter necrosis due to graft rejection, and 2 infection surrounding the renal graft and ureter end necrosis.

Results: Only one patient had the renal graft removed due to massive hemorrhage in an open surgery for correction of urinary obstruction, and the renal function of the graft was preserved in all the other cases after endoscopic or open surgeries. In the follow-up for 0.5 to 3 years after the second surgery, serum creatinine of the patients were maintained within the range of 90-150 micromol/L, without further renal enlargement or exacerbation of renal retention shown by B-mode ultrasonography.

Conclusion: Urinary obstruction after renal transplantation is a difficult surgical complication, which can be managed by endoscopic or open surgeries depending on the causes of the obstruction.

Objective: To construct a new tissue-engineered bone with poly (D, L-lactide-co-glycolide) (PLGA), bone morphogenetic protein (BMP) and bone marrow-derived stem cells (BMSCs) and observe its effect in repairing segmental bone defects.

Methods: A 15-mm bone defect in the right radius was induced in New Zealand white rabbits, and the models were randomized into three groups to receive implantation of the tissue-engineered bone grafts constructed with PLGA carrying 5 mg BMP and about 1 x 10(6) BMSCs (experimental group), grafts of PLGA with about 1 x 10(6) BMSCs (control group), or grafts of exclusive PLGA (blank control group), respectively. The osteogenesis in the bone defect after the implantation on was evaluated X-ray films, and the histological changes of the tissues sampled from the bone defect 4, 8, and 12 weeks after operation were observed and new bone formation was measured by image analysis.

Results: The bone defect was completely repaired in the experimental group 12 weeks after the implantation, showing the best results among the 3 groups. The bone defects in the blank control group was filled with only fibrous and connective tissues at 12 weeks.

Conclusion: This tissue-engineered bone constructed with PLGA, BMP and BMSCs possesses good ability in repairing segmental bone defect.

Objective: To evaluate the therapeutic effect of of whole brain irradiation (WBI) in the treatment of brain metastases of non-small-cell lung carcinoma and analyze the factors affecting the patients' survival.

Methods: Ninety-three cases of brain metastases of non-small-cell lung carcinoma receiving radiotherapy between January 1998 and February 2004 were retrospectively reviewed. Of these patients, 68 were treated with three-dimensional conformal hypofractionated radiotherapy (3D-CRT) following WBI, while the other 25 underwent 3D-CRT alone. Kaplan-Meier method was used to analyze the survival rate and local control rate, and Cox proportional hazards model employed for determining prognostic factors influencing the patients' survival.

Results: The overall median actuarial survival of the patients was 14 months in the 3D-CRT+WBI group with 1- and 2-year actuarial survival rates of 50% and 27%, respectively, showing no significant difference from 3D-CRT group, which had a median survival of 11 months and 1- and 2-year survival rates of 45% and 15% (P=0.502, log-rank test). Actuarial 1-year local control rate in 3D-CRT+WBI group was 90% as compared to 70% in 3D-CRT group (P=0.028, log-rank test). In multivariate analyses, active extracranial disease (P=0.002) and Karnofsky Performance Scale score (P=0.034) were identified as the independent prognostic factors for the patients' survival.

Conclusion: WBI prior to 3D-CRT does not benefit the patients with brain metastases of non-small cell lung carcinoma for their survival, but may help improve the local control rate.

Objective: To evaluate the effect of double-filtration plasmapheresis (DFPP) in major ABO-incompatible unrelated allogenic bone marrow transplantation.

Methods: The red cell antibodies were removed by DFPP in 5 recipients to prevent hemolysis and pure red cell aplasia following major ABO-incompatible unrelated bone marrow transplantation.

Results: The isohemagglutinin titers of the 5 recipients were below 1:16 after DFPP with only one recipient developed transient mild acute hemolysis. Delayed hemolysis and pure red cell aplasia occurred in none of the cases and all recipients were completely engrafted.

Conclusion: DFPP can effectively prevent hemolysis and pure red cell aplasia in recipients of unrelated bone marrow transplantation with major ABO-incompatibility.

Objective: To prepare forsythiaside from Forsythia suspensa by high-performance liquid chromatography (HPLC).

Method: A crude extract of Forsythia suspensa was prepared by water extraction and alcohol precipitation prior to polyamide column chromatography. Forsythiaside was prepared from the crude extract by RP-HPLC.

Results: Analysis with HPLC showed that the purity of the prepared forsythiaside reached 98%.

Conclusion: HPLC is simple and effective for the preparation of forsythiaside from Forsythia suspensa.