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Two Pathotypes of Xanthomonas oryzae pv. Oryzae Virulence Identified in West Africa 米黄单胞菌的两种致病型。西非稻瘟菌毒力鉴定
Pub Date : 2009-02-01 DOI: 10.3923/crb.2009.22.35
A. Onasanya, M. Ekperigin, F. Nwilene, Y. Séré, R. Onasanya
1 Department of Chemical Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria. 2 Federal University of Technology, Akure, PMB 704, Akure, Ondo State, Nigeria. 3 Institute of Plant Biotechnology for Developing Countries, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium. 4 Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex, PMB 186, Garki, Abuja, Nigeria
1 Babalola大学化学科学系,尼日利亚埃基蒂州阿多埃基蒂。2联邦理工大学,阿库尔,PMB 704,尼日利亚昂多州阿库尔。3根特大学发展中国家植物生物技术研究所,K. L. Ledeganckstraat 35,9000根特,比利时。4生物技术与基因工程高级实验室,Sheda科技综合体,PMB 186,尼日利亚阿布贾加基
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引用次数: 20
Ceftriaxone-sulbactam combination: microbial analysis by variation of ratios and comparative disc diffusion. 头孢曲松-舒巴坦联合用药:用比值变化法和比较圆盘扩散法进行微生物分析。
Pub Date : 2009-02-01 DOI: 10.3923/CRB.2009.50.55
S. Shrivastava, Shailesh Kumar, M. Chaudhary
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引用次数: 9
Antibiotic susceptibility and genetic analysis of Vibrio species isolated from reverine environment. 豚鼠环境分离弧菌的抗生素敏感性及遗传分析。
Pub Date : 2009-02-01 DOI: 10.3923/CRB.2009.36.49
A. Sharma, C. Bora, R. Chaurasia, V. Sahu
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引用次数: 9
First Report of Antimicrobial Spectra of Novel Strain of Streptomyces tritolerans (Strain AS1) Isolated from Earthworm Gut (Eisenia foetida) Against Plant Pathogenic Bacteria and Fungi 从蚯蚓肠道分离的新型三三角链霉菌AS1对植物病原菌和真菌的抑菌光谱研究
Pub Date : 2008-02-01 DOI: 10.3923/CRB.2008.46.55
S. Aruna, K. Vijayalakshmi, M. Shashikanth, M. Rani, K. Jyothi
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引用次数: 8
Study on the Effect of Neem (Azadirachta indica) Leaves Smoke in Controlling Airborne Bacteria in Residential Premises 印楝叶烟对住宅室内空气细菌控制效果的研究
Pub Date : 2008-02-01 DOI: 10.3923/CRB.2008.64.66
S. Khan, J. Aslam
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引用次数: 28
Molecular and Bacteriological Examination of Milk from Different Milch Animals with Special Reference to Coliforms 不同哺乳动物乳中大肠菌群的分子和细菌学检查
Pub Date : 2008-02-01 DOI: 10.3923/CRB.2008.56.63
T. Parekh, R. Subhash
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引用次数: 74
Novel Approaches for Identification of Streptomyces noboritoensis TBG-V20 with Cellulase Production 纤维素酶法鉴定诺硼托链霉菌TBG-V20的新方法
Pub Date : 1900-01-01 DOI: 10.3923/CRB.2010.15.26
R. Arunachalam, E. Wesely, J. George, G. Annadurai
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引用次数: 40
Identification Bacterial Agent of the Bark Canker of Peach in the Province of Ilam 伊拉姆省桃子树皮溃疡病病原菌鉴定
Pub Date : 1900-01-01 DOI: 10.3923/CRB.2013.1.4
M. Gholami
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引用次数: 1
Detection of Esp Enterococcal Surface Protein and Bacteroides Markers in Water Samples 水样中Esp肠球菌表面蛋白和拟杆菌标记物的检测
Pub Date : 1900-01-01 DOI: 10.3923/CRB.2015.11.17
Mohsen Enayati, M. Oshaghi, A. Ahmadi, M. Nahaei, M. Aghazadeh, M. Pourshafie, M. Talebi
Finding the source of fecal pollution in various water resources has been becoming globally important. Molecular marker detection is the most reliable method for bacterial tracking. Since, rapidness is an important factor for the detection of water contamination, we have assessed the detection of water fecal contamination within a short period of time. In this study, twenty-two water samples, including 12 surface water from two rivers in East and West of Tehran (Iran) and 10 water samples from private wells were collected. In addition, five samples were collected from a Sewage Treatment Plant (STP) located in the West of Tehran. All of the samples were tested for the presence of fecal coliforms by Most Probable Number (MPN) method. Polymerase Chain Reaction (PCR) was performed for detection of enterococcal surface protein (Esp) and Bacteroides 16S rRNA. Direct extraction and colony extraction were simultaneously done for the detection of enterococcal contamination. The results showed that the coliform concentration for water samples ranged from >3->1100 MPN/100 mL. The PCR for esp was positive for 5 (23%) water samples. No samples were positive for 16S rRNA for Bacteroides with the exception of the samples obtained from the STP. Our results indicated that using PCR method directly following optimizing DNA extraction from environmental water sources is not suitable for the detection of human enterococcal contamination, while the use of colony extraction method is preferable.
在各种水资源中寻找粪便污染源已成为全球性的重要问题。分子标记检测是最可靠的细菌跟踪方法。由于快速是检测水污染的一个重要因素,因此我们对在短时间内检测水粪便污染进行了评估。本研究收集了22个水样,其中包括12个来自伊朗德黑兰东部和西部两条河流的地表水和10个来自私人水井的水样。此外,从位于德黑兰西部的污水处理厂(STP)收集了5个样本。采用最可能数(MPN)法对所有样本进行粪便大肠菌群检测。采用聚合酶链式反应(PCR)检测肠球菌表面蛋白(Esp)和拟杆菌16S rRNA。采用直接提取法和菌落提取法同时检测肠球菌污染。结果表明,水样中大肠菌群浓度范围为>3 ~ >1100 MPN/100 mL, 5份(23%)水样中esp PCR阳性。除STP样品外,未见拟杆菌16S rRNA阳性。结果表明,优化环境水源DNA提取后直接采用PCR法检测人肠球菌污染不适合,采用菌落提取法检测更为可取。
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引用次数: 0
Isolation and Identification of Lactic Acid Bacteria Isolated from Traditional Drinking Yoghurt in Khartoum State, Sudan 苏丹喀土穆州传统饮用酸奶中乳酸菌的分离与鉴定
Pub Date : 1900-01-01 DOI: 10.3923/CRB.2011.16.22
A. Ali
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引用次数: 33
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Current Research in Bacteriology
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