Rajkishore, G. Banerjee, Soumya Das, R. Tiwari, Ravi Kumar
In this present investigation, two Multi Antibiotics Resistant (MAR) bacterial strains namely SR2 and SR4 were isolated from clinical waste. The bacterial isolate SR2 was resistant to most of the antibiotics tested, but sensitive to levofloxacin. While, the other strain SR4 was sensitive to Cefixime and levofloxacin. Morphological, biochemical and 16S rRNA sequence analysis identified these 2 bacterial strains SR2 and SR4 as Acinetobacter sp. (GenBank Acc. No. KJ879241) and Aeromonas hydrophila (GenBank Acc. No. KJ879242), respectively. The conjugal transfer efficiency of antibiotics resistant gene to another bacterial strain was also tested, using Corynebacterium alkanolyticum ATH3 as a recipient. In order to control these pathogenic bacterial strains, leaf extracts obtained from three Cassia plants named C. siamea, C. allata and C. occidentalis were used at different concentration. Cassia siamea was recorded to be most effective against both of these bacterial strains. Growth kinetics of these two bacterial strains revealed that growth was decreased with gradual increase of concentration of leaf extract, obtained from C. siamea and ultimately negligible growth at 30 mg mLG1 (3%).
本研究从临床废弃物中分离到2株多重抗生素耐药菌株SR2和SR4。分离菌株SR2对大多数抗生素耐药，但对左氧氟沙星敏感。另一株SR4对头孢克肟和左氧氟沙星敏感。形态学、生化和16S rRNA序列分析鉴定这2株菌株SR2和SR4为不动杆菌(Acinetobacter sp.)。不。KJ879241)和嗜水气单胞菌(GenBank Acc)。不。分别KJ879242)。同时，以醇解棒状杆菌ATH3为受体，检测了耐药基因在另一菌株的偶联转移效率。以三种决明子(C. siamea, C. allata, C. occidentalis)叶片提取物为对照，采用不同浓度的提取液对病原菌进行防治。据记录，决明子对这两种细菌菌株都最有效。两株菌株的生长动力学结果表明，随着紫杉叶提取物浓度的逐渐增加，其生长逐渐下降，在30 mg mLG1(3%)浓度下，其生长最终可以忽略不计。
{"title":"Characterization of Drug Resistant Bacteria, Conjugal Transfer Efficiency and their Growth Kinetics Against Cassia Plants Leaf Extract","authors":"Rajkishore, G. Banerjee, Soumya Das, R. Tiwari, Ravi Kumar","doi":"10.3923/CRB.2015.1.10","DOIUrl":"https://doi.org/10.3923/CRB.2015.1.10","url":null,"abstract":"In this present investigation, two Multi Antibiotics Resistant (MAR) bacterial strains namely SR2 and SR4 were isolated from clinical waste. The bacterial isolate SR2 was resistant to most of the antibiotics tested, but sensitive to levofloxacin. While, the other strain SR4 was sensitive to Cefixime and levofloxacin. Morphological, biochemical and 16S rRNA sequence analysis identified these 2 bacterial strains SR2 and SR4 as Acinetobacter sp. (GenBank Acc. No. KJ879241) and Aeromonas hydrophila (GenBank Acc. No. KJ879242), respectively. The conjugal transfer efficiency of antibiotics resistant gene to another bacterial strain was also tested, using Corynebacterium alkanolyticum ATH3 as a recipient. In order to control these pathogenic bacterial strains, leaf extracts obtained from three Cassia plants named C. siamea, C. allata and C. occidentalis were used at different concentration. Cassia siamea was recorded to be most effective against both of these bacterial strains. Growth kinetics of these two bacterial strains revealed that growth was decreased with gradual increase of concentration of leaf extract, obtained from C. siamea and ultimately negligible growth at 30 mg mLG1 (3%).","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133259934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sahu, Priyadharshini Sekar, R. Ramalingam, P. Bhalekar, E. Suguna, G. Palani, P. Krishnan, Menezes Ga
{"title":"Prevalence of Carbapenem Resistant Gram Negative Bacilli Harboring blaNDM-1 Gene Isolated in a Tertiary Care Hospital","authors":"M. Sahu, Priyadharshini Sekar, R. Ramalingam, P. Bhalekar, E. Suguna, G. Palani, P. Krishnan, Menezes Ga","doi":"10.3923/CRB.2016.9.16","DOIUrl":"https://doi.org/10.3923/CRB.2016.9.16","url":null,"abstract":"","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133288088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Mohammed, Cohen Nozha, K. Hakim, Barkia Abdelaziz, M. Houda, F. Abdelaziz, Belahsen Rekia
{"title":"Human Leptospirosis in Morocco 2011","authors":"H. Mohammed, Cohen Nozha, K. Hakim, Barkia Abdelaziz, M. Houda, F. Abdelaziz, Belahsen Rekia","doi":"10.3923/CRB.2012.31.35","DOIUrl":"https://doi.org/10.3923/CRB.2012.31.35","url":null,"abstract":"","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124488212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Sittiwet, S. Jesadanont, P. Pongpech, Penphun Naenna, S. Pongsamart
{"title":"Antibacterial Activity of Malvastrum coromandelianum Garcke Against Methicillin-Sensitive and Methicillin-Resistant Strains of Staphylococcus aureus","authors":"C. Sittiwet, S. Jesadanont, P. Pongpech, Penphun Naenna, S. Pongsamart","doi":"10.3923/CRB.2008.42.45","DOIUrl":"https://doi.org/10.3923/CRB.2008.42.45","url":null,"abstract":"","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123925413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antifungal Activities and Components of VOCs Produced by Bacillus subtilis G8","authors":"Wei-wei Liu, W. Mu, Bing-yu Zhu, Feng Liu","doi":"10.3923/CRB.2008.28.34","DOIUrl":"https://doi.org/10.3923/CRB.2008.28.34","url":null,"abstract":"","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115331727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Free Living Rock Pigeon (Columba livia) as an Environmental Reservoir of Enteric Bacterial Pathogens Resistant to Antimicrobial Drugs in Saudi Arabia","authors":"H. Abulreesh","doi":"10.3923/CRB.2011.28.33","DOIUrl":"https://doi.org/10.3923/CRB.2011.28.33","url":null,"abstract":"","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129671137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarjito, O. Radjasa, A. Sabdono, S. B. Prayitno, S. Hutabarat
A molecular-based study was conducted to estimate the richness of the causative agents of vibriosis associated with groupers from Karimunjawa islands, North Java Sea, Indonesia. Moribound grouper fish were collected from the cage cultures and a total of 32 isolates were isolated from external wound and kidney of groupers. Based on the repetitive sequence-based PCR (rep-PCR) and Koch postulate test, eight isolates were chosen for further sequencings. On the basis of the sequence analysis, the data showed that the causative agents are closely related with Vibrio natriegen, V. o!iviceaus, V fortEs, V alginolitycus, V harveyt, V parahemo!itycus, V damse!a and V carchariae, respectively. Present study highlighted the effectiveness of rep-PCR in rapid grouping and estimating the richness of the causative agents of vibriosis associated with the groupers
{"title":"Phylogenetic Diversity of the Causative Agents of Vibriosis Associated with Groupers Fish from Karimunjawa Islands, Indonesia","authors":"Sarjito, O. Radjasa, A. Sabdono, S. B. Prayitno, S. Hutabarat","doi":"10.3923/CRB.2009.14.21","DOIUrl":"https://doi.org/10.3923/CRB.2009.14.21","url":null,"abstract":"A molecular-based study was conducted to estimate the richness of the causative agents of vibriosis associated with groupers from Karimunjawa islands, North Java Sea, Indonesia. Moribound grouper fish were collected from the cage cultures and a total of 32 isolates were isolated from external wound and kidney of groupers. Based on the repetitive sequence-based PCR (rep-PCR) and Koch postulate test, eight isolates were chosen for further sequencings. On the basis of the sequence analysis, the data showed that the causative agents are closely related with Vibrio natriegen, V. o!iviceaus, V fortEs, V alginolitycus, V harveyt, V parahemo!itycus, V damse!a and V carchariae, respectively. Present study highlighted the effectiveness of rep-PCR in rapid grouping and estimating the richness of the causative agents of vibriosis associated with the groupers","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131805373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. S. Islam, M. Akter, Md. Abiar Rahman, M. M. Rahman, Most Mauluda Akhtar, M. F. Alam
{"title":"Isolation of Agrobacterium tumefaciens strains from crown gall sample of dicot plants in Bangladesh.","authors":"M. S. Islam, M. Akter, Md. Abiar Rahman, M. M. Rahman, Most Mauluda Akhtar, M. F. Alam","doi":"10.3923/CRB.2010.27.36","DOIUrl":"https://doi.org/10.3923/CRB.2010.27.36","url":null,"abstract":"","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130762188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Mukherjee, S. Chaki, S. Barman, Sukhen Das, Hemanta Koley, S. Dastidar
{"title":"Effective Elimination of Drug Resistance Genes in Pathogenic Pseudomonas aeruginosa by Antipsychotic Agent Thioridazine","authors":"S. Mukherjee, S. Chaki, S. Barman, Sukhen Das, Hemanta Koley, S. Dastidar","doi":"10.3923/CRB.2012.36.41","DOIUrl":"https://doi.org/10.3923/CRB.2012.36.41","url":null,"abstract":"","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128665005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Study of Compositions and Antimicrobial Properties of Essential Oil of Origanum vulgare and Rosmarinus officinalis on Human Pathogens","authors":"M. Kazemi, H. Rostami, A. Ameri","doi":"10.3923/CRB.2012.1.12","DOIUrl":"https://doi.org/10.3923/CRB.2012.1.12","url":null,"abstract":"","PeriodicalId":111056,"journal":{"name":"Current Research in Bacteriology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131125618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：