Pub Date : 2023-04-01DOI: 10.4103//DS.V41-1_Editorial
C. Hsu
{"title":"Scoring systems for pemphigus","authors":"C. Hsu","doi":"10.4103//DS.V41-1_Editorial","DOIUrl":"https://doi.org/10.4103//DS.V41-1_Editorial","url":null,"abstract":"","PeriodicalId":11107,"journal":{"name":"Dermatologica Sinica","volume":"89 1","pages":"65 - 66"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79983013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.4103/ds.ds-d-22-00205
Yusuf Kelleci, F. Aydın, Gokhan Sahin, E. Yuksel
Background: Alopecia areata (AA) is a disease of unknown etiology which is thought to be autoimmune, that presents challenges in terms of treatment. Platelet-rich plasma (PRP) can induce the development of new hair follicles through various growth factors it contains. PRP could be a treatment option for patients with AA. Objectives: We aim to evaluate the effectiveness and safety of PRP treatment in patients with patch-type AA. Methods: The study included 17 patients diagnosed with patch-type AA who received PRP treatment at least 3 sessions every 4 weeks. Treatment response was evaluated by calculating the severity of alopecia tool (SALT) scores. Results: The mean age of patients was 29.70 ± 10.09, and the mean disease duration was 18.17 ± 39.46 months. The mean SALT score of 17 patients was 6.82 ± 4.96 and 4.35 ± 4.58 before treatment and at the end of the 3rd month, respectively. The reduction in SALT scores was statistically significant (P = 0.01). At the end of the 3rd month, six patients (35.3%) had an excellent response, one patient (5.9%) had a good response, one patient (5.9%) had a partial response, and nine patients (52.9%) showed no response. The mean SALT scores of four patients who received six sessions of PRP of treatment were 6.00 ± 2.94 and 1.75 ± 2.06 at the end of the 6th month. No serious side effects were noted. Relapse was observed in one patient within 1 year of follow-up. Conclusion: As a result of our study, PRP treatment was found to be an effective and reliable treatment method in patients with mild AA.
{"title":"Platelet-rich plasma in the treatment of alopecia areata: A retrospective evaluation of 17 patients","authors":"Yusuf Kelleci, F. Aydın, Gokhan Sahin, E. Yuksel","doi":"10.4103/ds.ds-d-22-00205","DOIUrl":"https://doi.org/10.4103/ds.ds-d-22-00205","url":null,"abstract":"Background: Alopecia areata (AA) is a disease of unknown etiology which is thought to be autoimmune, that presents challenges in terms of treatment. Platelet-rich plasma (PRP) can induce the development of new hair follicles through various growth factors it contains. PRP could be a treatment option for patients with AA. Objectives: We aim to evaluate the effectiveness and safety of PRP treatment in patients with patch-type AA. Methods: The study included 17 patients diagnosed with patch-type AA who received PRP treatment at least 3 sessions every 4 weeks. Treatment response was evaluated by calculating the severity of alopecia tool (SALT) scores. Results: The mean age of patients was 29.70 ± 10.09, and the mean disease duration was 18.17 ± 39.46 months. The mean SALT score of 17 patients was 6.82 ± 4.96 and 4.35 ± 4.58 before treatment and at the end of the 3rd month, respectively. The reduction in SALT scores was statistically significant (P = 0.01). At the end of the 3rd month, six patients (35.3%) had an excellent response, one patient (5.9%) had a good response, one patient (5.9%) had a partial response, and nine patients (52.9%) showed no response. The mean SALT scores of four patients who received six sessions of PRP of treatment were 6.00 ± 2.94 and 1.75 ± 2.06 at the end of the 6th month. No serious side effects were noted. Relapse was observed in one patient within 1 year of follow-up. Conclusion: As a result of our study, PRP treatment was found to be an effective and reliable treatment method in patients with mild AA.","PeriodicalId":11107,"journal":{"name":"Dermatologica Sinica","volume":"85 1","pages":"111 - 115"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77850899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.4103/ds.ds-d-22-00141
Yuan Zheng, Pingdong Jiang, Liyin Zhang
Background: Malignant melanoma is a typical type of cancer that feature the obesity paradox. Objectives: We aim to evaluate the role of acyl-CoA synthetase medium-chain 3 (ACSM3) in obesity paradox in malignant melanoma (MM). Methods: With reproduction of the Cancer Genome Atlas (TCGA) MM dataset and validation using immunohistochemistry, we evaluated correlations of ACSM3 expression with body mass index (BMI), gender, and androgen receptor (AR) expression. Results: ACSM family genes were downregulated in MM and in normal skin exposed to ultraviolet. Higher expression of ACSM3 was associated with improved overall survival in men but not in women. Subgroup analysis showed the best survival outcome in obese patients with high ACSM3 expression. ACSM3 expression was significantly lower in cases with ulceration. NET-GE-based gene enrichment analysis of ACSM-overexpressed cases showed significant enrichment in lipid metabolism and butanoate metabolism. AR stood as the top possible transcription factor of ACSM3 using multiple algorithms. Expressions of ACSM3 and AR were positively correlated in obese men and overweight women. ACSM3 expression was positively correlated with BMI in men and overweight women. Conclusion: ACSM3 expression is associated with obesity in MM patients and correlates with AR. Functional analysis linking the findings to the obesity paradox warrants further study.
{"title":"Expression of acyl-CoA synthetase medium-chain 3 is associated with obesity in melanoma patients and correlates with androgen receptor","authors":"Yuan Zheng, Pingdong Jiang, Liyin Zhang","doi":"10.4103/ds.ds-d-22-00141","DOIUrl":"https://doi.org/10.4103/ds.ds-d-22-00141","url":null,"abstract":"Background: Malignant melanoma is a typical type of cancer that feature the obesity paradox. Objectives: We aim to evaluate the role of acyl-CoA synthetase medium-chain 3 (ACSM3) in obesity paradox in malignant melanoma (MM). Methods: With reproduction of the Cancer Genome Atlas (TCGA) MM dataset and validation using immunohistochemistry, we evaluated correlations of ACSM3 expression with body mass index (BMI), gender, and androgen receptor (AR) expression. Results: ACSM family genes were downregulated in MM and in normal skin exposed to ultraviolet. Higher expression of ACSM3 was associated with improved overall survival in men but not in women. Subgroup analysis showed the best survival outcome in obese patients with high ACSM3 expression. ACSM3 expression was significantly lower in cases with ulceration. NET-GE-based gene enrichment analysis of ACSM-overexpressed cases showed significant enrichment in lipid metabolism and butanoate metabolism. AR stood as the top possible transcription factor of ACSM3 using multiple algorithms. Expressions of ACSM3 and AR were positively correlated in obese men and overweight women. ACSM3 expression was positively correlated with BMI in men and overweight women. Conclusion: ACSM3 expression is associated with obesity in MM patients and correlates with AR. Functional analysis linking the findings to the obesity paradox warrants further study.","PeriodicalId":11107,"journal":{"name":"Dermatologica Sinica","volume":"23 1","pages":"87 - 93"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76551356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.4103/ds.ds-d-23-00004
Y. Hsu, T. Tsai
{"title":"A case of new-onset palmoplantar pustulosis following mRNA COVID-19 vaccination","authors":"Y. Hsu, T. Tsai","doi":"10.4103/ds.ds-d-23-00004","DOIUrl":"https://doi.org/10.4103/ds.ds-d-23-00004","url":null,"abstract":"","PeriodicalId":11107,"journal":{"name":"Dermatologica Sinica","volume":"58 1","pages":"127 - 128"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85050390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.4103/ds.ds-d-23-00001
W. Nho, Junyoung Kim, S. Kee, Dong-Jin Kim, K. Yoon
{"title":"Giant perforating pilomatricoma: A case report","authors":"W. Nho, Junyoung Kim, S. Kee, Dong-Jin Kim, K. Yoon","doi":"10.4103/ds.ds-d-23-00001","DOIUrl":"https://doi.org/10.4103/ds.ds-d-23-00001","url":null,"abstract":"","PeriodicalId":11107,"journal":{"name":"Dermatologica Sinica","volume":"49 1","pages":"129 - 130"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73605088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.4103/ds.DS-D-22-00103
Shu-Hao Li, C. Chiou, Chien-Chin Chen
Primary cutaneous mucinous carcinoma (PCMC) is rare, and its carcinogenesis is unclear. Trimethylation of histone H3 at lysine 27 (H3K27me3) is a key regulator in chromatin remodeling-controlled transcription. Focusing on the epigenetic mechanism, we aimed to investigate the expression of H3K27me3 in PCMC by immunohistochemistry. A retrospective cohort of PCMC patients from a tertiary hospital in Taiwan was enrolled to evaluate the clinicopathologic features, treatment outcome, and protein expression. Immunohistochemistry for H3K27me3 was performed on all PCMCs and a comparison group of colonic mucinous adenocarcinoma and pure mucinous carcinoma of the breast. The percentage of H3K27me3-negative tumor cells was calculated and analyzed. Three patients with PCMC were recruited. All PCMCs were solitary and slow growing, arising from the head-and-neck region. All PCMCs had tumor excision without local recurrence or metastasis. The loss of H3K27me3 expression was significant in PCMCs (mean ± standard deviation [SD]: 21.0% ± 6.6%) compared to other mucinous carcinomas (mean ± SD: 3.8% ± 1.7%) (P = 0.019). In conclusion, we report a reduction in H3K27me3 expression in PCMC. In contrast, H3K27me3 expression is retained or mildly reduced in other mucinous carcinomas. This is the first study to indicate a possible role of epigenetic events in the pathobiology of PCMC.
{"title":"The reduced expression of trimethylation of histone H3 at lysine 27 in primary cutaneous mucinous carcinoma","authors":"Shu-Hao Li, C. Chiou, Chien-Chin Chen","doi":"10.4103/ds.DS-D-22-00103","DOIUrl":"https://doi.org/10.4103/ds.DS-D-22-00103","url":null,"abstract":"Primary cutaneous mucinous carcinoma (PCMC) is rare, and its carcinogenesis is unclear. Trimethylation of histone H3 at lysine 27 (H3K27me3) is a key regulator in chromatin remodeling-controlled transcription. Focusing on the epigenetic mechanism, we aimed to investigate the expression of H3K27me3 in PCMC by immunohistochemistry. A retrospective cohort of PCMC patients from a tertiary hospital in Taiwan was enrolled to evaluate the clinicopathologic features, treatment outcome, and protein expression. Immunohistochemistry for H3K27me3 was performed on all PCMCs and a comparison group of colonic mucinous adenocarcinoma and pure mucinous carcinoma of the breast. The percentage of H3K27me3-negative tumor cells was calculated and analyzed. Three patients with PCMC were recruited. All PCMCs were solitary and slow growing, arising from the head-and-neck region. All PCMCs had tumor excision without local recurrence or metastasis. The loss of H3K27me3 expression was significant in PCMCs (mean ± standard deviation [SD]: 21.0% ± 6.6%) compared to other mucinous carcinomas (mean ± SD: 3.8% ± 1.7%) (P = 0.019). In conclusion, we report a reduction in H3K27me3 expression in PCMC. In contrast, H3K27me3 expression is retained or mildly reduced in other mucinous carcinomas. This is the first study to indicate a possible role of epigenetic events in the pathobiology of PCMC.","PeriodicalId":11107,"journal":{"name":"Dermatologica Sinica","volume":"84 1","pages":"116 - 120"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88495105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.4103/ds.ds-d-22-00173
Nermin Ozlen, Deniz Ercetin, M. Sapmaz-Metin, O. Gunduz
Background: Hydrogen sulfide (H2S), synthesized by most immune cells, has been shown to exert anti-inflammatory and antipruritic effects. The effect of H2S on allergic contact dermatitis (ACD), an inflammatory skin disease that negatively affects the quality of life, is unknown. Objectives: We planned to investigate the antipruritic and anti-inflammatory effects of the H2S donor sodium sulfide (Na2S) in the experimental mouse model of contact hypersensitivity (CHS), which is widely used for ACD research. Methods: CHS was created in Balb/c mice using 1-fluoro-2, 4-dinitrobenzene. Na2S was administered systemically (0.2-2-20 mg/kg/i.p.) and locally (1-3-10 nmol/both ear/i.d.) at 3 h and 25 h after the challenge. Ear thickness and the number of scratches were determined at 24 h and 48 h following the challenge. Ear tissue and serum interferon-gamma, interleukin (IL)-2, IL-4, and IL-5 cytokine levels were evaluated by enzyme-linked immunosorbent assay (ELISA). H and E staining was performed for histopathological studies. CD4+ and CD8+ T cells located in the skin were examined by immunohistochemical staining. Results: Locally (1-3-10 nmol/ear/i.d., P < 0.001, P < 0.0001, P < 0.0001, respectively) and systemically (2–20 mg/kg/i.p., P < 0.01, P < 0.0001, respectively), Na2S administration decreased ear thickness dose dependently. Local (1-3-10 nmol/ear/i.d.) Na2S treatment decreased serum IL-2 levels (P < 0.01, P < 0.05, and P < 0.01, respectively). Na2S administered locally (3–10 nmol/ear/i.d., P < 0.05) and systemically (20 mg/kg/i. p., P < 0.05) decreased the number of CD4+ T lymphocytes. Conclusion: Locally and systemically administered Na2S reduces ear thickness, which is one of the symptoms of CHS, probably by preventing CD4+ T lymphocyte infiltration and proliferation and decreasing IL-2 synthesis.
背景:硫化氢(H2S)由大多数免疫细胞合成，已被证明具有抗炎和止痒作用。H2S对过敏性接触性皮炎(ACD)的影响尚不清楚，ACD是一种对生活质量产生负面影响的炎症性皮肤病。目的:研究H2S供体硫化钠(Na2S)对ACD研究中广泛使用的接触性超敏反应(CHS)小鼠模型的止痒和抗炎作用。方法:用1-氟- 2,4 -二硝基苯制备Balb/c小鼠CHS。Na2S分别于攻毒后3 h和25 h全身(0.2-2-20 mg/kg/i.p)和局部(1-3-10 nmol/双耳/i.d)给药。在刺激后24 h和48 h测定耳朵厚度和划痕数量。采用酶联免疫吸附试验(ELISA)检测小鼠耳组织及血清中干扰素- γ、白细胞介素(IL)-2、IL-4和IL-5细胞因子水平。H、E染色进行组织病理学研究。免疫组化染色检测皮肤内CD4+和CD8+ T细胞。结果:局部(1-3-10 nmol/耳/次);， P < 0.001, P < 0.0001, P < 0.0001)和全身(2-20 mg/kg/i.p)。(P < 0.01, P < 0.0001)， Na2S对耳厚的影响呈剂量依赖性。局部(1-3-10 nmol/耳/ id)Na2S处理降低了血清IL-2水平(P < 0.01, P < 0.05, P < 0.01)。局部给药Na2S (3 ~ 10 nmol/耳/次)。， P < 0.05)和全身(20 mg/kg/i)。p.， p < 0.05)降低CD4+ T淋巴细胞数量。结论:局部及全身给药Na2S可减少耳厚，这是CHS的症状之一，可能通过阻止CD4+ T淋巴细胞的浸润和增殖，降低IL-2的合成。
{"title":"Anti-inflammatory effect of hydrogen sulfide donor sodium-sulfide in an experimental mouse model of contact hypersensitivity","authors":"Nermin Ozlen, Deniz Ercetin, M. Sapmaz-Metin, O. Gunduz","doi":"10.4103/ds.ds-d-22-00173","DOIUrl":"https://doi.org/10.4103/ds.ds-d-22-00173","url":null,"abstract":"Background: Hydrogen sulfide (H2S), synthesized by most immune cells, has been shown to exert anti-inflammatory and antipruritic effects. The effect of H2S on allergic contact dermatitis (ACD), an inflammatory skin disease that negatively affects the quality of life, is unknown. Objectives: We planned to investigate the antipruritic and anti-inflammatory effects of the H2S donor sodium sulfide (Na2S) in the experimental mouse model of contact hypersensitivity (CHS), which is widely used for ACD research. Methods: CHS was created in Balb/c mice using 1-fluoro-2, 4-dinitrobenzene. Na2S was administered systemically (0.2-2-20 mg/kg/i.p.) and locally (1-3-10 nmol/both ear/i.d.) at 3 h and 25 h after the challenge. Ear thickness and the number of scratches were determined at 24 h and 48 h following the challenge. Ear tissue and serum interferon-gamma, interleukin (IL)-2, IL-4, and IL-5 cytokine levels were evaluated by enzyme-linked immunosorbent assay (ELISA). H and E staining was performed for histopathological studies. CD4+ and CD8+ T cells located in the skin were examined by immunohistochemical staining. Results: Locally (1-3-10 nmol/ear/i.d., P < 0.001, P < 0.0001, P < 0.0001, respectively) and systemically (2–20 mg/kg/i.p., P < 0.01, P < 0.0001, respectively), Na2S administration decreased ear thickness dose dependently. Local (1-3-10 nmol/ear/i.d.) Na2S treatment decreased serum IL-2 levels (P < 0.01, P < 0.05, and P < 0.01, respectively). Na2S administered locally (3–10 nmol/ear/i.d., P < 0.05) and systemically (20 mg/kg/i. p., P < 0.05) decreased the number of CD4+ T lymphocytes. Conclusion: Locally and systemically administered Na2S reduces ear thickness, which is one of the symptoms of CHS, probably by preventing CD4+ T lymphocyte infiltration and proliferation and decreasing IL-2 synthesis.","PeriodicalId":11107,"journal":{"name":"Dermatologica Sinica","volume":"31 1","pages":"94 - 102"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84987251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.4103/ds.DS-D-22-00150
Darby Boucher, Anna L. Wilson, Dedee Murrell
Pemphigus is a rare autoimmune blistering disease that affects the skin and mucous membranes. Despite recent advances in treatment, pemphigus is associated with high patient mortality and has a significantly negative impact on patients' quality of life and mental health. Validated scoring systems to accurately measure pemphigus severity are vital for the monitoring of disease activity, evaluation of treatment efficacy and trialing, comparison and introduction of new treatments. This article reviews the existing scoring systems for pemphigus and their validation studies and proposes a future area of research regarding global assessments for simplistic scoring of pemphigus severity.
{"title":"Pemphigus scoring systems and their validation studies – A review of the literature","authors":"Darby Boucher, Anna L. Wilson, Dedee Murrell","doi":"10.4103/ds.DS-D-22-00150","DOIUrl":"https://doi.org/10.4103/ds.DS-D-22-00150","url":null,"abstract":"Pemphigus is a rare autoimmune blistering disease that affects the skin and mucous membranes. Despite recent advances in treatment, pemphigus is associated with high patient mortality and has a significantly negative impact on patients' quality of life and mental health. Validated scoring systems to accurately measure pemphigus severity are vital for the monitoring of disease activity, evaluation of treatment efficacy and trialing, comparison and introduction of new treatments. This article reviews the existing scoring systems for pemphigus and their validation studies and proposes a future area of research regarding global assessments for simplistic scoring of pemphigus severity.","PeriodicalId":11107,"journal":{"name":"Dermatologica Sinica","volume":"3 1","pages":"67 - 77"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91025734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: We aimed to explore the therapeutic biomarker for cutaneous melanoma (CM). Objectives: The objective is to provide a novel direction for improving overall survival (OS) for CM. Methods: We obtained the gene sets related to ultraviolet (UV) reaction from MsigDB database and CM HTSeq-FPKM data from The Cancer Genome Atlas (TCGA). Gene set variation analysis was used to calculate the enrichment scores in each sample. DAVID and Gene Set Enrichment Analysis (GSEA) were used to explore the function of differentially expressed genes (DEGs) between cluster 1 and cluster 2. The ssGSEA was used to analyze the degree of immune infiltration in samples. Weighted gene co-expression network analysis (WGCNA), protein–protein interaction (PPI) network, and mutation analysis were performed to screen the DEGs related to UV response. Results: The samples were divided into the high activity of UV response (cluster 1) and low activity of UV response (cluster 2). We found that cluster 2 was related to poorer OS and had a higher reaction to UV response. Function analysis indicated that the DEGs are involved in angiogenesis, epidermal development, and inflammatory reaction. Furthermore, the cluster 2 had a higher degree of immune infiltration. The results of WGCNA indicated that the genes in the MEyellow module were highly related to UV response, which is involved in the process of angiogenesis, cell migration, and skin development. PPI and mutation analysis indicated that COL5A1 was the risk factor for CM. Conclusion: COL5A1 might be an important biomarker and potential therapeutic target of CM.
背景:我们旨在探索皮肤黑色素瘤(CM)的治疗性生物标志物。目的:目的是为提高CM的总生存期(OS)提供一个新的方向。方法:从美国癌症基因组图谱(the Cancer Genome Atlas, TCGA)中获取与紫外线(UV)反应相关的基因集和CM HTSeq-FPKM数据。基因集变异分析用于计算每个样品的富集分数。采用DAVID和基因集富集分析(Gene Set Enrichment Analysis, GSEA)分析了集群1和集群2之间差异表达基因(differential expression genes, DEGs)的功能。采用ssGSEA分析样品免疫浸润程度。通过加权基因共表达网络分析(WGCNA)、蛋白-蛋白相互作用网络(PPI)和突变分析来筛选与紫外线反应相关的基因。结果:将样品分为高活性紫外响应(簇1)和低活性紫外响应(簇2)。我们发现簇2与较差的OS相关，对紫外响应的反应较高。功能分析表明，deg参与血管生成、表皮发育和炎症反应。此外，簇2具有较高的免疫浸润程度。WGCNA结果表明，MEyellow模块中的基因与紫外线反应高度相关，参与血管生成、细胞迁移和皮肤发育过程。PPI和突变分析显示COL5A1是CM的危险因素。结论:COL5A1可能是CM重要的生物标志物和潜在的治疗靶点。
{"title":"Transcriptomic profiling and classification of skin melanoma based on ultraviolet response","authors":"Dongxing Xiao, Zhaozhao Guo, Yuzhen Xiong, Xinqiang He, Chong Zhao, N. Tang","doi":"10.4103/ds.ds-d-22-00178","DOIUrl":"https://doi.org/10.4103/ds.ds-d-22-00178","url":null,"abstract":"Background: We aimed to explore the therapeutic biomarker for cutaneous melanoma (CM). Objectives: The objective is to provide a novel direction for improving overall survival (OS) for CM. Methods: We obtained the gene sets related to ultraviolet (UV) reaction from MsigDB database and CM HTSeq-FPKM data from The Cancer Genome Atlas (TCGA). Gene set variation analysis was used to calculate the enrichment scores in each sample. DAVID and Gene Set Enrichment Analysis (GSEA) were used to explore the function of differentially expressed genes (DEGs) between cluster 1 and cluster 2. The ssGSEA was used to analyze the degree of immune infiltration in samples. Weighted gene co-expression network analysis (WGCNA), protein–protein interaction (PPI) network, and mutation analysis were performed to screen the DEGs related to UV response. Results: The samples were divided into the high activity of UV response (cluster 1) and low activity of UV response (cluster 2). We found that cluster 2 was related to poorer OS and had a higher reaction to UV response. Function analysis indicated that the DEGs are involved in angiogenesis, epidermal development, and inflammatory reaction. Furthermore, the cluster 2 had a higher degree of immune infiltration. The results of WGCNA indicated that the genes in the MEyellow module were highly related to UV response, which is involved in the process of angiogenesis, cell migration, and skin development. PPI and mutation analysis indicated that COL5A1 was the risk factor for CM. Conclusion: COL5A1 might be an important biomarker and potential therapeutic target of CM.","PeriodicalId":11107,"journal":{"name":"Dermatologica Sinica","volume":"40 1","pages":"103 - 110"},"PeriodicalIF":2.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85745449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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