Developmental and comparative immunology最新文献

{"title":"Peripheral blood mononuclear cell-driven cytokine and transcription factors induction confers resistance to Haemonchus contortus in Black Bengal goats","authors":"Nurnabi Ahmed ,&nbsp;Babul Chandra Roy ,&nbsp;Amitav Biswas ,&nbsp;Md Rajiur Rahaman Rabbi ,&nbsp;Md Mahfuzur Rahman Sajib ,&nbsp;Mohammad Manjurul Hasan ,&nbsp;Hiranmoy Biswas ,&nbsp;MD Hasanuzzaman Talukder","doi":"10.1016/j.dci.2025.105470","DOIUrl":"10.1016/j.dci.2025.105470","url":null,"abstract":"<div><div><em>Haemonchus contortus</em> is a major threat to small ruminant health and productivity. Although early Th2 cytokine and transcription factor expression confers protection in naturally resistant breeds, the immune basis of resistance in Black Bengal goats (BBG) remains unexplored. We compare early PBMC-mediated cytokine and transcriptional responses and their direct effects on larval motility between naive and primed BBG kids during the first seven days of infection. Kids were primed with 2000 L₃ weekly for four weeks or left naive, then challenged with 10,000 L₃. Two kids per group were sacrificed on each time point. Whole blood was collected pre-mortem for differential counts and PBMC isolation, while abomasal mucosa and draining LN were harvested for histology and RNA extraction. Primed kids exhibited a 58 % reduction in abomasal L₄ burden by day 7, elevated PCV (<em>p &lt;</em> 0.05), and a threefold greater increase in LN weight compared to naive kids. Histopathology revealed significantly enhanced eosinophil and neutrophil infiltration in abomasal mucosa of primed kids. Cytokine and gene expression assay showed early upregulation of Interleukin (IL)-4, IL-5, IL-13, IL-33, MCP-1, CXCL-1, TLR-2, and GAL-14 (p &lt; 0.05). <em>In vitro</em>, co-culture with primed PBMCs reduced L₃ motility compared to naive PBMCs (p &lt; 0.01) and L₃ pretreated with primed PBMCs resulted in a 60 % reduction in fecal egg counts by week 5 (p &lt; 0.001). This is the first study to integrate daily PBMC transcriptomics with functional motility and infectivity assays in BBGs. The findings identify novel biomarkers, inform selective breeding and immunoprophylactic strategies for sustainable parasite control.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105470"},"PeriodicalIF":2.4,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional characterization of complement receptor 3 (CR3) in Nile tilapia (Oreochromis niloticus): Insights into CD11b/CD18-mediated immunity against bacterial infections","authors":"Yang Lei ,&nbsp;Weiheng Shi ,&nbsp;Yanxi Guo ,&nbsp;Yuqing Lin ,&nbsp;Jianmin Ye ,&nbsp;Liting Wu","doi":"10.1016/j.dci.2025.105469","DOIUrl":"10.1016/j.dci.2025.105469","url":null,"abstract":"<div><div>Complement receptor 3 (CR3), also known as integrin αMβ2, CD11b/CD18, or Mac-1, is a heterodimeric leukocyte-specific integrin composed of the αM (CD11b) and β2 (CD18) subunits. To elucidate the role of CR3 in immunity in Nile tilapia (<em>Oreochromis niloticus</em>), we cloned and characterized the αM (<em>On</em>CD11b) and β2 (<em>On</em>CD18) subunits and investigated their functions both <em>in vivo</em> and <em>in vitro</em>. Sequence analysis revealed that <em>On</em>CD11b contains a 3378-bp open reading frame (ORF) encoding a protein of 1128 amino acids (124.7 kDa), while <em>On</em>CD18 comprises a 2337-bp ORF encoding 778 amino acids (85.7 kDa). Structural alignment demonstrated high degree of conservation in the von Willebrand factor type A (vWFA) domains of both subunits, with significant homology to CD11b and CD18 orthologs across species. Phylogenetic analysis confirmed that <em>On</em>CD11b and <em>On</em>CD18 cluster within the teleost-specific CD11b and CD18 clades, respectively. Tissue-specific expression profiling indicated predominant expression of <em>On</em>CD11b in the head kidney and <em>On</em>CD18 in the spleen. Both subunits were significantly upregulated in these tissues following challenges with <em>Streptococcus agalactiae</em> (<em>S. agalactiae</em>) and <em>Aeromonas hydrophila</em> (<em>A. hydrophila</em>), suggesting their involvement in pathogen-induced immune responses. <em>In vitro</em> functional assays demonstrated that the recombinant vWFA domains of <em>On</em>CD11b and <em>On</em>CD18 exhibited specific binding capacity to <em>S. agalactiae</em>, <em>A. hydrophila</em>, and lipopolysaccharide, highlighting their role as pattern recognition receptors. Crucially, <em>in vivo</em> knockdown of <em>On</em>CD11b or <em>On</em>CD18 resulted in a significant increase in bacterial load in tilapia tissues following <em>S. agalactiae</em> infection, underscoring their essential role in host defense. These findings collectively demonstrate that <em>On</em>CD11b and <em>On</em>CD18 are pivotal components of the immune system in Nile tilapia, facilitating bacterial clearance through direct pathogen recognition pathway. This study provides new insights into the evolutionarily conserved mechanisms of CR3-mediated immunity and potential therapeutic targets for bacterial infections in aquaculture species.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105469"},"PeriodicalIF":2.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145098908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional MDA5 knockout in CHSE-214 cells decreases the response to long dsRNA and enhances Chum Salmon Reovirus susceptibility","authors":"Shayne Oberhoffner ,&nbsp;Mathilde Peruzzi ,&nbsp;Bertrand Collet ,&nbsp;Stephanie DeWitte-Orr","doi":"10.1016/j.dci.2025.105466","DOIUrl":"10.1016/j.dci.2025.105466","url":null,"abstract":"<div><div>Many organisms express sensors that detect long dsRNA, a nucleic acid produced by almost all viruses during replication. These subcellular compartment-specific pattern recognition receptors (PRRs) detect viral replication and rapidly induce a generalist immune response mediated by type I interferon and interferon-stimulated genes (ISGs). In this study, the role of melanoma differentiation-associated protein (MDA)5, a cytoplasmic sensor of long dsRNA, was measured in CHSE-214 cells that have been genetically modified for MDA5 to either to be non-functional (frameshift mutation; MDA5C1) or have a silent point mutation (MDA5C2). These cells were transfected with long dsRNA of either 659bp or 334bp in length, and their ability to induce ISG transcript expression (<em>Mx1, vig-3</em> and <em>vig-4</em>) was measured by RT-qPCR. MDA5C1 produced significantly less <em>Mx1, vig-3</em> and <em>vig-4</em> transcripts compared to MDA5C2 when treated with the 659bp dsRNA molecule, while there was no difference between cell lines in <em>Mx1</em> expression levels when induced by a 334 bp dsRNA molecule. MDA5C1 was more susceptible to CSV than MDA5C2, and dsRNA treatment was able to protect both cell lines from CSV infection. However, there were no length dependent effects observed, nor were there differences in dsRNA-mediated protection between cell lines. This data suggests that salmonid MDA5 has a length preference similar to mammals, with longer dsRNA molecules being better ligands for this receptor. It also suggests that MDA5 may play a role in the antiviral response against CSV but the antiviral response is not dependent on MDA5. These are important findings for understanding the fundamental importance of MDA5 in the antiviral response of salmonids.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105466"},"PeriodicalIF":2.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Integrin β1 and its effects on cell adhesion, migration and apoptosis in Sebastes schlegelii","authors":"Xiaohua Yuan ,&nbsp;Xinqing Ma ,&nbsp;Haitao Sun ,&nbsp;Min Zhang ,&nbsp;Jingjing Wang","doi":"10.1016/j.dci.2025.105467","DOIUrl":"10.1016/j.dci.2025.105467","url":null,"abstract":"<div><div>Integrin β1, a transmembrane receptor, plays a pivotal role in regulating cell adhesion, migration, signal transduction, and apoptosis in diverse tissues and cell types. In this study, we identified <em>integrin β1</em> from <em>Sebastes schlegelii</em> (<em>SsITGβ1</em>). The <em>SsITGβ1</em> cDNA spans 4017 bp, including a 332 bp 5′ untranslated region (UTR), a 1285 bp 3′ UTR, and a 2400 bp open reading frame (ORF). The cytoplasmic tail contains two conserved motifs, NPIY and NPKY. Its protein comprises a signal peptide, an INB domain, an EGF-2 domain, an integrin-B-tail domain, a transmembrane domain, and an integrin-β-cyt domain. Phylogenetic and homology analyses revealed that integrin β1 is highly conserved among vertebrates. Tissue-specific expression analysis revealed that <em>SsITGβ1</em> mRNA levels were highest in liver tissue. In the stimulation experiment of <em>Edwardsiella piscicida</em>, the expression of <em>SsITGβ1</em> in spleen tissues changed significantly. Subcellular localization confirmed that <em>SsITGβ1</em> is located in the cell membrane. Furthermore, in the <em>S. schlegelii</em> intestinal cell line, assays for cell-matrix adhesion, wound healing, and flow cytometry demonstrated that the overexpression of <em>SsITGβ1</em> (via the plasmid pcDNA3.1-<em>SsITGβ1</em>) enhanced cell adhesion, promoted migration, and reduced apoptosis. In contrast, knockdown of <em>SsITGβ1</em> using small interfering RNA resulted in the opposite effects. Analysis of mRNA changes in <em>SsITGβ1</em>-regulated genes indicated that <em>SsITGβ1</em> may influence the aforementioned cellular activities via the <em>FAK</em>/<em>ILK</em>/<em>RhoA</em> and <em>Bcl-2</em>/<em>IL-1β</em>/<em>TGF-β1</em> signaling pathways. This study offers a comprehensive characterization and functional analysis of integrin β1 in <em>S. schlegelii</em>, establishing a foundation for investigating its role in the regulatory mechanisms of immune responses and cellular activities.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105467"},"PeriodicalIF":2.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clip-domain serine proteinases and their homologs: Role in crustacean immunity","authors":"Walaiporn Charoensapsri ,&nbsp;Miti Jearaphunt ,&nbsp;Chanprapa Imjongjirak ,&nbsp;Piti Amparyup","doi":"10.1016/j.dci.2025.105465","DOIUrl":"10.1016/j.dci.2025.105465","url":null,"abstract":"<div><div>Clip-domain serine proteinases (clip-SPs) and their non-catalytic serine proteinase homologs (SPHs) constitute a highly conserved protein family that plays a critical role in the innate immunity of crustaceans. These extracellular proteolytic enzymes contain one or more N-terminal clip domains and a C-terminal serine proteinase or proteinase-like domain. Their classification as either catalytically active clip-SPs or inactive SPHs is based on the presence or absence of a conserved catalytic triad (His-Asp-Ser). This review provides a comprehensive overview of their structural features, molecular classification, and occurrence across diverse crustacean species. Clip-SPs and SPHs act as key modulators of the innate immune system. They mediate proteolytic activation of the prophenoloxidase (proPO) cascade, which drives the melanization response, and coordinate antimicrobial and antiviral defenses to limit pathogen proliferation and promote immune clearance. They also influence the transcription of antimicrobial peptides (AMPs), likely via molecular cross-talk between the proPO cascade and Toll signaling pathway. This review summarizes current knowledge of clip-SPs and SPHs in crustaceans, emphasizing their integrative function and potential relevance to host defense mechanisms.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105465"},"PeriodicalIF":2.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the regulatory role of Nrf2 in Beclin1-mediated autophagy in freshwater bivalve Hyriopsis cumingii","authors":"Yuzhuo He,&nbsp;Shaoyu Hu,&nbsp;Qinglin Yang,&nbsp;Xiaobo Yu,&nbsp;Yanhong Li,&nbsp;Zhengli Wu","doi":"10.1016/j.dci.2025.105460","DOIUrl":"10.1016/j.dci.2025.105460","url":null,"abstract":"<div><div><em>Beclin1</em> is a central regulator of autophagy and cellular homeostasis, playing a critical role in the adaptation to environmental stress. Despite its importance, the expression patterns and regulatory mechanisms of <em>Beclin1</em> in bivalves remain poorly understood. In this work, we cloned the <em>Beclin1</em> gene from <em>Hyriopsis cumingii</em> (designated <em>HcBeclin1</em>) and investigated its regulatory interaction with nuclear factor erythroid 2-related factor 2 (<em>Hc</em>Nrf2). The full-length <em>HcBeclin1</em> cDNA was 1365 bp and encoded a conserved open reading frame, sharing high sequence similarity with homologous genes in other species. Reverse transcription quantitative PCR analysis revealed that <em>HcBeclin1</em> expression was significantly upregulated in the gills, hepatopancreas, and hemolymph in response to H₂O₂-induced oxidative stress. Notably, the expression levels of <em>HcBeclin1</em> in the gills and hemolymph correlated positively with those of <em>HcNrf2</em> under stress conditions. RNA interference experiments demonstrated that silencing <em>HcNrf2</em> led to a marked downregulation of <em>HcBeclin1</em>, suggesting a regulatory relationship. The promoter region of <em>HcBeclin1</em> was obtained through high-efficiency Thermal Asymmetric Interlaced PCR, revealing five putative <em>Hc</em>Nrf2 binding sites. Luciferase reporter assays identified a critical binding site within −937 to −663 bp region of the promoter, which was essential for transcriptional activation. Functional assays further confirmed that <em>Hc</em>Nrf2 regulated <em>HcBeclin1</em> expression by binding to specific cis-acting elements within its promoter. These findings offer new insights into the molecular mechanisms underlying autophagy regulation in bivalves and highlight the pivotal role of <em>HcBeclin1</em> in mediating cellular responses to oxidative stress.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105460"},"PeriodicalIF":2.4,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Putative orthologues of natural killer cell receptors and ligands in shrimp","authors":"Liping Zheng ,&nbsp;Omkar Vijay Byadgi ,&nbsp;Mostafa Rakhshaninejad ,&nbsp;Hans Nauwynck","doi":"10.1016/j.dci.2025.105459","DOIUrl":"10.1016/j.dci.2025.105459","url":null,"abstract":"<div><div>In vertebrates, natural killer (NK) cells play an important role in innate immunity. They detect target cells by receptor-ligand interactions and become activated or inhibited by a variety of cell surface activating and inhibitory receptors. In invertebrates, the defense against pathogens relies entirely on innate immunity, of which haemocytes are the only immune cells. In shrimp, two subpopulations of non-adherent, non-phagocytic, lymphocyte-like haemocytes were identified to have an NK-like activity. It is postulated now that they also may use the NK-like recognition mechanism by receptors and ligands. By searching for NK receptor genes in the shrimp genome database (already identified and described as such or by aligning the sequence of mammalian NK receptors with the shrimp genome), several receptors, such as tumor necrosis factor receptor superfamily (TNFRSF), semaphorins (SEMAs), sialic acid-binding immunoglobulin-like lectins (SIGLECs) and their corresponding ligands were discovered. The domain structure of these receptors and ligands were described and compared with those of mammalian domains, revealing 13 %–64 % similarity between them. The identification of shrimp NK cell surface receptors and their signaling pathways is primarily summarized in this review. Understanding the molecular interactions between NK-like receptors and their corresponding ligands in shrimp will help enhance our understanding of invertebrate immunology and controlling infectious diseases in shrimp aquaculture.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105459"},"PeriodicalIF":2.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molt dampens pro-inflammatory cytokine production during the acute phase response to lipopolysaccharide in the domestic chicken (Gallus domesticus)","authors":"A.M. DeRogatis,&nbsp;R.A. Hernandez,&nbsp;J.C. Suico,&nbsp;K.C. Klasing","doi":"10.1016/j.dci.2025.105461","DOIUrl":"10.1016/j.dci.2025.105461","url":null,"abstract":"<div><div>There are a variety of nutritionally costly life stages that birds must balance with investments in the immune system, yet much remains unknown about how the process of molt impacts immunity. For chickens, similar to other birds, molt is characterized by periodic feather shedding and replacement along with broader physiological changes like reproductive quiescent. To investigate how molt modulates innate immunity, mRNA expression and the plasma concentration of key cytokines were evaluated following an acute phase response induced by lipopolysaccharide (LPS-APR). Laying hens were challenged with an intraperitoneal injection of LPS at 1.5 mg/kg of body weight during the peak feather production period of an induced molt. Cytokine expression was evaluated in the liver, spleen, and plasma 4 h post-injection. Molting hens had reduced expression of certain pro-inflammatory cytokines, with the most significant reduction occurring in expression of IL-6 in the liver. This trend occurred even when the number of molted feathers varied in the fully feather UCD-003 or sparsely feathered Scaleless High hens. Importantly, molting hens failed to significantly increase plasma levels of IL-1β and IL-6 to those observed in the non-molting birds in response to LPS. A reduction in the expression of key pro-inflammatory cytokines, especially those involved in the febrile response and hepatic immune function, suggest that molt may reduce the availability of nutrients and energy needed to support the acute phase response to LPS. This attenuation is likely a mechanism to allow both molt and an innate immune response to progress during an energetic or nutritional bottleneck.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"172 ","pages":"Article 105461"},"PeriodicalIF":2.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroanatomical profiling of the rainbow trout brain parenchyma and meninges reveals specialized immune niches and region-specific hubs for bacterial immune surveillance","authors":"Amir Mani,&nbsp;Narmin Musayeva,&nbsp;Irene Salinas","doi":"10.1016/j.dci.2025.105454","DOIUrl":"10.1016/j.dci.2025.105454","url":null,"abstract":"<div><div>Several studies have described immune responses in the teleost brain and meninges during infection, however, fundamental studies that systematically dissect how different regions of the brain maintain immune homeostasis in teleosts are missing. Here we present an in-depth investigation of the immune status of the brain parenchyma and meninges of juvenile rainbow trout (<em>Oncorhynchus mykiss</em>) at the steady state. We dissected four parenchymal brain regions including olfactory bulbs (OB), telencephalon (Tel), optic tectum (OT) and cerebellum (Cer) and its corresponding dorsal meninges. Gene expression analyses revealed higher expression of all studied immune gene markers in the meninges compared to the adjacent parenchymal areas. In the parenchyma, <em>il1b, tnfa, ighd, ighm, ight, c3ra, icam1</em>, and <em>vcam1</em> expression were highest in the OB compared to other regions. Interestingly, <em>il6</em> and <em>il10</em> expression was lowest in the OB and higher in the posterior brain. <em>Nod2a</em> and <em>nod2b</em> expression levels were highest in the OT, a finding that was confirmed <em>by in situ</em> hybridization. <em>cd45 in situ</em> hybridization revealed that most of the cd45^high (immune cells) in the brain are located at the borders of the brain parenchyma (glia limitans superficialis). The present study demonstrates the presence of regional differences in the brain immune system of rainbow trout at homeostasis and identifies previously unknown hubs poised for specialized detection of microbial products.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105454"},"PeriodicalIF":2.4,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145014089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolutionary and molecular characterization of fish specific decoy receptor 3 isoforms (TNFRSF6B.1 and 2) in rock bream (Oplegnathus fascatus)","authors":"Sungjae Ko,&nbsp;Suhee Hong","doi":"10.1016/j.dci.2025.105453","DOIUrl":"10.1016/j.dci.2025.105453","url":null,"abstract":"<div><div>TNFRSF6B, commonly referred to as decoy receptor 3, interacts with TNFSF6, TNFSF14, and TNFSF15, thereby imparting anti-apoptotic and anti-inflammatory properties. This study identifies two isoforms, TNFRSF6B.1 and TNFRSF6B.2, in rock bream (RB) and explores their evolutionary and molecular attributes. Phylogenetic analysis indicates that the teleost isoforms TNFRSF6B.1 and TNFRSF6B.2 have diverged from ancestral forms of TNFRSF6B and TNFRSF11B. Notably, the coelacanth TNFRSF6B is positioned phylogenetically closer to frogs, while TNFRSF6B.2 branches off from TNFRSF11B. Synteny analysis reveals that the TNFRSF6B isoforms in rainbow trout are situated within the conserved mammalian TNFRSF6B locus, whereas the teleost TNFRSF6B.1 and TNFRSF6B.2 genes are located within the conserved TNFRSF11B locus. However, the amino acid sequences of TNFRSF6B.1 and TNFRSF6B.2 exhibit greater similarity to TNFRSF6B than to TNFRSF11B, with homology percentages of 33.92 % and 36.31 % for TNFRSF6B, and 27.94 % and 29.98 % for TNFRSF11B, respectively. Furthermore, protein-protein interaction analyses demonstrate associations with typical ligands of TNFRSF6B, including TNFSF14, TNFSF6, TNFSF5, and TNFSF15. Tertiary structure modeling of receptor-ligand interactions has also identified conserved TNFSF15-binding residues in both TNFRSF6B.1 and TNFRSF6B.2. In healthy rock bream, TNFRSF6B.1 expression is predominantly observed in gill and body kidney, while TNFRSF6B.2 expression is highest in fin, followed by skin and gill. <em>In vitro,</em> experiment reveals that TNFRSF6B.1 expression is significantly upregulated by calcium ionophores at 4 and 8 h, as well as by LPS at 8 or 12 h. Conversely, TNFRSF6B.2 exhibits a more pronounced response to concanavalin A and phytohemagglutinin at 4 or 12 h compared to TNFRSF6B.1. Both isoforms are upregulated in response to recombinant RB-TNFSF15 protein (RB-rTNFSF15). <em>In vivo</em>, expression levels of these genes are not significantly altered by RB-rTNFSF15 in head kidney, spleen, and blood, but are significantly upregulated in liver. In gill, RB-TNFRSF6B.1 expression is upregulated, whereas RB-TNFRSF6B.2 expression is downregulated. These findings suggest that TNFRSF6B.1 and TNFRSF6B.2 play roles in inflammatory responses and mucosal immunity, particularly in relation to TNFSF15, akin to mechanisms observed in humans.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"171 ","pages":"Article 105453"},"PeriodicalIF":2.4,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}