首页 > 最新文献

Diseases of aquatic organisms最新文献

英文 中文
Identification and antifungal sensitivity of Fusarium species isolated from piscine hosts. 从鱼类宿主中分离出的镰刀菌种的鉴定和抗真菌敏感性。
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-29 DOI: 10.3354/dao03809
Victoria Chanu Khangembam, Dimpal Thakuria, Ritesh Shantilal Tandel, Vinita Pant, Nityanand Pandey, Pramod Kumar Pandey

Fusarium is a huge genus of filamentous fungi that has the potential to cause emerging diseases. Members of this genus can cause infections in plants, animals and humans. Here, we report the isolation of F. oxysporum and F. equiseti from 2 important fish species, Oncorhynchus mykiss (rainbow trout) and Tor putitora (golden mahseer), respectively. F. oxysporum has emerged as a significant fungal pathogen causing infection in many fish. However, F. equiseti has been isolated mainly from plants. As far as the available literatures are concerned, this is the first report on the isolation of F. oxysporum and F. equiseti from these hosts. The isolates were identified based on growth morphology and microscopic observation. F. oxysporum produced violet pigmentation on potato dextrose agar, while F. equiseti had yellow colouration. F. oxysporum produced 1- to 2-celled microconidia along with straight or curved macroconidia having 3 to 4 septa. F. equiseti produced abundant macroconidia with 4 or more septa. Species were further confirmed based on the nucleotide sequences of the internal transcribed spacer region. In a molecular phylogeny analysis, F. oxysporum and F. equiseti formed 2 different clades. In an antifungal sensitivity assay, F. oxysporum was found to be susceptible to clotrimazole with a minimum inhibitory concentration of 1.0 µg ml-1, whereas F. equiseti was susceptible to clotrimazole, ketoconazole and fluconazole. Overall, the main findings of this study are the infection of new hosts by Fusarium species and the limited activity of many antifungal drugs against these pathogens.

镰刀菌是一个庞大的丝状真菌属,有可能导致新出现的疾病。该属的成员可导致植物、动物和人类感染。在此,我们报告了分别从两种重要鱼类 Oncorhynchus mykiss(虹鳟鱼)和 Tor putitora(金鳟鱼)中分离出 F. oxysporum 和 F. equiseti 的情况。F. oxysporum 已成为导致许多鱼类感染的重要真菌病原体。然而,F. equiseti 主要是从植物中分离出来的。就现有文献而言,这是首次从这些寄主中分离出 F. oxysporum 和 F. equiseti 的报告。根据生长形态和显微镜观察对分离物进行了鉴定。F. oxysporum 在马铃薯葡萄糖琼脂上产生紫色色素,而 F. equiseti 则呈黄色。F. oxysporum 产生 1 至 2 室的微菌丝,以及有 3 至 4 个隔膜的直或弯曲的大菌丝。F. equiseti 产生大量具有 4 个或更多隔膜的大锥体。根据内部转录间隔区的核苷酸序列进一步确认了物种。在分子系统进化分析中,F. oxysporum 和 F. equiseti 形成了两个不同的支系。在抗真菌敏感性试验中,发现 F. oxysporum 对克霉唑敏感,最低抑菌浓度为 1.0 µg ml-1,而 F. equiseti 对克霉唑、酮康唑和氟康唑敏感。总之,这项研究的主要发现是镰刀菌感染了新的寄主,而许多抗真菌药物对这些病原体的活性有限。
{"title":"Identification and antifungal sensitivity of Fusarium species isolated from piscine hosts.","authors":"Victoria Chanu Khangembam, Dimpal Thakuria, Ritesh Shantilal Tandel, Vinita Pant, Nityanand Pandey, Pramod Kumar Pandey","doi":"10.3354/dao03809","DOIUrl":"https://doi.org/10.3354/dao03809","url":null,"abstract":"<p><p>Fusarium is a huge genus of filamentous fungi that has the potential to cause emerging diseases. Members of this genus can cause infections in plants, animals and humans. Here, we report the isolation of F. oxysporum and F. equiseti from 2 important fish species, Oncorhynchus mykiss (rainbow trout) and Tor putitora (golden mahseer), respectively. F. oxysporum has emerged as a significant fungal pathogen causing infection in many fish. However, F. equiseti has been isolated mainly from plants. As far as the available literatures are concerned, this is the first report on the isolation of F. oxysporum and F. equiseti from these hosts. The isolates were identified based on growth morphology and microscopic observation. F. oxysporum produced violet pigmentation on potato dextrose agar, while F. equiseti had yellow colouration. F. oxysporum produced 1- to 2-celled microconidia along with straight or curved macroconidia having 3 to 4 septa. F. equiseti produced abundant macroconidia with 4 or more septa. Species were further confirmed based on the nucleotide sequences of the internal transcribed spacer region. In a molecular phylogeny analysis, F. oxysporum and F. equiseti formed 2 different clades. In an antifungal sensitivity assay, F. oxysporum was found to be susceptible to clotrimazole with a minimum inhibitory concentration of 1.0 µg ml-1, whereas F. equiseti was susceptible to clotrimazole, ketoconazole and fluconazole. Overall, the main findings of this study are the infection of new hosts by Fusarium species and the limited activity of many antifungal drugs against these pathogens.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Shewanella putrefaciens as an emerging pathogen of hepatopancreas necrosis disease in Chinese mitten crab Eriocheir sinensis. 中华绒螯蟹肝胰腺坏死病的新病原体--普氏雪旺菌。
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-29 DOI: 10.3354/dao03811
Jie Liu, Chenhao Teng, Xurui Zheng, La Xu, Haipeng Cao, Chunlei Gai

Shewanella putrefaciens has been recognized as an emerging important pathogen in aquaculture. However, scarce information is available on the characterization and microbial control of S. putrefaciens as a causal agent of hepatopancreas necrosis disease in Chinese mitten crab Eriocheir sinensis. In this study, a multi-resistant S. putrefaciens isolate (DZ-A) was identified as a causal pathogen of hepatopancreas necrosis disease in Chinese mitten crabs. It showed a lethal dose (LD50) of 2.20 × 105 CFU ml-1 in Chinese mitten crabs, and multiple resistance to aminoglycoside, chloramphenicol, macrolide, penicillin, peptide, and tetracycline antimicrobials. In addition, Bdellovibrio powder exhibited a significant antibacterial effect against the pathogenic S. putrefaciens, and conferred significant protection to challenged Chinese mitten crabs with relative percentage survivals of 80.00% to 93.33% via significant improvement in their immune response and antioxidant capability. The findings of this study provide valuable insights into the phenotypic characterization and biological control of pathogenic S. putrefaciens in Chinese mitten crabs.

腐生雪旺氏菌已被认为是水产养殖中一种新出现的重要病原体。然而,有关中华绒螯蟹肝胰腺坏死病病原S. putrefaciens的特征和微生物控制的信息却很少。在这项研究中,一个多重耐药的中华绒螯蟹分离株(DZ-A)被确定为中华绒螯蟹肝胰腺坏死病的病原体。它对中华绒螯蟹的致死剂量(LD50)为 2.20 × 105 CFU ml-1,并对氨基糖苷类、氯霉素、大环内酯类、青霉素、肽类和四环素类抗菌药具有多重耐药性。此外,Bdellovibrio 菌粉对致病性 S. putrefaciens 有显著的抗菌作用,并通过显著改善中华绒螯蟹的免疫反应和抗氧化能力,为中华绒螯蟹提供显著的保护,相对存活率为 80.00% 至 93.33%。这项研究的结果为中华绒螯蟹致病性 S. putrefaciens 的表型特征和生物防治提供了有价值的见解。
{"title":"Shewanella putrefaciens as an emerging pathogen of hepatopancreas necrosis disease in Chinese mitten crab Eriocheir sinensis.","authors":"Jie Liu, Chenhao Teng, Xurui Zheng, La Xu, Haipeng Cao, Chunlei Gai","doi":"10.3354/dao03811","DOIUrl":"https://doi.org/10.3354/dao03811","url":null,"abstract":"<p><p>Shewanella putrefaciens has been recognized as an emerging important pathogen in aquaculture. However, scarce information is available on the characterization and microbial control of S. putrefaciens as a causal agent of hepatopancreas necrosis disease in Chinese mitten crab Eriocheir sinensis. In this study, a multi-resistant S. putrefaciens isolate (DZ-A) was identified as a causal pathogen of hepatopancreas necrosis disease in Chinese mitten crabs. It showed a lethal dose (LD50) of 2.20 × 105 CFU ml-1 in Chinese mitten crabs, and multiple resistance to aminoglycoside, chloramphenicol, macrolide, penicillin, peptide, and tetracycline antimicrobials. In addition, Bdellovibrio powder exhibited a significant antibacterial effect against the pathogenic S. putrefaciens, and conferred significant protection to challenged Chinese mitten crabs with relative percentage survivals of 80.00% to 93.33% via significant improvement in their immune response and antioxidant capability. The findings of this study provide valuable insights into the phenotypic characterization and biological control of pathogenic S. putrefaciens in Chinese mitten crabs.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relationship between skin and body condition in three species of baleen whales. 三种须鲸的皮肤与身体状况之间的关系。
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-15 DOI: 10.3354/dao03808
Joyce Neves, Séverine Methion, Bruno Díaz López

The assessment of free-ranging cetacean health through the study of skin conditions using photographs has gained prominence in recent years. However, little attention has been given to the relationships between cetacean skin conditions, species, and body condition. To explore this relationship among baleen whale species along the northwestern coast of Spain, we employed a non-invasive method involving photograph analysis. In this study, we examined skin conditions (including injuries, epizoites and ectoparasites, pigmentation disorders, skin lesions, and anatomical malformations) and body condition (overall physical contours and form, as an indicator of nutritional status and health) in 3 species of whales (blue, fin, and minke whales). This methodology facilitated the identification of 29 subcategories of distinct skin conditions and an assessment of body condition over a 5 yr period (2017 to 2021). In our study, we present evidence linking hypopigmentation, protruding pieces of tissue, and tattoo-like lesions to 'Poor' body condition in the 3 baleen whale species. Fin whales exhibited a higher susceptibility to mottling (prevalence = 17.7%), while blue whales were more prone to starbursts (prevalence = 90.5%). Additionally, we found a significant relationship between skin condition diversity and individual body condition. Our findings contribute valuable information to the broader understanding of the health status of baleen whales. Further investigations are necessary to delve into the etiology of the documented skin conditions and their potential implications for individual survival. This study serves as a foundation for ongoing research aimed at advancing our comprehension of these findings.

近年来,通过照片研究皮肤状况来评估放养鲸目动物健康状况的方法越来越受到重视。然而,人们很少关注鲸目动物皮肤状况、物种和身体状况之间的关系。为了探索西班牙西北海岸须鲸物种之间的这种关系,我们采用了一种非侵入性的方法,包括照片分析。在这项研究中,我们检测了 3 种鲸鱼(蓝鲸、长须鲸和小须鲸)的皮肤状况(包括外伤、外寄生虫和体外寄生虫、色素沉着病、皮肤损伤和解剖畸形)和身体状况(作为营养状况和健康指标的整体身体轮廓和形态)。这种方法有助于确定 29 个不同皮肤状况的子类别,并对 5 年内(2017 年至 2021 年)的身体状况进行评估。在我们的研究中,我们提出了将色素沉着、组织块突出和纹身样病变与 3 种须鲸的 "不良 "身体状况联系起来的证据。长须鲸更容易出现斑纹(发生率=17.7%),而蓝鲸更容易出现星斑(发生率=90.5%)。此外,我们还发现皮肤状况多样性与个体身体状况之间存在显著关系。我们的发现为更广泛地了解须鲸的健康状况提供了有价值的信息。有必要进行进一步调查,以深入了解所记录的皮肤状况的病因及其对个体生存的潜在影响。这项研究为我们正在进行的研究奠定了基础,旨在加深我们对这些发现的理解。
{"title":"Relationship between skin and body condition in three species of baleen whales.","authors":"Joyce Neves, Séverine Methion, Bruno Díaz López","doi":"10.3354/dao03808","DOIUrl":"https://doi.org/10.3354/dao03808","url":null,"abstract":"<p><p>The assessment of free-ranging cetacean health through the study of skin conditions using photographs has gained prominence in recent years. However, little attention has been given to the relationships between cetacean skin conditions, species, and body condition. To explore this relationship among baleen whale species along the northwestern coast of Spain, we employed a non-invasive method involving photograph analysis. In this study, we examined skin conditions (including injuries, epizoites and ectoparasites, pigmentation disorders, skin lesions, and anatomical malformations) and body condition (overall physical contours and form, as an indicator of nutritional status and health) in 3 species of whales (blue, fin, and minke whales). This methodology facilitated the identification of 29 subcategories of distinct skin conditions and an assessment of body condition over a 5 yr period (2017 to 2021). In our study, we present evidence linking hypopigmentation, protruding pieces of tissue, and tattoo-like lesions to 'Poor' body condition in the 3 baleen whale species. Fin whales exhibited a higher susceptibility to mottling (prevalence = 17.7%), while blue whales were more prone to starbursts (prevalence = 90.5%). Additionally, we found a significant relationship between skin condition diversity and individual body condition. Our findings contribute valuable information to the broader understanding of the health status of baleen whales. Further investigations are necessary to delve into the etiology of the documented skin conditions and their potential implications for individual survival. This study serves as a foundation for ongoing research aimed at advancing our comprehension of these findings.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Francisella sciaenopsi sp. nov. isolated from diseased red drum Sciaenops ocellatus in Florida, USA. Francisella sciaenopsi sp.
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-15 DOI: 10.3354/dao03803
Miku Kawahara, Theresa T Cody, Roy P E Yanong, Eileen Henderson, Zeinab Yazdi, Esteban Soto

Piscine francisellosis is one of the most important bacterial diseases affecting various fish species worldwide. Francisella orientalis, F. noatunensis, and F. salimarina (F. marina) have been reported as etiological agents of disease in fish. A Francisella sp. was isolated from several diseased red drum Sciaenops ocellatus experiencing morbidity in Florida, USA, in 2008. In this study, molecular and phenotypic characterization of the recovered isolate was conducted. Phenotypically, the isolate showed a biochemical reaction profile distinct from that of F. orientalis and F. salimarina. Although the 16S rRNA sequence of this isolate shared 99.61% identity to the type strain of F. philomiragia O#319LT, whole genome analysis (average nucleotide identity <95%; digital DNA-DNA hybridization <70%) and a multilocus sequence analysis of 8 concatenated housekeeping genes in comparison with other Francisella spp. indicated that this isolate was a novel Francisella species, more closely related to F. orientalis. Immersion, intracoelomic injection, and co-habitation challenges using a Nile tilapia Oreochromis niloticus fingerling model of infection were done to investigate virulence in a piscine model. Variably pigmented granulomas and pigmented macrophage aggregates were observed in the kidneys and spleens of the challenged fish, but no mortality was recorded during the 15 d challenge period, suggesting that this novel Francisella sp. might be an opportunistic pathogen of fish. Based on the phenotypic and genotypic differences from other Francisella spp. observed in this study, we propose the name Francisella sciaenopsi sp. nov. for this novel isolate.

鱼类弗朗西斯菌病是影响全球各种鱼类的最重要的细菌性疾病之一。据报道,东方弗朗西斯菌、Noatunensis 弗朗西斯菌和 Salimarina 弗朗西斯菌(F. marina)是鱼类疾病的病原体。2008 年在美国佛罗里达州,从几条发病的红鼓(Sciaenops ocellatus)中分离出了弗朗西斯菌。本研究对该分离菌进行了分子和表型鉴定。从表型上看,该分离株的生化反应特征与 F. orientalis 和 F. salimarina 不同。虽然该分离株的 16S rRNA 序列与 F. philomiragia O#319LT 的模式菌株有 99.61% 的一致性,但全基因组分析(平均核苷酸一致性
{"title":"Francisella sciaenopsi sp. nov. isolated from diseased red drum Sciaenops ocellatus in Florida, USA.","authors":"Miku Kawahara, Theresa T Cody, Roy P E Yanong, Eileen Henderson, Zeinab Yazdi, Esteban Soto","doi":"10.3354/dao03803","DOIUrl":"https://doi.org/10.3354/dao03803","url":null,"abstract":"<p><p>Piscine francisellosis is one of the most important bacterial diseases affecting various fish species worldwide. Francisella orientalis, F. noatunensis, and F. salimarina (F. marina) have been reported as etiological agents of disease in fish. A Francisella sp. was isolated from several diseased red drum Sciaenops ocellatus experiencing morbidity in Florida, USA, in 2008. In this study, molecular and phenotypic characterization of the recovered isolate was conducted. Phenotypically, the isolate showed a biochemical reaction profile distinct from that of F. orientalis and F. salimarina. Although the 16S rRNA sequence of this isolate shared 99.61% identity to the type strain of F. philomiragia O#319LT, whole genome analysis (average nucleotide identity <95%; digital DNA-DNA hybridization <70%) and a multilocus sequence analysis of 8 concatenated housekeeping genes in comparison with other Francisella spp. indicated that this isolate was a novel Francisella species, more closely related to F. orientalis. Immersion, intracoelomic injection, and co-habitation challenges using a Nile tilapia Oreochromis niloticus fingerling model of infection were done to investigate virulence in a piscine model. Variably pigmented granulomas and pigmented macrophage aggregates were observed in the kidneys and spleens of the challenged fish, but no mortality was recorded during the 15 d challenge period, suggesting that this novel Francisella sp. might be an opportunistic pathogen of fish. Based on the phenotypic and genotypic differences from other Francisella spp. observed in this study, we propose the name Francisella sciaenopsi sp. nov. for this novel isolate.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RNA interference targeting WSSV ribonucleotide reductase 2 provides long-term protection against infection in Litopenaeus vannamei. 以 WSSV 核糖核苷酸还原酶 2 为靶点的 RNA 干扰可提供长期保护,防止万年青感染。
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-15 DOI: 10.3354/dao03805
Soon Joo Hong, Ki Hong Kim

Many studies have demonstrated that long double-stranded RNAs (dsRNAs) targeting essential genes of white spot syndrome virus (WSSV) can induce a sequence-specific antiviral RNA interference (RNAi) response in shrimp, thereby offering protection against WSSV infection. However, further experimental data on the required dose of dsRNAs and the duration of protection from a single administration are necessary to establish RNAi-mediated methods as effective and practical antiviral measures. In this study, we evaluated the protective efficacy and the duration of protection provided by a single administration of various doses of long dsRNA targeting WSSV ribonucleotide reductase 2 (rr2) in white-leg shrimp Litopenaeus vannamei. The protective efficacy of long dsRNA targeting WSSV rr2 was not diminished by the reduction of the dose to 100 ng g-1 of body weight, suggesting that a relatively low dose can effectively induce an RNAi response in shrimp. Furthermore, shrimp were well-protected against WSSV challenges for up to 4 wk post-administration of the rr2-targeting long dsRNA, although the protective effect almost disappeared at 6 wk post-administration. These results suggest that long dsRNAs can provide protection against WSSV for at least 1 mo, and monthly administration of long dsRNAs could serve as a long-term protective strategy for shrimp against WSSV.

许多研究表明,针对白斑综合征病毒(WSSV)重要基因的长双链 RNA(dsRNA)可诱导对虾产生序列特异性抗病毒 RNA 干扰(RNAi)反应,从而提供抗 WSSV 感染的保护。然而,要将 RNAi 介导的方法确立为有效、实用的抗病毒措施,还需要关于 dsRNAs 所需剂量和单次给药的保护持续时间的进一步实验数据。在这项研究中,我们评估了在南美白对虾(Litopenaeus vannamei)体内单次施用不同剂量的靶向 WSSV 核糖核苷酸还原酶 2(rr2)的长 dsRNA 的保护效力和保护持续时间。将针对 WSSV rr2 的长 dsRNA 的剂量降低到 100 纳克 g-1 体重后,其保护效力并没有减弱,这表明相对较低的剂量就能有效诱导对虾产生 RNAi 反应。此外,对虾在服用 rr2 靶向长 dsRNA 长达 4 周后仍能很好地抵御 WSSV 的挑战,但在服用 6 周后保护作用几乎消失。这些结果表明,长 dsRNAs 可为对虾提供至少 1 个月的 WSSV 保护,每月施用长 dsRNAs 可作为对虾抗 WSSV 的长期保护策略。
{"title":"RNA interference targeting WSSV ribonucleotide reductase 2 provides long-term protection against infection in Litopenaeus vannamei.","authors":"Soon Joo Hong, Ki Hong Kim","doi":"10.3354/dao03805","DOIUrl":"10.3354/dao03805","url":null,"abstract":"<p><p>Many studies have demonstrated that long double-stranded RNAs (dsRNAs) targeting essential genes of white spot syndrome virus (WSSV) can induce a sequence-specific antiviral RNA interference (RNAi) response in shrimp, thereby offering protection against WSSV infection. However, further experimental data on the required dose of dsRNAs and the duration of protection from a single administration are necessary to establish RNAi-mediated methods as effective and practical antiviral measures. In this study, we evaluated the protective efficacy and the duration of protection provided by a single administration of various doses of long dsRNA targeting WSSV ribonucleotide reductase 2 (rr2) in white-leg shrimp Litopenaeus vannamei. The protective efficacy of long dsRNA targeting WSSV rr2 was not diminished by the reduction of the dose to 100 ng g-1 of body weight, suggesting that a relatively low dose can effectively induce an RNAi response in shrimp. Furthermore, shrimp were well-protected against WSSV challenges for up to 4 wk post-administration of the rr2-targeting long dsRNA, although the protective effect almost disappeared at 6 wk post-administration. These results suggest that long dsRNAs can provide protection against WSSV for at least 1 mo, and monthly administration of long dsRNAs could serve as a long-term protective strategy for shrimp against WSSV.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First case of ichthyophonosis in farmed rainbow trout Oncorhynchus mykiss in Serbia. 塞尔维亚首例养殖虹鳟鱼鱼鳞病病例。
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-15 DOI: 10.3354/dao03804
Vladimir Radosavljevic, Oliver Radanovic, Dimitrije Glišic, Nemanja Zdravkovic, Jelena Maksimovic-Zoric, Ksenija Nesic, Bozidar Savic, Bozidar Raškovic

Ichthyophonosis is a disease caused by the mesomycetozoean parasite Ichthyophonus hoferi that affects a variety of fish species, including rainbow trout Oncorhynchus mykiss Walbaum. This disease is characterized by granulomatous lesions and necrosis in various organs, which can have severe impacts on the health and welfare of the fish. Ichthyophonosis has been found in several parts of the world, including Europe, and is a significant concern in the aquaculture industry and for populations of wild marine fishes. The rainbow trout is a widely cultured salmonid species in many countries, including Serbia. Although the presence of I. hoferi in rainbow trout has been reported in several countries, it has never been documented in Serbia. In this article, we report the first case of ichthyophonosis in rainbow trout in Serbia.

鱼鳞病是由介壳虫寄生虫 Ichthyophonus hoferi 引起的一种疾病,影响多种鱼类,包括虹鳟鱼 Oncorhynchus mykiss Walbaum。这种疾病的特点是各种器官出现肉芽肿病变和坏死,会严重影响鱼类的健康和福利。鱼鳞病已在包括欧洲在内的世界多个地区发现,是水产养殖业和野生海洋鱼类种群的重大问题。虹鳟是包括塞尔维亚在内的许多国家广泛养殖的鲑鱼品种。虽然多个国家都有关于虹鳟鱼体内存在 I. hoferi 的报道,但在塞尔维亚却从未有过记录。本文报告了塞尔维亚首例虹鳟鱼嗜鱼鳞病病例。
{"title":"First case of ichthyophonosis in farmed rainbow trout Oncorhynchus mykiss in Serbia.","authors":"Vladimir Radosavljevic, Oliver Radanovic, Dimitrije Glišic, Nemanja Zdravkovic, Jelena Maksimovic-Zoric, Ksenija Nesic, Bozidar Savic, Bozidar Raškovic","doi":"10.3354/dao03804","DOIUrl":"https://doi.org/10.3354/dao03804","url":null,"abstract":"<p><p>Ichthyophonosis is a disease caused by the mesomycetozoean parasite Ichthyophonus hoferi that affects a variety of fish species, including rainbow trout Oncorhynchus mykiss Walbaum. This disease is characterized by granulomatous lesions and necrosis in various organs, which can have severe impacts on the health and welfare of the fish. Ichthyophonosis has been found in several parts of the world, including Europe, and is a significant concern in the aquaculture industry and for populations of wild marine fishes. The rainbow trout is a widely cultured salmonid species in many countries, including Serbia. Although the presence of I. hoferi in rainbow trout has been reported in several countries, it has never been documented in Serbia. In this article, we report the first case of ichthyophonosis in rainbow trout in Serbia.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of buccal cavity parasite Ceratothoa carinata (Isopoda, Cymothoidae) on the condition and reproduction of its host fish Japanese scad Decapterus maruadsi. 颊腔寄生虫 Ceratothoa carinata(等足目,鲤科)对宿主鱼日本鳞鯵的状态和繁殖的影响。
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-15 DOI: 10.3354/dao03806
Reina Nakano, Yuzumi Okumura, Hiroki Hata

All species of the isopod family Cymothoidae are obligate fish parasites, extracting nourishment through hematophagy and tissue consumption. To elucidate the detrimental effects of this parasitic relationship upon the host fish, we examined body length, weight of body, gonad, liver and stomach contents, and condition factor of Japanese scad Decapterus maruadsi infected with the buccal cavity parasite Ceratothoa carinata in different seasons. During the host fish's breeding season in July, the wet weight and condition factor of male and female host fish ages 1 and 2 were conspicuously diminished. No impacts were detected in September, after the breeding season. We found no impact of the parasite on the stomach content weight or signs of prey fish in the stomachs. Thus, parasite infection with C. carinata potentially diminishes the reproductive success of the host fish by negatively impacting the host's physiological condition, particularly during the breeding season.

鲤形目(Cymothoidae)等足目科的所有物种都是鱼类的寄生虫,通过食血和消耗组织来获取营养。为了阐明这种寄生关系对宿主鱼类的有害影响,我们研究了不同季节感染颊腔寄生虫Ceratothoa carinata的日本鲭(Decapterus maruadsi)的体长、体重、性腺、肝脏和胃内容物以及状态因子。在 7 月宿主鱼的繁殖季节,1、2 龄雌雄宿主鱼的湿重和体质指数明显下降。在繁殖季节过后的 9 月份,没有发现任何影响。我们没有发现寄生虫对胃内容物重量或胃中猎物鱼的迹象有任何影响。因此,寄生虫感染 C. carinata 可能会对宿主的生理状况产生负面影响,从而降低宿主鱼的繁殖成功率,尤其是在繁殖季节。
{"title":"Effects of buccal cavity parasite Ceratothoa carinata (Isopoda, Cymothoidae) on the condition and reproduction of its host fish Japanese scad Decapterus maruadsi.","authors":"Reina Nakano, Yuzumi Okumura, Hiroki Hata","doi":"10.3354/dao03806","DOIUrl":"https://doi.org/10.3354/dao03806","url":null,"abstract":"<p><p>All species of the isopod family Cymothoidae are obligate fish parasites, extracting nourishment through hematophagy and tissue consumption. To elucidate the detrimental effects of this parasitic relationship upon the host fish, we examined body length, weight of body, gonad, liver and stomach contents, and condition factor of Japanese scad Decapterus maruadsi infected with the buccal cavity parasite Ceratothoa carinata in different seasons. During the host fish's breeding season in July, the wet weight and condition factor of male and female host fish ages 1 and 2 were conspicuously diminished. No impacts were detected in September, after the breeding season. We found no impact of the parasite on the stomach content weight or signs of prey fish in the stomachs. Thus, parasite infection with C. carinata potentially diminishes the reproductive success of the host fish by negatively impacting the host's physiological condition, particularly during the breeding season.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regeneration of tunic cuticle is suppressed in edible ascidian Halocynthia roretzi contracting soft tunic syndrome. 食用腹足纲 Halocynthia roretzi 感染软鳞茎皮综合征后,鳞茎皮的再生受到抑制。
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-01 DOI: 10.3354/dao03801
Kei Nakayama, Yumiko Obayashi, Leo Munechika, Shin-Ichi Kitamura, Tetsuya Yanagida, Miho Honjo, Shoko Murakami, Euichi Hirose

Soft tunic syndrome is an infectious disease caused by the flagellate Azumiobodo hoyamushi, which severely damages the aquaculture of the edible ascidian Halocynthia roretzi. Tunic is a cellulosic extracellular matrix entirely covering the body in ascidians and other tunicates, and its dense cuticle layer covers the tunic surface as a physical barrier against microorganisms. When the tunic of intact H. roretzi individuals was cut into strips, electron-dense fibers (DFs) appeared on the cut surface of the tunic matrix and aggregated to regenerate a new cuticular layer in seawater within a few days. DF formation was partially or completely inhibited in individuals with soft tunic syndrome, and DF formation was also inhibited by the presence of some proteases, indicating the involvement of proteolysis in the process of tunic softening as well as cuticle regeneration. Using pure cultures of the causative flagellate A. hoyamushi, the expression of protease genes and secretion of some proteases were confirmed by RNA-seq analysis and a 4-methylcoumaryl-7-amide substrate assay. Some of these proteases may degrade proteins in the tunic matrix. These findings suggest that the proteases of A. hoyamushi is the key to understanding the mechanisms of cuticular regeneration inhibition and tunic softening.

软鳞片综合症是一种由鞭毛虫 "Azumiobodo hoyamushi "引起的传染病,它严重损害了可食用腹足类 Halocynthia roretzi 的养殖。鳞茎皮是一种纤维素细胞外基质,完全覆盖在腹足类和其他鳞茎类动物的身体上,其致密的角质层覆盖在鳞茎皮表面,作为抵御微生物的物理屏障。将完整的栉水母的鳞片切成条状后,鳞片基质的切面上会出现电子致密纤维(DFs),并在几天内聚集在一起,在海水中再生出新的角质层。软鳞茎皮综合征患者的电子致密纤维(DFs)形成受到部分或完全抑制,某些蛋白酶的存在也抑制了电子致密纤维的形成,这表明蛋白分解参与了鳞茎皮软化和角质层再生的过程。利用致病鞭毛虫 A. hoyamushi 的纯培养物,通过 RNA-seq 分析和 4-甲基迷迭香-7-酰胺底物测定,证实了蛋白酶基因的表达和一些蛋白酶的分泌。其中一些蛋白酶可能会降解鳞茎皮基质中的蛋白质。这些发现表明,A. hoyamushi 的蛋白酶是了解角质层再生抑制和鳞茎皮软化机制的关键。
{"title":"Regeneration of tunic cuticle is suppressed in edible ascidian Halocynthia roretzi contracting soft tunic syndrome.","authors":"Kei Nakayama, Yumiko Obayashi, Leo Munechika, Shin-Ichi Kitamura, Tetsuya Yanagida, Miho Honjo, Shoko Murakami, Euichi Hirose","doi":"10.3354/dao03801","DOIUrl":"https://doi.org/10.3354/dao03801","url":null,"abstract":"<p><p>Soft tunic syndrome is an infectious disease caused by the flagellate Azumiobodo hoyamushi, which severely damages the aquaculture of the edible ascidian Halocynthia roretzi. Tunic is a cellulosic extracellular matrix entirely covering the body in ascidians and other tunicates, and its dense cuticle layer covers the tunic surface as a physical barrier against microorganisms. When the tunic of intact H. roretzi individuals was cut into strips, electron-dense fibers (DFs) appeared on the cut surface of the tunic matrix and aggregated to regenerate a new cuticular layer in seawater within a few days. DF formation was partially or completely inhibited in individuals with soft tunic syndrome, and DF formation was also inhibited by the presence of some proteases, indicating the involvement of proteolysis in the process of tunic softening as well as cuticle regeneration. Using pure cultures of the causative flagellate A. hoyamushi, the expression of protease genes and secretion of some proteases were confirmed by RNA-seq analysis and a 4-methylcoumaryl-7-amide substrate assay. Some of these proteases may degrade proteins in the tunic matrix. These findings suggest that the proteases of A. hoyamushi is the key to understanding the mechanisms of cuticular regeneration inhibition and tunic softening.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Countrywide screening supports model-based predictions of the distribution of Batrachochytrium dendrobatidis in Ukraine. 全国范围的筛查支持基于模型的乌克兰蝙蝠病分布预测。
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-01 DOI: 10.3354/dao03802
Joanna Jakóbik, Mykola Drohvalenko, Eduardo Fernandez Melendez, Emilia Kępa, Oleksandra Klynova, Anna Fedorova, Oleksii Korshunov, Oleksii Marushchak, Oksana Nekrasova, Natalia Suriadna, Nazar Smirnov, Oksana Tkachenko, Andrii Tupikov, Christophe Dufresnes, Oleksandr Zinenko, Maciej Pabijan

The chytrid Batrachochytrium dendrobatidis (Bd) is a widespread fungus causing amphibian declines across the globe. Although data on Bd occurrence in Eastern Europe are scarce, a recent species distribution model (SDM) for Bd reported that western and north-western parts of Ukraine are highly suitable to the pathogen. We verified the SDM-predicted range of Bd in Ukraine by sampling amphibians across the country and screening for Bd using qPCR. A total of 446 amphibian samples (tissue and skin swabs) from 11 species were collected from 36 localities. We obtained qPCR-positive results for 33 samples including waterfrogs (Pelophylax esculentus complex) and fire- and yellow-bellied toads (Bombina spp.) from 8 localities. We found that Bd-positive localities had significantly higher predicted Bd habitat suitability than sites that were pathogen-free. Amplification and sequencing of the internal transcribed spacer (ITS) region of samples with the highest Bd load revealed matches with ITS haplotypes of the globally distributed BdGPL strain, and a single case of the BdASIA-2/BdBRAZIL haplotype. We found that Bd was non-randomly distributed across Ukraine, with infections present in the western and north-central forested peripheries of the country with a relatively cool, moist climate. On the other hand, our results suggest that Bd is absent or present in low abundance in the more continental central, southern and eastern regions of Ukraine, corroborating the model-predicted distribution of chytrid fungus. These areas could potentially serve as climatic refugia for Bd-susceptible amphibian hosts.

蝙蝠疫糜烂菌(Batrachochytrium dendrobatidis,Bd)是一种广泛传播的真菌,导致全球两栖动物数量下降。虽然有关 Bd 在东欧出现的数据很少,但最近的一个 Bd 物种分布模型(SDM)报告称,乌克兰西部和西北部地区非常适合这种病原体。我们在乌克兰全国范围内对两栖动物进行了采样,并使用 qPCR 对 Bd 进行了筛查,从而验证了 SDM 预测的 Bd 在乌克兰的分布范围。我们从 36 个地方收集了 11 种两栖动物的 446 份样本(组织和皮肤拭子)。我们从 8 个地点获得了 33 个样本的 qPCR 阳性结果,其中包括水蛙(Pelophylax esculentus complex)以及火腹蟾蜍和黄腹蟾蜍(Bombina spp.)。我们发现,与无病原体的地点相比,Bd 阳性地点的预测 Bd 生境适宜性要高得多。对Bd负荷最高的样本的内部转录间隔区(ITS)进行扩增和测序,发现其与全球分布的BdGPL菌株的ITS单倍型以及BdASIA-2/BdBRAZIL单倍型的单例相匹配。我们发现,Bd 在乌克兰各地的分布并不随机,该国西部和中北部的森林边缘地带气候相对凉爽、湿润,也存在感染病例。另一方面,我们的研究结果表明,在乌克兰大陆性较强的中部、南部和东部地区,Bd不存在或数量较少,这与模型预测的糜烂性真菌分布情况相吻合。这些地区有可能成为对 Bd 敏感的两栖动物宿主的气候庇护所。
{"title":"Countrywide screening supports model-based predictions of the distribution of Batrachochytrium dendrobatidis in Ukraine.","authors":"Joanna Jakóbik, Mykola Drohvalenko, Eduardo Fernandez Melendez, Emilia Kępa, Oleksandra Klynova, Anna Fedorova, Oleksii Korshunov, Oleksii Marushchak, Oksana Nekrasova, Natalia Suriadna, Nazar Smirnov, Oksana Tkachenko, Andrii Tupikov, Christophe Dufresnes, Oleksandr Zinenko, Maciej Pabijan","doi":"10.3354/dao03802","DOIUrl":"https://doi.org/10.3354/dao03802","url":null,"abstract":"<p><p>The chytrid Batrachochytrium dendrobatidis (Bd) is a widespread fungus causing amphibian declines across the globe. Although data on Bd occurrence in Eastern Europe are scarce, a recent species distribution model (SDM) for Bd reported that western and north-western parts of Ukraine are highly suitable to the pathogen. We verified the SDM-predicted range of Bd in Ukraine by sampling amphibians across the country and screening for Bd using qPCR. A total of 446 amphibian samples (tissue and skin swabs) from 11 species were collected from 36 localities. We obtained qPCR-positive results for 33 samples including waterfrogs (Pelophylax esculentus complex) and fire- and yellow-bellied toads (Bombina spp.) from 8 localities. We found that Bd-positive localities had significantly higher predicted Bd habitat suitability than sites that were pathogen-free. Amplification and sequencing of the internal transcribed spacer (ITS) region of samples with the highest Bd load revealed matches with ITS haplotypes of the globally distributed BdGPL strain, and a single case of the BdASIA-2/BdBRAZIL haplotype. We found that Bd was non-randomly distributed across Ukraine, with infections present in the western and north-central forested peripheries of the country with a relatively cool, moist climate. On the other hand, our results suggest that Bd is absent or present in low abundance in the more continental central, southern and eastern regions of Ukraine, corroborating the model-predicted distribution of chytrid fungus. These areas could potentially serve as climatic refugia for Bd-susceptible amphibian hosts.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Verminous bronchitis and pneumonia by nasal trematodes in Greater Caribbean manatees from Puerto Rico. 波多黎各大加勒比海蝠鲼鼻腔吸虫引起的虫性支气管炎和肺炎。
IF 1.1 4区 农林科学 Q3 FISHERIES Pub Date : 2024-08-01 DOI: 10.3354/dao03800
Carla I Rivera-Pérez, Antonio A Mignucci-Giannoni, Mark A Freeman, Juan M Orcera-Iglesias, Lesly J Cabrias-Contreras, Michelle M Dennis

Five adult Greater Caribbean manatees Trichechus manatus manatus were found stranded on various coasts of Puerto Rico; 2 stranded alive and 3 stranded dead. Clinical signs observed in live-stranded manatees included emaciation, weakness, bradypnea, arrhythmia, and nasal mucus discharge. Postmortem examinations revealed serosanguinous, mucohemorrhagic, or suppurative exudate in bronchi associated with luminal adult Pulmonicola cochleotrema (range: 18-182 trematodes), accompanied by pulmonary abscesses in 2 cases. Histologically, we observed eosinophilic bronchopneumonia of varying severity (n = 4) and chronic erosive to eosinophilic tracheobronchitis (n = 4) with squamous metaplasia (n = 3) and intralesional trematodes and eggs. The trematode identity was confirmed and compared through molecular analysis for the amplified 18S rDNA fragment. Comorbidities included enteric chiorchosis (n = 5), gastric heterocheilosis (n = 4), malnutrition (n = 4), trauma related to watercraft collision (n = 3), systemic toxoplasmosis (n = 1), acute bacterial peritonitis (n = 1), and interstitial nephritis (n = 1), suggesting that immunosuppression was a predisposing factor for lower respiratory tract pulmonicolosis. Based on lesion severity, clinical signs, and the presence and absence of other findings to explain death, this condition was considered the primary cause of death in 1 manatee, a contributory cause of death in 3 manatees, and an incidental finding in 1 individual. These clinicopathological descriptions will facilitate the diagnosis and clinical management of pulmonicolosis in T. manatus, a species endangered with extinction.

五只成年大加勒比海海牛 Trichechus manatus manatus 被发现搁浅在波多黎各的不同海岸;其中两只活着搁浅,三只搁浅死亡。在活体搁浅的海牛身上观察到的临床症状包括消瘦、虚弱、呼吸迟缓、心律失常和流鼻涕。尸检发现,支气管内有血清脓性、粘液性或化脓性渗出物,并伴有管腔内的成体Pulmonicola cochleotrema(范围:18-182条吸虫),其中2例伴有肺脓肿。在组织学上,我们观察到不同严重程度的嗜酸性支气管肺炎(4 例)和慢性糜烂性至嗜酸性气管支气管炎(4 例),并伴有鳞状上皮增生(3 例)和腔内吸虫和虫卵。通过对扩增的 18S rDNA 片段进行分子分析,确认并比较了吸虫身份。合并症包括肠驰豫病(5 例)、胃异蠕虫病(4 例)、营养不良(4 例)、与水上船只碰撞有关的外伤(3 例)、全身性弓形虫病(1 例)、急性细菌性腹膜炎(1 例)和间质性肾炎(1 例),这表明免疫抑制是下呼吸道肺吸虫病的易感因素。根据病变的严重程度、临床症状以及是否存在其他可解释死亡的发现,有 1 只海牛的死亡被认为是主要死因,3 只海牛的死亡是诱因,1 只海牛的死亡是偶然发现。这些临床病理描述将有助于海牛肺栓塞病的诊断和临床治疗,海牛是一种濒临灭绝的物种。
{"title":"Verminous bronchitis and pneumonia by nasal trematodes in Greater Caribbean manatees from Puerto Rico.","authors":"Carla I Rivera-Pérez, Antonio A Mignucci-Giannoni, Mark A Freeman, Juan M Orcera-Iglesias, Lesly J Cabrias-Contreras, Michelle M Dennis","doi":"10.3354/dao03800","DOIUrl":"https://doi.org/10.3354/dao03800","url":null,"abstract":"<p><p>Five adult Greater Caribbean manatees Trichechus manatus manatus were found stranded on various coasts of Puerto Rico; 2 stranded alive and 3 stranded dead. Clinical signs observed in live-stranded manatees included emaciation, weakness, bradypnea, arrhythmia, and nasal mucus discharge. Postmortem examinations revealed serosanguinous, mucohemorrhagic, or suppurative exudate in bronchi associated with luminal adult Pulmonicola cochleotrema (range: 18-182 trematodes), accompanied by pulmonary abscesses in 2 cases. Histologically, we observed eosinophilic bronchopneumonia of varying severity (n = 4) and chronic erosive to eosinophilic tracheobronchitis (n = 4) with squamous metaplasia (n = 3) and intralesional trematodes and eggs. The trematode identity was confirmed and compared through molecular analysis for the amplified 18S rDNA fragment. Comorbidities included enteric chiorchosis (n = 5), gastric heterocheilosis (n = 4), malnutrition (n = 4), trauma related to watercraft collision (n = 3), systemic toxoplasmosis (n = 1), acute bacterial peritonitis (n = 1), and interstitial nephritis (n = 1), suggesting that immunosuppression was a predisposing factor for lower respiratory tract pulmonicolosis. Based on lesion severity, clinical signs, and the presence and absence of other findings to explain death, this condition was considered the primary cause of death in 1 manatee, a contributory cause of death in 3 manatees, and an incidental finding in 1 individual. These clinicopathological descriptions will facilitate the diagnosis and clinical management of pulmonicolosis in T. manatus, a species endangered with extinction.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Diseases of aquatic organisms
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1