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Erysipelas with preferential brain and skin involvement in a Mediterranean bottlenose dolphin Tursiops truncatus. 地中海瓶鼻海豚(Tursiops truncatus)患红斑性溃疡,脑部和皮肤优先受累。
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2024-02-01 DOI: 10.3354/dao03770
Laura Martino, Bárbara Serrano, Jaume Alomar, Lola Pérez, Virginia Aragon, Alex Cobos, Maria Lourdes Abarca, Zeinab Yazdi, Esteban Soto, Mariano Domingo

Infections by Erysipelothrix rhusiopathiae occur in domestic animals and cause the disease known as 'erysipelas'. The ubiquity of Erysipelothrix spp. makes infection possible in a wide range of vertebrates and invertebrates. Cetaceans are highly susceptible to erysipelas, especially those under human care. The number of cases documented in wild cetaceans is low, the pathogenesis is incompletely understood, and the full spectrum of lesions is not well defined. The possible serotypes and species of the genus that can cause disease are unknown. In October 2022, a common bottlenose dolphin Tursiops truncatus stranded in Vilassar de Mar (Catalonia) showing skin lesions consistent with 'diamond skin disease', a characteristic lesion of erysipelas shared by swine and cetaceans. Necropsy was performed following standardized procedures, and multiple samples were taken for histopathology and bacteriology. Erysipelothrix sp. grew in pure culture in many tissue samples. Genetic characterization by multi-locus sequence analysis identified the species as E. rhusiopathiae. Histologically, the main lesions were an intense suppurative vasculitis of leptomeningeal arteries and veins with abundant intramural Gram-positive bacilli and meningeal hemorrhages. Meningeal lesions were considered the cause of death. The affected skin showed moderate suppurative dermatitis. Herein we document a case of erysipelas in a Mediterranean common bottlenose dolphin with unusual lesions in the leptomeningeal vessels and marked skin tropism. To our knowledge, this is the first case of severe brain involvement in erysipelas in a cetacean. We also provide a review of available cases in wild cetaceans, to highlight the characteristics of the disease and improve future diagnosis.

Erysipelothrix rhusiopathiae 感染发生在家畜身上,会导致被称为 "红斑狼疮 "的疾病。Erysipelothrix spp.无处不在,因此可感染多种脊椎动物和无脊椎动物。鲸目动物极易感染红斑狼疮,尤其是那些受到人类照料的鲸目动物。在野生鲸目动物中记录的病例数量很少,对其发病机理的了解也不全面,病变的全部范围也没有很好地界定。该属可能致病的血清型和物种尚不清楚。2022 年 10 月,一条普通瓶鼻海豚 Tursiops truncatus 在加泰罗尼亚的 Vilassar de Mar 搁浅,其皮肤出现与 "钻石皮病 "一致的病变,这是猪和鲸类共有的红斑性溃疡的特征性病变。按照标准程序进行了解剖,并采集了多个样本进行组织病理学和细菌学检查。在许多组织样本中,红斑病菌在纯培养物中生长。通过多焦点序列分析进行基因鉴定,确定该物种为 E. rhusiopathiae。从组织学角度看,主要病变是脑膜下动脉和静脉的剧烈化脓性血管炎,伴有大量革兰氏阳性杆菌和脑膜出血。脑膜病变被认为是死亡原因。患处皮肤出现中度化脓性皮炎。在此,我们记录了一例地中海普通瓶鼻海豚的红斑性脑膜炎病例,该病例的脑膜下血管有不寻常的病变,并有明显的皮肤趋向性。据我们所知,这是首例鲸豚红斑性痢疾严重累及大脑的病例。我们还对现有的野生鲸目动物病例进行了回顾,以突出该疾病的特点,改善未来的诊断。
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引用次数: 0
Diet-induced nephrocalcinosis in aquarium-raised juvenile spotted wolffish Anarhichas minor. 水族馆饲养的斑狼鱼幼鱼饮食引起的肾钙化症。
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2024-01-18 DOI: 10.3354/dao03769
Karine Béland, Catherine Rousseau, Stéphane Lair

Wolffish are regularly housed in aquaria, but little data on their husbandry and health is available for caretakers. High occurrence rates of nephrocalcinosis and urolithiasis have been observed in Atlantic Anarhichas lupus and spotted A. minor wolffish housed at 2 Canadian zoological institutions. To explore the effect of diet on nephrocalcinosis and urolithiasis development, a 16 mo prospective study was conducted. A total of 32 juvenile spotted wolffish were randomly assigned to one of 4 experimental groups fed exclusively with the following diet: (1) Skretting® Europa 18 pellets; (2) Mazuri® LS Aquatic Carni-Blend Diet Formula; (3) vitamin-supplemented fish-based diet, and (4) vitamin-supplemented invertebrate-based diet. Urinalysis, radiographs, and complete necropsies were performed at the end of the study. None of the wolffish developed uroliths during the study period. All specimens fed with the fish-based and invertebrate-based diets developed nephrocalcinosis, whereas this condition was seen in 12.5 and 0% of the fish in the Skretting® and Mazuri® groups, respectively. Affected wolffish often presented with oxalate crystalluria and increased radiodensity of the posterior kidneys. Urinalysis and radiographic study were considered useful in the antemortem diagnosis of nephrocalcinosis. None of the previously published risk factors for the development of nephrocalcinosis in fish were supported by the results of this study. However, nutritional analyses of the 4 diets suggest that high dietary levels of gelatin or vitamin C or low levels of vitamin E could be potential risk factors for the development of nephrocalcinosis in spotted wolffish and thus warrant further study.

狼鱼经常被饲养在水族馆中,但有关其饲养和健康状况的数据却很少提供给饲养者。据观察,加拿大两家动物园饲养的大西洋狼鱼和斑点小狼鱼的肾钙化和尿石症发生率很高。为了探索饮食对肾钙化和尿石症发生的影响,我们进行了一项为期 16 个月的前瞻性研究。共有 32 条斑点狼鱼幼鱼被随机分配到 4 个实验组中的一个,实验组只喂食以下食物:(1) Skretting® Europa 18 颗粒饲料;(2) Mazuri® LS 水产鲤鱼混合饲料配方;(3) 补充维生素的鱼类饲料;(4) 补充维生素的无脊椎动物饲料。研究结束时进行了尿液分析、X 光检查和完整的尸体解剖。在研究期间,没有一条狼鱼出现尿石症。用鱼类饲料和无脊椎动物饲料喂养的所有标本都出现了肾钙化,而 Skretting® 组和 Mazuri® 组分别有 12.5% 和 0% 的鱼出现了肾钙化。受影响的狼鱼通常表现为草酸盐结晶尿和后肾放射密度增加。尿液分析和 X 射线检查被认为有助于肾钙化症的死前诊断。之前公布的鱼类患肾癌的风险因素均未得到本研究结果的支持。然而,对 4 种饮食的营养分析表明,饮食中明胶或维生素 C 含量高或维生素 E 含量低可能是斑点狼鱼发生肾钙化的潜在风险因素,因此值得进一步研究。
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引用次数: 0
Brome mosaic virus (BMV) capsid protein virus-like particle-delivering dsRNA targeting VP28 gene of white spot syndrome virus (WSSV) through feed reduced Penaeus vannamei shrimp mortality from WSSV infection 通过饲料传递针对白斑综合征病毒(WSSV) VP28基因的衣壳蛋白病毒样颗粒可降低凡纳滨对虾对白斑综合征病毒(WSSV)感染的死亡率
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2023-10-26 DOI: 10.3354/dao03754
EA Ruiz Guerrero, I. Giffard Mena, M. Viana, S. Ramos Carreño, S. Sánchez Serrano
Numerous strategies have been investigated to combat viral infections in shrimp, specifically targeting the white spot syndrome virus (WSSV) that has caused outbreaks worldwide since the 1990s. One effective treatment involves intramuscular application of dsRNA-mediated interference against the viral capsid protein VP28. However, this approach presents challenges in terms of individual shrimp management, limiting its application on a large scale. To address this, our study aimed to evaluate the efficacy of oral delivery of protected dsRNA using chitosan nanoparticles or virus-like particles (VLPs) synthesized in brome mosaic virus (BMV). These delivery systems were administered before, during, and after WSSV infection to assess their therapeutic potential. Our findings indicate that BMV-derived VLPs demonstrated superior efficiency as nanocontainers for dsRNA delivery. Notably, the treatment involving vp28 dsRNA mixed in the feed and administered simultaneously to shrimp already infected with WSSV exhibited the highest survival rate (48%), while the infected group had a survival rate of zero, suggesting the potential efficacy of this prophylactic approach in commercial shrimp farms.
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引用次数: 0
Pathology of short-term dorsal fin tag-attachments in tagged and re-stranded short-beaked common dolphins Delphinus delphis on Cape Cod, MA. 马萨诸塞州鳕鱼角被标记和重新上链的短喙普通海豚 Delphinus delphis 的背鳍标签短期附着病理学。
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2023-10-26 DOI: 10.3354/dao03755
Treasa C Deegan, Misty Niemeyer, Kathleen M Colegrove, David S Rotstein, Sarah M Sharp

Odontocetes are difficult to study in the wild, making tagging and remote tracking a valuable practice. However, evaluations of host responses at tagging sites have been primarily limited to visual observations in the field. Here we explore the macro- and microscopic pathology of dorsal fin tag attachments in 13 stranded and released short-beaked common dolphins Delphinus delphis from Cape Cod, MA that later re-stranded and died or were euthanized 1-28 d post-tagging. Tags were attached to stranded dolphins' dorsal fins using 2 methods: core biopsy or piercing. Grossly, the piercing method resulted in epidermal compression into the dermis. One tag site had a necrotic border 28 d after application. Grossly, the biopsy method resulted in minimal to no tissue reaction. Two tag sites had granulation tissue accumulation 4 and 12 d after tagging. Histopathologic findings for all tag types and animals consisted of focal epithelial loss, dermal edema, perivascular edema, inflammation and hyperplasia, and inter- and extracellular edema in the adjacent epidermis. Minor expected pathological changes given the procedure were also observed: superficial epidermal necrosis in 3 cases, and superficial bacterial colonization in 2 cases. There was no evidence of sepsis and tagging was not related to cause of re-stranding or death in any case. These gross and histopathologic findings support previous observational conclusions in small delphinids that with appropriate sterile technique, the impacts of single pin dorsal fin tagging on the animal can be minimal and localized. Of the 2 methods, core biopsy may be a better tagging method.

齿鲸很难在野外进行研究,因此标记和远程跟踪是一种很有价值的做法。然而,对标记地点宿主反应的评估主要局限于野外肉眼观察。在此,我们探讨了马萨诸塞州鳕鱼角 13 只搁浅和释放的短喙普通海豚 Delphinus delphis 背鳍标签附着的宏观和微观病理变化。在搁浅的海豚背鳍上粘贴标签有两种方法:核心活检法或穿刺法。从外观上看,穿刺法导致表皮压入真皮层。一个标记部位在使用 28 天后出现坏死边缘。从大体上看,活检方法导致的组织反应很小甚至没有。两个标记部位在标记 4 天和 12 天后出现肉芽组织堆积。所有标记类型和动物的组织病理学结果都包括局灶性上皮脱落、真皮水肿、血管周围水肿、炎症和增生,以及邻近表皮的细胞间和细胞外水肿。此外,还观察到了手术过程中预期的轻微病理变化:3 例表皮坏死,2 例表皮细菌定植。没有败血症的迹象,标签与再次搁浅或死亡的原因无关。这些大体和组织病理学研究结果支持之前对小型三角帆鳍鱼的观察结论,即采用适当的无菌技术,单针背鳍标签对动物的影响是最小的,而且是局部性的。在这两种方法中,核心活检可能是更好的标记方法。
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引用次数: 0
B-cell small lymphocytic lymphoma in a free-ranging South American fur seal (Arctocephalus australis). 自由放养的南美洲海狗(Arctocephalus australis)的B细胞小淋巴细胞淋巴瘤。
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2023-10-12 DOI: 10.3354/dao03757
Derek B de Amorim, Paula R Ribeiro, Marcele B Bandinelli, Joanna V Z Echenique, Matheus V Bianchi, Paula R Almeida, Fernando R Spilki, Leticia F Baumbach, Luciana Sonne

Lymphomas are malignant neoplasms of the hematopoietic system arising from lymphocytes with highly variable biologic behavior. B-cell small lymphocytic lymphoma (B-SLL) is a non-Hodgkin lymphoma infrequently described in domestic and wild animals. The present study describes a case of B-SLL in a free-ranging adult male Arctocephalus australis in Brazil. The main necropsy findings included poor body condition, generalized lymphadenomegaly, severe and diffuse splenomegaly, and multiple, white to yellow nodules in the kidneys and small intestine. Histologically, these organs were partially or totally effaced by neoplastic small lymphocytes arranged in sheets, with moderate anisocytosis and anisokaryosis and a low mitotic count. These cells diffusely immunolabeled for CD79α and CD20, and were negative for CD3. A diagnosis of multicentric B-SLL was established and to the authors' knowledge, it has not been previously described in this genus.

淋巴瘤是由具有高度可变生物学行为的淋巴细胞引起的造血系统恶性肿瘤。B细胞小淋巴细胞淋巴瘤(B-SLL)是一种在家畜和野生动物中很少描述的非霍奇金淋巴瘤。本研究描述了一例在巴西自由放养的成年雄性南极Arctocephalus australis中发生的B-SL。主要尸检结果包括身体状况不佳、全身淋巴结肿大、严重和弥漫性脾肿大,以及肾脏和小肠中的多个白色至黄色结节。组织学上,这些器官被排列成片状的肿瘤性小淋巴细胞部分或完全消除,有中度的异细胞增多和异核细胞增多,有丝分裂计数低。这些细胞对CD79α和CD20进行了广泛的免疫标记,并且对CD3呈阴性。多中心B-SL的诊断已经确立,据作者所知,它以前从未在该属中描述过。
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引用次数: 0
Decline of Marteilia cochillia in Ría de Arousa may be due to increased resistance in host Cerastoderma edule. Arousaía地区的科氏马氏体的衰退可能是由于寄主Cerastoderma edule的抗性增加。
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2023-10-12 DOI: 10.3354/dao03756
David Iglesias, Asunción Cao, María J Carballal, Antonio Villalba

A huge, unprecedented mortality of cockle Cerastoderma edule caused by the protist Marteilia cochillia, which had never before been detected in Galicia (NW Spain), brought on a cockle fishery collapse in the Ría de Arousa (Galicia) in 2012. Since then, the disease dynamic pattern in the shellfish bed of Lombos do Ulla (at the inner area of that ria) involved an overwhelming annual wave of infections and subsequent cockle mass mortality that caused the near extinction of every cohort recruited to that bed. However, a pattern shift was detected among wild cohorts recruiting since 2016, with progressive declines of marteiliosis prevalence and increments in cockle survival. This suggested 2 non-exclusive hypotheses: increasing marteiliosis resistance through natural selection, and reduced abundance and/or virulence of the parasite. A field experiment was performed to assess these hypotheses by comparing marteiliosis prevalence and severity, as well as mortality, in cockles that naturally recruited to this bed in 2017 and 2018 with those of naïve cockles collected from a marteiliosis-free area and transplanted into Lombos do Ulla in 2017 and 2018. Marteiliosis prevalence and cumulative cockle mortality quickly reached very high values among the transplanted cockles, demonstrating that the parasite remained present and virulent in the area. Conversely, marteiliosis prevalence and cockle mortality were much lower in the cockles that recruited to Lombos do Ulla, suggesting increased resistance that may have been driven by natural selection. The young age at which cockles start reproduction and the very high mortality caused by marteiliosis may have enhanced natural selection.

2012年,在加利西亚(西班牙西北部)从未发现过由原生动物科氏马氏体(Marteilia cocchilia)引起的一场前所未有的巨大的死鸡场,导致了阿罗萨河(加利西亚)的一场鸡场渔业崩溃。从那时起,Lombos do Ulla贝类养殖场(位于该养殖场的内部区域)的疾病动态模式涉及每年压倒性的感染浪潮和随后的鸡群大规模死亡,导致该养殖场招募的每一个群体几乎灭绝。然而,自2016年以来,在招募的野生种群中发现了一种模式转变,马氏体病患病率逐渐下降,公鸡存活率增加。这提出了两个非排他性的假设:通过自然选择增加对马氏体的耐药性,降低寄生虫的丰度和/或毒力。为了评估这些假设,进行了一项实地实验,将2017年和2018年自然招募到该床的公鸡的马氏体病患病率、严重程度以及死亡率与2017年和18年从无马氏体病地区采集并移植到乌拉龙目的天真公鸡进行了比较。在移植的公鸡中,马氏体病的流行率和累积的公鸡死亡率很快达到了非常高的值,这表明这种寄生虫在该地区仍然存在并具有毒性。相反,在被招募到Lombos do Ulla的公鸡中,马氏体病的患病率和公鸡死亡率要低得多,这表明抵抗力的增加可能是由自然选择驱动的。公鸡开始繁殖的年龄很小,并且由马氏体病引起的死亡率很高,这可能增强了自然选择。
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引用次数: 1
Helicobacter delphinicola infection and the risk of gastric disease in common bottlenose dolphin. 常见宽吻海豚幽门螺杆菌感染与胃病风险。
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2023-09-28 DOI: 10.3354/dao03751
Takao Segawa, Yoshito Ohno, Masanori Kurita, Toshiaki Ishibashi, Motoi Yoshioka

Gastritis and gastric ulcers are well-recognized conditions in cetaceans; bacteria of the genus Helicobacter are considered the primary cause of these diseases. Dolphins have been shown to be susceptible to infection by at least 2 gastric species of Helicobacter, H. cetorum and H. delphinicola, both of which are closely related to the human pathogen H. pylori. In the present study, we evaluated the carriage rate and relationship to gastric disease of H. cetorum and H. delphinicola, based on a study population of 82 dolphins maintained at 21 facilities in Japan. Of these 82 dolphins, 79 (96.3%) and 45 (54.9%) were positive for H. cetorum and H. delphinicola, respectively; H. delphinicola infection was significantly associated with chronic gastric diseases (odds rate: 5.9; 95% CI: 2.1-16.9), but no such association was detected for H. cetorum. Of the 21 facilities, 20 (95%) and 11 (55%) housed H. cetorum- and H. delphinicola-positive dolphins, respectively, and our study suggested that the transmission between dolphins occurs quickly within pools. These findings indicate that methods will need to be established to prevent the transmission of Helicobacter infections within facilities housing dolphins.

胃炎和胃溃疡是鲸目动物公认的疾病;幽门螺杆菌属细菌被认为是这些疾病的主要原因。海豚已被证明易受至少两种胃幽门螺杆菌感染,这两种幽门螺杆菌都与人类病原体幽门螺杆菌密切相关。在本研究中,我们基于在日本21个设施中饲养的82只海豚的研究种群,评估了海燕和飞燕的携带率及其与胃病的关系。在这82只海豚中,分别有79只(96.3%)和45只(54.9%)对鲸豚和飞燕草呈阳性;飞燕草感染与慢性胃部疾病显著相关(优势率:5.9;95%可信区间:2.1-16.9),但在鹅膏菌中未检测到这种关联。在21个设施中,分别有20个(95%)和11个(55%)容纳了阳性海豚,我们的研究表明,海豚之间的传播在水池中发生得很快。这些发现表明,需要建立方法来防止海豚饲养设施内幽门螺杆菌感染的传播。
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引用次数: 0
Perkinsus olseni in green-lipped mussels Perna canaliculus: diagnostic evaluation, prevalence, and distribution. 绿唇贻贝Perna Canalitulus中的Perksus olseni:诊断评估、患病率和分布。
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2023-09-28 DOI: 10.3354/dao03750
Henry S Lane, Diana Jaramillo, Mukul Sharma

Perkinsus olseni (Perkinsidae) is a molluscan parasite notifiable to the World Organisation for Animal Health that is reported in several shellfish hosts in New Zealand, including the native green-lipped mussel Perna canaliculus. Green-lipped mussels comprise over half of New Zealand's aquaculture export value and have historically been considered free of serious diseases based on extensive histology-based surveillance. The discovery of P. olseni in green-lipped mussels has raised questions about future disease threats to green-lipped mussels, particularly under changing ocean climatic conditions. Using mussels collected from farmed (n = 358) and wild (n = 236) populations, we aimed to determine the distribution and prevalence of P. olseni in green-lipped mussels around New Zealand, and assess the performance of diagnostic tests, including real-time PCR, conventional PCR, and culture using Ray's fluid thioglycolate medium (RFTM). Prevalence and diagnostic test performance was evaluated using Bayesian latent class analysis with informative priors. The prevalence of P. olseni was 0-3%, except for 1 wild population from a harbour where prevalence was 22%. Real-time PCR had the highest diagnostic sensitivity (87%) compared to 62 and 21% for conventional PCR and RFTM, respectively. Diagnostic specificity was similar among all methods (96-98%). No mortality was observed during the study. Our results suggest that real-time PCR is the diagnostic test best suited for surveillance of P. olseni in subclinically infected green-lipped mussels under New Zealand conditions.

Perkinsus olseni(Perkinsidae)是一种软体动物寄生虫,应向世界动物卫生组织报告,据报道,它存在于新西兰的几种贝类宿主中,包括本地的绿唇贻贝Perna canaliculus。绿唇贻贝占新西兰水产养殖出口价值的一半以上,根据广泛的组织学监测,历史上一直被认为没有严重疾病。在绿唇贻贝中发现的P.olseni引发了人们对绿唇贻蚌未来疾病威胁的质疑,特别是在不断变化的海洋气候条件下。使用从养殖(n=358)和野生(n=236)种群中采集的贻贝,我们旨在确定新西兰各地绿唇贻贝中奥氏假单胞菌的分布和流行率,并评估诊断测试的性能,包括实时PCR、常规PCR和使用Ray液体巯基乙酸培养基(RFTM)培养。使用具有信息先验的贝叶斯潜在类别分析来评估患病率和诊断测试性能。除了1个来自港口的野生种群的患病率为22%外,奥氏疟原虫的患病率是0-3%。实时PCR的诊断灵敏度最高(87%),而常规PCR和RFTM分别为62%和21%。所有方法的诊断特异性相似(96-98%)。研究期间未观察到死亡。我们的研究结果表明,实时PCR是最适合在新西兰条件下监测亚临床感染的绿唇贻贝中的奥氏假单胞菌的诊断测试。
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引用次数: 0
Pseudacris regilla metamorphs acquire resistance to Batrachochytrium dendrobatidis after exposure to the killed fungus. 在暴露于被杀死的真菌后,Pseudacris regilla变态获得了对树状蜡染虫的抗性。
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2023-09-28 DOI: 10.3354/dao03753
Taegan A McMahon, Caitlin L Nordheim, Sarah E Detmering, Pieter T J Johnson, Jason R Rohr, David J Civitello

The pathogenic fungus Batrachochytrium dendrobatidis (Bd) is associated with drastic global amphibian declines. Prophylactic exposure to killed zoospores and the soluble chemicals they produce (Bd metabolites) can induce acquired resistance to Bd in adult Cuban treefrogs Osteopilus septentrionalis. Here, we exposed metamorphic frogs of a second species, the Pacific chorus frog Pseudacris regilla, to one of 2 prophylactic treatments prior to live Bd exposures: killed Bd zoospores with metabolites, killed zoospores alone, or a water control. Prior exposure to killed Bd zoospores with metabolites reduced Bd infection intensity in metamorphic Pacific chorus frogs by 60.4% compared to control frogs. Interestingly, Bd intensity in metamorphs previously exposed to killed zoospores alone did not differ in magnitude relative to the control metamorphs, nor to those treated with killed zoospores plus metabolites. Previous work indicated that Bd metabolites alone can induce acquired resistance in tadpoles, and so these findings together indicate that it is possible that the soluble Bd metabolites may contain immunomodulatory components that drive this resistance phenotype. Our results expand the generality of this prophylaxis work by identifying a second amphibian species (Pacific chorus frog) and an additional amphibian life stage (metamorphic frog) that can acquire resistance to Bd after metabolite exposure. This work increases hopes that a Bd-metabolite prophylaxis might be widely effective across amphibian species and life stages.

致病性真菌树状蝙蝠(Batrachochytrium dendrobatidis,Bd)与全球两栖动物数量急剧下降有关。预防性暴露于杀死的游动孢子及其产生的可溶性化学物质(Bd代谢产物)可以诱导成年古巴树蛙对Bd的获得性抗性。在这里,我们让第二个物种的变质蛙,太平洋合唱蛙Pseudacris regilla,在活的Bd暴露之前接受两种预防性治疗之一:用代谢产物杀死Bd游动孢子,单独杀死游动孢子,或水控制。与对照蛙相比,先前暴露于含有代谢物的杀死的Bd游动孢子可使变质太平洋合唱蛙的Bd感染强度降低60.4%。有趣的是,以前单独暴露于杀死的游动孢子的变质物的Bd强度与对照变质物的大小没有差异,也与用杀死的游动芽孢和代谢物处理的变质物没有差异。先前的研究表明,单独的Bd代谢产物可以诱导蝌蚪的获得性抗性,因此这些发现共同表明,可溶性Bd代谢物可能含有驱动这种抗性表型的免疫调节成分。我们的研究结果通过鉴定第二种两栖动物物种(太平洋合唱蛙)和一个额外的两栖动物生命阶段(变质蛙)来扩大这项预防工作的普遍性,这些两栖动物在代谢产物暴露后可以获得对Bd的抗性。这项工作增加了人们对Bd代谢产物预防可能在两栖动物物种和生命阶段广泛有效的希望。
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引用次数: 0
Comparison of methods for determining Batrachochytrium dendrobatidis zoospore viability. 水蛭壶菌游动孢子活力测定方法的比较。
IF 1.4 4区 农林科学 Q2 Medicine Pub Date : 2023-09-14 DOI: 10.3354/dao03749
Adeline Chew, Matt West, Laura A Brannelly

The emerging fungal pathogen Batrachochytrium dendrobatidis (Bd) threatens hundreds of amphibian species globally. During laboratory-based experiments it is often essential to quantify live Bd cells, but a comparison of the effectiveness of methods for counting and assessing the viability of the infectious zoospore life stage has not been done. A direct comparison of staining methods that assess viability will ensure that the most accurate and efficient method is used. Here, we compared the use of 2 relatively cheap common stains, trypan blue and methylene blue, and assessed their accuracy and precision for estimating the viability of Bd zoospores during both manual counting and colorimetric assays. We stained known proportions of killed Bd zoospores (0, 0.25, 0.50, 0.75, and 1.00) with each stain and estimated the proportion of stained (dead) and unstained (viable) cells in each sample using both manual counting and colorimetric assays. Trypan blue was found to be a much more effective stain than methylene blue for both microscopy and colorimetric assays. Additionally, counting zoospores via microscopy was both a more accurate and precise technique. We recommend using manual counts via microscopy using the trypan blue stain for assessing Bd zoospore viability.

新型真菌病原体水蛭壶菌(Batrachochytrium dendroatidis, Bd)威胁着全球数百种两栖动物。在以实验室为基础的实验中,通常必须对活的Bd细胞进行量化,但对计数和评估感染性游动孢子生命阶段活力的方法的有效性进行比较尚未完成。直接比较评估细胞活力的染色方法将确保使用最准确和有效的方法。在这里,我们比较了两种相对便宜的常用染色剂,台盼蓝和亚甲基蓝,并评估了它们在人工计数和比色测定中估计Bd游动孢子活力的准确性和精密度。我们用每种染色剂对已知比例的bdzoo孢子(0、0.25、0.50、0.75和1.00)进行染色,并使用人工计数和比色法估计每个样品中染色(死亡)和未染色(活)细胞的比例。台盼蓝被发现是比亚甲基蓝更有效的染色显微镜和比色测定。此外,通过显微镜计算游动孢子是一种更准确和精确的技术。我们建议使用人工计数,通过显微镜使用台盼蓝染色来评估Bd游动孢子的活力。
{"title":"Comparison of methods for determining Batrachochytrium dendrobatidis zoospore viability.","authors":"Adeline Chew,&nbsp;Matt West,&nbsp;Laura A Brannelly","doi":"10.3354/dao03749","DOIUrl":"https://doi.org/10.3354/dao03749","url":null,"abstract":"<p><p>The emerging fungal pathogen Batrachochytrium dendrobatidis (Bd) threatens hundreds of amphibian species globally. During laboratory-based experiments it is often essential to quantify live Bd cells, but a comparison of the effectiveness of methods for counting and assessing the viability of the infectious zoospore life stage has not been done. A direct comparison of staining methods that assess viability will ensure that the most accurate and efficient method is used. Here, we compared the use of 2 relatively cheap common stains, trypan blue and methylene blue, and assessed their accuracy and precision for estimating the viability of Bd zoospores during both manual counting and colorimetric assays. We stained known proportions of killed Bd zoospores (0, 0.25, 0.50, 0.75, and 1.00) with each stain and estimated the proportion of stained (dead) and unstained (viable) cells in each sample using both manual counting and colorimetric assays. Trypan blue was found to be a much more effective stain than methylene blue for both microscopy and colorimetric assays. Additionally, counting zoospores via microscopy was both a more accurate and precise technique. We recommend using manual counts via microscopy using the trypan blue stain for assessing Bd zoospore viability.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Diseases of aquatic organisms
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