首页 > 最新文献

Diseases of aquatic organisms最新文献

英文 中文
B-cell small lymphocytic lymphoma in a free-ranging South American fur seal (Arctocephalus australis). 自由放养的南美洲海狗(Arctocephalus australis)的B细胞小淋巴细胞淋巴瘤。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-10-12 DOI: 10.3354/dao03757
Derek B de Amorim, Paula R Ribeiro, Marcele B Bandinelli, Joanna V Z Echenique, Matheus V Bianchi, Paula R Almeida, Fernando R Spilki, Leticia F Baumbach, Luciana Sonne

Lymphomas are malignant neoplasms of the hematopoietic system arising from lymphocytes with highly variable biologic behavior. B-cell small lymphocytic lymphoma (B-SLL) is a non-Hodgkin lymphoma infrequently described in domestic and wild animals. The present study describes a case of B-SLL in a free-ranging adult male Arctocephalus australis in Brazil. The main necropsy findings included poor body condition, generalized lymphadenomegaly, severe and diffuse splenomegaly, and multiple, white to yellow nodules in the kidneys and small intestine. Histologically, these organs were partially or totally effaced by neoplastic small lymphocytes arranged in sheets, with moderate anisocytosis and anisokaryosis and a low mitotic count. These cells diffusely immunolabeled for CD79α and CD20, and were negative for CD3. A diagnosis of multicentric B-SLL was established and to the authors' knowledge, it has not been previously described in this genus.

淋巴瘤是由具有高度可变生物学行为的淋巴细胞引起的造血系统恶性肿瘤。B细胞小淋巴细胞淋巴瘤(B-SLL)是一种在家畜和野生动物中很少描述的非霍奇金淋巴瘤。本研究描述了一例在巴西自由放养的成年雄性南极Arctocephalus australis中发生的B-SL。主要尸检结果包括身体状况不佳、全身淋巴结肿大、严重和弥漫性脾肿大,以及肾脏和小肠中的多个白色至黄色结节。组织学上,这些器官被排列成片状的肿瘤性小淋巴细胞部分或完全消除,有中度的异细胞增多和异核细胞增多,有丝分裂计数低。这些细胞对CD79α和CD20进行了广泛的免疫标记,并且对CD3呈阴性。多中心B-SL的诊断已经确立,据作者所知,它以前从未在该属中描述过。
{"title":"B-cell small lymphocytic lymphoma in a free-ranging South American fur seal (Arctocephalus australis).","authors":"Derek B de Amorim,&nbsp;Paula R Ribeiro,&nbsp;Marcele B Bandinelli,&nbsp;Joanna V Z Echenique,&nbsp;Matheus V Bianchi,&nbsp;Paula R Almeida,&nbsp;Fernando R Spilki,&nbsp;Leticia F Baumbach,&nbsp;Luciana Sonne","doi":"10.3354/dao03757","DOIUrl":"10.3354/dao03757","url":null,"abstract":"<p><p>Lymphomas are malignant neoplasms of the hematopoietic system arising from lymphocytes with highly variable biologic behavior. B-cell small lymphocytic lymphoma (B-SLL) is a non-Hodgkin lymphoma infrequently described in domestic and wild animals. The present study describes a case of B-SLL in a free-ranging adult male Arctocephalus australis in Brazil. The main necropsy findings included poor body condition, generalized lymphadenomegaly, severe and diffuse splenomegaly, and multiple, white to yellow nodules in the kidneys and small intestine. Histologically, these organs were partially or totally effaced by neoplastic small lymphocytes arranged in sheets, with moderate anisocytosis and anisokaryosis and a low mitotic count. These cells diffusely immunolabeled for CD79α and CD20, and were negative for CD3. A diagnosis of multicentric B-SLL was established and to the authors' knowledge, it has not been previously described in this genus.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"156 ","pages":"1-6"},"PeriodicalIF":1.4,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41194444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Decline of Marteilia cochillia in Ría de Arousa may be due to increased resistance in host Cerastoderma edule. Arousaía地区的科氏马氏体的衰退可能是由于寄主Cerastoderma edule的抗性增加。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-10-12 DOI: 10.3354/dao03756
David Iglesias, Asunción Cao, María J Carballal, Antonio Villalba

A huge, unprecedented mortality of cockle Cerastoderma edule caused by the protist Marteilia cochillia, which had never before been detected in Galicia (NW Spain), brought on a cockle fishery collapse in the Ría de Arousa (Galicia) in 2012. Since then, the disease dynamic pattern in the shellfish bed of Lombos do Ulla (at the inner area of that ria) involved an overwhelming annual wave of infections and subsequent cockle mass mortality that caused the near extinction of every cohort recruited to that bed. However, a pattern shift was detected among wild cohorts recruiting since 2016, with progressive declines of marteiliosis prevalence and increments in cockle survival. This suggested 2 non-exclusive hypotheses: increasing marteiliosis resistance through natural selection, and reduced abundance and/or virulence of the parasite. A field experiment was performed to assess these hypotheses by comparing marteiliosis prevalence and severity, as well as mortality, in cockles that naturally recruited to this bed in 2017 and 2018 with those of naïve cockles collected from a marteiliosis-free area and transplanted into Lombos do Ulla in 2017 and 2018. Marteiliosis prevalence and cumulative cockle mortality quickly reached very high values among the transplanted cockles, demonstrating that the parasite remained present and virulent in the area. Conversely, marteiliosis prevalence and cockle mortality were much lower in the cockles that recruited to Lombos do Ulla, suggesting increased resistance that may have been driven by natural selection. The young age at which cockles start reproduction and the very high mortality caused by marteiliosis may have enhanced natural selection.

2012年,在加利西亚(西班牙西北部)从未发现过由原生动物科氏马氏体(Marteilia cocchilia)引起的一场前所未有的巨大的死鸡场,导致了阿罗萨河(加利西亚)的一场鸡场渔业崩溃。从那时起,Lombos do Ulla贝类养殖场(位于该养殖场的内部区域)的疾病动态模式涉及每年压倒性的感染浪潮和随后的鸡群大规模死亡,导致该养殖场招募的每一个群体几乎灭绝。然而,自2016年以来,在招募的野生种群中发现了一种模式转变,马氏体病患病率逐渐下降,公鸡存活率增加。这提出了两个非排他性的假设:通过自然选择增加对马氏体的耐药性,降低寄生虫的丰度和/或毒力。为了评估这些假设,进行了一项实地实验,将2017年和2018年自然招募到该床的公鸡的马氏体病患病率、严重程度以及死亡率与2017年和18年从无马氏体病地区采集并移植到乌拉龙目的天真公鸡进行了比较。在移植的公鸡中,马氏体病的流行率和累积的公鸡死亡率很快达到了非常高的值,这表明这种寄生虫在该地区仍然存在并具有毒性。相反,在被招募到Lombos do Ulla的公鸡中,马氏体病的患病率和公鸡死亡率要低得多,这表明抵抗力的增加可能是由自然选择驱动的。公鸡开始繁殖的年龄很小,并且由马氏体病引起的死亡率很高,这可能增强了自然选择。
{"title":"Decline of Marteilia cochillia in Ría de Arousa may be due to increased resistance in host Cerastoderma edule.","authors":"David Iglesias,&nbsp;Asunción Cao,&nbsp;María J Carballal,&nbsp;Antonio Villalba","doi":"10.3354/dao03756","DOIUrl":"10.3354/dao03756","url":null,"abstract":"<p><p>A huge, unprecedented mortality of cockle Cerastoderma edule caused by the protist Marteilia cochillia, which had never before been detected in Galicia (NW Spain), brought on a cockle fishery collapse in the Ría de Arousa (Galicia) in 2012. Since then, the disease dynamic pattern in the shellfish bed of Lombos do Ulla (at the inner area of that ria) involved an overwhelming annual wave of infections and subsequent cockle mass mortality that caused the near extinction of every cohort recruited to that bed. However, a pattern shift was detected among wild cohorts recruiting since 2016, with progressive declines of marteiliosis prevalence and increments in cockle survival. This suggested 2 non-exclusive hypotheses: increasing marteiliosis resistance through natural selection, and reduced abundance and/or virulence of the parasite. A field experiment was performed to assess these hypotheses by comparing marteiliosis prevalence and severity, as well as mortality, in cockles that naturally recruited to this bed in 2017 and 2018 with those of naïve cockles collected from a marteiliosis-free area and transplanted into Lombos do Ulla in 2017 and 2018. Marteiliosis prevalence and cumulative cockle mortality quickly reached very high values among the transplanted cockles, demonstrating that the parasite remained present and virulent in the area. Conversely, marteiliosis prevalence and cockle mortality were much lower in the cockles that recruited to Lombos do Ulla, suggesting increased resistance that may have been driven by natural selection. The young age at which cockles start reproduction and the very high mortality caused by marteiliosis may have enhanced natural selection.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"156 ","pages":"7-13"},"PeriodicalIF":1.4,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41194445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Helicobacter delphinicola infection and the risk of gastric disease in common bottlenose dolphin. 常见宽吻海豚幽门螺杆菌感染与胃病风险。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-09-28 DOI: 10.3354/dao03751
Takao Segawa, Yoshito Ohno, Masanori Kurita, Toshiaki Ishibashi, Motoi Yoshioka

Gastritis and gastric ulcers are well-recognized conditions in cetaceans; bacteria of the genus Helicobacter are considered the primary cause of these diseases. Dolphins have been shown to be susceptible to infection by at least 2 gastric species of Helicobacter, H. cetorum and H. delphinicola, both of which are closely related to the human pathogen H. pylori. In the present study, we evaluated the carriage rate and relationship to gastric disease of H. cetorum and H. delphinicola, based on a study population of 82 dolphins maintained at 21 facilities in Japan. Of these 82 dolphins, 79 (96.3%) and 45 (54.9%) were positive for H. cetorum and H. delphinicola, respectively; H. delphinicola infection was significantly associated with chronic gastric diseases (odds rate: 5.9; 95% CI: 2.1-16.9), but no such association was detected for H. cetorum. Of the 21 facilities, 20 (95%) and 11 (55%) housed H. cetorum- and H. delphinicola-positive dolphins, respectively, and our study suggested that the transmission between dolphins occurs quickly within pools. These findings indicate that methods will need to be established to prevent the transmission of Helicobacter infections within facilities housing dolphins.

胃炎和胃溃疡是鲸目动物公认的疾病;幽门螺杆菌属细菌被认为是这些疾病的主要原因。海豚已被证明易受至少两种胃幽门螺杆菌感染,这两种幽门螺杆菌都与人类病原体幽门螺杆菌密切相关。在本研究中,我们基于在日本21个设施中饲养的82只海豚的研究种群,评估了海燕和飞燕的携带率及其与胃病的关系。在这82只海豚中,分别有79只(96.3%)和45只(54.9%)对鲸豚和飞燕草呈阳性;飞燕草感染与慢性胃部疾病显著相关(优势率:5.9;95%可信区间:2.1-16.9),但在鹅膏菌中未检测到这种关联。在21个设施中,分别有20个(95%)和11个(55%)容纳了阳性海豚,我们的研究表明,海豚之间的传播在水池中发生得很快。这些发现表明,需要建立方法来防止海豚饲养设施内幽门螺杆菌感染的传播。
{"title":"Helicobacter delphinicola infection and the risk of gastric disease in common bottlenose dolphin.","authors":"Takao Segawa,&nbsp;Yoshito Ohno,&nbsp;Masanori Kurita,&nbsp;Toshiaki Ishibashi,&nbsp;Motoi Yoshioka","doi":"10.3354/dao03751","DOIUrl":"https://doi.org/10.3354/dao03751","url":null,"abstract":"<p><p>Gastritis and gastric ulcers are well-recognized conditions in cetaceans; bacteria of the genus Helicobacter are considered the primary cause of these diseases. Dolphins have been shown to be susceptible to infection by at least 2 gastric species of Helicobacter, H. cetorum and H. delphinicola, both of which are closely related to the human pathogen H. pylori. In the present study, we evaluated the carriage rate and relationship to gastric disease of H. cetorum and H. delphinicola, based on a study population of 82 dolphins maintained at 21 facilities in Japan. Of these 82 dolphins, 79 (96.3%) and 45 (54.9%) were positive for H. cetorum and H. delphinicola, respectively; H. delphinicola infection was significantly associated with chronic gastric diseases (odds rate: 5.9; 95% CI: 2.1-16.9), but no such association was detected for H. cetorum. Of the 21 facilities, 20 (95%) and 11 (55%) housed H. cetorum- and H. delphinicola-positive dolphins, respectively, and our study suggested that the transmission between dolphins occurs quickly within pools. These findings indicate that methods will need to be established to prevent the transmission of Helicobacter infections within facilities housing dolphins.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"155 ","pages":"187-192"},"PeriodicalIF":1.4,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41103876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Perkinsus olseni in green-lipped mussels Perna canaliculus: diagnostic evaluation, prevalence, and distribution. 绿唇贻贝Perna Canalitulus中的Perksus olseni:诊断评估、患病率和分布。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-09-28 DOI: 10.3354/dao03750
Henry S Lane, Diana Jaramillo, Mukul Sharma

Perkinsus olseni (Perkinsidae) is a molluscan parasite notifiable to the World Organisation for Animal Health that is reported in several shellfish hosts in New Zealand, including the native green-lipped mussel Perna canaliculus. Green-lipped mussels comprise over half of New Zealand's aquaculture export value and have historically been considered free of serious diseases based on extensive histology-based surveillance. The discovery of P. olseni in green-lipped mussels has raised questions about future disease threats to green-lipped mussels, particularly under changing ocean climatic conditions. Using mussels collected from farmed (n = 358) and wild (n = 236) populations, we aimed to determine the distribution and prevalence of P. olseni in green-lipped mussels around New Zealand, and assess the performance of diagnostic tests, including real-time PCR, conventional PCR, and culture using Ray's fluid thioglycolate medium (RFTM). Prevalence and diagnostic test performance was evaluated using Bayesian latent class analysis with informative priors. The prevalence of P. olseni was 0-3%, except for 1 wild population from a harbour where prevalence was 22%. Real-time PCR had the highest diagnostic sensitivity (87%) compared to 62 and 21% for conventional PCR and RFTM, respectively. Diagnostic specificity was similar among all methods (96-98%). No mortality was observed during the study. Our results suggest that real-time PCR is the diagnostic test best suited for surveillance of P. olseni in subclinically infected green-lipped mussels under New Zealand conditions.

Perkinsus olseni(Perkinsidae)是一种软体动物寄生虫,应向世界动物卫生组织报告,据报道,它存在于新西兰的几种贝类宿主中,包括本地的绿唇贻贝Perna canaliculus。绿唇贻贝占新西兰水产养殖出口价值的一半以上,根据广泛的组织学监测,历史上一直被认为没有严重疾病。在绿唇贻贝中发现的P.olseni引发了人们对绿唇贻蚌未来疾病威胁的质疑,特别是在不断变化的海洋气候条件下。使用从养殖(n=358)和野生(n=236)种群中采集的贻贝,我们旨在确定新西兰各地绿唇贻贝中奥氏假单胞菌的分布和流行率,并评估诊断测试的性能,包括实时PCR、常规PCR和使用Ray液体巯基乙酸培养基(RFTM)培养。使用具有信息先验的贝叶斯潜在类别分析来评估患病率和诊断测试性能。除了1个来自港口的野生种群的患病率为22%外,奥氏疟原虫的患病率是0-3%。实时PCR的诊断灵敏度最高(87%),而常规PCR和RFTM分别为62%和21%。所有方法的诊断特异性相似(96-98%)。研究期间未观察到死亡。我们的研究结果表明,实时PCR是最适合在新西兰条件下监测亚临床感染的绿唇贻贝中的奥氏假单胞菌的诊断测试。
{"title":"Perkinsus olseni in green-lipped mussels Perna canaliculus: diagnostic evaluation, prevalence, and distribution.","authors":"Henry S Lane,&nbsp;Diana Jaramillo,&nbsp;Mukul Sharma","doi":"10.3354/dao03750","DOIUrl":"https://doi.org/10.3354/dao03750","url":null,"abstract":"<p><p>Perkinsus olseni (Perkinsidae) is a molluscan parasite notifiable to the World Organisation for Animal Health that is reported in several shellfish hosts in New Zealand, including the native green-lipped mussel Perna canaliculus. Green-lipped mussels comprise over half of New Zealand's aquaculture export value and have historically been considered free of serious diseases based on extensive histology-based surveillance. The discovery of P. olseni in green-lipped mussels has raised questions about future disease threats to green-lipped mussels, particularly under changing ocean climatic conditions. Using mussels collected from farmed (n = 358) and wild (n = 236) populations, we aimed to determine the distribution and prevalence of P. olseni in green-lipped mussels around New Zealand, and assess the performance of diagnostic tests, including real-time PCR, conventional PCR, and culture using Ray's fluid thioglycolate medium (RFTM). Prevalence and diagnostic test performance was evaluated using Bayesian latent class analysis with informative priors. The prevalence of P. olseni was 0-3%, except for 1 wild population from a harbour where prevalence was 22%. Real-time PCR had the highest diagnostic sensitivity (87%) compared to 62 and 21% for conventional PCR and RFTM, respectively. Diagnostic specificity was similar among all methods (96-98%). No mortality was observed during the study. Our results suggest that real-time PCR is the diagnostic test best suited for surveillance of P. olseni in subclinically infected green-lipped mussels under New Zealand conditions.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"155 ","pages":"175-185"},"PeriodicalIF":1.4,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41116646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pseudacris regilla metamorphs acquire resistance to Batrachochytrium dendrobatidis after exposure to the killed fungus. 在暴露于被杀死的真菌后,Pseudacris regilla变态获得了对树状蜡染虫的抗性。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-09-28 DOI: 10.3354/dao03753
Taegan A McMahon, Caitlin L Nordheim, Sarah E Detmering, Pieter T J Johnson, Jason R Rohr, David J Civitello

The pathogenic fungus Batrachochytrium dendrobatidis (Bd) is associated with drastic global amphibian declines. Prophylactic exposure to killed zoospores and the soluble chemicals they produce (Bd metabolites) can induce acquired resistance to Bd in adult Cuban treefrogs Osteopilus septentrionalis. Here, we exposed metamorphic frogs of a second species, the Pacific chorus frog Pseudacris regilla, to one of 2 prophylactic treatments prior to live Bd exposures: killed Bd zoospores with metabolites, killed zoospores alone, or a water control. Prior exposure to killed Bd zoospores with metabolites reduced Bd infection intensity in metamorphic Pacific chorus frogs by 60.4% compared to control frogs. Interestingly, Bd intensity in metamorphs previously exposed to killed zoospores alone did not differ in magnitude relative to the control metamorphs, nor to those treated with killed zoospores plus metabolites. Previous work indicated that Bd metabolites alone can induce acquired resistance in tadpoles, and so these findings together indicate that it is possible that the soluble Bd metabolites may contain immunomodulatory components that drive this resistance phenotype. Our results expand the generality of this prophylaxis work by identifying a second amphibian species (Pacific chorus frog) and an additional amphibian life stage (metamorphic frog) that can acquire resistance to Bd after metabolite exposure. This work increases hopes that a Bd-metabolite prophylaxis might be widely effective across amphibian species and life stages.

致病性真菌树状蝙蝠(Batrachochytrium dendrobatidis,Bd)与全球两栖动物数量急剧下降有关。预防性暴露于杀死的游动孢子及其产生的可溶性化学物质(Bd代谢产物)可以诱导成年古巴树蛙对Bd的获得性抗性。在这里,我们让第二个物种的变质蛙,太平洋合唱蛙Pseudacris regilla,在活的Bd暴露之前接受两种预防性治疗之一:用代谢产物杀死Bd游动孢子,单独杀死游动孢子,或水控制。与对照蛙相比,先前暴露于含有代谢物的杀死的Bd游动孢子可使变质太平洋合唱蛙的Bd感染强度降低60.4%。有趣的是,以前单独暴露于杀死的游动孢子的变质物的Bd强度与对照变质物的大小没有差异,也与用杀死的游动芽孢和代谢物处理的变质物没有差异。先前的研究表明,单独的Bd代谢产物可以诱导蝌蚪的获得性抗性,因此这些发现共同表明,可溶性Bd代谢物可能含有驱动这种抗性表型的免疫调节成分。我们的研究结果通过鉴定第二种两栖动物物种(太平洋合唱蛙)和一个额外的两栖动物生命阶段(变质蛙)来扩大这项预防工作的普遍性,这些两栖动物在代谢产物暴露后可以获得对Bd的抗性。这项工作增加了人们对Bd代谢产物预防可能在两栖动物物种和生命阶段广泛有效的希望。
{"title":"Pseudacris regilla metamorphs acquire resistance to Batrachochytrium dendrobatidis after exposure to the killed fungus.","authors":"Taegan A McMahon,&nbsp;Caitlin L Nordheim,&nbsp;Sarah E Detmering,&nbsp;Pieter T J Johnson,&nbsp;Jason R Rohr,&nbsp;David J Civitello","doi":"10.3354/dao03753","DOIUrl":"https://doi.org/10.3354/dao03753","url":null,"abstract":"<p><p>The pathogenic fungus Batrachochytrium dendrobatidis (Bd) is associated with drastic global amphibian declines. Prophylactic exposure to killed zoospores and the soluble chemicals they produce (Bd metabolites) can induce acquired resistance to Bd in adult Cuban treefrogs Osteopilus septentrionalis. Here, we exposed metamorphic frogs of a second species, the Pacific chorus frog Pseudacris regilla, to one of 2 prophylactic treatments prior to live Bd exposures: killed Bd zoospores with metabolites, killed zoospores alone, or a water control. Prior exposure to killed Bd zoospores with metabolites reduced Bd infection intensity in metamorphic Pacific chorus frogs by 60.4% compared to control frogs. Interestingly, Bd intensity in metamorphs previously exposed to killed zoospores alone did not differ in magnitude relative to the control metamorphs, nor to those treated with killed zoospores plus metabolites. Previous work indicated that Bd metabolites alone can induce acquired resistance in tadpoles, and so these findings together indicate that it is possible that the soluble Bd metabolites may contain immunomodulatory components that drive this resistance phenotype. Our results expand the generality of this prophylaxis work by identifying a second amphibian species (Pacific chorus frog) and an additional amphibian life stage (metamorphic frog) that can acquire resistance to Bd after metabolite exposure. This work increases hopes that a Bd-metabolite prophylaxis might be widely effective across amphibian species and life stages.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"155 ","pages":"193-198"},"PeriodicalIF":1.4,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41131082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of methods for determining Batrachochytrium dendrobatidis zoospore viability. 水蛭壶菌游动孢子活力测定方法的比较。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-09-14 DOI: 10.3354/dao03749
Adeline Chew, Matt West, Laura A Brannelly

The emerging fungal pathogen Batrachochytrium dendrobatidis (Bd) threatens hundreds of amphibian species globally. During laboratory-based experiments it is often essential to quantify live Bd cells, but a comparison of the effectiveness of methods for counting and assessing the viability of the infectious zoospore life stage has not been done. A direct comparison of staining methods that assess viability will ensure that the most accurate and efficient method is used. Here, we compared the use of 2 relatively cheap common stains, trypan blue and methylene blue, and assessed their accuracy and precision for estimating the viability of Bd zoospores during both manual counting and colorimetric assays. We stained known proportions of killed Bd zoospores (0, 0.25, 0.50, 0.75, and 1.00) with each stain and estimated the proportion of stained (dead) and unstained (viable) cells in each sample using both manual counting and colorimetric assays. Trypan blue was found to be a much more effective stain than methylene blue for both microscopy and colorimetric assays. Additionally, counting zoospores via microscopy was both a more accurate and precise technique. We recommend using manual counts via microscopy using the trypan blue stain for assessing Bd zoospore viability.

新型真菌病原体水蛭壶菌(Batrachochytrium dendroatidis, Bd)威胁着全球数百种两栖动物。在以实验室为基础的实验中,通常必须对活的Bd细胞进行量化,但对计数和评估感染性游动孢子生命阶段活力的方法的有效性进行比较尚未完成。直接比较评估细胞活力的染色方法将确保使用最准确和有效的方法。在这里,我们比较了两种相对便宜的常用染色剂,台盼蓝和亚甲基蓝,并评估了它们在人工计数和比色测定中估计Bd游动孢子活力的准确性和精密度。我们用每种染色剂对已知比例的bdzoo孢子(0、0.25、0.50、0.75和1.00)进行染色,并使用人工计数和比色法估计每个样品中染色(死亡)和未染色(活)细胞的比例。台盼蓝被发现是比亚甲基蓝更有效的染色显微镜和比色测定。此外,通过显微镜计算游动孢子是一种更准确和精确的技术。我们建议使用人工计数,通过显微镜使用台盼蓝染色来评估Bd游动孢子的活力。
{"title":"Comparison of methods for determining Batrachochytrium dendrobatidis zoospore viability.","authors":"Adeline Chew,&nbsp;Matt West,&nbsp;Laura A Brannelly","doi":"10.3354/dao03749","DOIUrl":"https://doi.org/10.3354/dao03749","url":null,"abstract":"<p><p>The emerging fungal pathogen Batrachochytrium dendrobatidis (Bd) threatens hundreds of amphibian species globally. During laboratory-based experiments it is often essential to quantify live Bd cells, but a comparison of the effectiveness of methods for counting and assessing the viability of the infectious zoospore life stage has not been done. A direct comparison of staining methods that assess viability will ensure that the most accurate and efficient method is used. Here, we compared the use of 2 relatively cheap common stains, trypan blue and methylene blue, and assessed their accuracy and precision for estimating the viability of Bd zoospores during both manual counting and colorimetric assays. We stained known proportions of killed Bd zoospores (0, 0.25, 0.50, 0.75, and 1.00) with each stain and estimated the proportion of stained (dead) and unstained (viable) cells in each sample using both manual counting and colorimetric assays. Trypan blue was found to be a much more effective stain than methylene blue for both microscopy and colorimetric assays. Additionally, counting zoospores via microscopy was both a more accurate and precise technique. We recommend using manual counts via microscopy using the trypan blue stain for assessing Bd zoospore viability.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"155 ","pages":"141-146"},"PeriodicalIF":1.4,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Infestation rates of lice Lepeophtheirus salmonis and Caligus elongatus on Atlantic salmon in fixed and towed sentinel cages. 固定式和拖曳式哨兵笼中大西洋大鲑鱼受鲑蛉和长尾大蠊的侵害率。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-09-14 DOI: 10.3354/dao03748
C C Pert, I S Wallace, P MacDonald, S C Ives, A G Murray, B Rabe

Sea lice are a key limitation to sustainable salmon aquaculture, and effective monitoring strategies are critical for the management of these parasites. Sentinel cages are an established means of assessing infestation pressure at fixed locations, but as smolts move through systems they will be exposed to varying lice densities. As a means of assessing infestation pressure along trajectories, we describe the development and application of towed sentinel cages (TSCs) in a Scottish sea loch containing salmonid aquaculture. Trial deployments took place over 3 yr (2016-2018), and levels of sea lice infestation were compared between methodologies. Oceanographic data was collected alongside TSCs to put the results into the environmental context that smolts and sea lice experienced during the tows. The sea lice infestation rates found from TSCs were comparable to those on contemporaneously deployed fixed sentinel cages. Thus, due to their practicability and consistency with other surveillance methods, TSCs could be used to improve the assessment of exposure risk along wild salmonid smolt migration trajectories, where these are known.

海虱是鲑鱼可持续养殖的主要限制因素,有效的监测策略对这些寄生虫的管理至关重要。哨兵笼是在固定地点评估虫害压力的一种既定手段,但随着幼崽在系统中移动,它们将暴露在不同的虱子密度中。作为沿轨迹评估虫害压力的一种手段,我们描述了拖曳哨笼(TSCs)在苏格兰一个含有鲑科水产养殖的海湖中的发展和应用。试验部署进行了3年(2016-2018),并比较了不同方法之间的海虱感染水平。海洋学数据与tsc一起收集,将结果放入拖带期间小蝌蚪和海虱经历的环境背景中。从tsc中发现的海虱感染率与同期部署的固定哨兵笼相当。因此,由于tsc的实用性和与其他监测方法的一致性,tsc可用于改善已知野生鲑鱼幼鱼迁移轨迹沿线暴露风险的评估。
{"title":"Infestation rates of lice Lepeophtheirus salmonis and Caligus elongatus on Atlantic salmon in fixed and towed sentinel cages.","authors":"C C Pert,&nbsp;I S Wallace,&nbsp;P MacDonald,&nbsp;S C Ives,&nbsp;A G Murray,&nbsp;B Rabe","doi":"10.3354/dao03748","DOIUrl":"https://doi.org/10.3354/dao03748","url":null,"abstract":"<p><p>Sea lice are a key limitation to sustainable salmon aquaculture, and effective monitoring strategies are critical for the management of these parasites. Sentinel cages are an established means of assessing infestation pressure at fixed locations, but as smolts move through systems they will be exposed to varying lice densities. As a means of assessing infestation pressure along trajectories, we describe the development and application of towed sentinel cages (TSCs) in a Scottish sea loch containing salmonid aquaculture. Trial deployments took place over 3 yr (2016-2018), and levels of sea lice infestation were compared between methodologies. Oceanographic data was collected alongside TSCs to put the results into the environmental context that smolts and sea lice experienced during the tows. The sea lice infestation rates found from TSCs were comparable to those on contemporaneously deployed fixed sentinel cages. Thus, due to their practicability and consistency with other surveillance methods, TSCs could be used to improve the assessment of exposure risk along wild salmonid smolt migration trajectories, where these are known.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"155 ","pages":"165-174"},"PeriodicalIF":1.4,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10233113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Historical isolates of dolphin morbillivirus: origin and new data. 海豚麻疹病毒的历史分离株:起源和新资料。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-09-14 DOI: 10.3354/dao03752
Marie-Françoise Van Bressem, Juan Antonio Raga, Mariano Domingo, Pádraig Duignan

Dolphin morbillivirus (DMV) was isolated in striped dolphins Stenella coeruleoalba from the Mediterranean Sea stranded along the coast of Spain during a lethal epidemic that killed thousands of individuals in 1990-1992. Though some of these isolates (MUC, 16A and the reference strain) have been extensively characterised, details on their origin were not reported in the literature, and records for these isolates are often difficult to trace and are, sometimes, erroneous. Here, we provide unpublished biological and histopathological data for these isolates, summarize the literature on their characterization and make suggestions for future studies.

海豚麻疹病毒(DMV)是从地中海的条纹海豚中分离出来的,这些条纹海豚在1990-1992年的致命流行病中被困在西班牙海岸,造成数千人死亡。虽然这些分离株(MUC, 16A和参考菌株)已被广泛表征,但其来源的细节未在文献中报道,并且这些分离株的记录通常难以追踪,有时甚至是错误的。在此,我们提供了未发表的这些分离株的生物学和组织病理学数据,总结了它们的表征文献,并对未来的研究提出了建议。
{"title":"Historical isolates of dolphin morbillivirus: origin and new data.","authors":"Marie-Françoise Van Bressem,&nbsp;Juan Antonio Raga,&nbsp;Mariano Domingo,&nbsp;Pádraig Duignan","doi":"10.3354/dao03752","DOIUrl":"https://doi.org/10.3354/dao03752","url":null,"abstract":"<p><p>Dolphin morbillivirus (DMV) was isolated in striped dolphins Stenella coeruleoalba from the Mediterranean Sea stranded along the coast of Spain during a lethal epidemic that killed thousands of individuals in 1990-1992. Though some of these isolates (MUC, 16A and the reference strain) have been extensively characterised, details on their origin were not reported in the literature, and records for these isolates are often difficult to trace and are, sometimes, erroneous. Here, we provide unpublished biological and histopathological data for these isolates, summarize the literature on their characterization and make suggestions for future studies.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"155 ","pages":"159-163"},"PeriodicalIF":1.4,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temperature-associated virulence, species susceptibility and interspecies transmission of a Lactococcus petauri strain from rainbow trout. 虹鳟鱼petauri乳球菌菌株的温度相关毒力、物种易感性和种间传播。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-09-14 DOI: 10.3354/dao03747
Eric Maxwell Littman, Taylor I Heckman, Zeinab Yazdi, Tresa Veek, Kaveramma Mukkatira, Mark Adkison, Ashley Powell, Alvin Camus, Esteban Soto

Lactococcus petauri is an important emergent aquaculture pathogen in the USA. To better understand environmental conditions conducive to piscine lactococcosis and the susceptibility of fish species, laboratory-controlled challenges were used as models of infection. Rainbow trout Oncorhynchus mykiss maintained at 13 or 18°C were challenged by intracoelomic (ICe) injection with 101, 103 or 105 colony-forming units per fish (CFU fish-1) and monitored for 21 d. At 13°C, trout experienced mortalities of 7, 7 and 0%, and bacterial persistence of 0, 20 and 0% in survivors, respectively. When exposed to the same bacterial doses, trout maintained at 18°C experienced mortalities of 59, 84 and 91%, and bacterial persistence of 60, 66 and 0% in survivors, confirming a significant role of temperature in the pathogenesis of lactococcosis. Additionally, the susceptibility of rainbow trout, Chinook salmon Oncorhynchus tshawytscha, white sturgeon Acipenser transmontanus, Nile tilapia Oreochromis niloticus, and koi Cyprinus carpio to infection by L. petauri was compared using ICe challenges at 18°C. Trout and salmon experienced 96 and 56% cumulative mortality, respectively, and 17% of surviving salmon remained persistently infected. There were no mortalities in the other fish species, and no culturable bacteria recovered at the end of the challenge. However, when surviving fish were used in further cohabitation trials, naïve trout housed with previously exposed tilapia exhibited 6% mortality, demonstrating that non-salmonids can become sub-clinical carriers of this pathogen. The data obtained provide useful information regarding temperature-associated virulence, fish species susceptibility, and potential carrier transmission of L. petauri that can be used in the development of better management practices to protect against piscine lactococcosis.

petauri乳球菌是美国重要的新兴水产养殖病原体。为了更好地了解有利于鱼乳球菌病的环境条件和鱼类的易感性,采用实验室控制的挑战作为感染模型。将维持在13或18°C的虹鳟(Oncorhynchus mykiss)进行卵腔内(ICe)注射,每条鱼有101、103或105个菌落形成单位(CFU鱼-1),并监测21 d。在13°C时,鳟鱼的死亡率分别为7%、7%和0%,幸存者的细菌持久性分别为0%、20%和0%。当暴露于相同细菌剂量时,保持在18°C的鳟鱼的死亡率分别为59%、84%和91%,幸存者的细菌持久性分别为60%、66%和0%,证实了温度在乳球菌病发病机制中的重要作用。此外,在18°C的冰激淋条件下,比较了虹鳟鱼、奇努克鲑鱼Oncorhynchus tshawytscha、白鲟acpenser transmontanus、尼罗罗非鱼Oreochromis niloticus和鲤鱼鲤对L. petauri感染的敏感性。鳟鱼和鲑鱼的累计死亡率分别为96%和56%,存活的鲑鱼中有17%仍然持续感染。其他鱼类没有死亡,在挑战结束时没有可培养的细菌恢复。然而,当将存活的鱼用于进一步的同居试验时,naïve鳟鱼与先前暴露的罗非鱼一起饲养,显示出6%的死亡率,表明非鲑科鱼类可以成为这种病原体的亚临床携带者。获得的数据提供了有关温度相关毒力、鱼类易感性和潜在载体传播的有用信息,可用于制定更好的管理措施,以防止鱼类乳球菌病。
{"title":"Temperature-associated virulence, species susceptibility and interspecies transmission of a Lactococcus petauri strain from rainbow trout.","authors":"Eric Maxwell Littman,&nbsp;Taylor I Heckman,&nbsp;Zeinab Yazdi,&nbsp;Tresa Veek,&nbsp;Kaveramma Mukkatira,&nbsp;Mark Adkison,&nbsp;Ashley Powell,&nbsp;Alvin Camus,&nbsp;Esteban Soto","doi":"10.3354/dao03747","DOIUrl":"https://doi.org/10.3354/dao03747","url":null,"abstract":"<p><p>Lactococcus petauri is an important emergent aquaculture pathogen in the USA. To better understand environmental conditions conducive to piscine lactococcosis and the susceptibility of fish species, laboratory-controlled challenges were used as models of infection. Rainbow trout Oncorhynchus mykiss maintained at 13 or 18°C were challenged by intracoelomic (ICe) injection with 101, 103 or 105 colony-forming units per fish (CFU fish-1) and monitored for 21 d. At 13°C, trout experienced mortalities of 7, 7 and 0%, and bacterial persistence of 0, 20 and 0% in survivors, respectively. When exposed to the same bacterial doses, trout maintained at 18°C experienced mortalities of 59, 84 and 91%, and bacterial persistence of 60, 66 and 0% in survivors, confirming a significant role of temperature in the pathogenesis of lactococcosis. Additionally, the susceptibility of rainbow trout, Chinook salmon Oncorhynchus tshawytscha, white sturgeon Acipenser transmontanus, Nile tilapia Oreochromis niloticus, and koi Cyprinus carpio to infection by L. petauri was compared using ICe challenges at 18°C. Trout and salmon experienced 96 and 56% cumulative mortality, respectively, and 17% of surviving salmon remained persistently infected. There were no mortalities in the other fish species, and no culturable bacteria recovered at the end of the challenge. However, when surviving fish were used in further cohabitation trials, naïve trout housed with previously exposed tilapia exhibited 6% mortality, demonstrating that non-salmonids can become sub-clinical carriers of this pathogen. The data obtained provide useful information regarding temperature-associated virulence, fish species susceptibility, and potential carrier transmission of L. petauri that can be used in the development of better management practices to protect against piscine lactococcosis.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"155 ","pages":"147-158"},"PeriodicalIF":1.4,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10240207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluating short- to medium-term effects of implantable satellite tags on southern right whales Eubalaena australis. 评估植入式卫星标签对南露脊鲸的中短期影响。
IF 1.4 4区 农林科学 Q3 FISHERIES Pub Date : 2023-09-14 DOI: 10.3354/dao03730
Claire Charlton, Fredrik Christiansen, Rhianne Ward, Alice I Mackay, Virginia Andrews-Goff, Alexandre N Zerbini, Simon Childerhouse, Sacha Guggenheimer, Bridgette O'Shannessy, Robert L Brownell

Improving our understanding of the effects of satellite tags on large whales is a critical step in ongoing tag development to minimise potential health effects whilst addressing important research questions that enhance conservation management policy. In 2014, satellite tags were deployed on 9 female southern right whales Eubalaena australis accompanied by a calf off Australia. Photo-identification resights (n = 48) of 4 photo-identified individuals were recorded 1 to 2894 d (1-8 yr) post-tagging. Short-term (<22 d) effects observed included localised and regional swelling, depression at the tag site, blubber extrusion, skin loss and pigmentation colour change. Broad swelling observable from lateral but not aerial imagery (~1.2 m diameter or ~9% of body length) and depression at the tag site persisted up to 1446 d post-tagging for 1 individual, indicating a persistent foreign-body response or infection. Two tagged individuals returned 4 yr post-tagging in 2018 with a calf, and the medium-term effects were evaluated by comparing body condition of tagged whales with non-tagged whales. These females calved in a typical 4 yr interval, suggesting no apparent immediate impact of tagging on reproduction for these individuals, but longer-term monitoring is needed. There was no observable difference in the body condition between the 2 tagged and non-tagged females. Ongoing monitoring post-tagging is required to build on the sample size and statistical power. We demonstrate the value of long-term monitoring programmes and a collaborative approach for evaluating effects from satellite-tagging cetaceans to support species management.

提高我们对卫星标签对大型鲸鱼的影响的理解是正在进行的标签开发的关键一步,以尽量减少潜在的健康影响,同时解决加强保护管理政策的重要研究问题。2014年,在澳大利亚海域,9头雌性南露脊鲸(Eubalaena australis)和一头幼鲸被部署了卫星标签。在标记后1 ~ 2894 d (1 ~ 8 yr)记录4只照片识别个体的照片识别观察(n = 48)。短期(
{"title":"Evaluating short- to medium-term effects of implantable satellite tags on southern right whales Eubalaena australis.","authors":"Claire Charlton,&nbsp;Fredrik Christiansen,&nbsp;Rhianne Ward,&nbsp;Alice I Mackay,&nbsp;Virginia Andrews-Goff,&nbsp;Alexandre N Zerbini,&nbsp;Simon Childerhouse,&nbsp;Sacha Guggenheimer,&nbsp;Bridgette O'Shannessy,&nbsp;Robert L Brownell","doi":"10.3354/dao03730","DOIUrl":"https://doi.org/10.3354/dao03730","url":null,"abstract":"<p><p>Improving our understanding of the effects of satellite tags on large whales is a critical step in ongoing tag development to minimise potential health effects whilst addressing important research questions that enhance conservation management policy. In 2014, satellite tags were deployed on 9 female southern right whales Eubalaena australis accompanied by a calf off Australia. Photo-identification resights (n = 48) of 4 photo-identified individuals were recorded 1 to 2894 d (1-8 yr) post-tagging. Short-term (<22 d) effects observed included localised and regional swelling, depression at the tag site, blubber extrusion, skin loss and pigmentation colour change. Broad swelling observable from lateral but not aerial imagery (~1.2 m diameter or ~9% of body length) and depression at the tag site persisted up to 1446 d post-tagging for 1 individual, indicating a persistent foreign-body response or infection. Two tagged individuals returned 4 yr post-tagging in 2018 with a calf, and the medium-term effects were evaluated by comparing body condition of tagged whales with non-tagged whales. These females calved in a typical 4 yr interval, suggesting no apparent immediate impact of tagging on reproduction for these individuals, but longer-term monitoring is needed. There was no observable difference in the body condition between the 2 tagged and non-tagged females. Ongoing monitoring post-tagging is required to build on the sample size and statistical power. We demonstrate the value of long-term monitoring programmes and a collaborative approach for evaluating effects from satellite-tagging cetaceans to support species management.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"155 ","pages":"125-140"},"PeriodicalIF":1.4,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Diseases of aquatic organisms
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1