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Preliminary study on otolith chemistry and otolith morphology of two demersal fish species, European hake Merluccius merluccius (Linnaeus, 1758) and striped red mullet (Mullus surmuletus, Linnaeus, 1758) in the Sea of Marmara 马尔马拉海两种底生鱼类Merluccius Merluccius (Linnaeus, 1758)和条纹红鲻鱼(Mullus surmuletus, Linnaeus, 1758)耳石化学和耳石形态的初步研究
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.13
H. Bal, S. Esen
Otoliths are calcium carbonate (CaCO3) accumulations. Under the influence of different ecosystems morphological and chemical composition change. In this study, economically important two demersal fish species; European hake Merluccius merluccius (Linnaeus, 1758) and Stripet red mullet (Mullus surmuletus Linnaeus, 1758) was examined. Otoliths (Sagitta) belonging to these two species have been studied both chemically and morphologicall. Morphometric measurements of otoliths (length, mm; width, mm; area, mm2; perimeter, mm) in the sagittal of each species was made by the Leica M125 tri-ocular microscope. In the chemical analysis of otoliths, strontium (Sr), magnesium (Mg) and calcium (Ca) trace element amounts, the ratios of Sr and Mg trace elements to Ca element (Sr/Ca and Mg/Ca) were determined. For the micro-chemical analysis of otoliths ICP-MS was used. The highest magnesium (24.92±9.57 mmol/mol) and strontium (26.17±1.81 mmol/mol) element values were found in the otolith of red mullet. The difference between strontium (Sr) and magnesium (Mg) amounts for two fish species was found to be significant (P<0.05). In addition to it was found that the difference between them in the amount of calcium is significant (P<0.001). The shape indexes of otoliths are significantly different between the two fish species. Especially in terms of roundness (RD) and aspect ratio (AR) (P<0.001). The results of this study provide information about the habitats of two economic importance demersal fish species. Since such studies can give information about the habitat areas of fish species, they are important for tracking stocks, migration routes and sustainable fisheries.
耳石是碳酸钙(CaCO3)聚集物。在不同生态系统的影响下,土壤形态和化学成分发生了变化。在本研究中,经济上重要的两种底栖鱼类;对欧洲鲱鱼Merluccius Merluccius (Linnaeus, 1758)和赤鲻(Mullus surmuletus Linnaeus, 1758)进行了研究。属于这两个物种的耳石(矢状岩)已经进行了化学和形态学的研究。耳石的形态测量(长度,mm;毫米宽;区域,平方毫米;各物种矢状面周长(mm)用徕卡M125三眼显微镜观察。在耳石化学分析中,测定了微量元素锶(Sr)、镁(Mg)和钙(Ca)的含量,测定了微量元素锶、镁与钙的比值(Sr/Ca和Mg/Ca)。采用ICP-MS对耳石进行微化学分析。红鲻鱼耳石中镁元素(24.92±9.57 mmol/mol)和锶元素(26.17±1.81 mmol/mol)含量最高。两种鱼类的锶(Sr)和镁(Mg)含量差异显著(P<0.05)。此外,发现两者之间钙含量差异显著(P<0.001)。两种鱼类的耳石形状指标存在显著差异。特别是在圆度(RD)和纵横比(AR)方面(P<0.001)。本研究结果提供了两种经济上重要的底栖鱼类的栖息地信息。由于这种研究可以提供有关鱼类生境的资料,因此对追踪鱼类种群、迁徙路线和可持续渔业很重要。
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引用次数: 0
Monthly variation of micro- and macro-element composition in smooth scallop, Flexopecten glaber (Linnaeus, 1758), from the Çardak Lagoon (Çanakkale Strait, Turkey) Çardak泻湖(Çanakkale海峡)Flexopecten glaber (Linnaeus, 1758)光滑扇贝微量元素和常量元素组成的月变化
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.06
P. Vural, S. Acarlı
The present study investigated the total elemental composition (Fe, Cu, Mn, Zn, Al, S, B, Na, Mg, K, Ca, P, S) in smooth scallop (Flexopecten glaber) meat collected monthly in the Çardak Lagoon (Turkey) in 2017- 2018. Considering the distribution of elements in terms of quantity during the year, the elements were sorted as S> Na> K> P> Mg> Ca> Fe> Zn> Mn> Al> B> Cu> Se and the heavy metals were below the detection limit. Although the elements were at their highest values during autumn and summer, they were at their lowest values during spring and winter. The differences between the months in terms of Fe, Mn, Mg, and Ca elements were found to be statistically significant (p <0.05). It was determined that the element composition of smooth scallop meat was suitable for consumption in terms of the area where it is collected and is beneficial for health when consumed in the amounts recommended by FAO/WHO, and CODEX. The Hazard Quotient (THQ) and the Hazard Index (HI), which was conducted to evaluate the health risks of consuming smooth
本研究调查了2017- 2018年在Çardak泻湖(土耳其)每月采集的光滑扇贝(Flexopecten glaber)肉中的总元素组成(Fe, Cu, Mn, Zn, Al, S, B, Na, Mg, K, Ca, P, S)。从年内元素数量分布来看,元素排序为S> Na> K> P> Mg> Ca> Fe> Zn> Mn> Al> B> Cu> Se,重金属均低于检出限。元素含量在秋季和夏季最高,而在春季和冬季最低。月份间Fe、Mn、Mg、Ca元素含量差异有统计学意义(p <0.05)。经确定,顺滑扇贝肉的元素组成适合于采集扇贝肉的地区食用,如果按照粮农组织/世卫组织和食品法典委员会建议的量食用,则对健康有益。采用危害商数(THQ)和危害指数(HI)对食用香椿的健康风险进行了评价
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引用次数: 4
The effect of mercury, copper, and zinc on paraoxonase (PON) enzyme activity in Bonito (Sarda sarda) fish 汞、铜和锌对鲣鱼对氧磷酶(PON)活性的影响
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.09
Busra Sahin, S. Çenesiz
Since heavy metal dirtiness, which we frequently encounter in environmental pollution causes harmful effects on the organism through biochemical enzyme reactions, in this study, the effects of mercury (Hg+2), copper (Cu+2), and zinc (Zn+2) heavy metal ions, which are common in environmental pollution, on PON (paraoxonase) enzyme activity in muscle tissue of bonito (Sarda sarda) were investigated. In the study, 25 bonito (S. sarda) fish muscle tissues freshly obtained from the Samsun region sea were used. The changes in PON enzyme activity were determined by adding different volumes of heavy metal solutions. PON enzyme activities of Hg+2 heavy metal ion used in different volumes were calculated as 30.9383 U/mLdak, 29.0598 U/mLdak, 26.3799 U/mLdak, 23.9443 U/mLdak, 20.6725 U/mLdak, PON enzyme activities of Cu+2 heavy metal ion used in different volumes were calculated as 19.7949 U/mLdak, 19.4807 U/mLdak 19.1864 U/mLdak, 19.1200 U/mLdak, 18.9037 U/mLdak and PON enzyme activities of Zn+2 heavy metal ion used in different volumes were calculated as 23.8305 U/mLdak, 23.0781 U/mLdak, 22.9073 U/mLdak, 22.4324 U/mLdak, 21.8159 U/mLdak. As a result of these obtained data, activity (%) values were calculated and activity (%) graphs were drawn. As a result of the study, it was determined that increasing concentrations of Cu+2 and Zn+2 heavy metal ions caused a decrease in PON enzyme activity, but there was no statistically significant difference between the different concentrations used. It was determined that increasing concentrations of Hg+2 heavy metal ion inhibited the PON enzyme activity, caused a statistically significant decrease between the activities depending on the different concentrations used (p < 0.05).
由于我们在环境污染中经常遇到的重金属污垢通过生化酶反应对生物体产生有害影响,本研究研究了环境污染中常见的汞(Hg+2)、铜(Cu+2)和锌(Zn+2)重金属离子对鲣鱼肌肉组织PON(对氧氧化酶)酶活性的影响。在研究中,25鲣鱼(S. sarda)的肌肉组织新鲜获得的三顺地区的海。通过添加不同体积的重金属溶液来测定PON酶活性的变化。不同体积下Hg+2重金属离子的PON酶活性分别为30.9383 U/mLdak、29.0598 U/mLdak、26.3799 U/mLdak、23.9443 U/mLdak、20.6725 U/mLdak,不同体积下Cu+2重金属离子的PON酶活性分别为19.7949 U/mLdak、19.4807 U/mLdak、19.1864 U/mLdak、19.1200 U/mLdak、18.9037 U/mLdak,不同体积下Zn+2重金属离子的PON酶活性分别为23.8305 U/mLdak、23.0781 U/mLdak、22.9073 U/mLdak,22.4324 U/mLdak, 21.8159 U/mLdak。根据这些获得的数据,计算活性(%)值并绘制活性(%)图。研究结果确定,Cu+2和Zn+2重金属离子浓度的增加会导致PON酶活性的降低,但不同浓度之间的差异无统计学意义。结果表明,Hg+2重金属离子浓度的升高对PON酶活性有抑制作用,不同浓度对PON酶活性的影响有统计学意义(p < 0.05)。
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引用次数: 0
Diversity of benthic macroinvertebrates and water quality of Karasu Stream (Black Sea) 黑海卡拉苏河底栖大型无脊椎动物多样性与水质
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.08
Eylem Aydemir Çil, Murat Özbek, Öztekin Yardım, S. Yıldız, Ayşe Taşdemir, Hamidreza Rasouli, Pınar Gürbüzer
The study was conducted in Karasu Stream (Sinop Province, Black Sea Region of Turkey). The purpose of this study is, to determine the benthic macroinvertebrate composition of the stream, together with some of its environmental characteristics (water temperature, pH and dissolved oxygen) to evaluate the trophic level of the stream. Samplings of benthic macroinvertebrates and environmental variables were performed monthly at ten stations between February 2013 and January 2014. As a result, 18260 specimens were investigated and 175 taxa were determined. Chironomidae and Oligochaeta were the higher groups in terms of species richness with 48 and 38 taxa, respectively. The BMWP and ASPT indices indicate that all the stations belong to “slightly polluted (Class II)” or “unpolluted (Class I)” water quality levels.
该研究在卡拉苏溪(土耳其黑海地区Sinop省)进行。本研究的目的是确定该河流的底栖大型无脊椎动物组成,并结合其一些环境特征(水温、pH值和溶解氧)来评估该河流的营养水平。2013年2月至2014年1月,在10个站点每月进行底栖大型无脊椎动物和环境变量采样。共调查标本18260份,鉴定类群175个。Chironomidae和Oligochaeta是物种丰富度最高的类群,分别有48和38个类群。BMWP和ASPT水质指数均为“轻度污染(II类)”或“未污染(I类)”水质水平。
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引用次数: 5
An analysis of historical landing data of some pelagic species caught by purse seines in the Aegean Sea 爱琴海被围网捕获的一些远洋物种的历史登陆数据分析
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.03
Z. Tosunoğlu, Tevfik Ceyhan
The fishing activity with purse seines in the Aegean Sea accounts for almost half of the pelagic fish excluding anchovy (Engraulis encrasicolus Linnaeus, 1758) in Turkey. In this study, we aimed to analyse the catch amounts of some important pelagic species caught by Aegean purse seiners between 1973 and 2019. While mid-1990s are the most captured years for the European pilchard (Sardina pilchardus Walbaum, 1792) in Aegean Sea and also Turkish Seas, this amount has decreased at the level of 10000 mt in the mid-2000s and remained similar amounts up to now. Though European pilchard was the most caught species in the Aegean until 2011, this has changed in favour of anchovy in the last decade. Recently, almost all pelagic species such as mackerel (Scomber scombrus Linnaeus, 1758), chub mackerel (Scomber japonicus Houttuyn, 1782) and bogue (Boops boops Linnaeus, 1758) have been caught from the Aegean Sea in Turkey. In the study, the production of these species in the historical period was tried to be associated with the regulations implemented with circulars and notifications, and the problems preventing the sustainable use of these species that were caught by purse seiners were determined and solutions were proposed for the future.
在土耳其,除凤尾鱼(engrulis encrasicolus Linnaeus, 1758)外,爱琴海的围网捕鱼活动几乎占了中上层鱼类的一半。在这项研究中,我们旨在分析1973年至2019年爱琴海围网捕获的一些重要远洋物种的捕获量。虽然1990年代中期是爱琴海和土耳其海欧洲沙丁鱼捕获最多的年份(Sardina pilchardus Walbaum, 1792),但这一数量在2000年代中期下降到10000吨的水平,到现在仍然保持类似的数量。尽管在2011年之前,欧洲沙丁鱼是爱琴海捕捞最多的物种,但在过去的十年里,凤尾鱼的捕捞量有所改变。近年来,在土耳其爱琴海捕获了鲭鱼(Scomber scombrus Linnaeus, 1758)、鲐鱼(Scomber japonicus Houttuyn, 1782)和鲭鱼(Boops Boops Linnaeus, 1758)等几乎所有的中游鱼类。在研究中,试图将这些物种在历史时期的产量与通过通告和通知实施的法规联系起来,并确定了阻碍围网捕捞这些物种可持续利用的问题,并提出了未来的解决方案。
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引用次数: 0
Evaluation of water quality of Çoruh River Basin (Turkey) using some biotic indices 利用生物指标评价土耳其Çoruh河流域水质
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.01
Hazel Baytaşoğlu, A. M. Gözler
In this study, it was aimed to evaluate the water quality of the Çoruh Basin using BMWP (Biological Monitoring Working Party) index, ASPT (Average Score Per Taxon) index, Shannon-Wiener Diversity Index, EPT (Ephemeroptera Plecoptera Trichoptera taxa), EP (Ephemeroptera Plecoptera taxa) index scores and benthic macroinvertebrates. The benthic macroinvertebrates were collected from 54 stations at the Çoruh basin between 2014-2016 years. Standard hand net (D-frame net) and Ekman-Birge grab were used as sampling tools. As a result of the diagnoses, a total of 7246 individuals belonging to Insecta, Crustacea, Mollusca, Oligochaeta and Plathyhelminthes were obtained. It was determined that the most dominant group was Insecta and the rarest group was Plathyhelminthes. It was observed that the BMWP score ranged between 5 and 94, and the lowest and highest number of families detected in the stations were 1 and 18, respectively. It was determined that the Shannon Wiener diversity index value was between 0.54-2.20, therefore the basin streams generally showed moderate pollution. The results of BMWP index show that the basin streams had mostly show 3rd and 4th class water quality and also biodiversity decreases with the deterioration of the riverbed or exposure to pollution.
采用BMWP (Biological Monitoring Working Party)指数、ASPT (Average Score Per Taxon)指数、Shannon-Wiener多样性指数、EPT (Ephemeroptera Plecoptera Trichoptera taxa)、EP (Ephemeroptera Plecoptera taxa)指数评分和底栖大型无脊椎动物对Çoruh流域的水质进行了评价。2014-2016年,在Çoruh流域的54个站点采集了大型底栖无脊椎动物。采用标准手网(d -框架网)和Ekman-Birge抓斗作为采样工具。共获得昆虫科、甲壳类、软体类、寡毛类和贝壳类共7246只。结果表明,昆虫纲为优势类群,最稀有类群为Plathyhelminthes。BMWP分值在5 ~ 94分之间,检测到的最低户数为1户,最高户数为18户。Shannon Wiener多样性指数在0.54 ~ 2.20之间,表明流域河流总体呈中度污染。BMWP指数结果表明,流域河流水质多为3、4级,生物多样性随河床恶化或污染程度的增加而减少。
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引用次数: 2
Size and structure of the Mediterranean medicinal leech, Hirudo verbana populations inhabiting wetlands around Lake Eğirdir, Turkey 地中海药用水蛭的大小和结构,居住在土耳其Eğirdir湖附近湿地的水蛭种群
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.05
M. Ceylan, O. Çetinkaya
In the present study size and structure of the Mediterranean medicinal leech (Hirudo verbana Carena, 1820) populations inhabiting wetlands around Lake Eğirdir (Turkey) were investigated. Population size was estimated by removal methods, age classes were estimated for the first time in leeches (Hirudinea) using “Modal Progression Analysis” of body length frequencies using Bhattacharya's method. The population size and biomass of medicinal leeches around Lake Eğirdir were estimated to be 1,562,696 ± 805,613 leeches and 467.26 ± 172.91 kg, respectively. Three age classes were identified; the age group of 0+ was dominant with 78.6%. While 89.9% of the individuals weighed less than 1 g, whereas the broodstock was estimated to represent only 7%. Body weight, body length and condition factor were found to be 0.52 ± 1.19 g, 4.5 ± 2.4 cm and 0.620 ± 0.123, respectively. This study reveals that the population size of H. verbana in the wetlands around Lake Eğirdir is relatively well preserved and there is no significant threat to the sustainability of the exploitation of the medicinal leech populations. It is concluded that the stability of the lake's water budget, the variety and abundance of the host in the undamaged wetlands, the socioeconomic structure of the leech collectors, the sales policies, and the effective activities of conservation and control units might affect conservation status of the medicinal leech populations inhabiting wetlands around Lake Eğirdir.
本研究调查了居住在土耳其Eğirdir湖附近湿地的地中海药用水蛭(Hirudo verbana Carena, 1820)种群的大小和结构。采用去除法对水蛭种群规模进行估计,采用Bhattacharya法对水蛭体长频率进行“模态递进分析”,首次对水蛭的年龄等级进行估计。Eğirdir湖周边药用水蛭种群数量为1,562,696±805,613只,生物量为467.26±172.91 kg。确定了三个年龄组;0+年龄组占主导地位,占78.6%。而89.9%的个体体重不足1克,而据估计,亲鱼只占7%。体重为0.52±1.19 g,体长为4.5±2.4 cm,体质因子为0.620±0.123。研究结果表明,Eğirdir湖周边湿地水蛭种群规模保存较好,对药用水蛭种群的可持续性开发利用没有明显威胁。结果表明:湖泊水量平衡的稳定性、未受损湿地寄主的多样性和丰度、水蛭收集者的社会经济结构、销售政策以及保护和控制单位的有效活动可能会影响环湖湿地药用水蛭种群的保护状况Eğirdir。
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引用次数: 3
About the identity of Granulina pusaterii Smriglio & Mariottini, 2003 (Volutoidea: Granulinidae) from the Tunisian Plateau 关于来自突尼斯高原的Granulina pusaterii Smriglio & Mariottini, 2003 (Volutoidea: Granulinidae)的鉴定
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.07
F. Boyer, Walter Renda, Attilio Pagli
On the ground of an extensive study of topotypes and of the shell morphology documented in comparable Mediterranean populations, Granulina pusaterii Smriglio & Mariottini, 2003 described from the Tunisian Plateau is proved to belong to the natural variability of G. melitensis Smriglio, Mariottini & Rufini, 1998 and it is proposed as junior synonym name of the latter.
在对地中海同类种群的形态和壳形态进行广泛研究的基础上,来自突尼斯高原的Granulina pusaterii Smriglio & Mariottini, 2003被证明属于G. melitensis Smriglio, Mariottini & Rufini, 1998的自然变异,并被建议作为后者的初级同义词。
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引用次数: 0
Characterization of Planococcus dechangensis isolated from a water sample of Çamaltı Saltern 从Çamaltı Saltern水样中分离的去变Planococcus的特性
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.15
P. Çağlayan
In the present study, strain MHDS3 was isolated from a water sample of Çamaltı Saltern and identified using conventional and molecular methods. 16S rRNA gene sequence analyses showed that the strain MHDS3 belonged to Planococcus dechangensis species. It gave a positive result in the Gram staining test. The cells were coccus, non-motile, aerobic, catalase positive, oxidase negative and the colony pigmentation was yellow-orange. It showed negative results for citrate utilization, indole production from tryptophane, Voges-Proskauer and methyl red. This isolate was able to grow at 10-45°C (optimally 35°C), pH 6-8 (optimally pH 7) and 3-20% NaCl (optimally 10% NaCl). It was not able to grow at 4°C, 10°C, 50°C, salt-free, 0.5%, 25%, %30 total salt, pH 4-5, and pH 9-12. Glucose, ribose, fructose, sucrose, maltose were used by the test isolate as carbon sources. Different amino acids found in the structure of animal hide such as L-lysine, L-arginine, L-cysteine, L-alanine, L-tyrosine, L-histidine were also utilized by the bacterium. During the salt production process, this bacterium may contaminate the salt which is used in the food and leather industries. The activities of harmful moderately halophilic bacteria should be prevented by effective antimicrobial applications.
在本研究中,从Çamaltı Saltern的水样中分离到菌株MHDS3,并使用常规和分子方法对其进行了鉴定。16S rRNA基因序列分析表明,菌株MHDS3属于脱变Planococcus dechangensis。革兰氏染色试验呈阳性。细胞呈球菌状,无运动,需氧,过氧化氢酶阳性,氧化酶阴性,菌落色素为黄橙色。在柠檬酸利用率、色氨酸吲哚产量、Voges-Proskauer和甲基红产量方面均呈阴性。该菌株能在10-45°C(最佳35°C)、pH 6-8(最佳pH 7)和3-20% NaCl(最佳10% NaCl)条件下生长。在4°C、10°C、50°C、无盐、0.5%、25%、%30总盐、pH 4-5和pH 9-12条件下均不能生长。实验分离物以葡萄糖、核糖、果糖、蔗糖、麦芽糖为碳源。在动物皮结构中发现的不同氨基酸,如l -赖氨酸、l -精氨酸、l -半胱氨酸、l -丙氨酸、l -酪氨酸、l -组氨酸也被细菌利用。在盐的生产过程中,这种细菌可能会污染用于食品和皮革工业的盐。有害的中度嗜盐细菌的活动应通过有效的抗菌应用加以阻止。
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引用次数: 0
Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets 以甾体激素、天然激素和木瓜籽为基础的饲料饲喂尼罗提鱼(Oreochromis niloticus)的鳃、肝脏和肌肉组织学变化
Pub Date : 2021-12-15 DOI: 10.12714/egejfas.38.4.10
E. Orose, O. K. Wokeh, E. Odioko
This study was conducted to evaluate the histological changes of 17αmethyl-testosterone (MT), wet testes of bull (Bos indicus), boar (Sus domesticus), bull/mud catfish testes (B. indicus/C. gariepinus) and pawpaw (Carica papaya) seed powder-based diet on the Nile tilapia fry in an indoor experimental pond. Histological changes and efficacies in producing monosex males were determined after four months. Fifty-five fry were assigned randomly to three experimental ponds in triplicates. Five experimental feeds composed of soya-bean, fish meal, wheat bran, cassava flour were formulated by adding 0.06g of 17α- methyl-testosterone, 17.47g of bull wet testes, 25.59g of boar wet testes, 20.48g of bull/catfish and 20g of pawpaw seed powder per kg of feed to group 1, 2, 3, 4, 5 respectively. Obtained results from the morphological examination revealed a significant difference (p < 0.05) in phenotypic males of the Nile tilapia fry fed with the experimental diets. The highest indistinct percentage was recorded in the 17αmethyl-testosterone-treated diet while the lowest was recorded in diets 3 and 4 with no significant differences. All the groups showed histopathological differences in the livers, gills, and muscles. However, the liver histology of groups 1 and 5 showed central vessels that are mildly congested with abundant glycogen vacuolation and somewhat fat infiltration, while the histology of the muscles of group 5 showed pathological lesions including moderate to severe necrosis. These alterations can be considered an indication of the performance and health of fish in monosex culture medium indicating effects of plant extract and MT.
本研究对17α甲基睾酮(MT)、牛(Bos indicus)、野猪(Sus domesticus)、牛/泥鲶(B. indicus/C. catfish)湿睾丸的组织学变化进行了研究。在室内实验池中对尼罗罗非鱼鱼苗进行了以种子粉为基础的饲料研究。4个月后测定组织学变化和产生单性雄性的效果。将55尾鱼苗随机分配到3个试验池中,每组3次。以大豆、鱼粉、麦麸、木薯粉为原料,每kg饲料分别添加0.06g 17α-甲基睾酮、17.47g公牛湿睾丸、25.59g公猪湿睾丸、20.48g公牛/鲶鱼和20g木瓜籽粉,配制成1、2、3、4、5组。形态检测结果显示,不同饲料对尼罗罗非鱼鱼苗表型差异显著(p < 0.05)。17α甲基睾酮组的模糊率最高,而3、4组的模糊率最低,差异不显著。各组在肝脏、鳃和肌肉方面均表现出组织病理学差异。但1组和5组肝脏组织学表现为中心血管轻度充血,糖原空泡丰富,有脂肪浸润;5组肌肉组织学表现为中重度坏死等病理病变。这些变化可以被认为是单性培养基中鱼类生产性能和健康状况的指示,表明植物提取物和MT的作用。
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引用次数: 0
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Ege Journal of Fisheries and Aquatic Sciences
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