Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.12
Tuğçe Mutaf, G. Çalışkan, S. Öncel, M. Elibol
Green synthesis of metal nanoparticles through biological resources has attracted attention in recent years. The main reason for that, a lot of toxic chemicals as reducing and stabilizing agents are used in physical and chemical methods which are known as conventional methods. Organisms such as plants, fungi, bacteria, and algae are alternative sources for green nanoparticle synthesis because of their more eco-friendly nature and not be a threat to human health. Microalgae as aquatic microorganisms have been added into the formulations of food, cosmetics, and pharmaceutical for many years, due to their high value-added metabolites such as proteins, vitamins, pigments, fatty acids, intracellular and extracellular polysaccharides. In addition, microalgae have a high potential in biogenic nanoparticle synthesis because of their metal ions accumulation capability, phytoremediation potential, and rich in intracellular and extracellular metabolites that will reduce metal ions to elemental state. In recent years, the number of studies, focused on silver, gold, titanium, zinc, iron, etc. nanoparticle synthesis from many microalgae species by intracellular and extracellular pathways has increased. This review article aims to provide a brief outline of microalgae and cyanobacteria species studied in the context of nanoparticle synthesis, different approaches for nanoparticle synthesis from microalgae, the mechanism of nanoparticle synthesis, and basic characterization principles and antimicrobial activities of nanoparticles produced by green synthesis.
{"title":"Green synthesis of metal nanoparticles by microalgae","authors":"Tuğçe Mutaf, G. Çalışkan, S. Öncel, M. Elibol","doi":"10.12714/egejfas.40.1.12","DOIUrl":"https://doi.org/10.12714/egejfas.40.1.12","url":null,"abstract":"Green synthesis of metal nanoparticles through biological resources has attracted attention in recent years. The main reason for that, a lot of toxic chemicals as reducing and stabilizing agents are used in physical and chemical methods which are known as conventional methods. Organisms such as plants, fungi, bacteria, and algae are alternative sources for green nanoparticle synthesis because of their more eco-friendly nature and not be a threat to human health. Microalgae as aquatic microorganisms have been added into the formulations of food, cosmetics, and pharmaceutical for many years, due to their high value-added metabolites such as proteins, vitamins, pigments, fatty acids, intracellular and extracellular polysaccharides. In addition, microalgae have a high potential in biogenic nanoparticle synthesis because of their metal ions accumulation capability, phytoremediation potential, and rich in intracellular and extracellular metabolites that will reduce metal ions to elemental state. In recent years, the number of studies, focused on silver, gold, titanium, zinc, iron, etc. nanoparticle synthesis from many microalgae species by intracellular and extracellular pathways has increased. This review article aims to provide a brief outline of microalgae and cyanobacteria species studied in the context of nanoparticle synthesis, different approaches for nanoparticle synthesis from microalgae, the mechanism of nanoparticle synthesis, and basic characterization principles and antimicrobial activities of nanoparticles produced by green synthesis.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82710465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.05
Tuba Elhazar, B. Kaya, F. Caf
In this study, protein extraction was performed from the aquatic insect Helophorus aquaticus (Linnaeus, 1758) and Helophorus syriacus (Kuwert, 1885) species belonging to the genus Helophorus (Coleoptera: Helophoridae). Then, these protein extracts were studied in vitro. These species were collected from the shallow parts of various streams, springs, creeks, ponds and hot springs from Bingöl city centre and its districts between May to June 2017. The protein amount was determined 34.78 mg/mL in the H. aquaticus and 35.14 mg/mL in the H. syriacus, after that he antioxidant capacity of protein extracts was examined. Metal chelating activity was determined as 90-88.5% and DPPH removal activity 53.19-61.7% for H. aquaticus and H. syriacus respectively. Protein samples belonging to both species were tested for cell vitality with WST-1 in PC-3 (prostate cancer) cells with in vitro cell culture. Upon examination of the test results, it has been found out that protein extracts from both of the studied species caused a decrease in cell inhibition. The highest cell inhibition was observed in samples with 1000 μg/mL insect protein extract added. In this study, protein expression providing apoptosis was examined with the Western blot technique after the effective dose was established. By looking at the proteins of Cyt-C and Caspase 3 with the Western blot technique, the efficacy of the protein extracts from both species was demonstrated effectively for the in vitro PC-3 line in non-apoptosis cell death. As a result of the study, insect proteins were shown to support the production of proteins that ensure cell death with the western blot technique.
{"title":"Determination of anti-cancer and antioxidant properties of protein extracts obtained from aquatic Helophorus (Coleoptera: Helophoridae) insects","authors":"Tuba Elhazar, B. Kaya, F. Caf","doi":"10.12714/egejfas.40.1.05","DOIUrl":"https://doi.org/10.12714/egejfas.40.1.05","url":null,"abstract":"In this study, protein extraction was performed from the aquatic insect Helophorus aquaticus (Linnaeus, 1758) and Helophorus syriacus (Kuwert, 1885) species belonging to the genus Helophorus (Coleoptera: Helophoridae). Then, these protein extracts were studied in vitro. These species were collected from the shallow parts of various streams, springs, creeks, ponds and hot springs from Bingöl city centre and its districts between May to June 2017. The protein amount was determined 34.78 mg/mL in the H. aquaticus and 35.14 mg/mL in the H. syriacus, after that he antioxidant capacity of protein extracts was examined. Metal chelating activity was determined as 90-88.5% and DPPH removal activity 53.19-61.7% for H. aquaticus and H. syriacus respectively. Protein samples belonging to both species were tested for cell vitality with WST-1 in PC-3 (prostate cancer) cells with in vitro cell culture. Upon examination of the test results, it has been found out that protein extracts from both of the studied species caused a decrease in cell inhibition. The highest cell inhibition was observed in samples with 1000 μg/mL insect protein extract added. In this study, protein expression providing apoptosis was examined with the Western blot technique after the effective dose was established. By looking at the proteins of Cyt-C and Caspase 3 with the Western blot technique, the efficacy of the protein extracts from both species was demonstrated effectively for the in vitro PC-3 line in non-apoptosis cell death. As a result of the study, insect proteins were shown to support the production of proteins that ensure cell death with the western blot technique.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90153857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.09
Büşra Yamaç, T. Şişman
In this study, the karyotypic characteristics of Chondrostoma regium (Heckel, 1843) have been investigated. Fish samples were caught from the Karasu River (Euphrates River Basin) with fishing net. The live fish were transported to the laboratory and kept in aerated aquaria before the analyses. The karyotype analysis was performed in fish kidney and gill epithelium cells. It was determined that C. regium had 2n=50 chromosomes. In detail, the karyotype formula of C. regium was determined as 9 metacentric, 7 submetacentric, 1 subtelocentric and 8 telocentric chromosome pairs (18M+14SM+2ST+16T), and fundamental arm number was calculated as 82. Constitutive heterochromatin regions were determined on telomeres of the chromosomes. Nuclear orgnizer regions were detected on 21st chromosome. Karyotype symmetry/asymmetry index was calculated as 2.32. The karyotypes of gill and kidney cells were the same. No sex chromosomes were cytologically detected.
{"title":"Karyotypic analysis of Chondrostoma regium (Teleostei: Leuciscidae) distributed in the Karasu River (Erzurum)","authors":"Büşra Yamaç, T. Şişman","doi":"10.12714/egejfas.40.1.09","DOIUrl":"https://doi.org/10.12714/egejfas.40.1.09","url":null,"abstract":"In this study, the karyotypic characteristics of Chondrostoma regium (Heckel, 1843) have been investigated. Fish samples were caught from the Karasu River (Euphrates River Basin) with fishing net. The live fish were transported to the laboratory and kept in aerated aquaria before the analyses. The karyotype analysis was performed in fish kidney and gill epithelium cells. It was determined that C. regium had 2n=50 chromosomes. In detail, the karyotype formula of C. regium was determined as 9 metacentric, 7 submetacentric, 1 subtelocentric and 8 telocentric chromosome pairs (18M+14SM+2ST+16T), and fundamental arm number was calculated as 82. Constitutive heterochromatin regions were determined on telomeres of the chromosomes. Nuclear orgnizer regions were detected on 21st chromosome. Karyotype symmetry/asymmetry index was calculated as 2.32. The karyotypes of gill and kidney cells were the same. No sex chromosomes were cytologically detected.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78324320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.11
B. Öztürk, Banu Bitlis, N. Türkçü
The present study is dealing with three Circulus species recorded along the Turkish coasts (İskenderun Bay, Levantine coast of Türkiye). Of the identified species, Circulus novemcarinatus and Circulus octoliratus are non-indigenous species originated outside the Mediterranean Sea, whereas Circulus striatus is a native one distributed in the eastern Atlantic Ocean and Mediterranean Sea. While Circulus novemcarinatus was recorded at depths between 9 and 60 m, Circulus octoliratus and Circulus striatus were encountered at shallow depths (5-11.7 m, respectively). Within the present study some morphological and distributional characteristics of the investigated species are described.
{"title":"Circulus (Mollusca-Gastropoda) species of the Turkish coasts with a note on the presence of Circulus octoliratus (Carpenter, 1856)","authors":"B. Öztürk, Banu Bitlis, N. Türkçü","doi":"10.12714/egejfas.40.1.11","DOIUrl":"https://doi.org/10.12714/egejfas.40.1.11","url":null,"abstract":"The present study is dealing with three Circulus species recorded along the Turkish coasts (İskenderun Bay, Levantine coast of Türkiye). Of the identified species, Circulus novemcarinatus and Circulus octoliratus are non-indigenous species originated outside the Mediterranean Sea, whereas Circulus striatus is a native one distributed in the eastern Atlantic Ocean and Mediterranean Sea. While Circulus novemcarinatus was recorded at depths between 9 and 60 m, Circulus octoliratus and Circulus striatus were encountered at shallow depths (5-11.7 m, respectively). Within the present study some morphological and distributional characteristics of the investigated species are described.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80093296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.03
Münevver Oral
Next generation sequencing (NGS) technologies constitute the most powerful scientific advance of 21st century with a promise of fast and cost effective data generation in biology. Yet, up to date NGS studies remain often limited to laboratories with established resources. In the present study, we employed construction of ddRADseq library by using routine lab consumables (agarose gel electrophoresis: AGE thereafter) compared to high-tech NGS consumables (paramagnetic beads) during size selection. The ddRADseq library was constructed for sequencing size selected based on universally used paramagnetic beads, while remaining aliquot was used as a template to assess the feasibility of ddRADseq library construction using AGE for labs with limited resources. Both libraries were optimised for 15 PCR cycles indicating similarity in template intensity. Post-PCR quantification of the libraries was comparable (~10 ng.µL-1). Size distribution assessment revealed a cleaner pick at the ddRADseq library size selected manually based on AGE. Similarly, intercalating agent of Qubit confirmed the quantity of libraries was similar (>3 ng.µL-1). Although being more time consuming due to pre-electrophoresis preparations, serial wash and staining steps, ddRADseq library construction is achievable using routine lab consumables provided to supply the adaptors and PCR primers for the initial wet-lab work. These results manifest the feasibility of ddRADseq library generation for labs with limited resources.
{"title":"A cost effective alternative method to ddRADseq library construction during size selection","authors":"Münevver Oral","doi":"10.12714/egejfas.40.1.03","DOIUrl":"https://doi.org/10.12714/egejfas.40.1.03","url":null,"abstract":"Next generation sequencing (NGS) technologies constitute the most powerful scientific advance of 21st century with a promise of fast and cost effective data generation in biology. Yet, up to date NGS studies remain often limited to laboratories with established resources. In the present study, we employed construction of ddRADseq library by using routine lab consumables (agarose gel electrophoresis: AGE thereafter) compared to high-tech NGS consumables (paramagnetic beads) during size selection. The ddRADseq library was constructed for sequencing size selected based on universally used paramagnetic beads, while remaining aliquot was used as a template to assess the feasibility of ddRADseq library construction using AGE for labs with limited resources. Both libraries were optimised for 15 PCR cycles indicating similarity in template intensity. Post-PCR quantification of the libraries was comparable (~10 ng.µL-1). Size distribution assessment revealed a cleaner pick at the ddRADseq library size selected manually based on AGE. Similarly, intercalating agent of Qubit confirmed the quantity of libraries was similar (>3 ng.µL-1). Although being more time consuming due to pre-electrophoresis preparations, serial wash and staining steps, ddRADseq library construction is achievable using routine lab consumables provided to supply the adaptors and PCR primers for the initial wet-lab work. These results manifest the feasibility of ddRADseq library generation for labs with limited resources.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85850321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.04
Gökhan Tunçelli, İdil Can Tunçelli, Devrim Memiş
We determined the number of coliform bacteria, Escherichia coli, yeast, and molds that may occur in the system, and the quality of lettuce grown in aquaponics for consumers from sensory, colour, and texture points of view. The amount of yeast and mold in the plant growing medium (hydroton) and water was 4.67 log CFU/cm2 and 2.25 log CFU/mL at the end of the six-week experiment, respectively. The number of coliform bacteria and E. coli in the growing medium and in the system water was found to be 2.57 log CFU/cm2 and 3.46 log CFU/mL for coliform, 0.75 log CFU/cm2 0.31 log CFU/mL for E. coli, respectively. Organisms that pose a risk to food safety, accumulate in the culture media. After the harvest, lettuce cultured in the aquaponic system (AP) was compared with the lettuce cultured in soil (SC). According to the results, AP lettuce was found to have darker colors (Lightness: 56.4 AP, 49.09 SC, p<0.05), harder (Hardness: 209.3 AP, 153.7 SC, p<0.05), and slightly appetizing (Sensory analysis overall liking: 8.4 AP, 7.7 SC) than SC. In conclution, aquaponic systems are much more preferable in terms of sensory quality and consumer preferences than soil-based production systems.
{"title":"Evaluation of lettuce (Lactuca sativa L.) in aquaponic system in terms of food safety","authors":"Gökhan Tunçelli, İdil Can Tunçelli, Devrim Memiş","doi":"10.12714/egejfas.40.1.04","DOIUrl":"https://doi.org/10.12714/egejfas.40.1.04","url":null,"abstract":"We determined the number of coliform bacteria, Escherichia coli, yeast, and molds that may occur in the system, and the quality of lettuce grown in aquaponics for consumers from sensory, colour, and texture points of view. The amount of yeast and mold in the plant growing medium (hydroton) and water was 4.67 log CFU/cm2 and 2.25 log CFU/mL at the end of the six-week experiment, respectively. The number of coliform bacteria and E. coli in the growing medium and in the system water was found to be 2.57 log CFU/cm2 and 3.46 log CFU/mL for coliform, 0.75 log CFU/cm2 0.31 log CFU/mL for E. coli, respectively. Organisms that pose a risk to food safety, accumulate in the culture media. After the harvest, lettuce cultured in the aquaponic system (AP) was compared with the lettuce cultured in soil (SC). According to the results, AP lettuce was found to have darker colors (Lightness: 56.4 AP, 49.09 SC, p<0.05), harder (Hardness: 209.3 AP, 153.7 SC, p<0.05), and slightly appetizing (Sensory analysis overall liking: 8.4 AP, 7.7 SC) than SC. In conclution, aquaponic systems are much more preferable in terms of sensory quality and consumer preferences than soil-based production systems.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75906051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.01
Can Atacan, F. Düzbastılar
In this study, risk perception and the impact of various environmental factors on accidents involving fishing vessels in small-scale fishing vessels during navigation were examined. Thirty fishing vessel captains from Çeşmealtı and İskele fishing ports evaluated the risks of environmental conditions under different scenarios in the bridge navigation simulator based on the Fine-Kinney risk assessment method. Nonparametric (Mann-Whitney U-test) and parametric tests (Pearson correlation and independent-samples t-test) were performed to analyse other related parameters. The study also conducted a small questionnaire study that included questions such as the number of past accidents by fishermen and the number of engine rudder failures. According to the sum of the fishermen's assessments, reduced visibility was the highest factor increasing the probability and consequences of accidents in sea navigation, while they identified night and heavy weather conditions as the highest factor in port navigation. Fishermen also found navigating their ports safer than sea navigation. There is a significant, positive, and strong correlation between the number of fishermen’s accidents and the number of engine rudder failures (p=0.047, r=0.714), the perception of accident probabilities in port navigating with restricted visibility (p=0.027, r=0.726) and in port navigation at night and in heavy weather (p=0.003, r=0.866). According to the results of the study, using the outcomes of the pre-fishing environmental risk assessment, the competent maritime authorities may be able to take effective measures to prevent the occurrence of serious marine casualties.
{"title":"Determination of risk perception in small-scale fishing and navigation","authors":"Can Atacan, F. Düzbastılar","doi":"10.12714/egejfas.40.1.01","DOIUrl":"https://doi.org/10.12714/egejfas.40.1.01","url":null,"abstract":"In this study, risk perception and the impact of various environmental factors on accidents involving fishing vessels in small-scale fishing vessels during navigation were examined. Thirty fishing vessel captains from Çeşmealtı and İskele fishing ports evaluated the risks of environmental conditions under different scenarios in the bridge navigation simulator based on the Fine-Kinney risk assessment method. Nonparametric (Mann-Whitney U-test) and parametric tests (Pearson correlation and independent-samples t-test) were performed to analyse other related parameters. The study also conducted a small questionnaire study that included questions such as the number of past accidents by fishermen and the number of engine rudder failures. According to the sum of the fishermen's assessments, reduced visibility was the highest factor increasing the probability and consequences of accidents in sea navigation, while they identified night and heavy weather conditions as the highest factor in port navigation. Fishermen also found navigating their ports safer than sea navigation. There is a significant, positive, and strong correlation between the number of fishermen’s accidents and the number of engine rudder failures (p=0.047, r=0.714), the perception of accident probabilities in port navigating with restricted visibility (p=0.027, r=0.726) and in port navigation at night and in heavy weather (p=0.003, r=0.866). According to the results of the study, using the outcomes of the pre-fishing environmental risk assessment, the competent maritime authorities may be able to take effective measures to prevent the occurrence of serious marine casualties.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81774670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.10
Merve Taşkan, M. E. Yonar
In this study, it was aimed to investigate changes in some immunological and antioxidant parameters in scaly carp (Cyprinus carpio) added pollen to their feed in different water temperatures. Fish were stocked to glass aquariums adjusted to 18 °C, 23 °C and 28 °C water temperature. Fish were given diets containing % 2,5 pollen for 14 days. Blood and tissue samples were analysed to determine the immunological parameters and oxidant/antioxidant status. When compared to the control group (23 ºC), a statistically significant increase in the NBT activity of the groups at 18 ºC was observed. Decrease in the NBT activity of the group at 28 º C was statistically insignificant. The total protein and total immunoglobulin levels of the group at 18 º C were decreased, while increase in both parameters at 28 º C was not significant. When compared to the control group (23 ºC), the NBT activity, the total protein and total immunoglobulin levels in the groups that maintained at the same temperature with the control group (23 º C) and applied pollen did not show any statistically significant difference. The tissue MDA levels were increased in the groups at 18 °C and 28 °C when compared to the control group (23 º C). The tissue MDA levels of the groups treated pollen simultaneously with the change in temperature were lower than the groups at 18 °C and 28 °C. The tissue GSH levels and GST activities were decreased in the groups at 18 °C and 28 °C when compared to the control group (23 º C). The tissue GSH levels and GST activities of the groups treated pollen simultaneously with the change in temperature were higher than the groups at 18 °C and 28 °C. In conclusion, stress caused by temperature differences in fish may be prevented by pollen.
{"title":"Investigation of antioxidant effect of pollen in scaly carp (Cyprinus carpio Linnaeus, 1758) in different water temperature","authors":"Merve Taşkan, M. E. Yonar","doi":"10.12714/egejfas.40.1.10","DOIUrl":"https://doi.org/10.12714/egejfas.40.1.10","url":null,"abstract":"In this study, it was aimed to investigate changes in some immunological and antioxidant parameters in scaly carp (Cyprinus carpio) added pollen to their feed in different water temperatures. Fish were stocked to glass aquariums adjusted to 18 °C, 23 °C and 28 °C water temperature. Fish were given diets containing % 2,5 pollen for 14 days. Blood and tissue samples were analysed to determine the immunological parameters and oxidant/antioxidant status. When compared to the control group (23 ºC), a statistically significant increase in the NBT activity of the groups at 18 ºC was observed. Decrease in the NBT activity of the group at 28 º C was statistically insignificant. The total protein and total immunoglobulin levels of the group at 18 º C were decreased, while increase in both parameters at 28 º C was not significant. When compared to the control group (23 ºC), the NBT activity, the total protein and total immunoglobulin levels in the groups that maintained at the same temperature with the control group (23 º C) and applied pollen did not show any statistically significant difference. The tissue MDA levels were increased in the groups at 18 °C and 28 °C when compared to the control group (23 º C). The tissue MDA levels of the groups treated pollen simultaneously with the change in temperature were lower than the groups at 18 °C and 28 °C. The tissue GSH levels and GST activities were decreased in the groups at 18 °C and 28 °C when compared to the control group (23 º C). The tissue GSH levels and GST activities of the groups treated pollen simultaneously with the change in temperature were higher than the groups at 18 °C and 28 °C. In conclusion, stress caused by temperature differences in fish may be prevented by pollen.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75778345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.07
Ahmed Sadeq Jaber Doori, A. Arslan
In this study, a detailed chromosome analysis of the endemic Cappadocian Chub, Squalius cappadocicus in Melendiz Stream (Aksaray) was performed. The standard Giemsa staining, C-banding (CBG and CB-DAPI), and Ag-NOR technique were applied. The diploid chromosome number was 50; its karyotype formula was 14M + 16Sm + 10St + 10A. Heteromorphic sex chromosomes weren’t detected in the karyotype of the studied specimens. The number of all chromosomal arms (NF) was 90. In the standard C-banded and CB-DAPI karyotype of the species, dark C-bands were observed in the centromeric region of some bi-armed and acrocentric chromosomes, while slightly centromeric or pericentromeric C-bands were detected in some chromosomes. Three different active Ag-NORs, which were hemizygous, were detected in all samples examined. Two of these active NORs were detected in the bi-armed and the other in the acrocentric chromosome short arm. The Ag-NOR number of this species was evaluated as a feature that distinguishes it from other Squalius species in Türkiye.
{"title":"Cytogenetic characteristics of endemic Squalius cappadocicus Özuluğ and Freyhof, 2011 in Türkiye","authors":"Ahmed Sadeq Jaber Doori, A. Arslan","doi":"10.12714/egejfas.40.1.07","DOIUrl":"https://doi.org/10.12714/egejfas.40.1.07","url":null,"abstract":"In this study, a detailed chromosome analysis of the endemic Cappadocian Chub, Squalius cappadocicus in Melendiz Stream (Aksaray) was performed. The standard Giemsa staining, C-banding (CBG and CB-DAPI), and Ag-NOR technique were applied. The diploid chromosome number was 50; its karyotype formula was 14M + 16Sm + 10St + 10A. Heteromorphic sex chromosomes weren’t detected in the karyotype of the studied specimens. The number of all chromosomal arms (NF) was 90. In the standard C-banded and CB-DAPI karyotype of the species, dark C-bands were observed in the centromeric region of some bi-armed and acrocentric chromosomes, while slightly centromeric or pericentromeric C-bands were detected in some chromosomes. Three different active Ag-NORs, which were hemizygous, were detected in all samples examined. Two of these active NORs were detected in the bi-armed and the other in the acrocentric chromosome short arm. The Ag-NOR number of this species was evaluated as a feature that distinguishes it from other Squalius species in Türkiye.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81651576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-15DOI: 10.12714/egejfas.40.1.06
Meltem Boyacıoğlu, C. Aygen, M. Karaaslan, Didem Özdemir Mis, Özlem Çakal Arslan
Bakırçay River, one of the important rivers of the Aegean region, has a length of 129 km and an approximate catchment area of 3160 km2. Bakırçay River, which carries agricultural drainage and polluted wastewater with high nitrogen and phosphorus content, is polluted due to domestic and agricultural resources. The water of the Bakırçay River is used for irrigation in agriculture and fishing from the dam lakes on the river for feeding purposes also negatively affects the health of the people in the vicinity. In the Bakırçay River, there is widespread pollution caused by mining areas as well as domestic, industrial and agricultural pollution. For this purpose, the “Algal Growth Inhibition Test, OECD 201” was applied to the water samples obtained from 10 stations on the Bakırçay River. Scenedesmus dimorphus (Turpin) Kützing, 1834 green algae culture, accepted as the primary trophic level representative for the " Algal Growth Inhibition Test, OECD 201" test, one of the short-term phytotoxicity test methods, was used in this test. Water samples were tested at five different dilutions (40, 60, 80, 100 %). To determine the effects on the growth of Scenedesmus dimorphus, the cells were counted by fluorimeter (Turner design) daily at the same time and the test duration was 72 hours. In conclusion, the highest level of toxicity was found at Stations 1, 9, 10,13,14, and 15. The reason for this was that there was a coal facility near Station 9 and that all branches of the Bakırçay River joined near Station15 and then drained into Çandarlı Bay.
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