Emerging Contaminants最新文献

{"title":"Antibiotic resistance genes and microbial diversity in major aquaculture areas of Hainan Island","authors":"Xiaoyu Ren ,&nbsp;Yongqiang Qin ,&nbsp;Yankun Zhang ,&nbsp;Xiaoyu Li ,&nbsp;Yihou Tang ,&nbsp;Xiaoping Diao ,&nbsp;Yuyuan Lin ,&nbsp;Haoyu Qin","doi":"10.1016/j.emcon.2025.100562","DOIUrl":"10.1016/j.emcon.2025.100562","url":null,"abstract":"<div><div>This investigation aims to delineate the spatial distribution, prevalence, and diversity of antibiotic resistance genes (ARGs) within aquatic ecosystems across four coastal aquaculture zones in Hainan Province—Danzhou, Wanning, Wenchang, and Sanya. It further assesses the impact of environmental parameters on ARGs dissemination and microbial community structure. High-throughput sequencing was utilized to analyze water samples from 42 sampling sites, complemented by absolute quantitative PCR to quantify 144 ARGs variants. This approach elucidated correlations between ARGs and microbial assemblages, as well as the influence of environmental factors on these interactions. Results indicated that the number of ARGs detected per site ranged from 61 to 123, with Danzhou exhibiting 90 to 123, Wanning 74 to 123, Wenchang 68 to 122, and Sanya 61 to 109 ARGs. Notably, 16 sampling points harbored over 110 ARGs. Quantitative analysis revealed ARG concentrations ranging from 16,167 to 1,784,618 copies/ng. Co-occurrence network analysis demonstrated that most ARGs were associated with MGEs, 16S rRNA genes, transposons, and integrons, indicating these elements' critical role in horizontal gene transfer. Microbial community profiling identified 27,050 operational taxonomic units (OTUs) across 72 phyla, 153 classes, 371 orders, 459 families, and 924 genera, with <em>Proteobacteria</em>, <em>Bacteroidota</em>, <em>Actinobacteriota</em>, <em>Cyanobacteria</em>, and <em>Planctomycetota</em> as dominant taxa. Genus-level analysis revealed significant variations in the abundance of taxa such as <em>Cyanobium_PCC-6307</em>, <em>Candidatus_Actinomarina</em>, and <em>HIMB11</em> across sampling sites. Environmental parameters, including pH, dissolved oxygen (DO), specific conductance (SPC), salinity (SAL), temperature (T), and pressure (kPa), showed strong correlations with ARG distribution and microbial community composition. Region-specific co-linearity networks indicated distinct relationships between microbial taxa and ARGs; notably, Danzhou and Wanning exhibited strong correlations with over 20 ARGs per genus, whereas Wenchang displayed a more complex association pattern, suggesting a multifaceted role in ARG transmission pathways. Overall, the findings suggest that environmental factors and microbial hosts significantly influence ARG prevalence in Hainan's coastal waters, with regional variations in microbial-ARG interactions, particularly in Wenchang, indicating heightened transmission risks. This study offers vital insights for sustainable aquaculture practices and informs regional regulatory strategies for aquaculture management.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100562"},"PeriodicalIF":6.9,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interfacial interaction mechanisms between sediment-microplastics heterogeneous particulate matter (HPM) and cadmium (Cd) in saline environment","authors":"Yun Li ,&nbsp;Zhihe Chen","doi":"10.1016/j.emcon.2025.100565","DOIUrl":"10.1016/j.emcon.2025.100565","url":null,"abstract":"<div><div>As microplastics enter aquatic environments, it remains unclear whether interfacial interactions between heterogeneous particulate matter (HPM, co-existing sediment and microplastics) and heavy metals in saline waters follow the same mechanisms as single particles, and how these interactions affect metal mobility and ecological risk. This study elucidates the interfacial mechanisms between HPM and cadmium (Cd) under various salinity conditions, highlighting the critical role of microplastics within HPM. Results show that: (1) 5 PSU and 15 PSU serve as critical salinity thresholds influencing interfacial interactions–low salinity (&lt;5 PSU) enhances surface properties, promoting rapid initial adsorption (0–30 min) but limited retention, whereas high salinity (&gt;15 PSU) significantly inhibits Cd adsorption; (2) Microplastics increase the salinity sensitivity of aquatic systems by modifying interfacial interaction to enhance the inhibitory effect, with maximum inhibition rate (IR = 41.29 %) observed at 7.5 PSU; (3) Salinity influence function (<em>F</em>_s), according to the salinity divergence coefficient (SDC), accurately characterizes the salinity effect and the microplastics contribution to Cd adsorption on HPM (<em>R</em>² = 0.92), supporting robust predictors of heavy metal fate. These findings offer theoretical insight into heavy metal transport in salt-affected aquatic environments.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100565"},"PeriodicalIF":6.9,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of microplastic degradability and concentrations on antibiotic resistance genes between soil and phyllosphere","authors":"Jianzhong Xu ,&nbsp;Lingyue Lv ,&nbsp;Zheyu Li ,&nbsp;Xunqiang Mo ,&nbsp;Mengxuan He ,&nbsp;Yingchao Lin","doi":"10.1016/j.emcon.2025.100564","DOIUrl":"10.1016/j.emcon.2025.100564","url":null,"abstract":"<div><div>Microplastics have emerged as significant environmental pollutants and notably facilitated the spread of antibiotic resistance genes (ARGs); however, their impacts and underlying mechanisms remain poorly understood. So, this study investigated the effects of different types of microplastics (biodegradable microplastics; PGA and non-degradable microplastics; HDPE) and their concentrations (0.5 %, 1.0 %, and 1.5 % w/w) on ARGs distribution and transfer between soil and the phyllosphere, utilizing a greenhouse germination experiment. The results demonstrated that microplastic addition altered the characteristics of ARGs in both soil and phyllosphere. PGA, in particular, had a more pronounced effect on ARGs abundance in the phyllosphere. In soil, ARGs abundance were jointly regulated by both microplastic type and concentration: PGA exerted a stronger influence at lower concentrations, while HDPE had a greater effect at higher concentrations. Regardless of type or concentration, microplastic addition reduced microbial network modularity, leading to substantial shifts in ARGs community structure in both soil and phyllosphere. Notably, microplastic addition at 1 % concentration yielded the highest ARGs diversity in soil. Structural equation model revealed that microplastic types and concentrations influenced ARGs transfer via distinct pathways by changing soil physicochemical properties and microbial diversity. Specifically, PGA increased soil electrical conductivity (EC) and dissolved organic carbon (DOC) to influence ARGs, while HDPE primarily affected ARGs through alterations in soil pH, available phosphorus (AP), and available potassium (AK). These findings can offer important insights into the mechanisms by which microplastics influence ARGs dissemination in terrestrial ecosystems.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100564"},"PeriodicalIF":6.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PPARα-dependent lipid metabolic disruption mediates triphenyl phosphate–induced hepato- and neurotoxicity in zebrafish","authors":"Congying Luo ,&nbsp;Qiong Zhang ,&nbsp;Han Xie ,&nbsp;Dinghui Wang ,&nbsp;Xiaoling Shi ,&nbsp;Yanhong Huang ,&nbsp;Yimin Zhang ,&nbsp;Kusheng Wu","doi":"10.1016/j.emcon.2025.100561","DOIUrl":"10.1016/j.emcon.2025.100561","url":null,"abstract":"<div><div>The extensive use of triphenyl phosphate (TPhP) has resulted in its ubiquity in aquatic ecosystems, raising significant concerns about its escalating risks to aquatic organisms. In this study, we employed adult and larval zebrafish (<em>Danio rerio</em>) as model organisms to assess the adverse effects of TPhP exposure on aquatic life. The objectives were to evaluate TPhP-induced hepatotoxicity and neurotoxicity and the role of lipid metabolism in mediating these toxic effects. The results demonstrated that TPhP exposure led to alterations in body weight, length, and body mass index (BMI), along with changes in brain and liver somatic indices in zebrafish. Through comprehensive analyses of histopathological changes, transcriptional profiles, biochemical markers, and neurobehavioral assays, TPhP was shown to induce both morphological and functional impairments in the brain and liver. In TPhP-exposed larvae, reduced tissue integrity and altered spatial distribution of brain and hepatic tissues were observed. Notably, changes in the expression of neurodevelopmental and lipid metabolism-related genes in larvae mirrored those detected in adult zebrafish, indicating conserved toxic mechanisms across life stages. Mechanistically, TPhP exposure induced oxidative stress in both brain and liver tissues and suppressed the expression of PPARα, as well as its downstream genes involved in fatty acid β-oxidation and lipid homeostasis. Consequently, hepatic triglyceride (TG) and total cholesterol (T-CHO) levels were significantly decreased, whereas free fatty acid (FFA) content was elevated. Thus, TPhP inhibits PPARα signaling via oxidative stress, leading to lipid metabolic dysfunction and subsequent damage to neural and hepatic tissues. This study provides robust evidence of TPhP-induced hepatotoxicity and neurotoxicity in zebrafish, and elucidates the critical role of PPARα-mediated disruption of lipid metabolism in these toxic pathways, thereby enhancing our understanding of the ecological risks posed by organophosphate flame retardants.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100561"},"PeriodicalIF":6.9,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro metabolites characterization of 1,3-diphenylguanidine and 1,3-di-o-tolylguanidine by high-resolution mass spectrometry and urinary profiling","authors":"Andrea Estévez-Danta,&nbsp;Iago Riveiro,&nbsp;María Lage-Díaz,&nbsp;José Benito Quintana,&nbsp;Rosa Montes,&nbsp;Rosario Rodil","doi":"10.1016/j.emcon.2025.100551","DOIUrl":"10.1016/j.emcon.2025.100551","url":null,"abstract":"<div><div>The discovery of the hazardous effects associated with the polymer additives 1,3-diphenyl guanidine (DPG) and 1,3-di-o-tolylguanidine (DTG) has prompted the need for biomonitoring studies to detect human exposure. However, limited information is available about their metabolism. To address this gap, this study investigates the Phase I and II <em>in-vitro</em> biotransformation of both chemicals using human liver microsomes and cytosol. The samples were analyzed using liquid chromatography coupled to high resolution-(tandem) mass spectrometry through suspect (of <em>in-silico</em> predicted metabolites) and non-target screening. The analysis revealed four Phase I and two Phase II metabolic products for both DPG and DTG. Hydroxylation of the benzene ring led to the tentative identification of mono- and di-hydroxylated metabolites. Subsequent Phase I deamination followed by oxidation resulted in the formation of hydroxy-phenylurea and an intramolecular cyclization resulted in the formation of hydroxy-cyclic products. Furthermore, N-glucuronidation and O-glucuronidation products were identified for the first time. After performing urinalysis, DPG and DTG could be quantified in the 0.02–0.23 μg L⁻¹ range, and DPG-227 (mono-hydroxylated DPG) was estimated to be present at ca. 0.01–0.10 μg L⁻¹ range, using DPG response as quantification surrogate. Finally, toxicity assessment using an <em>in-silico</em> tool indicated the need to consider these human metabolites in (eco)toxicological assessments, as they may have the same or even greater effects on humans and the environment.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100551"},"PeriodicalIF":6.9,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144926053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the greenhouse: The spread of plastic pollution from an intensively cultivated agricultural area","authors":"Marina Nuñez-Rubio ,&nbsp;Carlos Edo ,&nbsp;Virginia Gálvez-Blanca ,&nbsp;Juan Manuel Valenzuela-Lázaro ,&nbsp;Gerardo Pulido-Reyes ,&nbsp;Miguel González-Pleiter ,&nbsp;Luis Fernández-García-del-Rincón ,&nbsp;Francisco Leganés ,&nbsp;Francisca Fernández-Piñas ,&nbsp;Roberto Rosal","doi":"10.1016/j.emcon.2025.100560","DOIUrl":"10.1016/j.emcon.2025.100560","url":null,"abstract":"<div><div>Among the many sources of plastic contamination, greenhouse agriculture is a major contributor due to its intensive use of plastic materials. This study examines plastic waste in one of the world's leading greenhouse farming regions. The research included both abiotic (soil, freshwater, seawater, and air) and biotic compartments (invertebrates, herbivores, fish and predators) as well as the presence of bacterial and fungal pathogens colonizing agricultural plastics. Plastics were identified in all sampled compartments, including the nearshore seawater. The highest concentrations were recorded along the coastline, where 1397 plastic items representing 15 different polymers were found with a median concentration of 1.92 (1.73–2.13) items/m² or 3.05 (2.29–3.81) g/m², the boundaries representing interquartile ranges. Most of the polymers identified across all compartments matched those found in agricultural operations in terms of morphology, type, and colour. Notably, high plastic concentration appeared in protected areas, indicating that legal protection alone does not prevent pollution. Greenhouse plastics were especially prevalent in the protected zones near the greenhouse area, rather than in the nearby urban zones, which can be explained by the proximity of agricultural exploitations. We estimated that approximately 290 kg of plastic, including 80 kg of polyethylene, were dispersed along the coast, a figure representing about one-tenth of the plastic waste generated annually in the production zone. Our results also revealed that agricultural plastic waste may act as vector and reservoir of bacterial and fungal pathogens, promoting their spread even into protected environments.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100560"},"PeriodicalIF":6.9,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfiber release during apparel manufacturing process – A greater concern than domestic laundering","authors":"S. Raja Balasaraswathi,&nbsp;R. Rathinamoorthy","doi":"10.1016/j.emcon.2025.100559","DOIUrl":"10.1016/j.emcon.2025.100559","url":null,"abstract":"<div><div>Microfibers released from textile materials have recently become a pressing concern as one of the most significant anthropogenic pollutants in recent times. Although domestic laundry has been reported as a major source of microfiber release, researchers stress the potential of manufacturing stage to contribute equally. However, limited research has been conducted on the manufacturing stage. To bridge this gap, the current study aimed to analyze microfiber release from different knitted fabrics during the cutting process in the apparel manufacturing industry. Microfibers released during cutting were collected using the atmospheric deposition method both inside and outside the industry. The findings revealed that the cutting process caused an average microfiber deposition of 2.86 × 10⁶ ± 6.02 × 10⁵ microfibers per square meter of cutting floor per day. A strong linear relationship was observed between the length of the fabric been cut and microfiber deposition (r = 0.96). Regarding fabric properties, strong positive correlations (r = 0.80 to 0.99) were noted with stitch density, thickness, areal density, and yarn count, indicating that increase in these parameters led to increased microfiber emissions. Conversely, a negative correlation was reported for loop length (r = −0.79), and an insignificant correlation was found with the tightness factor (r = 0.49). The majority of the released fibers (55.84 %) measured between 300 and 800 μm in length. The results showed that fabrics with compact structures released more short microfibers than those with loose structures. The study's findings indicated that cutting a medium-sized T-shirt can release up to 1.09 × 10⁶ microfibers, which is approximately 50 times higher than that released during laundry, with 40–70 % of the fibers transported outside the factory. With this alarming quantity, future research should focus on understanding the occupational hazards of cutting operators exposed to large quantities of microfibers. Moreover, studies should focus on developing cost-effective alternative cutting methods and microfiber-capturing systems that can be implemented in the industry to control the release of microfibers into the external environment.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100559"},"PeriodicalIF":6.9,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating contaminants of emerging concern (CECs) in the Venice Lagoon: A suspect screening approach for the analysis of water and sediment contamination","authors":"P.P. Guolo ,&nbsp;L. Calgaro ,&nbsp;A. Bonetto ,&nbsp;Q. Xu ,&nbsp;J. Lu ,&nbsp;A. Marcomini","doi":"10.1016/j.emcon.2025.100557","DOIUrl":"10.1016/j.emcon.2025.100557","url":null,"abstract":"<div><div>The presence of Contaminants of Emerging Concern (CECs) in the environment poses significant concern, yet their occurrence, sources, and fate remain not fully understood. This study provides the first investigation of a broad range of CECs, including active pharmaceutical ingredients (APIs), plant protection products (PPPs), and personal care products (PCPs), across raw and treated wastewater, surface water, and sediment in the Venice Lagoon and selected tributaries. Using a suspect screening analysis methodology based on high-resolution mass spectrometry (HRMS), 14 sampling stations were selected to identify contamination patterns and potential emission sources. APIs were the most frequently detected class of compounds (150 compounds), with flecainide, harman, carbamazepine, and venlafaxine found in more than 80 % of sampling stations. Analgesics were the dominant therapeutic class, followed by antidepressants and cardiovascular drugs. Among PCPs (17 compounds), icaridin, and climbazole were the most prevalent, detected at 11 and 9 sampling stations, respectively. PPPs (26 compounds) were also detected, with diuron being the most widespread as it was found at 10 sampling stations. To the best of the authors' knowledge, 92 compounds (68 APIs, 17 PCPs, and 7 PPPs) were qualitatively identified for the first time in the Venice Lagoon, although many of them are known to occur in natural waters worldwide.</div><div>The results highlight rivers and treated wastewater as major contributors to CEC emissions in the lagoon. These findings offer valuable insights for future research and targeted monitoring strategies, advancing the understanding of unmonitored contaminants in vulnerable aquatic environments.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100557"},"PeriodicalIF":6.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Holographic imaging and machine learning for microplastic size and shape analysis in water","authors":"Ismaila Abimbola ,&nbsp;Thangavel Thevar ,&nbsp;Marion McAfee ,&nbsp;Leo Creedon ,&nbsp;Hanieh Khosravi ,&nbsp;Salem Gharbia","doi":"10.1016/j.emcon.2025.100558","DOIUrl":"10.1016/j.emcon.2025.100558","url":null,"abstract":"<div><div>Microplastics are a growing global concern, particularly in drinking water, due to their potential negative impacts on human health. To effectively monitor, quantify and understand the sources and implications of microplastics in water, it is critical to identify their physical and chemical properties. However, existing laboratory-based methods popularly used for characterising microplastics have several limitations. Using a novel method, this study explored the feasibility of quantifying the physical properties of microplastics in water. Specifically, we utilised a portable holographic camera to record digital holograms of commercial microplastics floating in water. Furthermore, we developed a simple Python algorithm to determine the size of the microplastics from the particle images. This study also evaluated and compared the performance of two deep-learning architectures, MobileNetV2 and ResNet101, in classifying the shapes of the microplastic particles into spherical and hemispherical shapes. Findings from this study demonstrate the capability of the proposed holographic system to rapidly and automatically produce particle images of microplastics while simultaneously measuring their sizes. Performance metrics, including accuracy, precision, recall, F1 score, confusion matrix and training time, showed that MobileNetV2 achieved the best performance despite being a more lightweight model with fewer parameters than ResNet101. Therefore, MobileNetV2 was recommended for classifying the shapes of microplastics from particle images. The time and cost-effectiveness of the proposed digital holographic method make it suitable for large-scale monitoring of microplastics in water. This will be significant in identifying the sources, understanding their behaviour and reducing the associated health risks to humans.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100558"},"PeriodicalIF":6.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PFOA promotes hepatocellular carcinoma cell progression by suppressing ferroptosis via the ATF4/SLC7A11/PDK4 axis","authors":"Wenli Hu ,&nbsp;Yu Chen ,&nbsp;Xiaohang Guo ,&nbsp;Zhuang Zhang ,&nbsp;Ping Zhu ,&nbsp;Xiushan Wu ,&nbsp;Xin Li","doi":"10.1016/j.emcon.2025.100556","DOIUrl":"10.1016/j.emcon.2025.100556","url":null,"abstract":"<div><div>Per- and polyfluoroalkyl substances (PFASs) are persistent pollutants with emerging hepatotoxic and tumor-promoting concerns. Differences in effects between long- and short-chain PFAS on liver tumor progression remain unclear. We examined transcriptomic and functional effects of PFOA (long-chain), GenX (short-chain), and their 1:1 mixture on HepG2 cells exposed to a nominal total concentration of 0.02 mg/L. Using RNA-Seq, qPCR, and assays for proliferation, migration and invasion, we found that PFOA induced the largest number of differentially expressed genes and markedly promoted proliferation, migration and invasion via activation of TNF and insulin-resistance pathways. PFOA also suppressed ferroptosis through upregulation of the ATF4/SLC7A11/PDK4 axis, supported by decreased BODIPY-C11 lipid peroxidation and lower labile Fe²⁺. GenX showed substantially weaker effects, and the mixture produced intermediate responses consistent with a concentration-addition (dose-dilution) explanation. These findings indicate that PFOA promotes malignant phenotypes of existing liver cancer cells in vitro rather than demonstrating de-novo carcinogenesis, and highlight the need to evaluate tumor-promoting and hepatotoxic effects of PFAS under both single-compound and mixed exposures.</div></div>","PeriodicalId":11539,"journal":{"name":"Emerging Contaminants","volume":"11 4","pages":"Article 100556"},"PeriodicalIF":6.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}