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Sugar Cane and Sugar Research at National Cereals Research Institute, Badeggi, Nigeria: Time for Collaboration with Egypt’s Sugar Groups 尼日利亚badegi国家谷物研究所的甘蔗和糖研究:是时候与埃及糖业集团合作了
Pub Date : 2019-06-01 DOI: 10.21608/esugj.2019.223907
Akammaa Wada, A. Gbabo, A. Umar
National Cereals Research Institute situated on Lat 09045' N, Long 6007'E at an Altitude of 70.57m above sea level has the national mandate for genetic improvement of sugar cane and five other crops. Thus, National Cereals Research Institute, Badeggi has been involved in Research and Development (R & D) of sugar cane for the past 3 and 4 decades respectively. During this period, seven indigenously bred varieties have been released for use by the Nigerian sugar industries. The research efforts on sugar have also witnessed the development of 10 tons per day (10tcd) brown sugar processing technology for cottage level sugar production. In striving to achieve these novel technologies, various stakeholders were collaborated with spanning from the Federal Government, Agencies of Governments at the Federal, state and local levels and the universities with little or no input from the Nigerian Sugar Industry. Due to some encountered deficiency in key areas for sugar cane and sugar research and production, the Institute finds imperative to seek worthy collaborators in Egypt's sugar groups in biotechnology and sugar technologies for the development of suitable varieties foe sugar and ethanol production and the time for this synergy is now. This paper highlights what NCRI has been able to archive in the past decades on sugarcanes and sugar research and areas needing urgent collaboration with relevant Egypt technocrat in sugar cane and sugar.
国家谷物研究所位于北纬09045度,东经6007度,海拔70.57米,是甘蔗和其他五种作物遗传改良的国家任务。因此,巴德吉国家谷物研究所在过去的30年和40年里分别参与了甘蔗的研究和开发。在此期间,已经发布了7种当地培育的品种供尼日利亚制糖业使用。对糖的研究也见证了每天10吨(10tcd)红糖加工技术的发展,用于家庭糖生产。在努力实现这些新技术的过程中,与联邦政府、联邦、州和地方各级政府机构以及大学等各利益攸关方合作,尼日利亚制糖业很少或根本没有投入。由于在甘蔗和糖的研究和生产的关键领域遇到了一些不足,研究所认为必须在埃及的糖集团中寻找生物技术和糖技术方面有价值的合作者,以开发适合糖和乙醇生产的品种,现在是进行这种协同作用的时候了。本文重点介绍了NCRI在过去几十年中关于甘蔗和糖的研究以及需要与埃及相关技术官僚在甘蔗和糖方面进行紧急合作的领域。
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引用次数: 0
Isolation and partial purification of invertase from different baker's and distillery Saccharomyces cerevisiae 不同酿酒酵母转化酶的分离与部分纯化
Pub Date : 2018-12-01 DOI: 10.21608/esugj.2018.228290
A. Zohri, Abdel-Al Gaber, Osama Ahmed, Ahmed Mohammed
Invertase enzyme from different commercial kinds of Baker’s and distillery Saccharomyces cerevisiae was isolated and partially purified as a crude enzyme by salt stress process and ethanol precipitation. The lyophilization takes place for the enzyme isolated from activated distillery S. cerevisiae. The activities of partially purified invertase isolated from the different five baker yeasts under study ranged between 975 and 1801 IU/ml/min. While the activities of enzymes isolated from distillery yeasts before and after activation were 120 and 1100 IU/ml/min. The activity of lyophilized partially purified powder of enzyme isolated from activated distillery yeast was19300 U/g. The Km and Vmax values of the enzyme isolated from activated distillery yeast were 7.56 mg/ml and 294.12 IU/ml/min, respectively. These results were compared to those values of invertase isolated from Egyptian active dry Baker’s yeast and the commercial invertase data sheet with a sample of sweetase 20 p enzyme obtained from Germany stern enzyme company. The results recorded in this paper clearly appeared that the active powder invertase could be obtained from activated distillery yeast by the easy and inexpensive method.
采用盐胁迫法和乙醇沉淀法,从不同商业品种的酿酒酵母中分离出转化酶,并将其作为粗酶进行部分纯化。对从活化的酿酒酵母中分离出来的酶进行冻干。从5种酵母中分离得到的部分纯化转化酶活性在975 ~ 1801 IU/ml/min之间。而酵母菌活化前后的酶活分别为120和1100 IU/ml/min。活性酵母菌分离的部分纯化酶冻干粉的活性为19300 U/g。该酶的Km和Vmax分别为7.56 mg/ml和294.12 IU/ml/min。这些结果与从埃及活性干贝克酵母中分离的转化酶的值和从德国斯特恩酶公司获得的糖酶20p酶样品的商业转化酶数据表进行了比较。本文所记录的结果清楚地表明,从活化的酿酒酵母中提取活性粉末转化酶是一种简单、廉价的方法。
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引用次数: 1
Evaluation of sugarcane genotypes under different row spacing 不同行距下甘蔗基因型的评价
Pub Date : 2018-12-01 DOI: 10.21608/esugj.2018.228294
A. Ahmed, A. El-Taib, A. Eanar
The present study was carried out at El-Mattana Agricultural Research Station (Latitude of 25.17 o N and longitude of 32.33 o E), Luxor Governorate, Egypt during the 2014/2015 and 2015/2016 growing seasons to find out the suitable row spacing (80, 100 and 120 cm) on yield and quality of two promising sugarcane varieties viz. G.2003-47 and G.2003- 49 compared with the commercial variety (G.T. 54-9). The results indicated significant differences among tested genotypes in stalk height, cane yield in the plant cane and first ratoon crops, while stalk diameter and sugar yield exhibited significant differences only in the first ratoon. Row spacing had a significant effect on the number of millable cane / m 2 , cane and sugar yields /fed in the plant cane and first ratoon crops, while it had a significant effect on stalk height only in plant cane crop. The row spacing of 120 cm exerted the highest number of millable cane / m 2 , stalk height, and sugar yield in plant cane and first ratoon crops, as well as stalk diameter, cane yield, Brix, richness and reducing sugars in plant cane only. Moreover, the check cultivar recorded the highest mean value of sugar yield in the first ratoon crop, when planted at 120 cm row spacing. On the other hand, the 80 cm inter row-spacing gave the highest mean values of purity and sugar recovery% in both plant cane and first ratoon crops and Brix, sucrose and richness in first ratoon only. The significant linear effects were found for the number of millable cane / m 2 , stalk height, cane yield, sugar yield and reducing sugar with the rate of row spacing.
本研究于2014/2015和2015/2016生长季在埃及卢克索省El-Mattana农业研究站(纬度25.17 o N,经度32.33 o E)进行,以比较G.2003-47和G.2003- 49两个有前景的甘蔗品种(G.T. 54-9)的产量和品质,找出合适的行距(80、100和120 cm)。结果表明,不同基因型间甘蔗植株的茎高和甘蔗产量存在显著差异,而茎粗和糖产量仅在第一代作物中存在显著差异。行距对甘蔗和第一代再生作物的可蔗数/ m2、甘蔗产量和食糖产量均有显著影响,而对甘蔗的茎高有显著影响。行距为120 cm时,蔗株和初生作物的可蔗数、茎高和糖产量最高,蔗株的茎粗、甘蔗产量、糖度、丰度和还原糖含量最高。此外,对照品种在行距120 cm时,在第一次再生作物中平均糖产量最高。另一方面,行距为80 cm时,甘蔗和第一代作物的纯度和糖回收率平均值最高,而糖度、蔗糖和丰富度仅在第一代作物中最高。可蔗数/ m2、茎高、甘蔗产量、糖产量、还原糖产量与行距率呈显著的线性关系。
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引用次数: 0
Effect of Hydroxyapatite Nanoparticles on the Behavior of Calcium, Silicate, and Phosphate in Cane Juice Treatment 羟基磷灰石纳米颗粒对甘蔗汁处理中钙、硅酸盐和磷酸盐行为的影响
Pub Date : 2018-12-01 DOI: 10.21608/esugj.2018.228103
T. Salaheldin, Mohamed Mahmoud Abd El Wahab, A. Awadalla, Ahmed N. Gad
Taher A. Salaheldin , Mohamed Mahmoud Abd El Wahab Hoda, A. Awadalla, and Ahmed Nasrallah Gad Nano Technology Characterization Lab., British university in Egypt. Nanotechnology& Advanced Materials Central Lab., Agricultural Research Center. Sugar Technology Research Institute,Assiut University, Egypt. Research & Development Center, ESIIC. ______________________________________________________________
Taher A. Salaheldin, Mohamed Mahmoud Abd El Wahab Hoda, A. Awadalla和Ahmed Nasrallah Gad纳米技术表征实验室。在埃及的英国大学。纳米技术与先进材料中心实验室。农业研究中心。埃及阿西尤特大学糖业技术研究所。ESIIC研发中心。______________________________________________________________
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引用次数: 0
Potentiality of Some Yeast Isolates for Electricity Generation from Sugarcane Molasses 一些酵母菌分离株用于甘蔗糖蜜发电的潜力
Pub Date : 2018-12-01 DOI: 10.21608/esugj.2018.228116
A. Zohri, S. Hassan, Rehab M. F. Kassim
Microbial fuel cells (MFCs) are considered simple and robust technology in the field of wastewater treatment as well as electricity generation. The middle-term application can be foreseen at market value prices. This technology might become certified as a prospective new technology for the conversion of organic wastes to electricity. In the current study, a total of 40 yeast isolates were collected from 20 samples of sugarcane molasses. Twenty-one yeast isolates were examined for their potential to produce electricity from sugarcane molasses. All isolates had the ability to produce electricity. Four of them (isolates number: MY 9, MY10, MY18 and MY19 were considered as high producers. They yielded power densities of 59.858, 61.25, 52.8125 and 47.432 mW/m 2 , respectively. Yeast isolate MY10 from sugarcane molasses showed the highest power density (P) (61.25 mW/m 2 ), highest current (I) (0.175mA/m 2 ) and highest voltage (V) (350mV/m 2 ). This isolate was identified based on 18S rRNA sequences as Meyerozyma guilliermondii and deposited in the Gene bank nucleotide sequence database under accession numbers KY624417.
微生物燃料电池(mfc)被认为是污水处理和发电领域中简单而强大的技术。中期应用可按市场价值价格预测。这项技术可能被证明是一项有前途的有机废物转化为电能的新技术。本研究从20份甘蔗糖蜜样品中分离到40株酵母菌。研究了21株酵母菌从甘蔗糖蜜中发电的潜力。所有的分离株都有发电的能力。其中4株(分离株数:my9、MY10、MY18和MY19)为高产菌株。它们的功率密度分别为59.858、61.25、52.8125和47.432 mW/ m2。甘蔗糖蜜酵母分离物MY10表现出最高功率密度(P) (61.25 mW/ m2)、最高电流(I) (0.175mA/ m2)和最高电压(V) (350mV/ m2)。该分离物经18S rRNA序列鉴定为吉列蒙氏沼菌,并存入基因库核苷酸序列数据库,登录号为KY624417。
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引用次数: 1
A Study on the factors affecting biomass formation by a highly kojic acid producer fungal isolate from sugarcane molasses 甘蔗糖蜜中高曲酸产菌分离株生物量影响因素的研究
Pub Date : 2018-12-01 DOI: 10.21608/esugj.2018.228291
A. Zohri, G. Mahmoud, Nermien Sadik, Radwa A. Hanafy
Nowadays, microbial production of natural products represents a hot spot point in our environment and becomes an alternative way to chemical synthetic. In our research, Aspergillus flavus No . 3 shows great power in both biomass and kojic acid production. Plackett–Burman design utilize Egyptian sugarcane molasses as a sole carbon source giving kojic acid production of 0.82 (predicted 1.04) g/l to 24.65 (predicted 23.74) g/l, consuming sugar at 27.33 (predicted 26.96) % to 89.87 (predicted 87.46) % and forming dry biomass between 3.6 (predicted 3.8) g/l and 28.2 (predicted 28.05) g/l. The maximum kojic acid (24.65 g/l) and biomass value (28.2 g/l) obtained at 25°C; 9, 5 days of incubation, pH 3, 5; 0.5%, 2% inoculum size and the shaking rate at 150 rpm using fermentation medium (g/l) of sugarcane molasses, 60; yeast extract, 7, 3; KH 2 PO 4, 2; ZnSO 4 .7H 2 O, 0.001 and MgSO 4 .7H 2 O, 1, respectively.
微生物生产天然产物是当今环境研究的热点,已成为化学合成的替代途径。在我们的研究中,黄曲霉号。3在生物质和曲酸生产中都表现出巨大的动力。Plackett-Burman设计利用埃及甘蔗糖蜜作为唯一的碳源,使曲酸产量为0.82(预测1.04)g/l至24.65(预测23.74)g/l,糖消耗量为27.33(预测26.96)%至89.87(预测87.46)%,形成的干生物量在3.6(预测3.8)g/l至28.2(预测28.05)g/l之间。25℃时获得最大的曲酸(24.65 g/l)和生物量值(28.2 g/l);9,5天孵育,pH为3,5;0.5%, 2%接种量,摇速150 RPM,使用发酵培养基(g/l)甘蔗糖蜜,60;酵母浸膏,7,3;Kh 2 Po 4,2;znso4.7 h2o, 0.001和mgso4.7 h2o, 1。
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引用次数: 1
Application of Nano-Hydroxyapatite in Sugarcane Juice Clarification 纳米羟基磷灰石在甘蔗汁澄清中的应用
Pub Date : 2018-12-01 DOI: 10.21608/esugj.2018.228094
T. Salaheldin, M. Abd El Wahab, H. Awadalla, A. Gad
______________________________________________________________ Abstract In this study, the application of nano-hydroxyapatite (NHA) in the clarification of mixed cane juice "MJ” was investigated via batch experiments on a pilot scale. Control points (pH, doses, retention time, color, and turbidity) of this process were evaluated compared with the traditional sulfitation process. The results were evaluated using a statistical T-test. The results showed that the most proper pH, doses and retention time are 7.5, 300 ppm, and 1.5 hr, respectively. Since, applying such values led to a significant reduction in turbidity and color of clear juice (55 % and 19 % respectively) compared with the traditional sulfitation and phosphation process. These results mean a predicted soft scale formed in the evaporators, the lower color of produced sugar, higher sugar recovery, removing the cost of sulfur, and minimizing the cost of calcium oxide to half. The results were discussed via the properties of hydroxyapatite and its highest surface area that adsorbs denatured proteins, prevents the formation of melanoidin coloring compounds and promotes clarification efficiency.
摘要本研究通过中试批量实验,研究了纳米羟基磷灰石(NHA)在混合蔗汁“MJ”澄清中的应用。对该工艺的控制点(pH、剂量、保留时间、颜色和浊度)与传统磺化工艺进行了比较。采用统计t检验对结果进行评价。结果表明,最适宜的pH为7.5,剂量为300 ppm,滞留时间为1.5 hr。因为,与传统的亚硫酸和磷化工艺相比,应用这些值导致透明果汁的浊度和颜色显著降低(分别为55%和19%)。这些结果意味着在蒸发器中形成了预期的软垢,产生的糖的颜色更低,糖的回收率更高,去除硫的成本,并将氧化钙的成本降至一半。结果通过羟基磷灰石的性质及其吸附变性蛋白质的最大表面积,防止类黑素着色化合物的形成和提高澄清效率进行了讨论。
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引用次数: 0
Effect of boron concentrations and application time on the productivity and quality of some sugar beet cultivars 硼浓度和施用时间对部分甜菜品种产量和品质的影响
Pub Date : 2018-12-01 DOI: 10.21608/esugj.2018.223948
Elmahdy Teama, E. Ali, F. Fathy, Rasha Mustafa
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引用次数: 0
Study on photogeneration of hydrogen peroxide over ag/tio2 catalysts in water 过氧化氢在ag/tio2催化剂上在水中光生成的研究
Pub Date : 2018-12-01 DOI: 10.21608/esugj.2018.228293
A. Said, R. Gabr, Bahaa E. Ali, A. El-Naggar
In the present study, catalytic photogeneration of hydrogen peroxide has been carried out in the presence of Ag-TiO2 catalysts by using a batch reactor with a UV (36 watts) lamp. A series of Ag-TiO2 catalysts (0.25-5%) was synthesized by the wet impregnation method and with a doping content of up to 5.0 wt% Ag at different calcination temperatures (200, 300, 400 and 500°C). The physic-chemical characteristics of the synthesized catalysts were characterized by powder X-ray diffraction (XRD), and Fourier transforms infrared spectroscopy (FT-IR). The photocatalytic activity of Ag-TiO2 was evaluated in the H2O2 photogeneration in an aqueous solution in presence of formic acid under UV light illumination. The concentration of photogenerated H2O2 was estimated iodometrically. The results revealed that the yield of H2O2 photogeneration at optimum reaction conditions (176 ppm) was achieved.
在本研究中,在Ag-TiO2催化剂的存在下,利用间歇式反应器和UV灯(36瓦)进行了过氧化氢的催化光生成。采用湿浸渍法,在不同焙烧温度(200、300、400、500℃)下合成了一系列Ag- tio2催化剂(0.25 ~ 5%),Ag掺杂量高达5.0 wt%。采用粉末x射线衍射(XRD)和傅里叶变换红外光谱(FT-IR)对合成催化剂的理化性质进行了表征。在紫外光照射下,测定了Ag-TiO2在甲酸存在下的H2O2光催化活性。用碘量法测定了光生成H2O2的浓度。结果表明,在最佳反应条件(176 ppm)下,H2O2产率达到最高。
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引用次数: 0
Cleaning of clogging central tube in juice clarifiers 果汁澄清器中心管堵塞的清洗
Pub Date : 2018-06-01 DOI: 10.21608/esugj.2018.228400
A. K. Kamal El‐dean, M. Abd El-Wahab, Hashim Yassin, Muhammad Abdullah
This research aims to remove the solid sediments that completely blocked the central distribution pipe in the juice clarifiers. These sediments can't be removed by traditional methods such as crushing or heating, due to the special condition of the pipe inside the clarifier, its narrow diameter and vertical position. Therefore, chemical methods were used to solve this problem. A new technique was used in this process through a mixture of sodium hydroxide and hydrogen peroxide solution. There are many experiments and attempts to find the most suitable way of addition and also the final required concentrations of 1:1 dilution for both solutions and the diluted sodium hydroxide solution (48%) was added firstly and after 24 hours a solution of hydrogen peroxide was added with twice the amount of sodium hydroxide. This work was based on the fact that the decomposition of hydrogen peroxide in the alkaline medium is accompanied by the release of oxygen, the occurrence of effervescence and the rise of impurities up to the surface of the solution in an exothermic reaction. This method which has been succeeded in removing sediments from the pipe of lengths up to 7 meters, can be considered a new addition in the field of chemical removal of sediments.
本研究旨在清除完全堵塞果汁澄清器中央分配管的固体沉淀物。由于澄清池内部管道的特殊条件,其直径狭窄且垂直位置,这些沉淀物无法通过破碎或加热等传统方法去除。因此,化学方法被用来解决这个问题。在此过程中采用了一种新的技术,即氢氧化钠和过氧化氢溶液的混合物。为了找到最合适的加入方式和最终所需的浓度,我们进行了多次实验和尝试,首先加入稀释后的氢氧化钠溶液(48%),24小时后加入两倍量的氢氧化钠溶液。这项工作是基于这样一个事实,即过氧化氢在碱性介质中的分解伴随着氧气的释放,气泡的发生以及在放热反应中杂质上升到溶液表面。该方法已成功地从长达7米的管道中去除沉积物,可被认为是化学去除沉积物领域的新成员。
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引用次数: 0
期刊
Egyptian Sugar Journal
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