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Production of Polyhydroxyalkanoate by Local Strain of Bacillus megaterium AUMC b 272 Utilizing Sugar Beet Wastewater and Molasses 大型芽孢杆菌AUMC b 272利用甜菜废水和糖蜜生产聚羟基烷酸酯
Pub Date : 2019-12-01 DOI: 10.21608/esugj.2019.219350
A. Zohri, A. K. Kamal El‐dean, Mohamed Abuo-Dobara, Mahmoud Ali, M. Bakr, Ramy Khashaba
Development of polyhydroxyalkanoates (PHAs) as a potential substitute material to some conventional plastics has drawn much attention due to their biodegradable and compatible properties. The potential applications of PHA in various industries and in the medical field are encouraging. Nevertheless, the production cost of PHA has been a major drawback. Consequently, scientific effort have been made to overcome the high cost of the substrates used in the bio-production. In this study, sugar beet industry wastewater without and with beet molasses was used as potential low cost substrate for production of the biopolymer PHA by a local bacterial strain. This strain was selected after screening of 30 bacterial isolates for PHA production and was identified according to 16S rRNA gene sequencing as Bacillus megaterium AUMC b 272. The maximum PHA formed by this strain reached to 4.053 g/L with a recovery yield equal to 41.79 % of the bacterial biomass using modified mineral salt medium (MSM) medium containing 6 % beet molasses as sole carbon source and 0.5 g/L ammonium oxalate as a nitrogen source. The initial pH of the medium was adjusted at 8.5. Cultures were incubated at 200 rpm and 37o C for 24 hrs. On the other hand, the bacterial strain produced negligible levels of PHA when grown on the same medium constituents under the same conditions with replaced distilled water and molasses by sugar beet wastewater. While the PHA concentration reached to 0.828 g/L with 46 Zohri A. A. et al (2019) Egyptian Sugar Journal, .Vol.13 ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ recovery yield 20.58 % of biomass in the same medium under the same conditions after replacement of distilled water by sugar beet wastewater. It is worthy to mention that the COD in the sugar beet wastewater medium at the end of fermentation period was removed by 69 %. Characterization of the obtained PHA was achieved using Fourier transform-infra-red spectroscopy (FT-IR) and gas chromatograph mass spectrometric (GCMS). Accumulation of considerable levels of PHA as well as high levels of COD removal from sugar beet wastewater strongly introduced this biotechnological process as valuable method for production of PHA as biodegradable biopolymer from sugar beet industry wastewater in presence of beet molasses as potential low cost substrates and, at the same time, for biological treatment of industry wastewater.
聚羟基烷酸酯(PHAs)由于具有生物可降解和相容性等特点,作为一些传统塑料的潜在替代材料而受到广泛关注。PHA在各行业和医疗领域的潜在应用前景令人鼓舞。然而,PHA的生产成本一直是一个主要缺点。因此,为了克服生物生产中使用的底物的高成本,科学努力已经进行了。在这项研究中,甜菜工业废水不含甜菜糖蜜和含有甜菜糖蜜作为潜在的低成本底物,通过当地菌株生产生物聚合物PHA。该菌株通过对30株产PHA菌株的筛选筛选出,经16S rRNA基因测序鉴定为芽孢杆菌巨型芽孢杆菌AUMC b 272。该菌株在以6%甜菜糖蜜为唯一碳源,0.5 g/L草酸铵为氮源的改性无机盐培养基(MSM)中,最大PHA生成量为4.053 g/L,回收率为细菌生物量的41.79%。将培养基的初始pH调整为8.5。在200 rpm和37℃下培养24小时。另一方面,在相同的培养基成分和相同的条件下,用甜菜废水代替蒸馏水和糖蜜生长时,菌株产生的PHA水平可以忽略不计。46 Zohri A. A. et al . (2019) egypt Sugar Journal, vol . 1。13ــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــــ收率达20.58%的生物量在同一介质在相同条件下甜菜废水后更换蒸馏水。值得一提的是,发酵末期甜菜废水培养基中的COD去除率达到69%。利用傅里叶变换红外光谱(FT-IR)和气相色谱质谱(GCMS)对合成的PHA进行了表征。从甜菜废水中积累相当水平的PHA以及高水平的COD去除强烈地引入了这种生物技术过程,作为从甜菜工业废水中生产PHA作为可生物降解的生物聚合物的有价值的方法,存在甜菜糖蜜作为潜在的低成本底物,同时用于工业废水的生物处理。
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引用次数: 4
Chemical Composition of Three Types of Low-grade Dates in Upper Egypt 上埃及三种低品位枣的化学成分
Pub Date : 2019-12-01 DOI: 10.21608/esugj.2019.219358
I. El-sayed, Mohamed Hussein, Adel Kamal El-Din
___________________________________________________________ Abstract The aim of the study was to determine the chemical composition of low-grade dates for three types of palm cultivated in Egypt (Phoenixdactylifera L.). in order To assess their sugars, crude protein, crude fat, ash and the moisture. The dates were rich in sugar (71.9–81.5% dry weight), while ash represented (2–2.31%). they contained low concentration of protein and very low concentration of fat (3.37–3.86% and 0.19 –0.26%, respectively),and moisture content (9-11%). Although the mineral contents varied widely, all varieties could be an important source of potassium. These results show that dates are nutritious and can play a major role in human nutrition and health. The high content of sugars found in low-grade dates makes significant chance of use it to produce of bio-alcohol.
___________________________________________________________摘要本研究旨在测定埃及种植的三种棕榈(Phoenixdactylifera L.)的低品位枣子的化学成分。以评估它们的糖、粗蛋白质、粗脂肪、灰分和水分。红枣干重的71.9 ~ 81.5%为高糖,干重的2 ~ 2.31%为灰分。蛋白质含量低,脂肪含量极低(分别为3.37 ~ 3.86%和0.19 ~ 0.26%),水分含量为9 ~ 11%。虽然矿物质含量差异很大,但所有品种都可能是钾的重要来源。这些结果表明,枣营养丰富,在人体营养和健康中发挥着重要作用。低档枣含糖量高,可用于生产生物酒精。
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引用次数: 0
Biotechnological β-glucan Production from Returned Baker´s Yeast and Yeast Remaining after Ethanol Fermentation 生物技术产β-葡聚糖的面包师酵母和乙醇发酵后的酵母残留物
Pub Date : 2019-12-01 DOI: 10.21608/esugj.2019.219349
A. Zohri, H. Moubasher, Hanfy Abdel-Hay, Mohamed Orban
The aim of this work was performed to develop a milder and cost effective extraction procedure for Beta-glucan (BG) from two types of S. cerevisiae, the returned Baker's yeast “RBY” and yeast remaining after ethanol fermentation “EFY” using different chemical extraction steps. The current results revealed that the carbohydrate percent in yeast biomass was considerably increased by the different extraction steps (from 37.64 to 92.41% and from 41.37 to 93.46% in case of RBY and EFY, respectively). On the other hand, the percent of protein in yeast biomass was decreased by the different extraction steps (from 41.91 to 1.28% and from 36.32 to 1.15% in the two cases, respectively). The extracted dry biomass in the two cases were analysed by Fourier Transform Infrared (FTIR) spectra. This method of β-glucan extraction steps has been shown to produce great yields of β-glucan with maintaining their purity and native structure.
本研究旨在通过不同的化学提取步骤,从两种酿酒酵母中提取β -葡聚糖(BG),即回收的贝克酵母(RBY)和乙醇发酵后剩余的酵母(EFY)。结果表明,不同提取步骤可显著提高酵母生物量中碳水化合物的比例(RBY和EFY分别从37.64提高到92.41%和41.37提高到93.46%)。另一方面,酵母生物量中蛋白质的比例随着提取步骤的不同而降低(分别从41.91降低到1.28%,从36.32降低到1.15%)。用傅里叶变换红外光谱对两种情况下提取的干生物质进行了分析。这种β-葡聚糖提取步骤已被证明可以在保持β-葡聚糖纯度和天然结构的情况下获得高产量的β-葡聚糖。
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引用次数: 5
Recent Achievements of Egyptian Sugarcane Breeding Program 埃及甘蔗育种计划的最新成果
Pub Date : 2019-06-01 DOI: 10.21608/esugj.2019.223909
Bazied Mohamed, Essam Amer, Mahmoud Ebid
Egyptian sugarcane breeding program depends upon natural flowering and artificial flowering. Natural flowering at El-Sabahia Agriculture Research Station (31.2 N latitude), Alexandria, Egypt is used to evaluate number of sugarcane germplasm for natural flowering ability and determine their flowering dates. The natural flowering season extendes from November, 1 to the end of June. Artificial flowering conducts at Giza Agriculture Research Station (30.01 N latitude), Giza, Egypt and used to estimate the number of inductive cycles for inducing the desirable parents from the available germplasms in the program to flower and synchronize their flowering dates to make planed crosses. Fuzz produced from planed crosses is used to establish the seedling stage, which follow by other selection stages to develop improving sugarcane variety. Increasing the size of the germplasm collection, promising clones at all selection stages and registration of Giza.3 and Giza.4 verities were the recent achievements of the breeding program. In addition to, resistant to smut disease Giza.3 and Giza.4 varieties have high cane and sugar yields. Besides, Giza.3 is an early mature variety and Giza.4 is a moderate mature variety.
埃及甘蔗育种计划依赖于自然开花和人工开花。利用埃及亚历山大El-Sabahia农业研究站(北纬31.2)的自然开花资料,评价甘蔗种质资源的自然开花能力,确定其开花日期。自然花期从11月1日持续到6月底。人工开花在埃及吉萨农业研究站(北纬30.01)进行,用于估计诱导周期的数量,以诱导该计划中可用的种质中理想的亲本开花并同步其开花日期以形成平面杂交。利用平面杂交产生的绒毛来确定苗期,然后通过其他选择阶段来培育甘蔗改良品种。增加种质资源的收集规模,在所有选择阶段都有前景的无性系和吉萨3号和吉萨4号品种的登记是该育种计划的最新成果。此外,抗黑穗病的Giza.3和Giza.4品种具有较高的甘蔗和糖产量。吉萨3号为早熟品种,吉萨4号为中成熟品种。
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引用次数: 1
Power quality improvement of sugar factories dc motor drive using hybrid filter 用混合滤波器改善糖厂直流电机传动的电能质量
Pub Date : 2019-06-01 DOI: 10.21608/esugj.2019.223913
Gaber M. Ahmed, Fareed. M. Mohamed, Mohamed Nosier
Power quality management is the main problem facing industry today. This problem is mainly due to the generation of electric network harmonics. The growing use of variable speed drives produces a large amount of harmonics in distribution systems because of the non-sinusoidal currents consumed by these drives. It is well known that for better quality of power, the voltage and current waveforms should be sinusoidal. But in actual practice it is somewhat distorted and this phenomenon is called “Harmonic Distortion”. Voltage harmonics are generally present in the utility power supply network. Even though electronic and non-linear devices are flexible, economical and energy efficient, they may degrade power quality by creating harmonic currents consuming excessive reactive power. Harmonic can be reduced using filters. Two types of filters have been utilized for harmonic distortions suppression namely passive and active filters. The present paper provides a method for designing a new hybrid (passive and active) power filters to reduce harmonic distortion and hence improve the power factor, reduce the cost and overcome all above problems. The simulation results certificate that the present hybrid filter (HAPF) causes perfect harmonics and reactive power compensation characteristics. In this case the total harmonic distortion (THD) meet standard values according to the IEEE 519/1992. The practical results of the studied dc motor drive system have been validated and compared with computer simulation MATLAB model results. The simulation results of this nonlinear studied system have been carried out with MATLAB Program 2010.
电能质量管理是当今工业面临的主要问题。这一问题主要是由于电网谐波的产生。由于变速驱动器消耗的非正弦电流,越来越多的变速驱动器在配电系统中产生了大量的谐波。众所周知,为了获得更好的电能质量,电压和电流波形应该是正弦波。但在实际应用中存在一定程度的失真,这种现象被称为“谐波失真”。电力系统中普遍存在电压谐波。尽管电子和非线性设备灵活、经济和节能,但它们可能会产生谐波电流,消耗过多的无功功率,从而降低电能质量。使用滤波器可以减少谐波。两种类型的滤波器被用于谐波失真抑制,即无源滤波器和有源滤波器。本文提出了一种新的混合型(无源和有源)电力滤波器的设计方法,以减少谐波失真,从而提高功率因数,降低成本,克服上述问题。仿真结果表明,该混合滤波器具有良好的谐波和无功补偿特性。在这种情况下,总谐波失真(THD)符合IEEE 519/1992的标准值。对所研究的直流电机驱动系统的实际结果进行了验证,并与计算机仿真的MATLAB模型结果进行了比较。利用MATLAB程序2010对所研究的非线性系统进行了仿真。
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引用次数: 0
Development and Testing of a Tractor-Drawn Sugarcane Billets Planter 牵引式甘蔗坯播种机的研制与试验
Pub Date : 2019-06-01 DOI: 10.21608/esugj.2019.223908
A. Gbabo, N. Baba, Akammaa Wada, O. Aturu
Research on mechanization of sugarcane production, however, is very low especially in the area of planting and currently there exists no known mechanical sugarcane planter in Nigeria. As a result, a mechanical planter was designed and fabricated. The main objective of the study is to develop and evaluate the performance of a tractordrawn sugarcane planter. The planter was designed, fabricated and tested in the Agricultural and Bioresources Department of The Federal University of Technology, Minna. Itconsists of a frame, hopper, cane seed metering device, delivery funnel, furrow opener, furrow covering/press wheel as well as drive wheels which transmits power to the metering device through chain drive arrangement. The performance tests of the fabricated machine were carried out using four levels of cane seed lengths (250mm, 300mm, 350mm and 400mm) at four levels of operational speeds (6km/h, 8km/h 10km/h and 12km/h) and with four levels of cane diameters (16mm, 20mm, 24mm and 28mm). Field capacity, seed rate, cane seed damage, miss index, multiple index and quality of feed index were measured. It was observed that the operational speed of the machine varies directly with machine capacity and miss index. While cane diameter varies directly with miss index, it had no significant effect on machine capacity. The machine was found to perform optimally at 10km/h operational speed, 300mm cane seed length and 28mm cane diameter. At this optimal speed level, the machine had field capacity of 0.53ha/h (4.24ha/day) and miss index of 20.8%. The developed machine could reduce drudgery involved in manual sugarcane planting and save about substantial amount of labour and operating time.
然而,对甘蔗生产机械化的研究非常少,特别是在种植领域,目前在尼日利亚没有已知的机械甘蔗播种机。为此,设计并制造了一种机械播种机。本研究的主要目的是开发和评价牵引式甘蔗播种机的性能。该播种机由米纳联邦科技大学农业和生物资源系设计、制造和测试。它由机架、料斗、蔗种计量装置、输送漏斗、开沟机、盖沟/压沟轮以及驱动轮组成,驱动轮通过链传动装置将动力传递给计量装置。在4种运行速度(6km/h、8km/h、10km/h和12km/h)和4种甘蔗直径(16mm、20mm、24mm和28mm)下,采用4种甘蔗种子长度(250mm、300mm、350mm和400mm)进行了性能测试。测定了田间容量、出苗率、蔗种损失率、脱粒指数、多重指数和饲料质量指数。观察到,机器的运行速度与机器容量和脱靶指数成正比。甘蔗直径与脱靶指数直接相关,但对机器产能影响不显著。实验发现,该机器在运行速度为10公里/小时,甘蔗种子长度为300毫米,甘蔗直径为28毫米时性能最佳。在此最佳转速水平下,现场处理量为0.53ha/h (4.24ha/day),脱靶指数为20.8%。该机器可减少人工种植甘蔗的繁重工作,节省大量的劳动力和操作时间。
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引用次数: 0
Opportunities of cooling crystallization for white sugar production 白糖生产中冷却结晶的机会
Pub Date : 2019-06-01 DOI: 10.21608/esugj.2019.223911
Y. Temerk, I. Abdullah, Osama Zien El-Abdein
Sugar cooling crystallization process was used for low sugar grade crystallization that carried out under certain conditions of higher supersaturation levels for further sugar crystallization after evaporating crystallization by decreasing sucrose solubility through temperature reduction. Recently there is a great interest with utilization of cooling and flash evaporation crystallization for white sugar production for energy cost saving in the sugar industry. This study is focusing on the utilization of an advanced sugar crystallization program to determine cooling crystallization parameters and conditions such as massecuite brix, crystal content and working temperature and pressure. A series of cooling crystallization experiments were conducted on a sugar crystallizer pilot at Al-khaleej sugar company “AKS” through 2017, 2018 seasons. Theoretical and practical simulation results are presented for sharing and discussions.
低糖级结晶采用糖冷却结晶工艺,在一定的较高过饱和度条件下,通过降温降低蔗糖的溶解度,在蒸发结晶后进行糖的进一步结晶。近年来,利用冷却和闪蒸结晶技术来生产白糖,以节省白糖工业的能源成本,受到了广泛的关注。本研究的重点是利用一种先进的糖结晶程序来确定冷却结晶参数和条件,如糖霜的白度、晶体含量和工作温度和压力。在Al-khaleej制糖公司“AKS”的糖结晶器试点上进行了一系列冷却结晶实验,持续到2017年和2018年。给出了理论和实际的仿真结果,供大家分享和讨论。
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引用次数: 0
Mordant yellow 12 dye removal from wastewater using low cost modified sugarcane bagasse as an adsorbent 以低成本改性甘蔗渣为吸附剂脱除废水中的媒染剂黄12染料
Pub Date : 2019-06-01 DOI: 10.21608/esugj.2019.223924
A. K. Kamal El‐dean, M. Ahmed, M. Mohamed, S. Hussain, E. Hashem
The adsorption behavior of mordant yellow 12 (4-amino phenyl azo salicylic sodium salt) (MY12) from aqueous solutions using low cost adsorbent citric acid or tartaric acid treated Sugarcane bagasse (SCB) was carried out. The experimental results have been investigated according to Langmuir and Freundlich isotherms. The effect of initial concentration of the dye, biosorbent dosage, temperature, contact time and pH were involved in the experiments. It was reported that the maximum removal percentage of MY12 was 95.2 % at pH 2.8 and contact time 40 minutes. The removal percentage decreases, as the concentration of the dye increases. Similar trend was found with the pH factor. The maximum biosorption capacities qe of MY12 by SCB was 8.2 mg g-1. It was concluded that; bagasse can be used as an efficient adsorbent material for the removal of organic dyes from aqueous solutions.
研究了柠檬酸和酒石酸处理的甘蔗渣(SCB)对介媒剂黄12(4-氨基苯基偶氮水杨酸钠盐)(MY12)的吸附行为。根据Langmuir等温线和Freundlich等温线对实验结果进行了研究。考察了染料初始浓度、生物吸附剂用量、温度、接触时间、pH值等因素的影响。在pH为2.8、接触时间为40 min的条件下,MY12的最大去除率为95.2%。随着染料浓度的增加,去除率降低。pH因子也有类似的变化趋势。SCB对MY12的最大吸附量为8.2 mg g-1。结论是;甘蔗渣可以作为一种有效的吸附材料,用于去除水溶液中的有机染料。
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引用次数: 0
Impact of bulk and nanoparticles zinc oxide foliar application on sugar beet yield and quality under different irrigation fertilization levels 不同灌溉施肥水平下散装和纳米氧化锌叶面施用对甜菜产量和品质的影响
Pub Date : 2019-06-01 DOI: 10.21608/esugj.2019.223912
Elmahdy Teama, Ali Othman, M. Farag, Mohamed E. Dahroug, Tawfik El-Kammash
Afield experiment was conducted at Abou El Ghar village, Kafr El Zayyat district, Gharbiya Governorate, Egypt during 20162017 and 2017-2018 seasons. The aim of this study to evaluate the influence of bulk and nanoparticles zinc oxide on growth and some biochemical characteristics of sugar beet (Beta vulgaris L.). The experiment was laid aut using strip-plot design with three replications. zinc oxide concentrations (control, 100, 200, 300 ppm) were arranged horizontally while nitrogen fertilizer (60, 80 and 100 Kg N/fed). Was allocated vertically.
野外试验于2016 - 2017年和2017-2018年在埃及Gharbiya省Kafr El Zayyat区Abou El Ghar村进行。本研究旨在探讨散装氧化锌和纳米氧化锌对甜菜生长及部分生化特性的影响。试验采用条带设计,重复3次。水平布置氧化锌浓度(对照、100、200、300 ppm),水平布置氮肥浓度(60、80、100 Kg N/次)。是垂直分配的。
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引用次数: 0
Five years’ performances of three key players in the restart journey of the Nigeria sugarcane and sugar industry 尼日利亚甘蔗和制糖业重启之旅中三个关键角色的五年表现
Pub Date : 2019-06-01 DOI: 10.21608/esugj.2019.223910
Akammaa Wada, A. Gbabo
Nigeria with suitable land and excellent edaphic environment for sugar cane production has not been lucky in the sugar business. Even with the potential to produce up to 30 million metric tons of sugar cane yielding 3.0 million metric tons of sugar from well over 800,000 ha of suitable land for sugar cane cultivation, this has not been attained. The country’s sugar industry totally collapsed in the late 20 century and was privatized between 1999 and 2002. Consequently, a deliberate sugar policy known as the backward integration (BIP) stemmed from the Nigeria Sugar Master Plan (NSMP) was conceived by the government through the National Sugar Development Council (NSDC) in 2012 to stimulate investment in the sugar industry. The implementation of the BIP was deliberately assigned to the three major privately owned sugar refineries in the country namely Dangote, BUA and Golden Sugar Company to drive its implementation in full. The Dangote group tagged its plan “Sugar for Nigeria Project” under the BIP plan to produce 1.5 to 2.0 MT/PA refined sugar from locally grown sugar cane in 10 years across various sites. The BUA Group invested in large scale sugar cane production in the country to deepen local sugar production through the acquisition of the Lafiagi Sugar Company Ltd in Kwara State in 2008. However, full transfer to BUA was not done till 2014. The Sunti Golden Sugar Company also initiated a five-year development plan in order to be fully integrated in the BIP plan and hit self-sufficiency in commercial sugar production by 2023. This paper highlights the growth status and challenges faced by the three pioneer sugar companies mid-way in their participation in the BIP to take Nigeria to the status of a sugar producing country by 2023.
尼日利亚拥有适合甘蔗生产的土地和优良的土壤环境,但在制糖业中并不幸运。即使有潜力在80多万公顷适合种植甘蔗的土地上生产多达3000万吨的甘蔗,产出300万吨的糖,但这一目标尚未实现。该国的制糖业在20世纪末完全崩溃,并在1999年至2002年间私有化。因此,政府在2012年通过国家糖业发展委员会(NSDC)构思了一项被称为“落后整合”(BIP)的糖政策,该政策源于尼日利亚糖业总体规划(NSMP),以刺激对糖业的投资。BIP的实施被故意分配给该国三家主要的私营糖厂,即Dangote, BUA和Golden sugar Company,以推动其全面实施。根据BIP计划,Dangote集团将其计划标记为“尼日利亚糖项目”,计划在10年内在不同地点从当地种植的甘蔗中生产1.5至2.0吨/年的精制糖。BUA集团于2008年通过收购Kwara州的Lafiagi糖业有限公司,在该国投资大规模甘蔗生产,以深化当地的糖生产。然而,直到2014年才完全转移到BUA。Sunti Golden糖业公司还启动了一项五年发展计划,以充分融入BIP计划,并在2023年之前实现商业糖生产的自给自足。本文重点介绍了三家先锋糖业公司在参与到2023年使尼日利亚成为糖生产国的BIP计划的过程中所面临的增长现状和挑战。
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引用次数: 0
期刊
Egyptian Sugar Journal
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