S. Mustafa, A. Estim, A. D. Tuzan, Chen Cheng Ann, L. Seng, S. R. M. Shaleh
This paper discusses the urgent need for human interventions in maximizing the promise of blue growth while ensuring sustainability in all its dimensions. It spares no efforts in highlighting the critical nexus between ocean conservation, climate change mitigation and the ecosystem services. The interpretation underscores the threat that unchecked deterioration of marine environment would present for health of the planet and its people. It is evident that the nature-based solutions provide the best options, but the significance of disruptive technologies and innovations cannot be underestimated. However, the decisions pertaining to devising and applying solutions should be informed by scientific reasoning and available evidence. Increasing attention given to blue economy shows the importance of exploring the sustainable solutions by shaping research that helps in identifying the tangible and integrated actions to fast track our progress towards implementing the Sustainable Development Goals.
{"title":"Nature-based and technology-based solutions for sustainable blue growth and climate change mitigation in marine biodiversity hotspots","authors":"S. Mustafa, A. Estim, A. D. Tuzan, Chen Cheng Ann, L. Seng, S. R. M. Shaleh","doi":"10.14799/EBMS302","DOIUrl":"https://doi.org/10.14799/EBMS302","url":null,"abstract":"This paper discusses the urgent need for human interventions in maximizing the promise of blue growth while ensuring sustainability in all its dimensions. It spares no efforts in highlighting the critical nexus between ocean conservation, climate change mitigation and the ecosystem services. The interpretation underscores the threat that unchecked deterioration of marine environment would present for health of the planet and its people. It is evident that the nature-based solutions provide the best options, but the significance of disruptive technologies and innovations cannot be underestimated. However, the decisions pertaining to devising and applying solutions should be informed by scientific reasoning and available evidence. Increasing attention given to blue economy shows the importance of exploring the sustainable solutions by shaping research that helps in identifying the tangible and integrated actions to fast track our progress towards implementing the Sustainable Development Goals.","PeriodicalId":11733,"journal":{"name":"Environmental biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87281425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Brzuzan, H. Marzec, F. Stefaniak, M. Woźny, M. Florczyk
Cyanobacteria constitute a rich source of biologically active and structurally diverse compounds. The pharmacological potential of these compounds resides among others in their ability to control the proliferation and growth of cancer cell lines and potent disease-causing microbial agents. Despite recent scientific advances, the way these compounds interact with the body’s molecular structure are still unclear and science still has to discover how the cyanobacterial metabolites interact with cell structures and how cells react to them. In this project, we will study yet unexamined cyanobacterial metabolites, especially the compounds which act as chemical ligands for microRNA (miRNA) -binding sites, making them promising regulators (inhibitors) of gene networks that are involved in various diseases. We will first develop a stable cell line that constitutively expresses a unique miRNA reporter system. Then, we will conduct a screen on chemical compounds discovered in Baltic cyanobacteria to identify small molecules with inhibitory activity and specificity to MIR92b-3p, which has a significant impact on liver cell behavior in humans. We assume that a successful MIR92b-3p inhibitor will bind to the precursors of MIR92b-3p miRNA, disabling the action of either of the two processing enzymes involved in the biogenesis of any miRNA in a cell (Drosha or Dicer), thus affecting the MIR92b function. The discoveries made with these inhibitory chemical molecules could provide insight into the role of the MIR92 pathway in liver diseases and cancer, and possibly, if promising results appear, they may facilitate a strategy for treating some human diseases in the future.
{"title":"Exploring Baltic Sea cyanobacteria for small-molecule inhibitors of microRNA function: a project description","authors":"P. Brzuzan, H. Marzec, F. Stefaniak, M. Woźny, M. Florczyk","doi":"10.14799/ebms301","DOIUrl":"https://doi.org/10.14799/ebms301","url":null,"abstract":"Cyanobacteria constitute a rich source of biologically active and structurally diverse compounds. The pharmacological potential of these compounds resides among others in their ability to control the proliferation and growth of cancer cell lines and potent disease-causing microbial agents. Despite recent scientific advances, the way these compounds interact with the body’s molecular structure are still unclear and science still has to discover how the cyanobacterial metabolites interact with cell structures and how cells react to them. In this project, we will study yet unexamined cyanobacterial metabolites, especially the compounds which act as chemical ligands for microRNA (miRNA) -binding sites, making them promising regulators (inhibitors) of gene networks that are involved in various diseases. We will first develop a stable cell line that constitutively expresses a unique miRNA reporter system. Then, we will conduct a screen on chemical compounds discovered in Baltic cyanobacteria to identify small molecules with inhibitory activity and specificity to MIR92b-3p, which has a significant impact on liver cell behavior in humans. We assume that a successful MIR92b-3p inhibitor will bind to the precursors of MIR92b-3p miRNA, disabling the action of either of the two processing enzymes involved in the biogenesis of any miRNA in a cell (Drosha or Dicer), thus affecting the MIR92b function. The discoveries made with these inhibitory chemical molecules could provide insight into the role of the MIR92 pathway in liver diseases and cancer, and possibly, if promising results appear, they may facilitate a strategy for treating some human diseases in the future.","PeriodicalId":11733,"journal":{"name":"Environmental biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73630374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
wetland containing cattail (Typha latifolia) was tested for treatment of synthetic PW. After 49 days, PW pH increased from 4.2 to 7.0, and electrical conductivity decreased from 22,100 to 3,300μS·cm-1. Typha shoots had bioconcentration factors for Pb ranging from 2.8 (Stage 1 of constructed wetland) to 8.0 (Stage 2). Transfer factors for Pb were 0.67 (Stage 1) and 1.37 (Stage 2). These results indicate that Typha may be effective for Pb removal from PWs. The present study may be of practical value to oil and gas production companies that plan to recycle or properly dispose of large quantities of oil and gas production wastewater. ABSTRACT
{"title":"Treatment of simulated oil and gas production wastewater using Typha latifolia in a pilot-scale constructed wetland","authors":"O. Alalade, J. Ferguson, J. Pichtel","doi":"10.14799/EBMS284","DOIUrl":"https://doi.org/10.14799/EBMS284","url":null,"abstract":"wetland containing cattail (Typha latifolia) was tested for treatment of synthetic PW. After 49 days, PW pH increased from 4.2 to 7.0, and electrical conductivity decreased from 22,100 to 3,300μS·cm-1. Typha shoots had bioconcentration factors for Pb ranging from 2.8 (Stage 1 of constructed wetland) to 8.0 (Stage 2). Transfer factors for Pb were 0.67 (Stage 1) and 1.37 (Stage 2). These results indicate that Typha may be effective for Pb removal from PWs. The present study may be of practical value to oil and gas production companies that plan to recycle or properly dispose of large quantities of oil and gas production wastewater. ABSTRACT","PeriodicalId":11733,"journal":{"name":"Environmental biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89459608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the current study attention has been focused on the evaluation of different methods of measurement of bacterial adhesion to the metal surfaces with different chemical composition. The direct methods, which included determination of the number of bacterial cells using fluorescence microscopy and Colony Forming Units (CFU) on agar medium, and indirect methods using Alamar Blue (AB) and MTT assays, were evaluated. The chemical compositions of the metal surfaces included: copper, iron, chromium and nickel. Interaction effects of assay and metal compound have been specifically demonstrated in this study. It was found that metal ions reacted with components of the indirect colorimetric tests used in this study (AB and MTT assay). Consequently, those tests gave positive false results. In contrast to the indirect methods, direct counting methods such as microscopy techniques and CFU counting, were successfully applied for evaluation of bacterial adhesion to the metal surfaces. However, considering the limits for the surfaces of the examined samples for microscopy, the determination of the CFU was found to be the best method for testing the adhesion to metal surfaces. The method, combined with the appropriate detachment procedures allowed for a precise determination of the number of bacteria on the entire surface of the evaluated metal samples.
在目前的研究中，人们关注的焦点是对不同化学成分的金属表面细菌粘附的不同测量方法的评价。对直接法(利用荧光显微镜和琼脂培养基上的菌落形成单位(CFU)测定细菌细胞数量)和间接法(利用Alamar Blue (AB)和MTT法)进行了评价。金属表面的化学成分包括:铜、铁、铬和镍。本研究特别证明了测定剂与金属化合物的相互作用。发现金属离子与本研究中使用的间接比色法(AB和MTT法)的组分发生反应。因此，这些测试给出了阳性的错误结果。与间接方法相比，显微镜技术和CFU计数等直接计数方法已成功应用于评估细菌对金属表面的粘附。然而，考虑到检测样品表面的显微镜限制，发现CFU的测定是测试金属表面附着力的最佳方法。该方法与适当的剥离程序相结合，可以精确测定被评估金属样品整个表面上的细菌数量。
{"title":"Measurement of bacterial adhesion to metal surfaces with different chemical composition - evaluation of different methods","authors":"A. Żywicka, K. Fijałkowski","doi":"10.14799/EBMS28","DOIUrl":"https://doi.org/10.14799/EBMS28","url":null,"abstract":"In the current study attention has been focused on the evaluation of different methods of measurement of bacterial adhesion to the metal surfaces with different chemical composition. The direct methods, which included determination of the number of bacterial cells using fluorescence microscopy and Colony Forming Units (CFU) on agar medium, and indirect methods using Alamar Blue (AB) and MTT assays, were evaluated. The chemical compositions of the metal surfaces included: copper, iron, chromium and nickel. Interaction effects of assay and metal compound have been specifically demonstrated in this study. It was found that metal ions reacted with components of the indirect colorimetric tests used in this study (AB and MTT assay). Consequently, those tests gave positive false results. In contrast to the indirect methods, direct counting methods such as microscopy techniques and CFU counting, were successfully applied for evaluation of bacterial adhesion to the metal surfaces. However, considering the limits for the surfaces of the examined samples for microscopy, the determination of the CFU was found to be the best method for testing the adhesion to metal surfaces. The method, combined with the appropriate detachment procedures allowed for a precise determination of the number of bacteria on the entire surface of the evaluated metal samples.","PeriodicalId":11733,"journal":{"name":"Environmental biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87554402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Kohlmann, P. Kersten, J. Gessner, D. Onara, E. Taflan, R. Suciu
{"title":"New microsatellite multiplex PCR sets for genetic studies of the sterlet sturgeon, Acipenser ruthenus","authors":"K. Kohlmann, P. Kersten, J. Gessner, D. Onara, E. Taflan, R. Suciu","doi":"10.14799/ebms285","DOIUrl":"https://doi.org/10.14799/ebms285","url":null,"abstract":"","PeriodicalId":11733,"journal":{"name":"Environmental biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82453137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Przemieniecki, T. Kurowski, K. Kotlarz, K. Krawczyk, M. Damszel, A. Karwowska
1Department of Entomology, Phytopathology and Molecular Diagnostics, Faculty of Environmental Management and Agriculture, University of Warmia and Mazury in Olsztyn, Prawocheƒskiego 17, 10‐720 Olsztyn, Poland 2 Virology and Bacteriology Department, Institute of Plant Protection National Research Institute, Władysława W ́gorka 20, 60-318 Poznaƒ, Poland 3Department of Plant Breeding and Seed Production, Faculty of Environmental Management and Agriculture, University of Warmia and Mazury in Olsztyn, Plac Łódzki 3, 10-724 Olsztyn, Poland
{"title":"Plant growth promoting properties of Serratia fonticola ART-8 and Pseudomonas putida ART-9 and their effect on the growth of spring wheat (Triticum aestivum L.)","authors":"S. Przemieniecki, T. Kurowski, K. Kotlarz, K. Krawczyk, M. Damszel, A. Karwowska","doi":"10.14799/EBMS263","DOIUrl":"https://doi.org/10.14799/EBMS263","url":null,"abstract":"1Department of Entomology, Phytopathology and Molecular Diagnostics, Faculty of Environmental Management and Agriculture, University of Warmia and Mazury in Olsztyn, Prawocheƒskiego 17, 10‐720 Olsztyn, Poland 2 Virology and Bacteriology Department, Institute of Plant Protection National Research Institute, Władysława W ́gorka 20, 60-318 Poznaƒ, Poland 3Department of Plant Breeding and Seed Production, Faculty of Environmental Management and Agriculture, University of Warmia and Mazury in Olsztyn, Plac Łódzki 3, 10-724 Olsztyn, Poland","PeriodicalId":11733,"journal":{"name":"Environmental biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78580310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
receptor, the androgen receptor, and the thyroid hormone receptor. Here, crosstalk is categorized as bidirectional or unidirectional, with the latter category further subdivided into ligand-dependent or –independent crosstalk. More research needs to be done to develop a clearer understanding of the involvement of receptor crosstalk in cell signaling that is induced by endocrine disruptors. This understanding will help to develop in vitro and in silico assays that can replace animal tests. ABSTRACT
{"title":"Endocrine disruptor actions through receptor crosstalk","authors":"R. Kiyama","doi":"10.14799/EBMS262","DOIUrl":"https://doi.org/10.14799/EBMS262","url":null,"abstract":"receptor, the androgen receptor, and the thyroid hormone receptor. Here, crosstalk is categorized as bidirectional or unidirectional, with the latter category further subdivided into ligand-dependent or –independent crosstalk. More research needs to be done to develop a clearer understanding of the involvement of receptor crosstalk in cell signaling that is induced by endocrine disruptors. This understanding will help to develop in vitro and in silico assays that can replace animal tests. ABSTRACT","PeriodicalId":11733,"journal":{"name":"Environmental biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89069060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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