A differential diet with royal jelly (RJ) during early larval development in honeybees shapes the phenotype, which is probably mediated by epigenetic regulation of gene expression. Evidence indicates that small molecules in RJ can modulate gene expression in mammalian cells, such as the fatty acid 10-hydroxy-2-decenoic acid (10-HDA), previously associated with the inhibition of histone deacetylase enzymes (HDACs). Therefore, we combined computational (molecular docking simulations) and experimental approaches for the screening of potential HDAC inhibitors (HDACi) among 32 RJ-derived fatty acids. Biochemical assays and gene expression analyses (Reverse Transcriptase - quantitative Polymerase Chain Reaction) were performed to evaluate the functional effects of the major RJ fatty acids, 10-HDA and 10-HDAA (10-hydroxy-decanoic acid), in two human cancer cell lines (HCT116 and MDA-MB-231). The molecular docking simulations indicate that these fatty acids might interact with class I HDACs, specifically with the catalytic domain of human HDAC2, likewise well-known HDAC inhibitors (HDACi) such as SAHA (suberoylanilide hydroxamic acid) and TSA (Trichostatin A). In addition, the combined treatment with 10-HDA and 10-HDAA inhibits the activity of human nuclear HDACs and leads to a slight increase in the expression of HDAC-coding genes in cancer cells. Our findings indicate that royal jelly fatty acids collectively contribute to HDAC inhibition and that 10-HDA and 10-HDAA are weak HDACi that facilitate the acetylation of lysine residues of chromatin, triggering an increase in gene expression levels in cancer cells.
{"title":"Exploring fatty acids from royal jelly as a source of histone deacetylase inhibitors: from the hive to applications in human well-being and health.","authors":"Fernanda Aparecida Dos Santos France,Debora Kazumi Maeda,Ana Beatriz Rodrigues,Mai Ono,Franciele Lopes Nogueira Marchetti,Marcos Martins Marchetti,Allana Cristina Faustino Martins,Roberto da Silva Gomes,Cláudia Aparecida Rainho","doi":"10.1080/15592294.2024.2400423","DOIUrl":"https://doi.org/10.1080/15592294.2024.2400423","url":null,"abstract":"A differential diet with royal jelly (RJ) during early larval development in honeybees shapes the phenotype, which is probably mediated by epigenetic regulation of gene expression. Evidence indicates that small molecules in RJ can modulate gene expression in mammalian cells, such as the fatty acid 10-hydroxy-2-decenoic acid (10-HDA), previously associated with the inhibition of histone deacetylase enzymes (HDACs). Therefore, we combined computational (molecular docking simulations) and experimental approaches for the screening of potential HDAC inhibitors (HDACi) among 32 RJ-derived fatty acids. Biochemical assays and gene expression analyses (Reverse Transcriptase - quantitative Polymerase Chain Reaction) were performed to evaluate the functional effects of the major RJ fatty acids, 10-HDA and 10-HDAA (10-hydroxy-decanoic acid), in two human cancer cell lines (HCT116 and MDA-MB-231). The molecular docking simulations indicate that these fatty acids might interact with class I HDACs, specifically with the catalytic domain of human HDAC2, likewise well-known HDAC inhibitors (HDACi) such as SAHA (suberoylanilide hydroxamic acid) and TSA (Trichostatin A). In addition, the combined treatment with 10-HDA and 10-HDAA inhibits the activity of human nuclear HDACs and leads to a slight increase in the expression of HDAC-coding genes in cancer cells. Our findings indicate that royal jelly fatty acids collectively contribute to HDAC inhibition and that 10-HDA and 10-HDAA are weak HDACi that facilitate the acetylation of lysine residues of chromatin, triggering an increase in gene expression levels in cancer cells.","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"2 1","pages":"2400423"},"PeriodicalIF":3.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-27DOI: 10.1080/15592294.2024.2380930
Andy Madrid, Joyce Koueik, Ligia A. Papale, Roy Chebel, Isabelle Renteria, Emily Cannon, Kirk J. Hogan, Reid S. Alisch, Bermans J. Iskandar
In mammals, the molecular mechanisms underlying transgenerational inheritance of phenotypic traits in serial generations of progeny after ancestral environmental exposures, without variation in DNA...
在哺乳动物中，表型性状在祖先暴露于环境后的连续几代后代中发生跨代遗传的分子机制，DNA...
{"title":"Folate-mediated transgenerational inheritance of sperm DNA methylation patterns correlate with spinal axon regeneration","authors":"Andy Madrid, Joyce Koueik, Ligia A. Papale, Roy Chebel, Isabelle Renteria, Emily Cannon, Kirk J. Hogan, Reid S. Alisch, Bermans J. Iskandar","doi":"10.1080/15592294.2024.2380930","DOIUrl":"https://doi.org/10.1080/15592294.2024.2380930","url":null,"abstract":"In mammals, the molecular mechanisms underlying transgenerational inheritance of phenotypic traits in serial generations of progeny after ancestral environmental exposures, without variation in DNA...","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"31 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Previous studies have indicated that histone methylations act as mediators in the relationship between oestrogen receptor (ER) and breast cancer prognosis, yet the mediating role has never been ass...
{"title":"H4K20me3, H3K4me2 and H3K9me2 mediate the effect of ER on prognosis in breast cancer","authors":"Cheng-Kun Xiao, Yuexiang Ren, Qianxin Chen, Yuanzhong Yang, Luying Tang, Lin Xu, Zefang Ren","doi":"10.1080/15592294.2024.2343593","DOIUrl":"https://doi.org/10.1080/15592294.2024.2343593","url":null,"abstract":"Previous studies have indicated that histone methylations act as mediators in the relationship between oestrogen receptor (ER) and breast cancer prognosis, yet the mediating role has never been ass...","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"9 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140636791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-14DOI: 10.1080/15592294.2024.2341578
Xin Li, Shan-Shan Xing, Sheng-Bo Meng, Zhong-Yi Hou, Lei Yu, Meng-Juan Chen, Dong-Dong Yuan, Hui-Fen Xu, Han-Fang Cai, Ming Li
Long non-coding RNAs (lncRNAs) have been shown to be involved in the regulation of skeletal muscle development through multiple mechanisms. The present study revealed that the lncRNA SOX6 AU (SRY-b...
{"title":"SOX6 AU controls myogenesis by cis-modulation of SOX6 in cattle","authors":"Xin Li, Shan-Shan Xing, Sheng-Bo Meng, Zhong-Yi Hou, Lei Yu, Meng-Juan Chen, Dong-Dong Yuan, Hui-Fen Xu, Han-Fang Cai, Ming Li","doi":"10.1080/15592294.2024.2341578","DOIUrl":"https://doi.org/10.1080/15592294.2024.2341578","url":null,"abstract":"Long non-coding RNAs (lncRNAs) have been shown to be involved in the regulation of skeletal muscle development through multiple mechanisms. The present study revealed that the lncRNA SOX6 AU (SRY-b...","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"77 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140602907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-09DOI: 10.1080/15592294.2024.2337085
Xueli Liu, Qina Chen, Xudong Yin, Xiao Wang, Jinshan Ran, Wei Yu, Bin Wang
The PhiC31 integration system allows for targeted and efficient transgene integration and expression by recognizing pseudo attP sites in mammalian cells and integrating the exogenous genes into the...
{"title":"Study on chromatin regulation patterns of expression vectors in the PhiC31 integration site","authors":"Xueli Liu, Qina Chen, Xudong Yin, Xiao Wang, Jinshan Ran, Wei Yu, Bin Wang","doi":"10.1080/15592294.2024.2337085","DOIUrl":"https://doi.org/10.1080/15592294.2024.2337085","url":null,"abstract":"The PhiC31 integration system allows for targeted and efficient transgene integration and expression by recognizing pseudo attP sites in mammalian cells and integrating the exogenous genes into the...","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"45 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-07DOI: 10.1080/15592294.2024.2337142
Nour El Osmani, Corinne Prévostel, Laurence Picque Lasorsa, Mohammad El Harakeh, Zeina Radwan, Hiba Mawlawi, Marwan El Sabban, Margret Shirinian, Zeina Dassouki
Deregulation of ten-eleven Translocation protein 1 (TET1) is commonly reported to induce imbalances in gene expression and subsequently to colorectal cancer development (CRC). On the other hand, vi...
{"title":"Vitamin C enhances co-localization of novel TET1 nuclear bodies with both Cajal and PML bodies in colorectal cancer cells","authors":"Nour El Osmani, Corinne Prévostel, Laurence Picque Lasorsa, Mohammad El Harakeh, Zeina Radwan, Hiba Mawlawi, Marwan El Sabban, Margret Shirinian, Zeina Dassouki","doi":"10.1080/15592294.2024.2337142","DOIUrl":"https://doi.org/10.1080/15592294.2024.2337142","url":null,"abstract":"Deregulation of ten-eleven Translocation protein 1 (TET1) is commonly reported to induce imbalances in gene expression and subsequently to colorectal cancer development (CRC). On the other hand, vi...","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"8 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-05DOI: 10.1080/15592294.2024.2333654
Stefanie Pilkay, Andie Riffer, Andrew Carroll
Many people experience traumatic or negative events, but few develop mental health issues as a result. This study investigated whether newborn DNA methylation (DNAm) previously associated with mate...
{"title":"Trauma context exerts intergenerational effects on child mental health via DNA methylation","authors":"Stefanie Pilkay, Andie Riffer, Andrew Carroll","doi":"10.1080/15592294.2024.2333654","DOIUrl":"https://doi.org/10.1080/15592294.2024.2333654","url":null,"abstract":"Many people experience traumatic or negative events, but few develop mental health issues as a result. This study investigated whether newborn DNA methylation (DNAm) previously associated with mate...","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"8 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-03DOI: 10.1080/15592294.2024.2333668
Jessica I. Lundin, Ulrike Peters, Yao Hu, Farah Ammous, Christy L. Avery, Emelia J. Benjamin, Joshua C. Bis, Jennifer A. Brody, Chris Carlson, Mary Cushman, Chris Gignoux, Xiuqing Guo, Jeff Haessler, Chris Haiman, Roby Joehanes, Silva Kasela, Eimear Kenny, Tuuli Lapalainien, Daniel Levy, Chunyu Liu, Yongmei Liu, Ruth J.F. Loos, Ake Lu, Tara Matise, Kari E. North, Sungshim L. Park, Scott M. Ratliff, Alex Reiner, Stephen S. Rich, Jerome I. Rotter, Jennifer A. Smith, Nona Sotoodehnia, Russell Tracy, David Van den Berg, Huichun Xu, Ting Ye, Wei Zhao, Laura M. Raffield, Charles Kooperberg, On Behalf of the PAGE Study
Systemic low-grade inflammation is a feature of chronic disease. C-reactive protein (CRP) is a common biomarker of inflammation and used as an indicator of disease risk; however, the role of inflam...
{"title":"Methylation patterns associated with C-reactive protein in racially and ethnically diverse populations","authors":"Jessica I. Lundin, Ulrike Peters, Yao Hu, Farah Ammous, Christy L. Avery, Emelia J. Benjamin, Joshua C. Bis, Jennifer A. Brody, Chris Carlson, Mary Cushman, Chris Gignoux, Xiuqing Guo, Jeff Haessler, Chris Haiman, Roby Joehanes, Silva Kasela, Eimear Kenny, Tuuli Lapalainien, Daniel Levy, Chunyu Liu, Yongmei Liu, Ruth J.F. Loos, Ake Lu, Tara Matise, Kari E. North, Sungshim L. Park, Scott M. Ratliff, Alex Reiner, Stephen S. Rich, Jerome I. Rotter, Jennifer A. Smith, Nona Sotoodehnia, Russell Tracy, David Van den Berg, Huichun Xu, Ting Ye, Wei Zhao, Laura M. Raffield, Charles Kooperberg, On Behalf of the PAGE Study","doi":"10.1080/15592294.2024.2333668","DOIUrl":"https://doi.org/10.1080/15592294.2024.2333668","url":null,"abstract":"Systemic low-grade inflammation is a feature of chronic disease. C-reactive protein (CRP) is a common biomarker of inflammation and used as an indicator of disease risk; however, the role of inflam...","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"8 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1080/15592294.2024.2333660
Wei Zhang, Juan I. Young, Lissette Gomez, Michael A. Schmidt, David Lukacsovich, Achintya Varma, X. Steven Chen, Brian Kunkle, Eden R. Martin, Lily Wang
DNA methylation (DNAm) plays a crucial role in a number of complex diseases. However, the reliability of DNAm levels measured using Illumina arrays varies across different probes. Previous research...
DNA 甲基化（DNAm）在多种复杂疾病中起着至关重要的作用。然而，使用 Illumina 阵列测量的 DNAm 水平的可靠性因探针的不同而不同。以前的研究...
{"title":"Critical evaluation of the reliability of DNA methylation probes on the Illumina MethylationEPIC v1.0 BeadChip microarrays","authors":"Wei Zhang, Juan I. Young, Lissette Gomez, Michael A. Schmidt, David Lukacsovich, Achintya Varma, X. Steven Chen, Brian Kunkle, Eden R. Martin, Lily Wang","doi":"10.1080/15592294.2024.2333660","DOIUrl":"https://doi.org/10.1080/15592294.2024.2333660","url":null,"abstract":"DNA methylation (DNAm) plays a crucial role in a number of complex diseases. However, the reliability of DNAm levels measured using Illumina arrays varies across different probes. Previous research...","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"2 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.1080/15592294.2024.2337087
Jia-Xue Sun, Yongli Yao, Wen-Xuan Li, Xin Su, Haoyu Yang, Zhouping Lu, Chenfei Liu, Xiang-Hong Xu, Liping Jin
Decidual macrophages are the second-largest immune cell group at the maternal-foetal interface. They participate in apoptotic cell removal, and protect the foetus from microorganisms or pathogens. ...
{"title":"Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage","authors":"Jia-Xue Sun, Yongli Yao, Wen-Xuan Li, Xin Su, Haoyu Yang, Zhouping Lu, Chenfei Liu, Xiang-Hong Xu, Liping Jin","doi":"10.1080/15592294.2024.2337087","DOIUrl":"https://doi.org/10.1080/15592294.2024.2337087","url":null,"abstract":"Decidual macrophages are the second-largest immune cell group at the maternal-foetal interface. They participate in apoptotic cell removal, and protect the foetus from microorganisms or pathogens. ...","PeriodicalId":11767,"journal":{"name":"Epigenetics","volume":"52 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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