Yasuhiro Tanizawa, J. Mashima, T. Fujisawa, Kyungbum Lee, Yasukazu Nakamura, K. Shimizu, K. Kadota
{"title":"Methods for analyzing next-generation sequencing data IX.Genome annotation, visualization, and registration to DDBJ","authors":"Yasuhiro Tanizawa, J. Mashima, T. Fujisawa, Kyungbum Lee, Yasukazu Nakamura, K. Shimizu, K. Kadota","doi":"10.4109/JSLAB.28.3","DOIUrl":"https://doi.org/10.4109/JSLAB.28.3","url":null,"abstract":"","PeriodicalId":117947,"journal":{"name":"Japanese Journal of Lactic Acid Bacteria","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121378688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rika Hiraoka, Isao Uda, S. Nakano, N. Shimomura, T. Aimi
Using gastric juice tolerant Lactobacillus plantarum FSCM2-12, NBRC 109604 and NBRC 101975, and gastric juice sensitive Lactobacillus brevis FSCT-2, the relationship between the gastric acid tolerance and the morphologic characteristics were examined. Survival ratio of L. plantarum FSCM2-12 cultured in the medium supplemented with glucose, fructose, or sucrose as a carbon source, was more than 70% after treatment with the artificial gastric juice. However, the survival ratio of the strain cultured in the medium supplemented with glycerol or no carbon source, was drastically decreased. L. plantarum FSCM2-12 did not form the biofilm, but clear capsule was observed under the microscope after Hiss staining and by transmission electron microscopic observation. Furthermore, thickness of the capsule showed correlation with gastric juice tolerance. The capsules were thick and the survival ratio after treatment with artificial gastric juice was more than 70% when the strain was cultured in the medium supplemented with glucose, fructose, and sucrose as a carbon sources. Similar phenomena were observed in L. plantarum NBRC 109604 and NBRC 101975, therefore, the high gastric acid tolerance might be provided by
以胃液耐受型植物乳杆菌FSCM2-12、NBRC 109604和NBRC 101975以及胃液敏感型短乳杆菌FSCT-2为材料，研究胃酸耐受与形态特征的关系。在以葡萄糖、果糖或蔗糖为碳源的培养基中培养的植物乳杆菌FSCM2-12，经人工胃液处理后的成活率可达70%以上。然而，在添加甘油或不添加碳源的培养基中培养的菌株存活率急剧下降。L. plantarum FSCM2-12未形成生物膜，但经Hiss染色和透射电镜观察，显微镜下可观察到透明的荚膜。此外，胶囊的厚度与胃液耐受性相关。在以葡萄糖、果糖、蔗糖为碳源的培养基中培养，荚膜较厚，经人工胃液处理后的存活率可达70%以上。在植物乳杆菌NBRC 109604和NBRC 101975中也观察到类似的现象，因此，高胃酸耐受性可能是由
{"title":"Relationship between gastric acid tolerance and capsule formation in plant origin lactic acid bacteria, Lactobacillus plantarum FSCM2-12","authors":"Rika Hiraoka, Isao Uda, S. Nakano, N. Shimomura, T. Aimi","doi":"10.4109/JSLAB.27.196","DOIUrl":"https://doi.org/10.4109/JSLAB.27.196","url":null,"abstract":"Using gastric juice tolerant Lactobacillus plantarum FSCM2-12, NBRC 109604 and NBRC 101975, and gastric juice sensitive Lactobacillus brevis FSCT-2, the relationship between the gastric acid tolerance and the morphologic characteristics were examined. Survival ratio of L. plantarum FSCM2-12 cultured in the medium supplemented with glucose, fructose, or sucrose as a carbon source, was more than 70% after treatment with the artificial gastric juice. However, the survival ratio of the strain cultured in the medium supplemented with glycerol or no carbon source, was drastically decreased. L. plantarum FSCM2-12 did not form the biofilm, but clear capsule was observed under the microscope after Hiss staining and by transmission electron microscopic observation. Furthermore, thickness of the capsule showed correlation with gastric juice tolerance. The capsules were thick and the survival ratio after treatment with artificial gastric juice was more than 70% when the strain was cultured in the medium supplemented with glucose, fructose, and sucrose as a carbon sources. Similar phenomena were observed in L. plantarum NBRC 109604 and NBRC 101975, therefore, the high gastric acid tolerance might be provided by","PeriodicalId":117947,"journal":{"name":"Japanese Journal of Lactic Acid Bacteria","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126550556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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