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Japanese Journal of Lactic Acid Bacteria最新文献

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Response is differed among the regions of the small intestine by lactic acid bacteria administration 反应是不同的区域之间的小肠乳酸菌管理
Pub Date : 2019-03-14 DOI: 10.4109/jslab.30.32
T. Tsukahara
To determine the functionality and safety of lactic acid bacteria (LAB), the study using model animals was performed frequently. However, with ambiguous study settings, there is a possibility of overlooking the efficacy of LAB. In this review, I mentioned important information about the small intestine that was one of the main functional organs by LAB administration. Small intestine was not responded universally by LAB administration, whereas it was responded region-specific. It is well known that LAB administration induced villous elongation in the weaning piglets, however this elongation was only detected at middle and ileo-cecal junction of the small intestine. Peyer’s patch (PP) is a main gut immune site in the small intestine. When mice with LAB administration were sacrificed and collected the PP around the duodenum (jPP) and PP around the ileo-cecal junction (iPP), cytokine expressions were differed between the jPP and iPP. Furthermore, cytokine expression was differed among the time after administration of LAB
为了确定乳酸菌(LAB)的功能和安全性,经常使用模型动物进行研究。然而,由于研究设置不明确,有可能忽视LAB的功效。在这篇综述中,我提到了小肠作为主要功能器官之一的重要信息。小肠对乳酸菌没有普遍的反应,而有区域特异性反应。众所周知,乳酸菌可诱导断奶仔猪的绒毛伸长,但这种伸长仅在小肠中部和回肠-盲肠交界处可见。Peyer’s patch (PP)是小肠中一个主要的肠道免疫部位。给药后处死小鼠,收集十二指肠周围的PP (jPP)和回肠-盲肠交界处的PP (iPP),观察jPP和iPP细胞因子的表达差异。此外,给药后不同时间的细胞因子表达也不同
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引用次数: 0
Functional analysis of biosynthetic genes for bacteriocins 细菌素生物合成基因的功能分析
Pub Date : 2019-03-14 DOI: 10.4109/jslab.30.18
T. Zendo, Shun Iwatani, K. Sonomoto
Among bacteriocins, ribosomally synthesized bacterial antimicrobial peptides, those produced by lactic acid bacteria, in particular, are expected to be used as safe antimicrobial agents for food preservation. Novel bacteriocins with excellent properties have been extensively explored, and functional analysis of the biosynthetic genes has been conducted to elucidate its structure, biosynthesis mechanism and action mechanism. The biosynthetic proteins are encoded on the vicinity of the bacteriocin precursor gene, and the biosynthetic gene cluster includes the genes encoding proteins responsible for self-immunity against their own bacteriocins, secretion of bacteriocins, posttranslational modification and production regulation. The functions of these biosynthetic genes are expected to be applied to secretory production of peptides and introduction of modified structures to various peptides. In this review, we will introduce our novel bacteriocins produced by lactic acid bacteria, focusing on determination of biosynthetic genes and their
在细菌素中,核糖体合成的细菌抗菌肽,特别是乳酸菌产生的细菌抗菌肽,有望作为安全的食品防腐抗菌剂使用。具有优异性能的新型细菌素得到了广泛的探索,并对其生物合成基因进行了功能分析,以阐明其结构、生物合成机制和作用机制。生物合成蛋白编码在细菌素前体基因附近,生物合成基因簇包括编码对自身细菌素的自身免疫、细菌素分泌、翻译后修饰和生产调控的蛋白质的基因。这些生物合成基因的功能有望应用于肽的分泌生产和各种肽的修饰结构的引入。本文将介绍由乳酸菌产生的新型细菌素,重点介绍其生物合成基因的测定及其作用机制
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引用次数: 0
ABCs for analysis of gut microbiota 用于分析肠道微生物群的abc
Pub Date : 2019-03-14 DOI: 10.4109/jslab.30.27
R. Inoue
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引用次数: 0
Methodology of the isolation of lactic acid bacteria strains 乳酸菌菌株分离的方法学
Pub Date : 2019-03-14 DOI: 10.4109/jslab.30.3
N. Tanaka
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引用次数: 1
The directions and methods of genetic engineering and cultivation of lactic acid bacteria for the beginners 乳酸菌基因工程与培养的方向与方法供初学者参考
Pub Date : 2019-03-14 DOI: 10.4109/jslab.30.8
K. Okano
Genetic engineering of lactic acid bacteria is a difficult task for the beginners due to large difference in strains of interest and poor access for genetic tools. In this review, based on the author’ s experiences, experimental directions and methods for selecting the host strain and vectors; transformation of lactic acid bacteria; optimization of expression system; gene disruption/insertion; cultivation of recombinants
乳酸菌的基因工程对初学者来说是一项艰巨的任务,因为感兴趣的菌株差异很大,遗传工具的获取也很困难。本文根据作者的经验,对宿主菌株和载体的选择、实验方向和方法进行了综述;乳酸菌转化;表达体系优化;基因中断/插入;重组体的培养
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引用次数: 0
Alcohol fermentation of milk by multiple fermentation 牛奶经多次发酵后的酒精发酵
Pub Date : 2018-11-06 DOI: 10.4109/jslab.29.145
Yoshihisa Wakita, H. Kanda, Koji Takazumi, Y. Tsuchiya
Saccharomyces cerevisiae is a representative yeast used for alcohol production. Production of alcohol from milk using S. cerevisiae is not easy, as this species is unable to hydrolyse lactose. Lactobacillus delbrueckii subsp. bulgaricus (LDB) is able to hydrolyse lactose into galactose and glucose, and is widely used in yogurt production. In this study, production of alcohol from milk was attempted by multiple fermentation using S. cerevisiae and LDB. Firstly, strain LDB48A-12, released from catabolite repression, was obtained from LDB48P by N-methyl-N ’-nitro-N-nitrosoguanidine mutagenesis. LDB48A-12 showed high ability of lactose degradation. Surprisingly, growth speed and lactate production of LDB48A-12 were found to be lower than those of LDB48P. Fermentation of 10% skim milk solution using LDB48A-12 and S. cerevisiae SBC3207 for 4 days at 30℃ or 37℃ produced approximately 1~1.5% of alcohol, which was 1.5 times that produced using LDB48P. In addition, decrease in pH was inhibited. These results were thought to demonstrate novel alcoholic milk fermentation. Comparison of genome sequences revealed mutations in RpoA and DnaA in LDB48A-12. These genes are related to catabolite repression and replication of genome DNA, respectively, suggesting that combined mutation of these genes leads to the preferable phenotype for alcoholic milk fermentation.
酿酒酵母是一种用于酒精生产的代表性酵母。利用酿酒酵母从牛奶中生产酒精并不容易,因为这种细菌不能水解乳糖。德尔布鲁氏乳杆菌亚种保加利亚菌(LDB)能够将乳糖水解成半乳糖和葡萄糖,广泛用于酸奶生产。在本研究中,利用酿酒酵母和LDB进行多次发酵,以牛奶为原料生产酒精。首先,通过n -甲基-n ' -硝基-n -亚硝基胍诱变LDB48P获得了分解代谢抑制释放的菌株LDB48A-12。LDB48A-12具有较高的乳糖降解能力。令人惊讶的是,LDB48A-12的生长速度和乳酸产量低于LDB48P。用LDB48A-12和酿酒酵母SBC3207在30℃或37℃下发酵4天,10%脱脂乳溶液的酒精产量约为1~1.5%,是用LDB48P发酵的1.5倍。此外,pH值的下降也被抑制。这些结果被认为证明了新的酒精牛奶发酵。基因组序列比较发现LDB48A-12中存在RpoA和DnaA突变。这些基因分别与分解代谢物抑制和基因组DNA复制有关,表明这些基因的组合突变导致了酒精牛奶发酵的有利表型。
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引用次数: 0
Effects of Lactobacillus paracasei K71 on gut microbiota composition and lipid metabolism in ob/ob mice. 副干酪乳杆菌K71对ob/ob小鼠肠道菌群组成和脂质代谢的影响
Pub Date : 2018-11-06 DOI: 10.4109/jslab.29.152
Yuh Shiwa, Satomi Ito, Y. Matsumoto, Tsukasa Suzuki, T. Ishige, T. Kumagai, N. Fujita, Yuji Yamamoto, N. Tanaka
We investigated the effects of heat-killed Lactobacillus paracasei K71 (K71)on lipid metabolism and gut microbiota in an ob/ob mouse model of obesity. Wild-type and ob/ob (ob-AIN group) mice were fed an AIN-93G diet or an AIN-93G diet containing K71 (ob-K71 group) for 90 d. Serum lipids, hepatic gene expression, and gut microbial populations were evaluated. K71 intake had no significant effect on body weight or lipid metabolism in the liver, while serum non-esterified fatty acids (NEFA) were lower in the ob-K71 group than in the ob-AIN group. In addition, the expression of serine palmitoyl transferase (SPT)-1, a key enzyme in the formation of ceramides associated with insulin resistance, was also lower in the livers of the ob-K71 group than in those of the ob-AIN group. Sequencing of the 16S ribosomal RNA gene revealed that K71 intake suppressed the changes in gut microbiota related to type 2 diabetes and nonalcoholic steatohepatitis. Our results suggest that K71 ingestion alters gut microbiota composition and improves insulin resistance via the ceramide
我们研究了热灭活副干酪乳杆菌K71 (K71)对肥胖小鼠脂质代谢和肠道微生物群的影响。野生型和ob/ob型(ob- ain组)小鼠分别饲喂AIN-93G饲粮和AIN-93G饲粮(ob-K71组)90 d,观察血清脂质、肝脏基因表达和肠道微生物数量。摄入K71对体重和肝脏脂质代谢无显著影响,而ob-K71组血清非酯化脂肪酸(NEFA)低于ob-AIN组。此外,ob-K71组肝脏中丝氨酸棕榈酰转移酶(SPT)-1的表达也低于ob-AIN组,SPT -1是与胰岛素抵抗相关的神经酰胺形成的关键酶。16S核糖体RNA基因测序显示,摄入K71抑制了与2型糖尿病和非酒精性脂肪性肝炎相关的肠道微生物群的变化。我们的研究结果表明,摄入K71可以改变肠道菌群组成,并通过神经酰胺改善胰岛素抵抗
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引用次数: 0
Methods for analyzing next-generation sequencing data XII.Galaxy - Sharing histories and workflows 新一代测序数据分析方法12。星系-共享历史和工作流程
Pub Date : 2018-07-02 DOI: 10.4109/jslab.29.79
Tomoko Terada, Tazro Ohta, K. Shimizu, K. Kadota
Galaxy is an integrative data analysis environment run on the web browser which users can use without using Linux command line. The previous article showed an introduction to the Galaxy system and the basic usage of the public Galaxy server “Galaxy Main.” In this article, using the last article’s results, we present the features to share the analysis results (history) with the other users, or ones to apply the analysis procedures (workflow) to the other data. We also show the useful Galaxy’s features such as copying data across histories, or the direct data import from remote servers. We found a compatibility issue on Internet Explorer with the Galaxy system. Thus we recommend using Google Chrome or Firefox to try the procedures we show in this article. Supplementary materials are available online at: http://www.iu.a.u-tokyo.ac.jp/~kadota/r_seq.html#about_book_JSLAB.
Galaxy是一个运行在web浏览器上的综合数据分析环境,用户无需使用Linux命令行即可使用。前一篇文章介绍了Galaxy系统和公共Galaxy服务器“Galaxy Main”的基本用法。在本文中,使用上一篇文章的结果,我们展示了与其他用户共享分析结果(历史)的特性,或者将分析过程(工作流)应用于其他数据的特性。我们还展示了有用的Galaxy功能,例如跨历史复制数据,或从远程服务器直接导入数据。我们发现ie浏览器与Galaxy系统存在兼容性问题。因此,我们建议使用Google Chrome或Firefox来尝试本文中展示的过程。补充资料可在网上查阅:http://www.iu.a.u-tokyo.ac.jp/~kadota/r_seq.html#about_book_JSLAB。
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引用次数: 0
Potential probiotic lactic acid bacterium Lactococcus lactis H61 contributing to high quality of life 潜在的益生菌乳酸菌乳酸乳球菌H61有助于提高生活质量
Pub Date : 2018-07-02 DOI: 10.4109/jslab.29.69
H. Kimoto-Nira
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引用次数: 0
乳酸菌株の個性を追いかけ 45 年 追求乳酸菌株个性45年
Pub Date : 2018-03-10 DOI: 10.4109/JSLAB.29.3
Sanae Okada
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引用次数: 0
期刊
Japanese Journal of Lactic Acid Bacteria
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