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First report of field sampling and identification of crown-of-thorns starfish larvae 刺冠海星幼虫野外取样鉴定首次报道
Pub Date : 1900-01-01 DOI: 10.3755/GALAXEA.17.15
N. Yasuda, K. Kajiwara, S. Nagai, Kota Ikehara, K. Nadaoka
To date, field sampling and identification of the larvae of the coral-eating starfish Acanthaster planci have not been successfully performed (Birkeland 1990). We collected water samples (500 L) from 5 different depths (surface, 3 m, 7 m, 11 m, 15 m, and near the bottom) to find A. planci larvae on June 29, 2005, during the spawning period (Yasuda et al. 2010). The samples were obtained from Yabiji Reef, off Miyako Island, Okinawa, Japan (25°1′13.85′′N, 125°14′49.20′′E, Fig. 1A), where a population outbreak of A. planci was observed (Fig. 1B). We used a stereoscopic microscope to find and isolate 26 possible starfish larvae (gastrula and bipinnaria stages; Fig. 1C and D, respectively) from the samples, which were preserved in ethanol (>70%). We extracted DNA using the DNeasy blood & tissue kit (Qiagen), and directly sequenced partial mitochondrial DNA fragments amplified by using a previously published COI primer pair (Vogler et al. 2008) and a putative control region primer pair (Timmers et al. 2011). The latter primer pair can discriminate A. planci from some common sea stars such as Protoreaster nodosus, Linckia laevigata, and Culcita novaeguineae. The following PCR conditions were used: denaturing step at 94°C for 3 min, followed by 35 cycles of 94°C for 20 s, 60°C for 30 s, and 72°C for 30 s with a final extension step at 72°C for 5 min. The PCR cocktail contained 3.84 μL of ddH2O, 5 μL of KAPATaq Extra HotStart ReadyMix (Kapa Biosystems), 1 μL of template DNA, and 0.07 μL of 50 mM primers. Only 10 larvae were successfully analyzed, partly due to degradation of DNA, but all were identified as A. planci by BLAST search. Nine larvae at the gastrula stage were found in either the surface (2), 7 m (5), or near bottom (2) samples, while 1 larva at the bipinnaria stage was found in the surface sample. Salinity-Temperature-Depth (STD) data showed that the water temperature was 27.7-27.9°C and chlorophyll a concentration was 0.3-0.4 μg/L at the sampling points from where we identified larvae of A. planci. Since the minimum threshold density of chlorophyll a for successful development is 0.4 μg/L (Fabricius et al. 2010), the larvae might have had access to limited food during the study period. First report of field sampling and identification of crown-of-thorns starfish larvae
迄今为止,尚未成功地对食珊瑚海星棘星(Acanthaster planci)的幼虫进行实地取样和鉴定(Birkeland 1990)。2005年6月29日,我们从5个不同的深度(表层、3米、7米、11米、15米和接近底部)采集了500 L的水样,在产卵期发现了planci的幼虫(Yasuda et al. 2010)。样本来自日本冲绳县宫古岛外的Yabiji Reef(25°1′13.85”N, 125°14′49.20”E,图1A),在那里观察到planci种群爆发(图1B)。利用立体显微镜发现并分离了26只可能的海星幼虫(原肠期和双足期;分别为图1C和D),样品保存在乙醇(>70%)中。我们使用DNeasy血液和组织试剂盒(Qiagen)提取DNA,并使用先前发表的COI引物对(Vogler et al. 2008)和假定的控制区引物对(Timmers et al. 2011)直接对部分线粒体DNA片段进行测序。后一对引物可以区分planci和一些常见的海星,如Protoreaster nodosus、Linckia laevigata和Culcita novaeguineae。采用以下PCR条件:94°C变性步骤3 min, 94°C变性步骤20 s, 60°C变性步骤30 s, 72°C变性步骤30 s,最后延长步骤72°C扩增步骤5 min,共35次循环。PCR混合物中含有3.84 μL ddH2O, 5 μL KAPATaq Extra HotStart ReadyMix (Kapa Biosystems), 1 μL模板DNA和0.07 μL 50 mM引物。只有10只幼虫被成功分析,部分原因是DNA的降解,但所有幼虫都被BLAST搜索确定为planci。表面(2)、7 m(5)和近底部(2)样品中均发现原肠期幼虫9只,表面样品中发现双翅虫期幼虫1只。盐度-温度-深度(STD)数据显示,该取样点的水温为27.7 ~ 27.9°C,叶绿素a浓度为0.3 ~ 0.4 μg/L。由于成功发育的叶绿素a最低阈值密度为0.4 μg/L (Fabricius et al. 2010),因此在研究期间,幼虫可能只能获得有限的食物。刺冠海星幼虫野外取样鉴定首次报道
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引用次数: 5
Behavior of dissolved cadmium in surface water of Urasoko Bay, Ishigaki Island, Japan 日本石垣岛浦古古湾地表水中溶解镉的行为
Pub Date : 1900-01-01 DOI: 10.3755/GALAXEA.10.77
K. Abe
The concentrations of dissolved cadmium (Cd) were monitored in the surface water of Urasoko Bay and the mouth of the stream that runs into the bay. Urasoko Bay is located on the northern coast of Ishigaki Island, Okinawa, which is surrounded by a fringing reef. Water samplings were carried out from August 2006 to August 2007, and adding to these samplings, freshwater from the upper stream and brackish water that exudates at the beach site were collected from April to June 2007. The concentration of dissolved Cd showed no tendency to decrease from the upper stream to the bay site. The results of Cd behaved non-conservatively in the Cd-salinity plot, which was attributed to daily variations in the Cd concentrations of upper stream water and the irregular input of Cd possibly adsorbed particles into the stream and beach water (the mixture of groundwater and seawater before exudation on the beach).
监测了浦asoko湾地表水和流入浦asoko湾的河流口中溶解镉(Cd)的浓度。浦asoko湾位于冲绳石垣岛北部海岸,周围环绕着珊瑚礁。在2006年8月至2007年8月期间进行了水质采样,并在2007年4月至6月期间收集了上游的淡水和在海滩地点渗出的微咸水。从上游到海湾,溶解Cd的浓度没有下降的趋势。Cd-盐度图中Cd的结果表现出非保守性,这是由于上游水体Cd浓度的每日变化以及Cd可能被吸附的颗粒不规则地输入到溪流和海滩水(在海滩上渗出之前的地下水和海水的混合物)。
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引用次数: 1
Filling an empty role: first report of cleaning by pygmy angelfishes (Centropyge, Pomacanthidae) 填补空白的作用:小天使鱼清洁的第一次报道(圆棘鱼科)
Pub Date : 1900-01-01 DOI: 10.3755/galaxea.22.1_31
P. Narvaez, R. Morais
Cleaner fishes remove ectoparasites, mucus and dead tissues from other ‘client’ organisms. These mutu­ alistic interactions provide benefits for the ‘clients’ and, on a larger scale, maintain healthy reef ecosystems. Here, we report two species of angelfishes, Centropyge bicolor and C. tibicen, acting as cleaners of the blue tang Paracanthurus hepatus in an aquarium. This observation is the first time that pygmy angelfishes are recorded cleaning in any en vironment. This novel cleaning ob­ servation raises ques tions on the ecosystem role of cleaner fishes and which biological traits facilitate cleaning.
清洁鱼从其他“客户”生物体中清除体外寄生虫、粘液和死组织。这些相互作用为“客户”提供了好处,在更大的范围内,维持了健康的珊瑚礁生态系统。在这里,我们报道了两种天使鱼,Centropyge bicolor和C. tibicen,在水族馆中充当蓝塘肝旁棘鱼的清洁工。这是首次记录到侏儒天使鱼在任何环境中进行清洁。这种新颖的清洁观察提出了关于清洁鱼的生态系统作用和哪些生物特性促进清洁的问题。
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引用次数: 2
Space competition between coral and algae — effect of two functional groups of algae on juvenile Acropora corals 珊瑚与藻类的空间竞争——两种藻类官能团对鹿角珊瑚幼体的影响
Pub Date : 1900-01-01 DOI: 10.3755/GALAXEA.15.115
Rei Tamai, K. Sakai
Overharvesting of herbivorous fishes is assumed to be one of the causes for phase shift on coral reefs from coralto macroalgal-dominated communities by reducing inhibitor of algal growth. In order to reveal the effect of herbivorous fishes on algae and juvenile acroporid corals, field experiment was conducted in Okinawa, southern Japan. Grazer-exclusion cages were established where small (≈2 cm in length) coral branchlets of Acropora tenuis were transplanted both inside and outside the cages. During the exclusion experiment, algal biomass, survival and growth of the transplanted corals were monitored. The cages effectively excluded herbivorous fishes that resulted in significantly greater algal biomass inside the cages than outside. While algal biomass continued to increase within the cages, algal species composition has changed drastically at the middle of the experimental period. During the first half period (3 months) when encrusting turf algae covered substrate adjacent to coral branchlets inside the cages, coral branchlets could not expand their attachment area on the substrata. In contrast, during the second half period (3 months) corals started rapid growth after turf algae disappeared and frondose macroalgae dominated. We conclude that turf algae may prevent the growth of juvenile acroporid corals especially in the early stages of horizontal expansion prior to the vertical growth.
草食性鱼类的过度捕捞被认为是通过减少藻类生长抑制剂而导致珊瑚大藻群落相移的原因之一。为了揭示草食性鱼类对藻类和拟肢珊瑚幼鱼的影响,在日本南部冲绳进行了野外试验。在笼内和笼外分别移植长约2 cm的鹿角珊瑚小枝,建立放牧隔离笼。在排除试验期间,监测了移植珊瑚的藻类生物量、存活和生长情况。网箱有效地排除了草食性鱼类,导致网箱内的藻类生物量明显高于网箱外。虽然笼内藻类生物量持续增加,但在实验中期,藻类种类组成发生了巨大变化。在前半期(3个月),当结壳的草皮藻类覆盖在笼子内珊瑚小枝附近的基质时,珊瑚小枝不能扩大在基质上的附着面积。后半期(3个月),植藻消失,大型藻占主导地位,珊瑚开始快速生长。我们得出结论,草皮藻类可能会阻止幼螺的生长,特别是在水平扩张的早期阶段,而不是垂直生长。
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引用次数: 3
Genus-specific bleaching at Con Dao Islands, Southern Vietnam, June 2019 2019年6月,越南南部孔岛群岛发生属特异性白化
Pub Date : 1900-01-01 DOI: 10.3755/galaxea.22.1_27
V. Tuấn, P. Hoang, Hua Thai Tuyen, Thai Minh Quang, H. Bền
± 26.1%), Montipora (10.1 ± 5.7%; Fig. 1A). Two genera Galaxea and Diploastrea did not suffer bleaching. Acropora with most branch and tabulate colonies were less impacted both in deep and shalow waters (0.9 ± 1.3% bleached; Fig. 1D - F). Hard corals in deeper waters exhibited a higher susceptibility to bleaching than their shallow counterparts (48% bleached in deeper transects compared with 15% in shallow transects). The findings were consistent with the higher abundance of the five most susceptible genera mentioned above in deeper (total mean cover = 17.5%) than in shallow (2.2%) transects. An earlier bleaching event at Con Dao islands in October 1998 resulted in 37.8% of hard coral colonies bleached (Vo 2000). The 2019 event had both similarities and differences in impacts. Soft corals were the most susceptible in both events (100% bleached) and Galaxea remained consistently unbleached. In 2019, Pachyseris and Fungia had much higher levels of bleaching than in 1998, when these genera were little affected (7.7% and 8.3% bleached). In contrast, Diploastrea had no bleaching compared with 14% bleached in 1998. Porites was severely impacted at both events with 57% bleached and many dead massive corals covered by filamentous algae were recorded in October 1998. Acropora were among the susceptible genera with 19% bleached colonies together with many dead colonies observed in October 1998 (Vo 2000).
±26.1%),Montipora(10.1±5.7%);图1 a)。galaxia属和Diploastrea属没有发生白化。在深水和浅水中,枝状菌落和表状菌落最多的鹿角虫受影响较小(0.9±1.3%);图1D - F)。较深水域的硬珊瑚比浅水域的硬珊瑚对白化的敏感性更高(较深水域的白化率为48%,而较浅水域的白化率为15%)。上述5个最敏感属在较深样带(总平均覆盖面积为17.5%)的丰度高于较浅样带(2.2%)。1998年10月在坚岛群岛发生的较早白化事件导致37.8%的硬珊瑚群白化(2000年Vo)。2019年的事件在影响上既有相似之处,也有不同之处。软珊瑚在这两个事件中最容易受到影响(100%漂白),而Galaxea一直没有漂白。2019年,Pachyseris和Fungia的漂白程度比1998年高得多,当时这些属的漂白程度很小(分别为7.7%和8.3%)。相比之下,1998年白化率为14%,而白化率为14%。在这两次事件中,珊瑚受到严重影响,57%的珊瑚白化,而在1998年10月,亦录得许多死珊瑚被丝状藻类覆盖。1998年10月观察到有19%的菌落白化,并有许多菌落死亡(Vo 2000)。
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引用次数: 1
Reef status in the Mandapam group of Islands, Gulf of Mannar 马纳尔湾曼达帕姆群岛的珊瑚礁状况
Pub Date : 1900-01-01 DOI: 10.3755/GALAXEA.12.65
N. Marimuthu, J. J. Wilson, A. Kumaraguru
Manoli Reef Complex (MRC) and Kurusadai Reef Complex (KRC) of the Gulf of Mannar, southeast coast of India were taken for this study to estimate the biophysical status of the coral reef ecosystems. This study was conducted between the years 2006 and 2008. About 25 sites all around the islands were chosen for this study. Among them, good Live Coral (LC) cover was recorded in fourteen sites. The other categories like Dead coral with algae, Exposed reef and Rubble, Seagrass, Algal assem-blage, Muddy flat and Sandy flat were also considered in this study. The estimated sedimentation rate of this area was higher in seaward sides than the shoreward sides.
以印度东南沿海马纳尔湾的马诺里珊瑚礁复合体(MRC)和库鲁萨岱珊瑚礁复合体(KRC)为研究对象,对珊瑚礁生态系统的生物物理状况进行了评估。这项研究是在2006年到2008年之间进行的。这些岛屿周围大约有25个地点被选为这项研究的地点。其中14个地点录得良好的活珊瑚覆盖。其他类别,如死珊瑚带藻类,暴露的珊瑚礁和碎石,海草,藻类集合,泥地和沙地也在本研究中被考虑。该区向海两侧的沉积速率比滨岸两侧的沉积速率高。
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引用次数: 10
SIDIF: Spatial Information Databases Integration Framework 空间信息数据库集成框架
Pub Date : 1900-01-01 DOI: 10.3755/GALAXEA.15.66
M. Mustafa, Zailani Ms, Zaidi Mz
As common practices, evaluation process is done by special dive task force unit to conduct diving activity to observe on how Artificial Reefs (AR) growth. This evaluation for effectiveness and efficiency of AR development is quite difficult and troublesome. In this paper, the framework architecture for databases integration process using location coordinate data (longitude and latitude) of AR will matches with the fish catches data from identified fish landing port that using SIDIF. With SIDIF, the data of fish catches will be mapped with data on location coordinate of AR as a whole via mapping and integration of these two different databases. Using this methodology, the effectiveness and efficiency of AR development will be achieved as well as the evaluation will be measured.
按照惯例,评估过程由专门的潜水工作小组进行,以观察人工鱼礁的生长情况。对AR开发的有效性和效率进行评估是相当困难和麻烦的。本文利用AR的位置坐标数据(经纬度)进行数据库集成过程的框架体系结构,与利用SIDIF识别的鱼港渔获量数据进行匹配。利用SIDIF,通过绘图和整合这两个不同的数据库,将渔获量数据与AR的位置坐标数据作为一个整体进行映射。使用这种方法,将实现AR开发的有效性和效率,并将对评估进行测量。
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引用次数: 0
Dark survival of Oulastrea crispata 乌拉斯特里亚的黑暗生存
Pub Date : 1900-01-01 DOI: 10.3755/GALAXEA.14.117
V. Denis, J. K. Leung, Chia-Min Hsu, H. Hsieh, W. Tsai, Chaolun Allen Chen
2012, after more than one year in total darkness and being exposed to temperature from 10.7 to 31.3oC, this colony was still alive and visually seemed unaffected by such conditions (Fig. 1a). Moreover, extra-tentacular buddings suggested that this colony continues to grow over this period. Lack of chlorophyll fluorescence (
2012年,经过一年多的完全黑暗和10.7到31.3摄氏度的温度,这个群体仍然活着,并且在视觉上似乎没有受到这些条件的影响(图1a)。此外,触角外的萌芽表明这个群体在这段时间内继续生长。缺乏叶绿素荧光(
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引用次数: 7
Mg/Ca and Sr/Ca ratios in a coral from Koh Chueak, Surat Thani, Thailand 在泰国素叻他尼岛的Koh Chueak珊瑚中Mg/Ca和Sr/Ca比值
Pub Date : 1900-01-01 DOI: 10.3755/GALAXEA.14.63
Wararat Sirianansakul, N. Pumijumnong, T. Mitsuguchi, S. Putchakarn, N. Boontanon
Abstract The Mg/Ca and Sr/Ca ratios were determined for Porites lutea specimens that were collected from Koh Chueak, Surat Thani Province, Thailand, using inductively coupled plasma optical emission spectrometry. The ele­mental analyses were performed from the surface to the interior of the coral specimen along its growth axis at 1­mm intervals, generating the near­monthly resolved time series for the Mg/Ca and Sr/Ca ratios. The ratios demonstrate clear annual cycles, which are assumed to reflect variations in the sea surface temperature (SST). Comparing with the annual maximum and minimum values of SST, the following Mg/Ca -SST and Sr/Ca-SST relations were determined: Mg/Ca (mmol/mol)=−1.72+0.193×SST and Sr/Ca (mmol/mol)=11.83−0.098× SST. These relations are different than those of previously published studies. The reason for this difference is unclear; however, it may be related to vital effects that have been observed for geochemical proxies in coral skeletons and to differences in chemical composition of seawater and in sampling procedure.
摘要:采用电感耦合等离子体发射光谱法测定了采自泰国素叻他尼省Koh Chueak的黄岩样品的Mg/Ca和Sr/Ca比值。从珊瑚标本的表面到内部沿其生长轴以1毫米的间隔进行元素分析,生成近月分辨率的Mg/Ca和Sr/Ca比值时间序列。这些比值显示出明显的年循环,假定这反映了海表温度(SST)的变化。对比海温的年最大值和最小值,得到Mg/Ca -SST和Sr/Ca-SST的关系:Mg/Ca (mmol/mol)=−1.72+0.193×SST, Sr/Ca (mmol/mol)=11.83−0.098× SST。这些关系与之前发表的研究结果不同。造成这种差异的原因尚不清楚;然而,这可能与在珊瑚骨骼中观察到的地球化学代用物的重要影响有关,也可能与海水化学成分和取样程序的差异有关。
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引用次数: 2
Presence of Alveopora tizardi (Scleractinia: Acroporidae) as an epibiont on molluscan shells in an anchialine lake of a raised atoll, Kakaban Island, East Kalimantan, Indonesia: An opportunist or a survivor? 在印度尼西亚东加里曼丹卡卡班岛的一个凸起的环礁上,作为软体动物壳上的表面生物的Alveopora tizardi(核虫纲:Acroporidae)的存在:一个机会主义者还是幸存者?
Pub Date : 1900-01-01 DOI: 10.3755/galaxea.g25-5
T. Tomascik, A. J. Mah
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引用次数: 0
期刊
Galaxea, Journal of Coral Reef Studies
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