Pub Date : 2022-01-01DOI: 10.14744/ejmo.2022.67393
Illy Enne Gomes Pereira
layer that connects vessels to surrounding tissues and controls the ex-Endothelial cells (EC) are key elements of vascularized tissues that form a single-cell layer that connects the vessels to the surrounding tissues. EC participate in the regulation of blood hemostasis, leukocyte homing, acute inflammation, wound healing, and antigen presentation. EC subpopulations are characterized by diverse structures, functions, and molecular profiles. In bone marrow, EC are part of the hematopoietic stem cell vascular and endosteal niche, where they play well-defined roles in hematopoietic stem cell functioning and maintenance, and reside surrounding sinusoids and blood vessels. In the past years, the clinical and pathophysiological roles of EC have been explored due to their contribution to neoangiogenesis and alterations in the vascular endothelium functions in hematological diseases. The present review discusses the EC contribution to pathogenesis of hematological malignancies and their potential use as therapeutic target in these diseases. Abstract
{"title":"Role of Endothelial Cells in Hematological Malignancies","authors":"Illy Enne Gomes Pereira","doi":"10.14744/ejmo.2022.67393","DOIUrl":"https://doi.org/10.14744/ejmo.2022.67393","url":null,"abstract":"layer that connects vessels to surrounding tissues and controls the ex-Endothelial cells (EC) are key elements of vascularized tissues that form a single-cell layer that connects the vessels to the surrounding tissues. EC participate in the regulation of blood hemostasis, leukocyte homing, acute inflammation, wound healing, and antigen presentation. EC subpopulations are characterized by diverse structures, functions, and molecular profiles. In bone marrow, EC are part of the hematopoietic stem cell vascular and endosteal niche, where they play well-defined roles in hematopoietic stem cell functioning and maintenance, and reside surrounding sinusoids and blood vessels. In the past years, the clinical and pathophysiological roles of EC have been explored due to their contribution to neoangiogenesis and alterations in the vascular endothelium functions in hematological diseases. The present review discusses the EC contribution to pathogenesis of hematological malignancies and their potential use as therapeutic target in these diseases. Abstract","PeriodicalId":11831,"journal":{"name":"Eurasian Journal of Medicine and Oncology","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86526899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.14744/ejmo.2021.61180
Kalaiyarasi Dhamodharan
{"title":"Chemomodulatory Effect of Capsaicin Encapsulated Chitosan Nanoparticles on Lipids, Lipoproteins and Glycoprotein Components in DMBA Induced Mammary Carcinogenesis in Rats","authors":"Kalaiyarasi Dhamodharan","doi":"10.14744/ejmo.2021.61180","DOIUrl":"https://doi.org/10.14744/ejmo.2021.61180","url":null,"abstract":"","PeriodicalId":11831,"journal":{"name":"Eurasian Journal of Medicine and Oncology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78178424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.14744/ejmo.2022.71066
Y. Boustany
, and Objectives: Monoglyceride lipase (MGLL), as a prominent metabolic hub, is known to be actively involved in the development of the lipogenic phenotype, which promotes de novo lipid biosynthesis, allowing cancer cells to maintain their growth advantage, continuous proliferation, and metastasis. In this study, we aim to investigate mRNA MGLL expression levels and its sub-cellular localization in non-small cell lung cancer (NSCLC) cell lines, as well as examine the effect of two chemotherapy/targeted therapy agents, cisplatin and crizotinib, on the expression levels of MGLL. Methods: pcDNA3.1(-)-MGLL and pEGFPN1-MGLL constructs were sub-cloned into E.coli DH5a and transfected into NSCLC cell lines for MGLL expression evaluation and sub-cellular localization, using polymerase chain reaction (PCR) and quantitative PCR (qPCR) and fluorescence microscopy, respectively. Parent and cisplatin/crizotinib-resistant cell lines were grown and maintained in adequate media for subsequent MGLL expression analysis. Results: PCR and qPCR results revealed that MGLL was successfully transfected into the H1299 cells and efficiently expressed. Fluorescence microscopy of the pEGFPN1-MGLL transfected cells revealed a cytosolic expression of MGLL. As per the analysis on the effect of cisplatin and crizotinib on MGLL expression, a notable downregulation of MGLL expression was noted in the resistant cell lines. Conclusion: These results provide groundwork for further research on molecules modulating MGLL expression, which may be
{"title":"Molecular Cloning, Expression, and Sub-Cellular Localization of MGLL in NSCLC Cell Lines","authors":"Y. Boustany","doi":"10.14744/ejmo.2022.71066","DOIUrl":"https://doi.org/10.14744/ejmo.2022.71066","url":null,"abstract":", and Objectives: Monoglyceride lipase (MGLL), as a prominent metabolic hub, is known to be actively involved in the development of the lipogenic phenotype, which promotes de novo lipid biosynthesis, allowing cancer cells to maintain their growth advantage, continuous proliferation, and metastasis. In this study, we aim to investigate mRNA MGLL expression levels and its sub-cellular localization in non-small cell lung cancer (NSCLC) cell lines, as well as examine the effect of two chemotherapy/targeted therapy agents, cisplatin and crizotinib, on the expression levels of MGLL. Methods: pcDNA3.1(-)-MGLL and pEGFPN1-MGLL constructs were sub-cloned into E.coli DH5a and transfected into NSCLC cell lines for MGLL expression evaluation and sub-cellular localization, using polymerase chain reaction (PCR) and quantitative PCR (qPCR) and fluorescence microscopy, respectively. Parent and cisplatin/crizotinib-resistant cell lines were grown and maintained in adequate media for subsequent MGLL expression analysis. Results: PCR and qPCR results revealed that MGLL was successfully transfected into the H1299 cells and efficiently expressed. Fluorescence microscopy of the pEGFPN1-MGLL transfected cells revealed a cytosolic expression of MGLL. As per the analysis on the effect of cisplatin and crizotinib on MGLL expression, a notable downregulation of MGLL expression was noted in the resistant cell lines. Conclusion: These results provide groundwork for further research on molecules modulating MGLL expression, which may be","PeriodicalId":11831,"journal":{"name":"Eurasian Journal of Medicine and Oncology","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84174528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Review of Immunotherapy Efficacy in Virus-associated Cancers","authors":"Z. Ozay","doi":"10.14744/ejmo.2022.86807","DOIUrl":"https://doi.org/10.14744/ejmo.2022.86807","url":null,"abstract":"DOI: 10.14744/ejmo.2022.86807 EJMO 2022;6(2):100–110","PeriodicalId":11831,"journal":{"name":"Eurasian Journal of Medicine and Oncology","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90519201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunohistochemical Evaluation of CD10 Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma: A Cross-sectional Study","authors":"G. Sridharan","doi":"10.14744/ejmo.2022.15830","DOIUrl":"https://doi.org/10.14744/ejmo.2022.15830","url":null,"abstract":"DOI: 10.14744/ejmo.2022.15830 EJMO 2022;6(2):137–141","PeriodicalId":11831,"journal":{"name":"Eurasian Journal of Medicine and Oncology","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77296259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Incidence of Composite Lymphomas: Case Studies And disease Management","authors":"A. Sudarikov","doi":"10.14744/ejmo.2022.14882","DOIUrl":"https://doi.org/10.14744/ejmo.2022.14882","url":null,"abstract":"DOI: 10.14744/ejmo.2022.14882 EJMO 2022;6(2):130–136","PeriodicalId":11831,"journal":{"name":"Eurasian Journal of Medicine and Oncology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77740777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.14744/ejmo.2022.29140
M. Bonatti
{"title":"Follow-Up of Ovarian Cancer: Correlation Between Imaging Findings and CA 125 Serum Value","authors":"M. Bonatti","doi":"10.14744/ejmo.2022.29140","DOIUrl":"https://doi.org/10.14744/ejmo.2022.29140","url":null,"abstract":"","PeriodicalId":11831,"journal":{"name":"Eurasian Journal of Medicine and Oncology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82787830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The role of Inflammation in benign salivary glands tumours etiopathogenesis: an evaluation through the blood inflammatory biomarkers","authors":"V. Abbate","doi":"10.14744/ejmo.2022.29857","DOIUrl":"https://doi.org/10.14744/ejmo.2022.29857","url":null,"abstract":"DOI: 10.14744/ejmo.2022.29857 EJMO 2022;6(2):150–155","PeriodicalId":11831,"journal":{"name":"Eurasian Journal of Medicine and Oncology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75299538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.14744/ejmo.2022.88057
Akif Ayaz
to out the importance of the diagnosis of breast by the predisposition genes except BRCA1/2 with multigene testing. Methods: In this study, 232 non-BRCA cases with BC and/or BC family history (FH) were analyzed using the next-gen-eration sequencing method. Twenty-two different pathogenic/likely pathogenic variants were determined in 24 (10.34%) of cases, and these variants were detected in the CHEK2 (7/24, 29.1%), ATM (5/24, 20.8%), MUTYH (3/24, 12.5%), BLM (2/24, 8.3%), WRN 8.3%), TP53 (1/24, 4.1%), BRIP1 (1/24, 4.1%), MSH2 (1/24, 4.1%), NBN (1/24, 4.1%), and PTEN (1/24, 4.1%) genes including three novel variants which were identified in the BLM, ATM , and MSH2 (3/22, 13.6%) genes. Fourteen of 24 (58.3%) cases had BC diagnosis, and 10 of 24 (41.6%) cases had a FH of BC. Conclusion: Among non-BRCA BC and/or BC FH cases, cancer susceptibility gene frequency was 10.34% in this study. CHEK2 and ATM genes had relatively high mutation rates. Abstract
{"title":"Importance of Diagnosis in Breast Cancer with Non-BRCA Pathogenic Germline Variants of Cancer Susceptibility Genes using High-Throughput Sequencing Analysis","authors":"Akif Ayaz","doi":"10.14744/ejmo.2022.88057","DOIUrl":"https://doi.org/10.14744/ejmo.2022.88057","url":null,"abstract":"to out the importance of the diagnosis of breast by the predisposition genes except BRCA1/2 with multigene testing. Methods: In this study, 232 non-BRCA cases with BC and/or BC family history (FH) were analyzed using the next-gen-eration sequencing method. Twenty-two different pathogenic/likely pathogenic variants were determined in 24 (10.34%) of cases, and these variants were detected in the CHEK2 (7/24, 29.1%), ATM (5/24, 20.8%), MUTYH (3/24, 12.5%), BLM (2/24, 8.3%), WRN 8.3%), TP53 (1/24, 4.1%), BRIP1 (1/24, 4.1%), MSH2 (1/24, 4.1%), NBN (1/24, 4.1%), and PTEN (1/24, 4.1%) genes including three novel variants which were identified in the BLM, ATM , and MSH2 (3/22, 13.6%) genes. Fourteen of 24 (58.3%) cases had BC diagnosis, and 10 of 24 (41.6%) cases had a FH of BC. Conclusion: Among non-BRCA BC and/or BC FH cases, cancer susceptibility gene frequency was 10.34% in this study. CHEK2 and ATM genes had relatively high mutation rates. Abstract","PeriodicalId":11831,"journal":{"name":"Eurasian Journal of Medicine and Oncology","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73898879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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