Ethiopian Veterinary Journal最新文献

{"title":"Prevalence, risk factors and antibiogram of Escherichia coli isolated from dogs in Ambo, Gojo and Bako towns of Oromia region, Ethiopia","authors":"E. Gebremedhin, Demiso Merga, E. J. Sarba, Lencho Megersa Marami, G. K. Tola, Solomon Shiferaw Endale","doi":"10.4314/EVJ.V25I1.1","DOIUrl":"https://doi.org/10.4314/EVJ.V25I1.1","url":null,"abstract":"Dogs are a potential reservoir for Escherichia coli and other zoonotic bacterial pathogens posing the risk of infection to humans and other animals. A crosssectional study was used to collect 438 rectal swab samples from apparently healthy dogs of Ambo, Gojo, and Bako towns of West Shewa Zone with the objectives of investigating the prevalence, risk factors and antibiogram of E. coli. A questionnaire survey was administered at the household level to collect data on potential risk factors to acquire E. coli infection. Prevalence was determined by using standard bacteriological culture techniques. Further  confirmation and antimicrobial susceptibility testing of E. coli using the minimum inhibitory concentration (MIC) method were  conducted using Automated Phoenix Machine. The overall prevalence of E. coli was found to be 24.2% (95% confidence interval [CI]:20.26-28.49%). The isolation rate of E. coli was the highest in Bako 31.01%, followed by Gojo 30.99% and Ambo 18.49% towns.  Univariable and multivariable logistic regression analyses revealed that there was a significant association between E. coli prevalence and towns and Kebeles (P<0.05). However, age, sex, the presence of other domestic animals in the household, types of the housing system, educational level of the dogs’ owners, and type of feed provided to the dogs did not significantly correlate with the isolation of E. coli (P > 0.05). E. coli isolates were pan-susceptible to amikacin, ertapenem, imipenem, piperacillin-tazobactam, netilmicin, ampicillin, piperacillin, cefoxitin, ciprofloxacin, levofloxacin, and meropenem. A low level of resistance was found to aztreonam, ceftriaxone, (each 13.95%), cefazolin (22.73%), gentamicin (11.90%), and trimethoprim-sulphamethoxazole (6.97%). E. coli isolates showed multidrug resistance to aztreonam, cefazolin, ceftriaxone, gentamicin, and trimethoprim-sulphamethoxazole (14.0%). This demonstrated E. coli prevalence and moderately low antimicrobial resistance  suggests the possible risk of infection of humans. Therefore, raising public awareness about zoonotic canine disease prevention measures and good hygienic practices are essential.. \u0000Keywords: Antibiogram, Dog, Escherichia coli, Ethiopia, Prevalence, Risk factor","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83220242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Geographical Information System in Animal Disease Surveillance and Control: A Review","authors":"B. Tadesse, A. Amare","doi":"10.4314/EVJ.V25I1.8","DOIUrl":"https://doi.org/10.4314/EVJ.V25I1.8","url":null,"abstract":"Animal disease patterns are changing because of climate change and there is a continuous occurrence of re-emerging and emerging types of diseases. So, new and modern tools are essential for monitoring and surveillance of these diseases. This review was done to give an insight on the applications of GIS in animal disease surveillance, reporting and control. Geographic information systems (GIS) provide the easy access, utilization and manipulation of geospatial information. The advantage of GIS is mapping the many different locations of farms and other facilities with animals on a single map which helps in better monitoring and surveillance. GIS also provides detailed information on disease forecasting, prediction of outbreaks, identification of disease clusters or hotspot, creation of buffer zones and to evaluate different strategies to prevent the spread of infectious diseases. Moreover, it provides an ideal condition for the collection of disease related data and their analyses in relation to population distribution, surrounding social and health services and the natural environmental conditions.","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"321 1","pages":"128-143"},"PeriodicalIF":0.0,"publicationDate":"2021-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75248537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small Ruminant Brucella Sero-prevalence and potential risk factor at Dallo-Manna and HarannaBulluk Districts of Bale Zone, Oromia regional state, Ethiopia","authors":"A. Adem, A. Hiko, Hika Waktole, F. Abunna, G. Ameni, G. Mamo","doi":"10.4314/EVJ.V25I1.5","DOIUrl":"https://doi.org/10.4314/EVJ.V25I1.5","url":null,"abstract":"A cross-sectional study was carried out on randomly sampled 384 animals to assess the occurrence of small ruminant brucellosis and risk factors contributing for the zoonotic potential of the disease at Dallo-Manna and HarannaBulluk districts of Bale Zone. Rose Bengal plate test (RBPT) and complement fixation test (CFT) were used serially. All collected serum samples were subjected to RBPT first and then positive sera with RBPT were further tested for confirmation using CFT. Animal level prevalence of 6.5% and 2.9%, and flock level prevalence of 50% and 22% were recorded by RBPT and CFT respectively. Flock level prevalence at Dallo-Manna is 3.8-fold (95% OR CI = 1.17-12.19) than at Haranna-Bulluk (95% OR CI = 0.32-3.31) but no statistical significant difference (p>0.05). The Chi-square (χ2) statistical analysis indicated that age (χ2=6.18; p<0.05), parity (χ2=0.57; p<0.05), retained fetal membrane (χ2=35.5; p<0.001) and abortion history (χ2=45.1; p<0.001) were associated with Brucella sero-reactors in study areas. Small ruminant with history of retained fetal membrane (OR=3, CI: 3.5227) and small ruminant with abortion history (OR=32, CI: 2.26-462.8) were also found significantly associated with seropostiveity. Questioner survey revealed only 30% of the respondents were aware of the small ruminant brucellosis. Most of them (84%) handle aborted materials with bare hand, 94% of the respondents mix sheep and goat at grazing field and watering point. Traditionally the habit of raw milk consumption is com-","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"25 1","pages":"77-95"},"PeriodicalIF":0.0,"publicationDate":"2021-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85437414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and risk factors of swine tuberculosis in central Ethiopia","authors":"K. Demissie, J. Shiferaw, G. Medhin, A. Zewude, A. Sirak, T. Abayneh, G. Mamo, G. Ameni","doi":"10.4314/evj.v24i2.2","DOIUrl":"https://doi.org/10.4314/evj.v24i2.2","url":null,"abstract":"There is paucity of information in the epidemiology and pathology of tuberculosis in swine though the endemic occurrence of bovine tuberculosis was elucidated in Ethiopia. A cross-sectional study was employed to investigate the epidemiology of Mycobacterium tuberculosis complex and  Mycobacterium avium complex in swine. The study was conducted from September 2016 to December 2017 using single intradermal comparative tuberculin test, gross pathology and histopathology. Tuberculin test was carried out in farmed swine of central Ethiopia while the necropsy at Addis Ababa Abattoirs Enterprise, Ethiopia. Of the total 329 heads of swine tuberculin tested by bovine purified protein derivative antigen, an animal level prevalence of 3% (95% CI: 2-6) and a herd level prevalence of 11% (95% CI: 1-49) were observed at a cut-off value of >2mm. Multivariable logistic regression analysis revealed the protective exposure effect of body condition to tuberculin test positivity in swine (Adjusted OR: 0.06; 95% CI: 0.0-1.1; P: 0.055). Swine were predominantly in close proximity with dairy cattle which is an important risk factor for the transmission of bovine tuberculosis from cattle to swine. Necropsy study of tuberculosis-like lesions in 640 heads of swine revealed an overall lesion prevalence of 4.1 % (95 CI: 2.8-6.0). In conclusion, the present study demonstrated the occurrence of swine tuberculosis in low prevalence in farmed swine and swine slaughtered atAddis Ababa Abattoirs Enterprise in central Ethiopia. Further in-depth study covering larger sample size and wider areas is warranted so as to identify the prevalent species and their zoonotic importance. \u0000Keywords: Abattoir; Epidemiology; Ethiopia; Pathology; Swine; Tuberculosis","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"6 1","pages":"16-34"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72655435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unilateral testicular degeneration in dogs: Effects on spermatozoal characteristics, testis and cauda epididymis","authors":"C. Oguejiofor, K. Anya, Nnaemeka Kingsley Ogbanya","doi":"10.4314/evj.v24i2.9","DOIUrl":"https://doi.org/10.4314/evj.v24i2.9","url":null,"abstract":"Testicular degeneration is an important cause of poor fertility in dogs, but there is little knowledge on its effects on spermatozoa in affected dogs. The study investigated the specific effects of unilateral testicular degeneration (UTD) on spermatozoal characteristics in the testis and epididymis. Ten sexually mature Nigerian indigenous breed of dogs, comprising 5 normal dogs and 5 dogs with UTD were used for the study. The testis and epididymis were removed via orchidectomy for morphological and histopathological evaluation. Sperm in the testis and cauda epididymis were analysed. The samples were grouped into four as N1 (normal right testis), N2 (normal left testis), ND (non-degenerated testis in UTD dogs), and D (degenerated testis in UTD dogs). Data were analysed using one-way ANOVA. There were significant decreases (p<0.001) in testes weight, length, width and volume, and the gonado-somatic index in the D testes compared to the contralateral ND and the N groups. The D group also had significantly lowered epididymal sperm total and progressive motility, viability and concentration (p<0.001), and a decreased testicular sperm  concentration (p<0.01). Moreover, there was a decreased percentage (p<0.001) of morphologically normal sperm, with increased prevalence of  sperm abnormalities in the D group compared to the other groups. In comparison with the N groups, the ND group had a significantly lowered (p<0.05) epididymal sperm progressive motility, with increased percentage (p<0.01) of sperm with proximal cytoplasmic droplets and looped tails. The findings demonstrated that UTD in dogs adversely affected spermatozoa in the testis and cauda epididymis. There was also evidence of compromised spermatozoa in the epididymis contralateral to the degenerated testis. \u0000Keywords: Canine; Epididymis; Spermatozoa; Testicular atrophy; Testicular degeneration","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"9 1","pages":"139-154"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85729561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of wound, its associated risk factors and wound management practices in carthorses of ten selected towns of Ethiopia","authors":"M. Teferi, Reta Tesfaye, Hanna Zewdu, Gizaw Gemechu, G. Tefera, T. Ashine","doi":"10.4314/evj.v24i2.1","DOIUrl":"https://doi.org/10.4314/evj.v24i2.1","url":null,"abstract":"A cross-sectional study was conducted from November 2017 to April 2018 to estimate the prevalence of wound and associated risk factors in carthorses, and to assess wound management practices in 10 selected towns of Ethiopia. The study was conducted through personal interview and observation of carthorses. Randomly selected 390 drivers and their carthorses were included in this study. The study revealed that, the overall prevalence of wound was 51.5% (n=201). The prevalence of wound significantly varied among the study towns. It was high (70%, n =28) in Shashemene and low (30%, n=9) in Sheno. More than one type of wound was the most common (21%, n=82) type to occur. Harness related wounds such as girth sore (20.8%, n=81), chest sore (11.8%, n=46), bit sore (11.8, n=46), saddle sore (7.9%, 31), tail sore (3.1%, n=12) and collar sore (0.5%, n=2) were observed. The non-harness related wounds identified were fetlock sore (12.3%, n=48), knee sore (3.3%, n=13), whip sore (3.1%, n=12) and other sores (1.5%, n=6). Generally, wound related with harnesses were more frequent. The prevalence of wound was observed significantly different between body condition scores. Horses with body condition score 2 were 2 times more likely to be wounded than horses with better body condition score (BCS=3) [OR: 2.4 95%CI (1.36-4.25)]. Wound prevalence was low in horses where owner own only one horse compared to owners keeping two and more horses [OR: 1.56, 95% CI (1.01-2.33)]. Bits with smooth surface were used in less than half (44.4%, n=173) of the observed animals. Variation in frequency of bit sore was observed based on bit types used. The majority of bit sores were caused by metal bits with rough surface (χ2 =10.52, p= 0.03). Majority (83.3%, n=325) of the owners were aware of proper wound management practices. Carthorses often transport 3 to 4 people at a time, the average working","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"1 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74549568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trypanocidal drug utilization practices in tsetse suppression and non-suppression areas of South Omo Zone, Southwestern Ethiopia","authors":"Tegegn Tesfaye, Tekle Olbamo, H. Ashenafi","doi":"10.4314/evj.v24i2.6","DOIUrl":"https://doi.org/10.4314/evj.v24i2.6","url":null,"abstract":"Trypanosomosis control in Ethiopia is largely rely on use of available trypanocidal drugs although there are other options such as vector control and  use of trypanotolerant hosts. A cross-sectional survey aimed at assessing the knowledge, attitude and practices of trypanocidal drug utilization  and constraints of trypanosome infection conducted in tsetse suppression and non-suppression areas of South Omo Zone, Ethiopia. The questionnaire based survey was conducted from November 2018 to May 2019. Descriptive statistics was used to summarize the field data obtained from 184 cattle owners. Sixty (60) of the cattle owners were from suppression area and 124 from tsetse non-suppression area. Accordingly, draft oxen and milking cows respectively from tsetse suppression and non-suppression areas were classes of animals which were given priority in trypanocidal drug treatment. About 79.03% and 81.7% of cattle owners respectively from tsetse suppression and non-suppression areas witnessedthat they treat their sick animals by themselves; indicating that veterinarians and other animal health experts have very little role in medication of sick animals. Diminazine aceturate (DA) was the main trypanocidal drug preferred by cattle owners in tsetse suppression area while both DA and Isometamidium chloride (ISM) were used in non-suppression areas. About 83.1% of the respondents from tsetse suppression areas and 86.7% from non-suppression area reported treatment failures following the use of trypanocidal drugs. Moreover, about 79.61% and 86.53% of respondents respectively from tsetse suppression and non-suppression areas observed that drugs obtained from private drug stores were less effective compared to drugs obtained from governmental veterinary clinics. Furthermore, the respondents disclosed that DA was the most horrible  trypanocidal drug in showing treatment failures despite high preference by cattle owners. It was also noted that treatment frequency was higherin tsetse suppression areas than non-suppression areas regardless of vector suppression campaign. In conclusion, higher dependency of cattle  owners on trypanocidal drugs, limited trypanocidal drug availability in the veterinary pharmaceutical market, frequent trypanocidal drug usage and injection by unskilled herdsmen and owners report on trypanocidal drug treatment failures may point out the issue of trypanocidal drug resistance in the area. Therefore, awareness creation to livestock owners on the effect of misuse of trypanocidal drugs and safe trypanocidal drug usage policy should be put into effect to uphold the effectiveness of currently available trypanocidal drugs. \u0000Keywords: Trypanosomosis; Trypanocidal Drugs; Tsetse suppression; South Omo Zone; Ethiopia","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"14 1","pages":"90-111"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86046340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, organ distribution and antimicrobial susceptibility profile of Salmonella isolated from chickens purchased from markets in selected districts of West Shoa, Ethiopia","authors":"E. J. Sarba, Kebene Kudama, Morka Dandecha, Lencho Megersa Marami, B. Borena, Endrias Zewdu Gebremdhin","doi":"10.4314/evj.v24i2.5","DOIUrl":"https://doi.org/10.4314/evj.v24i2.5","url":null,"abstract":"Salmonella is one of the major causes of heavy losses in chicken and foodborne diseases worldwide. The current study was conducted from  November 2015 to May 2016 to estimate the prevalence of Salmonella and determine the antimicrobial susceptibility of isolates in chickens. Chickens (n=205) were purchased from local markets of five selected districts of West Shoa Zone, Central Ethiopia. Following clinical examination, chicken were euthanized and 2-3 ml of blood sample was collected immediately. Then after postmortem examination, samples were collected from the liver, kidney, ovary, and spleen. The slide agglutination test was used to assess the seroprevalence of Salmonella antibodies. Isolation of Salmonella was performed according to the ISO-6579 procedure. The isolates were subjected to antimicrobial susceptibility testing (using 13 antimicrobial drugs) following the Kirby-Bauer disc diffusion method. The seroprevalence of Salmonella antibodies was 63.5% (95% CI: 55.9-70.5). The isolation rate of Salmonella was 19.0% (95% CI: 13.9-20.1) at the chicken level and 7.3% (95% CI: 5.5-9.4) at the organ level. The detection rate was 11.2%, 7.0%, 6.1%, and 4.4% for spleen, liver, ovary, and kidney, respectively. The majority of the Salmonella isolates were susceptible to norfloxacin (97.4%) and chloramphenicol (92.3%). All the 39 isolates were resistant to amoxicillin, tetracycline, and nitrofurantoin. Three multidrug resistance patterns to six antimicrobial classes were observed. Four isolates were resistant to five antimicrobial classes. Therefore, regular  surveillance of Salmonella and its antimicrobial resistance is needed for a better understanding of the epidemiological dynamics. Awareness creation for chicken farmers about improving farming practices and the risks of antimicrobial resistance warrants special attention. \u0000Keywords: Antimicrobial susceptibility; Chicken; Prevalence; Salmonella; Ethiopia","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"9 1","pages":"73-89"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77106201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fore-Stomach Foreign Bodies: prevalence, associated risk factors and types affecting cattle slaughtered at Gondar ELFORA abattoir, northwest Ethiopia","authors":"Amare Bihon, Teketaye Bayeleyegn, A. Assefa, Y. Muktar","doi":"10.4314/evj.v24i2.7","DOIUrl":"https://doi.org/10.4314/evj.v24i2.7","url":null,"abstract":"In Ethiopia, recurrent drought and feed shortage coupling with high level of environmental pollution predispose the animals to foreign body  ingestion. A cross-sectional study with systematic random sampling approach was employed from November 2018 to April, 2019 with the objectives of estimating the prevalence; identify associated risk factors and to categorize the types of foreign body in cattle slaughtered at Gondar ELFORA Abattoir. Ante-mortem and postmortem examinations were used to collect the data. From the total of 384 animals examined, the overall prevalence of foreign body was 83(21.61%). Adult and old animals were 4.33 (95% CI=0.98, 19.00, p=0.052) and 4.54 (95% CI=1.03, 19.96, p=0.045) times more likely to have a chance of getting foreign body than young animals by keeping another factors constant, respectively. However, the difference is not statistically significant for adult cattle. Moreover, poor and medium body conditioned animals were 2.19 (95% CI=1.04, 4.56, p=0.037) and 1.51 (95% CI=0.72, 3.13, p=0.273) times more likely to acquire foreign bodies than good body conditioned animals by keeping another factors constant. In the positive cases (N=83), 41(49.40%), 34(40.96%), 8(9.64%) and 0(0%) of the foreign bodies were found in the rumen, reticulum, both rumen and reticulum, and omasum, respectively. Majority of foreign bodies identified (79.51%) were non-metallic in nature, including clothes, plastics, rope,  sand and stone. Designing and implementation of appropriate solid waste disposal and management practices are strongly recommended to reduce the risk of ingestion of indigestible foreign bodies. \u0000Keyword: Abattoir; Cattle; Foreign body; Fore stomach; Prevalence","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"308 1","pages":"112-122"},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78260684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian follicular dynamics in Boran and Crossbred heifers in Ethiopia: Implications for assisted reproductive techniques","authors":"Jeilu Jemal, T. Degefa, T. Yilma, Sayid Ali, L. Alemayehu","doi":"10.4314/evj.v24i2.4","DOIUrl":"https://doi.org/10.4314/evj.v24i2.4","url":null,"abstract":"The study was conducted to characterize the follicular dynamics of purebred Boran (Bos indicus) and Boran * Holstein Friesian crossbred heifers  during estrous cycles; for use in ovum pick up and in-vitro embryo production. Insight of reproductive physiology of cattle would help to understand and exploit the reproductive potential of elite animals for breed improvement. Follicular development, growth and atresia during estrous cycles were evaluated using a trans-rectal real-time B-mode ultrasound system for three consecutive estrus cycles. Luteal activity was evaluated by serum progesterone level. Follicular aspirations were done to investigate the potential of Boran cattle and their crosses for transvaginal oocyte production; using a vacuum pressure pump and Aloka SSD Prosound-2 ultrasound device. Boran heifers (n=15) manifested two (n = 6, 40%), three (n = 5, 33%), four (n= 3, 20%) and five (n=1, 6.7%) follicular waves. Crossbred heifers (n=14) showed one (n= 2, 14%), two (n= 6, 43%) and three (n= 6, 43%) follicular waves. Interovulatory interval was 21.1 ± 3.4 and 21.4 ± 2.7 days for Boran (n=45) and crossbred (n=42) heifers, respectively. The progesterone level in Boran and Crossbred heifers during diestrus was 9.5 ± 11.0 and 4.6 ± 8.8 ng/ml, respectively. The maximum diameter of the ovulatory follicle for crossbred heifers was higher (15.4 ± 1.6 mm) than the diameter of the Boran (14.0 ± 1.9 mm) heifers (p<0.005). Differences (p<0.005) were observed in the size of both right (26.6 ± 5.14 and 28.6 ± 5.1 mm) and left (21.7 ± 4.85 and 24.1 ± 5.07 mm) ovaries of Boran and Crossbred heifers, respectively. Difference (p<0.005) was also observed in follicular count of the  right ovaries of Boran (4.84 ± 1.96) and Crossbred  (5.13 ± 2.05) heifers. Oocyte recovery rate in once weekly collection scheme for Boran and their crossbred heifers was (n=19, 42.08%) and (n=17, 42.55%), respectively. The recovery rate for twice weekly collection scheme was (n=24, 34.53%) and (n=23, 40.44%) for Boran and crossbred heifers, respectively. Follicular dynamics in Boran heifers is characterized by a higher incidence of cycles with two, three and four waves, associated with a low persistence of the dominant follicles; and smaller size of ovulatory follicles and less intense heat signs from their crossbred counterpart. Boran heifers proved to have potential for comparable number of follicular population and ease of aspiration procedures that can be tapped for advancedreproductive techniques. \u0000Keywords: Estrus cycle; follicular wave; ovarian follicle; ovum pickup; ultrasound.","PeriodicalId":12019,"journal":{"name":"Ethiopian Veterinary Journal","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76671439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}