In this study, we investigate the physiological response of olive flounder, Paralichthys olivaceus and Japanese croaker, Nibea japonica , on a series of rising water temperatures (WT). At 1, 9, and 17 days, WT increased from 23 ℃ (control) to 26, 29 and 32 ℃ within 6 hours, respectively, and then maintained for 96 hours. At 5, 13, and 21 days, WT decreased from each WT to 23 ℃ within 6 hours and was maintained at 23 ℃ for 96 hours. Blood were collected at 0, 1, 5, 9, 13, 17, 21, and 60 days. In both species, hematocrit, red blood cell and hemoglobin of stressed groups were lower than non-stressed group at 13 days. The osmolality of stressed group was lower than non-stressed group at 21 and 60 days. In olive flounder, hemoglobin of stressed groups was lower than non-stressed group at 21 and 60 days, respectively, but osmolality of stressed group was higher than non-stressed group at 9 days. In Japanese croaker, hematocrit, red blood cell and hemoglobin of stressed groups were higher than non-stressed group at 21 and 60 days, respectively, and osmolality of stressed group was higher than that of non-stressed group at 1 day. At 1, 17, and 21 days, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of stressed group were higher than non-stressed group in olive flounder. In Japanese croaker, AST and ALT of stressed group was lower than non-stressed group at 17, 21, and 60 days. The cortisol of olive flounder increased with increasing WT, but those of Japanese croaker increased with decreasing WT. In both species, the glucose increased with increasing WT. After changing WT, lactic acid of olive flounder and Japanese croaker were not significantly different. The physiological response of olive flounder increased directly to the increase in WT, and Japanese croaker contrasted with olive flounder.
{"title":"Stress response of olive flounder, Paralichthys\u0000 olivaceus and Japanese croaker, Nibea japonica on\u0000 changes of water temperature","authors":"J. Hur","doi":"10.47853/fas.2022.e40","DOIUrl":"https://doi.org/10.47853/fas.2022.e40","url":null,"abstract":"In this study, we investigate the physiological response of olive flounder, Paralichthys olivaceus and Japanese croaker, Nibea japonica , on a series of rising water temperatures (WT). At 1, 9, and 17 days, WT increased from 23 ℃ (control) to 26, 29 and 32 ℃ within 6 hours, respectively, and then maintained for 96 hours. At 5, 13, and 21 days, WT decreased from each WT to 23 ℃ within 6 hours and was maintained at 23 ℃ for 96 hours. Blood were collected at 0, 1, 5, 9, 13, 17, 21, and 60 days. In both species, hematocrit, red blood cell and hemoglobin of stressed groups were lower than non-stressed group at 13 days. The osmolality of stressed group was lower than non-stressed group at 21 and 60 days. In olive flounder, hemoglobin of stressed groups was lower than non-stressed group at 21 and 60 days, respectively, but osmolality of stressed group was higher than non-stressed group at 9 days. In Japanese croaker, hematocrit, red blood cell and hemoglobin of stressed groups were higher than non-stressed group at 21 and 60 days, respectively, and osmolality of stressed group was higher than that of non-stressed group at 1 day. At 1, 17, and 21 days, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of stressed group were higher than non-stressed group in olive flounder. In Japanese croaker, AST and ALT of stressed group was lower than non-stressed group at 17, 21, and 60 days. The cortisol of olive flounder increased with increasing WT, but those of Japanese croaker increased with decreasing WT. In both species, the glucose increased with increasing WT. After changing WT, lactic acid of olive flounder and Japanese croaker were not significantly different. The physiological response of olive flounder increased directly to the increase in WT, and Japanese croaker contrasted with olive flounder.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73217349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. H. Nghia, Nguyen Ngọc Tung, Phan Trong Binh, Lew May, Tong Tran Huy, Pham Thai Giang, S. St-Hilaire, Phan Thi Van
This study assessed the effects of oxygen and ozone nanobubbles on gill morphology, weight gain, and mortality of Pacific white shrimp ( Penaeus vannamei ), as well as the level of Vibrio parahaemolyticus and water quality of shrimp culture tanks under lab conditions. Two experiments were carried out with oxygen macrobubble, ozone macrobubble, oxygen nanobubble, ozone nanobubble, and control treatments (air-stone macrobubble). Experiments were done in triplicate in 100 L tanks with 15‰ saline water, and 20 shrimp per tank. Tanks in Experiment 1 were not inoculated with bacteria; tanks in Experiment 2 were inoculated with V. parahaemolyticus at a concentration of 10 6 CFU/mL. The results revealed that short treatments with ozone nanobubbles had minimal impact on shrimp gills, mortality, and growth rates, reduced V. parahaemolyticus concentration in water compared to the other groups, and improve water quality. These laboratory results indicate that ozone nanobubble treatment may be useful for controlling V. parahaemolyticus . More work is needed to find the best protocol to apply the technology on a commercial scale.
{"title":"Effect of nanobubbles (oxygen, ozone) on the Pacific white shrimp\u0000 (Penaeus vannamei), Vibrio\u0000 parahaemolyticus and water quality under lab\u0000 conditions","authors":"N. H. Nghia, Nguyen Ngọc Tung, Phan Trong Binh, Lew May, Tong Tran Huy, Pham Thai Giang, S. St-Hilaire, Phan Thi Van","doi":"10.47853/fas.2022.e39","DOIUrl":"https://doi.org/10.47853/fas.2022.e39","url":null,"abstract":"This study assessed the effects of oxygen and ozone nanobubbles on gill morphology, weight gain, and mortality of Pacific white shrimp ( Penaeus vannamei ), as well as the level of Vibrio parahaemolyticus and water quality of shrimp culture tanks under lab conditions. Two experiments were carried out with oxygen macrobubble, ozone macrobubble, oxygen nanobubble, ozone nanobubble, and control treatments (air-stone macrobubble). Experiments were done in triplicate in 100 L tanks with 15‰ saline water, and 20 shrimp per tank. Tanks in Experiment 1 were not inoculated with bacteria; tanks in Experiment 2 were inoculated with V. parahaemolyticus at a concentration of 10 6 CFU/mL. The results revealed that short treatments with ozone nanobubbles had minimal impact on shrimp gills, mortality, and growth rates, reduced V. parahaemolyticus concentration in water compared to the other groups, and improve water quality. These laboratory results indicate that ozone nanobubble treatment may be useful for controlling V. parahaemolyticus . More work is needed to find the best protocol to apply the technology on a commercial scale.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82765909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Young-Sang Kim, S. N. S. Reid, Je-Kwang Ryu, Bae-Jin Lee, B. Jeon
A randomized, double-blind, and placebo-controlled clinical study was used to determine the cognitive functions related to working memory (WM) and antioxidant properties of fermented Laminaria japonica (FLJ) on healthy volunteers. Eighty participants were divided into a placebo group (n = 40) and FLJ group (n = 40) that received FLJ (1.5 g/day) for 6 weeks. Memory-re-lated blood indices (brain-derived neurotrophic factor, BDNF; angiotensin-converting enzyme; human growth hormone, HGH; insulin-like growth factor-1, IGF-1) and antioxidant function-related indices (catalase, CAT; malondialdehyde, MDA; 8-oxo-2’-de-oxyguanosine, 8-oxo-dG; thiobarbituric acid reactive substances, TBARS) were determined before and after the trial. In addition, standardized cognitive tests were conducted using the Cambridge Neuropsychological Test Automated Batteries. Furthermore, the Korean Wechsler Adult Intelligence Scale (K-WAIS)-IV, and the Korean version of the Montreal Cognitive Assessment (Mo-CA-K) were used to assess the pre and post intake changes on WM-related properties. According to the results, FLJ significantly increased the level of CAT, BDNF, HGH, and IGF-1. FLJ reduced the level of TBARS, MDA, and 8-oxo-dG in serum. Furthermore, FLJ improved physical activities related to cognitive functions such as K-WAIS-IV, MoCA-K, Paired Associates Learning, and Spatial Working Memory compared to the placebo group. Our results suggest that FLJ is a potential candidate to develop functional materials reflecting its capability to induce antioxidant mechanisms together with WM-related indices.
{"title":"Fermented Laminaria japonica improves working memory and antioxidant defense mechanism in healthy adults: a randomized, double-blind, and placebo-controlled clinical study","authors":"Young-Sang Kim, S. N. S. Reid, Je-Kwang Ryu, Bae-Jin Lee, B. Jeon","doi":"10.47853/fas.2022.e41","DOIUrl":"https://doi.org/10.47853/fas.2022.e41","url":null,"abstract":"A randomized, double-blind, and placebo-controlled clinical study was used to determine the cognitive functions related to working memory (WM) and antioxidant properties of fermented Laminaria japonica (FLJ) on healthy volunteers. Eighty participants were divided into a placebo group (n = 40) and FLJ group (n = 40) that received FLJ (1.5 g/day) for 6 weeks. Memory-re-lated blood indices (brain-derived neurotrophic factor, BDNF; angiotensin-converting enzyme; human growth hormone, HGH; insulin-like growth factor-1, IGF-1) and antioxidant function-related indices (catalase, CAT; malondialdehyde, MDA; 8-oxo-2’-de-oxyguanosine, 8-oxo-dG; thiobarbituric acid reactive substances, TBARS) were determined before and after the trial. In addition, standardized cognitive tests were conducted using the Cambridge Neuropsychological Test Automated Batteries. Furthermore, the Korean Wechsler Adult Intelligence Scale (K-WAIS)-IV, and the Korean version of the Montreal Cognitive Assessment (Mo-CA-K) were used to assess the pre and post intake changes on WM-related properties. According to the results, FLJ significantly increased the level of CAT, BDNF, HGH, and IGF-1. FLJ reduced the level of TBARS, MDA, and 8-oxo-dG in serum. Furthermore, FLJ improved physical activities related to cognitive functions such as K-WAIS-IV, MoCA-K, Paired Associates Learning, and Spatial Working Memory compared to the placebo group. Our results suggest that FLJ is a potential candidate to develop functional materials reflecting its capability to induce antioxidant mechanisms together with WM-related indices.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86807158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Letsoin, J. Dangeubun, D. Y. Syahailatua, S. B. Pratasik
This study aims to know the effect of water-extracted horseradish Moringa oleifera leaf and KW21 fertilizer combination application on the density of Nannochloropsis sp. It was conducted in the Natural Food Laboratory of State Polytechnique, Tual. The experiment used complete randomized design with 5 treatments and 3 replications: A (25% horseradish leaf extract + 75% KW21), B (50% horseradish leaf extract + 50% KW21), C (75% horseradish leaf extract + 25% KW21), D (positive control of 100% KW21), and E (negative control of 100% horseradish leaf extract). Results showed that Treatment C yielded the best result, both the highest density of Nannochloropsis sp. and suitable harvest time.
{"title":"Effect of KW21 and water-extracted horseradish leaf combination on\u0000 Nannochloropsis sp. density in laboratory\u0000 scale","authors":"P. Letsoin, J. Dangeubun, D. Y. Syahailatua, S. B. Pratasik","doi":"10.47853/fas.2022.e37","DOIUrl":"https://doi.org/10.47853/fas.2022.e37","url":null,"abstract":"This study aims to know the effect of water-extracted horseradish Moringa oleifera leaf and KW21 fertilizer combination application on the density of Nannochloropsis sp. It was conducted in the Natural Food Laboratory of State Polytechnique, Tual. The experiment used complete randomized design with 5 treatments and 3 replications: A (25% horseradish leaf extract + 75% KW21), B (50% horseradish leaf extract + 50% KW21), C (75% horseradish leaf extract + 25% KW21), D (positive control of 100% KW21), and E (negative control of 100% horseradish leaf extract). Results showed that Treatment C yielded the best result, both the highest density of Nannochloropsis sp. and suitable harvest time.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72977169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. S. Pratama, Y. Putra, R. Pangestuti, Se-Kwon Kim, E. A. Siahaan
Increased fisheries products have raised by-products that are discarded due to low economic value. In addition, marine by-prod-ucts are still rich in protein and nutritional value that have biological activities and give benefits to human health. Meanwhile, there is raised pressure for sustainability practices in marine industries to reduce waste and minimize the detrimental effect on the environment. Thus, valorization by-products through bioactive peptide mining are crucial. This review focus on various ways to obtain bioactive peptides from marine by-products through protein hydrolysis, for instance chemical hydrolysis (acid and based), biochemical hydrolysis (autolysis and enzymatic hydrolysis), microbial fermentation, and subcritical water hydrolysis. Nevertheless, these processes have benefits and drawbacks which need to be considered. This review also addresses various biological activities that are favorable in pharmaceutical industries, including antioxidant, antihypertensive, anticancer, anti-obe-sity, and other beneficial bioactivities. In addition, some potential marine resources of Indonesia for the marine biopeptide from their by-product or undesired marine commodities would be addressed as well.
{"title":"Bioactive peptides-derived from marine by-products: development,\u0000 health benefits and potential application in biomedicine","authors":"I. S. Pratama, Y. Putra, R. Pangestuti, Se-Kwon Kim, E. A. Siahaan","doi":"10.47853/fas.2022.e33","DOIUrl":"https://doi.org/10.47853/fas.2022.e33","url":null,"abstract":"Increased fisheries products have raised by-products that are discarded due to low economic value. In addition, marine by-prod-ucts are still rich in protein and nutritional value that have biological activities and give benefits to human health. Meanwhile, there is raised pressure for sustainability practices in marine industries to reduce waste and minimize the detrimental effect on the environment. Thus, valorization by-products through bioactive peptide mining are crucial. This review focus on various ways to obtain bioactive peptides from marine by-products through protein hydrolysis, for instance chemical hydrolysis (acid and based), biochemical hydrolysis (autolysis and enzymatic hydrolysis), microbial fermentation, and subcritical water hydrolysis. Nevertheless, these processes have benefits and drawbacks which need to be considered. This review also addresses various biological activities that are favorable in pharmaceutical industries, including antioxidant, antihypertensive, anticancer, anti-obe-sity, and other beneficial bioactivities. In addition, some potential marine resources of Indonesia for the marine biopeptide from their by-product or undesired marine commodities would be addressed as well.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77556856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Morphometric relationships, condition factor (CF) and reproductive biology are significant tools in fish stock management, fish biology, physiology, conservation and ecology. Growth and reproductive strategy of Oreochromis niloticus were studied from 343 fish specimens collected from Lake Shala between January and December 2018. Fish samples ranged from 7.7 cm to 33.0 cm in total length (TL) and from 7.80 g to 708.21 g in total weight (TW) were collected using gillnets of 4, 6, 8, 10, and 12 mm mesh sizes. The length-weight relationship of O. niloticus was TW = 0.0104TL 3.19 , indicating positive allometric growth of the fish. The sex ratio (0.93:1) was insignificant from the ideal fish distribution of 1:1 (χ 2 = 0.47, p > 0.05). Mean CF for males, females and combined sexes was 1.04, 1.06 and 1.05, respectively and statistically insignificant ( p > 0.05). The spawning peak occurred in July (rainy) and February (dry) periods, as defined by ripe females and the breeding season. Absolute mean fecundity was 806 eggs and correlated positively with TL and TW of the fish ( p < 0.05) (F = 0.56TL 2.29 , R 2 = 0.93, p < 0.05; F = 18.83TW 0.67 , R 2 = 0.90, p < 0.05). The study provides the first detailed account of the morphometric relationships and reproductive biology of O. niloticus in Lake Shala, which can be used as baseline information for successive biological-based studies in Soda Lakes of Ethiopia.
{"title":"Aspects of the growth and reproductive biology of Oreochromis\u0000 niloticus (Linnaeus, 1758) in a tropical Soda Lake, Lake Shala,\u0000 Ethiopia","authors":"S. Wagaw, S. Mengistou, A. Getahun","doi":"10.47853/fas.2022.e34","DOIUrl":"https://doi.org/10.47853/fas.2022.e34","url":null,"abstract":"Morphometric relationships, condition factor (CF) and reproductive biology are significant tools in fish stock management, fish biology, physiology, conservation and ecology. Growth and reproductive strategy of Oreochromis niloticus were studied from 343 fish specimens collected from Lake Shala between January and December 2018. Fish samples ranged from 7.7 cm to 33.0 cm in total length (TL) and from 7.80 g to 708.21 g in total weight (TW) were collected using gillnets of 4, 6, 8, 10, and 12 mm mesh sizes. The length-weight relationship of O. niloticus was TW = 0.0104TL 3.19 , indicating positive allometric growth of the fish. The sex ratio (0.93:1) was insignificant from the ideal fish distribution of 1:1 (χ 2 = 0.47, p > 0.05). Mean CF for males, females and combined sexes was 1.04, 1.06 and 1.05, respectively and statistically insignificant ( p > 0.05). The spawning peak occurred in July (rainy) and February (dry) periods, as defined by ripe females and the breeding season. Absolute mean fecundity was 806 eggs and correlated positively with TL and TW of the fish ( p < 0.05) (F = 0.56TL 2.29 , R 2 = 0.93, p < 0.05; F = 18.83TW 0.67 , R 2 = 0.90, p < 0.05). The study provides the first detailed account of the morphometric relationships and reproductive biology of O. niloticus in Lake Shala, which can be used as baseline information for successive biological-based studies in Soda Lakes of Ethiopia.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76364984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In April and June 2014, mortalities of goldfish occurred at Korea. The principal signs included pale gills, severely enlarged and softened spleen and kidney and red liver. Moreover, the histopathological characteristics observed mainly in hematopoietic tissues of kidney, gill lamellae and intestine. The predominant histopathological changes were severe necrosis of hematopoietic tissue. In addition, nucleus exhibiting peripherally displaced chromatin were particularly abundant in the kidney of affected fish. The histological examination led to hypothesize the implication of a virus in the mortality. The hematopoietic necrosis herpesvirus (Cyprinid herpesvirus 2, CyHV-2) DNA polymerase gene successfully detected in DNA extracted from kidney and spleen of affected fish using PCR assay and showed 100% identity with already deposited CyHV-2 DNA polymerase gene in NCBI. Artificial infection trials using affected tissues filtrates gave cumulative mortalities of 30% for virus injected goldfish. In the present study, CyHV-2 was detected and identified as the causative pathogen of the epizootic in goldfish in Korea.
{"title":"Detection of herpesviral hematopoietic necrosis virus (Cyprinid\u0000 herpesvirus 2, CyHV-2) from goldfish, Carassius auratus (L.) in\u0000 Korea","authors":"M. Jung, Je-Won Ryu, C. Nikapitiya, Sung‐Ju Jung","doi":"10.47853/fas.2022.e36","DOIUrl":"https://doi.org/10.47853/fas.2022.e36","url":null,"abstract":"In April and June 2014, mortalities of goldfish occurred at Korea. The principal signs included pale gills, severely enlarged and softened spleen and kidney and red liver. Moreover, the histopathological characteristics observed mainly in hematopoietic tissues of kidney, gill lamellae and intestine. The predominant histopathological changes were severe necrosis of hematopoietic tissue. In addition, nucleus exhibiting peripherally displaced chromatin were particularly abundant in the kidney of affected fish. The histological examination led to hypothesize the implication of a virus in the mortality. The hematopoietic necrosis herpesvirus (Cyprinid herpesvirus 2, CyHV-2) DNA polymerase gene successfully detected in DNA extracted from kidney and spleen of affected fish using PCR assay and showed 100% identity with already deposited CyHV-2 DNA polymerase gene in NCBI. Artificial infection trials using affected tissues filtrates gave cumulative mortalities of 30% for virus injected goldfish. In the present study, CyHV-2 was detected and identified as the causative pathogen of the epizootic in goldfish in Korea.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76969899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thithi Phetchthumrongchai, N. Chuchird, S. Roytrakul, S. Chintong, W. Klaypradit
In this study we investigate physical and chemical characteristics of immature and mature skipjack tuna ( Katsuwonus pelamis ) roes in fresh and dried forms. Fresh roes were studied for histological structure and also dried by three methods: hot air drying (HD), vacuum drying (VD) and freeze drying (FD). The obtained roe powders were analysed for proximate composition, color value, fatty acid composition, amino acid profile, equivalent umami concentration (EUC) and protein pattern. Unyolked oocytes were more common in immature roes, while fully yolked oocytes were more common in mature roes. All dried tuna roes contained high content of protein and lipid (69.31%–70.55% and 11.14%–16.02%, respectively). The powders obtained by FD provided the highest lightness value (L*). The main fatty acid found in all roe powders was docosahexaenoic acid (DHA) (23.49%–27.02%). Glutamic acid, leucine, and aspartic acid were the three most abundant amino acids found in the powders (13.58–14.61, 8.06–8.42, and 7.81–8.39 g/100 g of protein, respectively). The mature roe powder obtained from HD provided the highest EUC value (73.09 g monosodium glutamate/100 g of samples). The protein band at molecular weight of 97 kDa (vitelline) represented the major protein. Therefore, dried tuna roe could be a functional ingredient source of protein and lipid rich in DHA and it also has potential to be used as taste enhancer with umami compound.
{"title":"Physical, chemical composition and umami compound of dried immature\u0000 and mature roes of skipjack tuna (Katsuwonus\u0000 pelamis)","authors":"Thithi Phetchthumrongchai, N. Chuchird, S. Roytrakul, S. Chintong, W. Klaypradit","doi":"10.47853/fas.2022.e35","DOIUrl":"https://doi.org/10.47853/fas.2022.e35","url":null,"abstract":"In this study we investigate physical and chemical characteristics of immature and mature skipjack tuna ( Katsuwonus pelamis ) roes in fresh and dried forms. Fresh roes were studied for histological structure and also dried by three methods: hot air drying (HD), vacuum drying (VD) and freeze drying (FD). The obtained roe powders were analysed for proximate composition, color value, fatty acid composition, amino acid profile, equivalent umami concentration (EUC) and protein pattern. Unyolked oocytes were more common in immature roes, while fully yolked oocytes were more common in mature roes. All dried tuna roes contained high content of protein and lipid (69.31%–70.55% and 11.14%–16.02%, respectively). The powders obtained by FD provided the highest lightness value (L*). The main fatty acid found in all roe powders was docosahexaenoic acid (DHA) (23.49%–27.02%). Glutamic acid, leucine, and aspartic acid were the three most abundant amino acids found in the powders (13.58–14.61, 8.06–8.42, and 7.81–8.39 g/100 g of protein, respectively). The mature roe powder obtained from HD provided the highest EUC value (73.09 g monosodium glutamate/100 g of samples). The protein band at molecular weight of 97 kDa (vitelline) represented the major protein. Therefore, dried tuna roe could be a functional ingredient source of protein and lipid rich in DHA and it also has potential to be used as taste enhancer with umami compound.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87088181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to estimate the smallest size of mature individuals that can be exploited. Fish samples of Coryphaena hippurus were collected from Kalinaun fishermen’s catches in the Molucca Sea. They were sexed, then the fork length (FL) and maturity stage were recorded. Results showed that C. hippurus in the Molucca Sea had a sex ratio of 1:1.94 ( p < 0.05). Males had a length range of 499–831 mm FL and females were in the length range of 481–813 mm FL. Size at first maturity was estimated as 529 mm FL for males with a range of 475–588 mm FL and 405 mm FL for females. This study provided basic information for future management needs of the dolphinfish, especially in the Molucca Sea.
{"title":"Estimation of first maturity size of dolphinfish Coryphaena\u0000 hippurus Linnaeus in the Molucca Sea, North Sulawesi,\u0000 Indonesia","authors":"S. B. Pratasik, F. F. Tilaar, M. Salaki","doi":"10.47853/fas.2022.e32","DOIUrl":"https://doi.org/10.47853/fas.2022.e32","url":null,"abstract":"This study aims to estimate the smallest size of mature individuals that can be exploited. Fish samples of Coryphaena hippurus were collected from Kalinaun fishermen’s catches in the Molucca Sea. They were sexed, then the fork length (FL) and maturity stage were recorded. Results showed that C. hippurus in the Molucca Sea had a sex ratio of 1:1.94 ( p < 0.05). Males had a length range of 499–831 mm FL and females were in the length range of 481–813 mm FL. Size at first maturity was estimated as 529 mm FL for males with a range of 475–588 mm FL and 405 mm FL for females. This study provided basic information for future management needs of the dolphinfish, especially in the Molucca Sea.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84444533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diem Tho Ho, Nameun Kim, Dong-Hyuck Yun, Ki-Hong Kim, Jae-Ok Kim, G. I. Jang, Do-Hyung Kim
Most of the emerging diseases that threaten humans are caused by RNA viruses which are extremely mutable during evolution. The fish RNA virus, viral hemorrhagic septicemia virus (VHSV) can infect a broad range of aquatic animal hosts, but the trans-missibility of VHSV to mammals has not been thoroughly investigated. Therefore, our study aimed to investigate the potential adverse effects of VHSV in mammals. Briefly, the survival of VHSV was determined using only minimum essential media (MEM-2) and mammalian SNU-1411 and hepa-1c1c7s cells at 15 ℃ and 37 ℃ . Mice ( Mus musculus , 27.3 ± 1.9 g) were intravenously injected with VHSV (2.37E+05 TCID 50 · mice –1 ) in triplicate. Clinical signs and survival rates were examined at 14 days post-challenge, and infection was confirmed in the surviving mice. The 50% tissue culture infective dose (TCID 50 ) and polymerase chain reaction analysis were used to determine viral titers and the infection rate, respectively. The titer of VHSV suspended in MEM-2 at 15 ℃ was reduced by only one log after 8 days, whereas the virus maintained at 37 ℃ was inactivated 8 days post-inoculation (dpi). There were no recognizable cytopathic effects in either SNU-1411 or hepa-1c1c7s cells inoculated with VHSV at 15 ℃ and 37 ℃ . VHSV in those cell lines at 37 ℃ was rapidly decreased and eventually inactivated at 12 dpi, whereas virus at 15 ℃ remained at low concentrations without replication. In vivo experiment showed that there were no signs of disease, mortality, or infection in VHSV-infected mice. The results of this study indicate that it is highly unlikely that VHSV can infect mammals including humans.
{"title":"Potential harmful effects of viral hemorrhagic septicemia virus in\u0000 mammals","authors":"Diem Tho Ho, Nameun Kim, Dong-Hyuck Yun, Ki-Hong Kim, Jae-Ok Kim, G. I. Jang, Do-Hyung Kim","doi":"10.47853/fas.2022.e29","DOIUrl":"https://doi.org/10.47853/fas.2022.e29","url":null,"abstract":"Most of the emerging diseases that threaten humans are caused by RNA viruses which are extremely mutable during evolution. The fish RNA virus, viral hemorrhagic septicemia virus (VHSV) can infect a broad range of aquatic animal hosts, but the trans-missibility of VHSV to mammals has not been thoroughly investigated. Therefore, our study aimed to investigate the potential adverse effects of VHSV in mammals. Briefly, the survival of VHSV was determined using only minimum essential media (MEM-2) and mammalian SNU-1411 and hepa-1c1c7s cells at 15 ℃ and 37 ℃ . Mice ( Mus musculus , 27.3 ± 1.9 g) were intravenously injected with VHSV (2.37E+05 TCID 50 · mice –1 ) in triplicate. Clinical signs and survival rates were examined at 14 days post-challenge, and infection was confirmed in the surviving mice. The 50% tissue culture infective dose (TCID 50 ) and polymerase chain reaction analysis were used to determine viral titers and the infection rate, respectively. The titer of VHSV suspended in MEM-2 at 15 ℃ was reduced by only one log after 8 days, whereas the virus maintained at 37 ℃ was inactivated 8 days post-inoculation (dpi). There were no recognizable cytopathic effects in either SNU-1411 or hepa-1c1c7s cells inoculated with VHSV at 15 ℃ and 37 ℃ . VHSV in those cell lines at 37 ℃ was rapidly decreased and eventually inactivated at 12 dpi, whereas virus at 15 ℃ remained at low concentrations without replication. In vivo experiment showed that there were no signs of disease, mortality, or infection in VHSV-infected mice. The results of this study indicate that it is highly unlikely that VHSV can infect mammals including humans.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87473345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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