An organism’s physiological processes and behaviors are regulated by neuropeptides and hormone peptides. The first neuropeptide identified from echinoderms is SALMFamide. The two most well-studied SALMFamide neuropeptides are S1 and S2, which possess myoactivity on apical muscle, tube feet, and the cardiac stomach of starfishes. However, neuropeptide candidates identified from SALMFamide’s precursor protein sequence have not been investigated. This study aims to compare the bioactivity of SALMFamide neuropeptides from the starfish Asterias rubens using various starfish muscle preparations. In this study, the bioactivity of the L-type SALMFamide neuropeptides from the starfish A. rubens , AYHTGLPFamide (SALMFa-A) and the derivative AYHSALMFamide (SALMFa-B) was investigated. The neuropeptides were applied on Asterias amurensis apical muscle, tube feet, which revealed that the neuropeptides exhibit relaxing activity on apical muscle but no activity on tube feet. The native SALMFa-A peptide had lower relaxing activity on the apical muscle compared to the derivative peptide SALMFa-B. The relaxing activity of two neuropeptides also was compared with those on the apical muscle of Patiria pectinifera , which revealed relaxing activity as well as SALMFamide-S1 and S2 neuropeptides. Moreover, the investigation of SALMFa-A and SALMFa-B peptides’ bioactivity on P. pectinifera cardiac stomach muscle also showed slight relaxing activity.
{"title":"Starfish smooth muscle relaxing activity of SALMFamide isotype\u0000 peptide and its analog derived from starfish, Asterias\u0000 rubens","authors":"Anastasia Kubarova, Hye-Jin Go, H. Oh, N. Park","doi":"10.47853/fas.2022.e51","DOIUrl":"https://doi.org/10.47853/fas.2022.e51","url":null,"abstract":"An organism’s physiological processes and behaviors are regulated by neuropeptides and hormone peptides. The first neuropeptide identified from echinoderms is SALMFamide. The two most well-studied SALMFamide neuropeptides are S1 and S2, which possess myoactivity on apical muscle, tube feet, and the cardiac stomach of starfishes. However, neuropeptide candidates identified from SALMFamide’s precursor protein sequence have not been investigated. This study aims to compare the bioactivity of SALMFamide neuropeptides from the starfish Asterias rubens using various starfish muscle preparations. In this study, the bioactivity of the L-type SALMFamide neuropeptides from the starfish A. rubens , AYHTGLPFamide (SALMFa-A) and the derivative AYHSALMFamide (SALMFa-B) was investigated. The neuropeptides were applied on Asterias amurensis apical muscle, tube feet, which revealed that the neuropeptides exhibit relaxing activity on apical muscle but no activity on tube feet. The native SALMFa-A peptide had lower relaxing activity on the apical muscle compared to the derivative peptide SALMFa-B. The relaxing activity of two neuropeptides also was compared with those on the apical muscle of Patiria pectinifera , which revealed relaxing activity as well as SALMFamide-S1 and S2 neuropeptides. Moreover, the investigation of SALMFa-A and SALMFa-B peptides’ bioactivity on P. pectinifera cardiac stomach muscle also showed slight relaxing activity.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81314902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeong-Min Lee, Mi-Jin Yim, Hyun-Soo Kim, Seok-Chun Ko, Ji-Yul Kim, G. Oh, Kyunghwa Baek, Dae-Sung Lee
Research on the potential biological activity of red alga Symphyocladia spp. has been limited to Symphyocladia latiuscula , which is widely used as a food ingredient in Korea. Here, we examined the biological activity of another species, Symphyocladia linearis , which is found in Korea and was reported as a new species in 2013. The aim of this study was to evaluate the antioxidant, anti-inflammatory, and antibacterial properties of a 70% ethanol extract of S. linearis . Antioxidant activity, which was evaluated using radical scavenging assays, revealed half maximal inhibitory concentration values for 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) of 34.57 and 11.70 μg/mL algal extract, respectively. Anti-inflammatory activity of the S. linearis ethanolic extract was evaluated using RAW 264.7 cells by measuring the inhibition of lipopolysaccharide-induced nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) production. The potential cytotoxicity of NO and PGE 2 was first examined, confirming no toxicity at concentrations ranging from 10–100 μg/mL. NO production was inhibited 61.1% and 78.0% at 50 and 100 μg/mL S. linearis extract, respectively; and PGE 2 production was inhibited 69.1%, 83.2%, and 94.8% at 25, 50, and 100 μg/mL S. linearis extract, respectively. Thus, the S. linearis extract showed very strong efficacy against PGE 2 production. The cellular production of reactive oxygen species, measured using 2’,7’-dichlorofluorescin diacetate fluorescence, was inhibited 48.8% by the addition of 100 μg/mL S. linearis extract. Antibacterial activity was evaluated using the disc diffusion method and minimum inhibitory concentration (MIC). S. linearis was effective only against gram-positive bacteria, exhibiting antibacterial activity against Staphylococcus aureus with a MIC of 256 μg/mL extract and against Bacillus cereus with a MIC of 1,024 μg/mL extract. Based on these results, we infer that a 70% ethanolic extract of S. linearis possesses strong anti-inflammatory properties, and therefore has the potential to be used in the prevention and treatment of inflammatory and immune diseases.
{"title":"Antioxidant, anti-inflammatory, and antibacterial activities of a 70%\u0000 ethanol-Symphyocladia linearis extract","authors":"Jeong-Min Lee, Mi-Jin Yim, Hyun-Soo Kim, Seok-Chun Ko, Ji-Yul Kim, G. Oh, Kyunghwa Baek, Dae-Sung Lee","doi":"10.47853/fas.2022.e52","DOIUrl":"https://doi.org/10.47853/fas.2022.e52","url":null,"abstract":"Research on the potential biological activity of red alga Symphyocladia spp. has been limited to Symphyocladia latiuscula , which is widely used as a food ingredient in Korea. Here, we examined the biological activity of another species, Symphyocladia linearis , which is found in Korea and was reported as a new species in 2013. The aim of this study was to evaluate the antioxidant, anti-inflammatory, and antibacterial properties of a 70% ethanol extract of S. linearis . Antioxidant activity, which was evaluated using radical scavenging assays, revealed half maximal inhibitory concentration values for 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) of 34.57 and 11.70 μg/mL algal extract, respectively. Anti-inflammatory activity of the S. linearis ethanolic extract was evaluated using RAW 264.7 cells by measuring the inhibition of lipopolysaccharide-induced nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) production. The potential cytotoxicity of NO and PGE 2 was first examined, confirming no toxicity at concentrations ranging from 10–100 μg/mL. NO production was inhibited 61.1% and 78.0% at 50 and 100 μg/mL S. linearis extract, respectively; and PGE 2 production was inhibited 69.1%, 83.2%, and 94.8% at 25, 50, and 100 μg/mL S. linearis extract, respectively. Thus, the S. linearis extract showed very strong efficacy against PGE 2 production. The cellular production of reactive oxygen species, measured using 2’,7’-dichlorofluorescin diacetate fluorescence, was inhibited 48.8% by the addition of 100 μg/mL S. linearis extract. Antibacterial activity was evaluated using the disc diffusion method and minimum inhibitory concentration (MIC). S. linearis was effective only against gram-positive bacteria, exhibiting antibacterial activity against Staphylococcus aureus with a MIC of 256 μg/mL extract and against Bacillus cereus with a MIC of 1,024 μg/mL extract. Based on these results, we infer that a 70% ethanolic extract of S. linearis possesses strong anti-inflammatory properties, and therefore has the potential to be used in the prevention and treatment of inflammatory and immune diseases.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88558661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Galectins, a family of ß-galactoside-binding lectins, have emerged as soluble mediators in infected cells and pattern recognition receptors (PRRs) responsible for evoking and regulating innate immunity. The present study aimed to evaluate the role of galectin-1 in the host immune response of redlip mullet ( Liza haematocheilia ). We established a cDNA database for redlip mullet, and the cDNA sequence of galectin-1 (LhGal-1) was characterized. In silico analysis was performed, and the spatial and temporal expression patterns in gills and blood in response to lipopolysaccharide polyinosinic:polycytidylic acid, and Lactococcus garvieae were estimated via quantitative real-time PCR. Functional assays were conducted using recombinant protein to investigate carbohydrate binding, bacterial binding, and bacterial agglutination activity. LhGal-1 was composed of 135 amino acids. Conserved motifs (H-NPR, -N- and -W-E-R) within the carbohydrate recognition domain were found in LhGal-1. The tissue distribution revealed that the healthy stomach expressed high levels of LhGal-1. The temporal monitoring of LhGal-1 mRNA expression in the gill and blood showed its significant upregulation in response to immune challenges with different stimulants. rLhGal-1 exhibited binding activity in response to carbohydrates and bacteria. Moreover, the agglutination of rLhGal-1 against Escherichia coli was observed. Collectively, our findings suggest that LhGal-1 may function as a PRR in redlip mullet. Furthermore, LhGal-1 can be considered a significant gene to play a protective role in redlip mullet immune system
半乳糖凝集素是一类ß-半乳糖苷结合凝集素，已在感染细胞和模式识别受体(PRRs)中作为可溶性介质出现，负责唤起和调节先天免疫。本研究旨在探讨半凝集素-1在红唇鲻鱼(Liza haematocheilia)宿主免疫应答中的作用。建立了胭脂鱼的cDNA数据库，确定了半乳糖凝集素-1 (LhGal-1)的cDNA序列。采用硅片分析方法，通过实时荧光定量PCR检测鱼鳃和血液中脂多糖多肌苷、多胞酸和garvieae乳球菌的时空表达模式。利用重组蛋白进行功能分析，研究碳水化合物结合、细菌结合和细菌凝集活性。LhGal-1由135个氨基酸组成。在LhGal-1中发现了碳水化合物识别区域内的保守基序(H-NPR， - n -和- w - e - r)。组织分布显示健康胃高水平表达LhGal-1。对鳃和血液中LhGal-1 mRNA表达的时间监测显示，在不同刺激物的免疫刺激下，LhGal-1 mRNA表达显著上调。rlgal -1对碳水化合物和细菌表现出结合活性。此外，还观察到rlgal -1对大肠杆菌的凝集作用。总之，我们的研究结果表明LhGal-1可能在红唇鲻鱼中起PRR的作用。此外，LhGal-1可以被认为是在红唇鲻鱼免疫系统中发挥重要保护作用的基因
{"title":"Galectin-1 from redlip mullet Liza haematocheilia:\u0000 identification, immune responses, and functional characterization as pattern\u0000 recognition receptors (PRRs) in host immune defense system","authors":"Chaehyeon Lim, H. Kwon, Jehee Lee","doi":"10.47853/fas.2022.e50","DOIUrl":"https://doi.org/10.47853/fas.2022.e50","url":null,"abstract":"Galectins, a family of ß-galactoside-binding lectins, have emerged as soluble mediators in infected cells and pattern recognition receptors (PRRs) responsible for evoking and regulating innate immunity. The present study aimed to evaluate the role of galectin-1 in the host immune response of redlip mullet ( Liza haematocheilia ). We established a cDNA database for redlip mullet, and the cDNA sequence of galectin-1 (LhGal-1) was characterized. In silico analysis was performed, and the spatial and temporal expression patterns in gills and blood in response to lipopolysaccharide polyinosinic:polycytidylic acid, and Lactococcus garvieae were estimated via quantitative real-time PCR. Functional assays were conducted using recombinant protein to investigate carbohydrate binding, bacterial binding, and bacterial agglutination activity. LhGal-1 was composed of 135 amino acids. Conserved motifs (H-NPR, -N- and -W-E-R) within the carbohydrate recognition domain were found in LhGal-1. The tissue distribution revealed that the healthy stomach expressed high levels of LhGal-1. The temporal monitoring of LhGal-1 mRNA expression in the gill and blood showed its significant upregulation in response to immune challenges with different stimulants. rLhGal-1 exhibited binding activity in response to carbohydrates and bacteria. Moreover, the agglutination of rLhGal-1 against Escherichia coli was observed. Collectively, our findings suggest that LhGal-1 may function as a PRR in redlip mullet. Furthermore, LhGal-1 can be considered a significant gene to play a protective role in redlip mullet immune system","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84695468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Photosynthetic bacteria (PSB) attract considerable interest as useful microorganisms; nevertheless, a generalized culture technique has not been previously reported owing to difficulty in their cultivation. Therefore, a simple culture technique suitable for public use was investigated. Among the PSB tested, the strain Rhodobacter azotoformans EBN-7 was the most suitable for scale-up production because it showed the highest specific growth rate (0.20 h –1 ) on basal medium. In scale-up cultivation (500 L), R. azotoformans EBN-7 showed 4.50 × 10 10 colony-forming units mL –1 (number of viable cells), dry cell weight of 26.8 g/L, and a specific growth rate of 0.15 h –1 . Cultivation using this final culture broth (as seed culture) in a 15 L simple reactor was successful, with maintenance of cell activity evident. For use as seed culture, the maximum allowable preservation period of R. azotoformans EBN-7 at 4 ℃ was 3 months. When R. azotoformans EBN-7 cultivated in a simple technique was applied to shrimp aquaculture water, NH 4+ -N was reduced from 0.61 mg/L to 0.24 mg/L (by 60.7%) in 4 days in comparison with the control. Thus, this simple culture technique using R. azotoformans EBN-7 has the potential for a good removal efficiency of NH 4+ -N, making seed culture easier and suitable for public use.
光合细菌(PSB)作为有用的微生物引起了广泛的关注;然而，由于其培养困难，以前尚未报道过一种通用的培养技术。因此，研究了一种适合大众使用的简易培养技术。在所测试的PSB菌株中，固氮成型红杆菌EBN-7在基础培养基上的比生长率最高(0.20 h -1)，最适合规模化生产。放大培养(500 L)时，偶氮成形瘤胃EBN-7菌落形成单位为4.50 × 10 10 mL -1(活细胞数)，干细胞重为26.8 g/L，比生长速率为0.15 h -1。在15l简易反应器中使用最终培养液(作为种子培养物)进行培养是成功的，细胞活性得到了明显的维持。在4℃条件下，固氮菌EBN-7的最大允许保存期为3个月。在对虾养殖水体中施用简单培养的偶氮型瘤胃EBN-7，与对照相比，4 d内nh4 + -N由0.61 mg/L降至0.24 mg/L，降幅达60.7%。由此可见，利用固氮成型R. EBN-7进行简单培养，具有较好的nh4 + -N去除率，使种子培养更容易，适合公众使用。
{"title":"A simple culture technique of Rhodobacter\u0000 azotoformans EBN-7 for public use: application to\u0000 NH4+-N removal in shrimp aquaculture\u0000 water","authors":"K. Cho, Joong Kyun Kim","doi":"10.47853/fas.2022.e47","DOIUrl":"https://doi.org/10.47853/fas.2022.e47","url":null,"abstract":"Photosynthetic bacteria (PSB) attract considerable interest as useful microorganisms; nevertheless, a generalized culture technique has not been previously reported owing to difficulty in their cultivation. Therefore, a simple culture technique suitable for public use was investigated. Among the PSB tested, the strain Rhodobacter azotoformans EBN-7 was the most suitable for scale-up production because it showed the highest specific growth rate (0.20 h –1 ) on basal medium. In scale-up cultivation (500 L), R. azotoformans EBN-7 showed 4.50 × 10 10 colony-forming units mL –1 (number of viable cells), dry cell weight of 26.8 g/L, and a specific growth rate of 0.15 h –1 . Cultivation using this final culture broth (as seed culture) in a 15 L simple reactor was successful, with maintenance of cell activity evident. For use as seed culture, the maximum allowable preservation period of R. azotoformans EBN-7 at 4 ℃ was 3 months. When R. azotoformans EBN-7 cultivated in a simple technique was applied to shrimp aquaculture water, NH 4+ -N was reduced from 0.61 mg/L to 0.24 mg/L (by 60.7%) in 4 days in comparison with the control. Thus, this simple culture technique using R. azotoformans EBN-7 has the potential for a good removal efficiency of NH 4+ -N, making seed culture easier and suitable for public use.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74740163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Endobiotic microalgae inhabit various groups of organisms, including bivalves. In this group, the association between the giant molluscs Tridacna and Symbiodinium is one of the most recognizable. This consortium allows hydrobionts to survive in oligotrophic waters by regulating their metabolism. The available research has provided an understanding of the interaction and adaptation of these symbionts, but the problem of the beginning of the formation of these relationships remains unresolved. In the case of Tridacninae, symbiosis is essential for the survival of bivalves, in contrast to representatives of the Mytilidae and the Coccomyxa found in them. A few works devoted mainly to the morphological aspects of invasion have shown that endobiont causes inflammation and pathology. Having data to clarify the exact “diagnosis” of the interaction of these organisms is not enough. It is possible that the relationship between bivalves and Coccomyxa is in the early stages of being established, which may lead to mutualism or parasitism in the future. We assume that the analysis of works on the symbiosis of Symbiodinium and bivalves will facilitate the course of research for the less studied Coccomyxa and their hosts. By postulating the Coccomyxa represent a unique evolutionary model for the formation of a symbiotic system, it is possible to use this system to study the interaction of organisms during their initial contact. The identified signalling pathways and mechanisms that allow the photobionts to evade host immunity can be useful for constructing new forms of symbiosystems.
{"title":"Endobiotic microalgae in molluscan life","authors":"Y. Sokolnikova","doi":"10.47853/fas.2022.e45","DOIUrl":"https://doi.org/10.47853/fas.2022.e45","url":null,"abstract":"Endobiotic microalgae inhabit various groups of organisms, including bivalves. In this group, the association between the giant molluscs Tridacna and Symbiodinium is one of the most recognizable. This consortium allows hydrobionts to survive in oligotrophic waters by regulating their metabolism. The available research has provided an understanding of the interaction and adaptation of these symbionts, but the problem of the beginning of the formation of these relationships remains unresolved. In the case of Tridacninae, symbiosis is essential for the survival of bivalves, in contrast to representatives of the Mytilidae and the Coccomyxa found in them. A few works devoted mainly to the morphological aspects of invasion have shown that endobiont causes inflammation and pathology. Having data to clarify the exact “diagnosis” of the interaction of these organisms is not enough. It is possible that the relationship between bivalves and Coccomyxa is in the early stages of being established, which may lead to mutualism or parasitism in the future. We assume that the analysis of works on the symbiosis of Symbiodinium and bivalves will facilitate the course of research for the less studied Coccomyxa and their hosts. By postulating the Coccomyxa represent a unique evolutionary model for the formation of a symbiotic system, it is possible to use this system to study the interaction of organisms during their initial contact. The identified signalling pathways and mechanisms that allow the photobionts to evade host immunity can be useful for constructing new forms of symbiosystems.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75345089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Over production of methylglyoxal (MGO) a highly reactive dicarbonyl compound, has been associated in progressive diabetes with vascular complication. Therefore, we investigated whether hot water extract of Loliolus beka meat (LBM-HWE) presents a preserve effect against MGO-induced cellular damage in human umbilical vein endothelial cells (HUVECs). The LBM-HWE extract showed to inhibit MGO-induced cytotoxicity. Additionally, the LBM-HWE reduced mRNA expression of pro-inflammatory cytokines, and reduced MGO-induced advanced glycation end product (AGEs) formation. Furthermore, LBM-HWE induced glyoxalase-1 mRNA expression and reduced MGO-induced carbonyl protein formation in HUVECs. The results implicate that LBM-HWE has protective ability against MGO-induced HUVECs toxicity by preventing AGEs formation. In conclusion, LBM-HWE could be used as a potential treatment material for the prevention of vascular complications of diabetes.
{"title":"Hot water extract of Loliolus beka attenuates\u0000 methylglyoxal-induced advanced glycation end products formation in human\u0000 umbilical vein endothelial cells","authors":"Seon-Heui Cha, H. Jun","doi":"10.47853/fas.2022.e46","DOIUrl":"https://doi.org/10.47853/fas.2022.e46","url":null,"abstract":"Over production of methylglyoxal (MGO) a highly reactive dicarbonyl compound, has been associated in progressive diabetes with vascular complication. Therefore, we investigated whether hot water extract of Loliolus beka meat (LBM-HWE) presents a preserve effect against MGO-induced cellular damage in human umbilical vein endothelial cells (HUVECs). The LBM-HWE extract showed to inhibit MGO-induced cytotoxicity. Additionally, the LBM-HWE reduced mRNA expression of pro-inflammatory cytokines, and reduced MGO-induced advanced glycation end product (AGEs) formation. Furthermore, LBM-HWE induced glyoxalase-1 mRNA expression and reduced MGO-induced carbonyl protein formation in HUVECs. The results implicate that LBM-HWE has protective ability against MGO-induced HUVECs toxicity by preventing AGEs formation. In conclusion, LBM-HWE could be used as a potential treatment material for the prevention of vascular complications of diabetes.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73665480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The first specimen (54.8 mm standard length) of Ernogrammus zhirmunskii Markevich & Kharin, 2011, belonging to the family Stichaeidae, was recorded in Dokdo, East Sea, Korea on July 26, 2021. This species was characterized by a single ventral later-al-line canal from the posterior margin of the pelvic-fin base extending to the anus and one or two rigid spines on the posterior part of the anal fin. This species is similar to Ernogrammus hexagrammus and Ernogrammus walkeri but differs in the number of ventral lateral-line canal present, with E. zhirmunskii consisting of one (unpaired) ventral lateral-line canal compared to other two Ernogrammus species, which have a pair of parallel ventral lateral-line canal. For further analysis of species identification, a partial gene sequence from the mitochondrial DNA cytochrome oxidase subunit I (554 bp) of E. zhirmunskii was obtained for the first time. This study documents the first record of E. zhirmunskii in Korean waters and proposes the new Korean name of ‘Il-gob-jul-be-do-la-chi’ for the species.
{"title":"First report of Ernogrammus zhirmunskii Markevich\u0000 & Kharin, 2011 (Perciformes: Stichaeidae) in Dokdo, Korea","authors":"S. Myoung, Min S. Woo, W. Min, J. Park","doi":"10.47853/fas.2022.e44","DOIUrl":"https://doi.org/10.47853/fas.2022.e44","url":null,"abstract":"The first specimen (54.8 mm standard length) of Ernogrammus zhirmunskii Markevich & Kharin, 2011, belonging to the family Stichaeidae, was recorded in Dokdo, East Sea, Korea on July 26, 2021. This species was characterized by a single ventral later-al-line canal from the posterior margin of the pelvic-fin base extending to the anus and one or two rigid spines on the posterior part of the anal fin. This species is similar to Ernogrammus hexagrammus and Ernogrammus walkeri but differs in the number of ventral lateral-line canal present, with E. zhirmunskii consisting of one (unpaired) ventral lateral-line canal compared to other two Ernogrammus species, which have a pair of parallel ventral lateral-line canal. For further analysis of species identification, a partial gene sequence from the mitochondrial DNA cytochrome oxidase subunit I (554 bp) of E. zhirmunskii was obtained for the first time. This study documents the first record of E. zhirmunskii in Korean waters and proposes the new Korean name of ‘Il-gob-jul-be-do-la-chi’ for the species.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84161753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yukyung Choi, Joohyun Kang, Ye-Kyung Lee, Y. Seo, Sejeong Kim, J. Ha, H. Oh, Yujin Kim, Eun Sug Park, Heeyoung Lee, Soomin Lee, M. Rhee, Y. Yoon
In this study, a microbial risk assessment was performed for the bacteria Vibrio parahaemolyticus , which causes a foodborne illness following the consumption of Jeotgal , a fermented seafood in South Korea. The assessment comprised of six stages: product, market, home, consumption, dose-response, and risk. The initial contamination level (IC) was calculated based on the prevalence of V. parahaemolyticus in 90 Jeotgal samples. The kinetic behavior of V. parahaemolyticus was described using predictive models. The data on transportation conditions from manufacturer to market and home were collected through personal communication and from previous studies. Data for the Jeotgal consumption status were obtained, and an appropriate probability distribution was established. The simulation models responding to the scenario were analyzed using the @RISK program. The IC of V. parahaemolyticus was estimated using beta distribution [Beta (1, 91)]. The cell counts during transportation were estimated using Weibull and polynomial models [δ = 1 / (0.0718 – 0.0097 × T + 0.0005 × T 2 )], while the probability distributions for time and temperature were estimated using Pert, Weibull, Uniform, and LogLogistic distributions. Daily average consumption amounts were assessed using the Pareto distribution [0.60284,1.32,Risk Truncate(0,155)]. The results indicated that the risk of V. parahaemolyticus infection through Jeotgal consumption is low in South Korea. simulation. The risk of V. parahaemolyticus infection in Jeotgal was very low, as analyzed by quantitative risk assessment, with respect to the distribution in South Korea. The result suggested that the low contamination level and distribution environment could be important factors to decrease the probability of V. parahaemolyticus -associated foodborne illnesses.
{"title":"Quantitative microbial risk assessment indicates very low risk for\u0000 Vibrio parahaemolyticus foodborne illness from\u0000 Jeotgal in South Korea","authors":"Yukyung Choi, Joohyun Kang, Ye-Kyung Lee, Y. Seo, Sejeong Kim, J. Ha, H. Oh, Yujin Kim, Eun Sug Park, Heeyoung Lee, Soomin Lee, M. Rhee, Y. Yoon","doi":"10.47853/fas.2022.e42","DOIUrl":"https://doi.org/10.47853/fas.2022.e42","url":null,"abstract":"In this study, a microbial risk assessment was performed for the bacteria Vibrio parahaemolyticus , which causes a foodborne illness following the consumption of Jeotgal , a fermented seafood in South Korea. The assessment comprised of six stages: product, market, home, consumption, dose-response, and risk. The initial contamination level (IC) was calculated based on the prevalence of V. parahaemolyticus in 90 Jeotgal samples. The kinetic behavior of V. parahaemolyticus was described using predictive models. The data on transportation conditions from manufacturer to market and home were collected through personal communication and from previous studies. Data for the Jeotgal consumption status were obtained, and an appropriate probability distribution was established. The simulation models responding to the scenario were analyzed using the @RISK program. The IC of V. parahaemolyticus was estimated using beta distribution [Beta (1, 91)]. The cell counts during transportation were estimated using Weibull and polynomial models [δ = 1 / (0.0718 – 0.0097 × T + 0.0005 × T 2 )], while the probability distributions for time and temperature were estimated using Pert, Weibull, Uniform, and LogLogistic distributions. Daily average consumption amounts were assessed using the Pareto distribution [0.60284,1.32,Risk Truncate(0,155)]. The results indicated that the risk of V. parahaemolyticus infection through Jeotgal consumption is low in South Korea. simulation. The risk of V. parahaemolyticus infection in Jeotgal was very low, as analyzed by quantitative risk assessment, with respect to the distribution in South Korea. The result suggested that the low contamination level and distribution environment could be important factors to decrease the probability of V. parahaemolyticus -associated foodborne illnesses.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74021038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. H. Mohd, M. Thiyahuddin, M. A. A. Rahman, Tan Chun Hong, Hii Yii Siang, N. Othman, A. Rahman, A. R. A. Rahman, A. Fitriadhy
To have a better understanding of the impact of the PETRONAS oil and gas platform on commercial fisheries activities, Universiti Malaysia Terengganu (UMT) examined two approaches which are data collection from satellite and data collection from fishermen and anglers. By profiling the anglers who utilize reefed oil and gas structures for fishing, it can determine if the design and location of the reef platforms will benefit or negatively impacts those anglers and fisherman. Furthermore, this assessment will be contributing to the knowledge regarding the value of offshore oil and gas platforms as fisheries resources. Collectively, the apparent fishing activity data included, combined with the findings in the reefing viability index will help to inform PETRONAS’s future decommissioning decisions and may help determine if the design and proposed locations for future rigs-to-reefs candidates would benefit commercial fishing groups, further qualifying them as appropriate artificial reef candidates. The method applied in this study is approaching by using a data satellite known as Google’s Global Fishing Watch technology, which is one of the applications to measure commercial fishing efforts around the globe. The apparent commercial fishing effort around the selected twelve PETRONAS platforms was analyzed from January 2012 to December 2018. Using the data collection from fishermen which is the total estimation of commercial fish value cost (in Malaysia ringgit, MYR [RM]) in Peninsular Malaysia Asset, Sabah Asset, and Sarawak Operation region. The data were extracted every month from 2016 to 2018 from the National Oceanic and Atmospheric Administration database. Most of the selected platforms that show a high frequency of vessels around the year are platform KP-A, platform BG-A and platform PL-B. The estimated values of commercial fishes varied between platforms, with ranged from RM 10,209.92 to RM 89,023.78. Thus, platforms with high commercial fish value are selected for reefing in-situ and will serve multi-purposes and benefit the locals as well as the country. The current study has successfully assessed the potential reefing area of the Malaysian offshore environment with greater representativeness and this paper focused on its potential as a new fishing ground.
{"title":"Commercial fishery assessment of Malaysian water offshore\u0000 structure","authors":"M. H. Mohd, M. Thiyahuddin, M. A. A. Rahman, Tan Chun Hong, Hii Yii Siang, N. Othman, A. Rahman, A. R. A. Rahman, A. Fitriadhy","doi":"10.47853/fas.2022.e43","DOIUrl":"https://doi.org/10.47853/fas.2022.e43","url":null,"abstract":"To have a better understanding of the impact of the PETRONAS oil and gas platform on commercial fisheries activities, Universiti Malaysia Terengganu (UMT) examined two approaches which are data collection from satellite and data collection from fishermen and anglers. By profiling the anglers who utilize reefed oil and gas structures for fishing, it can determine if the design and location of the reef platforms will benefit or negatively impacts those anglers and fisherman. Furthermore, this assessment will be contributing to the knowledge regarding the value of offshore oil and gas platforms as fisheries resources. Collectively, the apparent fishing activity data included, combined with the findings in the reefing viability index will help to inform PETRONAS’s future decommissioning decisions and may help determine if the design and proposed locations for future rigs-to-reefs candidates would benefit commercial fishing groups, further qualifying them as appropriate artificial reef candidates. The method applied in this study is approaching by using a data satellite known as Google’s Global Fishing Watch technology, which is one of the applications to measure commercial fishing efforts around the globe. The apparent commercial fishing effort around the selected twelve PETRONAS platforms was analyzed from January 2012 to December 2018. Using the data collection from fishermen which is the total estimation of commercial fish value cost (in Malaysia ringgit, MYR [RM]) in Peninsular Malaysia Asset, Sabah Asset, and Sarawak Operation region. The data were extracted every month from 2016 to 2018 from the National Oceanic and Atmospheric Administration database. Most of the selected platforms that show a high frequency of vessels around the year are platform KP-A, platform BG-A and platform PL-B. The estimated values of commercial fishes varied between platforms, with ranged from RM 10,209.92 to RM 89,023.78. Thus, platforms with high commercial fish value are selected for reefing in-situ and will serve multi-purposes and benefit the locals as well as the country. The current study has successfully assessed the potential reefing area of the Malaysian offshore environment with greater representativeness and this paper focused on its potential as a new fishing ground.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90512635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Kasmiati, Andi Tenri Nurunnisa, Amran Amran, Muhammad Ikhwan Resya, M. H. Rahmi
This study aimed to determine the antibacterial activity and toxicity of methanol and hexane extracts of Halymenia durvillei red seaweed which were found abundantly in Kayangan island, South Sulawesi. The antibacterial activity of the crude extract was tested against five gram-negative bacteria, namely Escherichia coli , Salmonella typhi , Pseudomonas aeruginosa , Aeromonas hydrophila , and Vibrio harveyi at a dose of 200 g/disk. Extract toxicity was tested on Artemia salina larvae at concentrations of 1,000, 500, 250, 125, 62.5, and 31.25 µg/mL. The results showed that the methanol and hexane extracts of H. durvillei had the highest activity against S. thypi and A. hydrophila , respectively, with inhibition zones of 26.2 mm and 21.0 mm. On the other hand, the two extracts did not show activity against E. coli and P. aeruginosa , respectively. The toxicity of the methanol extract of H. durvillei was twice as high as that of the hexane extract with half-maximal inhibitory concentration of 98.24 and 184.21 µg/mL, respectively. Thus, the methanol and hexane extracts of red seaweed H. durvillei have the potential as new antibacterial agents respectively against the pathogenic bacteria S. typhi and A. hydrophila , but also have the opportunity to be developed into antitumor herbal compounds.
{"title":"Antibacterial activity and toxicity of Halymenia\u0000 durvillei red seaweed from Kayangan island, South Sulawesi,\u0000 Indonesia","authors":"K. Kasmiati, Andi Tenri Nurunnisa, Amran Amran, Muhammad Ikhwan Resya, M. H. Rahmi","doi":"10.47853/fas.2022.e38","DOIUrl":"https://doi.org/10.47853/fas.2022.e38","url":null,"abstract":"This study aimed to determine the antibacterial activity and toxicity of methanol and hexane extracts of Halymenia durvillei red seaweed which were found abundantly in Kayangan island, South Sulawesi. The antibacterial activity of the crude extract was tested against five gram-negative bacteria, namely Escherichia coli , Salmonella typhi , Pseudomonas aeruginosa , Aeromonas hydrophila , and Vibrio harveyi at a dose of 200 g/disk. Extract toxicity was tested on Artemia salina larvae at concentrations of 1,000, 500, 250, 125, 62.5, and 31.25 µg/mL. The results showed that the methanol and hexane extracts of H. durvillei had the highest activity against S. thypi and A. hydrophila , respectively, with inhibition zones of 26.2 mm and 21.0 mm. On the other hand, the two extracts did not show activity against E. coli and P. aeruginosa , respectively. The toxicity of the methanol extract of H. durvillei was twice as high as that of the hexane extract with half-maximal inhibitory concentration of 98.24 and 184.21 µg/mL, respectively. Thus, the methanol and hexane extracts of red seaweed H. durvillei have the potential as new antibacterial agents respectively against the pathogenic bacteria S. typhi and A. hydrophila , but also have the opportunity to be developed into antitumor herbal compounds.","PeriodicalId":12249,"journal":{"name":"Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89968098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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