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Three-dimensional complex architectures observed in shock processed amino acid mixtures 在震荡处理的氨基酸混合物中观察到三维复杂结构
Pub Date : 2022-02-09 DOI: 10.1017/exp.2021.17
Surendra V. Singh, Jayaram Vishakantaiah, J. K. Meka, M. Muruganantham, Vijay Thiruvenkatam, Vijayan Sivaprahasam, B. N. Rajasekhar, A. Bhardwaj, Nigel J. Mason, B. Sivaraman
Abstract Asteroid and cometary impacts have been considered one of the possible routes for exogenous delivery of organics to the early Earth. It is well established that amino acids can be synthesized due to impact-driven shock processesing of simple molecules and that amino acids can survive the extreme conditions of impact events. In the present study, we simulate impact-induced shock conditions utilizing a shock tube that can maintain a reflected shock temperature of about 5,500 K for 2 ms time scale. We have performed shock processing of various combinations of amino acids with subsequent morphological analysis carried out using Scanning Electron Microscope (SEM), revealing that the shock processed amino acids demonstrate an extensive range of complex structures. These results provide evidence for the further evolution of amino acids in impact-induced shock environments leading to the formation of complex structures and thus providing a pathway for the origin of life.
小行星和彗星的撞击被认为是早期地球上有机物外源传递的可能途径之一。氨基酸可以通过简单分子的冲击加工合成,并且氨基酸可以在冲击事件的极端条件下存活。在本研究中,我们利用一个能在2 ms时间尺度内保持约5,500 K反射冲击温度的激波管来模拟冲击诱导的冲击条件。我们对氨基酸的各种组合进行了冲击处理,随后使用扫描电子显微镜(SEM)进行了形态分析,揭示了冲击处理的氨基酸显示出广泛的复杂结构。这些结果为氨基酸在撞击诱发的冲击环境中进一步进化导致复杂结构的形成提供了证据,从而为生命的起源提供了途径。
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引用次数: 2
Relaxation of the parameter independence assumption in the bootComb R package bootComb R包中参数独立性假设的松弛
Pub Date : 2022-02-09 DOI: 10.1017/exp.2022.13
M. Henrion
Abstract Background The bootComb R package allows researchers to derive confidence intervals with correct target coverage for arbitrary combinations of arbitrary numbers of independently estimated parameters. Previous versions (<1.1.0) of bootComb used independent bootstrap sampling and required that the parameters themselves are independent—an unrealistic assumption in some real-world applications. Findings Using Gaussian copulas to define the dependence between parameters, the bootComb package has been extended to allow for dependent parameters. Implications The updated bootComb package can now handle cases of dependent parameters, with users specifying a correlation matrix defining the dependence structure. While in practice it may be difficult to know the exact dependence structure between parameters, bootComb allows running sensitivity analyses to assess the impact of parameter dependence on the resulting confidence interval for the combined parameter. Availability bootComb is available from the Comprehensive R Archive Network (https://CRAN.R-project.org/package=bootComb).
bootComb R包允许研究人员为任意数量的独立估计参数的任意组合导出具有正确目标覆盖率的置信区间。以前版本(<1.1.0)的bootComb使用独立的bootstrap采样,并且要求参数本身是独立的——这在一些实际应用中是不现实的假设。使用高斯copula来定义参数之间的依赖关系,bootComb包已经扩展到允许依赖参数。更新后的bootComb包现在可以处理依赖参数的情况,用户可以指定定义依赖结构的相关矩阵。虽然在实践中可能很难知道参数之间的确切依赖结构,但bootComb允许运行灵敏度分析来评估参数依赖性对组合参数的结果置信区间的影响。可用性bootComb可从综合R档案网络(https://CRAN.R-project.org/package=bootComb)获得。
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引用次数: 0
Cyclic hardening/softening experimental data in nano-clay-composite and aluminum alloy under high-temperature strain-controlled loading 高温应变控制载荷下纳米粘土-复合材料和铝合金循环硬化/软化实验数据
Pub Date : 2022-02-09 DOI: 10.1017/exp.2021.32
M. Azadi, H. Bahmanabadi, F. Gruen, G. Winter, B. Seisenbacher
Abstract This article presents cyclic hardening/softening behaviors (experimental data) of the heat-treated aluminum-matrix nano-clay-composite (AlSi_N_HT6), compared to those of the piston aluminum alloy (AlSi) under strain-controlled loading. For such an objective, standard samples were fabricated by gravity and stir-casting methods. Low-cycle fatigue experiments were carried out under different strain amplitudes (0.20–0.45%) and at various temperatures (25–300°C). Obtained results implied that no obvious change was observed on material properties of aluminum alloy by reinforcements, but a decrement was observed due to increasing the temperature. Results also indicated that the increase of the temperature from 25°C to 200°C has changed the cyclic behavior of both materials (AlSi_N_HT6 and AlSi) from hardening to softening. Moreover, the temperature effect was more significant than the total strain amplitude influences in cyclic behaviors.
摘要:本文研究了热处理铝基纳米粘土复合材料(AlSi_N_HT6)与活塞式铝合金(AlSi)在应变控制载荷下的循环硬化/软化行为(实验数据)。为此,采用重力法和搅拌铸造法制备了标准样品。进行了不同应变幅值(0.20 ~ 0.45%)和不同温度(25 ~ 300℃)下的低周疲劳试验。结果表明,增强后铝合金的材料性能没有明显变化,但随着温度的升高,材料性能有所下降。结果还表明,温度从25℃升高到200℃,两种材料(AlSi_N_HT6和AlSi)的循环行为都发生了由硬化到软化的变化。温度效应比总应变幅值对循环行为的影响更为显著。
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引用次数: 0
Evaluating ex situ rates of carbon dioxide flux from northern Borneo peat swamp soils 评估北婆罗洲泥炭沼泽土壤的迁地二氧化碳通量率
Pub Date : 2022-01-18 DOI: 10.1017/exp.2022.2
Eliza Low Ying Si, M. Chadwick, T. Smith, Rahayu Sukmaria Sukri
Abstract This study quantified CO2 emissions from tropical peat swamp soils in Brunei Darussalam. At each site, soil was collected from areas of intact and degraded peat and CO2 flux, and total organic content were measured ex situ. Soil organic content (~20–99%) was not significantly different between intact and degraded forest samples. CO2 flux was higher for intact forest samples than degraded forest samples (~1.0 vs. ~0.6 μmol CO2 m−2 s−1, respectively) but did not differ among forest locations. From our laboratory experiments, we estimated a potential emissions of ~10–20 t CO2 ha−1 y−1 which is in the lower range of values reported for other tropical peat swamps. However, our results are likely affected by unmeasured variation in root respiration and the lability of resident carbon. Overall, these findings provide experimental evidence to support that clearance of tropical peat swamp forests can increase CO2 emissions due to faster rates of decomposition.
本研究量化了文莱达鲁萨兰国热带泥炭沼泽土壤的二氧化碳排放。在每个站点,从完整和退化的泥炭和CO2通量区收集土壤,并测量总有机含量。土壤有机质含量(~20 ~ 99%)在完整和退化森林样品间无显著差异。完整森林样品的CO2通量高于退化森林样品(分别为~1.0 μmol CO2 m−2 s−1和~0.6 μmol CO2 m−2 s−1),但不同地点之间没有差异。从我们的实验室实验中,我们估计了~10 - 20t CO2的潜在排放量,这是在其他热带泥炭沼泽报告值的较低范围内。然而,我们的结果可能受到未测量的根呼吸变化和常驻碳不稳定性的影响。总的来说,这些发现提供了实验证据,支持热带泥炭沼泽森林的砍伐可以增加二氧化碳排放,因为分解速度更快。
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引用次数: 0
The robustness of land equivalent ratio as a measure of yield advantage of multi-crop systems over monocultures 土地等效比率作为衡量多作物系统相对于单一作物系统产量优势的稳健性
Pub Date : 2022-01-13 DOI: 10.1017/exp.2021.33
Debal Deb, Sreejata Dutta
Abstract Land equivalent ratio (LER) is a most widely used indicator of yield advantage of multi-crop farms over sole-crop farms, and usually measured using crop biomass yield per unit area. Most often, crop yields are compared between both systems using the same area. In this paper, we demonstrate that although the yield per unit area and the yield per plant are widely different, LER remains invariant. As a corollary, area time equivalent ratio and land use efficiency, derived from LER, also remain unchanged when using the two different measures of crop yields. We recommend that when the estimation of the exact land area is difficult due to complex crop planting designs, yield per plant estimate is much easier and equally valid for estimation of LER and its derivative indices.
土地当量比(Land equivalent ratio, LER)是衡量多作物农场相对单一作物农场产量优势最广泛使用的指标,通常使用单位面积作物生物量来衡量。大多数情况下,在使用相同面积的两种系统之间比较作物产量。在本文中,我们证明了尽管单位面积产量和单株产量差异很大,但LER保持不变。因此,在使用两种不同的作物产量指标时,由LER得出的面积时间当量比和土地利用效率也保持不变。我们建议,当由于复杂的作物种植设计而难以估计准确的土地面积时,单株产量估计要容易得多,并且对LER及其衍生指数的估计同样有效。
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引用次数: 0
A negative charge at position D+5 of Motif A is critical for function of the major facilitator superfamily multidrug/H+antiporter MdtM Motif A的D+5位置的负电荷对主要促进剂超家族多药/H+反转运蛋白MdtM的功能至关重要
Pub Date : 2022-01-13 DOI: 10.1017/exp.2022.1
C. J. Law
Abstract The phenomenon of antimicrobial resistance represents a major public health risk. The activity of integral membrane transporter proteins contributes to antimicrobial resistance in pathogenic bacteria and proton gradient-driven multidrug efflux representatives of the major facilitator superfamily (MFS) of secondary transporters are the dominant antimicrobial efflux proteins in Escherichia coli. In many, but not all, of the characterized MFS multidrug transporters, an aspartic acid residue at position D+5 of the conserved signature Motif A is essential for transport activity. The present work extends those studies to the E. coli MFS multidrug/H+ antiporter MdtM and used a combination of mutagenesis, expression studies, antimicrobial resistance assays, and transport activity measurements to reveal that a negatively charged residue at position D+5 is critical for MdtM transport function.
抗菌素耐药性现象是一项重大的公共卫生风险。整体膜转运蛋白的活性有助于病原菌的耐药,而质子梯度驱动的多药外排是大肠杆菌中主要的抗菌外排蛋白,其主要促进剂超家族(MFS)是二级转运蛋白的代表。在许多(但不是全部)表征的MFS多药转运体中,保守特征Motif A的D+5位置的天冬氨酸残基对转运活性至关重要。目前的工作将这些研究扩展到大肠杆菌MFS多药/H+反转运蛋白MdtM,并结合诱变、表达研究、抗微生物药物耐药性试验和运输活性测量,揭示了D+5位置的带负电荷残基对MdtM的运输功能至关重要。
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引用次数: 0
An assessment of hydrogen sulfide intrusion in the seagrass Halodule wrightii 海草中硫化氢入侵的评价
Pub Date : 2022-01-01 DOI: 10.1017/exp.2022.15
Sebastian Rubiano-Rincon, P. Larkin
Hydrogen sulfide (H2S, “sulfide”) is a naturally occurring component of the marine sediment. Eutrophication of coastal waters, however, can lead to an excess of sulfide production that can prove toxic to seagrasses. We used stable sulfur isotope ratio (δ34S) measurements to assess sulfide intrusion in the seagrass Halodule wrightii, a semi-tropical species found throughout the Gulf of Mexico, Caribbean Sea, and both western and eastern Atlantic coasts. We found a gradient in δ34S values (−5.58 ± 0.54‰+13.58 ± 0.30‰) from roots to leaves, in accordance with prior observations and those from other species. The results may also represent the first values reported for H. wrightii rhizome tissue. The presence of sulfide-derived sulfur in varying proportions (15–55%) among leaf, rhizome, and root tissues suggests H. wrightii is able to assimilate sedimentary H2S into non-toxic forms that constitute a significant portion of the plant’s total sulfur content.
硫化氢(H2S,“硫化物”)是海洋沉积物中自然存在的成分。然而,沿海水域的富营养化可能导致硫化物过量产生,这可能对海草有毒。我们使用稳定的硫同位素比值(δ34S)测量来评估硫化物在海草Halodule wrightii中的入侵,Halodule wrightii是一种遍布墨西哥湾、加勒比海以及大西洋西部和东部海岸的亚热带物种。从根到叶的δ34S值呈−5.58±0.54‰+13.58±0.30‰的梯度,与前人和其他物种的观测结果一致。该结果也可能代表了第一次报道的赖特根组织值。叶片、根茎和根组织中不同比例(15-55%)硫化物衍生的硫的存在表明,wright能够将沉积的H2S吸收成无毒形式,构成植物总硫含量的很大一部分。
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引用次数: 0
Experimental auction confirmation that social desirability bias does not predict willingness to pay for eco-labeled goods 实验拍卖证实,社会期望偏差并不预测愿意支付生态标签的商品
Pub Date : 2021-12-10 DOI: 10.1017/exp.2021.24
Kieran Higgins, A. Longo, George Hutchinson
Abstract It is often assumed that consumers’ willingness to pay (WTP) for eco-labeled products in research settings is not because of a desire for environmental protection, but rather that they are socially compelled to make decisions that reflects favorably on them, limiting the validity of findings. Using a second-price Vickrey experimental auction, this study found higher WTP for an eco-labeled product than a comparable good, but that social desirability bias, measured by the Marlowe–Crowne Social Desirability Scale, was not a significant predictor of WTP. Instead, environmental consciousness, environmental knowledge, education, and available information were stronger predictors of WTP for eco-labeled goods.
人们通常认为,在研究环境中,消费者愿意为生态标签产品付费(WTP)不是因为对环境保护的渴望,而是因为他们在社会上被迫做出有利于他们的决定,从而限制了研究结果的有效性。通过二次价格的Vickrey实验拍卖,本研究发现,生态标签产品的WTP高于同类产品,但由marlow - crown社会可取性量表测量的社会可取性偏差并不是WTP的重要预测因素。相反,环境意识、环境知识、教育和可用信息是生态标签商品WTP的更强预测因子。
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引用次数: 0
Exposure to the anthelmintic dinitroaniline oryzalin causes changes in meiotic prophase morphology and loss of synaptonemal complexes in the nematode Caenorhabditis elegans 在秀丽隐杆线虫中,暴露于二硝基苯胺oryzalin会引起减数分裂前期形态的变化和突触复合物的丧失
Pub Date : 2021-12-10 DOI: 10.1017/exp.2021.19
P. Goldstein
Abstract The anthelmintic dinitroaniline oryzalin interferes with the formation of microtubules and inhibits meiosis and mitosis in nematodes. Exposure to oryzalin resulted in deterioration in morphology of the oocytes and loss of synaptonemal complexes at meiotic prophase I. The nuclear matrix and envelope were poorly formed, and the central rachis was diminished. These results provide the basis for the loss of fecundity after treatment with the oryzalin resulting in control of parasitic nematodes.
二硝基苯胺oryzalin干扰线虫微管的形成,抑制减数分裂和有丝分裂。暴露于oryzalin导致卵母细胞形态恶化,并在减数分裂前期失去突触复合物,核基质和包膜形成不良,中央轴减少。这些结果为用米草甘膦处理后产生的控制寄生线虫的生殖力丧失提供了依据。
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引用次数: 3
Common names decreased in Japan: Further evidence of an increase in individualism 日本常见的名字减少:个人主义增加的进一步证据
Pub Date : 2021-12-10 DOI: 10.1017/exp.2021.27
Yuji Ogihara
Abstract Previous research has demonstrated that unique names increased in Japan, which shows a rise in uniqueness-seeking and individualism. To increase the validity of the prior findings, it is important to confirm the robustness of their results. Therefore, this study examined another indicator of historical changes in names in Japan. Specifically, I investigated whether the rates of common names decreased in Japan between 2004 and 2018. The dataset used in the previous study was analyzed. The results consistently showed that the rates of common names decreased for both boys and girls for the period. These results were consistent with the previous research, which further increases the validity of the finding that Japanese culture became more individualistic.
以往的研究表明,独特的名字在日本有所增加,这表明追求独特和个人主义的兴起。为了提高先前发现的有效性,重要的是要确认他们的结果的稳健性。因此,本研究考察了日本人名历史变化的另一个指标。具体来说,我调查了2004年至2018年期间日本常用名字的使用率是否下降。对之前研究中使用的数据集进行分析。研究结果一致表明,在此期间,男孩和女孩的常用名字比例都有所下降。这些结果与之前的研究一致,这进一步增加了日本文化变得更加个人主义这一发现的有效性。
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引用次数: 8
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Experimental Results
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