S. Kolotilov, V. Melnyk, A. Lytvynenko, S. O. Sotnik, O. Hurzhii
This paper presents a comparison ofspectroscopic properties of aqueous humour in patients with cataract (60 patients, 60 eyes) and combination of cataract and primary open angle glaucoma (POAG, 36 patients, 36 eyes). The samples of aqueous humour were collected during the surgery. Both groups of patients were treated with different types of local drug antihypertensive therapy with different multiplicity and duration of instillation during the preoperative period. The absorption spectrum of aqueous humour were measured in the wavelength range 190-1100 nm. There were two absorption bands with maximums at 202 and 267 nm in the absorption spectrum (2nd maximum of some samples was shifted to larger wavelengths). The mean absorbance of aqueous humour at 202 nm in patients without POAG was significantly lower than in patients with POAG. This difference could be explained with accumulation of metabolites in aqueous humour of patients with POAG, which in turn may be caused by deceleration of aqueous humour flow in patients with POAG. Meanwhile, there were no statistically significant differences of mean absorbance of aqueous humour at 267 nm between the patients of both groups.
{"title":"COMPARISON OF SPECTROSCOPIC PROPERTIES OF INTRAOCULAR FLUID IN PATIENTS WITH CATARACT AND PRIMARY OPEN-ANGLE GLAUCOMA.","authors":"S. Kolotilov, V. Melnyk, A. Lytvynenko, S. O. Sotnik, O. Hurzhii","doi":"10.15407/FZ62.05.062","DOIUrl":"https://doi.org/10.15407/FZ62.05.062","url":null,"abstract":"This paper presents a comparison ofspectroscopic properties of aqueous humour in patients with cataract (60 patients, 60 eyes) and combination of cataract and primary open angle glaucoma (POAG, 36 patients, 36 eyes). The samples of aqueous humour were collected during the surgery. Both groups of patients were treated with different types of local drug antihypertensive therapy with different multiplicity and duration of instillation during the preoperative period. The absorption spectrum of aqueous humour were measured in the wavelength range 190-1100 nm. There were two absorption bands with maximums at 202 and 267 nm in the absorption spectrum (2nd maximum of some samples was shifted to larger wavelengths). The mean absorbance of aqueous humour at 202 nm in patients without POAG was significantly lower than in patients with POAG. This difference could be explained with accumulation of metabolites in aqueous humour of patients with POAG, which in turn may be caused by deceleration of aqueous humour flow in patients with POAG. Meanwhile, there were no statistically significant differences of mean absorbance of aqueous humour at 267 nm between the patients of both groups.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"42 1","pages":"62-68"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74705435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Konopelnyuk, L. Kot, T. Koval, T. D. Khilko, I. Yakubtsova, T. Preobrazhenska, L. Ostapchenko
The content of reduced glutathione (GSH) and activity of glutathione-dependent enzymes: glutathione peroxidase (GP) and glutathione-S-transferase (GT) in blood serum of rats in the diet supplemented of fine powder seeds Trigonella foenum graecum L. on the background of experimental obesity. It was established that the development of obesity in rats was accompanied by violation of homeostasis in the glutathione-dependent system of blood serum. It has been shown that animals on high-calorie diet-along 14 weeks had a decrease (1,2) in GSH concentration, a decline (1,7) in GP activity and the increase (1,7) of GT activity compared with control values. Addition to high-calorie diet 2% fine powder seeds Trigonella foenum graccum L. reduced the body weight gain by 21%, carried positive modulating effect on the content of GSH and GP, GT activity in animals with experimental obesity.
{"title":"STATUS OF GLUTATHIONE-DEPENDENT LINK OF BLOOD ANTIOXIDANT SYSTEM UNDER APPLICATION OF FENUGREEK SEED POWDER IN ANIMALS WITH EXPERIMENTAL OBESITY.","authors":"V. Konopelnyuk, L. Kot, T. Koval, T. D. Khilko, I. Yakubtsova, T. Preobrazhenska, L. Ostapchenko","doi":"10.15407/FZ62.05.069","DOIUrl":"https://doi.org/10.15407/FZ62.05.069","url":null,"abstract":"The content of reduced glutathione (GSH) and activity of glutathione-dependent enzymes: glutathione peroxidase (GP) and glutathione-S-transferase (GT) in blood serum of rats in the diet supplemented of fine powder seeds Trigonella foenum graecum L. on the background of experimental obesity. It was established that the development of obesity in rats was accompanied by violation of homeostasis in the glutathione-dependent system of blood serum. It has been shown that animals on high-calorie diet-along 14 weeks had a decrease (1,2) in GSH concentration, a decline (1,7) in GP activity and the increase (1,7) of GT activity compared with control values. Addition to high-calorie diet 2% fine powder seeds Trigonella foenum graccum L. reduced the body weight gain by 21%, carried positive modulating effect on the content of GSH and GP, GT activity in animals with experimental obesity.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"129 1","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85124356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To investigate the genetical precursors of cataract development the next groups were included: patients suffering from cataract (96) and 96 healthy persons. The determination of γ-crystallin polymorphism (G(-47)-->A) (rs2289917) was provided using PCR method and further analyses of restriction fragment length polymorphism. These allelic variants have the significant different: G/G--35.37%, G/A--53.66%, A/A--10.98%, and G/G--55.06%, G/A--35.96%, A/A--8.99% comparing with the control group (P = 0.03, by χ2-test). While investigating the level of expression of γ-crystallin gene (CRYGB) in platelets, showed that the quantity of mRNA in homozygotes G/G in 3.9 times (P < 0.05) higher than in carriers of A allele (genotype G/A and A/A). This paper shows the significant difference in distribution of CRYGB promoter (G(-47)-->A) genotypes in patients with cataract compared to the control group. Furthermore, here we provide the data concerning its functional meaning: level of mRNA expression of crystallin is different in carriers of various CRYGB promoter (G(-47)-->A) genotypes.
{"title":"[ALLELIC VARIANT FREQUENCY OF PROMOTER (G(-47)-->A) γ-CRYSTALLIN GENE AFFECTS THE LEVEL OF ITS EXPRESSION IN PLATELETS].","authors":"S. Rykov, Y. Y. Byts, S. Goncharov, V. Dosenko","doi":"10.15407/FZ61.04.030","DOIUrl":"https://doi.org/10.15407/FZ61.04.030","url":null,"abstract":"To investigate the genetical precursors of cataract development the next groups were included: patients suffering from cataract (96) and 96 healthy persons. The determination of γ-crystallin polymorphism (G(-47)-->A) (rs2289917) was provided using PCR method and further analyses of restriction fragment length polymorphism. These allelic variants have the significant different: G/G--35.37%, G/A--53.66%, A/A--10.98%, and G/G--55.06%, G/A--35.96%, A/A--8.99% comparing with the control group (P = 0.03, by χ2-test). While investigating the level of expression of γ-crystallin gene (CRYGB) in platelets, showed that the quantity of mRNA in homozygotes G/G in 3.9 times (P < 0.05) higher than in carriers of A allele (genotype G/A and A/A). This paper shows the significant difference in distribution of CRYGB promoter (G(-47)-->A) genotypes in patients with cataract compared to the control group. Furthermore, here we provide the data concerning its functional meaning: level of mRNA expression of crystallin is different in carriers of various CRYGB promoter (G(-47)-->A) genotypes.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"183 1","pages":"30-4"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74647921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Ishchuk, O. Savchuk, Y. Raetska, V. Vereschaka, L. Ostapchenko
The main result of esophagus burn is the formation of scars, that caused by excessive synthesis of collagen and changes the balance of metalloproteinases and their tissue inhibitors. It was studied the activity of proteolytic enzymes, participation of MMP (metalloproteinase) and their tissue inhibitors (TIMP) in alkali burns of the esophagus 1st and 2nd degrees. We have shown a significant increase of TIMP level in homogenate after alkali burns of the esophagus (an average of 31-56% depend on of burn degree). We observed a reduced activity of serine proteinase after 1st degree burns on 15th, 21st day 35 and 18% respectively, after burns 2nd degree on 15th, 21st day 54 and 50%. The decrease of activity MMP after 1st degree burns on 15th and 21st day 30, 19%, respectively, in conditions of chemical burns 2nd degree on 15th and 21st day 30, 37%. These data may indicate the development of scarring after burn simulation of 2nd degree. Further investigation of the MMP and TIMP in the process of wound healing can be useful in creating effective approaches to prevent formation of post scarring of the esophagus.
{"title":"[FUNCTIONING PROTEASES IN THE ESOPHAGUS MUCOSA AFTER CHEMICAL BURNS].","authors":"T. Ishchuk, O. Savchuk, Y. Raetska, V. Vereschaka, L. Ostapchenko","doi":"10.15407/FZ61.05.071","DOIUrl":"https://doi.org/10.15407/FZ61.05.071","url":null,"abstract":"The main result of esophagus burn is the formation of scars, that caused by excessive synthesis of collagen and changes the balance of metalloproteinases and their tissue inhibitors. It was studied the activity of proteolytic enzymes, participation of MMP (metalloproteinase) and their tissue inhibitors (TIMP) in alkali burns of the esophagus 1st and 2nd degrees. We have shown a significant increase of TIMP level in homogenate after alkali burns of the esophagus (an average of 31-56% depend on of burn degree). We observed a reduced activity of serine proteinase after 1st degree burns on 15th, 21st day 35 and 18% respectively, after burns 2nd degree on 15th, 21st day 54 and 50%. The decrease of activity MMP after 1st degree burns on 15th and 21st day 30, 19%, respectively, in conditions of chemical burns 2nd degree on 15th and 21st day 30, 37%. These data may indicate the development of scarring after burn simulation of 2nd degree. Further investigation of the MMP and TIMP in the process of wound healing can be useful in creating effective approaches to prevent formation of post scarring of the esophagus.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"17 1","pages":"71-7"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78191770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of this study was to show the effect of H₂S donor, NaHS on the endothelium-dependent vasorelaxation, free radical state and cNOS uncoupling in old rats. In the aorta of old rats a combined oxidative and nitrosative stress develops that leads to cNOS uncoupling and decreased constitutive synthesis of the NO. That biochemical changes correlate with lowering of the endothelium-dependent relaxation of aortic smooth muscles (7.5 ± 1.4%, compared with 64.9 ± 3.5% in adults). It was found that, due to the combined inhibition of oxidative and nitrosative stress, NaHS restores constitutive de novo synthesis of NO by restoring cNOS coupling. Additionally, NaHS improves endothelium-dependent vasorelaxation by increasing (by 6.5 times) Ach-induced relaxation of aortic smooth muscles.
{"title":"[HYDROGEN SULFIDE DONOR, NAHS, RECOVERS CONSTITUTIVE NO SYNTHESIS AND ENDOTHELIUM-DEPENDENT RELAXATION OF ISOLATED AORTA IN OLD RATS].","authors":"K. Drachuk, A. Kotsjuruba, V. Sagach","doi":"10.15407/FZ61.06.003","DOIUrl":"https://doi.org/10.15407/FZ61.06.003","url":null,"abstract":"The objective of this study was to show the effect of H₂S donor, NaHS on the endothelium-dependent vasorelaxation, free radical state and cNOS uncoupling in old rats. In the aorta of old rats a combined oxidative and nitrosative stress develops that leads to cNOS uncoupling and decreased constitutive synthesis of the NO. That biochemical changes correlate with lowering of the endothelium-dependent relaxation of aortic smooth muscles (7.5 ± 1.4%, compared with 64.9 ± 3.5% in adults). It was found that, due to the combined inhibition of oxidative and nitrosative stress, NaHS restores constitutive de novo synthesis of NO by restoring cNOS coupling. Additionally, NaHS improves endothelium-dependent vasorelaxation by increasing (by 6.5 times) Ach-induced relaxation of aortic smooth muscles.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"10 1","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81954254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.1615/INTJPHYSPATHOPHYS.V7.I4.80
G. Maleeva, P. Bregestovski
Glycine receptor is the anion-selective channel, providing fast synaptic transmission in the central nervous system of vertebrates. Together with the nicotinic acetylcholine, GABA and serotonin (5-HT3R) receptors, it belongs to the superfamily of pentameric cys-loop receptors. In this review we briefly describe main functions of these transmembrane proteins, their distribution and molecular architecture. Special attention is paid to recent studies on the molecular physiology of these receptors, as well as on presenting of molecular domains responsible for their dysfunction.
{"title":"[GLYCINE RECEPTOR: MOLECULAR ORGANIZATION AND PATHOLOGY].","authors":"G. Maleeva, P. Bregestovski","doi":"10.1615/INTJPHYSPATHOPHYS.V7.I4.80","DOIUrl":"https://doi.org/10.1615/INTJPHYSPATHOPHYS.V7.I4.80","url":null,"abstract":"Glycine receptor is the anion-selective channel, providing fast synaptic transmission in the central nervous system of vertebrates. Together with the nicotinic acetylcholine, GABA and serotonin (5-HT3R) receptors, it belongs to the superfamily of pentameric cys-loop receptors. In this review we briefly describe main functions of these transmembrane proteins, their distribution and molecular architecture. Special attention is paid to recent studies on the molecular physiology of these receptors, as well as on presenting of molecular domains responsible for their dysfunction.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"1 1","pages":"107-17"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90509152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We studied the expression of AhR and ATG16L1 protein in experimental oxazolone-induced colitis in rats and anti-inflammatory action of recombinant antagonist of IL-1 receptors (ARIL-1) and simvastatin. The immunopositive cells were determined using an indirect immunofluorescence technique with using a monoclonal rat antibody. It has been established that development of colitis was accompanied by an increase of total number of ATG16L1-lymphocytes (by 30%, P < 0.05) in lymphoid structures of the colon. However the amount of AhR(+)-lymphocytes has not changed. At the same time has increased the concentration of ATG16L1 protein (by 4-11%, P < 0.05) in immunopositive cells. Administration of simvastatin and ARIL-1 during the development of experimental pathology was accompanied by decrease of total number of AhR(+) (by 24-38%, P < 0.05) and ATG16L1(+)-lymphocytes (by 43% - 2 fold, P < 0.05) in the colon.
{"title":"Expression of aryl hydrocarbon receptor and ATG16L1 protein in experimental oxazolone-induced colitis in rats.","authors":"Zherebiatiev As, Kamyshny Am","doi":"10.15407/FZ61.05.057","DOIUrl":"https://doi.org/10.15407/FZ61.05.057","url":null,"abstract":"We studied the expression of AhR and ATG16L1 protein in experimental oxazolone-induced colitis in rats and anti-inflammatory action of recombinant antagonist of IL-1 receptors (ARIL-1) and simvastatin. The immunopositive cells were determined using an indirect immunofluorescence technique with using a monoclonal rat antibody. It has been established that development of colitis was accompanied by an increase of total number of ATG16L1-lymphocytes (by 30%, P < 0.05) in lymphoid structures of the colon. However the amount of AhR(+)-lymphocytes has not changed. At the same time has increased the concentration of ATG16L1 protein (by 4-11%, P < 0.05) in immunopositive cells. Administration of simvastatin and ARIL-1 during the development of experimental pathology was accompanied by decrease of total number of AhR(+) (by 24-38%, P < 0.05) and ATG16L1(+)-lymphocytes (by 43% - 2 fold, P < 0.05) in the colon.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"23 1","pages":"57-64"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74749311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kotsuruba, R. Sharipov, B. S. Kopyak, V. Sagach
We investigated the resistance of erythrocytes from rat brain venous blood to acid hemolysis in the dynamics of brain ischemic period (15, 30, 45 and 60 min), as well as in the early (5 min) and distant (24h) period of brain reperfusion. Brain ischemia-reperfusion was made in rats that received ecdysterone (standartized extract of Serratula coronata) within 18 days (per os, 1 mg/kg). Analysis of the kinetic curves of acid hemolysis showed a pronounced (60 times, from 1.45 to 85.85% at 60 min of brain ischemia and at 5 min of brain reperfusion, respectively) increase of unstable erythrocytes that hemolyzed easily (< 2.5 min). In the preconditioned rats, this increase was only 8-fold. During the period of brain ischemia, with a maximum at 15th minute, in the venous blood from brain the diene conjugates (DK) pools increased from 2.40 to 9.48 ng/mg protein and LTC4 pools increased from 1.49 to 5.98 pmol/mg protein. Even more pools of DC and LTC4 were increased at 5th min of brain reperfusion. In animals received ecdysterone, during ischemia and early reperfusion period, both pools of DC and LTC4 in venous blood were lower than that in the controls. The latter implies a possible antiradical mechanism of the protective effect of ecdysterone.
{"title":"[BRAIN FOCAL ISCHEMIA-REPERFUSION CAUSES A DECREASED RESISTANCE OF ERYTHROCYTES FROM VENOUS BLOOD TO ACID HEMOLYSIS, WHICH IS PREVENTED BY ECDYSTERONE].","authors":"A. Kotsuruba, R. Sharipov, B. S. Kopyak, V. Sagach","doi":"10.15407/FZ61.05.003","DOIUrl":"https://doi.org/10.15407/FZ61.05.003","url":null,"abstract":"We investigated the resistance of erythrocytes from rat brain venous blood to acid hemolysis in the dynamics of brain ischemic period (15, 30, 45 and 60 min), as well as in the early (5 min) and distant (24h) period of brain reperfusion. Brain ischemia-reperfusion was made in rats that received ecdysterone (standartized extract of Serratula coronata) within 18 days (per os, 1 mg/kg). Analysis of the kinetic curves of acid hemolysis showed a pronounced (60 times, from 1.45 to 85.85% at 60 min of brain ischemia and at 5 min of brain reperfusion, respectively) increase of unstable erythrocytes that hemolyzed easily (< 2.5 min). In the preconditioned rats, this increase was only 8-fold. During the period of brain ischemia, with a maximum at 15th minute, in the venous blood from brain the diene conjugates (DK) pools increased from 2.40 to 9.48 ng/mg protein and LTC4 pools increased from 1.49 to 5.98 pmol/mg protein. Even more pools of DC and LTC4 were increased at 5th min of brain reperfusion. In animals received ecdysterone, during ischemia and early reperfusion period, both pools of DC and LTC4 in venous blood were lower than that in the controls. The latter implies a possible antiradical mechanism of the protective effect of ecdysterone.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"22 1","pages":"3-10"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82378256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Shypshyna, N. Veselovsky, N. Myasoedov, S. Shram, S. Fedulova
The influence of long-term culturing (12 days in vitro) of dorsal root ganglion (DRG) and dorsal horn (DH) neurons with peptide Semax on the level of synaptic activity at co-cultures, as well as short-term plasticity in sensory synapses were studied. It has been shown that neuronal culturing with peptide at concentrations of 10 and 100 µM led to increasing the frequency of spontaneous glutamatergic postsynaptic currents in DH neurons to 71.7 ± 1.8% and 93.9 ± 3.1% (n = 6; P < 0.001); Semax has a not significant effect on the amplitude and frequency of miniature glutamatergic currents, but causes an increase of the amplitudes of spontaneous postsynaptic currents, as well as elevates the quantum content. The data show the increase of multivesicular glutamate release efficiency in neural networks of co-cultures following incubation with the peptide. Also Semax (10 and 100 µM) induces changes of the basic parameters of short-term plasticity in sensory synapses: (1) increasing the paired-pulse ratio from 0.53 ± 0.028 (n = 8) to 0.91 ± 0.072 (n = 6, P < 0.01) and 0.95 ± 0.026 (n = 7; P < 0.001); (2) reducing the ratio of the coefficients of variation (CV2/ CV1) from 1.49 ± 0.11 (n = 8) to 1.02 ± 0.09 (n = 6; P < 0.05) and 1.11 ± 0.13 (n = 7; P < 0.0) respectively. The results indicate a stimulating effect of Semax on the activity of glutamatergic synapses in neural networks of co-cultures, as well as the ability of the peptide to effectively modulate the short-term plasticity in sensory synapses.
{"title":"[EFFECT OF PEPTIDE SEMAX ON SYNAPTIC ACTIVITY AND SHORT-TERM PLASTICITY OF GLUTAMATERGIC SYNAPSES OF CO-CULTURED DORSAL ROOT GANGLION AND DORSAL HORN NEURONS].","authors":"M. Shypshyna, N. Veselovsky, N. Myasoedov, S. Shram, S. Fedulova","doi":"10.15407/FZ61.04.048","DOIUrl":"https://doi.org/10.15407/FZ61.04.048","url":null,"abstract":"The influence of long-term culturing (12 days in vitro) of dorsal root ganglion (DRG) and dorsal horn (DH) neurons with peptide Semax on the level of synaptic activity at co-cultures, as well as short-term plasticity in sensory synapses were studied. It has been shown that neuronal culturing with peptide at concentrations of 10 and 100 µM led to increasing the frequency of spontaneous glutamatergic postsynaptic currents in DH neurons to 71.7 ± 1.8% and 93.9 ± 3.1% (n = 6; P < 0.001); Semax has a not significant effect on the amplitude and frequency of miniature glutamatergic currents, but causes an increase of the amplitudes of spontaneous postsynaptic currents, as well as elevates the quantum content. The data show the increase of multivesicular glutamate release efficiency in neural networks of co-cultures following incubation with the peptide. Also Semax (10 and 100 µM) induces changes of the basic parameters of short-term plasticity in sensory synapses: (1) increasing the paired-pulse ratio from 0.53 ± 0.028 (n = 8) to 0.91 ± 0.072 (n = 6, P < 0.01) and 0.95 ± 0.026 (n = 7; P < 0.001); (2) reducing the ratio of the coefficients of variation (CV2/ CV1) from 1.49 ± 0.11 (n = 8) to 1.02 ± 0.09 (n = 6; P < 0.05) and 1.11 ± 0.13 (n = 7; P < 0.0) respectively. The results indicate a stimulating effect of Semax on the activity of glutamatergic synapses in neural networks of co-cultures, as well as the ability of the peptide to effectively modulate the short-term plasticity in sensory synapses.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"24 1","pages":"48-55"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86633896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Morphofunctional state of the thyroid gland (TG) of young rats after exposure of exogenous melatonin in the spring and autumn periods was investigated. Exogenous melatonin (Unipharm Inc., USA) was administered to experimental group of animals daily at a dose 5 mg/kg. The duration of the experiment was 28 days. It was shown an increase of the cross-section area of follicles by 31% and colloid by 30% (in spring), reduction of the area of follicle epithelium by 12% (in autumn), an increase in the follicle internal diameter and a decline in the thyroid epithelium height by 12% (in autumn) in TG of experimental groups rats. Also it was shown a decline of follicle-colloid index and growth of colloid accumulation index, reduction amount of interfollicular islets. The introduction of melatonin in the spring period brought down the amount of connecting tissue elements. Melatonin introduction in the autumn period reduced the thickness of connective tissue trabecules in TG. The introduction of melatonin in the spring period resulted in more substantial changes in the structure of TG, as compared to an autumn experiment. Thus, melatonin administered both in the spring and in the autumn periods reduces the functional activity and physiological regeneration of TG.
{"title":"[MORPHOFUNCTIONAL STATE OF THE THYROID GLAND AFTER EXPOSURE TO MELATONIN].","authors":"R. Yanko","doi":"10.15407/FZ61.05.046","DOIUrl":"https://doi.org/10.15407/FZ61.05.046","url":null,"abstract":"Morphofunctional state of the thyroid gland (TG) of young rats after exposure of exogenous melatonin in the spring and autumn periods was investigated. Exogenous melatonin (Unipharm Inc., USA) was administered to experimental group of animals daily at a dose 5 mg/kg. The duration of the experiment was 28 days. It was shown an increase of the cross-section area of follicles by 31% and colloid by 30% (in spring), reduction of the area of follicle epithelium by 12% (in autumn), an increase in the follicle internal diameter and a decline in the thyroid epithelium height by 12% (in autumn) in TG of experimental groups rats. Also it was shown a decline of follicle-colloid index and growth of colloid accumulation index, reduction amount of interfollicular islets. The introduction of melatonin in the spring period brought down the amount of connecting tissue elements. Melatonin introduction in the autumn period reduced the thickness of connective tissue trabecules in TG. The introduction of melatonin in the spring period resulted in more substantial changes in the structure of TG, as compared to an autumn experiment. Thus, melatonin administered both in the spring and in the autumn periods reduces the functional activity and physiological regeneration of TG.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"24 1","pages":"46-51"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85620905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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