We explored the changes in reduced glutathione in pathogenesis of severe and combined trauma. Under conditions of our experiments both in blood plasma and tissue of liver, kidneys and heart the concentration of reduced glutathione is decreased. Comparison of the changes ofglutathione that occur in animals of the first experimental group (only severe group) with the same in animals of the second and the third groups, caused by the influence of extra mechanical defect and skin burn, showed that in the last group the disorders of functional status system of antioxidant protection occur more often than in only severely injured animals. The largest significant decrease (34%) in the content of GSH on the 3rd day after injury we found in liver of seriously injured burned animals. In animals of the first and second groups the content of GSH decreased by 27 and 33.7%, respectively.
{"title":"[Changes in the content of reduced glutathione in severe injuries and multiple trauma].","authors":"S. Pidruchna","doi":"10.15407/FZ59.02.092","DOIUrl":"https://doi.org/10.15407/FZ59.02.092","url":null,"abstract":"We explored the changes in reduced glutathione in pathogenesis of severe and combined trauma. Under conditions of our experiments both in blood plasma and tissue of liver, kidneys and heart the concentration of reduced glutathione is decreased. Comparison of the changes ofglutathione that occur in animals of the first experimental group (only severe group) with the same in animals of the second and the third groups, caused by the influence of extra mechanical defect and skin burn, showed that in the last group the disorders of functional status system of antioxidant protection occur more often than in only severely injured animals. The largest significant decrease (34%) in the content of GSH on the 3rd day after injury we found in liver of seriously injured burned animals. In animals of the first and second groups the content of GSH decreased by 27 and 33.7%, respectively.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"8 1","pages":"92-5"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90041050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. B. Strutyns'kyĭ, A. Kotsiuruba, R. A. Rovenets, N. A. Strutyns'ka, Iu L Iagupols'kyĭ, V. Sagach, O. Moibenko
In experiments on the anaesthetized dogs with modeling of experimental ischemia (90 min) and reperfusion (180 min) of myocardium it was investigated changes of biochemical processes in arterial blood at intragastric introduction of medicinal form (tablets) of flocalin (the fluorine-containing opener of ATP-sensitive potassium channels) in a dose 2,2 mg/kg. The data analysis allowed to define a few possible mechanisms of cardioprotective action offlocalin, which prevented the opening of a mitochondrial permeability transition pore (MPTP) and inhibition of apoptosis induced by it. They consist, from one side, in activating of the constitutive de novo biosynthesis of nitric oxide by cNOS, from other side, in suppression of inducible nitric oxide de novo synthesis by iNOS in such way to prevent the formation of toxic peroxynitrite by co-operation of surplus nitric oxide with superoxide anion, thereby limits the generation of toxic active forms of nitrogen (*NO2) and oxygen (*OH). The first effect of flocalin takes place due to limitation the degradation of L-arginine by arginase which keeps substrat for cNOS, second--due to the inhibition of superoxide generation, in particular, by xanthine oxidase (marker uric acid), lipoxigenase (marker LTC4) and cyclooxygenase (marker TxB2). Because LTC4 have coronaroconstrictory, arrhythmogenic and chemoattractory properties in the conditions of myocardial ischemia, inhibition of its production both with superoxide generation (markers H2O2 and diene conjugates) may be the another mechanisms of flocalin's cardioprotection. Powerful antiischemic action of flocalin (marker nitrite anion) as the mechanisms of cardioprotection is possible as well as inhibition of ATP and GTP degradation (marker hypoxanthine+xanthine+inosine levels in the blood) and, possibly, stimulation ofhaem degradation by haem oxygenase (markers total bilirubin and Fe in the blood). Diminishing content of free arachidonic acid in arterial blood can testify inhibition of cellular membranes phospholipides degradation by phospholipase A2 as a result of flocalin cardioprotection.
{"title":"[Biochemical mechanisms of the cardioprotective effect of the K(ATP) channels opener flocalin (medicinal form) in ischemia-reperfusion of myocardium].","authors":"R. B. Strutyns'kyĭ, A. Kotsiuruba, R. A. Rovenets, N. A. Strutyns'ka, Iu L Iagupols'kyĭ, V. Sagach, O. Moibenko","doi":"10.15407/FZ59.04.016","DOIUrl":"https://doi.org/10.15407/FZ59.04.016","url":null,"abstract":"In experiments on the anaesthetized dogs with modeling of experimental ischemia (90 min) and reperfusion (180 min) of myocardium it was investigated changes of biochemical processes in arterial blood at intragastric introduction of medicinal form (tablets) of flocalin (the fluorine-containing opener of ATP-sensitive potassium channels) in a dose 2,2 mg/kg. The data analysis allowed to define a few possible mechanisms of cardioprotective action offlocalin, which prevented the opening of a mitochondrial permeability transition pore (MPTP) and inhibition of apoptosis induced by it. They consist, from one side, in activating of the constitutive de novo biosynthesis of nitric oxide by cNOS, from other side, in suppression of inducible nitric oxide de novo synthesis by iNOS in such way to prevent the formation of toxic peroxynitrite by co-operation of surplus nitric oxide with superoxide anion, thereby limits the generation of toxic active forms of nitrogen (*NO2) and oxygen (*OH). The first effect of flocalin takes place due to limitation the degradation of L-arginine by arginase which keeps substrat for cNOS, second--due to the inhibition of superoxide generation, in particular, by xanthine oxidase (marker uric acid), lipoxigenase (marker LTC4) and cyclooxygenase (marker TxB2). Because LTC4 have coronaroconstrictory, arrhythmogenic and chemoattractory properties in the conditions of myocardial ischemia, inhibition of its production both with superoxide generation (markers H2O2 and diene conjugates) may be the another mechanisms of flocalin's cardioprotection. Powerful antiischemic action of flocalin (marker nitrite anion) as the mechanisms of cardioprotection is possible as well as inhibition of ATP and GTP degradation (marker hypoxanthine+xanthine+inosine levels in the blood) and, possibly, stimulation ofhaem degradation by haem oxygenase (markers total bilirubin and Fe in the blood). Diminishing content of free arachidonic acid in arterial blood can testify inhibition of cellular membranes phospholipides degradation by phospholipase A2 as a result of flocalin cardioprotection.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"6 1","pages":"16-27"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84140095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article presents results of research of the content of glycoproteins and their specific carbohydrate components, total protein, hemoglobin, erythrocyte, leucocytes molecules of average mass and circulating immune complexes in the blood of female rats under conditions feeding of conventional and transgenic soybeans. Also the reproductive function and mass coefficient of fetus of the studied animals was analyzed. The studies were conducted in three groups of female rats aged 3 months. Animals of I group (control) were kept on a balanced diet during the study period. Animals of groups II and III (research) received a diet according to the scheme of the control group with the addition of 30% from the nutritional value diet native or transgenic soybean, respectively. We found that soy feeding had no significant effect on hematological data in the second and third experimental groups versus control. However, addition to the diet of soybeans, including genetically modified, has a significant impact on the content of glycoproteins and their specific carbohydrate components, female's fertility and less pronounced impact on the data of functional status of their immune system. A generalized analysis of the results of research leads to the conclusion that there is no definite negative or positive impact of GM soy components on their physiological state compared with animals fed native soybeans.
{"title":"[The impact of components of conventional and genetically modified soybeans on parameters of the immune and reproductive systems in female rats].","authors":"O. P. Dolaĭchuk, R. Fedoruk, I. Koval'chuk","doi":"10.15407/FZ59.02.065","DOIUrl":"https://doi.org/10.15407/FZ59.02.065","url":null,"abstract":"The article presents results of research of the content of glycoproteins and their specific carbohydrate components, total protein, hemoglobin, erythrocyte, leucocytes molecules of average mass and circulating immune complexes in the blood of female rats under conditions feeding of conventional and transgenic soybeans. Also the reproductive function and mass coefficient of fetus of the studied animals was analyzed. The studies were conducted in three groups of female rats aged 3 months. Animals of I group (control) were kept on a balanced diet during the study period. Animals of groups II and III (research) received a diet according to the scheme of the control group with the addition of 30% from the nutritional value diet native or transgenic soybean, respectively. We found that soy feeding had no significant effect on hematological data in the second and third experimental groups versus control. However, addition to the diet of soybeans, including genetically modified, has a significant impact on the content of glycoproteins and their specific carbohydrate components, female's fertility and less pronounced impact on the data of functional status of their immune system. A generalized analysis of the results of research leads to the conclusion that there is no definite negative or positive impact of GM soy components on their physiological state compared with animals fed native soybeans.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"19 1","pages":"65-70"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87867308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. V. Dolga, N. K. Pogoriela, O. S. Bohorad-Kobel's'ka, N. Zholobak, S. Zanoza, S. A. Liakhov, I. S. Mahura
The early changes of electrophoretic mobility (EPM) of murine T lymphocytes induced by structural analogues of amixine-dihydrochloryde 4,4'-bis-[2(diethylamino)ethoxy]diphenyl (compound 1) and dihydrochloryde 2-methoxycarbonil-4,4'-bis-[2(diethylamino)ethoxy]diphenyl (compound 2) were studied by electrophoresis technique. During the interval 0-2 hours all compounds increased the absolute values of EPM in comparison with control. These changes were of the same kind--distinctions were quantitative. Amixine and compound 1 during the interval 2-4 hours additionally increased the EPM. The compound 2, on the contrary, decreased the EPM. It was shown that the opposite effects of the aforementioned compounds were caused by the fact that amixine and compound 1 induce, and compound 2 does not induce IFN production in T lymphocytes in vitro. The results of our experiments are important for understanding of the mechanisms of immunomodulating effect of amixine and its structural analogues.
{"title":"[Effects of diphenyl derivatives on electrophoretic mobility of murine T lymphocytes].","authors":"O. V. Dolga, N. K. Pogoriela, O. S. Bohorad-Kobel's'ka, N. Zholobak, S. Zanoza, S. A. Liakhov, I. S. Mahura","doi":"10.15407/FZ59.03.031","DOIUrl":"https://doi.org/10.15407/FZ59.03.031","url":null,"abstract":"The early changes of electrophoretic mobility (EPM) of murine T lymphocytes induced by structural analogues of amixine-dihydrochloryde 4,4'-bis-[2(diethylamino)ethoxy]diphenyl (compound 1) and dihydrochloryde 2-methoxycarbonil-4,4'-bis-[2(diethylamino)ethoxy]diphenyl (compound 2) were studied by electrophoresis technique. During the interval 0-2 hours all compounds increased the absolute values of EPM in comparison with control. These changes were of the same kind--distinctions were quantitative. Amixine and compound 1 during the interval 2-4 hours additionally increased the EPM. The compound 2, on the contrary, decreased the EPM. It was shown that the opposite effects of the aforementioned compounds were caused by the fact that amixine and compound 1 induce, and compound 2 does not induce IFN production in T lymphocytes in vitro. The results of our experiments are important for understanding of the mechanisms of immunomodulating effect of amixine and its structural analogues.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"1 1","pages":"31-8"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79130934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Harmanchuk, O. Makarenko, N. M. Khranovs'ka, T. V. Nikolaienko, V. V. Nikulina, Kh D Nepyĭvoda, L. I. Ostapchenko, S. Morozov, M. S. Kositsyn
The effect of mitokorrectine (complex native oligopeptides isolated from neonatal pig brain) on endothelial cells in culture was investigated. It was revealed that the drug concentration-dependently induces angiogenesis in vitro. Mitogen effect of Mitokorrectine was shown by MTT-test and routine cell count in concentration diapason (0.1-1 mg/ml) which means an increased number of cells by 25 +/- 5% and cell subpopulation of proliferative pool (G2/M+S) 1,8 times in concentration diapason mitokorrectine (0.01-0.05 mg/ml) in comparison with control. In 3-D culture and in stationary phase we detected induction of differentiation of endothelial cells, a decrease the level of NO production and enhancement of glucose metabolism and stimulation of formation of capillary-like tubes.
{"title":"[Mitokorrektine stimulates angiogenesis in vitro].","authors":"L. Harmanchuk, O. Makarenko, N. M. Khranovs'ka, T. V. Nikolaienko, V. V. Nikulina, Kh D Nepyĭvoda, L. I. Ostapchenko, S. Morozov, M. S. Kositsyn","doi":"10.15407/FZ59.02.052","DOIUrl":"https://doi.org/10.15407/FZ59.02.052","url":null,"abstract":"The effect of mitokorrectine (complex native oligopeptides isolated from neonatal pig brain) on endothelial cells in culture was investigated. It was revealed that the drug concentration-dependently induces angiogenesis in vitro. Mitogen effect of Mitokorrectine was shown by MTT-test and routine cell count in concentration diapason (0.1-1 mg/ml) which means an increased number of cells by 25 +/- 5% and cell subpopulation of proliferative pool (G2/M+S) 1,8 times in concentration diapason mitokorrectine (0.01-0.05 mg/ml) in comparison with control. In 3-D culture and in stationary phase we detected induction of differentiation of endothelial cells, a decrease the level of NO production and enhancement of glucose metabolism and stimulation of formation of capillary-like tubes.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"13 1","pages":"52-8"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82855711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Voitenko, V. V. Konopel'niuk, O. Savchuk, L. Ostapchenko
We investigated the changes in key parameters of carbohydrate and lipid metabolism, which correspond to the clinical picture that accompanies the development of prediabetic condition on the background of chronic alcohol intoxication. From the analysis of glycemic curves obtained during the insulin-glucose test, a speed of glucose uptake by peripheral tissues increased at the 1st day (1.5 fold) and the third day (1.3 fold) of administration of alcohol solution. At the later periods, at 7 and 11 days of ethanol administration, a decreased rate of glucose uptake in animals with chronic alcohol intoxication was detected. We also detected an increased content of serotonin in the blood serum and a decreased (1.2 fold) serotonin content in rat brain during the whole period of development of chronic alcohol intoxication.
{"title":"[Development of a prediabetic state under chronic alcohol intoxication].","authors":"V. Voitenko, V. V. Konopel'niuk, O. Savchuk, L. Ostapchenko","doi":"10.15407/FZ59.04.033","DOIUrl":"https://doi.org/10.15407/FZ59.04.033","url":null,"abstract":"We investigated the changes in key parameters of carbohydrate and lipid metabolism, which correspond to the clinical picture that accompanies the development of prediabetic condition on the background of chronic alcohol intoxication. From the analysis of glycemic curves obtained during the insulin-glucose test, a speed of glucose uptake by peripheral tissues increased at the 1st day (1.5 fold) and the third day (1.3 fold) of administration of alcohol solution. At the later periods, at 7 and 11 days of ethanol administration, a decreased rate of glucose uptake in animals with chronic alcohol intoxication was detected. We also detected an increased content of serotonin in the blood serum and a decreased (1.2 fold) serotonin content in rat brain during the whole period of development of chronic alcohol intoxication.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"21 1","pages":"33-9"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78109650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kondro, T. Halenova, M. Kuznietsova, O. Savchuk
Nowadays the problem of insulin resistance, which has close cause-effect relations with obesity, diabetes mellitus type 2, metabolic syndrome, etc., is of urgent importance in medicine. We have revealed bidirectional changes of the IR content in crude membrane and cytosol of the adipose tissue cells in rats under conditions of the long-term high-energy diet. It is possible that reduction of the IR content in the adipose tissue cells has been predetermined by the disruption of lipid bilayer of adipocytes as a result of peroxidation processes activation. Increase in the IR content in the cytosol of cells of this tissue may indicate the activation of synthesis of this protein; however, it is possible that the IR translocation process disorder occurs due to the damage of plasma membrane, preventing the transfer of newly synthesized molecules of the receptor to the membrane and causing their accumulation in cytosol. The obtained results show that the tissues react to the long-term consumption of high-energy food in different ways. Thus, the content of insulin receptors in the plasma membrane of the muscle tissue cells increases, and, on the contrary, it decreases in adipose tissue cells. Such results may indicate that IR development at the late period of the experiment is likely the result of the adipose tissue cells disfunction. The obtained data may be of high significance in understanding the mechanism of the IR development under conditions of the long-term consumption of the high-energy food.
{"title":"[Insulin receptor expression in subcellular fractions of muscular and adipose tissue as the factor of the tissue insulin resistance development in rats under conditions of the high-energy diet].","authors":"M. Kondro, T. Halenova, M. Kuznietsova, O. Savchuk","doi":"10.15407/FZ59.02.059","DOIUrl":"https://doi.org/10.15407/FZ59.02.059","url":null,"abstract":"Nowadays the problem of insulin resistance, which has close cause-effect relations with obesity, diabetes mellitus type 2, metabolic syndrome, etc., is of urgent importance in medicine. We have revealed bidirectional changes of the IR content in crude membrane and cytosol of the adipose tissue cells in rats under conditions of the long-term high-energy diet. It is possible that reduction of the IR content in the adipose tissue cells has been predetermined by the disruption of lipid bilayer of adipocytes as a result of peroxidation processes activation. Increase in the IR content in the cytosol of cells of this tissue may indicate the activation of synthesis of this protein; however, it is possible that the IR translocation process disorder occurs due to the damage of plasma membrane, preventing the transfer of newly synthesized molecules of the receptor to the membrane and causing their accumulation in cytosol. The obtained results show that the tissues react to the long-term consumption of high-energy food in different ways. Thus, the content of insulin receptors in the plasma membrane of the muscle tissue cells increases, and, on the contrary, it decreases in adipose tissue cells. Such results may indicate that IR development at the late period of the experiment is likely the result of the adipose tissue cells disfunction. The obtained data may be of high significance in understanding the mechanism of the IR development under conditions of the long-term consumption of the high-energy food.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"56 1","pages":"59-64"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85614018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Kharchuk, O. Andrukhova, V. K. Rybal'chenko, O. Andrukhov
The features of the impact of the maleimide derivative 1-(4-Cl-benzyl)-3-chloro-4-(CF3-fenilamino)-1H-pyrrole-2,5-dione (MI-1) on the viability and apoptosis-induced cell death of renal proximal and distal tubular epithelial cells and the amount of total and phosphorylated ERK1/2 were studied in order to establish possible mechanisms of nephrotoxicity induced by of MI-1. The viability and apoptosis of renal epithelial tubular cells after incubation with MI-1 were perfomed by 3,4,5-dymetyltiazol-2-yl-2,5-diphenyl-tetrazolium bromide (MTT)-test and by flow cytometry after staining with specific antibodies to annexin V, respectively. The amount of ERK 1/2 was determined by Western blotting. The data indicate that MI-1 was more toxic with respect to the epithelial cells of distal than proximal tubule cells. The apoptosis-induced cell death pathway is involved in the mechanisms of MI-1 cytotoxicity. One of the possible mechanisms of MI-1 nephrotoxicity is increase in phosphorylation of ERK1/2 in the distal tubules. At the same time the increase amount of total ERK1/2 in proximal tubules under the influence of MI-1 may contribute to the survival of proximal tubular epithelial cells under the impact of a toxic factor or oxidative stress.
{"title":"[Mechanisms of nephrotoxicity of novel anticancer compound maleimide derivative MI-1].","authors":"I. Kharchuk, O. Andrukhova, V. K. Rybal'chenko, O. Andrukhov","doi":"10.15407/FZ59.03.050","DOIUrl":"https://doi.org/10.15407/FZ59.03.050","url":null,"abstract":"The features of the impact of the maleimide derivative 1-(4-Cl-benzyl)-3-chloro-4-(CF3-fenilamino)-1H-pyrrole-2,5-dione (MI-1) on the viability and apoptosis-induced cell death of renal proximal and distal tubular epithelial cells and the amount of total and phosphorylated ERK1/2 were studied in order to establish possible mechanisms of nephrotoxicity induced by of MI-1. The viability and apoptosis of renal epithelial tubular cells after incubation with MI-1 were perfomed by 3,4,5-dymetyltiazol-2-yl-2,5-diphenyl-tetrazolium bromide (MTT)-test and by flow cytometry after staining with specific antibodies to annexin V, respectively. The amount of ERK 1/2 was determined by Western blotting. The data indicate that MI-1 was more toxic with respect to the epithelial cells of distal than proximal tubule cells. The apoptosis-induced cell death pathway is involved in the mechanisms of MI-1 cytotoxicity. One of the possible mechanisms of MI-1 nephrotoxicity is increase in phosphorylation of ERK1/2 in the distal tubules. At the same time the increase amount of total ERK1/2 in proximal tubules under the influence of MI-1 may contribute to the survival of proximal tubular epithelial cells under the impact of a toxic factor or oxidative stress.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"29 1","pages":"50-7"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85265304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effect of a 120-day -5 degrees head-down tilt (HDT) bed rest with countermeasures (physical training) on the mechanical properties of the human triceps surae muscle was studied in four healthy young women aged 28.0. The results showed that the contractile properties of the skeletal muscle studied changed considerably. After HDT without countermeasures the maximal voluntary contraction (MVC) had decreased by 36% (P < 0.05), and the electrically evoked tetanic tension at 150 Hz (P) and isometric twitch contraction (P(t)) had decreased by 24% (P < 0.05) and 12 % (P < 0.05), respectively. Time-to-peak tension (TPT) of the twitch had significantly increased by 14% (P < 0.05), but half-relaxation time (1/2 RT), and total contraction time (TCT) had decreased by 19% (P < 0.05) and 18% (P < 0.05), respectively. The difference between P(o) and MVC expressed as a percentage of P(o) and referred to as force deficiency (P(d)), was also calculated. The P(d) had increased by 40% (P < 0.001). The rate of increase of voluntary contractions calculated according to a relative scale had significantly reduced, but for the electrically evoked contraction no substantial changes were observed. After HDT with countermeasures TPT, 1/2 RT and TCT had decreased by 4%, 7%, 19%, respectively in relation to the control condition. Training had caused a decrease of 3% (P > 0.05) in MVC, and P(t) and in P(o) of 14%, and of 9% (P > 0.05), respectively. The Pd had decreased significantly by 10% (P < 0.05). The rate of increase of electrically evoked tetanic tension did not change significantly during HDT with countermeasures but the rate of increase in isometric voluntary tension development was increased. Physical training provided a reserve of neuromuscular function, which attenuated the effect of bed rest. The experimental findings indicated that neural as well as muscle adaptation occurred in response to HDT with countermeasures.
{"title":"[Influence of physical training under conditions of 120-day simulated microgravity on contractile properties and musculo-tendinous stiffness of the triceps surae muscle].","authors":"Iu A Koriak","doi":"10.15407/FZ59.02.071","DOIUrl":"https://doi.org/10.15407/FZ59.02.071","url":null,"abstract":"The effect of a 120-day -5 degrees head-down tilt (HDT) bed rest with countermeasures (physical training) on the mechanical properties of the human triceps surae muscle was studied in four healthy young women aged 28.0. The results showed that the contractile properties of the skeletal muscle studied changed considerably. After HDT without countermeasures the maximal voluntary contraction (MVC) had decreased by 36% (P < 0.05), and the electrically evoked tetanic tension at 150 Hz (P) and isometric twitch contraction (P(t)) had decreased by 24% (P < 0.05) and 12 % (P < 0.05), respectively. Time-to-peak tension (TPT) of the twitch had significantly increased by 14% (P < 0.05), but half-relaxation time (1/2 RT), and total contraction time (TCT) had decreased by 19% (P < 0.05) and 18% (P < 0.05), respectively. The difference between P(o) and MVC expressed as a percentage of P(o) and referred to as force deficiency (P(d)), was also calculated. The P(d) had increased by 40% (P < 0.001). The rate of increase of voluntary contractions calculated according to a relative scale had significantly reduced, but for the electrically evoked contraction no substantial changes were observed. After HDT with countermeasures TPT, 1/2 RT and TCT had decreased by 4%, 7%, 19%, respectively in relation to the control condition. Training had caused a decrease of 3% (P > 0.05) in MVC, and P(t) and in P(o) of 14%, and of 9% (P > 0.05), respectively. The Pd had decreased significantly by 10% (P < 0.05). The rate of increase of electrically evoked tetanic tension did not change significantly during HDT with countermeasures but the rate of increase in isometric voluntary tension development was increased. Physical training provided a reserve of neuromuscular function, which attenuated the effect of bed rest. The experimental findings indicated that neural as well as muscle adaptation occurred in response to HDT with countermeasures.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"126 1","pages":"71-84"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83244597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-01-01DOI: 10.1615/INTJPHYSPATHOPHYS.V5.I4.60
N. A. Strutyns'ka, R. B. Strutyns'kyĭ, S. Chorna, O. M. Semenykhina, L. Mys, O. Moibenko, V. F. Sahach
In experiments in vitro on the mitochondria isolated from the rat's heart we studied the effects of the openers of ATP-sensitive potassium channels (K(ATP)-channels), flocalin and tioflocalin, on the calcium-induced mitochondrial pore (MPTP) opening. Flocalin and tioflocalin caused moderate Ca(2+)-independent mitochondria swelling, which was prevented by a specific inhibitor of 5-hydroxydecanoate. This allowed to identify these compounds as mitochondrial K(ATP)-channels openers. We found that concentration-dependent inhibitory effects (10(-7) to 10(-4) M) of flocalin (with IC50 = 50 microM) and tioflocalin (with IC50 = 2,7 microM) on Ca(2+)-induced mitochondrial swelling (MPTP opening) in the heart characterized more powerful cardioprotective action of the latter. It was shown that the administration of these compounds in experiments in vivo decreased the sensitivity of the MPTP opening to Ca2+. Thus, under physiological conditions the activators K(ATP)-channels probably provide the membrane-stabilizing effects, thereby effectively increasing the organelles resistance to Ca2+, an inductor of MPTP. The results obtained allowed to characterize the role of the compound studied as cardioprotectors and regulators of the MPTP formation in the heart, indicated their anti-ischemic and anti-apoptotic effects that can be used in order to correct the mitochondrial dysfunction under pathological conditions of the cardiovascular system.
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