M. Y. Kyznietsova, T. Halenova, O. Savchuk, V. Vereschaka, L. Ostapchenko
The influence of the aqueous pods extract of kidney bean (Phaseolus vulgaris) on indicators of carbohydrate metabolism under the condition of experimental type 1 diabetes in rats was studied. It was shown that long-term oral administration of the extract at a dose of 200 mg/kg to rats leads to the decreasing of blood glucose and glycosylated hemoglobin in the background of chronic hypoinsulinemia conditions. The use of studied extract led to an increase of glycogen synthase activity in rat muscle cells and hexokinase activity in rat liver cells under the conditions of type 1 diabetes. It was estimated that administration of the aqueous extract to control rats and animals with studied model of diabetes increases GLUT-4 protein content in muscle tissue. Thus, the mechanisms of P. vulgaris hypoglycemic action can be related with the ability of the particular phytoconstituents directly effect on key intracellular elements of insulin target tissues carbohydrate metabolism under the conditions of type 1 diabetes.
{"title":"[CARBOHYDRATE METABOLISM IN TYPE 1 DIABETIC RATS UNDER THE CONDITIONS OF THE KIDNEY BEAN PODS AQUEOUS EXTRACT APPLICATION].","authors":"M. Y. Kyznietsova, T. Halenova, O. Savchuk, V. Vereschaka, L. Ostapchenko","doi":"10.15407/FZ61.06.096","DOIUrl":"https://doi.org/10.15407/FZ61.06.096","url":null,"abstract":"The influence of the aqueous pods extract of kidney bean (Phaseolus vulgaris) on indicators of carbohydrate metabolism under the condition of experimental type 1 diabetes in rats was studied. It was shown that long-term oral administration of the extract at a dose of 200 mg/kg to rats leads to the decreasing of blood glucose and glycosylated hemoglobin in the background of chronic hypoinsulinemia conditions. The use of studied extract led to an increase of glycogen synthase activity in rat muscle cells and hexokinase activity in rat liver cells under the conditions of type 1 diabetes. It was estimated that administration of the aqueous extract to control rats and animals with studied model of diabetes increases GLUT-4 protein content in muscle tissue. Thus, the mechanisms of P. vulgaris hypoglycemic action can be related with the ability of the particular phytoconstituents directly effect on key intracellular elements of insulin target tissues carbohydrate metabolism under the conditions of type 1 diabetes.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"108 7","pages":"96-103"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72560369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.1615/intjphyspathophys.v7.i4.60
E. Isaeva, O. O. Lunko, A. Romano, D. Isaev
Using an experimental model of neonatal recurrent seizures we investigated the influence of epileptic seizures in the various forms of synaptic plasticity in neurons of the somatosensory cortex. We found that early seizures do not affect the post-tetanic potentiation of the amplitude of the postsynaptic potentials and the depression of postsynaptic potentials during high-frequency stimulation. However they result in the chronic increase of the long-term potentiation of synaptic transmission. These changes of synaptic plasticity may affect the processing of the sensory information in patients with a history of recurrent seizures during early development.
{"title":"[EFFECT OF NEONATAL SEIZURES ON THE SYNAPTIC PLASTICITY OF RAT SOMATOSENSORY CORTEX].","authors":"E. Isaeva, O. O. Lunko, A. Romano, D. Isaev","doi":"10.1615/intjphyspathophys.v7.i4.60","DOIUrl":"https://doi.org/10.1615/intjphyspathophys.v7.i4.60","url":null,"abstract":"Using an experimental model of neonatal recurrent seizures we investigated the influence of epileptic seizures in the various forms of synaptic plasticity in neurons of the somatosensory cortex. We found that early seizures do not affect the post-tetanic potentiation of the amplitude of the postsynaptic potentials and the depression of postsynaptic potentials during high-frequency stimulation. However they result in the chronic increase of the long-term potentiation of synaptic transmission. These changes of synaptic plasticity may affect the processing of the sensory information in patients with a history of recurrent seizures during early development.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"190 1","pages":"11-6"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77750661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.1615/INTJPHYSPATHOPHYS.V7.I1.50
A. Kotsuruba, B. S. Kopjak, V. Sagach, N. Spivak
In experiments in vivo the effect of nanocerium (cerium oxide nanoparticles) on the stability of red blood cells to acid hemolysis, levels of both ROS and RNS generation and H2S pools in plasma and erythrocytes of old rats were investigated. In red blood cells of old rats the proton penetration into the matrix of erythrocytes showed a significant raising and the fate of labile "aging" erythrocytes in old animals compared with adult were up- regulated. These phenomena paralleled with significant up-regulation of ROS and RNS generation. Introduction for 14 days per os to old rats 0.1 mg/kg of nanocerium fully restored resistance of erythrocytes to acid hemolysis by ROS and RNS in both plasma and erythrocytes reduction. Nanocerium decreased the erythrocytes and, conversely, significantly increased the plasma's pools of H2S.
{"title":"[Nanocerium restores the erythrocytes stability to acid hemolysis by inhibition of oxygen and nitrogen reactive species in old rats].","authors":"A. Kotsuruba, B. S. Kopjak, V. Sagach, N. Spivak","doi":"10.1615/INTJPHYSPATHOPHYS.V7.I1.50","DOIUrl":"https://doi.org/10.1615/INTJPHYSPATHOPHYS.V7.I1.50","url":null,"abstract":"In experiments in vivo the effect of nanocerium (cerium oxide nanoparticles) on the stability of red blood cells to acid hemolysis, levels of both ROS and RNS generation and H2S pools in plasma and erythrocytes of old rats were investigated. In red blood cells of old rats the proton penetration into the matrix of erythrocytes showed a significant raising and the fate of labile \"aging\" erythrocytes in old animals compared with adult were up- regulated. These phenomena paralleled with significant up-regulation of ROS and RNS generation. Introduction for 14 days per os to old rats 0.1 mg/kg of nanocerium fully restored resistance of erythrocytes to acid hemolysis by ROS and RNS in both plasma and erythrocytes reduction. Nanocerium decreased the erythrocytes and, conversely, significantly increased the plasma's pools of H2S.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"1 1","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76037124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Y. Budko, N. Strutynska, I. Y. Okhay, O. M. Semenykhina, V. Sagach
It is known that mitochondria can accumulate calcium, which regulates energy metabolism and cell death. About 90% of energy of cardiomyocytes is synthesized in mitochondria. Heart cells are also affected by the rapid changes in the Ca²⁺ concentration in the cytoplasm. Therefore, mitochondrial Ca²⁺-accumulation ability is crucial. The aim of our work was to study the accumulation of Ca²⁺ in isolated rat heart mitochondria in the presence of mitochondrial potential and different extramitochondrial Ca²⁺ concentrations. Isolated organelles were loaded with fluorescent dye Fluo-4 AM (2.5 μmol/l) at a temperature of 26°C for 30 min. It has been revealed that under these conditions high mitochondrial potential was maintained sufficiently, which is necessary for the functioning of the calcium transporting system in organelles. We established that mitochondria have a limited ability to store ionized calcium, as addition of Ca²⁺ ion in concentrations of 10, 20, 50 µmol/l ensures a certain level of accumulation in organelles with further fluorescent signal growth cessation. Addition of 100 µmol/Ca²⁺ to isolated mitochondria resulted in a significant increase in fluorescence intensity (46% in the fifth minute, compared to the fluorescence when 20 µmol/l Ca²⁺ was added) and likely to activation of cation release. It was shown that ruthenium red (10⁻⁵ mol/l), an inhibitor of Ca²⁺-uniporter, prevented accumulation of calcium ions in organelles by 89%, in the presence of 100 µmol/ Ca²⁺. It was clearly seen that heart mitochondria require Mg²⁺-ATP complex (3 mmol/l) to accumulate Ca²⁺, likely to maintain the inner membrane potential, activity of Ca²⁺ uniporter and energetic processes in organelles. Thus, the process of Ca²⁺ accumulation in rat heart mitochondria requires the maintenance of mitochondrial potential, activity of Ca²⁺-uniporter, depends on extramitochondrial Ca²⁺ concentration and presence of Mg²⁺-ATP complex.
{"title":"[CA²⁺ ACCUMULATION IN ISOLATED RAT HEART MITOCHONDRIA UNDER MAINTENANCE OF MITOCHONDRIAL POTENTIAL].","authors":"A. Y. Budko, N. Strutynska, I. Y. Okhay, O. M. Semenykhina, V. Sagach","doi":"10.15407/FZ61.06.017","DOIUrl":"https://doi.org/10.15407/FZ61.06.017","url":null,"abstract":"It is known that mitochondria can accumulate calcium, which regulates energy metabolism and cell death. About 90% of energy of cardiomyocytes is synthesized in mitochondria. Heart cells are also affected by the rapid changes in the Ca²⁺ concentration in the cytoplasm. Therefore, mitochondrial Ca²⁺-accumulation ability is crucial. The aim of our work was to study the accumulation of Ca²⁺ in isolated rat heart mitochondria in the presence of mitochondrial potential and different extramitochondrial Ca²⁺ concentrations. Isolated organelles were loaded with fluorescent dye Fluo-4 AM (2.5 μmol/l) at a temperature of 26°C for 30 min. It has been revealed that under these conditions high mitochondrial potential was maintained sufficiently, which is necessary for the functioning of the calcium transporting system in organelles. We established that mitochondria have a limited ability to store ionized calcium, as addition of Ca²⁺ ion in concentrations of 10, 20, 50 µmol/l ensures a certain level of accumulation in organelles with further fluorescent signal growth cessation. Addition of 100 µmol/Ca²⁺ to isolated mitochondria resulted in a significant increase in fluorescence intensity (46% in the fifth minute, compared to the fluorescence when 20 µmol/l Ca²⁺ was added) and likely to activation of cation release. It was shown that ruthenium red (10⁻⁵ mol/l), an inhibitor of Ca²⁺-uniporter, prevented accumulation of calcium ions in organelles by 89%, in the presence of 100 µmol/ Ca²⁺. It was clearly seen that heart mitochondria require Mg²⁺-ATP complex (3 mmol/l) to accumulate Ca²⁺, likely to maintain the inner membrane potential, activity of Ca²⁺ uniporter and energetic processes in organelles. Thus, the process of Ca²⁺ accumulation in rat heart mitochondria requires the maintenance of mitochondrial potential, activity of Ca²⁺-uniporter, depends on extramitochondrial Ca²⁺ concentration and presence of Mg²⁺-ATP complex.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"25 1","pages":"17-25"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83317286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The levels of osteoassociated hormones, macroelements, interleukines, cyclic nucleotides and activity of alkaline phosphatase were being detected in the blood plasma of 74 postmenopausal women with and without osteoporosis (OP). Bone mineral density (BMD) and the metacarpal index were being assessed according to the results of densitometry. The allele polymorphisms vitamin D receptor gene (VDR) Cdx2 (rs11568820) and TaqI (rs731236) were being identified with the help of the polymerase chain reaction. The major genotype CC TaqI was found in the group without OP 3.4 times more often than with OP, while the minor genotype TT was found 3.4 times more often with the presence of OP. The presence of the genotype CC TaqI decreased the risk of OP progress by 5.5 times, and the genotype TT TaqI increased by 5.4 times. The presence of the minor allele T was associated with the higher levels of interleukin 1β, BMD and lower testosterone blood level. The major homozygote AA Cdx2 was found in women without OP 2.9 times more often, while the minor genotype GG - 5.4 times more often with the presence of OP. The presence of the major homozygote decreased the risk of OP progress by more than 5.3 times, while the minor homozygote increased the risk by 8 times. The presence of the minor allele G in the genotype was associated with the increase of parathyroid hormone, phosphorus, magnesium, alkaline phosphatase activity in blood, BMD and with the decrease of testosterone, progesterone and calcium blood levels.
{"title":"Influence of single nucleotide polymorphisms of vitamin D receptor-gene on the level of osteoassociated hormones linkage with postmenopausal osteoporosis.","authors":"Ziablitsev Ds, Larin Os","doi":"10.15407/FZ61.05.021","DOIUrl":"https://doi.org/10.15407/FZ61.05.021","url":null,"abstract":"The levels of osteoassociated hormones, macroelements, interleukines, cyclic nucleotides and activity of alkaline phosphatase were being detected in the blood plasma of 74 postmenopausal women with and without osteoporosis (OP). Bone mineral density (BMD) and the metacarpal index were being assessed according to the results of densitometry. The allele polymorphisms vitamin D receptor gene (VDR) Cdx2 (rs11568820) and TaqI (rs731236) were being identified with the help of the polymerase chain reaction. The major genotype CC TaqI was found in the group without OP 3.4 times more often than with OP, while the minor genotype TT was found 3.4 times more often with the presence of OP. The presence of the genotype CC TaqI decreased the risk of OP progress by 5.5 times, and the genotype TT TaqI increased by 5.4 times. The presence of the minor allele T was associated with the higher levels of interleukin 1β, BMD and lower testosterone blood level. The major homozygote AA Cdx2 was found in women without OP 2.9 times more often, while the minor genotype GG - 5.4 times more often with the presence of OP. The presence of the major homozygote decreased the risk of OP progress by more than 5.3 times, while the minor homozygote increased the risk by 8 times. The presence of the minor allele G in the genotype was associated with the increase of parathyroid hormone, phosphorus, magnesium, alkaline phosphatase activity in blood, BMD and with the decrease of testosterone, progesterone and calcium blood levels.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"23 1","pages":"21-7"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80833625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Goltsev, N. Babenko, A. Gaevskaya, O. Chelombitko, N. Bondarovich, T. Dubrava, M. Ostankov, V. Klochkov, N. Kavok, Y. Malyukin
Tumor development is the consequence of expanding the population of low differentiated cells with unlimited self-maintenance potential, i.e. cancer stem cells (CSCs). Application of new forms of nanocomposites capable of binding to CSCs and inducing the tumor destruction is perspective direction for treating this pathology. There have been developed the methods of obtaining hybrid nanocomplexes containing rare-earth orthovanadates GdYVO4:Eu³⁺, cholesterol and luminescent dye Dil. By immune fluorescence method using monoclonal antibodies to CD44, CD24, CD117 and Sca-1 markers there has been established the change in the ratio of tumor progenitors of various differentiation levels in a general pool of Ehrlich carcinoma (EC) after treatment with hybrid nanocomplexes. Essential reduction in the concentration of the most tumorogenic CD44high cells with simultaneous rise in the number of CD117⁺-cells resulted in an increased index of CD44high/CD117⁺ ratio. It has been demonstrated that application of hybrid nanocomplexes suppressed the tumor growth almost by 80%. The value of cooperative interactions of the cells with different phenotype signs in tumor sites has been proved. The index of CD44high/CD117⁺ ratio can be used as one of diagnostic and prognostic parameters of development and inactivation rate of tumor process when using different types of anti-tumor therapy.
{"title":"[FUNCTIONAL ACTIVITY OF EHRLICH CARCINOMA CELLS AFTER TREATMENT WITH HYBRID NANOCOMPLEXES CONTAINING ORTHOVANADATES OF RARE-EARTH ELEMENTS, CHOLESTEROL AND LUMINESCENT DYE].","authors":"A. Goltsev, N. Babenko, A. Gaevskaya, O. Chelombitko, N. Bondarovich, T. Dubrava, M. Ostankov, V. Klochkov, N. Kavok, Y. Malyukin","doi":"10.15407/FZ61.06.060","DOIUrl":"https://doi.org/10.15407/FZ61.06.060","url":null,"abstract":"Tumor development is the consequence of expanding the population of low differentiated cells with unlimited self-maintenance potential, i.e. cancer stem cells (CSCs). Application of new forms of nanocomposites capable of binding to CSCs and inducing the tumor destruction is perspective direction for treating this pathology. There have been developed the methods of obtaining hybrid nanocomplexes containing rare-earth orthovanadates GdYVO4:Eu³⁺, cholesterol and luminescent dye Dil. By immune fluorescence method using monoclonal antibodies to CD44, CD24, CD117 and Sca-1 markers there has been established the change in the ratio of tumor progenitors of various differentiation levels in a general pool of Ehrlich carcinoma (EC) after treatment with hybrid nanocomplexes. Essential reduction in the concentration of the most tumorogenic CD44high cells with simultaneous rise in the number of CD117⁺-cells resulted in an increased index of CD44high/CD117⁺ ratio. It has been demonstrated that application of hybrid nanocomplexes suppressed the tumor growth almost by 80%. The value of cooperative interactions of the cells with different phenotype signs in tumor sites has been proved. The index of CD44high/CD117⁺ ratio can be used as one of diagnostic and prognostic parameters of development and inactivation rate of tumor process when using different types of anti-tumor therapy.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"7 1","pages":"60-8"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87285309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Savchuk, M. Orlovsky, Ie. S. Iarmoliuk, S. Goncharov, V. Dosenko, G. Skibo
Functional as well as structural reorganization of brain tissues takes place in the surrounding and remotes brain areas after focal ischemic lesions. In particular, reactive or regenerative processes have been described to occur in the infarction areas and the contralateral hemisphere. Experiments were performed on 63 rats, divided into 3 groups (each consisted of 21 animals): sham operated, short-term occlusion of the right middle cerebral artery (MCAO) group, and long-term MCAO group. We have studied changes in proteasome proteolysis during transient occlusion of the middle cerebral artery using method of Koizumi J., duration 2 and 60 min and made the comparison between changes in different types of proteasome activity and severity of ischemic injury and showed three types of decrease inproteolytic activity (trypsin-, chymotrypsin-like, peptidylglutamyl peptide-hydrolyzing) in the brain tissues. Chymotrypsin-like activity of ischemic areas of the brain for short-term MCAO decreased 4.1 times compared with controls (P > 0.05), for long-term MCAO decreased 5.8 times compared with controls (P < 0.05). Trypsin-like activity of ischemic areas of brain for short-term MCAO decreased 7.1 times compared with controls (P > 0.05), for long-term MCAO decreased 12.5 times compared with controls (P < 0.05). PGPH activity of ischemic areas for short-term MCAO decreased 8 times compared with controls (P > 0.05), for long-term MCAO decreased 2.8 times compared with controls (P < 0.05). The similar dynamics was observed also in the penumbra and the core zone of the brain at 6 h of reperfusion, in the long run there is no significant difference between the core and contralateral zones. Our results suggest that proteasome activity may play also a role in contralateral cortical plasticity occurring after focal cerebral ischemia.
{"title":"Proteasomal activity in brain tissue following ischemic stroke in Wistar rats.","authors":"O. Savchuk, M. Orlovsky, Ie. S. Iarmoliuk, S. Goncharov, V. Dosenko, G. Skibo","doi":"10.15407/FZ61.05.011","DOIUrl":"https://doi.org/10.15407/FZ61.05.011","url":null,"abstract":"Functional as well as structural reorganization of brain tissues takes place in the surrounding and remotes brain areas after focal ischemic lesions. In particular, reactive or regenerative processes have been described to occur in the infarction areas and the contralateral hemisphere. Experiments were performed on 63 rats, divided into 3 groups (each consisted of 21 animals): sham operated, short-term occlusion of the right middle cerebral artery (MCAO) group, and long-term MCAO group. We have studied changes in proteasome proteolysis during transient occlusion of the middle cerebral artery using method of Koizumi J., duration 2 and 60 min and made the comparison between changes in different types of proteasome activity and severity of ischemic injury and showed three types of decrease inproteolytic activity (trypsin-, chymotrypsin-like, peptidylglutamyl peptide-hydrolyzing) in the brain tissues. Chymotrypsin-like activity of ischemic areas of the brain for short-term MCAO decreased 4.1 times compared with controls (P > 0.05), for long-term MCAO decreased 5.8 times compared with controls (P < 0.05). Trypsin-like activity of ischemic areas of brain for short-term MCAO decreased 7.1 times compared with controls (P > 0.05), for long-term MCAO decreased 12.5 times compared with controls (P < 0.05). PGPH activity of ischemic areas for short-term MCAO decreased 8 times compared with controls (P > 0.05), for long-term MCAO decreased 2.8 times compared with controls (P < 0.05). The similar dynamics was observed also in the penumbra and the core zone of the brain at 6 h of reperfusion, in the long run there is no significant difference between the core and contralateral zones. Our results suggest that proteasome activity may play also a role in contralateral cortical plasticity occurring after focal cerebral ischemia.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"15 1","pages":"11-20"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87011267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.1615/INTJPHYSPATHOPHYS.V8.I1.30
T. Vovkun, P. Yanchuk, L. Shtanova, A. Shalamay
We have investigated the action of quercetin (in a modified form--Corvitin, BCPP, Ukraine) on the rate of blood flow in the pancreas, liver and gastric mucosa of rats with acute pancreatitis (AP) caused by administration of L-arginine. The rate of blood flow was measured by hydrogen clearance method with electrochemical his generation using Polarographs Lr-9 (Czech Republic). During the first 10 days after modelling of AP in these organs it was observed a gradual decrease compared to the intact animals in the rate of blood flow by 42% (P < 0.01) in the pancreas; by 61% (P < 0.001) in the liver and by 64% (P < 0.001) in the gastric mucosa, i.e., the most significant changes occurred in the gastric mucosa, the least--in the tissue of the pancreas. Compared with the control group of animals with modelling acute pancreatitis which during 20 days was administered only saline, application of Corvitin (5 mg/kg, 1 time per day from 11 to 20 days of experiment) in varying degrees promoted to the recovery of the rate of blood flow in all investigated organs: in the pancreas--fully, in the liver--almost entirely and in the gastric mucosa--only partially. Thus, based on obtained results Corvitin can be recommended for partial or complete correction of blood flow disturbances, which arise in the pancreas and other organs of the digestive system in AP. Corvitin can improve the functional state of these organs in the early stages of the disease and accelerate the full restoration of their functions.
{"title":"[TISSUE BLOOD FLOW IN THE DIGESTIVE ORGANS OF RATS WITH ACUTE PANCREATITIS AFTER CORVITIN ADMINISTRATION].","authors":"T. Vovkun, P. Yanchuk, L. Shtanova, A. Shalamay","doi":"10.1615/INTJPHYSPATHOPHYS.V8.I1.30","DOIUrl":"https://doi.org/10.1615/INTJPHYSPATHOPHYS.V8.I1.30","url":null,"abstract":"We have investigated the action of quercetin (in a modified form--Corvitin, BCPP, Ukraine) on the rate of blood flow in the pancreas, liver and gastric mucosa of rats with acute pancreatitis (AP) caused by administration of L-arginine. The rate of blood flow was measured by hydrogen clearance method with electrochemical his generation using Polarographs Lr-9 (Czech Republic). During the first 10 days after modelling of AP in these organs it was observed a gradual decrease compared to the intact animals in the rate of blood flow by 42% (P < 0.01) in the pancreas; by 61% (P < 0.001) in the liver and by 64% (P < 0.001) in the gastric mucosa, i.e., the most significant changes occurred in the gastric mucosa, the least--in the tissue of the pancreas. Compared with the control group of animals with modelling acute pancreatitis which during 20 days was administered only saline, application of Corvitin (5 mg/kg, 1 time per day from 11 to 20 days of experiment) in varying degrees promoted to the recovery of the rate of blood flow in all investigated organs: in the pancreas--fully, in the liver--almost entirely and in the gastric mucosa--only partially. Thus, based on obtained results Corvitin can be recommended for partial or complete correction of blood flow disturbances, which arise in the pancreas and other organs of the digestive system in AP. Corvitin can improve the functional state of these organs in the early stages of the disease and accelerate the full restoration of their functions.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"73 1","pages":"53-9"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86352639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.1615/INTJPHYSPATHOPHYS.V8.I1.10
T. Dovbynchuk, L. Zakordonets, A. Putnikov, I. Vareniuk, O. Tiapko, N. Roslova, T. Sergiychuk, O. Lynchak, M. Dzerzhynsky, T. Beregova, G. Tolstanova
The aim of the present study was to investigate the effect of cephalosporin antibiotic ceftriaxone (50 mg/kg, i/m) and mac- rolide antibiotic azithromycin (15 mg/kg, per.os.) on net water transport across rat colonic epithelium. Study was done on male Wistar rats (180-250 g). Azithromycin or ceftriaxone was injected daily for 5 days. Net water transport was evaluated on the 6th day by isolated colonic loop perfusion technique in vivo on anaesthetized rats. Treatment with azithromycin increased 2,4-fold the absorption of water, while ceftriaxone caused decrease 1,9-fold water absorption. The antibiotics treatment within five days didn't change the composition of the fecal and colonic parietal microbiota. Azithromycin-induced increase in water absorption was associated with upregulation of AQP 8 water channel expression (P < 0.05) in colonic mucosa. Ceftriaxone treatment didn't change protein level of AQP8 but induced pro-inflammatory changes in colonic mucosa structure and mast cells degranulation. We showed for the first time the opposite effects ofmacrolide and cephalosporin antibiotics on net water transport across rat colonic epithelium.
{"title":"[NET WATER TRANSPORT VIA RAT COLON EPITELIUM UNDER THE EXPERIMENTAL DYSBIOSIS].","authors":"T. Dovbynchuk, L. Zakordonets, A. Putnikov, I. Vareniuk, O. Tiapko, N. Roslova, T. Sergiychuk, O. Lynchak, M. Dzerzhynsky, T. Beregova, G. Tolstanova","doi":"10.1615/INTJPHYSPATHOPHYS.V8.I1.10","DOIUrl":"https://doi.org/10.1615/INTJPHYSPATHOPHYS.V8.I1.10","url":null,"abstract":"The aim of the present study was to investigate the effect of cephalosporin antibiotic ceftriaxone (50 mg/kg, i/m) and mac- rolide antibiotic azithromycin (15 mg/kg, per.os.) on net water transport across rat colonic epithelium. Study was done on male Wistar rats (180-250 g). Azithromycin or ceftriaxone was injected daily for 5 days. Net water transport was evaluated on the 6th day by isolated colonic loop perfusion technique in vivo on anaesthetized rats. Treatment with azithromycin increased 2,4-fold the absorption of water, while ceftriaxone caused decrease 1,9-fold water absorption. The antibiotics treatment within five days didn't change the composition of the fecal and colonic parietal microbiota. Azithromycin-induced increase in water absorption was associated with upregulation of AQP 8 water channel expression (P < 0.05) in colonic mucosa. Ceftriaxone treatment didn't change protein level of AQP8 but induced pro-inflammatory changes in colonic mucosa structure and mast cells degranulation. We showed for the first time the opposite effects ofmacrolide and cephalosporin antibiotics on net water transport across rat colonic epithelium.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"19 1","pages":"76-85"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89466342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Serine protease thrombin, a key factor of blood coagulation, participates in many neuronal processes important for normal brain functioning and during pathological conditions involving abnormal neuronal synchronization, neurodegeneration and inflammation. Our previous study on CA3 pyramidal neurons showed that application ofthrombin through the activation of specific protease-activated receptor 1 (PAR1) produces a significant hyperpolarizing shift of the activation of the TTX-sensitive persistent voltage-gated Na+ current (I(Nap)) thereby affecting membrane potential and seizure threshold at the network level. It was shown that PAR1 is also expressed in CA1 area of hippocampus and can be implicated in neuronal damage in this area after status epilepticus. The aim of the present study was to evaluate the effect of thrombin on I(NaP) in CA1 pyramidal neurons from adult and young rats. Using whole cell patch-clamp technique we demonstrate that thrombin application results in the hyperpolarization shift of I(NaP) activation as well as increase in the I(NaP) amplitude in both age groups. We have found that I(NaP) in pyramidal neurons of hippocampal CA 1 region is more vulnerable to the thrombin action than I(NaP) in pyramidal neurons of hippocampal CA3 region. We have also found that the immature hippocampus is more sensitive to thrombin action which emphasizes the contribution of thrombin-dependent pathway to the regulation of neuronal activity in immature brain.
{"title":"Thrombin modulates persistent sodium current in CA1 pyramidal neurons of young and adult rat hippocampus.","authors":"O. O. Lunko, D. Isaev, O. Krishtal, E. Isaeva","doi":"10.15407/FZ61.04.005","DOIUrl":"https://doi.org/10.15407/FZ61.04.005","url":null,"abstract":"Serine protease thrombin, a key factor of blood coagulation, participates in many neuronal processes important for normal brain functioning and during pathological conditions involving abnormal neuronal synchronization, neurodegeneration and inflammation. Our previous study on CA3 pyramidal neurons showed that application ofthrombin through the activation of specific protease-activated receptor 1 (PAR1) produces a significant hyperpolarizing shift of the activation of the TTX-sensitive persistent voltage-gated Na+ current (I(Nap)) thereby affecting membrane potential and seizure threshold at the network level. It was shown that PAR1 is also expressed in CA1 area of hippocampus and can be implicated in neuronal damage in this area after status epilepticus. The aim of the present study was to evaluate the effect of thrombin on I(NaP) in CA1 pyramidal neurons from adult and young rats. Using whole cell patch-clamp technique we demonstrate that thrombin application results in the hyperpolarization shift of I(NaP) activation as well as increase in the I(NaP) amplitude in both age groups. We have found that I(NaP) in pyramidal neurons of hippocampal CA 1 region is more vulnerable to the thrombin action than I(NaP) in pyramidal neurons of hippocampal CA3 region. We have also found that the immature hippocampus is more sensitive to thrombin action which emphasizes the contribution of thrombin-dependent pathway to the regulation of neuronal activity in immature brain.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"4 1","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88949951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：