One of the most common diseases in the whole world is obesity. Every year, 18-20 thousand new cases of obesity are recorded in Ukraine. Also, the number of obese students during their studies in institutions of higher education is rapidly increasing, for whom the first year of study is the most difficult and problematic. It is known that even under normal conditions, excessive body weight requires increased energy supply and persons with insufficient weight suffer from a lack of reserves. Therefore, the purpose of our work was to study the features of the state of neurodynamic functions and regulatory processes of the heart based on indicators of heart rate variability in female students with different body weights during the first year of study at a higher education institution. This will be important for the development of individual disease prevention measures for this category of students. The study examined full-time female students (17-18 years old) of the Faculty of Natural Geography Education and Ecology of National Pedagogical Dragomanov University, who were divided into groups according to body mass index: normal, overweight, and underweight. The study was conducted on days of high mental performance under standard conditions in six stages. An electrocardiogram was registered, rhythmograms and variation pulsometry indicators were calculated, and the functional state of the central nervous system was studied using computer methods. According to the Kruskal-Wallis and ANOVA tests, the following indicators were most sensitive to the influence of body weight: moda, moda amplitude, stress index, autonomic balance index, and heart rate. According to Spearman’s non-parametric correlation analysis, at each stage of the examination, correlations between variation characteristics of heart rate variability and indicators of the state of the central nervous system were revealed, and their nature was clarified. It was established that the nature of the correlations in the studied groups was different. Thus, it was established that the deviation of body weight from the norm affects the regulatory processes of the heart and neurodynamic functions during the first year of study. Overweight and underweight female students during the first year of study had a significantly higher intension of regulatory mechanisms compared to female students with a normal body weight, so they can be classified as a group at risk of developing psychosomatic diseases.
{"title":"STATE OF NEURODYNAMIC FUNCTIONS AND REGULATORY PROCESSES OF THE HEART ACCORDING TO INDICATORS OF HEART RATE VARIABILITY IN INDIVIDUALS WITH INSUFFICIENT AND INCREASED BODY WEIGHT","authors":"O.V. Dobrostan, O.I. Plyska","doi":"10.15407/fz70.01.021","DOIUrl":"https://doi.org/10.15407/fz70.01.021","url":null,"abstract":"One of the most common diseases in the whole world is obesity. Every year, 18-20 thousand new cases of obesity are recorded in Ukraine. Also, the number of obese students during their studies in institutions of higher education is rapidly increasing, for whom the first year of study is the most difficult and problematic. It is known that even under normal conditions, excessive body weight requires increased energy supply and persons with insufficient weight suffer from a lack of reserves. Therefore, the purpose of our work was to study the features of the state of neurodynamic functions and regulatory processes of the heart based on indicators of heart rate variability in female students with different body weights during the first year of study at a higher education institution. This will be important for the development of individual disease prevention measures for this category of students. The study examined full-time female students (17-18 years old) of the Faculty of Natural Geography Education and Ecology of National Pedagogical Dragomanov University, who were divided into groups according to body mass index: normal, overweight, and underweight. The study was conducted on days of high mental performance under standard conditions in six stages. An electrocardiogram was registered, rhythmograms and variation pulsometry indicators were calculated, and the functional state of the central nervous system was studied using computer methods. According to the Kruskal-Wallis and ANOVA tests, the following indicators were most sensitive to the influence of body weight: moda, moda amplitude, stress index, autonomic balance index, and heart rate. According to Spearman’s non-parametric correlation analysis, at each stage of the examination, correlations between variation characteristics of heart rate variability and indicators of the state of the central nervous system were revealed, and their nature was clarified. It was established that the nature of the correlations in the studied groups was different. Thus, it was established that the deviation of body weight from the norm affects the regulatory processes of the heart and neurodynamic functions during the first year of study. Overweight and underweight female students during the first year of study had a significantly higher intension of regulatory mechanisms compared to female students with a normal body weight, so they can be classified as a group at risk of developing psychosomatic diseases.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"40 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140517923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adrenoreceptors play a key role in sympathetic influences on sensory neurons in normal and neuropathic conditions. We studied noradrenaline action on tonic firing in cultured rat trigeminal ganglion neurons. In a majority of the neurons (68%), Noradrenaline application had no marked effect on the firing properties. The rest of the cells (32%) were highly sensitive to noradrenaline action; they lost the ability to tonic firing and single action potential parameters significantly changed. It was established that NA-induced action on the firing is mainly due to the activation kinetics slowing of voltage-gated calcium channels. The data suggest a possibility of differential sympatho-sensory interaction in the trigeminal pathways.
{"title":"NORADRENERGIC INFLUENCE ON TONIC FIRING IN TRIGEMINAL GANGLION NEURONS","authors":"M. Telka, V. Maslov, M. Veselovsky, S.A. Fedulova","doi":"10.15407/fz70.01.014","DOIUrl":"https://doi.org/10.15407/fz70.01.014","url":null,"abstract":"Adrenoreceptors play a key role in sympathetic influences on sensory neurons in normal and neuropathic conditions. We studied noradrenaline action on tonic firing in cultured rat trigeminal ganglion neurons. In a majority of the neurons (68%), Noradrenaline application had no marked effect on the firing properties. The rest of the cells (32%) were highly sensitive to noradrenaline action; they lost the ability to tonic firing and single action potential parameters significantly changed. It was established that NA-induced action on the firing is mainly due to the activation kinetics slowing of voltage-gated calcium channels. The data suggest a possibility of differential sympatho-sensory interaction in the trigeminal pathways.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"135 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140523180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Domestic gerontology has long and glorious traditions and achievements, starting with the laureate Nobel Prize I.I. Mechnikov, O.O. Bogomolets, I.I. Schmalhausen, O.N. Severtsov, O.V. Nagorny, M.M. Horev, D.F. Chebotaryov, V.V. Frolkis A prominent place in this series of outstanding scientists is also V.V. Bezrukov (1940–2023).
{"title":"An outstanding gerontologist. Artist. Composer. In memory of Academician of the National Academy of Sciences of Ukraine Vladyslav Viktorovych Bezrukov","authors":"V. F. Sagach, M.M. Tkachenko","doi":"10.15407/fz70.01.079","DOIUrl":"https://doi.org/10.15407/fz70.01.079","url":null,"abstract":"Domestic gerontology has long and glorious traditions and achievements, starting with the laureate Nobel Prize I.I. Mechnikov, O.O. Bogomolets, I.I. Schmalhausen, O.N. Severtsov, O.V. Nagorny, M.M. Horev, D.F. Chebotaryov, V.V. Frolkis A prominent place in this series of outstanding scientists is also V.V. Bezrukov (1940–2023).","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"11 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140520196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K.Yu. Krenyov, L. I. Stepanova, N.S. Nikitina, A.I. Sukhodolya
The choice of solution for the initial infusion therapy to restore the volume of circulating blood (CBV) has been a subject of debate for many years. The purpose of our study was to compare the effect of different routes of administration of infusion solutions on CBV. The study was conducted on 250 rats weighing 200-260 g and aged 3-4 months. Animals were born from different females. The dynamics of the neutral red concentration in the gastric perfusate were measured using a 0.9% sodium chloride solution and a 10% albumin solution, which were administered in three different ways. It was established that the intravenous administration of a 0.9% sodium chloride solution at the 60th minute of the experiment reduced the concentration of an aqueous solution of neutral red in the gastric perfusate by 35%, and a 10% albumin solution by 22% compared to the control. This indicates an increase in CBV. When a 0.9% solution of sodium chloride was administered intraosseously, the concentration of an aqueous solution of neutral red significantly decreased by 33% at the 45th minute and by 44% at the 60th minute. On the other hand, under the influence of 10% albumin solution, CBV decreased by 22% at the 45th minute and by 41% at the 60th minute, but not significantly compared to the control. Therefore, the use of these solutions causes an increase in CBV in experimental animals both when administered intravenously and intraosseously, that is, it has similar hemodynamic effects. The retroperitoneal route of administration did not affect CBV changes.
{"title":"INFLUENCE OF INFUSION SOLUTIONS ON CIRCULATING BLOOD VOLUME WITH DIFFERENT ROUTES OF ADMINISTRATION INTO ANIMALS","authors":"K.Yu. Krenyov, L. I. Stepanova, N.S. Nikitina, A.I. Sukhodolya","doi":"10.15407/fz70.01.060","DOIUrl":"https://doi.org/10.15407/fz70.01.060","url":null,"abstract":"The choice of solution for the initial infusion therapy to restore the volume of circulating blood (CBV) has been a subject of debate for many years. The purpose of our study was to compare the effect of different routes of administration of infusion solutions on CBV. The study was conducted on 250 rats weighing 200-260 g and aged 3-4 months. Animals were born from different females. The dynamics of the neutral red concentration in the gastric perfusate were measured using a 0.9% sodium chloride solution and a 10% albumin solution, which were administered in three different ways. It was established that the intravenous administration of a 0.9% sodium chloride solution at the 60th minute of the experiment reduced the concentration of an aqueous solution of neutral red in the gastric perfusate by 35%, and a 10% albumin solution by 22% compared to the control. This indicates an increase in CBV. When a 0.9% solution of sodium chloride was administered intraosseously, the concentration of an aqueous solution of neutral red significantly decreased by 33% at the 45th minute and by 44% at the 60th minute. On the other hand, under the influence of 10% albumin solution, CBV decreased by 22% at the 45th minute and by 41% at the 60th minute, but not significantly compared to the control. Therefore, the use of these solutions causes an increase in CBV in experimental animals both when administered intravenously and intraosseously, that is, it has similar hemodynamic effects. The retroperitoneal route of administration did not affect CBV changes.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"41 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140519291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It's been three years since my dad has been with us - professor-pathophysiologist Vadym Yakimovich Berezovsky (1932–2020) – scientist and writer, teacher, public figure and fighter
{"title":"Thanks dad for everything... In memory of Professor Vadym Yakimovich Berezovsky","authors":"O.V. Bogomolets","doi":"10.15407/fz70.01.086","DOIUrl":"https://doi.org/10.15407/fz70.01.086","url":null,"abstract":"It's been three years since my dad has been with us - professor-pathophysiologist Vadym Yakimovich Berezovsky (1932–2020) – scientist and writer, teacher, public figure and fighter","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"58 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140519545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Long non-coding RNAs (lncRNAs) are the most numerous group of transcripts performing various functions, including the development of cardiovascular pathologies. We investigated the changes in expression of four long non-coding RNAs (H19, TUG1, GAS5, MIAT) under conditions of anoxia-reoxygenation in neonatal rat cardiomyocyte culture and ischemia-reperfusion in adult Wistar rats. A significant decrease in the expression of all four long non-coding RNAs in cell culture under experimental conditions was established. The regime of prolonged anoxia-reoxygenation led to a sharp increase in the expression level of long non-coding RNA MIAT by twice, but compared to normoxia, these changes were not significant. After ischemia-reperfusion in rat myocardium, the content of long non-coding RNA TUG1 increased by 22 times, while the expression of H19 decreased by 3.79 times, and in rat plasma, the expression of long non-coding RNA MIAT increased by 3.79 times. The obtained results allow considering long non-coding RNAs H19 and TUG1 as potential targets in ischemic myocardial injury, and MIAT as a biomarker of cardiovascular pathologies.
{"title":"CHANGES IN THE EXPRESSION OF LONG NON-CODING RNAS H19, TUG1, GAS5, MIAT DURING MYOCARDIAL ISCHEMIA-REPERFUSION","authors":"M. Khetsuriani, T. I. Drevytska, A. M. Shysh","doi":"10.15407/fz70.01.052","DOIUrl":"https://doi.org/10.15407/fz70.01.052","url":null,"abstract":"Long non-coding RNAs (lncRNAs) are the most numerous group of transcripts performing various functions, including the development of cardiovascular pathologies. We investigated the changes in expression of four long non-coding RNAs (H19, TUG1, GAS5, MIAT) under conditions of anoxia-reoxygenation in neonatal rat cardiomyocyte culture and ischemia-reperfusion in adult Wistar rats. A significant decrease in the expression of all four long non-coding RNAs in cell culture under experimental conditions was established. The regime of prolonged anoxia-reoxygenation led to a sharp increase in the expression level of long non-coding RNA MIAT by twice, but compared to normoxia, these changes were not significant. After ischemia-reperfusion in rat myocardium, the content of long non-coding RNA TUG1 increased by 22 times, while the expression of H19 decreased by 3.79 times, and in rat plasma, the expression of long non-coding RNA MIAT increased by 3.79 times. The obtained results allow considering long non-coding RNAs H19 and TUG1 as potential targets in ischemic myocardial injury, and MIAT as a biomarker of cardiovascular pathologies.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"203 5-6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140516942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M.V. Denysova, N. Strutynska, Y. Korkach, L. Mys, O.M. Magomedov, R. Strutynskyi, V. F. Sagach
Arrhythmias and congestive heart failure, which pose the greatest risk of toxic cardiomyopathy, are the clinically limiting side effects of doxorubicin, the main highly active anticancer agent. The difficulty of early diagnosis of cardiomyopathy and timely detection of cardiac dysfunction associated with chemotherapy remains a significant medical problem. The aim of our study was to identify early signs of acute doxorubicin-induced cardiotoxicity in adult rats by assessing ECG changes and biochemical parameters. Acute cardiotoxicity was modelled by short-term intraperitoneal injection of doxorubicin at a total dose of 15 mg/kg. On the 5th day of the experiment, visual fluctuations of electrocardiogram (ECG) waveforms, duration and amplitude of the main teeth and intervals, as well as heart rate (HR) in the control and experimental groups of rats were studied to determine early ECG signs of cardiotoxicity. The most significant ECG changes were a doubling of the QT interval duration and significant ST-segment elevation in the rats of the experimental group. In experiments on isolated aortic rings, we demonstrated doxorubicin-induced disruption of both vascular relaxation and contraction mechanisms. The endothelium-dependent relaxation of vascular preparations of animals after administration of doxorubicin to acetylcholine (0.1 μmol/l) was 47% less than in the control group. The vascular ring contractions in rats under the influence of norepinephrine (10 μmol/l) were 59% lower than in control rats. After doxorubicin administration, oxidative stress developed against the background of cardiovascular disorders. Thus, the content of diene conjugates and malondialdehyde increased by 4 and 2.5 times, respectively. At the same time, in isolated cardiac mitochondria, the activity of inducible NO synthase increased 3.7-fold with a simultaneous significant 4.8-fold inhibition of constitutive NO synthase. An increase in the content of acute-phase biochemical parameters that are markers of damage, namely alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatine phosphokinase myocardial fraction (CPK MB) by 2.2, 1.4 and 1.5 times, respectively, was detected. Thus, the acute cardiotoxic effect of doxorubicin results in changes in the systolic-diastolic function of the left ventricle of the heart and its conduction, automaticity and contractility, as well as impaired relaxation and contraction of isolated preparations of the aorta against the background of increased activity of inducible and decreased constitutive NO synthesis, accompanied by oxidative stress and increased content of biochemical markers of myocardial damage.
心律失常和充血性心力衰竭是中毒性心肌病的最大风险，也是多柔比星（主要的高活性抗癌药物）的临床限制性副作用。早期诊断心肌病和及时发现化疗引起的心功能不全仍然是一个重要的医学问题。我们的研究旨在通过评估心电图变化和生化指标，确定成年大鼠急性多柔比星诱发心脏毒性的早期症状。急性心脏毒性是通过短期腹腔注射总剂量为 15 毫克/千克的多柔比星来模拟的。实验第 5 天，研究了对照组和实验组大鼠心电图波形的视觉波动、主齿和间期的持续时间和振幅以及心率，以确定心脏毒性的早期心电图征兆。最明显的心电图变化是实验组大鼠的 QT 间期延长一倍，ST 段明显抬高。在离体主动脉环的实验中，我们证实了多柔比星诱导的血管松弛和收缩机制的破坏。给动物服用多柔比星后，血管制备对乙酰胆碱（0.1 μmol/l）的内皮依赖性松弛比对照组少 47%。大鼠在去甲肾上腺素（10 μmol/l）影响下的血管环收缩比对照组低 59%。服用多柔比星后，在心血管紊乱的背景下出现了氧化应激。因此，二烯共轭物和丙二醛的含量分别增加了 4 倍和 2.5 倍。与此同时，在离体的心脏线粒体中，诱导型 NO 合酶的活性增加了 3.7 倍，而组成型 NO 合酶的活性则显著抑制了 4.8 倍。作为损害标志的急性期生化指标，即丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）和肌酸磷酸激酶心肌部分（CPK MB）的含量分别增加了 2.2 倍、1.4 倍和 1.5 倍。因此，多柔比星的急性心脏毒性作用会导致心脏左心室收缩-舒张功能及其传导性、自动性和收缩性发生变化，并在诱导型 NO 合成活性增加和组成型 NO 合成活性降低的背景下，导致主动脉离体制备的松弛和收缩功能受损，同时伴有氧化应激和心肌损伤生化标志物含量增加。
{"title":"EARLY MARKERS OF ACUTE DOXORUBICIN-INDUCED CARDIOTOXICITY AND MECHANISMS OF ITS DEVELOPMENT","authors":"M.V. Denysova, N. Strutynska, Y. Korkach, L. Mys, O.M. Magomedov, R. Strutynskyi, V. F. Sagach","doi":"10.15407/fz70.01.003","DOIUrl":"https://doi.org/10.15407/fz70.01.003","url":null,"abstract":"Arrhythmias and congestive heart failure, which pose the greatest risk of toxic cardiomyopathy, are the clinically limiting side effects of doxorubicin, the main highly active anticancer agent. The difficulty of early diagnosis of cardiomyopathy and timely detection of cardiac dysfunction associated with chemotherapy remains a significant medical problem. The aim of our study was to identify early signs of acute doxorubicin-induced cardiotoxicity in adult rats by assessing ECG changes and biochemical parameters. Acute cardiotoxicity was modelled by short-term intraperitoneal injection of doxorubicin at a total dose of 15 mg/kg. On the 5th day of the experiment, visual fluctuations of electrocardiogram (ECG) waveforms, duration and amplitude of the main teeth and intervals, as well as heart rate (HR) in the control and experimental groups of rats were studied to determine early ECG signs of cardiotoxicity. The most significant ECG changes were a doubling of the QT interval duration and significant ST-segment elevation in the rats of the experimental group. In experiments on isolated aortic rings, we demonstrated doxorubicin-induced disruption of both vascular relaxation and contraction mechanisms. The endothelium-dependent relaxation of vascular preparations of animals after administration of doxorubicin to acetylcholine (0.1 μmol/l) was 47% less than in the control group. The vascular ring contractions in rats under the influence of norepinephrine (10 μmol/l) were 59% lower than in control rats. After doxorubicin administration, oxidative stress developed against the background of cardiovascular disorders. Thus, the content of diene conjugates and malondialdehyde increased by 4 and 2.5 times, respectively. At the same time, in isolated cardiac mitochondria, the activity of inducible NO synthase increased 3.7-fold with a simultaneous significant 4.8-fold inhibition of constitutive NO synthase. An increase in the content of acute-phase biochemical parameters that are markers of damage, namely alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatine phosphokinase myocardial fraction (CPK MB) by 2.2, 1.4 and 1.5 times, respectively, was detected. Thus, the acute cardiotoxic effect of doxorubicin results in changes in the systolic-diastolic function of the left ventricle of the heart and its conduction, automaticity and contractility, as well as impaired relaxation and contraction of isolated preparations of the aorta against the background of increased activity of inducible and decreased constitutive NO synthesis, accompanied by oxidative stress and increased content of biochemical markers of myocardial damage.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"24 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140520723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Investigation of the mechanism of neuronal communication underlies the fundamental discoveries that promote health. The studies of oxytocin signaling in neurons from or within different brain areas are directed to explore the role of this neurohormonal modulator in the regulation of synaptic transmission and plasticity, neuronal excitability that contributes to the reproduction, social behavior and learning capacity, anxiety, inflammation and differentiation in the brain. Oxytocin is synthesized in supraoptic and paraventricular nuclei of the hypothalamus and when secreted into the bloodstream from the posterior pituitary, it produces a significant effect on uterine contraction and lactation. At the same time this nonapeptide being released within the limbic system and brain cortex modulates neuronal activity by affecting ion channels on their membranes. The oxytocin receptor is primarily coupled to Gq/11 proteins causing phospholipase C activation, Ca2+ release and store-operated Ca2+ entry. These pathways are central for the regulation of the activity of different types of TRP channels, especially of the canonical subfamily (TRPC). Here we highlight the link between oxytocin signaling, which is particularly well investigated in the myometrium, and receptor-operated TRPC4 and multimodal TRPV4 ion channels that participate in oxytocin-dependent regulation of the uterine smooth muscle contractility under various conditions. Importantly, similarly to oxytocin, these channels have been implicated in neuropathic pain behavior, anxiety, fear and depression. Since similar signal transduction pathways are likely to be functional in neuronal cells, we propose that future studies of oxytocin effects in the CNS should also consider the role of these Ca2+-permeable channels.
{"title":"https://fz.kiev.ua/index.php?abs=2034","authors":"O. Moroz, O. Basovska, A. Zholos","doi":"10.15407/fz70.01.067","DOIUrl":"https://doi.org/10.15407/fz70.01.067","url":null,"abstract":"Investigation of the mechanism of neuronal communication underlies the fundamental discoveries that promote health. The studies of oxytocin signaling in neurons from or within different brain areas are directed to explore the role of this neurohormonal modulator in the regulation of synaptic transmission and plasticity, neuronal excitability that contributes to the reproduction, social behavior and learning capacity, anxiety, inflammation and differentiation in the brain. Oxytocin is synthesized in supraoptic and paraventricular nuclei of the hypothalamus and when secreted into the bloodstream from the posterior pituitary, it produces a significant effect on uterine contraction and lactation. At the same time this nonapeptide being released within the limbic system and brain cortex modulates neuronal activity by affecting ion channels on their membranes. The oxytocin receptor is primarily coupled to Gq/11 proteins causing phospholipase C activation, Ca2+ release and store-operated Ca2+ entry. These pathways are central for the regulation of the activity of different types of TRP channels, especially of the canonical subfamily (TRPC). Here we highlight the link between oxytocin signaling, which is particularly well investigated in the myometrium, and receptor-operated TRPC4 and multimodal TRPV4 ion channels that participate in oxytocin-dependent regulation of the uterine smooth muscle contractility under various conditions. Importantly, similarly to oxytocin, these channels have been implicated in neuropathic pain behavior, anxiety, fear and depression. Since similar signal transduction pathways are likely to be functional in neuronal cells, we propose that future studies of oxytocin effects in the CNS should also consider the role of these Ca2+-permeable channels.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140515832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Asanov, V. Shatilo, I. Dyba, I. Antonyuk-Shcheglova, S. Naskalova, O. Bondarenko
Military stress has a traumatic effect on mental and physical health, and disrupts the adaptive capabilities of the body, in particular, its ability to adequately respond to hypoxia. However, the specifics of the reaction of the cardiorespiratory system to dosed hypoxia in individuals who experiencing warrelated chronic stress (WRCS) have not yet been elucidated. The peculiarities of the reaction of the cardiorespiratory system to dosed hypoxia in women experiencing WRCS were clarified. It was shown that during breathing of atmospheric air, the blood saturation level, lung ventilation indicators, and hemodynamics did not differ significantly between women exposed to WRCS and those not exposed to it. However, during a controlled hypoxic test (breathing a gas mixture with 12% oxygen for 20 min), women experiencing WRCS experienced a more significant decrease in blood saturation, indicating a lower tolerance to hypoxia. This is also evidenced by a reduced lung ventilation response to controlled hypoxia in the WRCS group, where the increase in ventilation primarily occurred due to an elevation in breathing rate rather than tidal volume. At the same time, the hemodynamic response to hypoxia in women with WRCS was characterized by a more substantial increase in heart rate and systolic arterial pressure. Thus, it was established that in women who were in the state of HCV, the body’s resistance to the effects of hypoxia decreases. At the same time, in conditions of hypoxia, the nature of their breathing changes, and the compensatory growth of pulmonary ventilation decreases, but the growth of hemodynamic indicators increases.
{"title":"RESPONSE TO CONTROLLED HYPOXIA IN WOMEN EXPERIENCING WAR-RELATED CHRONIC STRESS","authors":"E. Asanov, V. Shatilo, I. Dyba, I. Antonyuk-Shcheglova, S. Naskalova, O. Bondarenko","doi":"10.15407/fz69.06.015","DOIUrl":"https://doi.org/10.15407/fz69.06.015","url":null,"abstract":"Military stress has a traumatic effect on mental and physical health, and disrupts the adaptive capabilities of the body, in particular, its ability to adequately respond to hypoxia. However, the specifics of the reaction of the cardiorespiratory system to dosed hypoxia in individuals who experiencing warrelated chronic stress (WRCS) have not yet been elucidated. The peculiarities of the reaction of the cardiorespiratory system to dosed hypoxia in women experiencing WRCS were clarified. It was shown that during breathing of atmospheric air, the blood saturation level, lung ventilation indicators, and hemodynamics did not differ significantly between women exposed to WRCS and those not exposed to it. However, during a controlled hypoxic test (breathing a gas mixture with 12% oxygen for 20 min), women experiencing WRCS experienced a more significant decrease in blood saturation, indicating a lower tolerance to hypoxia. This is also evidenced by a reduced lung ventilation response to controlled hypoxia in the WRCS group, where the increase in ventilation primarily occurred due to an elevation in breathing rate rather than tidal volume. At the same time, the hemodynamic response to hypoxia in women with WRCS was characterized by a more substantial increase in heart rate and systolic arterial pressure. Thus, it was established that in women who were in the state of HCV, the body’s resistance to the effects of hypoxia decreases. At the same time, in conditions of hypoxia, the nature of their breathing changes, and the compensatory growth of pulmonary ventilation decreases, but the growth of hemodynamic indicators increases.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"53 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139280436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Mylostуva, B. Gutyj, V. Borshenko, O. Marenkov, O. Yaremko, O. Lesnovska, O. zhboldina, R. Mylostyvyi, S. Farafonov, T. Levytskyy, H. Kushnir, H. Ryvak
Fatty acids have a significant impact on the overall metabolic processes in the body. One of the links of the general metabolism is the formation of peroxidation products during the oxidation of fatty acids. The aim of our work was to investigate the relationship between the content of fatty acids and lipid peroxidation products under copper, selenium, and manganese deficiency. The study was conducted on calves of the Ukrainian meat breed aged 6 months. An appropriate dose of deficient trace elements was added to the basic diet of the experimental groups. Determination of the spectrum of fatty acids in blood serum was performed using a gas chromatograph. The diet with copper, selenium and manganese increased the content of short-chain saturated fatty acids and the level of monounsaturated fatty acids decreased. The obtained results indicate the influence of individual fatty acids on the formation of peroxidation of products as a result of increased oxidation of the corresponding substrates. It is shown that deficient copper, selenium and manganese affect the correlation between fatty acids and peroxidation products. The revealed regularities can become the basis for the development of sound strategies for the mineral nutrition of beef cattle by including deficient trace elements and polyunsaturated omega fatty acids. In addition to obtaining important data on the relationship between biochemical processes in the body of beef cattle, the study of the influence of dietary strategies on the nutritional value and fatty acid composition of beef is provided by our further research.
{"title":"CONTENT OF FATTY ACIDS AND LIPID PEROXIDATION PRODUCTS IN THE BLOOD SERUM OF YOUNG UKRAINIAN BEEF BREED UNDER THE ACTION SALT OF COPPER, SELENIUM AND MANGANESE","authors":"D. Mylostуva, B. Gutyj, V. Borshenko, O. Marenkov, O. Yaremko, O. Lesnovska, O. zhboldina, R. Mylostyvyi, S. Farafonov, T. Levytskyy, H. Kushnir, H. Ryvak","doi":"10.15407/fz69.06.060","DOIUrl":"https://doi.org/10.15407/fz69.06.060","url":null,"abstract":"Fatty acids have a significant impact on the overall metabolic processes in the body. One of the links of the general metabolism is the formation of peroxidation products during the oxidation of fatty acids. The aim of our work was to investigate the relationship between the content of fatty acids and lipid peroxidation products under copper, selenium, and manganese deficiency. The study was conducted on calves of the Ukrainian meat breed aged 6 months. An appropriate dose of deficient trace elements was added to the basic diet of the experimental groups. Determination of the spectrum of fatty acids in blood serum was performed using a gas chromatograph. The diet with copper, selenium and manganese increased the content of short-chain saturated fatty acids and the level of monounsaturated fatty acids decreased. The obtained results indicate the influence of individual fatty acids on the formation of peroxidation of products as a result of increased oxidation of the corresponding substrates. It is shown that deficient copper, selenium and manganese affect the correlation between fatty acids and peroxidation products. The revealed regularities can become the basis for the development of sound strategies for the mineral nutrition of beef cattle by including deficient trace elements and polyunsaturated omega fatty acids. In addition to obtaining important data on the relationship between biochemical processes in the body of beef cattle, the study of the influence of dietary strategies on the nutritional value and fatty acid composition of beef is provided by our further research.","PeriodicalId":12307,"journal":{"name":"Fiziolohichnyĭ zhurnal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139280732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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