首页 > 最新文献

FEMS microbiology ecology最新文献

英文 中文
Dissolved organic phosphorus bond-class utilization by Synechococcus. Synechococcus 对溶解有机磷键类的利用。
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-08-13 DOI: 10.1093/femsec/fiae099
Emily M Waggoner, Kahina Djaoudi, Julia M Diaz, Solange Duhamel

Dissolved organic phosphorus (DOP) contains compounds with phosphoester, phosphoanhydride, and phosphorus-carbon bonds. While DOP holds significant nutritional value for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by Synechococcus strains from open and coastal oceans. Both strains exhibited comparable growth rates when provided phosphate, a phosphoanhydride [3-polyphosphate and 45-polyphosphate], or a DOP compound with both phosphoanhydride and phosphoester bonds (adenosine 5'-triphosphate). Growth rates on phosphoesters [glucose-6-phosphate, adenosine 5'-monophosphate, bis(4-methylumbelliferyl) phosphate] were variable, and neither strain grew on selected phosphorus-carbon compounds. Both strains hydrolyzed 3-polyphosphate, then adenosine 5'-triphosphate, and lastly adenosine 5'-monophosphate, exhibiting preferential enzymatic hydrolysis of phosphoanhydride bonds. The strains' exoproteomes contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of phosphoanhydride bonds by these exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity toward the phosphoanhydride 3-polyphosphate, with varying affinities between strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition and highlight varied growth and enzymatic responses to molecular diversity within DOP bond-classes, thereby expanding our understanding of microbially mediated DOP cycling in marine ecosystems.

溶解有机磷(DOP)包含具有磷酯、磷酸酐和磷碳键的化合物。虽然 DOP 对海洋微生物具有重要的营养价值,但对于广泛分布的蓝藻球藻来说,每种键类的生物利用率在很大程度上仍是未知数。本研究评估了来自开阔海洋和近海的 Synechococcus 菌株对特定键类 DOP 的利用情况。当提供磷酸盐、磷酸酐(3-聚磷酸盐、45-聚磷酸盐)或同时具有磷酸酐和磷酯键的 DOP 化合物(5'-三磷酸腺苷)时,两种菌株的生长率相当。在磷酸酯(6-磷酸葡萄糖、5'-单磷酸腺苷、双(4-甲基伞形酮基)磷酸酯)上的生长率各不相同,两种菌株都不在选定的磷碳化合物上生长。两株菌株都先水解 3-聚磷酸盐,然后是 5'-三磷酸腺苷,最后是 5'-单磷酸腺苷,表现出优先水解磷酸酐键的酶解作用。这些菌株的外蛋白组含有磷水解酶,再加上在磷酸盐缺乏的情况下,3-聚磷酸盐和 5'-三磷酸腺苷的无细胞水解作用增强,表明这些外蛋白对磷酸酐键的矿化作用活跃。Synechococcus 碱性磷酸酶具有广泛的底物特异性,包括对磷酸酐 3-多聚磷酸的活性,不同菌株之间的亲和力不同。总之,这些发现强调了具有磷酸酐键的化合物在球藻磷营养中的潜在重要作用,并突出了对 DOP 键类分子多样性的不同生长和酶反应,从而扩大了我们对海洋生态系统中微生物介导的 DOP 循环的了解。
{"title":"Dissolved organic phosphorus bond-class utilization by Synechococcus.","authors":"Emily M Waggoner, Kahina Djaoudi, Julia M Diaz, Solange Duhamel","doi":"10.1093/femsec/fiae099","DOIUrl":"10.1093/femsec/fiae099","url":null,"abstract":"<p><p>Dissolved organic phosphorus (DOP) contains compounds with phosphoester, phosphoanhydride, and phosphorus-carbon bonds. While DOP holds significant nutritional value for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by Synechococcus strains from open and coastal oceans. Both strains exhibited comparable growth rates when provided phosphate, a phosphoanhydride [3-polyphosphate and 45-polyphosphate], or a DOP compound with both phosphoanhydride and phosphoester bonds (adenosine 5'-triphosphate). Growth rates on phosphoesters [glucose-6-phosphate, adenosine 5'-monophosphate, bis(4-methylumbelliferyl) phosphate] were variable, and neither strain grew on selected phosphorus-carbon compounds. Both strains hydrolyzed 3-polyphosphate, then adenosine 5'-triphosphate, and lastly adenosine 5'-monophosphate, exhibiting preferential enzymatic hydrolysis of phosphoanhydride bonds. The strains' exoproteomes contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of phosphoanhydride bonds by these exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity toward the phosphoanhydride 3-polyphosphate, with varying affinities between strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition and highlight varied growth and enzymatic responses to molecular diversity within DOP bond-classes, thereby expanding our understanding of microbially mediated DOP cycling in marine ecosystems.</p>","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11319936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Total RNA analysis of the active microbiome on moving bed biofilm reactor carriers under incrementally increasing micropollutant concentrations. 在微污染物浓度逐渐增加的情况下,对移动床生物膜反应器载体上的活性微生物群进行总 RNA 分析。
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-08-13 DOI: 10.1093/femsec/fiae098
Joseph Donald Martin, Selina Tisler, Maria Scheel, Sif Svendsen, Muhammad Zohaib Anwar, Athanasios Zervas, Flemming Ekelund, Kai Bester, Lars Hestbjerg Hansen, Carsten Suhr Jacobsen, Lea Ellegaard-Jensen

Micropollutants are increasingly prevalent in the aquatic environment. A major part of these originates from wastewater treatment plants since traditional treatment technologies do not remove micropollutants sufficiently. Moving bed biofilm reactors (MBBRs), however, have been shown to aid in micropollutant removal when applied to conventional wastewater treatment as a polishing step. Here, we used Total RNA sequencing to investigate both the active microbial community and functional dynamics of MBBR biofilms when these were exposed to increasing micropollutant concentrations over time. Concurrently, we conducted batch culture experiments using biofilm carriers from the MBBRs to assess micropollutant degradation potential. Our study showed that biofilm eukaryotes, in particular protozoa, were negatively influenced by micropollutant exposure, in contrast to prokaryotes that increased in relative abundance. Further, we found several functional genes that were differentially expressed between the MBBR with added micropollutants and the control. These include genes involved in aromatic and xenobiotic compound degradation. Moreover, the biofilm carrier batch experiment showed vastly different alterations in benzotriazole and diclofenac degradation following the increased micropollutant concentrations in the MBBR. Ultimately, this study provides essential insights into the microbial community and functional dynamics of MBBRs and how an increased load of micropollutants influences these dynamics.

微污染物在水生环境中越来越普遍。由于传统的处理技术无法充分去除微污染物,因此这些微污染物主要来自废水处理厂。然而,移动床生物膜反应器(MBBRs)作为一个抛光步骤应用于传统废水处理时,已被证明有助于去除微污染物。在此,我们利用总 RNA 测序技术研究了移动床生物膜反应器生物膜在微污染物浓度不断增加的情况下的活性微生物群落和功能动态。同时,我们使用 MBBR 生物膜载体进行了批量培养实验,以评估微污染物的降解潜力。我们的研究表明,生物膜真核生物,尤其是原生动物,受到微污染物暴露的负面影响,而相对丰度增加的原核生物则相反。此外,我们还发现,在添加了微污染物的 MBBR 和对照组之间,有几个功能基因的表达存在差异。其中包括参与芳香族和异生物化合物降解的基因。此外,生物膜载体批次实验表明,随着 MBBR 中微污染物浓度的增加,苯并三唑和双氯芬酸的降解发生了巨大的变化。最终,这项研究提供了有关 MBBR 微生物群落和功能动态以及微污染物负荷增加如何影响这些动态的重要见解。
{"title":"Total RNA analysis of the active microbiome on moving bed biofilm reactor carriers under incrementally increasing micropollutant concentrations.","authors":"Joseph Donald Martin, Selina Tisler, Maria Scheel, Sif Svendsen, Muhammad Zohaib Anwar, Athanasios Zervas, Flemming Ekelund, Kai Bester, Lars Hestbjerg Hansen, Carsten Suhr Jacobsen, Lea Ellegaard-Jensen","doi":"10.1093/femsec/fiae098","DOIUrl":"10.1093/femsec/fiae098","url":null,"abstract":"<p><p>Micropollutants are increasingly prevalent in the aquatic environment. A major part of these originates from wastewater treatment plants since traditional treatment technologies do not remove micropollutants sufficiently. Moving bed biofilm reactors (MBBRs), however, have been shown to aid in micropollutant removal when applied to conventional wastewater treatment as a polishing step. Here, we used Total RNA sequencing to investigate both the active microbial community and functional dynamics of MBBR biofilms when these were exposed to increasing micropollutant concentrations over time. Concurrently, we conducted batch culture experiments using biofilm carriers from the MBBRs to assess micropollutant degradation potential. Our study showed that biofilm eukaryotes, in particular protozoa, were negatively influenced by micropollutant exposure, in contrast to prokaryotes that increased in relative abundance. Further, we found several functional genes that were differentially expressed between the MBBR with added micropollutants and the control. These include genes involved in aromatic and xenobiotic compound degradation. Moreover, the biofilm carrier batch experiment showed vastly different alterations in benzotriazole and diclofenac degradation following the increased micropollutant concentrations in the MBBR. Ultimately, this study provides essential insights into the microbial community and functional dynamics of MBBRs and how an increased load of micropollutants influences these dynamics.</p>","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11385203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbial anabolic and catabolic utilization of hydrocarbons in deep subseafloor sediments of Guaymas Basin. 瓜伊马斯盆地深层海底沉积物中微生物对碳氢化合物的合成代谢和分解代谢利用。
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae093
Toshiki Nagakura, Yuki Morono, Motoo Ito, Kai Mangelsdorf, Stefanie Pötz, Ellen Schnabel, Jens Kallmeyer

Guaymas Basin, located in the Gulf of California, is a hydrothermally active marginal basin. Due to steep geothermal gradients and localized heating by sill intrusions, microbial substrates like short-chain fatty acids and hydrocarbons are abiotically produced from sedimentary organic matter at comparatively shallow depths. We analyzed the effect of hydrocarbons on uptake of hydrocarbons by microorganisms via nano-scale secondary ion mass spectrometry (NanoSIMS) and microbial sulfate reduction rates (SRR), using samples from two drill sites sampled by IODP Expedition 385 (U1545C and U1546D). These sites are in close proximity of each other (ca. 1 km) and have very similar sedimentology. Site U1546D experienced the intrusion of a sill that has since then thermally equilibrated with the surrounding sediment. Both sites currently have an identical geothermal gradient, despite their different thermal history. The localized heating by the sill led to thermal cracking of sedimentary organic matter and formation of potentially bioavailable organic substrates. There were low levels of hydrocarbon and nitrogen uptake in some samples from both sites, mostly in surficial samples. Hydrocarbon and methane additions stimulated SRR in near-seafloor samples from Site U1545C, while samples from Site U1546D reacted positively only on methane. Our data indicate the potential of microorganisms to metabolize hydrocarbons even in the deep subsurface of Guaymas Basin.

瓜伊马斯盆地位于加利福尼亚湾外海,是一个热液活跃的边缘盆地。由于陡峭的地热梯度和页岩侵入的局部加热,微生物基质(如短链脂肪酸和碳氢化合物)在相对较浅的深度从沉积有机物中产生。我们利用 IODP Exp. 385(U1545C 和 U1546D)取样的两个钻探地点的样本,通过纳米级二次离子质谱(NanoSIMS)和微生物硫酸盐还原率(SRR)分析了碳氢化合物对微生物吸收碳氢化合物的影响。这两个地点相距很近(约 1 公里),沉积学非常相似。U1546D 岩石经历了一个山体的侵入,此后与周围的沉积物发生了热平衡。尽管热历史不同,但这两个地点目前的地热梯度相同。山体的局部加热导致了沉积有机物的热裂解,并形成了潜在的生物有机基质。在这两个地点的一些样本中,碳氢化合物和氮的吸收水平较低,主要是在表层样本中。碳氢化合物和甲烷的添加刺激了 U1545C 站点近海底样本中的 SRR,而 U1546D 站点的样本只对甲烷产生了正反应。我们的数据表明,即使在瓜伊马斯盆地的深层地下,微生物也有代谢碳氢化合物的潜力。
{"title":"Microbial anabolic and catabolic utilization of hydrocarbons in deep subseafloor sediments of Guaymas Basin.","authors":"Toshiki Nagakura, Yuki Morono, Motoo Ito, Kai Mangelsdorf, Stefanie Pötz, Ellen Schnabel, Jens Kallmeyer","doi":"10.1093/femsec/fiae093","DOIUrl":"10.1093/femsec/fiae093","url":null,"abstract":"<p><p>Guaymas Basin, located in the Gulf of California, is a hydrothermally active marginal basin. Due to steep geothermal gradients and localized heating by sill intrusions, microbial substrates like short-chain fatty acids and hydrocarbons are abiotically produced from sedimentary organic matter at comparatively shallow depths. We analyzed the effect of hydrocarbons on uptake of hydrocarbons by microorganisms via nano-scale secondary ion mass spectrometry (NanoSIMS) and microbial sulfate reduction rates (SRR), using samples from two drill sites sampled by IODP Expedition 385 (U1545C and U1546D). These sites are in close proximity of each other (ca. 1 km) and have very similar sedimentology. Site U1546D experienced the intrusion of a sill that has since then thermally equilibrated with the surrounding sediment. Both sites currently have an identical geothermal gradient, despite their different thermal history. The localized heating by the sill led to thermal cracking of sedimentary organic matter and formation of potentially bioavailable organic substrates. There were low levels of hydrocarbon and nitrogen uptake in some samples from both sites, mostly in surficial samples. Hydrocarbon and methane additions stimulated SRR in near-seafloor samples from Site U1545C, while samples from Site U1546D reacted positively only on methane. Our data indicate the potential of microorganisms to metabolize hydrocarbons even in the deep subsurface of Guaymas Basin.</p>","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11250447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microeukaryote community coalescence strengthens community stability and elevates diversity. 微核生物群落的凝聚加强了群落的稳定性并提高了多样性。
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae100
Máté Vass, Anna J Székely, Ulla Carlsson-Graner, Johan Wikner, Agneta Andersson

Mixing of entire microbial communities represents a frequent, yet understudied phenomenon. Here, we mimicked estuarine condition in a microcosm experiment by mixing a freshwater river community with a brackish sea community and assessed the effects of both environmental and community coalescences induced by varying mixing processes on microeukaryotic communities. Signs of shifted community composition of coalesced communities towards the sea parent community suggest asymmetrical community coalescence outcome, which, in addition, was generally less impacted by environmental coalescence. Community stability, inferred from community cohesion, differed among river and sea parent communities, and increased following coalescence treatments. Generally, community coalescence increased alpha diversity and promoted competition from the introduction (or emergence) of additional (or rare) species. These competitive interactions in turn had community stabilizing effect as evidenced by the increased proportion of negative cohesion. The fate of microeukaryotes was influenced by mixing ratios and frequencies (i.e. one-time versus repeated coalescence). Namely, diatoms were negatively impacted by coalescence, while fungi, ciliates, and cercozoans were promoted to varying extents, depending on the mixing ratios of the parent communities. Our study suggests that the predictability of coalescence outcomes was greater when the sea parent community dominated the final community, and this predictability was further enhanced when communities collided repeatedly.

整个微生物群落的混合是一种经常发生但研究不足的现象。在这里,我们在微观世界实验中模拟了河口条件,将淡水河群落与咸海群落混合,并评估了不同混合过程引起的环境和群落凝聚对微真核生物群落的影响。聚合群落的群落组成向海洋母群落转移的迹象表明,群落聚合的结果是不对称的,此外,环境聚合对海洋母群落的影响一般较小。从群落内聚力推断出的群落稳定性在河流和海洋母群落之间存在差异,并在群落凝聚处理后有所增加。一般来说,群落凝聚会增加α多样性,并促进引入(或出现)更多(或稀有)物种所带来的竞争。这些竞争性相互作用反过来又具有稳定群落的作用,负内聚力比例的增加就是证明。微真核细胞的命运受混合比例和频率(即一次性与重复凝聚)的影响。也就是说,硅藻受到凝聚的负面影响,而真菌、纤毛虫和纤毛虫则在不同程度上得到促进,这取决于母群落的混合比例。我们的研究表明,当海洋母群落在最终群落中占主导地位时,凝聚结果的可预测性更高;当群落反复碰撞时,这种可预测性进一步增强。
{"title":"Microeukaryote community coalescence strengthens community stability and elevates diversity.","authors":"Máté Vass, Anna J Székely, Ulla Carlsson-Graner, Johan Wikner, Agneta Andersson","doi":"10.1093/femsec/fiae100","DOIUrl":"10.1093/femsec/fiae100","url":null,"abstract":"<p><p>Mixing of entire microbial communities represents a frequent, yet understudied phenomenon. Here, we mimicked estuarine condition in a microcosm experiment by mixing a freshwater river community with a brackish sea community and assessed the effects of both environmental and community coalescences induced by varying mixing processes on microeukaryotic communities. Signs of shifted community composition of coalesced communities towards the sea parent community suggest asymmetrical community coalescence outcome, which, in addition, was generally less impacted by environmental coalescence. Community stability, inferred from community cohesion, differed among river and sea parent communities, and increased following coalescence treatments. Generally, community coalescence increased alpha diversity and promoted competition from the introduction (or emergence) of additional (or rare) species. These competitive interactions in turn had community stabilizing effect as evidenced by the increased proportion of negative cohesion. The fate of microeukaryotes was influenced by mixing ratios and frequencies (i.e. one-time versus repeated coalescence). Namely, diatoms were negatively impacted by coalescence, while fungi, ciliates, and cercozoans were promoted to varying extents, depending on the mixing ratios of the parent communities. Our study suggests that the predictability of coalescence outcomes was greater when the sea parent community dominated the final community, and this predictability was further enhanced when communities collided repeatedly.</p>","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11287207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A common ericoid shrub modulates the diversity and structure of fungal communities across an arbuscular to ectomycorrhizal tree dominance gradient. 一种常见的啮齿类灌木调节了从树根到外生菌根树木优势梯度上真菌群落的多样性和结构。
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae092
Alexander Polussa, Elisabeth B Ward, Mark A Bradford, Angela M Oliverio

Differences between arbuscular (AM) and ectomycorrhizal (EcM) trees strongly influence forest ecosystem processes, in part through their impact on saprotrophic fungal communities. Ericoid mycorrhizal (ErM) shrubs likely also impact saprotrophic communities given that they can shape nutrient cycling by slowing decomposition rates and intensifying nitrogen limitation. We investigated the depth distributions of saprotrophic and EcM fungal communities in paired subplots with and without a common understory ErM shrub, mountain laurel (Kalmia latifolia L.), across an AM to EcM tree dominance gradient in a temperate forest by analyzing soils from the organic, upper mineral (0-10 cm), and lower mineral (cumulative depth of 30 cm) horizons. The presence of K. latifolia was strongly associated with the taxonomic and functional composition of saprotrophic and EcM communities. Saprotrophic richness was consistently lower in the Oa horizon when this ErM shrub species was present. However, in AM tree-dominated plots, the presence of the ErM shrub was associated with a higher relative abundance of saprotrophs. Given that EcM trees suppress both the diversity and relative abundance of saprotrophic communities, our results suggest that separate consideration of ErM shrubs and EcM trees may be necessary when assessing the impacts of plant mycorrhizal associations on belowground communities.

乔木(AM)和外生菌根(EcM)树木之间的差异会对森林生态系统过程产生强烈影响,部分原因是它们会影响营养繁殖真菌群落。Ericoid菌根(ErM)灌木可能也会影响营养盐循环,因为它们可以通过减缓分解速度和加强氮限制来影响营养盐循环。我们通过分析温带森林中有机层、上矿物层(0-10 厘米)和下矿物层(累计深度 30 厘米)的土壤,研究了有和没有常见林下 ErM 灌木山月桂(Kalmia latifolia L.)的成对小地块中,从 AM 到 EcM 树木优势梯度上的嗜渍真菌和 EcM 真菌群落的深度分布。K.latifolia的存在与根瘤菌和外生菌根群落的分类和功能组成密切相关。当存在这种 ErM 灌木物种时,Oa 地层中的嗜渍生物丰富度一直较低。然而,在以 AM 树为主的地块中,ErM 灌木的存在与较高的嗜畔生物相对丰度有关。鉴于生态管理树抑制了嗜渍生物群落的多样性和相对丰度,我们的研究结果表明,在评估植物菌根关联对地下群落的影响时,有必要分别考虑 ErM 灌木和生态管理树。
{"title":"A common ericoid shrub modulates the diversity and structure of fungal communities across an arbuscular to ectomycorrhizal tree dominance gradient.","authors":"Alexander Polussa, Elisabeth B Ward, Mark A Bradford, Angela M Oliverio","doi":"10.1093/femsec/fiae092","DOIUrl":"10.1093/femsec/fiae092","url":null,"abstract":"<p><p>Differences between arbuscular (AM) and ectomycorrhizal (EcM) trees strongly influence forest ecosystem processes, in part through their impact on saprotrophic fungal communities. Ericoid mycorrhizal (ErM) shrubs likely also impact saprotrophic communities given that they can shape nutrient cycling by slowing decomposition rates and intensifying nitrogen limitation. We investigated the depth distributions of saprotrophic and EcM fungal communities in paired subplots with and without a common understory ErM shrub, mountain laurel (Kalmia latifolia L.), across an AM to EcM tree dominance gradient in a temperate forest by analyzing soils from the organic, upper mineral (0-10 cm), and lower mineral (cumulative depth of 30 cm) horizons. The presence of K. latifolia was strongly associated with the taxonomic and functional composition of saprotrophic and EcM communities. Saprotrophic richness was consistently lower in the Oa horizon when this ErM shrub species was present. However, in AM tree-dominated plots, the presence of the ErM shrub was associated with a higher relative abundance of saprotrophs. Given that EcM trees suppress both the diversity and relative abundance of saprotrophic communities, our results suggest that separate consideration of ErM shrubs and EcM trees may be necessary when assessing the impacts of plant mycorrhizal associations on belowground communities.</p>","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11250453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141456184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Substrate complexity buffers negative interactions in a synthetic community of leaf litter degraders. 底物的复杂性可缓冲枯落叶降解生物合成群落中的负面相互作用。
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae102
Parmis Abdoli, Clément Vulin, Miriam Lepiz, Alexander B Chase, Claudia Weihe, Alejandra Rodríguez-Verdugo

Leaf litter microbes collectively degrade plant polysaccharides, influencing land-atmosphere carbon exchange. An open question is how substrate complexity-defined as the structure of the saccharide and the amount of external processing by extracellular enzymes-influences species interactions. We tested the hypothesis that monosaccharides (i.e. xylose) promote negative interactions through resource competition, and polysaccharides (i.e. xylan) promote neutral or positive interactions through resource partitioning or synergism among extracellular enzymes. We assembled a three-species community of leaf litter-degrading bacteria isolated from a grassland site in Southern California. In the polysaccharide xylan, pairs of species stably coexisted and grew equally in coculture and in monoculture. Conversely, in the monosaccharide xylose, competitive exclusion and negative interactions prevailed. These pairwise dynamics remained consistent in a three-species community: all three species coexisted in xylan, while only two species coexisted in xylose, with one species capable of using peptone. A mathematical model showed that in xylose these dynamics could be explained by resource competition. Instead, the model could not predict the coexistence patterns in xylan, suggesting other interactions exist during biopolymer degradation. Overall, our study shows that substrate complexity influences species interactions and patterns of coexistence in a synthetic microbial community of leaf litter degraders.

叶丛微生物集体降解植物多糖,影响陆地与大气的碳交换。一个悬而未决的问题是,底物的复杂性(即糖的结构和细胞外酶的外部加工量)如何影响物种间的相互作用。我们检验了这样一个假设:单糖(如木糖)通过资源竞争促进负面相互作用,而多糖(如木聚糖)通过资源分配或细胞外酶之间的协同作用促进中性或正面相互作用。我们组建了一个从南加州草地分离出来的三品种落叶降解细菌群落。在多糖木聚糖中,成对物种稳定共存,在共培养和单培养中同样生长。相反,在单糖木糖中,竞争排斥和负作用占主导地位。这些成对动态在三物种群落中保持一致:所有三个物种在木聚糖中共存,而只有两个物种在木糖中共存,其中一个物种能够使用蛋白胨。数学模型显示,在木糖中,这些动态可以用资源竞争来解释。相反,该模型无法预测木糖中的共存模式,这表明在生物聚合物降解过程中还存在其他相互作用。总之,我们的研究表明,底物的复杂性会影响枯落叶降解合成微生物群落中物种间的相互作用和共存模式。
{"title":"Substrate complexity buffers negative interactions in a synthetic community of leaf litter degraders.","authors":"Parmis Abdoli, Clément Vulin, Miriam Lepiz, Alexander B Chase, Claudia Weihe, Alejandra Rodríguez-Verdugo","doi":"10.1093/femsec/fiae102","DOIUrl":"10.1093/femsec/fiae102","url":null,"abstract":"<p><p>Leaf litter microbes collectively degrade plant polysaccharides, influencing land-atmosphere carbon exchange. An open question is how substrate complexity-defined as the structure of the saccharide and the amount of external processing by extracellular enzymes-influences species interactions. We tested the hypothesis that monosaccharides (i.e. xylose) promote negative interactions through resource competition, and polysaccharides (i.e. xylan) promote neutral or positive interactions through resource partitioning or synergism among extracellular enzymes. We assembled a three-species community of leaf litter-degrading bacteria isolated from a grassland site in Southern California. In the polysaccharide xylan, pairs of species stably coexisted and grew equally in coculture and in monoculture. Conversely, in the monosaccharide xylose, competitive exclusion and negative interactions prevailed. These pairwise dynamics remained consistent in a three-species community: all three species coexisted in xylan, while only two species coexisted in xylose, with one species capable of using peptone. A mathematical model showed that in xylose these dynamics could be explained by resource competition. Instead, the model could not predict the coexistence patterns in xylan, suggesting other interactions exist during biopolymer degradation. Overall, our study shows that substrate complexity influences species interactions and patterns of coexistence in a synthetic microbial community of leaf litter degraders.</p>","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11289631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of turbulence on diatoms of the genus Pseudo-nitzschia spp. and associated bacteria. 湍流对假尼氏硅藻属硅藻及相关细菌的影响
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae094
Yanis Maire, François G Schmitt, Konstantinos Kormas, Sotirios Vasileiadis, Amandine Caruana, Dimitra-Ioli Skouroliakou, Vasileios Bampouris, Lucie Courcot, Fabienne Hervé, Muriel Crouvoisier, Urania Christaki

Turbulence is one of the least investigated environmental factors impacting the ecophysiology of phytoplankton, both at the community and individual species level. Here, we investigated, for the first time, the effect of a turbulence gradient (Reynolds number, from Reλ = 0 to Reλ = 360) on two species of the marine diatom Pseudo-nitzschia and their associated bacterial communities under laboratory conditions. Cell abundance, domoic acid (DA) production, chain formation, and Chl a content of P. fraudulenta and P. multiseries were higher for intermediate turbulence (Reλ = 160 or 240). DA was detectable only in P. multiseries samples. These observations were supported by transcriptomic analyses results, which suggested the turbulence related induction of the expression of the DA production locus, with a linkage to an increased photosynthetic activity of the total metatranscriptome. This study also highlighted a higher richness of the bacterial community associated with the nontoxic strain of P. fraudulenta in comparison to the toxic strain of P. multiseries. Bacillus was an important genus in P. multiseries cultures (relative abundance 15.5%) and its highest abundances coincided with the highest DA levels. However, associated bacterial communities of both Pseudo-nitzschia species did not show clear patterns relative to turbulence intensity.

湍流是影响浮游植物生态生理学的环境因素中最少被研究的因素之一,无论是在群落层面还是个体物种层面。在这里,我们首次研究了湍流梯度(${rm{Reynolds/number}}$,从${rm{R}}{rm{e}}}_{rm{lambda }} = 0$到${rm{R}}{rm{e}}}_{rm{lambda }} = 360$)在实验室条件下对两种海洋硅藻假尼茨藻及其相关细菌群落的影响。在中等湍流度(${rm{R}}{{rm{e}}}_{rm{lambda }} = 160 美元或 240 美元)条件下,欺诈硅藻(P. fraudulenta)和多重硅藻(P. multiseries)的细胞丰度、多莫酸(DA)产量、链形成和 Chl a 含量都较高。只有在P. multiseries样本中才能检测到DA。这些观察结果得到了转录组分析结果的支持,转录组分析结果表明,湍流诱导了 DA 生产基因座的表达,并与总元转录组光合作用活性的增加有关。这项研究还突出表明,与多毒菌属无毒菌株相比,与诈囊藻无毒菌株相关的细菌群落更为丰富。芽孢杆菌(Bacillus)是多毒藻培养物中的重要菌属(相对丰度为 15.5%),其最高丰度与最高 DA 水平相吻合。然而,两种假尼茨藻的相关细菌群落并没有显示出与湍流强度相关的明显模式。
{"title":"Effects of turbulence on diatoms of the genus Pseudo-nitzschia spp. and associated bacteria.","authors":"Yanis Maire, François G Schmitt, Konstantinos Kormas, Sotirios Vasileiadis, Amandine Caruana, Dimitra-Ioli Skouroliakou, Vasileios Bampouris, Lucie Courcot, Fabienne Hervé, Muriel Crouvoisier, Urania Christaki","doi":"10.1093/femsec/fiae094","DOIUrl":"10.1093/femsec/fiae094","url":null,"abstract":"<p><p>Turbulence is one of the least investigated environmental factors impacting the ecophysiology of phytoplankton, both at the community and individual species level. Here, we investigated, for the first time, the effect of a turbulence gradient (Reynolds number, from Reλ = 0 to Reλ = 360) on two species of the marine diatom Pseudo-nitzschia and their associated bacterial communities under laboratory conditions. Cell abundance, domoic acid (DA) production, chain formation, and Chl a content of P. fraudulenta and P. multiseries were higher for intermediate turbulence (Reλ = 160 or 240). DA was detectable only in P. multiseries samples. These observations were supported by transcriptomic analyses results, which suggested the turbulence related induction of the expression of the DA production locus, with a linkage to an increased photosynthetic activity of the total metatranscriptome. This study also highlighted a higher richness of the bacterial community associated with the nontoxic strain of P. fraudulenta in comparison to the toxic strain of P. multiseries. Bacillus was an important genus in P. multiseries cultures (relative abundance 15.5%) and its highest abundances coincided with the highest DA levels. However, associated bacterial communities of both Pseudo-nitzschia species did not show clear patterns relative to turbulence intensity.</p>","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of successive summer drought periods on bacterial diversity along a plant species richness gradient. 夏季连续干旱期对植物物种丰富度梯度上细菌多样性的影响
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae096
Yuri Pinheiro Alves de Souza, Roberto Siani, Cynthia Albracht, Yuanyuan Huang, Nico Eisenhauer, Anja Vogel, Cameron Wagg, Michael Schloter, Stefanie Schulz

Drought is a major stressor to soil microbial communities, and the intensification of climate change is predicted to increase hydric stress worldwide in the coming decades. As a possible mitigating factor for the consequences of prolonged drought periods, above and belowground biodiversity can increase ecosystem resistance and resilience by improving metabolic redundancy and complementarity as biodiversity increases. Here, we investigated the interaction effect between plant richness and successive, simulated summer drought on soil microbial communities during a period of 9 years.To do that, we made use of a well-established biodiversity experiment (The Jena Experiment) to investigate the response of microbial richness and community composition to successive drought periods alongside a plant richness gradient, which covers 1-, 2-, 4-, 8-, 16-, and 60-species plant communities. Plots were covered from natural precipitation by installing rain shelters 6 weeks every summer. Bulk soil samples were collected 1 year after the last summer drought was simulated. Our data indicate that bacterial richness increased after successive exposure to drought, with the increase being stable along the plant richness gradient. We identified a significant effect of plant species richness on the soil microbial community composition and determined the taxa significantly impacted by drought at each plant richness level. Our data successfully demonstrates that summer drought might have a legacy effect on soil bacterial communities.

干旱是土壤微生物群落的主要压力源,而气候变化的加剧预计将在未来几十年内增加全球的水文压力。作为缓解长期干旱后果的一个可能因素,地上和地下生物多样性可以随着生物多样性的增加而提高代谢冗余和互补性,从而增强生态系统的抵抗力和恢复力。为此,我们利用一个成熟的生物多样性实验(耶拿实验)来研究微生物丰富度和群落组成对连续干旱期和植物丰富度梯度的响应,植物丰富度梯度包括 1 种、2 种、4 种、8 种、16 种和 60 种植物群落。每年夏季,通过安装遮雨棚对地块进行为期 6 周的覆盖,以防止自然降水。在模拟最后一次夏季干旱 1 年后收集大量土壤样本。我们的数据表明,在连续遭受干旱后,细菌的丰富度有所增加,而且沿植物丰富度梯度的增加是稳定的。我们确定了植物物种丰富度对土壤微生物群落组成的显著影响,并确定了在每个植物丰富度水平上受干旱显著影响的类群。我们的数据成功地证明了夏季干旱可能会对土壤细菌群落产生遗留影响。
{"title":"The effect of successive summer drought periods on bacterial diversity along a plant species richness gradient.","authors":"Yuri Pinheiro Alves de Souza, Roberto Siani, Cynthia Albracht, Yuanyuan Huang, Nico Eisenhauer, Anja Vogel, Cameron Wagg, Michael Schloter, Stefanie Schulz","doi":"10.1093/femsec/fiae096","DOIUrl":"10.1093/femsec/fiae096","url":null,"abstract":"<p><p>Drought is a major stressor to soil microbial communities, and the intensification of climate change is predicted to increase hydric stress worldwide in the coming decades. As a possible mitigating factor for the consequences of prolonged drought periods, above and belowground biodiversity can increase ecosystem resistance and resilience by improving metabolic redundancy and complementarity as biodiversity increases. Here, we investigated the interaction effect between plant richness and successive, simulated summer drought on soil microbial communities during a period of 9 years.To do that, we made use of a well-established biodiversity experiment (The Jena Experiment) to investigate the response of microbial richness and community composition to successive drought periods alongside a plant richness gradient, which covers 1-, 2-, 4-, 8-, 16-, and 60-species plant communities. Plots were covered from natural precipitation by installing rain shelters 6 weeks every summer. Bulk soil samples were collected 1 year after the last summer drought was simulated. Our data indicate that bacterial richness increased after successive exposure to drought, with the increase being stable along the plant richness gradient. We identified a significant effect of plant species richness on the soil microbial community composition and determined the taxa significantly impacted by drought at each plant richness level. Our data successfully demonstrates that summer drought might have a legacy effect on soil bacterial communities.</p>","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial: thematic issue on microbial ecotoxicology. 编辑:微生物生态毒理学专题。
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae097
Stéphane Vuilleumier, Lise Barthelmebs, Natàlia Corcoll, Marina Hery, Dimitrios G Karpouzas, Lukas Y Wick
{"title":"Editorial: thematic issue on microbial ecotoxicology.","authors":"Stéphane Vuilleumier, Lise Barthelmebs, Natàlia Corcoll, Marina Hery, Dimitrios G Karpouzas, Lukas Y Wick","doi":"10.1093/femsec/fiae097","DOIUrl":"10.1093/femsec/fiae097","url":null,"abstract":"","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11271680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatio-temporal changes of small protist and free-living bacterial communities in a temperate dimictic lake: insights from metabarcoding and machine learning. 温带二叠纪湖泊中小型原生生物和自由生活细菌群落的时空变化:代谢标码和机器学习的启示。
IF 3.5 3区 生物学 Q2 MICROBIOLOGY Pub Date : 2024-07-12 DOI: 10.1093/femsec/fiae104
Michał Karlicki, Anna Bednarska, Paweł Hałakuc, Kacper Maciszewski, Anna Karnkowska

Microbial communities, which include prokaryotes and protists, play an important role in aquatic ecosystems and influence ecological processes. To understand these communities, metabarcoding provides a powerful tool to assess their taxonomic composition and track spatio-temporal dynamics in both marine and freshwater environments. While marine ecosystems have been extensively studied, there is a notable research gap in understanding eukaryotic microbial communities in temperate lakes. Our study addresses this gap by investigating the free-living bacteria and small protist communities in Lake Roś (Poland), a dimictic temperate lake. Metabarcoding analysis revealed that both the bacterial and protist communities exhibit distinct seasonal patterns that are not necessarily shaped by dominant taxa. Furthermore, machine learning and statistical methods identified crucial amplicon sequence variants (ASVs) specific to each season. In addition, we identified a distinct community in the anoxic hypolimnion. We have also shown that the key factors shaping the composition of analysed community are temperature, oxygen, and silicon concentration. Understanding these community structures and the underlying factors is important in the context of climate change potentially impacting mixing patterns and leading to prolonged stratification.

包括原核生物和原生生物在内的微生物群落在水生生态系统中发挥着重要作用,并影响着生态过程。为了了解这些群落,代谢标码提供了一种强大的工具来评估它们的分类组成,并跟踪海洋和淡水环境中的时空动态。虽然对海洋生态系统进行了广泛的研究,但在了解温带湖泊中的真核微生物群落方面还存在明显的研究空白。我们的研究通过调查罗兹湖(波兰)这一二纬温带湖泊中的自由生活细菌和小型原生生物群落,填补了这一空白。元条码分析表明,细菌和原生生物群落都表现出独特的季节性模式,这些模式并不一定是由优势类群形成的。此外,机器学习和统计方法还发现了每个季节特有的关键扩增子序列变异(ASV)。此外,我们还在缺氧的下盐层中发现了一个独特的群落。我们还发现,影响所分析群落组成的关键因素是温度、氧气和硅浓度。在气候变化可能影响混合模式并导致长期分层的背景下,了解这些群落结构及其背后的因素非常重要。
{"title":"Spatio-temporal changes of small protist and free-living bacterial communities in a temperate dimictic lake: insights from metabarcoding and machine learning.","authors":"Michał Karlicki, Anna Bednarska, Paweł Hałakuc, Kacper Maciszewski, Anna Karnkowska","doi":"10.1093/femsec/fiae104","DOIUrl":"10.1093/femsec/fiae104","url":null,"abstract":"<p><p>Microbial communities, which include prokaryotes and protists, play an important role in aquatic ecosystems and influence ecological processes. To understand these communities, metabarcoding provides a powerful tool to assess their taxonomic composition and track spatio-temporal dynamics in both marine and freshwater environments. While marine ecosystems have been extensively studied, there is a notable research gap in understanding eukaryotic microbial communities in temperate lakes. Our study addresses this gap by investigating the free-living bacteria and small protist communities in Lake Roś (Poland), a dimictic temperate lake. Metabarcoding analysis revealed that both the bacterial and protist communities exhibit distinct seasonal patterns that are not necessarily shaped by dominant taxa. Furthermore, machine learning and statistical methods identified crucial amplicon sequence variants (ASVs) specific to each season. In addition, we identified a distinct community in the anoxic hypolimnion. We have also shown that the key factors shaping the composition of analysed community are temperature, oxygen, and silicon concentration. Understanding these community structures and the underlying factors is important in the context of climate change potentially impacting mixing patterns and leading to prolonged stratification.</p>","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11302952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
FEMS microbiology ecology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1