The increasing demand for natural and nutritional edible coating material for preserving fruits and vegetables has become a big challenge for the research community. The present study is the first to develop an edible coating using guava pomace protein and oil-based oleogel by incorporating electro-spun bacterial cellulose nanofiber as a stabilizer in the preservation of tomatoes. The shear thinning behavior, pseudoplastic properties, and average particle size of 779.2 ± 1.16–2967.1 ± 0.17 nm images of the developed edible coating demonstrated its physical stability for coating application. Simultaneous thermal analysis results were in the range of denaturation temperature from 91.20 to 109.57 °C, and the enthalpy (ΔH) of 282.532–302.186 J/g revealed thermal stability. SDS-PAGE analysis of the Invitro gastrointestinal tract digestibility study shows protein digestibility up to 88.03 ± 0.71 % with a reduction of molecular size from ∼75 kDa to 45 kDa. Among the six different combinations, edible coating developed with 50:50 % (w/w) of oleogel and micronized nanofiber revealed the highest preservation quality of tomatoes for 21 days with higher firmness of 25.88 ± 0.12 %, low redness value (a*/b*) of 0.95 ± 0.01, pH of 4.4 ± 0.02, decreased total soluble solids of 5.31 ± 0.01 %, and reduced weight loss of 9.86 ± 0.46 %. Thus, the developed guava pomace protein-oil-based edible coating exhibits potential properties for fruit and vegetable preservation.
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