The present study explored the characterization, antibacterial and snakehead fish preservation effects of active films containing compounded cinnamon and thyme essential oils (v:v, 8:2; CTEO). CTEO exhibited synergistic inhibition against Staphylococcus aureus, while additive effects against Pseudomonas aeruginosa, Shewanella putrefaciens, Aeromonas sobria, and Escherichia coli. The 14 % CTEO active film displayed reduced water vapor permeability and water solubility, increased total color difference, denser network structure, enhanced tensile strength and thermal stability. FTIR analysis suggested the successful integration of CTEO into the starch matrix. Moreover, the CTEO film exhibited promising scavenging activity against DPPH·and antibacterial properties against five prevalent putrefactive bacteria. During cold storage, packaging with CTEO film improved the water-holding capacity, elasticity, and resilience of snakehead fish but triggered the diminution of pH, TVB-N, TBARS, TNC, and putrefactive bacteria. Significant correlations were observed between Proteobacteria abundance and pH, TVB-N, and TBARS values of snakehead fish. Thus, CTEO shows potential as a material for active films.
An accelerated in situ (AIS) light exposure and measurement technology for prediction of retail light protection performance of soymilk packaging is reported. The ability of a packaging material to protect light sensitive marker solutions, either comprising riboflavin (RF) or chlorophyll-a (CHLA), from an intense light source was determined. The light-induced decay of the marker was tracked with in situ spectroscopy and modeled to quantify the light protection performance of each packaging material. For a set of packages, chemical analyses (RF, thiobarbituric acid reactive substances (TBARS), hexanal) of packaged soymilk under Simulated Retail (SR) storage conditions demonstrated excellent correlation to the light protection performance data of the same package set determined by AIS (R2 > 0.97). The use of AIS enables rapid quantification of soymilk packaging material light protection performance and the associated preservation of soymilk nutrient and sensory properties, thus it may be applied to improve the design of soymilk packaging materials for light protection performance and contribute towards the improvement of packaged soymilk product shelf life.
To extend the shelf life of konjac gels, this study investigated the effects of preservatives prepared using food production byproducts. First, the ultrasonic and microwave-assisted extraction of huajiao seed protein (HSP) was performed using the following process parameters: ultrasonic power of 90 W, ultrasonic time of 1 min, microwave power of 100 W, and microwave time of 1.5 min. The HSP extraction rate under this parameter reached 56.36 ± 1.91 %. The huajiao seed antimicrobial peptide (HSAP) obtained via enzymatic hydrolysis of the HSP inhibited Escherichia coli and Staphylococcus aureus by 20.26 ± 3.79 % and 28.97 ± 4.00 %, respectively. HSAP was mixed with Lactobacillus plantarum-fermented soy whey (LPFSW) and inulin to develop an LPFSW composite preservative for konjac gel preservation. The selected LPFSW composite preservative exhibited excellent moisturizing properties and was stable at 4 °C for 10 d with inhibition rates of 96.85 ± 0.45 % and 94.94 ± 1.57 % against E. coli and S. aureus, respectively. In addition, at 4 °C, the LPFSW composite preservative was able to control the weight loss rate of the konjac gel to < 10 % in 8 days and maintain its textural properties, appearance, and odor. In summary, the LPFSW composite preservative was effective in preserving the konjac gel, extending its shelf life from 2 to 8 d.
Antimicrobial packaging film mediated by photodynamic inactivation (PDI) based on biodegradable polymers is promising. The photosensitizer berberine chloride (BBR) was encapsulated into the β-cyclodextrin (CD) to exert its aggregation-induced emission (AIE) property. On this basis, novel PDI-mediated polyvinyl alcohol (PVA) films were fabricated by incorporating the CD/BBR inclusion complex. The PVA/CD/BBR films had high light transmittance (∼70 %) in the visible light range, and possessed good biodegradability with the water absorption of 76 % and water solubility of 31 %. Besides, the films exhibited good mechanical properties with the tensile strength of 62.53 MPa at elongation break of 69.14 %, owing to the formation of hydrogen bonds between the PVA and CD/BBR. The PDI-mediated films were irradiated (14.4 J/cm2) to produce ROS to kill ∼4 log CFU/mL of Listeria monocytogenes and Vibrio parahaemolyticus. In addition, the films potently inactivated native bacteria (0.92 log CFU/g) on salmon fillets after 9 days of storage, and maintained their sensory quality, water holding capacity, and notably, inhibited the chemical changes of salmon fillets to extend the shelf-life for at least 3 days.
The increasing emphasis on food quality and consumer safety has prompted researchers to explore green manufacturing techniques for packaging materials. In this study, γ-cyclodextrin metal-organic frameworks (γ-CD-MOFs) were prepared as promising nanocarriers for gallic acid (GA) and silver nanoparticles (Ag NPs). These nanocarriers (GA-Ag@CD-MOFs) were further incorporated into a zein matrix to fabricate multifunctional composite films, referred to as MOFs-Zein. Scanning electron microscopy (SEM) and atomic force microscope (AFM) analysis indicated that the fabricated composite film exhibited excellent biocompatibility, with GA-Ag@CD-MOFs uniformly dispersed within the film matrix. Fourier transform infrared spectroscopy (FT-IR) result revealed that the incorporation of GA-Ag@CD-MOFs facilitated the formation of intermolecular hydrogen bonds with the matrix polymer. The addition of GA-Ag@CD-MOFs enhanced the tensile strength of the composite film by 19.70 %, improved the water barrier performance by 57.76 %, and provided almost 100 % UV protection. The exceptional controlled release capability of γ-CD-MOFs for GA empowered the composite films to maintain their robust initial antioxidant capacity even after 100 days of storage. Moreover, the composite films performed high-efficiency antibacterial activity against Escherichia coli O157:H7 and Salmonella enterica. Acute toxicological studies showed no histopathology related toxicity was found in GA-Ag@CD-MOFs treated mice. When applied to fresh pork, the composite films significantly reduced the key quality indicators of raw meat, compared to the control film. Overall, this work presents a promising approach to fabricate the composite films as a sustainable and functional solution for green functional food packaging.
A new active packaging composed of poly(e-caprolactone) (PCL) and thermoplastic gliadin proteins incorporating green tea extract (GTE) was developed through compounding and film-extrusion process. Firstly, commercial green tea extract was analyzed, revealing that GTE primarily comprised epigallocatechin gallate (EGCG), which conferred potent antioxidant activity determined by the DPPH assay, along with moderate antibacterial activity. The incorporation of 5 wt% of GTE in the extruded films increased thermal stability and Young’s Modulus, and reduced oxygen and water vapor permeability with respect to control PCL/TPG film. Migration studies showed that the release of GTE depended on the type of food simulant, with higher levels observed in an oil-in-water emulsion simulant compared to a non-acidic aqueous food simulant, while no migration of GTE components was detected in dry foodstuffs simulant. Consequently, the antioxidant capacity derived from migrated GTE components were considerably higher in 50 % ethanol than in 10 % ethanol. Furthermore, PCL/TPG-GTE film exhibited antibacterial activity against Gram negative E. coli and Gram positive S. aureus in vitro when tested following the JIS Z2801 standard. The GTE addition also improved walnuts stability, resulting in a reduction and delay of fat peroxidation. Hence, the novel active system developed in this work can constitute a sustainable alternative to improve quality and safety of packaged products.
Carbon dioxide (CO2) laser technology was used to modify the gas permeability of polybutylene succinate (PBS) film by creating holes. The holes were made using pulse fluences ranging from 37.0 to 369.8 J cm−2 and pulse lengths varying from 20 to 200 µs. The minimum fluence required to cause deformation in the PBS film was 129.4 J cm−2, resulting in a tiny opening at the perforation site. Aperture size and pulse duration have an impact on gas exchange because they increase the surface area of microholes and facilitate gas transfer. The efficacy of banana packing was evaluated by employing microperforated PBS films with different number of holes and a pulse length of 200 µs. The lowest CO2 and highest oxygen (O2) concentrations were observed in PBS 7/200, followed by 5/200, 3/200, and 1/200, respectively. The gas composition with the highest potential was PBS 7/200, indicating its effectiveness in preserving banana quality. Perforating PBS film packaging with a CO2 laser allows for regulated gas permeability, which has the potential to improve the quality and safety of food.
There is growing emphasis on developing biopolymer-based food packaging materials to improve food quality, reduce food waste, and decrease the risk of foodborne diseases. In this study, cinnamaldehyde (CIN), a natural antimicrobial essential oil, was encapsulated within porous vaterite CaCO3 (CA) nanoparticles. The encapsulation efficiency and loading capacity of the cinnamaldehyde in the optimized CA-CIN nanoparticles was 88.9 ± 1.3 % and 9.7 ± 1.4 %, respectively. The release of the essential oil from the CA-CIN nanoparticles was triggered under acidic aqueous conditions, indicating they exhibited water/pH release properties. The minimum inhibitory concentration (MIC) of the CA-CIN nanoparticles against both E. coli and S. aureus was 2 mg/mL. In vitro cell culture studies suggested that the CA-CIN nanoparticles exhibited some cytotoxicity (IC50 = 87 ± 11 μg/mL), indicating that further in vivo toxicity studies are required. A sodium alginate-carboxymethyl cellulose (SC) composite film containing 6 % CA-CIN showed good UV-blocking, water-resistance, and mechanical properties. These films also exhibited good antibacterial activity, extending the shelf life of pork by 2–4 days at 4 ℃. These novel biopolymer-based films may be useful for food preservation applications.