Backgrounds: Carrot pomace is a by-product of the juice industry, which quickly spoils due to its high moisture content and is usually considered waste. However, it is a valuable product containing a significant amount of bioactive compounds, particularly carotenoids.Context and purpose of this study:In this study, we aimed to develop a technology for obtaining carrot powder from carrot pomace with an increased availability of beta-carotene. The unique aspect of the technology involved the use of enzymatic and impact-disintegration-activation processing (IDAP) of the raw material to enhance the release of beta-carotene from plant cells.Objective of this study:To achieve the goal, we have set objectives to compare various dosages of enzyme preparation and to evaluate devices for grinding carrots that can be used to make powder. At all stages, it was necessary to measure the amount of beta-carotene in the samples.Results: It has been found that the yield of carotenoids from carrot pomace is 185% higher compared to whole carrots. Moreover, the application of enzymatic and impact-disintegration-activation processing significantly increases the carotenoid yield in the powder by 17 times compared to carrots (200% in terms of dry matter). This remarkable result was achieved through the described processing method and the selection of the enzyme preparation. Additionally, the mechanoactivation obtained by using the Desi-15 disintegrator played a crucial role. It was also observed that the use of impact-disintegration-activation processing for further grinding of dried carrots can increase the beta-carotene yield by 8 times. Conclusions: The carrot pomace was processed, resulting in a fine-dispersed carrot powder that shows promising applications in the food or agricultural industries.Keywords: Carrot pomace, beta-carotene, impact-disintegrator-activator processing (IDAP), enzymatic processing, pectolytic enzyme preparations
{"title":"Unlocking the potential of carrot pomace: Enzymatic and impact-disintegrator-activator processing for elevated beta-carotene concentration in carrot powder","authors":"Angelina Baskovtceva, Nadezhda Barakova, Evgeniy Samodelkin, Elena Kiprushkina, Reem Alkhateeb, Grigory Tochilnikov","doi":"10.31989/ffhd.v13i10.1184","DOIUrl":"https://doi.org/10.31989/ffhd.v13i10.1184","url":null,"abstract":"Backgrounds: Carrot pomace is a by-product of the juice industry, which quickly spoils due to its high moisture content and is usually considered waste. However, it is a valuable product containing a significant amount of bioactive compounds, particularly carotenoids.Context and purpose of this study:In this study, we aimed to develop a technology for obtaining carrot powder from carrot pomace with an increased availability of beta-carotene. The unique aspect of the technology involved the use of enzymatic and impact-disintegration-activation processing (IDAP) of the raw material to enhance the release of beta-carotene from plant cells.Objective of this study:To achieve the goal, we have set objectives to compare various dosages of enzyme preparation and to evaluate devices for grinding carrots that can be used to make powder. At all stages, it was necessary to measure the amount of beta-carotene in the samples.Results: It has been found that the yield of carotenoids from carrot pomace is 185% higher compared to whole carrots. Moreover, the application of enzymatic and impact-disintegration-activation processing significantly increases the carotenoid yield in the powder by 17 times compared to carrots (200% in terms of dry matter). This remarkable result was achieved through the described processing method and the selection of the enzyme preparation. Additionally, the mechanoactivation obtained by using the Desi-15 disintegrator played a crucial role. It was also observed that the use of impact-disintegration-activation processing for further grinding of dried carrots can increase the beta-carotene yield by 8 times. Conclusions: The carrot pomace was processed, resulting in a fine-dispersed carrot powder that shows promising applications in the food or agricultural industries.Keywords: Carrot pomace, beta-carotene, impact-disintegrator-activator processing (IDAP), enzymatic processing, pectolytic enzyme preparations","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135366282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-20DOI: 10.31989/ffhd.v13i10.1213
Marvin Heuer, Christopher Baker, Michael Sedlak, Kim Jin Woo, Kim Hyun Kee, Debasis Bagchi
Background: Oxidative damage has emerged as one of the significant factors in the onset of neurodegenerative disorders, primarily owing to a) higher usage of oxygen by brain cells and b) higher amounts of lipids in the neuronal membranes. Together, these have resulted in higher susceptibility of brain cells to ROS-induced lipid peroxidation, damage to other cellular macromolecules, including nucleic acids and proteins, and death of neurons by apoptosis, ferroptosis, and necrosis. The design of therapeutics to prevent oxidative damage-induced brain stroke or other irreversible injuries has remained a challenge owing to the continuous production of free radicals at the site of damage. In this respect, plant-derived tannins and tannic acid derivatives have exhibited promising results in scavenging free radicals and inhibiting lipid peroxidation.Objective: This investigation reports the effect of Ghala Rois extract (GRE), a methyl gallate enriched gall formed on the nutgall sumac tree, Rhus javanica L. (Anacardiaceae), on enhancing the cognitive function and overall well-being through a double-blind, placebo-controlled, randomized, parallel-group design study on 80 healthy adult volunteers over a period of six consecutive weeks.Results: Administration of GRE in the dosage of 750 mg/day p.o. (with an equal amount of corn starch as a placebo) after breakfast resulted in significant improvement in cognitive functions as assessed by HappyNeuroPro, a digital therapy tool, and the cognitive tools under Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MOCA) and Adult ADHD Self-Reported Scale (ASRSv1.1). Concomitantly, the GRE-treated subjects also scored significantly higher (p = 0.033) in the roles of Physical and Emotional Problems, Energy/Fatigue, Emotional well-being, and pain as evaluated by linearly independent pairwise comparisons among the estimated marginal means and computed using alpha = 0.05. In the Global Evaluation Questionnaire, over 77% of the treated subjects believed that the product increased their attention span, and helped their memory by 70%, and they also indicated their willingness to buy the commercially available GRE. Evaluation of the complete metabolic profile in the GRE-treated subjects significantly lowered the blood urea nitrogen (BUN) (11.98 mg/dL) as compared to placebo (13.27 mg/dL) (p = 0.011) as well as SGPT/ALT from 28.09 U/L (placebo) to 18.17 U/L (GRE-treated) (p<0.001). However, the vital parameters, including heart rate and systolic/diastolic pressure, were not affected by the consumption of GRE extract.Conclusion: The study demonstrated that GRE could be used as an effective new phytotherapeutic for reversing oxidative damage-induced neuronal degeneration and improving cognitive health in these study individuals.Keywords: Galla Rhois extract (GRE); Rhus javanica L. (family Anacardiaceae); Cognitive functions; ADHD; Executive function; Verbal memory; Safety, Reactive Oxygen Species (ROS)
{"title":"Clinical evaluation of a novel gallotannin-enriched Galla Rhois extract (GRE) on vital cognitive functions in healthy volunteers: A randomized, double-blind, placebo-controlled study","authors":"Marvin Heuer, Christopher Baker, Michael Sedlak, Kim Jin Woo, Kim Hyun Kee, Debasis Bagchi","doi":"10.31989/ffhd.v13i10.1213","DOIUrl":"https://doi.org/10.31989/ffhd.v13i10.1213","url":null,"abstract":"Background: Oxidative damage has emerged as one of the significant factors in the onset of neurodegenerative disorders, primarily owing to a) higher usage of oxygen by brain cells and b) higher amounts of lipids in the neuronal membranes. Together, these have resulted in higher susceptibility of brain cells to ROS-induced lipid peroxidation, damage to other cellular macromolecules, including nucleic acids and proteins, and death of neurons by apoptosis, ferroptosis, and necrosis. The design of therapeutics to prevent oxidative damage-induced brain stroke or other irreversible injuries has remained a challenge owing to the continuous production of free radicals at the site of damage. In this respect, plant-derived tannins and tannic acid derivatives have exhibited promising results in scavenging free radicals and inhibiting lipid peroxidation.Objective: This investigation reports the effect of Ghala Rois extract (GRE), a methyl gallate enriched gall formed on the nutgall sumac tree, Rhus javanica L. (Anacardiaceae), on enhancing the cognitive function and overall well-being through a double-blind, placebo-controlled, randomized, parallel-group design study on 80 healthy adult volunteers over a period of six consecutive weeks.Results: Administration of GRE in the dosage of 750 mg/day p.o. (with an equal amount of corn starch as a placebo) after breakfast resulted in significant improvement in cognitive functions as assessed by HappyNeuroPro, a digital therapy tool, and the cognitive tools under Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MOCA) and Adult ADHD Self-Reported Scale (ASRSv1.1). Concomitantly, the GRE-treated subjects also scored significantly higher (p = 0.033) in the roles of Physical and Emotional Problems, Energy/Fatigue, Emotional well-being, and pain as evaluated by linearly independent pairwise comparisons among the estimated marginal means and computed using alpha = 0.05. In the Global Evaluation Questionnaire, over 77% of the treated subjects believed that the product increased their attention span, and helped their memory by 70%, and they also indicated their willingness to buy the commercially available GRE. Evaluation of the complete metabolic profile in the GRE-treated subjects significantly lowered the blood urea nitrogen (BUN) (11.98 mg/dL) as compared to placebo (13.27 mg/dL) (p = 0.011) as well as SGPT/ALT from 28.09 U/L (placebo) to 18.17 U/L (GRE-treated) (p<0.001). However, the vital parameters, including heart rate and systolic/diastolic pressure, were not affected by the consumption of GRE extract.Conclusion: The study demonstrated that GRE could be used as an effective new phytotherapeutic for reversing oxidative damage-induced neuronal degeneration and improving cognitive health in these study individuals.Keywords: Galla Rhois extract (GRE); Rhus javanica L. (family Anacardiaceae); Cognitive functions; ADHD; Executive function; Verbal memory; Safety, Reactive Oxygen Species (ROS)","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135567623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-05DOI: 10.31989/ffhd.v13i10.1211
Armenuhi Moghrovyan, Mikayel Ginovyan, Nikolay Avtandilyan, Lilya Parseghyan, Armen Voskanyan, Naira Sahakyan, Anna Darbinyan
Backgrounds: Plant compounds are of great value due to their analgesic, anti-inflammatory, cytotoxic, and other characteristics. This study aimed to examine the chemical composition and impact of the Origanum vulgare hydro-ethanolic extract on nociceptive behavior and anti-inflammatory properties in in vivo mice models.Methods: Gas chromatography-mass spectrometry and high-performance liquid chromatography techniques were employed to determine the major active phytochemicals of the extract. The effects of the extract on nociceptive reactions were evaluated using formalin and hot plate tests, while the anti-inflammatory effect was determined using the Carrageenan test. The methyl-thiazolyl-tetrazolium assay was applied to analyze growth-inhibiting properties of the extract on MCF-7 breast cancer cells.Results: Various flavonoids and organic acids were identified in O. vulgare hydro-ethanolic extract. The extract possessed low cytotoxicity on MCF-7 cells as only 1 mg/mL or higher concentrations of the extract induced considerable growth inhibition. The formalin and hot plate tests demonstrated some sensitizing effects of the investigated extract (5 mg/kg) on mice. Moreover, the Carrageenan test revealed pronounced anti-inflammatory properties of the O. vulgare extract.Conclusion: The potent biological activity of the wild oregano herb extract makes it a promising source for the development of medicinal preparations. Keywords: Nociceptive behavior, anti-inflammatory effect, Oregano, flavonoids, chemical composition, cytotoxicity, in vivo mice models.
{"title":"The possible anti-inflammatory properties of hydro-ethanolic extract of Oregano","authors":"Armenuhi Moghrovyan, Mikayel Ginovyan, Nikolay Avtandilyan, Lilya Parseghyan, Armen Voskanyan, Naira Sahakyan, Anna Darbinyan","doi":"10.31989/ffhd.v13i10.1211","DOIUrl":"https://doi.org/10.31989/ffhd.v13i10.1211","url":null,"abstract":"Backgrounds: Plant compounds are of great value due to their analgesic, anti-inflammatory, cytotoxic, and other characteristics. This study aimed to examine the chemical composition and impact of the Origanum vulgare hydro-ethanolic extract on nociceptive behavior and anti-inflammatory properties in in vivo mice models.Methods: Gas chromatography-mass spectrometry and high-performance liquid chromatography techniques were employed to determine the major active phytochemicals of the extract. The effects of the extract on nociceptive reactions were evaluated using formalin and hot plate tests, while the anti-inflammatory effect was determined using the Carrageenan test. The methyl-thiazolyl-tetrazolium assay was applied to analyze growth-inhibiting properties of the extract on MCF-7 breast cancer cells.Results: Various flavonoids and organic acids were identified in O. vulgare hydro-ethanolic extract. The extract possessed low cytotoxicity on MCF-7 cells as only 1 mg/mL or higher concentrations of the extract induced considerable growth inhibition. The formalin and hot plate tests demonstrated some sensitizing effects of the investigated extract (5 mg/kg) on mice. Moreover, the Carrageenan test revealed pronounced anti-inflammatory properties of the O. vulgare extract.Conclusion: The potent biological activity of the wild oregano herb extract makes it a promising source for the development of medicinal preparations. Keywords: Nociceptive behavior, anti-inflammatory effect, Oregano, flavonoids, chemical composition, cytotoxicity, in vivo mice models.","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134973532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-04DOI: 10.31989/ffhd.v13i10.1142
Aya Abe, Mahendra P. Kapoor, So Morishima, Derek Timm, Atsushi Nakajima, Makoto Ozeki, Norio Sato
Background: Moringa oleifera is well recognized for its rich nutritional content and diverse bioactive compounds. Moringa, abundant in various bioactive compounds such as flavonoids and alkaloids present across all plant parts including leaves, stems, and roots, is especially rich in glucomoringin within its seeds. The antioxidant activity of moringin has already been verified in numerous cellular and animal experiments.Objective: The study investigates the effects of Moringa seed extract (MSE), abundant in glucomoringin, on fatigue-related physical discomfort and sleep quality, as indicators of quality of life (QOL) in healthy individuals.Methods: A randomized, double-blind, placebo-controlled, parallel-group study was conducted, administering MSE containing 12 mg of glucomoringin or placebo to healthy adult males and females daily for four consecutive weeks. Quality of life (QOL) questionnaire on fatigue, physical discomfort, sleep, and motivation was evaluated using a visual analog scale (VAS) at the start of the study and the end of each subsequent week until week 4. The impact on QOL was assessed through subjective evaluations and oxidative stress markers.Results: As a result, the efficacy of MSE intake in reducing stiff shoulder/neck pain, joint pain, and muscle pain was confirmed. Based on the oxidative stress markers, significant effects were observed among the participants with relatively high oxidative stress. A greater improvement in physical discomfort, reduced fatigue, and enhanced sleep quality were also noticed among female participants with MSE consumption. Conclusion: MSE consumption has showed potential in lowering physical discomfort brought on by exhaustion, promoting overall QOL, and improving sleep quality. (UMIN000049070)Keywords:moringa; moringin; glucomoringin; physical discomfort; sleep quality; fatigue; pain
{"title":"Alleviating the physical discomfort in healthy individuals with Moringa seed extract: a randomized, double-blind, placebo-controlled, parallel-group trial","authors":"Aya Abe, Mahendra P. Kapoor, So Morishima, Derek Timm, Atsushi Nakajima, Makoto Ozeki, Norio Sato","doi":"10.31989/ffhd.v13i10.1142","DOIUrl":"https://doi.org/10.31989/ffhd.v13i10.1142","url":null,"abstract":"Background: Moringa oleifera is well recognized for its rich nutritional content and diverse bioactive compounds. Moringa, abundant in various bioactive compounds such as flavonoids and alkaloids present across all plant parts including leaves, stems, and roots, is especially rich in glucomoringin within its seeds. The antioxidant activity of moringin has already been verified in numerous cellular and animal experiments.Objective: The study investigates the effects of Moringa seed extract (MSE), abundant in glucomoringin, on fatigue-related physical discomfort and sleep quality, as indicators of quality of life (QOL) in healthy individuals.Methods: A randomized, double-blind, placebo-controlled, parallel-group study was conducted, administering MSE containing 12 mg of glucomoringin or placebo to healthy adult males and females daily for four consecutive weeks. Quality of life (QOL) questionnaire on fatigue, physical discomfort, sleep, and motivation was evaluated using a visual analog scale (VAS) at the start of the study and the end of each subsequent week until week 4. The impact on QOL was assessed through subjective evaluations and oxidative stress markers.Results: As a result, the efficacy of MSE intake in reducing stiff shoulder/neck pain, joint pain, and muscle pain was confirmed. Based on the oxidative stress markers, significant effects were observed among the participants with relatively high oxidative stress. A greater improvement in physical discomfort, reduced fatigue, and enhanced sleep quality were also noticed among female participants with MSE consumption. Conclusion: MSE consumption has showed potential in lowering physical discomfort brought on by exhaustion, promoting overall QOL, and improving sleep quality. (UMIN000049070)Keywords:moringa; moringin; glucomoringin; physical discomfort; sleep quality; fatigue; pain","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135590574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-27DOI: 10.31989/ffhd.v13i9.1130
Omowumi Kayode, Nkechinyere J. Ohanaka, Ikeoluwa O. Kolawole, Olatayo A. Afolabi, Matthew E. Iyobhebhe
Functional foods are foods that, in addition to providing basic nutrients, have health-promoting effects when ingested consistently over a period of time. These foods can exist in their natural forms or might have been enhanced through fortification, biotransformation, or genetic modification. Globally, a lot of attention has been drawn to its consumption as a cheaper and safer remedy for the prevention, treatment, and management of several health-related conditions, including those of reproductive difficulties such as infertility. Infertility is a condition that is prevalent among some married couples and involves difficulty in procreation after about twelve months of unprotected intercourse between heterosexual couples. Oxidative stress-related infertility can be managed by functional foods rich in antioxidants and other phytochemicals with modulatory effects on reproductive tissues. Consumption of prescribed functional foods can alleviate functional disorders of the reproductive tissues and hence encourage higher success rates at conception trials.Keywords: functional foods, reproductive, infertility, health, antioxidants
{"title":"Reproductive health-promoting effects of functional foods","authors":"Omowumi Kayode, Nkechinyere J. Ohanaka, Ikeoluwa O. Kolawole, Olatayo A. Afolabi, Matthew E. Iyobhebhe","doi":"10.31989/ffhd.v13i9.1130","DOIUrl":"https://doi.org/10.31989/ffhd.v13i9.1130","url":null,"abstract":"Functional foods are foods that, in addition to providing basic nutrients, have health-promoting effects when ingested consistently over a period of time. These foods can exist in their natural forms or might have been enhanced through fortification, biotransformation, or genetic modification. Globally, a lot of attention has been drawn to its consumption as a cheaper and safer remedy for the prevention, treatment, and management of several health-related conditions, including those of reproductive difficulties such as infertility. Infertility is a condition that is prevalent among some married couples and involves difficulty in procreation after about twelve months of unprotected intercourse between heterosexual couples. Oxidative stress-related infertility can be managed by functional foods rich in antioxidants and other phytochemicals with modulatory effects on reproductive tissues. Consumption of prescribed functional foods can alleviate functional disorders of the reproductive tissues and hence encourage higher success rates at conception trials.Keywords: functional foods, reproductive, infertility, health, antioxidants","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135534753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Mannoproteins (MP) contained in the yeast cell wall (YCW) have high biological activity and possess several techno-functional properties. They are actively used in oenology, as a colloidal stabilizer and an inhibitor of the crystallization of potassium bitartrate, agriculture, as a feed additive for animals, the food industry, as an emulsifier. We obtained MP from Saccharomyces cerevisiae yeast and evaluated antimicrobial properties against Bacillus subtilis and Escherichia coli.Methods: YCW was obtained by autolysis of 15% yeast suspension of S. cerevisiae for 24 h at the temperature of 55° C with the initial pH of 5.0 and mixing at 120 rpm. MP preparations from YCW were obtained in two ways, namely, thermal extraction at the temperature of 121° C and alkaline extraction. The precipitated protein was dried in a dehydrator and then crushed. After that, MP were tested in relation to two cultures of E. coli and B. subtilis by sowing on liquid and solid nutrient media, with the addition of MP preparation obtained by various methods and different concentrations.Results: As a result of induced autolysis, YCW was obtained with a yield of 27%. The MP yield after thermal and alkaline extraction was 12.0 and 12.9%, respectively. In relation to E. coli, alkaline MP showed the inhibitory ability of 37% at the preparation concentration of 5% (m/v). The fraction obtained by thermal means demonstrated minimal antimicrobial activity (AA). Inhibition was 4% relative to the control sample at the preparation concentration of 3% (m/v). According to the experimental data obtained with respect to B. subtilis, AA of the alkaline (3% m/v) fraction was 80%. The thermal (5% m/v) fraction did not manifest AA, on the contrary, it acted as a catalyst for colony growth.Conclusions: Mannoprotein obtained by alkaline extraction showed an inhibitory effect on the growth of B. subtilis and E. coli. The resulting preparation can be used as a feed additive to agriculture animals as an alternative to antibiotics. With proper cleaning, this preparation can be used in medicine and cosmetology. Keywords: mannoprotein, cell wall, yeast, Saccharomyces cerevisiae, antimicrobial properties
{"title":"Obtaining yeast mannoproteins with antimicrobial properties","authors":"Mariia Kuntsova, Tatiana Meledina, Svetlana Davydenko, Dmitrii Manshin, Anastasia Andreeva","doi":"10.31989/ffhd.v13i9.1179","DOIUrl":"https://doi.org/10.31989/ffhd.v13i9.1179","url":null,"abstract":"Introduction: Mannoproteins (MP) contained in the yeast cell wall (YCW) have high biological activity and possess several techno-functional properties. They are actively used in oenology, as a colloidal stabilizer and an inhibitor of the crystallization of potassium bitartrate, agriculture, as a feed additive for animals, the food industry, as an emulsifier. We obtained MP from Saccharomyces cerevisiae yeast and evaluated antimicrobial properties against Bacillus subtilis and Escherichia coli.Methods: YCW was obtained by autolysis of 15% yeast suspension of S. cerevisiae for 24 h at the temperature of 55° C with the initial pH of 5.0 and mixing at 120 rpm. MP preparations from YCW were obtained in two ways, namely, thermal extraction at the temperature of 121° C and alkaline extraction. The precipitated protein was dried in a dehydrator and then crushed. After that, MP were tested in relation to two cultures of E. coli and B. subtilis by sowing on liquid and solid nutrient media, with the addition of MP preparation obtained by various methods and different concentrations.Results: As a result of induced autolysis, YCW was obtained with a yield of 27%. The MP yield after thermal and alkaline extraction was 12.0 and 12.9%, respectively. In relation to E. coli, alkaline MP showed the inhibitory ability of 37% at the preparation concentration of 5% (m/v). The fraction obtained by thermal means demonstrated minimal antimicrobial activity (AA). Inhibition was 4% relative to the control sample at the preparation concentration of 3% (m/v). According to the experimental data obtained with respect to B. subtilis, AA of the alkaline (3% m/v) fraction was 80%. The thermal (5% m/v) fraction did not manifest AA, on the contrary, it acted as a catalyst for colony growth.Conclusions: Mannoprotein obtained by alkaline extraction showed an inhibitory effect on the growth of B. subtilis and E. coli. The resulting preparation can be used as a feed additive to agriculture animals as an alternative to antibiotics. With proper cleaning, this preparation can be used in medicine and cosmetology. Keywords: mannoprotein, cell wall, yeast, Saccharomyces cerevisiae, antimicrobial properties","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135394205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-11DOI: 10.31989/ffhd.v13i9.1228
Sarah Stratton, Danik Martirosyan
Health claims are essential for providing accurate information about the relationship between nutrients and health outcomes to consumers. They facilitate informed decision-making and support individuals in making healthy dietary choices. The U.S. Food and Drug Administration (FDA) regulates health claims to guide consumers in maintaining healthy dietary practices. Health claims on food labels help consumers understand the potential health benefits associated with specific products and make informed choices aligned with their health goals. These claims also promote transparency and trust in the food industry by providing evidence-based information. The process of submitting a health claim to the FDA involves complying with regulatory requirements and providing robust scientific evidence. Scientific evidence plays a crucial role in substantiating health claims and should be based on well-designed clinical trials, epidemiological studies, and mechanistic research. Adhering to FDA labeling requirements ensures the accurate and informative presentation of health claims on food labels. The Functional Food Center (FFC) is working on theoretical aspects of creating ideal functional food (FF) products as well as how these products should be identified with special FF labels. While the FDA focuses on evaluating health claims, the FFC emphasizes the safe and effective use of functional foods. Understanding the FDA's evaluation process and the FFC's guidelines is crucial for researchers, food manufacturers, and policymakers to navigate the regulatory landscape and promote informed consumer choices. Compliance with regulatory guidelines, adherence to scientific standards, and clear communication of health claims contribute to a more informed and health-conscious society.Keywords: Health claims, health outcomes, regulatory requirements, functional foods, health benefits
{"title":"Comparing the FDA's process for health claim submission and the FFC's guidelines for regulating functional foods","authors":"Sarah Stratton, Danik Martirosyan","doi":"10.31989/ffhd.v13i9.1228","DOIUrl":"https://doi.org/10.31989/ffhd.v13i9.1228","url":null,"abstract":"Health claims are essential for providing accurate information about the relationship between nutrients and health outcomes to consumers. They facilitate informed decision-making and support individuals in making healthy dietary choices. The U.S. Food and Drug Administration (FDA) regulates health claims to guide consumers in maintaining healthy dietary practices. Health claims on food labels help consumers understand the potential health benefits associated with specific products and make informed choices aligned with their health goals. These claims also promote transparency and trust in the food industry by providing evidence-based information. The process of submitting a health claim to the FDA involves complying with regulatory requirements and providing robust scientific evidence. Scientific evidence plays a crucial role in substantiating health claims and should be based on well-designed clinical trials, epidemiological studies, and mechanistic research. Adhering to FDA labeling requirements ensures the accurate and informative presentation of health claims on food labels. The Functional Food Center (FFC) is working on theoretical aspects of creating ideal functional food (FF) products as well as how these products should be identified with special FF labels. While the FDA focuses on evaluating health claims, the FFC emphasizes the safe and effective use of functional foods. Understanding the FDA's evaluation process and the FFC's guidelines is crucial for researchers, food manufacturers, and policymakers to navigate the regulatory landscape and promote informed consumer choices. Compliance with regulatory guidelines, adherence to scientific standards, and clear communication of health claims contribute to a more informed and health-conscious society.Keywords: Health claims, health outcomes, regulatory requirements, functional foods, health benefits","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135939231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Amaranthus viridis and Sauropus androgynous are common edible vegetables consumed by the natives of Asia. This research aimed to carry out a comparative evaluation on the antioxidant activity and nitric oxide production effect of crispy vegetables formula of A. viridis and S. androgynous made by thermal processing.Methods: A. viridis and S. androgynous fresh vegetable leaves were dried, processed and formulated into crispy vegetables: A. viridis crispy vegetables, S. androgynous crispy vegetables and 50:50% (w/w) mixture of A. viridis and S. androgynous crispy vegetables. Total phenolic and flavonoids contents of the three crispy vegetables were evaluated using Folin Ciocalteu colorimetric and aluminum chloride assay. Results: S. androgynous crispy vegetable had higher phenolic (36.55 ± 0.01 mg GAE/g) and flavonoid (81.17±0.00 mg QE/g) contents than A. viridis crispy vegetables (16.55±0.00 mg GAE/g and 40.58±0.01 QE/g respectively) and 50:50% mixed crispy vegetable formula of A. viridis and S. androgynous (26.01 ± 0.00 mg GAE/g; 59.38±0.00 QE/g respectively). S. androgynous crispy vegetable sheet also demonstrated a better antioxidant activity against the radical effect of DPPH (15.43±0.07%), ABTS (34.04±0.05%) and FRAP (1.73±0.01) than A. viridis (13.45±0.11%, 26.35±0.17 &; 1.56±0.01 respectively) and was comparable with mixed formula (14.23±0.43%; 32.31±0.12%, 1.81±0.03 respectively). A significant dose-dependent cytotoxic effect was induced by the three crispy vegetables with IC50 of 8.33±0.69, 7.84±0.35, 7.86±0.33 respectively on HUVECs. The results also showed that mixed crispy vegetables (50:50%) promoted endothelial nitric oxide production. Conclusion:The study concludes that the antioxidant activity of the crispy vegetables reflects the total phenolic and total flavonoid content as their functional properties. Our findings also suggest that thermal processing of foods could have significant effect on the antioxidant activity of functional foods; however, A. viridis and S. androgynous prepared in crispy form may pose no potential threat to the body like the fresh vegetables.Keywords:Amaranthus viridis, S. androgynous, crispy vegetables, antioxidant, nitric oxide, hypertension, Thailand
{"title":"Evaluation of the antioxidant activity and nitric oxide production effect of formulated crispy vegetables from thermal processing of Amaranthus viridis and Sauropus androgynous","authors":"Juwainee Madardam, Saowakon Wattanachant, Chutha Takahashi Yupanqui","doi":"10.31989/ffhd.v13i9.1116","DOIUrl":"https://doi.org/10.31989/ffhd.v13i9.1116","url":null,"abstract":"Background: Amaranthus viridis and Sauropus androgynous are common edible vegetables consumed by the natives of Asia. This research aimed to carry out a comparative evaluation on the antioxidant activity and nitric oxide production effect of crispy vegetables formula of A. viridis and S. androgynous made by thermal processing.Methods: A. viridis and S. androgynous fresh vegetable leaves were dried, processed and formulated into crispy vegetables: A. viridis crispy vegetables, S. androgynous crispy vegetables and 50:50% (w/w) mixture of A. viridis and S. androgynous crispy vegetables. Total phenolic and flavonoids contents of the three crispy vegetables were evaluated using Folin Ciocalteu colorimetric and aluminum chloride assay. Results: S. androgynous crispy vegetable had higher phenolic (36.55 ± 0.01 mg GAE/g) and flavonoid (81.17±0.00 mg QE/g) contents than A. viridis crispy vegetables (16.55±0.00 mg GAE/g and 40.58±0.01 QE/g respectively) and 50:50% mixed crispy vegetable formula of A. viridis and S. androgynous (26.01 ± 0.00 mg GAE/g; 59.38±0.00 QE/g respectively). S. androgynous crispy vegetable sheet also demonstrated a better antioxidant activity against the radical effect of DPPH (15.43±0.07%), ABTS (34.04±0.05%) and FRAP (1.73±0.01) than A. viridis (13.45±0.11%, 26.35±0.17 &; 1.56±0.01 respectively) and was comparable with mixed formula (14.23±0.43%; 32.31±0.12%, 1.81±0.03 respectively). A significant dose-dependent cytotoxic effect was induced by the three crispy vegetables with IC50 of 8.33±0.69, 7.84±0.35, 7.86±0.33 respectively on HUVECs. The results also showed that mixed crispy vegetables (50:50%) promoted endothelial nitric oxide production. Conclusion:The study concludes that the antioxidant activity of the crispy vegetables reflects the total phenolic and total flavonoid content as their functional properties. Our findings also suggest that thermal processing of foods could have significant effect on the antioxidant activity of functional foods; however, A. viridis and S. androgynous prepared in crispy form may pose no potential threat to the body like the fresh vegetables.Keywords:Amaranthus viridis, S. androgynous, crispy vegetables, antioxidant, nitric oxide, hypertension, Thailand","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136299877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-31DOI: 10.31989/ffhd.v13i8.1158
A. Tadevosyan, A. Hakobjanyan, A. Tovmasyan, A. Asatryan, H. Roosta, M. Daryadar
Background: Moringa has a hypoglycemic and hypolipidemic influence, and the water extract of its leaves can decrease the amount of glucose during Diabetes Mellitus. Moringa leaves in extract or powder form influenced type I and type II diabetes by enhancing insulin secretion and improving glycogen synthesis and glucose uptake in the liver and muscles. This occurred in the case of type I diabetes, as well as by inhibiting glucose uptake and slowing gastric emptying in the case of type II diabetes.Objective: In our study, we try to evaluate the hypoglycemic, hypolipidemic, and antistress activity of Moringa grown for the first time in Armenia in hydroponic and soil conditions to find out the beneficial cultivation method of Moringa from the point of view of these activities.Materials and Methods: The study was done with the usage of a rabbit model, using water extract of Moringa leaves made with a 150 mg count of leaves on 1 kg weight of the animal. Hyperglycemia in the rabbits was induced by 21-day immobilization stress (3 hours a day). Rabbits were divided into 4 groups: the first group was treated with the extract from the hydroponic Moringa in a dose of 2 ml/day, the second group received the extract from the soil Moringain a dose of 2 ml/day, the third group was the control—which did not receive any extract, but was exposed to stress, like first and second groups— and the fourth group was the norm, which did not receive any extract and was stress free. Each group included 3 animals as means of replication. The glycogen level was measured in the livers, hearts, and muscles of rabbits. The level of glucose, total cholesterol, HDL, and LDL were measured in the serum of animals.Results: Our results showed that after a 21-day period of stress, the increased level of glucose stays at a high level in control groups, while the use of a hydroponic Moringa extract developed stress tolerance in animals and in soil Moringa extract stabilizes glucose levels in the blood. The cholesterol exceeded the norm 1.8 times in the control group and was inferior in hydroponic and soil groups by 1.5 and 1.4 times, respectively. These changes reflect the amount of HDL and LDL. Under stress conditions, the quantity of glycogen decreased from the norm in the liver, muscles, and heart muscle in the control group (2.5; 2.4; and 2.4 times, respectively) and increased in hydroponic (1.6; 1.5; and 2.1 times, respectively) and soil (1.6; 1.5; and 2.4 times, respectively) groups. This evidence shows that under stress conditions the glucose level of blood is improved on the 21st day of stress in groups that received the extract of moringa because of glucose exchange to glycogen with high efficiency in different organs.Keywords: antistress activity, diabetes, HDL, glucose, Moringa oleifera, total cholesterol
{"title":"Hypoglycemic and hypolipidemic activity of moringa grown in hydroponics and soil in Ararat Valley","authors":"A. Tadevosyan, A. Hakobjanyan, A. Tovmasyan, A. Asatryan, H. Roosta, M. Daryadar","doi":"10.31989/ffhd.v13i8.1158","DOIUrl":"https://doi.org/10.31989/ffhd.v13i8.1158","url":null,"abstract":"Background: Moringa has a hypoglycemic and hypolipidemic influence, and the water extract of its leaves can decrease the amount of glucose during Diabetes Mellitus. Moringa leaves in extract or powder form influenced type I and type II diabetes by enhancing insulin secretion and improving glycogen synthesis and glucose uptake in the liver and muscles. This occurred in the case of type I diabetes, as well as by inhibiting glucose uptake and slowing gastric emptying in the case of type II diabetes.Objective: In our study, we try to evaluate the hypoglycemic, hypolipidemic, and antistress activity of Moringa grown for the first time in Armenia in hydroponic and soil conditions to find out the beneficial cultivation method of Moringa from the point of view of these activities.Materials and Methods: The study was done with the usage of a rabbit model, using water extract of Moringa leaves made with a 150 mg count of leaves on 1 kg weight of the animal. Hyperglycemia in the rabbits was induced by 21-day immobilization stress (3 hours a day). Rabbits were divided into 4 groups: the first group was treated with the extract from the hydroponic Moringa in a dose of 2 ml/day, the second group received the extract from the soil Moringain a dose of 2 ml/day, the third group was the control—which did not receive any extract, but was exposed to stress, like first and second groups— and the fourth group was the norm, which did not receive any extract and was stress free. Each group included 3 animals as means of replication. The glycogen level was measured in the livers, hearts, and muscles of rabbits. The level of glucose, total cholesterol, HDL, and LDL were measured in the serum of animals.Results: Our results showed that after a 21-day period of stress, the increased level of glucose stays at a high level in control groups, while the use of a hydroponic Moringa extract developed stress tolerance in animals and in soil Moringa extract stabilizes glucose levels in the blood. The cholesterol exceeded the norm 1.8 times in the control group and was inferior in hydroponic and soil groups by 1.5 and 1.4 times, respectively. These changes reflect the amount of HDL and LDL. Under stress conditions, the quantity of glycogen decreased from the norm in the liver, muscles, and heart muscle in the control group (2.5; 2.4; and 2.4 times, respectively) and increased in hydroponic (1.6; 1.5; and 2.1 times, respectively) and soil (1.6; 1.5; and 2.4 times, respectively) groups. This evidence shows that under stress conditions the glucose level of blood is improved on the 21st day of stress in groups that received the extract of moringa because of glucose exchange to glycogen with high efficiency in different organs.Keywords: antistress activity, diabetes, HDL, glucose, Moringa oleifera, total cholesterol","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43145192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-23DOI: 10.31989/ffhd.v13i8.1147
S. Hadi, D. Shaban, M.A.H. Morgab, Safa Mohammed Jasim
Background: Dates are recognized as a rich source of nutrients, so they are considered a complementary food element positioning them as functional food for athletes. Energy balls are nutritious snack foods that people, individuals and athletes enjoy. They are a source of natural sugars, fibers and proteins that are nutritionally balanced. Food supplements consist of protein, fats, vitamins, minerals and carbohydrates. Objective:Enrichment some food products, especially energy-rich balls for athletes that made from dates as a main component for it. Materials and Methods: Date fruits from five cultivars (Dekel Nour, Barhi, zahdi, khlas, sukari) were collected from the orchards of Baghdad Governorate. The fruits were cleaned and dried in an oven at a temperature of 45 ° C until dryn, then the fruits were ground by means of a grinder. The ground forms were kept in sealed glass containers at a laboratory temperature of 20-25 °C until use.Results: Variability in contents was observed across the different cultivars, with the highest percentage of total sugars, amounting to 86.70%, being recorded in Barhi dates. Conversely, the lowest sugar percentage was found in date meat, measuring at 69.20%. The fruits recorded the highest moisture percentage with a value of 30.01%. The mineral composition showed that P was the predominant mineral, followed in descending order by Mg, Ca, and proteins. The protein content ranged from 2.14 to 3.20%, while crude fat ranged from (0.15 - 0.44) %. Moisture content in date varieties with early ripening time was between low and high values of 10% to 30%. The comprehensive nutritional profile encompassed minerals and numerous vitamins, most notably B vitamins. Carbohydrates constitute 70% of the date’s composition, including fructose and glucose. Additionally, dates are rich in calcium, magnesium, selenium, copper, phosphorous, potassium, zinc, sulfur, cobalt, fluorine, and manganese. Lately, interest in dates has increased because of their health importance, catalyzing the development of food products.Keywords: Dates, active substances, chemical compounds, functional foods, oat, nuts
{"title":"Chemical composition and effective compounds of dates and their use in a snack to give energy to athletes","authors":"S. Hadi, D. Shaban, M.A.H. Morgab, Safa Mohammed Jasim","doi":"10.31989/ffhd.v13i8.1147","DOIUrl":"https://doi.org/10.31989/ffhd.v13i8.1147","url":null,"abstract":"Background: Dates are recognized as a rich source of nutrients, so they are considered a complementary food element positioning them as functional food for athletes. Energy balls are nutritious snack foods that people, individuals and athletes enjoy. They are a source of natural sugars, fibers and proteins that are nutritionally balanced. Food supplements consist of protein, fats, vitamins, minerals and carbohydrates. Objective:Enrichment some food products, especially energy-rich balls for athletes that made from dates as a main component for it. Materials and Methods: Date fruits from five cultivars (Dekel Nour, Barhi, zahdi, khlas, sukari) were collected from the orchards of Baghdad Governorate. The fruits were cleaned and dried in an oven at a temperature of 45 ° C until dryn, then the fruits were ground by means of a grinder. The ground forms were kept in sealed glass containers at a laboratory temperature of 20-25 °C until use.Results: Variability in contents was observed across the different cultivars, with the highest percentage of total sugars, amounting to 86.70%, being recorded in Barhi dates. Conversely, the lowest sugar percentage was found in date meat, measuring at 69.20%. The fruits recorded the highest moisture percentage with a value of 30.01%. The mineral composition showed that P was the predominant mineral, followed in descending order by Mg, Ca, and proteins. The protein content ranged from 2.14 to 3.20%, while crude fat ranged from (0.15 - 0.44) %. Moisture content in date varieties with early ripening time was between low and high values of 10% to 30%. The comprehensive nutritional profile encompassed minerals and numerous vitamins, most notably B vitamins. Carbohydrates constitute 70% of the date’s composition, including fructose and glucose. Additionally, dates are rich in calcium, magnesium, selenium, copper, phosphorous, potassium, zinc, sulfur, cobalt, fluorine, and manganese. Lately, interest in dates has increased because of their health importance, catalyzing the development of food products.Keywords: Dates, active substances, chemical compounds, functional foods, oat, nuts","PeriodicalId":12623,"journal":{"name":"Functional Foods in Health and Disease","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47739541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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