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Endophytic plant growth promoting bacteria from two halophytes improve wheat performance under salt stress. 两种盐生植物内生促生菌对小麦在盐胁迫下的生产性能有促进作用。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-27 eCollection Date: 2026-01-01 DOI: 10.3389/fpls.2026.1658930
Xuemin He, Hongfei Yuan, Yan Li, Chen Yang

Plant growth-promoting endophytes (PGPE) in halophytes have the potential to enhance plant stress resistance and promote growth, demonstrating broad application prospects in agriculture. The culturable microorganisms inhabiting in halophytes and their potential roles in enhancing salt-stress resistance of crops remain limited. This study isolated culturable endophytic bacteria from the roots of two dominant desert halophytes, Haloxylon ammodendron and Halostachys caspica, determined their growth-promoting abilities, and evaluated their capability in improving wheat performance under salt stress. Five saline-alkali tolerant bacterial strains-identified as Priestia endophyticus (S1, Y5), Priestia licheniformis (S2), Streptomyces griseorubens (S7), and Nocardiopsis aegyptia (Y6)-were characterized. These bacterial strains exhibited robust survival in 1.4 mol/L NaCl and high-pH environments (pH > 11.0), while demonstrating multiple growth-promoting traits, including indole-3-acetic acid (IAA) production and inorganic phosphate solubilization. All of the five strains (except for S2) and mixed culture improved the germination potential at 100 mmol/L NaCl. The strains S7, Y5, and mixed culture significantly increased plant height, root length, above ground fresh and dry weight compared to 200 mmol/L NaCl stressed seedlings (200CK)(p < 0.05). Salt stress significantly decreased chlorophyll content by 25.82% and 34.06% under 100 and 200 mmol/L NaCl in comparison to CK. Conversely, PGPE inoculation significantly promoted chlorophyll synthesis of seedlings under salt stress. PGPE inoculation reduced enzyme activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) relative to the salt stressed seedlings. All inoculation treatments significantly decreased SOD activity by 20.2%-34.62%, and POD activity by 30.79%-53.38%, relative to 200CK. These findings demonstrate that these strains isolated from halophytic plants exhibit positive effects in ameliorating salt stress and improving the growth of wheat seedlings, highlighting their potential for enhancing agricultural productivity in saline-alkali soils.

盐生植物中的植物促生长内生菌(Plant growth-promoting endophytes, PGPE)具有增强植物抗逆性和促进生长的潜力,在农业上具有广阔的应用前景。盐生植物中可培养的微生物及其在提高作物抗盐胁迫能力中的潜在作用仍然有限。本研究从两种优势沙漠盐生植物梭梭(Haloxylon ammodendron)和咸菜(Halostachys caspica)的根中分离出可培养的内生细菌,测定了它们的促生长能力,并评价了它们在盐胁迫下改善小麦生产性能的能力。鉴定了5株耐盐碱细菌,分别为内生葡萄球菌(S1, Y5)、地衣葡萄球菌(S2)、灰色链霉菌(S7)和埃及无心菌(Y6)。这些菌株在1.4 mol/L NaCl和高pH环境(pH > - 11.0)中表现出强劲的生存能力,同时表现出多种促进生长的特性,包括吲哚-3-乙酸(IAA)的产生和无机磷酸盐的溶解。在100 mmol/L NaCl条件下,5个菌株(除S2外)和混合培养均能提高发芽率。与200 mmol/L NaCl胁迫幼苗(200CK)相比,菌株S7、Y5和混合培养显著提高了株高、根长、地上鲜重和干重(p < 0.05)。在100和200 mmol/L NaCl胁迫下,叶绿素含量较对照显著降低25.82%和34.06%。相反,接种PGPE显著促进了盐胁迫下幼苗叶绿素合成。与盐胁迫幼苗相比,接种PGPE降低了过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性。与200CK相比,各处理均显著降低SOD活性20.2% ~ 34.62%,POD活性30.79% ~ 53.38%。这些研究结果表明,这些从盐生植物中分离出来的菌株在改善盐胁迫和促进小麦幼苗生长方面具有积极作用,突出了它们在盐碱地提高农业生产力的潜力。
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引用次数: 0
Defining screening criteria and ranking in-row and inter-row cover crops for irrigated vineyards using a hybrid AHP-TOPSIS model. 使用混合AHP-TOPSIS模型确定灌溉葡萄园的筛选标准和行间覆盖作物排序。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-26 eCollection Date: 2025-01-01 DOI: 10.3389/fpls.2025.1695610
Mehdi Sharifi, Abbas Sayyad, Eman El Sayed

Cover crops are increasingly important in sustainable vineyard management, yet species selection remains site-specific and challenging. We evaluated candidates in two commercial organic vineyards in the semi-arid Okanagan Valley, British Columbia-CFF (13-year Merlot) and KOW (10-year Zweigelt)-using a hybrid multicriteria decision analysis (MCDA) [Analytic Hierarchy Process-Technique for Order Preference by Similarity to Ideal Solution (AHP-TOPSIS)]. Nine in-row and 15 inter-row treatments (annuals and perennials) were evaluated in the 2019 growing season. Field measures included biomass, ground cover, interference with the fruiting zone, invasiveness, pest/disease effects, drought and winter tolerance, and traffic tolerance. In both vineyards, risk of invasiveness (~43-60%) and interference with fruiting zones (~30%) were the most influential criteria. The top under-vine annuals were Lens culinaris (spring lentil), Brassica napus cv. Winfred, and Brassica rapa (purple-top turnip) with high relative closeness (RCi = 0.87-0.96). Among perennials, Ladino Trifolium repens cv. Crescendo ranked the highest (RCi ~ 0.84), supporting its use as a durable under-vine cover. For inter-rows, Pisum sativum + Secale cereale (pea-rye) led at CFF (RCi = 0.89) and placed second at KOW, Trifolium incarnatum ranked second at CFF (RCi = 0.83), and Trifolium alexandrinum led at KOW (RCi = 0.94). Other annual clovers were intermediate (RCi = 0.79-0.88), performing best on finer-textured, cooler, moister sites. The leading perennial inter-row mix was Lolium perenne + Raphanus sativus var. longipinnatus (tillage radish; RCi = 0.94), followed by a Festuca spp. mix (RCi = 0.65-0.69). This MCDA delivers clear, literature-aligned rankings and a data-driven framework to guide regionally adapted cover-crop choices and future web-based decision tools.

覆盖作物在可持续葡萄园管理中越来越重要,但品种选择仍然是特定地点和具有挑战性的。我们使用混合多标准决策分析(MCDA)[层次分析法-基于理想解决方案相似性排序偏好技术(AHP-TOPSIS)]对不列颠哥伦比亚省半干旱的奥肯那根谷的两个商业有机葡萄园的候选葡萄进行了评估——cff(13年梅洛)和KOW(10年茨威格尔)。在2019年生长季对9个行内处理和15个行间处理(一年生和多年生)进行了评价。田间测量包括生物量、地被物、对结果区的干扰、入侵性、病虫害影响、耐旱性和冬季耐受性以及交通耐受性。在这两个葡萄园中,入侵风险(~ 43% -60%)和对果区的干扰(~30%)是影响最大的标准。蔓下顶端一年生植物为春扁豆(Lens culinaris)、甘蓝型油菜(Brassica napus)。与紫顶萝卜(Brassica rapa, RCi = 0.87 ~ 0.96)相对接近度较高。在多年生植物中,Ladino Trifolium代表cv。Crescendo排名最高(RCi ~ 0.84),支持其作为耐用藤蔓下盖的使用。在行间,Pisum sativum + Secale cereale(豌豆-黑麦)在CFF处领先(RCi = 0.89),在KOW处排名第二,Trifolium incarnatum在CFF处排名第二(RCi = 0.83), Trifolium alexandrinum在KOW处领先(RCi = 0.94)。其他一年生三叶草是中等的(RCi = 0.79-0.88),在质地较细、较冷、较潮湿的地方表现最好。多年生间作组合以黑麦草+长叶萝卜(RCi = 0.94)居首,其次为羊茅(RCi = 0.65 ~ 0.69)。该MCDA提供了清晰的、与文献一致的排名和数据驱动的框架,以指导区域适应的覆盖作物选择和未来基于网络的决策工具。
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引用次数: 0
A hyperspectral imaging and machine learning approach for rapid and non-invasive diagnosis of cassava bacterial blight. 木薯细菌性枯萎病快速无创诊断的高光谱成像和机器学习方法。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-26 eCollection Date: 2025-01-01 DOI: 10.3389/fpls.2025.1707646
Ian Carlos Bispo Carvalho, Luciellen da Costa Ferreira, Ana Régia de Mendonça Neves, Alice Maria Silva Carvalho, Henrique Póvoa Rodrigues Lima, Maurício Rossato

This study explores the use of hyperspectral imaging (HSI) combined with machine learning to detect physiological alterations in cassava leaves caused by Xanthomonas phaseoli pv. manihotis (Xpm), a bacterial plant disease that causes significant yield losses worldwide. Therefore, the use of hyperspectral images associated with machine learning can provide information rapidly and accurately, aiming to support decision-making. HSI captures spectral data that reflects biochemical changes in infected plant tissues. An image set of cassava healthy and symptomatic leaves (402 and 450, respectively) were imaged using a hyperspectral camera across wavelengths from 400 to 1000 nm, with image calibration and spectral normalization to improve data quality. Spectral parameters, such as mean reflectance and spectral differences (healthy vs. infected), were analyzed. Six machine learning models were tested for classification: Decision Tree (DT), Random Forest (RF), Support Vector Machine (SVM), K-Nearest Neighbors (KNN), Extreme Gradient Boosting (XGBoost), and Multi-Layer Perceptron (MLP). SVM performed best, achieving the highest accuracy (91.41%), followed by MLP (87.89%), XGBoost (79.69%), and RF (77.34%). DT and KNN had the lowest accuracy (71.88% and 70.31%, respectively). The results suggest that HSI, particularly when combined with SVM, offers a rapid and accurate method for diagnosing cassava bacterial blight, with potential for large-scale field applications.

本研究探讨了使用高光谱成像(HSI)结合机器学习来检测由相黄单胞菌pv引起的木薯叶片生理变化。manihotis (Xpm)是一种在世界范围内造成重大产量损失的细菌性植物病害。因此,使用与机器学习相关的高光谱图像可以快速准确地提供信息,旨在支持决策。HSI捕获反映受感染植物组织生化变化的光谱数据。利用高光谱相机对木薯健康叶片和症状叶片(402和450)进行了400 ~ 1000 nm波段的成像,并进行了图像校准和光谱归一化以提高数据质量。分析光谱参数,如平均反射率和光谱差异(健康与感染)。对六种机器学习模型进行了分类测试:决策树(DT)、随机森林(RF)、支持向量机(SVM)、k近邻(KNN)、极端梯度增强(XGBoost)和多层感知器(MLP)。SVM表现最好,准确率最高(91.41%),其次是MLP(87.89%)、XGBoost(79.69%)和RF(77.34%)。DT和KNN的准确率最低,分别为71.88%和70.31%。结果表明,HSI,特别是与支持向量机相结合,提供了一种快速准确的木薯细菌性枯萎病诊断方法,具有大规模田间应用的潜力。
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引用次数: 0
Comparative analysis of the seed microbiome in four major oilseed crops (rapeseed, sunflower, soybean, sesame) reveals host-specific assembly and potential application of seed core microbes. 通过对四种主要油料作物(油菜籽、向日葵、大豆、芝麻)种子微生物组的比较分析,揭示了种子核心微生物的寄主特异性组装及其潜在应用前景。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-26 eCollection Date: 2026-01-01 DOI: 10.3389/fpls.2026.1721916
Yao Yao, Lelin Zhao, Yun Zhang, Ailing Duan, Yuanxue Yang, Aiyu Wang, Chao Xue, Jianhua Zhang, Ming Zhao

The oilseeds are abundant in oils and proteins, and the production of high-quality oilseeds represents a major objective in modern agriculture. However, oilseed production is constrained by biotic and abiotic stresses, resulting in the decreasing in yield and quality. The seed microbiome has been recognized as a critical determinant of plant health. However, its composition and functional roles in various oilseed crops remains poorly explored. In this study, we utilized 16S rRNA gene amplicon sequencing to compare the bacterial component of seed microbiome and predict their metabolic potential in four oil crops (rapeseed, sunflower, sesame, soybean). Our results revealed that the oilseed harbored high diverse of microbes, and the assembly of microbial community was not random but driven by species and cultivar. From the perspective of microbial functions, the lipid metabolism and other secondary metabolites of seed microbes were associated with corresponding metabolic processes in seeds, such as glucosinolate and linoleic acid, reflecting the functional connection between seed metabolites and seed microbes. Furthermore, the core microbiome was obtained among four oilseed groups, consisting of 18 bacterial amplicon sequence variants (ASVs), including putative plant-beneficial resources, such as Sphingomonas. Notably, strain SE-S32 (Sphingomonas endophytica) isolated from rapeseed seed, one of the core microbes, could improve the resistance of various crops, indicating that seed core microbes could serve as a microbial inoculant among multiple crops. These results provide new insights into the correlation between seed microbiome and seed metabolites, establishing a theoretical foundation for developing microbial strategies to improve oilseed quality and plant health.

油籽富含油脂和蛋白质,生产高质量的油籽是现代农业的主要目标。然而,油籽生产受到生物和非生物胁迫的制约,导致产量和质量下降。种子微生物群已被认为是植物健康的关键决定因素。然而,对其组成和在各种油料作物中的功能作用仍知之甚少。在本研究中,我们利用16S rRNA基因扩增子测序比较了4种油料作物(油菜籽、向日葵、芝麻、大豆)种子微生物组的细菌组成,并预测了它们的代谢潜力。结果表明,油籽微生物多样性较高,微生物群落的聚集不是随机的,而是受物种和品种的驱动。从微生物功能的角度来看,种子微生物的脂质代谢等次生代谢产物与种子中相应的代谢过程相关,如硫代葡萄糖苷、亚油酸等,反映了种子代谢物与种子微生物之间的功能联系。此外,在4个油籽组中获得了核心微生物组,由18个细菌扩增子序列变异(asv)组成,包括假定的植物有益资源,如鞘氨单胞菌。值得注意的是,从油菜种子中分离到的核心微生物之一内生鞘单胞菌SE-S32 (Sphingomonas endophytica)可以提高多种作物的抗性,表明种子核心微生物可以作为多种作物的微生物接种剂。这些结果为研究种子微生物组与种子代谢产物的相关性提供了新的思路,为制定微生物策略提高油籽品质和植物健康奠定了理论基础。
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引用次数: 0
A machine learning and remote sensing approach for accurate forest sub-compartment level vegetation cover change monitoring. 基于机器学习和遥感的森林分房级植被覆盖变化精确监测。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-26 eCollection Date: 2026-01-01 DOI: 10.3389/fpls.2026.1741992
Wenjie Zhang, Yingze Tian, Xiaohui Su, Danzi Wu

Introduction: Accurate detection of vegetation cover type changes in forest sub-compartments (FSCs) is essential for supporting informed forest management decisions. Although various forest change detection algorithms have been developed, fine-scaledetection at the FSC level has received limited attention.

Methods: This study addresses this gap by developing an FSC-scale vegetation cover type change detection method that couples spectral and texture information from Sentinel-2 multispectral imagery with forest management planning and design investigation (FMPI) data. Original spectral bands, vegetation indices, and texture features were extracted and used to construct a classification model based on a particle swarm optimization-back propagation neural network (PSO-BPNN). To evaluate performance, the proposed PSO-BPNN method was compared with random forest (RF), support vector machine (SVM), and conventional back-propagation neural network (BPNN) models.

Results: Results indicate that PSO-BPNN consistently outperformed the other algorithms in change detection at the FSC scale. Specifically, the method achieved an overall accuracy of 91% for change identification, with a Kappa coefficient of 0.86. In the validation dataset, it successfully detected approximately 80% of the changed FSCs.

Discussion: The proposed approach offers a robust and accurate solution for fine-scale forest change monitoring, it enhances the scientific basis for sustainable forest resource management.

引言:准确检测森林分区(FSCs)植被覆盖类型变化对于支持明智的森林管理决策至关重要。尽管已经开发了各种森林变化检测算法,但FSC级别的精细尺度检测受到的关注有限。方法:本研究通过开发一种fsc尺度植被覆盖类型变化检测方法,将Sentinel-2多光谱图像的光谱和纹理信息与森林管理规划设计调查(FMPI)数据相结合,解决了这一空白。提取原始光谱带、植被指数和纹理特征,构建基于粒子群优化-反向传播神经网络(PSO-BPNN)的分类模型。为了评估PSO-BPNN方法的性能,将其与随机森林(RF)、支持向量机(SVM)和传统的反向传播神经网络(BPNN)模型进行了比较。结果:结果表明,PSO-BPNN在FSC尺度下的变化检测中始终优于其他算法。具体而言,该方法对变化识别的总体准确率为91%,Kappa系数为0.86。在验证数据集中,它成功地检测到大约80%的改变的fsc。讨论:该方法为精细尺度森林变化监测提供了稳健、准确的解决方案,增强了森林资源可持续管理的科学依据。
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引用次数: 0
Characterization of the glucosylated anthocyanin profile of 27 red grape (Vitis vinifera L.) varieties grown in Portugal: insights for climate change adaptation. 在葡萄牙种植的27种红葡萄(Vitis vinifera L.)品种的糖基化花青素特征:对气候变化适应的见解。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-26 eCollection Date: 2025-01-01 DOI: 10.3389/fpls.2025.1728700
Miguel Baltazar, Eliana Monteiro, Sandra Pereira, Márcia Carvalho, Elisete Correia, Helena Ferreira, Vânia Silva, Joana Valente, Fernando Alves, Isaura Castro, Berta Gonçalves

Introduction: Climate change poses significant challenges to viticulture, increasing the need for sustainable adaptation strategies such as the identification of resilient Vitis vinifera L. varieties.

Methods: This study characterized the anthocyanin content, profile, and color parameters of 27 red grape varieties cultivated under the same terroir in the Douro Demarcated Region over two consecutive years. Berry biochemical analyses, including chromatographic and colorimetric techniques, alongside gene expression of the anthocyanin biosynthesis genes MybA1, UFGT, and OMT, were conducted to assess varietal and annual variability.

Results: Total anthocyanin content varied significantly among varieties, ranging from 0.14 mg malvidin-3-O-glucoside equivalents per g of dry weight (mg M3G·g-1 DW) in 'Bastardo' to 8.63 mg M3G·g-1 DW in 'Vinhão'. While most varieties demonstrated increased anthocyanin content in the warmer and drier 2022 season, such as 'Tinto Cão' and 'Touriga Franca'; a few displayed notable declines, notably 'Vinhão', highlighting differential responses to abiotic stress. Anthocyanin profiles were dominated by malvidin derivatives, which correlated with enhanced color stability. Nonetheless, cyanidin-3-O-glucoside increased in 2022 in some varieties, while delphinidin and petunidin-3-O-glucosides decreased. CIELAB parameters indicated darker and higher color saturation in berries in 2022, being associated with increases in total anthocyanin content and malvidin derived compounds. Gene expression analysis of MybA1, UFGT, and OMT in six varieties revealed different behaviors.

Discussion: Among all varieties under study, stable anthocyanin profiles across years were observed which could suggest increased resilience potential. These findings highlight the interplay between genetic and environmental factors in shaping anthocyanin dynamics, supporting the use of varietal selection as an adaptation strategy to optimize quality, resilience, and sustainability in wine regions under climate change.

气候变化给葡萄栽培带来了重大挑战,增加了对可持续适应策略的需求,例如鉴定有弹性的葡萄品种。方法:对连续两年在斗罗界域同一风土条件下栽培的27个红葡萄品种的花青素含量、剖面及颜色参数进行了研究。浆果生化分析,包括色谱和比色技术,以及花青素生物合成基因MybA1、UFGT和OMT的基因表达,进行了评估品种和年度变化。结果:品种间总花青素含量差异显著,‘Bastardo’为0.14 mg malvidin-3-O-glucoside equivalents / g干重(mg M3G·g-1 DW),‘vinh o’为8.63 mg M3G·g-1 DW。虽然大多数品种在2022年温暖和干燥的季节显示花青素含量增加,如‘Tinto c ’和‘Touriga Franca’;少数表现出明显的下降,特别是“vinh”,突出了对非生物胁迫的不同反应。花青素谱主要由malvidin衍生物组成,与颜色稳定性增强相关。尽管如此,某些品种的花青素-3- o -葡萄糖苷在2022年有所增加,而飞燕草苷和矮马甲素-3- o -葡萄糖苷则有所减少。CIELAB参数显示,2022年浆果的颜色饱和度更高,颜色更深,这与总花青素含量和malvidin衍生化合物的增加有关。MybA1、UFGT和OMT在6个品种中的基因表达分析显示出不同的行为。讨论:在研究的所有品种中,观察到多年来稳定的花青素谱,这可能表明抗灾潜力增加。这些发现强调了遗传和环境因素在形成花青素动态过程中的相互作用,支持将品种选择作为一种适应策略来优化气候变化下葡萄酒产区的质量、恢复力和可持续性。
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引用次数: 0
Utilization of the litchi male-sterile line 'MS1'-assisting in the selection of suitable harvesting periods for pollen donors for hybridization. 荔枝雄性不育系MS1的利用——辅助杂交花粉供体采收期的选择。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-26 eCollection Date: 2026-01-01 DOI: 10.3389/fpls.2026.1685024
Zhenxuan Quan, Tianjie Deng, Yaoyao Li, Xuewen Zheng, Yi Wang, Weicai Li

Litchi (Litchi chinensis Sonn.), an important fruit originating in China, has received increasing attention for improving breeding efficiency in recent years due to the growing adoption of hybridization. It is a monoecious plant and predominantly relies on cross-pollination for seed production. Thus, enhancing pollen quality is essential for fruit set, which is closely associated with the developmental stages of male flowers. In this study, anthers of the litchi cv. 'D13' at five late developmental stages (half-exposed stage, fully-exposed stage, filament-elongated stage, anthesis, and browning stage) were investigated using paraffin sectioning, staining, in vitro germination, and controlled pollination techniques. Cytological characteristics of pollen were evaluated using paraffin sectioning, while pollen viability was assessed by I2-KI staining, and the germination rate was determined through in vitro culture on sucrose media. Furthermore, pollen collected from these five stages was used for controlled pollination of the male-sterile line 'MS1'. The results indicated that pollen collected at the filament-elongated stage and anthesis exhibited higher viability and germination potential, which resulted in improved fruit set in the male-sterile line 'MS1'. The study identified the filament-elongated stage and anthesis as optimal stages for pollen collection in litchi.

荔枝(Litchi chinensis Sonn.)是一种重要的原产于中国的水果,近年来由于杂交技术的日益普及,荔枝在提高育种效率方面受到越来越多的关注。它是一种雌雄同株植物,主要依靠异花授粉来生产种子。因此,提高花粉质量对坐果至关重要,而坐果质量与雄花发育阶段密切相关。本研究对荔枝花药进行了研究。采用石蜡切片、染色、离体萌发和控制授粉技术研究了‘D13’的5个发育后期(半暴露期、全暴露期、花丝拉长期、开花期和褐变期)。石蜡切片法测定花粉细胞学特征,I2-KI染色法测定花粉活力,蔗糖培养基离体培养法测定发芽率。此外,还将这5个时期采集的花粉用于雄性不育系MS1的控制授粉。结果表明,雄不育系MS1在花期和花期采集的花粉具有较高的活力和萌发潜力,从而提高了结实性。本研究确定了荔枝花丝伸长期和开花期是采集花粉的最佳时期。
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引用次数: 0
Deciphering the profiles of grapevine microbiomes from rhizosphere-to-leaf compartments using multi-omic analysis. 利用多组学分析从根际到叶区室的葡萄微生物组图谱。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-26 eCollection Date: 2025-01-01 DOI: 10.3389/fpls.2025.1734057
Bo Wang, Zhuangwei Wang, Zhenxiao Chen, Jing Zhang, Xicheng Wang

Introduction: Root- and leaf-associated microbiomes are crucial for plant health and influence the yield and quality of the products. The composition of microbes and their association with the host depend on different factors that must be continuously investigated.

Results: We examined the composition and structure of bacterial and fungal communities in four compartments (root, rhizoplane, rhizosphere, and leaf endosphere) of two grapevine varieties ('Alachua' and 'Noble') targeting the 16S rRNA V5-V7 and ITS regions.

Results and discussion: A comparison of the effects of the varieties and compartments showed that they were the major factors contributing to variations in the microbial structures. Bacterial alpha diversity significantly decreased from the rhizosphere to leaf endosphere, while the fungal alpha diversity did not show linear variations. According to normalized stochastic ratio analysis, deterministic processes dominated the bacterial and fungal assemblies of the leaf endosphere while stochastic processes in the rhizosphere and rhizoplane dominated the microbial assemblies. Assembly processes in bacterial and fungal roots differed (stochastic processes in bacteria and deterministic processes in fungi). Twenty shared core operational taxonomic units (OTUs) (bacteria, 13; fungi, 7) were identified across all compartments. Various stilbene compounds in leaf were significantly correlated with these shared core microbes, and weighted gene co-expression network analysis revealed that some hub genes were correlated with these metabolites. Thus, their role as regulators of grapevine microbiome interactions needs to be further evaluated. This study provided new profiles of the microbiota in different grapevines compartments, which suggested their association with grape metabolites and plant genes, representing a major development for further studies focused in understanding the role of these microorganisms for grapevine production.

根和叶相关微生物组对植物健康至关重要,影响产品的产量和质量。微生物的组成及其与宿主的关系取决于必须不断研究的不同因素。结果:研究了两个葡萄品种(Alachua和Noble)针对16S rRNA V5-V7和ITS区域的四个区室(根、根面、根际和叶内圈)细菌和真菌群落的组成和结构。结果与讨论:品种和室的影响比较表明,它们是造成微生物结构变化的主要因素。从根际到叶内圈,细菌α多样性显著降低,真菌α多样性不呈线性变化。归一化随机比值分析表明,叶片内圈的细菌和真菌组合以确定性过程为主,而根际和根际的微生物组合以随机过程为主。细菌和真菌根的组装过程不同(细菌的随机过程和真菌的确定性过程)。共鉴定出20个核心操作分类单元(otu)(细菌13个,真菌7个)。叶片中各种苯乙烯类化合物与这些共享的核心微生物具有显著的相关性,加权基因共表达网络分析显示,一些中心基因与这些代谢物相关。因此,它们作为葡萄藤微生物组相互作用的调节剂的作用需要进一步评估。该研究提供了葡萄不同隔室中微生物群的新概况,表明它们与葡萄代谢物和植物基因有关,为进一步研究这些微生物在葡萄生产中的作用提供了重要的进展。
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引用次数: 0
Integrated transcriptome and metabolome analysis reveal the sesquiterpenoid biosynthesis mechanism of Atractylodes chinensis under drought stress. 综合转录组和代谢组分析揭示了白术在干旱胁迫下倍半萜类化合物的生物合成机制。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-26 eCollection Date: 2025-01-01 DOI: 10.3389/fpls.2025.1751860
Ying Wu, Yao Lu, Qiuju Ye, Bingqian Jin, Boqian Jiang, Dan Wang, Haibo Yin

Introduction: As a traditional Chinese medicine abundant in sesquiterpenoids, Atractylodes chinensis has various pharmacological activities. Nevertheless, the synthesis and metabolism mechanisms of its sesquiterpenoids under drought stress are still not fully elucidated.

Methods: Therefore, this study investigated changes in sesquiterpenoid component contents, gene expression profiles, and metabolite accumulation of A. chinensis under drought stress.

Results: Results showed that the moderate drought stress (MDS) significantly increased the contents of atractylodin, β-eudesmol, and atractylenolide I. Compared with the control group (CK), 10,528, 9,755, and 10,562 differentially expressed genes (DEGs) were identified in the light drought stress (LDS), MDS, and severe drought stress (SDS) groups, respectively. These DEGs are involved in plant-pathogen interaction, plant hormone signal transduction, plant MAPK signal transduction, and starch and sucrose metabolism. Metabolic analysis detected 2,101, 2,112, and 2,144 differentially accumulated metabolites (DAMs) in the LDS, MDS, and SDS groups, including atractylodin, β-eudesmol, and atractylenolide I. These DAMs are primarily enriched in three pathways: "ABC transporters", "D-amino acid metabolism", and "aminoacyl-tRNA biosynthesis". Furthermore, we screened and characterized the expression patterns of DEGs and accumulation levels of DAMs involved in the sesquiterpenoid biosynthesis pathway. Notably, the genes TRINITY_DN12874_c1_g1, TRINITY_DN114406_c0_g1, TRINITY_DN2331_c0_g2, TRINITY_DN7401_c0_g1, TRINITY_DN11676_c0_g1, along with the compound Germacra-1(10),4,11(13)-trien-12-ol, are speculated to be key genes and critical metabolite responding to drought stress, respectively.

Discussion: These findings enhance our understanding of the mechanisms by which drought stress modulates the sesquiterpenoid biosynthesis pathways in A. chinensis.

摘要:白术是一种富含倍半萜类化合物的中药,具有多种药理活性。然而,其倍半萜类化合物在干旱胁迫下的合成和代谢机制仍未完全阐明。方法:研究干旱胁迫下中国冬青倍半萜类成分含量、基因表达谱和代谢物积累的变化。结果:中度干旱胁迫(MDS)显著提高了白术素、β-苦叶酚和白术内酯i的含量。与对照组(CK)相比,轻度干旱胁迫(LDS)组、MDS组和重度干旱胁迫(SDS)组分别鉴定出10,528个、9,755个和10,562个差异表达基因(DEGs)。这些deg参与植物与病原体的相互作用、植物激素信号转导、植物MAPK信号转导以及淀粉和蔗糖代谢。代谢分析在LDS、MDS和SDS组中检测到2,101、2,112和2,144个差异积累代谢物(DAMs),包括苍术素、β-苦叶酚和苍术烯内酯i。这些DAMs主要富集于三个途径:“ABC转运体”、“d -氨基酸代谢”和“氨基酰基- trna生物合成”。此外,我们筛选并表征了deg的表达模式和参与倍半萜类生物合成途径的dam的积累水平。值得注意的是,基因TRINITY_DN12874_c1_g1、TRINITY_DN114406_c0_g1、TRINITY_DN2331_c0_g2、TRINITY_DN7401_c0_g1、TRINITY_DN11676_c0_g1以及化合物Germacra-1(10)、4、11(13)- trien12 -ol被推测分别是响应干旱胁迫的关键基因和关键代谢产物。讨论:这些发现增强了我们对干旱胁迫调节中国金针叶倍半萜类生物合成途径的机制的理解。
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引用次数: 0
Editorial: Utilizing advanced genomics and biochemical tools to strengthen crop adaptation for biotic and abiotic stresses. 社论:利用先进的基因组学和生化工具加强作物对生物和非生物胁迫的适应。
IF 4.1 2区 生物学 Q1 PLANT SCIENCES Pub Date : 2026-01-26 eCollection Date: 2025-01-01 DOI: 10.3389/fpls.2025.1766137
Gurjeet Singh, Santosh Gudi, Simardeep Kaur, Diaa Abd El Moneim
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引用次数: 0
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Frontiers in Plant Science
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