Pub Date : 2023-06-14DOI: 10.1080/01490451.2023.2218377
Anushka Paul, Sudeshna Dey, D. Ram, A. Das
{"title":"Hexavalent Chromium Pollution and its Sustainable Management through Bioremediation","authors":"Anushka Paul, Sudeshna Dey, D. Ram, A. Das","doi":"10.1080/01490451.2023.2218377","DOIUrl":"https://doi.org/10.1080/01490451.2023.2218377","url":null,"abstract":"","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44465875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-09DOI: 10.1080/01490451.2023.2219261
Wenxing Wang, Weiyu Huang, Xiufeng Zhang, Wancheng Zhu, Shihao Guo, Ang Li
Abstract Exogenous microorganisms are introduced into the shale gas reservoir during hydraulic fracturing, but the microbial-mediated changes in the process are yet unclear. Here, a brine–shale–microorganism interaction was simulated in the laboratory for 150 days. Biogenic methane was generated rapidly in 0–16 days, then slowly in 16–40 days and stopped after 40 days, accompanying by brine alkalization. Formation trend of biogenic methane may be independent of the substrate type under the same conditions. Biofilms accelerated mineral dissolution and facilitated secondary mineral formation. Microbial action enhanced the complexity and heterogeneity of macropore structure. Archaeal community structure changed little, while bacterial community structure altered significantly, with bacterial diversity decreasing. Predominant Proteobacteria in the early stage almost disappeared, while Firmicutes, Bacteroides and Desulfobacterota gradually increased. Firmicutes showed a transfer tendency from brine to biofilms. Proteiniphilum and Petrimonas were mainly distributed in brine, whereas Sedimentibacter and Clostridium_sensu_stricto_13 dominated in biofilms. Desulfovibrio was highly distributed in both brine and biofilms. Loss of microbial diversity and the self-assembly of symbiotic microorganisms with cooperation and competition occurred in response to the changes in the shale environment, which also affected methane production, brine pH, and both mineral composition and pore structure.
{"title":"Microbial-mediated Changes during the Brine–Shale–Microorganism Interaction Process","authors":"Wenxing Wang, Weiyu Huang, Xiufeng Zhang, Wancheng Zhu, Shihao Guo, Ang Li","doi":"10.1080/01490451.2023.2219261","DOIUrl":"https://doi.org/10.1080/01490451.2023.2219261","url":null,"abstract":"Abstract Exogenous microorganisms are introduced into the shale gas reservoir during hydraulic fracturing, but the microbial-mediated changes in the process are yet unclear. Here, a brine–shale–microorganism interaction was simulated in the laboratory for 150 days. Biogenic methane was generated rapidly in 0–16 days, then slowly in 16–40 days and stopped after 40 days, accompanying by brine alkalization. Formation trend of biogenic methane may be independent of the substrate type under the same conditions. Biofilms accelerated mineral dissolution and facilitated secondary mineral formation. Microbial action enhanced the complexity and heterogeneity of macropore structure. Archaeal community structure changed little, while bacterial community structure altered significantly, with bacterial diversity decreasing. Predominant Proteobacteria in the early stage almost disappeared, while Firmicutes, Bacteroides and Desulfobacterota gradually increased. Firmicutes showed a transfer tendency from brine to biofilms. Proteiniphilum and Petrimonas were mainly distributed in brine, whereas Sedimentibacter and Clostridium_sensu_stricto_13 dominated in biofilms. Desulfovibrio was highly distributed in both brine and biofilms. Loss of microbial diversity and the self-assembly of symbiotic microorganisms with cooperation and competition occurred in response to the changes in the shale environment, which also affected methane production, brine pH, and both mineral composition and pore structure.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"619 - 631"},"PeriodicalIF":2.3,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42132785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Utilization of organic wastewater for hydrogen production has dual advantages of clean energy generation and bioremediation, which is sustainable for a longer period. To maximize the energy recovery from starch rich wastewater, a two stage system comprising of thermophilic dark fermentation coupled with microbial fuel cell was employed. A single parameter optimization strategy was implemented for the operation of the batch system. The maximum cumulative hydrogen production obtained was 2.56 L L−1 with a 48% reduction in COD under the optimal conditions of 35 g L−1 initial substrate concentration (COD), temperature 60 °C, and pH 6.5. The H2 yield and H2 production rate were 6.8 mol H2/kg CODreduced and 731.3 mL L−1 h−1, respectively. The effect of the organic loading rate (OLR) on H2 production rate was studied in a continuous stirred tank reactor (CSTR). A maximum hydrogen production rate of 913 mL L−1 h−1 was observed at an OLR of 5.6 g L−1 h−1. Effluent recycle played an important role in the improvement of H2 production. A maximum H2 production rate of 1224 mL L−1 h−1 was observed at a recycle ratio of 0.6. Power density of 4.2 W m−3 was observed with MFC using the dark fermentative spent media neutralized with carbonate buffer at an optimal pH of 7. A total COD reduction of 86% was observed.
利用有机废水制氢具有产生清洁能源和生物修复的双重优势，可持续时间更长。为了最大限度地从富含淀粉的废水中回收能量，采用了一种由嗜热暗发酵和微生物燃料电池组成的两级系统。采用单参数优化策略对批处理系统进行了优化。在初始底物浓度(COD)为35 g L−1、温度为60℃、pH为6.5的最佳条件下，最大累积产氢量为2.56 L L−1,COD降低48%。H2产率和H2产率分别为6.8 mol H2/kg coreduced和731.3 mL L−1 h−1。在连续搅拌槽式反应器(CSTR)中研究了有机负载率(OLR)对产氢率的影响。在OLR为5.6 g L−1 h−1时，最大产氢率为913 mL L−1 h−1。出水回用对提高产氢率有重要作用。在循环倍率为0.6的条件下，最大产氢率为1224 mL L−1 h−1。利用碳酸盐缓冲液中和的深色发酵废培养基，在最佳pH为7时，MFC观察到功率密度为4.2 W m−3。总COD降低了86%。
{"title":"Maximization of Energy Recovery from Starch Processing Wastewater by Thermophilic Dark Fermentation Coupled with Microbial fuel Cell Technology","authors":"Mohit Kumar, Soumya Pandit, Vinay Patel, Namita Khanna, Moupriya Nag, Dibyajit Lahiri, Rina Rani Ray, Alok Das, Debabrata Das","doi":"10.1080/01490451.2023.2209555","DOIUrl":"https://doi.org/10.1080/01490451.2023.2209555","url":null,"abstract":"Utilization of organic wastewater for hydrogen production has dual advantages of clean energy generation and bioremediation, which is sustainable for a longer period. To maximize the energy recovery from starch rich wastewater, a two stage system comprising of thermophilic dark fermentation coupled with microbial fuel cell was employed. A single parameter optimization strategy was implemented for the operation of the batch system. The maximum cumulative hydrogen production obtained was 2.56 L L−1 with a 48% reduction in COD under the optimal conditions of 35 g L−1 initial substrate concentration (COD), temperature 60 °C, and pH 6.5. The H2 yield and H2 production rate were 6.8 mol H2/kg CODreduced and 731.3 mL L−1 h−1, respectively. The effect of the organic loading rate (OLR) on H2 production rate was studied in a continuous stirred tank reactor (CSTR). A maximum hydrogen production rate of 913 mL L−1 h−1 was observed at an OLR of 5.6 g L−1 h−1. Effluent recycle played an important role in the improvement of H2 production. A maximum H2 production rate of 1224 mL L−1 h−1 was observed at a recycle ratio of 0.6. Power density of 4.2 W m−3 was observed with MFC using the dark fermentative spent media neutralized with carbonate buffer at an optimal pH of 7. A total COD reduction of 86% was observed.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135159372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-05DOI: 10.1080/01490451.2023.2218362
Abhaya Dayini Behera, Neha Chittoria, S. Kumari, S. Chatterjee, Surajit Das
{"title":"Homology Modeling of Laccase Enzyme of Filamentous Fungus Trichoderma sp. CNSC-2 and Its Role in Pyrene Degradation","authors":"Abhaya Dayini Behera, Neha Chittoria, S. Kumari, S. Chatterjee, Surajit Das","doi":"10.1080/01490451.2023.2218362","DOIUrl":"https://doi.org/10.1080/01490451.2023.2218362","url":null,"abstract":"","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42475057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1080/01490451.2023.2218363
Shelly Verma, A. Daverey
{"title":"Valorization of Dishwashing Scrubber as Biocarrier for the Enrichment of Anammox Bacteria Under Realistic Conditions","authors":"Shelly Verma, A. Daverey","doi":"10.1080/01490451.2023.2218363","DOIUrl":"https://doi.org/10.1080/01490451.2023.2218363","url":null,"abstract":"","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47985914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1080/01490451.2023.2218371
Shahla Soltani-Nezhad
Abstract Heavy metal contaminated sites are the potential source of metal resistance microorganisms. The major focus of this study is to isolate and identify copper ions resistant Pseudomonas from the soil samples collected from mining and refining sites of Sarcheshmeh copper mine in the Kerman Province of Iran and investigate on possible mechanisms for copper resistance. Isolates were selected based on high level of copper ions resistance. The screened isolate was identified as Pseudomonas putida Cu-2 by morphological, biochemical and 16S rDNA gene sequencing analysis. A high degree of copper ions resistance associated with multiple antibiotic resistances was also detected in the screened isolate. The growth pattern of screened isolate with all the studied Cu concentrations was similar to that of control (without Cu ions) indicating that Cu ions would not affect the growth of isolated strain. A decrease in the amount of exopolysaccharides (EPS) was detected after Cu ions – P. Putida Cu-2 culture supernatant interaction. The Fourier transform infrared spectroscopy (FT-IR) peaks for the EPS extracted from the bacterial culture supernatant and the interacted Cu ions were nearly similar. The EPS covering of the Cu ions was established by FT-IR. The study of bacterial EPS covered Cu ions with E. coli PTCC 1338 and S. aureus PTCC 1113 exhibited less toxicity compared to uncoated Cu ions. The presence of copA gene that encodes for the multi-copper oxidase was investigated in the selected strain. The present study suggests that the capping of Cu ions by bacterially produced EPS and the presence of copA gene serve as the probable mechanisms of copper resistance.
{"title":"Study of Copper Resistant Pseudomonas Strains Isolated from Soil in Sarcheshmeh Copper Mine, in Iran and Investigation of the Mechanisms Involved in Copper Resistance","authors":"Shahla Soltani-Nezhad","doi":"10.1080/01490451.2023.2218371","DOIUrl":"https://doi.org/10.1080/01490451.2023.2218371","url":null,"abstract":"Abstract Heavy metal contaminated sites are the potential source of metal resistance microorganisms. The major focus of this study is to isolate and identify copper ions resistant Pseudomonas from the soil samples collected from mining and refining sites of Sarcheshmeh copper mine in the Kerman Province of Iran and investigate on possible mechanisms for copper resistance. Isolates were selected based on high level of copper ions resistance. The screened isolate was identified as Pseudomonas putida Cu-2 by morphological, biochemical and 16S rDNA gene sequencing analysis. A high degree of copper ions resistance associated with multiple antibiotic resistances was also detected in the screened isolate. The growth pattern of screened isolate with all the studied Cu concentrations was similar to that of control (without Cu ions) indicating that Cu ions would not affect the growth of isolated strain. A decrease in the amount of exopolysaccharides (EPS) was detected after Cu ions – P. Putida Cu-2 culture supernatant interaction. The Fourier transform infrared spectroscopy (FT-IR) peaks for the EPS extracted from the bacterial culture supernatant and the interacted Cu ions were nearly similar. The EPS covering of the Cu ions was established by FT-IR. The study of bacterial EPS covered Cu ions with E. coli PTCC 1338 and S. aureus PTCC 1113 exhibited less toxicity compared to uncoated Cu ions. The presence of copA gene that encodes for the multi-copper oxidase was investigated in the selected strain. The present study suggests that the capping of Cu ions by bacterially produced EPS and the presence of copA gene serve as the probable mechanisms of copper resistance.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"582 - 589"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43820056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1080/01490451.2023.2218839
El Houcine Ait-Ouakrim, A. Chakhchar, C. El Modafar, A. Douira, S. Amir, S. Ibnsouda-Koraichi, B. Belkadi, A. Filali-Maltouf
Abstract One of the most restricting macronutrients for crop yield worldwide is phosphorus, which is often less than 1% of the total amount contained in soils. To promote its bioavailability, there are microorganisms considered vital that can solubilize and mineralize their pools in soils. In the current study, we assessed the potential of phosphate-solubilizing bacteria (PSB), isolated from the olive tree rhizosphere grown on phosphate sludge, to solubilize phosphate, promote plant growth, and tolerate abiotic stresses. Based on 16S rRNA gene sequencing, twenty-four PSB strains were identified and retained for in vitro analysis. According to the results, all the strains were able to solubilize the Moroccan rock phosphate, with soluble phosphate concentrations ranged from 5.34 µg/mL to 227 µg/mL. Furthermore, the majority of the strains are thermo-tolerant and halotolerant. Nonetheless, only five strains produced indole acetic acid. Regarding biocontrol potentialities, several PSB strains were characterized by producing hydrogen cyanide and hydrolytic enzymes (cellulase, and chitinase), of which three strains identified as Pseudomonas moraviensis, Bacillus cereus, and Bacillus aryabhattai, with a remarkable multi-trait combination were selected for antagonism and co-inoculation tests. The findings revealed that these PSB strains significantly inhibited Fusarium oxysporum (17.65%–62.35%) and Verticillium dahliae (52.35%–66.87%) and promoted common bean growth. The consortium of the three strains showed the best results by significantly increasing both plant height and tap root length and dry biomass compared to individual inoculation. The PSB selected from the olive tree rhizosphere growing on phosphate sludge have the potential to be useful as biofertilizer and biocontrol agents for attaining sustainable food crop production.
{"title":"Assessment of Potent Phosphate-Solubilizing Bacteria Isolated from the Olive Tree Rhizosphere Grown on Phosphate Sludge and Their Effect on Common Bean Growth","authors":"El Houcine Ait-Ouakrim, A. Chakhchar, C. El Modafar, A. Douira, S. Amir, S. Ibnsouda-Koraichi, B. Belkadi, A. Filali-Maltouf","doi":"10.1080/01490451.2023.2218839","DOIUrl":"https://doi.org/10.1080/01490451.2023.2218839","url":null,"abstract":"Abstract One of the most restricting macronutrients for crop yield worldwide is phosphorus, which is often less than 1% of the total amount contained in soils. To promote its bioavailability, there are microorganisms considered vital that can solubilize and mineralize their pools in soils. In the current study, we assessed the potential of phosphate-solubilizing bacteria (PSB), isolated from the olive tree rhizosphere grown on phosphate sludge, to solubilize phosphate, promote plant growth, and tolerate abiotic stresses. Based on 16S rRNA gene sequencing, twenty-four PSB strains were identified and retained for in vitro analysis. According to the results, all the strains were able to solubilize the Moroccan rock phosphate, with soluble phosphate concentrations ranged from 5.34 µg/mL to 227 µg/mL. Furthermore, the majority of the strains are thermo-tolerant and halotolerant. Nonetheless, only five strains produced indole acetic acid. Regarding biocontrol potentialities, several PSB strains were characterized by producing hydrogen cyanide and hydrolytic enzymes (cellulase, and chitinase), of which three strains identified as Pseudomonas moraviensis, Bacillus cereus, and Bacillus aryabhattai, with a remarkable multi-trait combination were selected for antagonism and co-inoculation tests. The findings revealed that these PSB strains significantly inhibited Fusarium oxysporum (17.65%–62.35%) and Verticillium dahliae (52.35%–66.87%) and promoted common bean growth. The consortium of the three strains showed the best results by significantly increasing both plant height and tap root length and dry biomass compared to individual inoculation. The PSB selected from the olive tree rhizosphere growing on phosphate sludge have the potential to be useful as biofertilizer and biocontrol agents for attaining sustainable food crop production.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"605 - 617"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48583480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1080/01490451.2023.2218383
S. Gohel, V. Majithiya, Satya P. Singh
Abstract Saline and alkaline habitats of the Okha coastline, Gujarat, India, were explored for the diversity of actinobacteria. Based on the phenotypic and genotypic heterogeneity, nine haloalkaliphilic actinobacterial strains were characterized and identified. The PCR amplification of 16S rDNA using universal and genus-specific primers corroborated the trends that emerged through cultural and physiological characterization. Streptomyces was the most abundant genus amplified with the Streptomyces-specific primers (StreptB/E, StreptB/F), whereas other actinobacterial strains were amplified by the universal primers U1 and U2. Further molecular diversity was investigated by the gradient PCR-DGGE as a fingerprinting tool that generated group-specific DGGE patterns. Based on the nucleotide homology and phylogenetic analysis, strains OK-1 and OK-2 were identified as Streptomyces somaliensis. At the same time, OK-3 and OK-7 were detected as Streptomyces sp., while OK-5, OK-6, OK-8, OK-9, and OK-10 belonged to Nocardiopsis alba. Further, the cluster analysis using the UPGMA method generated 3 clusters based on biochemical characterization, sugar utilization, and enzyme production. The dendrogram based on the DGGE band pattern created with Jaccard-distance revealed two major clades with 33.33% similarity. Further, the study of alpha diversity calculation using phenotypic characteristics discloses highly diverse sugar utilization abilities. Moreover, a stress value of 0.1236 was obtained based on the NMDS analysis of the plots using Bary-Curties dissimilarity. Overall, the distinct phenotypic, metabolic, and molecular profiling illustrated the diversity among marine actinobacteria.
{"title":"Genetic and Physiological Diversity of Marine Actinobacteria from the Okha Coast, Gujarat, India","authors":"S. Gohel, V. Majithiya, Satya P. Singh","doi":"10.1080/01490451.2023.2218383","DOIUrl":"https://doi.org/10.1080/01490451.2023.2218383","url":null,"abstract":"Abstract Saline and alkaline habitats of the Okha coastline, Gujarat, India, were explored for the diversity of actinobacteria. Based on the phenotypic and genotypic heterogeneity, nine haloalkaliphilic actinobacterial strains were characterized and identified. The PCR amplification of 16S rDNA using universal and genus-specific primers corroborated the trends that emerged through cultural and physiological characterization. Streptomyces was the most abundant genus amplified with the Streptomyces-specific primers (StreptB/E, StreptB/F), whereas other actinobacterial strains were amplified by the universal primers U1 and U2. Further molecular diversity was investigated by the gradient PCR-DGGE as a fingerprinting tool that generated group-specific DGGE patterns. Based on the nucleotide homology and phylogenetic analysis, strains OK-1 and OK-2 were identified as Streptomyces somaliensis. At the same time, OK-3 and OK-7 were detected as Streptomyces sp., while OK-5, OK-6, OK-8, OK-9, and OK-10 belonged to Nocardiopsis alba. Further, the cluster analysis using the UPGMA method generated 3 clusters based on biochemical characterization, sugar utilization, and enzyme production. The dendrogram based on the DGGE band pattern created with Jaccard-distance revealed two major clades with 33.33% similarity. Further, the study of alpha diversity calculation using phenotypic characteristics discloses highly diverse sugar utilization abilities. Moreover, a stress value of 0.1236 was obtained based on the NMDS analysis of the plots using Bary-Curties dissimilarity. Overall, the distinct phenotypic, metabolic, and molecular profiling illustrated the diversity among marine actinobacteria.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"35 3","pages":"590 - 604"},"PeriodicalIF":2.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41278440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-23DOI: 10.1080/01490451.2023.2211076
A. Benaissa, Aicha Ouankilla, Zaineb Habibi, El-Hadja Benbessis, Abdallah Chegga
Abstract The soil represents a very important microbial niche, housing bacteria that can be a real source of biomolecules of interest. Indeed, this article represents a first bio-prospective study of a volcanic soil from the Tahart region (Tamanrasset, Algeria) which aims to research and characterize bacteria of the genus Bacillus and Pseudomonas producing enzymes and inhibitory substances. Diverse selective culture mediums were actually used to isolate Pseudomonas and Bacillus strains. These latters were phenotypically and an enzymatically characterized. The antibacterial characteristics of the pure culture, the supernatant, and the organic extracts were all investigated. The results showed that 60% of the bacteria from the genus Bacillus and Pseudomonas showed antimicrobial activity in vitro. On the other hand, our experimental results have revealed that the supernatant activity is slightly higher than that of pure culture and ethyl acetate showed a better extraction efficiency than chloroform and inhibitory zones, which vary between 7 and 25 mm in diameter. Moreover, the Bacillus antagonists represent 45% (B. thuringenis, B. sphericus, B. cereus, B. thiaminolyticus, B. anthracis and B. subtilis), followed by 32% of Pseudomonas bacteria (P. aeroginosa, P. luteola, P. flurescens). In conclusion, despite the acidity of the soil substrate, an interesting strains of bacteria producing inhibitory substances with enzymatic potential were selected.
{"title":"Screening for Antimicrobial and Enzymatic Activities of Bacillus and Pseudomonas Strains Isolated from Volcanic Soil of Tahart (Ahaggar, Algeria)","authors":"A. Benaissa, Aicha Ouankilla, Zaineb Habibi, El-Hadja Benbessis, Abdallah Chegga","doi":"10.1080/01490451.2023.2211076","DOIUrl":"https://doi.org/10.1080/01490451.2023.2211076","url":null,"abstract":"Abstract The soil represents a very important microbial niche, housing bacteria that can be a real source of biomolecules of interest. Indeed, this article represents a first bio-prospective study of a volcanic soil from the Tahart region (Tamanrasset, Algeria) which aims to research and characterize bacteria of the genus Bacillus and Pseudomonas producing enzymes and inhibitory substances. Diverse selective culture mediums were actually used to isolate Pseudomonas and Bacillus strains. These latters were phenotypically and an enzymatically characterized. The antibacterial characteristics of the pure culture, the supernatant, and the organic extracts were all investigated. The results showed that 60% of the bacteria from the genus Bacillus and Pseudomonas showed antimicrobial activity in vitro. On the other hand, our experimental results have revealed that the supernatant activity is slightly higher than that of pure culture and ethyl acetate showed a better extraction efficiency than chloroform and inhibitory zones, which vary between 7 and 25 mm in diameter. Moreover, the Bacillus antagonists represent 45% (B. thuringenis, B. sphericus, B. cereus, B. thiaminolyticus, B. anthracis and B. subtilis), followed by 32% of Pseudomonas bacteria (P. aeroginosa, P. luteola, P. flurescens). In conclusion, despite the acidity of the soil substrate, an interesting strains of bacteria producing inhibitory substances with enzymatic potential were selected.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"560 - 568"},"PeriodicalIF":2.3,"publicationDate":"2023-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43781209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1080/01490451.2023.2209750
Sunanda Mishra, Debasis Dash, A. Das
{"title":"Aquatic Microbial Diversity on Plastisphere: Colonization and Potential Role in Microplastic Biodegradation","authors":"Sunanda Mishra, Debasis Dash, A. Das","doi":"10.1080/01490451.2023.2209750","DOIUrl":"https://doi.org/10.1080/01490451.2023.2209750","url":null,"abstract":"","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47181754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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