Pub Date : 2023-07-15DOI: 10.1080/01490451.2023.2231925
Sujata Hota, K. Achary, Shikha Singh
{"title":"Identification and Molecular Characterization of Rhizopus delemar from Eastern Ghats of State of India and its Biotechnological Applications","authors":"Sujata Hota, K. Achary, Shikha Singh","doi":"10.1080/01490451.2023.2231925","DOIUrl":"https://doi.org/10.1080/01490451.2023.2231925","url":null,"abstract":"","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46053713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-15DOI: 10.1080/01490451.2023.2234371
Mandakini Gogoi, Dipankar Debbarma, S. Ray Chaudhuri
{"title":"Characterization of Target Organisms from Environmental Origin for Rapid Bioremediation of Hexamine","authors":"Mandakini Gogoi, Dipankar Debbarma, S. Ray Chaudhuri","doi":"10.1080/01490451.2023.2234371","DOIUrl":"https://doi.org/10.1080/01490451.2023.2234371","url":null,"abstract":"","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42275869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1080/01490451.2023.2240785
Afef Sai, Ali Ellafi, Y. Moussaoui, M. A. Borgi, Sonia Ben Younes
Abstract CPG’s wastewater from phosphate ore processing was characterized and its phytotoxicity before and after physicochemical treatment inside washing units was investigated for the first time. Mineralogical, spectral, physicochemical and microbiological characterizations showed that these wastewaters are relatively alkaline due to the high calcium and phosphorus contents. In fact, numerous minerals mainly fluorapatite, clinoptilolite and gypse were identified. In addition to its high salinity (EC = 9890 µS cm−1), the BOD5/COD ratio is equal to 0.949, which allows for the estimation that this effluent is potentially biodegradable. The microbiological characterization revealed the presence of revivable aerobic bacteria as; total coliforms (TC), fecal coliforms (FC), Pseudomonas, anaerobic sulfite bacteria (ASR), and fungi (F), whereas a complete absence of Salmonella, Vibrio cholera (CV), and Helminth egg (HE) was noted. In the second part, the influence of varying concentrations of phosphate wastewater was studied on radish, lentil, wheat and fenugreek seeds. Phosphate effluents were provided at 10%, 25%, 50%, 75% and 100%. Results were compared with a control group of the same seeds, irrigated with tap water. Based on several germination tests, plant irrigated with treated or untreated effluent exhibited a prominent reduction in growth traits at all four harvests. Except plant treated with treated effluent at 25% which generally induced no significant phytotoxicity to all species and which can play a fertilizer role. The adverse effects occurred with effluent-dose dependent manner. The high toxicity of untreated effluent compared to the treated effluent was inferred from the results. Nevertheless, the treated ffluent is still toxic, except at very low concentrations acting as a fertilizer. Graphical Abstract
摘要对CPG磷矿废水进行了表征，并首次对洗涤装置内理化处理前后的植物毒性进行了研究。矿物学、光谱、理化和微生物学表征表明，由于废水中钙、磷含量高，废水呈偏碱性。事实上，发现了许多矿物，主要是氟磷灰石、斜发沸石和石膏。除了其高盐度(EC = 9890µS cm - 1)外，BOD5/COD比值等于0.949，这使得该出水具有潜在的可生物降解性。微生物学特征显示存在可复性好氧细菌as;总大肠菌群(TC)、粪便大肠菌群(FC)、假单胞菌、厌氧亚硫酸盐细菌(ASR)和真菌(F)，而沙门氏菌、霍乱弧菌(CV)和蠕虫卵(HE)则完全不存在。第二部分研究了不同浓度的磷酸盐废水对萝卜、扁豆、小麦和葫芦巴种子的影响。提供10%，25%，50%，75%和100%的磷酸盐废水。结果与用自来水灌溉的相同种子的对照组进行了比较。根据几项发芽试验，用处理过的或未经处理的污水灌溉的植物在所有四次收获中都表现出生长性状的显著降低。除了用25%处理过的废水处理的植物，一般对所有物种都没有明显的植物毒性，可以起到肥料的作用。不良反应的发生与出水剂量有关。与处理过的废水相比，未经处理的废水毒性高，这是从结果中推断出来的。尽管如此，处理后的废水仍然是有毒的，除非浓度很低，起到肥料的作用。图形抽象
{"title":"Physico-Chemical Characterization and Phytotoxicity Assessment of Wastewater from Rock Phosphate Processing in Tunisia: From Seed Germination to Seedling Growth","authors":"Afef Sai, Ali Ellafi, Y. Moussaoui, M. A. Borgi, Sonia Ben Younes","doi":"10.1080/01490451.2023.2240785","DOIUrl":"https://doi.org/10.1080/01490451.2023.2240785","url":null,"abstract":"Abstract CPG’s wastewater from phosphate ore processing was characterized and its phytotoxicity before and after physicochemical treatment inside washing units was investigated for the first time. Mineralogical, spectral, physicochemical and microbiological characterizations showed that these wastewaters are relatively alkaline due to the high calcium and phosphorus contents. In fact, numerous minerals mainly fluorapatite, clinoptilolite and gypse were identified. In addition to its high salinity (EC = 9890 µS cm−1), the BOD5/COD ratio is equal to 0.949, which allows for the estimation that this effluent is potentially biodegradable. The microbiological characterization revealed the presence of revivable aerobic bacteria as; total coliforms (TC), fecal coliforms (FC), Pseudomonas, anaerobic sulfite bacteria (ASR), and fungi (F), whereas a complete absence of Salmonella, Vibrio cholera (CV), and Helminth egg (HE) was noted. In the second part, the influence of varying concentrations of phosphate wastewater was studied on radish, lentil, wheat and fenugreek seeds. Phosphate effluents were provided at 10%, 25%, 50%, 75% and 100%. Results were compared with a control group of the same seeds, irrigated with tap water. Based on several germination tests, plant irrigated with treated or untreated effluent exhibited a prominent reduction in growth traits at all four harvests. Except plant treated with treated effluent at 25% which generally induced no significant phytotoxicity to all species and which can play a fertilizer role. The adverse effects occurred with effluent-dose dependent manner. The high toxicity of untreated effluent compared to the treated effluent was inferred from the results. Nevertheless, the treated ffluent is still toxic, except at very low concentrations acting as a fertilizer. Graphical Abstract","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"706 - 718"},"PeriodicalIF":2.3,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44355782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1080/01490451.2023.2235345
Jia Liu, Xi'an Li, Xiuhua Liu, Wen Dong, Gang Li
Abstract Eolian sand is widely distributed in desert areas, and can easily trigger sandstorms under the combined effects of wind and thermal conditions. In this paper, an eolian sand solidification test via microbially induced calcium carbonate (CaCO3) precipitation (MICP) was conducted to explore prospective applications of MICP in the field of dust prevention and sand fixation. A bacterial growth test and a MICP mineralization reaction test were performed based on the response surface method and an orthogonal experiment. Based on eolian sand solidification test, the effects of cementation number and dry density on the permeability and strength characteristics of solidified eolian sand, as well as the content of CaCO3 generated were analyzed. The results showed that the bacteria grew best at a temperature of 35 °C, a pH of 9, and a shaking frequency of 170 rpm. Both urease activity and bacterial concentration increased as the temperature and pH increased but showed a trend of rising before falling as the shaking grew faster. The optimal conditions for MICP mineralization were a bacterial concentration of OD600=1.5, a consolidating fluid concentration of 1 mol/L, a reaction time of 16 h, a pH of 9, and a temperature of 25 °C. The permeability coefficient of solidified eolian sand decreased as the dry density and the number of cementations increased, whereas the CaCO3 content increased with the number of cementations. As the number of cementations increased, the unconfined compressive strength (UCS) of eolian sand initially rose before stabilizing and then increasing again. The CaCO3 content had a positive relationship with UCS, which with the correlation coefficient reached 0.80.
{"title":"Mechanical Properties of Eolian Sand Solidified by Microbially Induced Calcium Carbonate Precipitation (MICP)","authors":"Jia Liu, Xi'an Li, Xiuhua Liu, Wen Dong, Gang Li","doi":"10.1080/01490451.2023.2235345","DOIUrl":"https://doi.org/10.1080/01490451.2023.2235345","url":null,"abstract":"Abstract Eolian sand is widely distributed in desert areas, and can easily trigger sandstorms under the combined effects of wind and thermal conditions. In this paper, an eolian sand solidification test via microbially induced calcium carbonate (CaCO3) precipitation (MICP) was conducted to explore prospective applications of MICP in the field of dust prevention and sand fixation. A bacterial growth test and a MICP mineralization reaction test were performed based on the response surface method and an orthogonal experiment. Based on eolian sand solidification test, the effects of cementation number and dry density on the permeability and strength characteristics of solidified eolian sand, as well as the content of CaCO3 generated were analyzed. The results showed that the bacteria grew best at a temperature of 35 °C, a pH of 9, and a shaking frequency of 170 rpm. Both urease activity and bacterial concentration increased as the temperature and pH increased but showed a trend of rising before falling as the shaking grew faster. The optimal conditions for MICP mineralization were a bacterial concentration of OD600=1.5, a consolidating fluid concentration of 1 mol/L, a reaction time of 16 h, a pH of 9, and a temperature of 25 °C. The permeability coefficient of solidified eolian sand decreased as the dry density and the number of cementations increased, whereas the CaCO3 content increased with the number of cementations. As the number of cementations increased, the unconfined compressive strength (UCS) of eolian sand initially rose before stabilizing and then increasing again. The CaCO3 content had a positive relationship with UCS, which with the correlation coefficient reached 0.80.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"688 - 698"},"PeriodicalIF":2.3,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48762582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1080/01490451.2023.2236683
Nayereh Sadeghian, M. Mohammadi-Sichani
Abstract Bioremediation is one of the most effective approaches to eliminate or reduce soil contamination. Samples were collected from petroleum-contaminated soil in Oil Refinery Plant in Isfahan, Iran. The soil samples were cultured and the isolates were identified by polymerase chain reaction method. Total petroleum hydrocarbons assessment and measurement of the absorbance at 420 nm were used to determine the capability of fungi for bioremediation of petroleum hydrocarbons. Five isolates of fungi were purified from petroleum-contaminated soils. The oil degrading fungal isolates were identified as Aspergillus niger, Rhodotorula mucilaginosa, Penicillium sp., Penicillium chrysogenum, and Mucor circinelloides. All isolates had mycoremediation activity among which, Aspergillus niger, Mucor circinelloides, and Penicillium sp. had the highest potential to reduce total petroleum hydrocarbons (85%, 64%, and 53%, respectively) during 3 months. In addition, biosurfactant-producing ability was found only in these three isolates. The A. niger isolate had a high ability in hydrocarbons removal and, the P. chrysogenum isolate had significant ability in biosurfactant production, they can be applied in the bioremediation of contaminated soil in microbial consortia. The use of native microorganisms living in contaminated soils, especially fungi, have a high efficiency in removing contaminants of petroleum hydrocarbons in the soil.
{"title":"Mycoremediation of Iranian Crude Oil Contaminated Soil by Indigenous Fungi Isolates and the Evaluation of Their Bioremediation Ability","authors":"Nayereh Sadeghian, M. Mohammadi-Sichani","doi":"10.1080/01490451.2023.2236683","DOIUrl":"https://doi.org/10.1080/01490451.2023.2236683","url":null,"abstract":"Abstract Bioremediation is one of the most effective approaches to eliminate or reduce soil contamination. Samples were collected from petroleum-contaminated soil in Oil Refinery Plant in Isfahan, Iran. The soil samples were cultured and the isolates were identified by polymerase chain reaction method. Total petroleum hydrocarbons assessment and measurement of the absorbance at 420 nm were used to determine the capability of fungi for bioremediation of petroleum hydrocarbons. Five isolates of fungi were purified from petroleum-contaminated soils. The oil degrading fungal isolates were identified as Aspergillus niger, Rhodotorula mucilaginosa, Penicillium sp., Penicillium chrysogenum, and Mucor circinelloides. All isolates had mycoremediation activity among which, Aspergillus niger, Mucor circinelloides, and Penicillium sp. had the highest potential to reduce total petroleum hydrocarbons (85%, 64%, and 53%, respectively) during 3 months. In addition, biosurfactant-producing ability was found only in these three isolates. The A. niger isolate had a high ability in hydrocarbons removal and, the P. chrysogenum isolate had significant ability in biosurfactant production, they can be applied in the bioremediation of contaminated soil in microbial consortia. The use of native microorganisms living in contaminated soils, especially fungi, have a high efficiency in removing contaminants of petroleum hydrocarbons in the soil.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"699 - 705"},"PeriodicalIF":2.3,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46530494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Coal mining activities produce coal mine drainage which becomes a potential pollutant of regional groundwater. The temperature and microbial community in aquifers and rock formations change with coal mining depth increases. While the influence mechanisms of temperature and microorganisms on the hydrochemical characteristics of mine water are poorly understood. This study investigated a typical minefield in north China and performed the water–rock (coal) incubation experiments to explore the effect of temperature and microorganisms on the chemical characteristics of mine water. Hydrochemistry type of coal mine water in the goafs and main water-filled aquifers of Xinjulong coal mine was the SO4–Na type. Proteobacteria and Firmicutes were the main bacterial phyla in the coal mine water. The increasing closure time of goafs decreased SO4 2− concentration and increased the microbial diversity in mine water. The 28-day incubation experiments found Na+ and SO4 2− slightly increased by 5.13 and 6.67%, respectively, and HCO3 − decreased by 14.33% when the temperature rose by 10 °C. The Shannon index of the bacterial community in water–coal system increased by 6.7% and the relative abundance of Hydrogenophaga decreased by 22.7%, while that of Thiobacillus increased by 31.56%. The microbial activities decreased Na+ and SO4 2− by 2.31 and 4.95%, respectively, but increased HCO3 − by 7.59%. The functional prediction showed the microorganisms undergone sulfur conversion reactions and affected the dissolution of ions in mine water. The results were crucial for understanding the mutual effects of temperature and microorganisms on the hydrochemical characteristics of coal mine water. GRAPHICAL ABSTRACT
{"title":"Mutual Effects of Temperature and Microorganisms on the Chemical Characteristics of Coal Mine Drainage","authors":"Yanqing Ding, Yuqing Zhang, Mengqing Zhong, Puyu Qi, Zhimin Xu","doi":"10.1080/01490451.2023.2235338","DOIUrl":"https://doi.org/10.1080/01490451.2023.2235338","url":null,"abstract":"Abstract Coal mining activities produce coal mine drainage which becomes a potential pollutant of regional groundwater. The temperature and microbial community in aquifers and rock formations change with coal mining depth increases. While the influence mechanisms of temperature and microorganisms on the hydrochemical characteristics of mine water are poorly understood. This study investigated a typical minefield in north China and performed the water–rock (coal) incubation experiments to explore the effect of temperature and microorganisms on the chemical characteristics of mine water. Hydrochemistry type of coal mine water in the goafs and main water-filled aquifers of Xinjulong coal mine was the SO4–Na type. Proteobacteria and Firmicutes were the main bacterial phyla in the coal mine water. The increasing closure time of goafs decreased SO4 2− concentration and increased the microbial diversity in mine water. The 28-day incubation experiments found Na+ and SO4 2− slightly increased by 5.13 and 6.67%, respectively, and HCO3 − decreased by 14.33% when the temperature rose by 10 °C. The Shannon index of the bacterial community in water–coal system increased by 6.7% and the relative abundance of Hydrogenophaga decreased by 22.7%, while that of Thiobacillus increased by 31.56%. The microbial activities decreased Na+ and SO4 2− by 2.31 and 4.95%, respectively, but increased HCO3 − by 7.59%. The functional prediction showed the microorganisms undergone sulfur conversion reactions and affected the dissolution of ions in mine water. The results were crucial for understanding the mutual effects of temperature and microorganisms on the hydrochemical characteristics of coal mine water. GRAPHICAL ABSTRACT","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"676 - 687"},"PeriodicalIF":2.3,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44399053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1080/01490451.2023.2229835
Osama A. Al-Bedak, Samia El-Helaly, A. A. Saleh, E. Ahmed, M. A. Moneim, Mostafa R. Abukhadra
Abstract In this investigation, samples of mining dolomite wastes were collected from Abu Tartur phosphate mines, Egypt. The samples were thermally decomposed to MgO/CaO mixture as evidenced by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The MgO/CaO combination mixture was utilized in this study as an antibacterial agent against Bacillus cereus, Staphylococcus aureus, and Staphylococcus epidermidis. Antibacterial experiments have revealed that the MgO/CaO combination was more effective against Bacillus cereus, Staphylococcus aureus, and Staphylococcus epidermidis. For the three bacterial strains, the minimum inhibitory concentration (MIC) of the tested mixture was found to be 1.0 µg/mL. Acridine Orange pictures, which helped to explain the high occurrence of dead cells, and were utilized effectively to record the effect of MgO/CaO, which was employed in this investigation on the bacterial growth. Taking into consideration, MgO/CaO combination was found to be very interesting candidate material for bacterial disinfection due to its simplicity of synthesis, low cost, low release rate, and as excellent antibacterial activity.
{"title":"Utilization of Mining Rock Wastes as Antibacterial Agents Against Gram Positive Bacteria","authors":"Osama A. Al-Bedak, Samia El-Helaly, A. A. Saleh, E. Ahmed, M. A. Moneim, Mostafa R. Abukhadra","doi":"10.1080/01490451.2023.2229835","DOIUrl":"https://doi.org/10.1080/01490451.2023.2229835","url":null,"abstract":"Abstract In this investigation, samples of mining dolomite wastes were collected from Abu Tartur phosphate mines, Egypt. The samples were thermally decomposed to MgO/CaO mixture as evidenced by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The MgO/CaO combination mixture was utilized in this study as an antibacterial agent against Bacillus cereus, Staphylococcus aureus, and Staphylococcus epidermidis. Antibacterial experiments have revealed that the MgO/CaO combination was more effective against Bacillus cereus, Staphylococcus aureus, and Staphylococcus epidermidis. For the three bacterial strains, the minimum inhibitory concentration (MIC) of the tested mixture was found to be 1.0 µg/mL. Acridine Orange pictures, which helped to explain the high occurrence of dead cells, and were utilized effectively to record the effect of MgO/CaO, which was employed in this investigation on the bacterial growth. Taking into consideration, MgO/CaO combination was found to be very interesting candidate material for bacterial disinfection due to its simplicity of synthesis, low cost, low release rate, and as excellent antibacterial activity.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"667 - 675"},"PeriodicalIF":2.3,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47211024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Soil microorganisms contribute substantially to a wide range of services and thereby improve recovery in ecosystem restoration. However, there is relatively limited information on how microbial communities respond to different land-use types with similar plant species and their potential roles in supporting soil restoration in mine tailings. To understand the importance of the response of soil microbial communities to the stress of tailings area in different soil habitats, this article selected three different land use types (Tailings area, Buffer zone and Vegetable field) as the variables in the Yanzibian tailings area in Ningqiang, Shaanxi Province, China. Soil physicochemical properties and heavy metal concentrations of the different land use types were compared. Illumina MiSeq. 2500 Sequencing Technology was used to analyze the abundance and structural diversity of the microbial community in soil samples. The results showed that most of the soil samples were unsafe with multiple metals. All soil samples in the buffer zone and tailings ponds were acidic. With the acidity decreased, bacterial community richness and diversity increased significantly. Briefly, different environmental factors and soil microbial communities were significantly distinct across different land-use types. Cu, Zn, Pb, pH, MC (Moisture content), TN (Total nitrogen), TP (Total phosphorus), and TK (Total potassium) were essential factors affecting the abundance and structural diversity of soil microbial communities across three different land-use types. As a result, Actinobacteria, Alphaproteobacteria, and Gammaproteobacteria were the top dominant classes in the buffer zone and Vegetable field soils, while Sulfobacillia dominated in tailings pond soils. Further, we found that with the continuous decline of the pH, the dominant genera transferred from norank_f__norank_o__Vicinamibacterales, Bacillus and Nocardioides to the norank_f__norank_o__Gaiellales and norank_f__norank_o__Acidobacteriales, then to Sulfobacillus. These confirmed that the responses of microbiota to heavy metals stress varied in different land-use types. Together, this study provides important information on the occurrence and distribution of soil microbiomes in tailings areas and their potentially beneficial roles in soil restoration.
{"title":"Environmental Parameters Determine the Structure of Soil Bacteria under Different Land use Types in Tailings Area","authors":"Xiaoxiao Li, Jing Xu, Lu Li, Xinyue Zhang, Yuanyuan Shen, Haijuan Li, Yingna Wan, Tianpeng Gao","doi":"10.1080/01490451.2023.2227617","DOIUrl":"https://doi.org/10.1080/01490451.2023.2227617","url":null,"abstract":"Abstract Soil microorganisms contribute substantially to a wide range of services and thereby improve recovery in ecosystem restoration. However, there is relatively limited information on how microbial communities respond to different land-use types with similar plant species and their potential roles in supporting soil restoration in mine tailings. To understand the importance of the response of soil microbial communities to the stress of tailings area in different soil habitats, this article selected three different land use types (Tailings area, Buffer zone and Vegetable field) as the variables in the Yanzibian tailings area in Ningqiang, Shaanxi Province, China. Soil physicochemical properties and heavy metal concentrations of the different land use types were compared. Illumina MiSeq. 2500 Sequencing Technology was used to analyze the abundance and structural diversity of the microbial community in soil samples. The results showed that most of the soil samples were unsafe with multiple metals. All soil samples in the buffer zone and tailings ponds were acidic. With the acidity decreased, bacterial community richness and diversity increased significantly. Briefly, different environmental factors and soil microbial communities were significantly distinct across different land-use types. Cu, Zn, Pb, pH, MC (Moisture content), TN (Total nitrogen), TP (Total phosphorus), and TK (Total potassium) were essential factors affecting the abundance and structural diversity of soil microbial communities across three different land-use types. As a result, Actinobacteria, Alphaproteobacteria, and Gammaproteobacteria were the top dominant classes in the buffer zone and Vegetable field soils, while Sulfobacillia dominated in tailings pond soils. Further, we found that with the continuous decline of the pH, the dominant genera transferred from norank_f__norank_o__Vicinamibacterales, Bacillus and Nocardioides to the norank_f__norank_o__Gaiellales and norank_f__norank_o__Acidobacteriales, then to Sulfobacillus. These confirmed that the responses of microbiota to heavy metals stress varied in different land-use types. Together, this study provides important information on the occurrence and distribution of soil microbiomes in tailings areas and their potentially beneficial roles in soil restoration.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"640 - 653"},"PeriodicalIF":2.3,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44496429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1080/01490451.2023.2227625
Cătălina Haidău, P. Bulzu, I. Mirea, R. Bucur, O. Moldovan
Abstract Studies on fossil bone microbial communities are scarce; even fewer studies were performed in cave deposits. For our research, sediments and fossil bones were sampled, and the whole community 16S rRNA gene-based metabarcoding analyses were performed on samples from Muierilor and Ursilor caves, some of Romania’s most important archaeological and paleontological sites. Most of the identified taxa belong to Bacteria, with Proteobacteria, Acidobacteriota, Bacteroidota, and Actinobacteriota amongst the most abundant phyla in bone samples from both caves. The sediment samples presented similar composition, with Proteobacteria and Acidobacteriota being the most abundant phyla. The inferred bacteriomes indicated the presence of environment-specific bacteria, typical bone colonizers, and bacteria found in soils and decomposing human remains or archaeological profiles as well as phosphate-solubilizing and organotrophic bacteria. Diversity indices indicated a higher diversity in bone samples from Muierilor Cave than in Ursilor Cave samples and sediment samples from both caves. Environmental conditions, especially air relative humidity, were also considered in explaining the bacteriome diversity in different cave settings. These findings help to understand fossil bones’ deposition and degradation in various environmental conditions. Furthermore, this is the first attempt to relate microenvironments and bacteria to preserving fossil bones from caves.
{"title":"Potential Environmental Drivers of Fossil Bones Degradation—A Metabarcoding Approach in Two Carpathian Caves","authors":"Cătălina Haidău, P. Bulzu, I. Mirea, R. Bucur, O. Moldovan","doi":"10.1080/01490451.2023.2227625","DOIUrl":"https://doi.org/10.1080/01490451.2023.2227625","url":null,"abstract":"Abstract Studies on fossil bone microbial communities are scarce; even fewer studies were performed in cave deposits. For our research, sediments and fossil bones were sampled, and the whole community 16S rRNA gene-based metabarcoding analyses were performed on samples from Muierilor and Ursilor caves, some of Romania’s most important archaeological and paleontological sites. Most of the identified taxa belong to Bacteria, with Proteobacteria, Acidobacteriota, Bacteroidota, and Actinobacteriota amongst the most abundant phyla in bone samples from both caves. The sediment samples presented similar composition, with Proteobacteria and Acidobacteriota being the most abundant phyla. The inferred bacteriomes indicated the presence of environment-specific bacteria, typical bone colonizers, and bacteria found in soils and decomposing human remains or archaeological profiles as well as phosphate-solubilizing and organotrophic bacteria. Diversity indices indicated a higher diversity in bone samples from Muierilor Cave than in Ursilor Cave samples and sediment samples from both caves. Environmental conditions, especially air relative humidity, were also considered in explaining the bacteriome diversity in different cave settings. These findings help to understand fossil bones’ deposition and degradation in various environmental conditions. Furthermore, this is the first attempt to relate microenvironments and bacteria to preserving fossil bones from caves.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"654 - 666"},"PeriodicalIF":2.3,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49127928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.1080/01490451.2023.2223206
Wenqiang Wang, Yongqin Liu, Keshao Liu, Qi Yan, M. Ji, Feng Wang, Zhihao Zhang
Abstract Cryoconite holes are biological hotspots on the glacier surface. Cryoconite bacterial communities are strongly spatially heterogeneous at the regional scale which is closely associated with environmental variables and geographic distance. However, knowledge about the distribution patterns of cryoconite bacterial communities at a small scale and the underlying drivers is still limited. Here, we investigated bacterial communities in cryoconite holes of two adjacent glaciers of Tangula mountain range, DKMD (Dongkemadi) and LXZLB (Longxiazailongba) glacier, on the Tibetan Plateau using amplicon sequencing of the 16S rRNA gene. Cryoconite bacteria on the two glaciers have similar alpha diversity, but significantly different community compositions. Geospatial factors were the most important drivers (24.0% of the community variation) for predicting the cryoconite bacterial beta diversity in both glaciers. Latitude was the primary driver of the beta diversity of bacterial communities in the DKMD glacier, while the depth of the cryoconite holes played a dominant role in the LXZLB glacier. Collectively, these results demonstrated cryoconite bacterial communities exhibited spatial heterogeneity even at the small local scale and highlighted the importance of morphological properties of cryoconite holes instructing the distribution of cryoconite bacterial communities.
{"title":"Bacterial Communities in Cryoconite Holes of the Adjacent Glaciers on the Tibetan Plateau","authors":"Wenqiang Wang, Yongqin Liu, Keshao Liu, Qi Yan, M. Ji, Feng Wang, Zhihao Zhang","doi":"10.1080/01490451.2023.2223206","DOIUrl":"https://doi.org/10.1080/01490451.2023.2223206","url":null,"abstract":"Abstract Cryoconite holes are biological hotspots on the glacier surface. Cryoconite bacterial communities are strongly spatially heterogeneous at the regional scale which is closely associated with environmental variables and geographic distance. However, knowledge about the distribution patterns of cryoconite bacterial communities at a small scale and the underlying drivers is still limited. Here, we investigated bacterial communities in cryoconite holes of two adjacent glaciers of Tangula mountain range, DKMD (Dongkemadi) and LXZLB (Longxiazailongba) glacier, on the Tibetan Plateau using amplicon sequencing of the 16S rRNA gene. Cryoconite bacteria on the two glaciers have similar alpha diversity, but significantly different community compositions. Geospatial factors were the most important drivers (24.0% of the community variation) for predicting the cryoconite bacterial beta diversity in both glaciers. Latitude was the primary driver of the beta diversity of bacterial communities in the DKMD glacier, while the depth of the cryoconite holes played a dominant role in the LXZLB glacier. Collectively, these results demonstrated cryoconite bacterial communities exhibited spatial heterogeneity even at the small local scale and highlighted the importance of morphological properties of cryoconite holes instructing the distribution of cryoconite bacterial communities.","PeriodicalId":12647,"journal":{"name":"Geomicrobiology Journal","volume":"40 1","pages":"632 - 639"},"PeriodicalIF":2.3,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44077440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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