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Toward holistic medicine and holistic biology: life sciences after precision medicine and systems biology 走向整体医学和整体生物学:继精准医学和系统生物学之后的生命科学
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2019-01-01 DOI: 10.1080/21553769.2019.1682064
B. Yuan
In the field of life sciences, systems biology has encountered insurmountable obstacles caused by the complexity and adaptability of organisms. In the near future, precision medicine is expected to establish a personalized state-description system and a corresponding targeted-treatment system, but the comprehensive treatment of disease will remain a significant and difficult problem that needs to be resolved. As the holistic modeling method in complexity science matures and its concordance with traditional Chinese medicine (TCM) methods is increasingly revealed, the introduction of this method into the life sciences will become a necessity for scientific development. The incorporation of this method in the fields of biology and medicine will lead to the emergence of ‘holistic biology’ and ‘holistic medicine’. Holistic medicine is a personalized medicine system that incorporates TCM’s holistic concept and treatment approaches based on syndrome differentiation. Holistic medicine fundamentally solves the problem of complex life system integration and transforms adaptability from an obstacle for understanding organisms into an indispensable tool for more effective regulation and control of organisms. As a result, systems biology and precision medicine will be followed by an era of holistic medicine and holistic biology.
在生命科学领域,由于生物体的复杂性和适应性,系统生物学遇到了不可逾越的障碍。在不久的将来,精准医学有望建立个性化的状态描述体系和相应的靶向治疗体系,但疾病的综合治疗仍将是一个需要解决的重大难题。随着复杂性科学整体建模方法的成熟及其与中医方法的一致性日益显现,将整体建模方法引入生命科学将成为科学发展的必然。这种方法在生物学和医学领域的结合将导致“整体生物学”和“整体医学”的出现。整体医学是一种融合中医整体理念和以辨证为基础的治疗方法的个性化医疗体系。整体医学从根本上解决了复杂的生命系统整合问题,将适应性从理解生物体的障碍转变为更有效地调节和控制生物体不可或缺的工具。因此,继系统生物学和精准医学之后,将迎来整体医学和整体生物学的时代。
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引用次数: 4
Application of phiC31 integrase system in stem cells biology and technology: a review phiC31整合酶系统在干细胞生物学与技术中的应用综述
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-01-01 DOI: 10.1080/21553769.2018.1447516
Adham Fani Maleki, M. Sekhavati
ABSTRACT Two specific characteristics of stem cells, self-renewal and their differentiation capacity have made them important for vast scientific areas, particularly in the molecular and cell biology of stem cells, as well as regenerative medicine. Gene targeting plays a pivotal role in creating modified stem cells, which are considered the mainstay in regenerative medicine. The phiC31 integrase system, as a site-specific recombinase, could be a straightforward and efficient approach for this aim. According to the high potential of the application of phiC31 integrase system in stem cells, the present paper reviews previous studies, placing the focus on stem cell types and their application.
干细胞的两大特性:自我更新和分化能力,使其在广泛的科学领域,特别是干细胞的分子和细胞生物学以及再生医学中占有重要地位。基因靶向在制造修饰干细胞中起着关键作用,这被认为是再生医学的支柱。phiC31整合酶系统作为一种位点特异性重组酶,可能是实现这一目标的一种简单有效的方法。鉴于phiC31整合酶系统在干细胞中的应用潜力巨大,本文综述了前人的研究成果,重点对干细胞类型及其应用进行了综述。
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引用次数: 1
Alpha brain oscillations, approach attitude, and locus of control affect self-perception of social efficacy in cooperative joint-action α脑振荡、接近态度和控制点影响合作联合行动中社会效能感的自我知觉
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-01-01 DOI: 10.1080/21553769.2018.1465858
M. Balconi, M. E. Vanutelli
ABSTRACT The neurophysiological basis of social ranking perception underlying the execution of cooperative joint-actions was explored in the present study. Self-perception of social ranking, personality trait (Behavioral Activation System (BAS) and locus of control (LoC)) and alpha brain oscillations were considered. Subjects were required to match their cognitive performance in terms of accuracy (error rate) and response time. A positive feedback condition of a better joint-performance was provided and compared to absence of feedback. It was found that higher BAS participants and higher internal LoC responded in greater measure to post-feedback condition with better real performance probably based on their sensitivity to rewarding for high-BAS and the increased sense of self-efficacy. Moreover, higher-BAS showed an increased frontal left activity when they perceived increased cooperative efficacy. The present results confirmed the tendency to modulate both self-perceived social position and real performance based on the personal attitudes and the frontal.
摘要本研究探讨了合作联合行动执行背后的社会等级感知的神经生理学基础。考虑了社会等级的自我感知、人格特征(行为激活系统(BAS)和控制点(LoC))和阿尔法大脑振荡。受试者被要求在准确性(错误率)和反应时间方面与他们的认知表现相匹配。提供了更好的关节性能的正反馈条件,并与没有反馈进行了比较。研究发现,较高的BAS参与者和较高的内部LoC对反馈后条件的反应更大,实际表现更好,这可能是基于他们对高BAS奖励的敏感性和自我效能感的增强。此外,当他们感知到合作效能的提高时,BAS越高,左额活动越大。目前的结果证实了基于个人态度和正面来调节自我感知的社会地位和实际表现的趋势。
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引用次数: 1
Assessing the impact of plant genetic diversity in shaping the microbial community structure of Vitis vinifera phyllosphere in the Mediterranean 植物遗传多样性对地中海葡萄叶层微生物群落结构影响的评估
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-01-01 DOI: 10.1080/21553769.2018.1552628
P. Singh, A. Gobbi, S. Santoni, L. Hansen, P. This, J. Péros
ABSTRACT The aerial surface of the plant (phyllosphere) is the habitat of complex microbial communities and the structure of this microbiome may be dependent on plant genetic factors, local environment or interactions between them. In this study, we explored the microbial diversity present in the phyllosphere of a very diverse set of grapevine cultivars representing the three genetic pools of the species, grown on an experimental plot at Montpellier (French Mediterranean region). We assessed microbiome variation in the phyllosphere using amplicon sequencing of the 16S rRNA gene and of the internal transcribed spacer (ITS), according to the grapevine genetic pools or cultivars, and organs (i.e. leaves and grape berries). The observed microbiome was complex; out of 542 bacterial genera; Pseudomonas, Pantoea, Sphingomonas, and Acinetobacter were the most abundant and almost ubiquitously present across the samples, and out of 267 fungal genera; Aureobasidium, Alternaria, Mycosphaerella and Aspergillus were most represented. Our results illustrated that the microbial taxa were almost uniformly distributed among the genetic pools and only a few cultivar or genetic pool level differences were found, but a very clear differential taxa abundance was found between the leaf and berry samples. Some genus level associations were also observed with certain genetic pools.
植物的空气表面(层球)是复杂微生物群落的栖息地,这种微生物群落的结构可能取决于植物遗传因素、当地环境或它们之间的相互作用。在这项研究中,我们在蒙彼利埃(法国地中海地区)的一个试验田上,探索了一组非常多样化的葡萄品种的层层圈中存在的微生物多样性,这些葡萄品种代表了该物种的三个遗传库。我们利用16S rRNA基因和内部转录间隔(ITS)的扩增子测序,根据葡萄基因库或品种和器官(即叶片和葡萄果实)评估了层球微生物组的变异。观察到的微生物群很复杂;在542个细菌属中;在267个真菌属中,假单胞菌、泛菌、鞘氨单胞菌和不动杆菌数量最多,几乎无处不在;其中以金黄色葡萄球菌(auobasidium)、互交菌(Alternaria)、真菌菌(Mycosphaerella)和曲霉(Aspergillus)最为常见。结果表明,微生物类群在遗传库中分布基本均匀,仅存在少量品种或遗传库水平差异,但在叶片和浆果样品之间存在非常明显的类群丰度差异。在某些遗传池中也观察到一些属水平的关联。
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引用次数: 18
Challenges associated with ceftriaxone resistance in Salmonella 沙门氏菌对头孢曲松耐药性的挑战
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2018-01-01 DOI: 10.1080/21553769.2018.1491427
Ayman Ahmad Al kraiem, Guang Yang, Fahd Al kraiem, Tie Chen
ABSTRACT Salmonella is the leading cause of typhoidal and non-typhoidal infections in the world. The entry of Salmonella into the bloodstream causes an invasive disease state, resulting to high morbidity and mortality rates, especially in children. Owing to the misuse of antibiotics, certain Salmonella serovars are multi-drug resistant and do not respond to traditional antibiotics, such as ampicillin and trimethoprim-sulphamethoxazole, presenting a significant challenge for healthcare practitioners in treating and controlling the spread of this disease. Therefore, expensive third-generation cephalosporins, such as ceftriaxone, are currently used to treat Salmonella infection. However, a novel serovar of Salmonella that resists ceftriaxone was recently identified in Saudi Arabia, indicating wide spread Salmonella resistance. A comprehensive literature review on ceftriaxone resistance in Salmonella is therefore necessary to reflect upon current challenges. In this report, we provide a summary of Salmonella incidence, mechanisms of ceftriaxone resistance in Salmonella, and current treatment options.
摘要沙门氏菌是世界上引起伤寒和非伤寒感染的主要原因。沙门氏菌进入血液会导致侵袭性疾病状态,导致高发病率和死亡率,尤其是在儿童中。由于滥用抗生素,某些沙门氏菌血清型对多种药物具有耐药性,对氨苄青霉素和甲氧苄啶-磺胺甲恶唑等传统抗生素没有反应,这给医疗从业者治疗和控制这种疾病的传播带来了重大挑战。因此,昂贵的第三代头孢菌素,如头孢曲松,目前用于治疗沙门氏菌感染。然而,最近在沙特阿拉伯发现了一种抗头孢曲松的新型沙门氏菌血清型,表明沙门氏菌的耐药性广泛传播。因此,有必要对沙门氏菌对头孢曲松的耐药性进行全面的文献综述,以反思当前的挑战。在本报告中,我们总结了沙门氏菌的发病率、沙门氏菌对头孢曲松的耐药性机制以及目前的治疗方案。
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引用次数: 11
Nanotechnology for human food: Advances and perspective 纳米技术用于人类食品:进展与展望
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2017-01-01 DOI: 10.1080/21553769.2017.1365775
I. Chung, G. Rajakumar, T. Gomathi, S. Park, Seung-Hyun Kim, M. Thiruvengadam
ABSTRACT Nanotechnology is an exciting and rapidly emerging field in agriculture and food science. The usage of nanoscale materials in sensing and detection applications is growing quickly, providing alternative methods to conventional techniques for detecting chemical and biological contaminants in foods, beverages, and other products. Nanotechnology has the potential to innovate the agricultural, feed, and food sectors (further stated as agri/feed/food). Applications that are marketed already feature original product packaging with antimicrobial nanoparticles, and agrochemicals and nutrients that have been nano-encapsulated. Many nano-enabled products are presently under research and development, and may be introduced into the industry in the future. As with any other structured product, for market sanctions, applications need to prove the safe use of such new products without posing unwarranted safety risks to the consumer or the environment. In this review, we summarize the uses of nanotechnology related to food and nutraceuticals, while also identifying the outstanding challenges.
纳米技术是农业和食品科学中一个令人兴奋和迅速崛起的领域。纳米材料在传感和检测应用中的应用正在迅速增长,为检测食品、饮料和其他产品中的化学和生物污染物提供了替代传统技术的方法。纳米技术具有创新农业、饲料和食品部门(进一步表述为农业/饲料/食品)的潜力。已经上市的应用程序的特点是带有抗菌纳米颗粒的原始产品包装,以及纳米封装的农用化学品和营养物质。许多纳米产品目前正在研究和开发中,并可能在未来引入该行业。与任何其他结构化产品一样,为了获得市场制裁,应用程序需要证明此类新产品的安全使用,而不会对消费者或环境造成不必要的安全风险。在这篇综述中,我们总结了纳米技术在食品和营养保健品方面的应用,同时也指出了突出的挑战。
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引用次数: 23
An overview of recent developments in metabolomics and proteomics – phytotherapic research perspectives 代谢组学和蛋白质组学的最新进展综述——植物治疗学研究前景
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2017-01-01 DOI: 10.1080/21553769.2017.1279573
M. Mumtaz, A. Hamid, M. Akhtar, F. Anwar, U. Rashid, Mizher Hezam Al-Zuaidy
ABSTRACT In recent years, medicinal plants have gained much attention as potential source of bioactives for the development of novel herbal drugs for primary healthcare. However, phytotherapeutic mechanisms of action of phytomedicines need to be explored comprehensively. Out of the available strategies for the said purpose, metabolomics is one, which results in the production of inclusive metabolite profiles providing a clear understanding of diagnostic changes in the levels of metabolites, leading to therapeutic monitoring of drug targets through the elucidation of metabolomic pathways. On the other hand, proteomics is also a powerful strategy to deal with systematic protein expression analysis and analyze different biomarkers compared to metabolomics during drug treatment. Currently, 1H nuclear magnetic resonance spectroscopy, mass spectrometry (MS), Fourier transform infrared spectroscopy, gas chromatography equipped with mass spectrometry, liquid chromatography coupled with MS and ultra-performance liquid chromatography-MS coupled with multivariate statistical techniques, such as principle component analysis, partial least square and orthogonal-partial least square are among the widely applicable analytical tools for metabolite profiling, whereas two-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight -MS are the most accepted analytical methods for proteomic biomarker investigation. The present review summarizes the recent developments and perspectives of the biomarker investigation strategies so as to elaborate their imperative role for sustainable herbal drug developments.
近年来,药用植物作为一种潜在的生物活性物质来源,为开发用于初级卫生保健的新型中草药而备受关注。然而,植物药的作用机制还有待全面探索。在用于上述目的的可用策略中,代谢组学是其中之一,它导致产生包容性代谢物谱,提供对代谢物水平的诊断变化的清晰理解,从而通过阐明代谢组学途径对药物靶点进行治疗性监测。另一方面,与代谢组学相比,蛋白质组学也是处理系统蛋白质表达分析和分析药物治疗过程中不同生物标志物的有力策略。目前,1H核磁共振谱、质谱、傅里叶变换红外光谱、配备质谱的气相色谱、液相色谱耦合质谱、超高效液相色谱-质谱耦合多元统计技术,如主成分分析、偏最小二乘法、正交偏最小二乘法等,是广泛应用于代谢物谱分析的分析工具。而双向电泳和基质辅助激光解吸电离飞行时间-质谱是蛋白质组学生物标志物研究中最常用的分析方法。本文综述了生物标志物研究策略的最新进展和展望,阐述了生物标志物在草药可持续开发中的重要作用。
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引用次数: 13
Automated plant identification using artificial neural network and support vector machine 基于人工神经网络和支持向量机的植物自动识别
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2017-01-01 DOI: 10.1080/21553769.2017.1412361
S. Kho, S. Manickam, Sorayya Malek, Mogeeb A. A. Mosleh, S. K. Dhillon
ABSTRACT Ficus is one of the largest genera in plant kingdom reaching to about 1000 species worldwide. While taxonomic keys are available for identifying most species of Ficus, it is very difficult and time consuming for interpretation by a nonprofessional thus requires highly trained taxonomists. The purpose of the current study is to develop an efficient baseline automated system, using image processing with pattern recognition approach, to identify three species of Ficus, which have similar leaf morphology. Leaf images from three different Ficus species namely F. benjamina, F. pellucidopunctata and F. sumatrana were selected. A total of 54 leaf image samples were used in this study. Three main steps that are image pre-processing, feature extraction and recognition were carried out to develop the proposed system. Artificial neural network (ANN) and support vector machine (SVM) were the implemented recognition models. Evaluation results showed the ability of the proposed system to recognize leaf images with an accuracy of 83.3%. However, the ANN model performed slightly better using the AUC evaluation criteria. The system developed in the current study is able to classify the selected Ficus species with acceptable accuracy.
榕树(Ficus)是植物界最大的属之一,全世界约有1000种。虽然大多数榕属植物的分类钥匙都是可用的,但非专业人员的解释非常困难和耗时,因此需要训练有素的分类学家。本研究的目的是开发一个有效的基线自动化系统,利用图像处理和模式识别方法来识别三种具有相似叶片形态的榕属植物。选取了本雅明榕(F. benjamina)、透明孔榕(F. pellucidopunctata)和苏门答腊榕(F. sumatrana) 3种不同榕属植物的叶片图像。本研究共使用54个叶片图像样本。该系统主要分为图像预处理、特征提取和识别三个步骤。人工神经网络(ANN)和支持向量机(SVM)是实现的识别模型。评估结果表明,该系统识别树叶图像的准确率为83.3%。然而,使用AUC评价标准,ANN模型的表现略好。目前研究中开发的系统能够以可接受的精度对选定的榕树物种进行分类。
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引用次数: 49
Healthy lifestyle and eating perceptions: correlations with weight and low-fat and low-sugar food consumption in adolescence 健康的生活方式和饮食观念:与青少年体重、低脂和低糖食物消费的相关性
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2017-01-01 DOI: 10.1080/21553769.2017.1329170
I. Küster, N. Vila
ABSTRACT Background: Low-fat and low-sugar foods, marketed as health promoters, offer an interesting avenue for consumers to pursue a healthier life. Despite the relevance of educating adolescents about the importance of a healthier lifestyle, even today little attention is paid to this issue. The aim of this paper is to analyse adolescent consumers with varying degrees of a healthy lifestyle and different healthy eating perceptions and the relationship with their body mass index (BMI) and their low-fat and low-sugar food consumption. Methods: With a sample of 590 (353 from public schools and 237 from private schools) young consumers (13–17 years old) and SSS statistical tools, some interesting results are obtained. Results: Data obtained confirm the relationships among healthy life style activities and BMI and low food consumption. Also, data show interesting relationships among healthy eating perceptions and BMI and low food intake. Conclusions: The empirical results and findings from this study would be valuable for marketers and administration in the food industry to formulate marketing communication strategies and to promote a healthier lifestyle and eating perceptions among adolescents.
摘要背景:低脂低糖食品作为健康促进食品,为消费者追求更健康的生活提供了一条有趣的途径。尽管教育青少年健康生活方式的重要性很重要,但即使在今天,人们也很少关注这个问题。本文的目的是分析不同程度的健康生活方式和不同健康饮食观念的青少年消费者,以及他们与体重指数(BMI)和低脂、低糖食品消费的关系。方法:采用590名(353名来自公立学校,237名来自私立学校)年轻消费者(13-17岁)和SSS统计工具,获得了一些有趣的结果。结果:获得的数据证实了健康生活方式活动与BMI和低食物消耗之间的关系。此外,数据显示,健康饮食观念与BMI和低食物摄入之间存在着有趣的关系。结论:本研究的实证结果和发现将对食品行业的营销人员和管理人员制定营销沟通策略,促进青少年更健康的生活方式和饮食观念具有价值。
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引用次数: 21
How the pine seeds attach to/detach from the pine cone scale? 松树种子是如何附着在/从松果鳞片上分离的?
Q1 Biochemistry, Genetics and Molecular Biology Pub Date : 2017-01-01 Epub Date: 2017-02-13 DOI: 10.1080/21553769.2017.1287777
Kahye Song, Shyr-Shea Chang, Sang Joon Lee

One of the primary purposes of pine cones is the protection and distant dispersal of pine seeds. Pine cones open and release their embedded seeds on dry and windy days for long-distance dispersal. In this study, how the pine seed attach to/ detach from the pine cone scale for efficient seed dispersal were experimentally investigated by using X-ray micro-imaging technique. The cone and seeds adhere to one another in the presence of water, which could be explained by the surface tension and the contact angle hysteresis. Otherwise, without water, the waterproof seed wing surface permits rapid drying for detach and dispersion. On the other hand, during wildfires, pine cones open their seed racks and detach the pine seeds from pine cones for rapid seed dispersal. Due to these structural advantages, pine seeds are released safely and efficiently on adjust condition. These advantageous structure could be mimicked in practical applications.

松果的主要目的之一是保护和远距离传播松树种子。在干燥多风的日子里,松果会张开,释放出埋在里面的种子,远距离传播。本研究利用x射线显微成像技术,对松子在松果鳞片上的附着/分离过程进行了实验研究。在有水存在的情况下,球果和种子相互粘附,这可以用表面张力和接触角滞后来解释。否则,没有水,防水种子翼表面允许快速干燥分离和分散。另一方面,在野火期间,松果打开它们的种子架,将松子从松果上分离出来,以快速传播种子。由于这些结构上的优势,松子可以在调节条件下安全有效地释放。这些有利的结构可以在实际应用中模仿。
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引用次数: 5
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