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ENHANCED ANTICANCER POTENCY OF GEMCITABINE IN COMBINATION WITH PROPOFOL IN PROSTATE CANCER 吉西他滨联合异丙酚对前列腺癌的抗癌作用增强
Pub Date : 2021-04-09 DOI: 10.15671/HJBC.796377
E. Karataş, K. Bayindirli
I n the present study, the effects of propofol which is an intravenous anesthetic on its own and simultaneously with gemcitabine were investigated on human prostate cancer brain metastasis DU145 and bone metastasis PC3 cells at both cellular and molecular levels. In the first stage of our study, toxic doses of these agents were determined by using the CVDK-8 and lactate dehydrogenase release test. In the following phases, TAC and TOS analyzes were performed to determine the biochemical effects of these agents on cell lines, and also western blot analysis was used to show the inhibition of important oncogenic PI3K/AKT/mTOR pathway in cells treated with these agents. Propofol was found to increase the effectiveness of gemcitabine in both cells. When propofol and gemcitabine were administered simultaneously at high concentrations, they reduced cell viability and increased LDH activity. According to the results obtained from the western blot analysis, the combination of these two agents was found to lead to synergistic inhibition of the PI3K/Akt/mTOR pathway.
本研究从细胞和分子水平研究了静脉麻醉药异丙酚单用及与吉西他滨联用对人前列腺癌脑转移DU145和骨转移PC3细胞的影响。在我们研究的第一阶段,使用CVDK-8和乳酸脱氢酶释放试验来确定这些药物的毒性剂量。在接下来的阶段,我们通过TAC和TOS分析来确定这些药物对细胞系的生化影响,并通过western blot分析来显示这些药物对细胞中重要的致癌PI3K/AKT/mTOR通路的抑制作用。研究发现异丙酚可以增加吉西他滨在两种细胞中的有效性。当异丙酚和吉西他滨同时以高浓度施用时,它们降低了细胞活力并增加了LDH活性。western blot分析结果显示,这两种药物联合使用可协同抑制PI3K/Akt/mTOR通路。
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引用次数: 0
Effects of smoke and heat-shock on germination in eight perennial Reseda species (Resedaceae) 烟熏和热激对8种多年生杉木种子萌发的影响
Pub Date : 2021-03-10 DOI: 10.15671/HJBC.768365
Emre Çilden, Gökhan Ergan, C. Ülgen, Ş. Yildirimli, Ç. Tavşanoğlu
Reseda is an understudied genus regarding germination ecology. We studied the germination response of eight perennial Reseda species to fire related cues by performing a germination experiment including two aqueous smoke treatments (at 1:5 and 1:20 concentrations), one heat shock (100°C for 5 min.) treatment, and two corresponding controls. Smoke slightly decreased germination percentage in R. aucheri ssp. rotundifolia and R. minoica, and the reduction in R. luteola was statistically significant (P < 0.01). In contrary, smoke promoted germination in R. coodei, R. globulosa, and R. orientalis slightly, and markedly in R. anatolica (from 3 to 11%; P = 0.055). Heat shock treatment did not affect the germination in three of four species tested, but significantly increased germination percentage in R. luteola in comparison to the control (P = 0.046). Consequently, there was no consistent germination response to smoke or heat shock in Reseda species studied. This study is the most comprehensive study tested fire-related germination of several Reseda species and provides the first germination results on seven Reseda species. Further studies that include more species and treatments would show a more completed figure for fire-related germination patterns in the genus Reseda.
在发芽生态学方面,Reseda是一个研究不足的属。本文研究了8种多年生芦苇(Reseda)对火灾相关线索的萌发响应,包括两种水烟处理(1:5和1:20浓度)、一种热休克处理(100°C, 5 min)和两种相应的对照。烟熏稍微降低了红曲的发芽率。圆叶黄和小叶黄,木犀草含量降低有统计学意义(P < 0.01)。与此相反,烟熏对褐家鼠、球状家鼠和东方家鼠的萌发促进作用较弱,而对小褐家鼠的萌发促进作用较显著(3% ~ 11%;P = 0.055)。热休克处理对3个品种的发芽率无显著影响,但显著提高了木耳的发芽率(P = 0.046)。因此,在研究的芦笋种中,没有一致的对烟雾或热休克的萌发响应。本研究是对几种芦苇属植物的火灾相关萌发进行的最全面的研究,并提供了7种芦苇属植物的首次萌发结果。进一步的研究,包括更多的物种和处理,将显示一个更完整的数字,火相关的萌发模式,在Reseda属。
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引用次数: 0
Faunistic study on the family Meloidae (Coleoptera) of Ankara province (Turkey) 土耳其安卡拉省蠓科(鞘翅目)的区系研究
Pub Date : 2021-03-04 DOI: 10.15671/HJBC.788113
M. Demir, M. Kabalak
Fabricius, 1775 is the most species-rich genus with 10 species. Cerocoma (s.str.) bernhaueri Pardo Alcaide, 1977, Alosimus luteus (Waltl, 1838), Teratolytta monticola Bologna, 2006, Hycleus polymorphus (Pallas, 1771), H. sexmaculatus (A.G. Olivier, 1811), Mylabris ( Micrabris ) unicolor Falder -mann, 1837, Meloe ( Eurymeloe ) mediterraneus (G. Muller, 1925) and Stenoria (s.str.) apicalis (Latreille, 1804) species were recorded from Central Anatolian Region of Turkey for the first time. Detailed locality records of M . ( Micrabris ) unicolor and S. (s.str.) apicalis in Turkey were given with this study for the first time. The most western distributions of Alosimus luteus and Teratolytta monticola were given with this study.
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引用次数: 0
Charge recombination suppressed CdSeS/CdSe/ZnS QDSSC design 电荷复合抑制CdSeS/CdSe/ZnS QDSSC设计
Pub Date : 2021-02-07 DOI: 10.15671/HJBC.795908
Erdem Eli̇bol
he interest in quantum sensitized solar cells (QDSSC), which theoretically to up to energy con version is it has theoretically high efficiency value, obtained in studies with QDSSCs is far these This shows that there are many difficulties to be solved in QDSSC technology. One of the main challenges in QDSSC technology is irradiated load recombination occurring in QDSSC. For this reason, in this study, it is about using CdSeS QDs as an alternative to the most used CdS QDs in the literature in order to supp ress the load recombination between TiO 2 surface and electrolyte and QD surfaces. In the study, while CdS and CdSeS QDs were coated on the TiO 2 surface with SILAR method, the previously synthesized CdSe QD was coated with chemical deep deposition method. Surfaces were last treated with ZnS QDs. An optimization study was carried out to determine the ideal number of CdSeS coatings for QDSSCs. As a result, the Jsc and Voc values for TiO 2 /CdSeS 4 /CdSe/ZnS QDSSCs were 8.799 mA/ cm 2 and 0.795 V, respectively, while the PCE value increased to 4.452%.
量子敏化太阳能电池(quantum sensitized solar cells, QDSSC)在理论上可以达到能量转换,具有理论上较高的效率值,在量子敏化太阳能电池的研究中获得的兴趣远远不够,这表明量子敏化太阳能电池技术还有许多有待解决的困难。QDSSC技术面临的主要挑战之一是在QDSSC中发生辐照负荷重组。因此,在本研究中,使用CdSeS量子点作为文献中最常用的CdS量子点的替代品,以支持tio2表面与电解质和量子点表面之间的负载复合。在本研究中,CdS和CdSeS量子点采用SILAR法在tio2表面进行包覆,而之前合成的CdSe量子点采用化学深沉积法进行包覆。表面最后用ZnS量子点处理。通过优化研究,确定了QDSSCs的理想CdSeS涂层数量。结果表明,tio2 /CdSeS 4 /CdSe/ZnS QDSSCs的Jsc和Voc值分别为8.799 mA/ cm2和0.795 V, PCE值提高到4.452%。
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引用次数: 2
Effect of various inhibitors on enzymatic browning, antioxidant activity and total phenol content of fresh-cut rocket salad (Eruca sativa Mill.) 各种抑制剂对鲜切红叶沙拉酶促褐变、抗氧化活性和总酚含量的影响
Pub Date : 2021-02-07 DOI: 10.15671/HJBC.649050
Arzu Altunkaya Dinçay
antioxidant activity of rocket significantly, Rocket phenolics were protected from oxidation by the treatments of these inhibitors.
这些抑制剂对火箭酚类物质的抗氧化活性有明显的保护作用。
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引用次数: 3
Investigating Usage Potential of Datura stramonium L. for Phytoremediation of 2,4-Dichlorophenol 曼陀罗在植物修复2,4-二氯酚中的应用潜力研究
Pub Date : 2021-01-18 DOI: 10.15671/HJBC.689446
Zeynep Ceylan, M. Moharramzadeh, Ö. Atici
In this work, the phytoremediation potential of 2,4-Dichlorophenol (2,4-DCP) from soil and wetlands by Datura stramonium L. (jimsonweed) was investigated. The medium of seedlings growing in a hydroponic system was adjusted to different concentrations (0.0, 75, 100, 125, 150, 175, 200, 225, 250 and 275 ppm) of 2,4-DCP. Four days later, the remediation rate of 2,4-DCP in the growth medium, and root-stem length, root-stem dry weight, lipid peroxidation (LPO), protein and photosynthetic pigment content of seedlings were evaluated. D. stramonium seedlings provided remediation of 2,4-DCP between 52-78% at all concentrations. In addition, the 2,4-DCP treatments inhibited the root-stem lengths and dry weights of seedlings compared to their controls, particularly at high doses such as 200-275 ppm, but not at low doses. The applications generally increased protein and LPO content of roots and leaves slightly, but did not affect chlorophyll. The results show that D. stramonium has a high usage potential for phytoremediation of 2,4-DCP.
研究了曼陀罗(Datura stramonium L.)对土壤和湿地中2,4-二氯酚(2,4- dcp)的修复潜力。在水培系统中调节培养基中2,4- dcp的浓度(0.0、75、100、125、150、175、200、225、250和275 ppm)。4 d后,测定生长培养基中2,4- dcp的修复率,以及幼苗的根茎长、根茎干重、脂质过氧化(LPO)、蛋白质和光合色素含量。谷草幼苗对2,4- dcp的修复效果在52-78%之间。此外,与对照相比,2,4- dcp处理抑制了幼苗的根茎长度和干重,特别是在200-275 ppm的高剂量下,但在低剂量下没有。施氮量总体上增加了根系和叶片的蛋白质和LPO含量,但对叶绿素含量没有影响。结果表明,麦草霉在植物修复2,4- dcp方面具有很大的利用潜力。
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引用次数: 3
N-Glycosylation Profiles of the Green Microalgae Chlorella Zofingiensis 绿色微藻小球藻的n -糖基化谱
Pub Date : 2021-01-18 DOI: 10.15671/HJBC.717600
M. Yaman, Erdinç Aladağ, H. M. Kayili, Y. Kadioglu, B. Salih
Abstract Nowadays, the use of microalgae species as raw materials in biopharmaceutical production is on the agenda. The reason behind this idea is that microalgae are cell factories that are able to efficiently utilize carbon dioxide for the production of numerous biologically active compounds. However, there are several problems that remain to be solved in the production of recombinant protein from microalgaes. One of the critical requirements is to produce a bio-compatible N-glycosylation profile from the secreted recombinant proteins. However, the knowledge about the glycosylation machinery and N-glycan profiles of microalgae spices are quite limited. In the study, it was aimed to characterize N-glycan profiles of a green microalgae, Chlorella zofingiensis. To achieve this, photoautotrophically grown Chlorella zofingiensis extracts including (glyco-)proteins were enzymatically deglycosylated and labelled with 2-aminobenzoic acid tag. Released N-glycans were purified with a HILIC-based approach and analyzed by MALDI-TOF(/TOF)-MS. The results showed that C. zofingiensis included oligomannosidic type N-glycan patterns. In addition, N-glycosylation profiles of C. zofingiensis by MALDI-MS revealed that most of the oligomannosidic N-glycans were phosphorylated. Oz Gunumuzde, mikroalg turlerinin biyofarmasotik uretiminde hammadde olarak kullanimi konusu tartisilmaktadir. .Bu fikrin altinda yatan temel neden mikro alglerin, biyolojik olarak aktif cok sayida bilesenin uretimi icin karbon dioksiti etkin bir sekilde kullanabilen hucre fabrikalari olmasidir. Bununla birlikte, mikroalglerden rekombinant protein uretiminde kullanilmasi icin cozulmesi gereken birkac sorun mevcuttur. Kritik gereksinimlerden birisi salgilanan rekombinant proteinlerden biyo-uyumlu bir N-glikozilasyon profile uretmektir. Ancak mikroalg turlerinin glikozilasyon makineleri ve N-glikan profilleri hakkindaki bilgiler oldukca sinirlidir. Bu calismada, yesil bir mikroalg turu olan Chlorella zofingiensis ‘in N-glikan profilinin karakterize edilmesi amaclandi. Bu amacla (gliko-)proteinleri iceren fotoototrofik olarak yetistirilmis Chlorella zofingiensis ekstreleri enzimatik olarak deglikozile edildi ve 2-aminobenzoik asit etiketi ile etiketlendi. Serbest hale getirilen N-glikanlar HILIC bazli bir yaklasimla saflastirildi ve MALDI-TOF (/TOF)-MS ile analiz edildi. Sonuclar C. zofingiensis'in oligomannozidik turde N-glikan modellerini icerdigini gostermistir. Ek olarak, MALDI-MS tarafindan C. zofingiensis'in N-glikosilasyon profilleri, oligomannosidik N-glikanlarin neredeyse yarisinin fosforilenmis oldugunu ortaya koymustur.
目前,利用微藻作为生物制药的原料已经提上了议事日程。这个想法背后的原因是微藻是细胞工厂,能够有效地利用二氧化碳来生产许多生物活性化合物。然而,利用微藻生产重组蛋白仍有几个问题有待解决。其中一个关键要求是从分泌的重组蛋白中产生生物相容的n -糖基化谱。然而,关于微藻香料的糖基化机制和n -聚糖谱的知识相当有限。在这项研究中,它的目的是表征绿色微藻,小球藻zofingiensis的n -聚糖谱。为了实现这一目标,光自养生长的小球藻(Chlorella zoofingiensis)提取物包括(glyco-)蛋白被酶解去糖基化并标记为2-氨基苯甲酸标签。释放的n -聚糖采用基于hilic的方法纯化,MALDI-TOF(/TOF)-MS分析。结果表明,zofingiensis具有寡糖型n -聚糖模式。此外,通过MALDI-MS分析,zofingiensis的n -糖基化谱显示,大多数寡糖甘醇n -聚糖被磷酸化。奥兹·古努穆兹德,微分子分子生物学家,用微分子分子生物学家,用分子生物学家,用分子生物学家,用分子生物学家,用分子生物学家,用分子生物学家,用分子生物学家,用分子生物学家,用分子生物学家,用分子生物学家,用分子生物学家,用分子生物学家。白桦样蛋白、微果皮重组蛋白尿苷、kullanilmasi、cozulmesi等基因在白桦属植物中的应用。【关键词】基因重组蛋白;基因重组蛋白;基因重组蛋白;用微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微微。但是,在N-glikan谱图中,我们发现了一种微球藻(Chlorella zofingiensis)在氮化钾的作用。但小球藻(zoofingiensis)、小球藻(ehreleri)、小球藻(olarak)、小球藻(olarak)、小球藻(olarak)、小球藻(olarak)、小球藻(deglakozile)、小球藻(2-氨基苯甲酰基(2-氨基苯甲酰基)、小球藻(2-氨基苯甲酰基)、小球藻(2-氨基苯甲酰基)、小球藻(2-氨基苯甲酰基)、小球藻(2-氨基苯甲酰基)、小球藻。用MALDI-TOF (/TOF)-MS进行分析。低聚异丙基异丙基异丙基异丙基异丙基异丙基异丙基异丙基异丙基。李建军,李建军,李建军,等。n - glikosilason profilleri, n - glikoanlarin, n - omansidik, N-glikanlarin, N-glikanlarin, N-glikanlarin。
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引用次数: 0
Phenol adsorption on magnetic biochar derived from olive pomace: Equilibrium, kinetic and thermodynamics 橄榄渣磁性生物炭对苯酚的吸附:平衡、动力学和热力学
Pub Date : 2021-01-05 DOI: 10.15671/hjbc.687387
G. Dinç, S. Yildiz, E. Yel
I n this study, magnetic biochar obtained from pyrolysis of pretreated olive pomace by iron chloride was used as adsorbent to remove phenol and the adsorption capacity of phenol was revealed. Batch experiments were performed as a function of pH, contact time, adsorbent dosage, temperature, and phenol concentration. Moreover, adsorption kinetics and thermodynamics of phenol adsorption onto magnetic biochar were also evaluated in the study. The optimum conditions for maximum adsorption capacity were obtained at pH of 5.7, dosage of biochar 0.14 g and 60 minutes contact time. In this study, three adsorption isotherms, namely Langmuir, Freundlich and Temkin, were applied to fit the equilibrium data of adsorption of phenol onto magnetic biochar. Results showed that correlation coefficients (R 2 ) for three isotherm models decreased with the temperature increment from 20°C to 40°C and the most suitable isotherm model for adsorption was Freundlich. As for kinetics of the adsorption process, the best described model was found as pseudo-second order. In adsorption thermodynamics part, the negative ΔH° and ΔG° values demonstrated that adsorption was exothermic, feasible and was more spontaneous at lower temperatures.
本研究以预处理橄榄渣经氯化铁热解得到的磁性生物炭为吸附剂去除苯酚,并对苯酚的吸附能力进行了研究。对pH值、接触时间、吸附剂用量、温度和苯酚浓度进行了批量实验。此外,还研究了磁性生物炭对苯酚的吸附动力学和热力学。最佳吸附条件为:pH = 5.7,炭投加量0.14 g,接触时间60 min。本研究采用Langmuir、Freundlich和Temkin三条吸附等温线拟合苯酚在磁性生物炭上吸附的平衡数据。结果表明:在20 ~ 40℃范围内,3种等温线模型的相关系数r2随温度升高而减小,最适合吸附的等温线模型为Freundlich模型;对于吸附过程动力学,最好的描述模型为准二级。在吸附热力学部分,负的ΔH°和ΔG°值表明吸附是放热的,可行的,并且在较低温度下更自然。
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引用次数: 3
Chiral Separations by Capillary Electrophoresis and related Techniques with Different Chiral Selectors: A Review 不同手性选择剂的毛细管电泳分离及相关技术综述
Pub Date : 2020-12-26 DOI: 10.15671/hjbc.815414
Koray Şarkaya, Ilgım Göktürk, F. Yılmaz, Adil Denizli
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引用次数: 1
Bioactive Compound Activity Inducement of Thermophile Cyanobacterium aponinum Under Stress Conditions 胁迫条件下嗜热蓝藻aponinum生物活性化合物活性的诱导
Pub Date : 2020-12-24 DOI: 10.15671/hjbc.696762
N. Kiliç, G. Dönmez
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引用次数: 3
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Hacettepe Journal of Biology and Chemistry
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