The binding properties of insulin hormone to the potential antidiabetic coumarin derivative umbelliferone (7hydroxycoumarin, 7HC) was investigated by absorption, fluorescence quenching and molecular docking methods. The negative signs of thermodynamic parameters (H and S) indicated that hydrogen bonds and van der Waals forces were dominant in the binding mode. The effect of common metal ions was investigated on binding parameters. According to the Förster’s theory; binding distance, r was obtained as 4.17 nm. The spectral data further supported by molecular docking calculations which show hydrogen bonds between 7HC and insulin.
{"title":"Investigation on the Binding properties of a Coumarin Derivative to Insulin by Spectroscopic and Computational Approaches","authors":"Elmas Gökoğlu, Buse Aklar, Tuğba Taşkın Tok","doi":"10.15671/hjbc.1272350","DOIUrl":"https://doi.org/10.15671/hjbc.1272350","url":null,"abstract":"The binding properties of insulin hormone to the potential antidiabetic coumarin derivative umbelliferone (7hydroxycoumarin, 7HC) was investigated by absorption, fluorescence quenching and molecular docking methods. The negative signs of thermodynamic parameters (H and S) indicated that hydrogen bonds and van der Waals forces were dominant in the binding mode. The effect of common metal ions was investigated on binding parameters. According to the Förster’s theory; binding distance, r was obtained as 4.17 nm. The spectral data further supported by molecular docking calculations which show hydrogen bonds between 7HC and insulin.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"786 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77529719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to verify the standard test method TS EN 12630 and determine the sugar contents of commercially available fruit juices by high pressure liquid chromatography. A laboratory should verify the standard test method parameters in order to show its performance for the analysis, under consideration. For this purpose, Sucrose, Glucose and Fructose were analysed in orange juices obtained from the market. The principle of the method is based on the separation of sugars on a cation-exchange resin by isocratic elution with mobile phase, detection using a differential refractive index (RI) detector and external standard method. Accuracy and precision were performed via intraday and inter day studies to determine of accuracy and the precision (generally accepted as repeatability and reproducibility) for the standard test method. The recovery values of the sugars added into juice sample were 92%, 99% and 96% for sucrose, glucose and fructose, respectively.
本研究旨在验证标准测试方法TS EN 12630，并采用高压液相色谱法测定市售果汁中的糖含量。实验室应验证标准测试方法参数，以显示其对所考虑的分析的性能。为此，我们分析了从市场上获得的橙汁中的蔗糖、葡萄糖和果糖。该方法的原理是通过流动相等密度洗脱、差示折射率(RI)检测器和外标法在阳离子交换树脂上分离糖。准确度和精密度通过日间和日间研究来确定标准测试方法的准确度和精密度(通常被认为是可重复性和可再现性)。添加到果汁样品中的糖对蔗糖、葡萄糖和果糖的回收率分别为92%、99%和96%。
{"title":"Ticari Meyve Sularında HPLC ile Şeker Analizi için Standart Bir Metot Teyidi","authors":"Tuğba Doğan, Tamay Şeker","doi":"10.15671/hjbc.1270348","DOIUrl":"https://doi.org/10.15671/hjbc.1270348","url":null,"abstract":"This study aimed to verify the standard test method TS EN 12630 and determine the sugar contents of commercially available fruit juices by high pressure liquid chromatography. A laboratory should verify the standard test method parameters in order to show its performance for the analysis, under consideration. For this purpose, Sucrose, Glucose and Fructose were analysed in orange juices obtained from the market. The principle of the method is based on the separation of sugars on a cation-exchange resin by isocratic elution with mobile phase, detection using a differential refractive index (RI) detector and external standard method. Accuracy and precision were performed via intraday and inter day studies to determine of accuracy and the precision (generally accepted as repeatability and reproducibility) for the standard test method. The recovery values of the sugars added into juice sample were 92%, 99% and 96% for sucrose, glucose and fructose, respectively.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"351 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82595715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Özge ÖZŞEN BATUR, İsmail Kiran, K. H. C. Başer, Fatih Demi̇rci̇
Cedryl formate is a synthetic fragrance compound, which is an ester derivative of the sesquiterpenoid cedrol, found in different parts of the cedar tree/cedarwood with a characteristic fragrance. Cedryl formate is used in cosmetics and personal care products like fragrances, perfumes, as well as in the chemical and agriculture industries in various forms. In the present work, the microbial transformation of the formate was evaluated by 13 fungal cultures to produce new derivatives. Among the evaluated, the plant pathogenic fungus Aspergillus niger NRRL 326, biotransformed to the metabolite 8-cedren-3β-ol derivative with 6.2% yield. The structure of the chromatographically purified metabolite was elucidated by NMR and spectroscopic methods.
{"title":"Sedril Formatın Mikrobiyal Dönüşümü","authors":"Özge ÖZŞEN BATUR, İsmail Kiran, K. H. C. Başer, Fatih Demi̇rci̇","doi":"10.15671/hjbc.1244894","DOIUrl":"https://doi.org/10.15671/hjbc.1244894","url":null,"abstract":"Cedryl formate is a synthetic fragrance compound, which is an ester derivative of the sesquiterpenoid cedrol, found in different parts of the cedar tree/cedarwood with a characteristic fragrance. Cedryl formate is used in cosmetics and personal care products like fragrances, perfumes, as well as in the chemical and agriculture industries in various forms. In the present work, the microbial transformation of the formate was evaluated by 13 fungal cultures to produce new derivatives. Among the evaluated, the plant pathogenic fungus Aspergillus niger NRRL 326, biotransformed to the metabolite 8-cedren-3β-ol derivative with 6.2% yield. The structure of the chromatographically purified metabolite was elucidated by NMR and spectroscopic methods.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86545954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the antidiabetic potential of aqueous extract of Origanum minutiflorum was tested using streptozotocin induced diabetic rats. Changes in body weight, blood glucose level, biochemical parameters, antioxidant enzyme activities and histopathological examination of kidney were evaluated. The administration of aqueous extract and carvacrol to diabetic group rats caused significant decrease in blood glucose, biochemical parameters of serum and significantly increased the concentration of high density lipoprotein cholesterol. The administration of aqueous extract and carvacrol significantly increased the antioxidant enzyme activity and reduced the MDA level. The histopathological analysis of kidney showed that aqueous extract and carvacrol had possible ameliorative effect against kidney damage. Aqueous extract is also showed α-amylase and α-glucosidase inhibitor activity with high antioxidant activity. These scientific results confirm the use of O. minutiflorum as adjuvant antidiabetic therapy.
{"title":"Antidiabetic and antioxidant effect of Origanum minutiflorum O. Schwarz & P. H. Davis in streptozotocin-induced diabetic rats","authors":"I. Güllü, S. Albayrak, E. Kaymak, A. Yay","doi":"10.15671/hjbc.1239345","DOIUrl":"https://doi.org/10.15671/hjbc.1239345","url":null,"abstract":"In this study, the antidiabetic potential of aqueous extract of Origanum minutiflorum was tested using streptozotocin induced diabetic rats. Changes in body weight, blood glucose level, biochemical parameters, antioxidant enzyme activities and histopathological examination of kidney were evaluated. The administration of aqueous extract and carvacrol to diabetic group rats caused significant decrease in blood glucose, biochemical parameters of serum and significantly increased the concentration of high density lipoprotein cholesterol. The administration of aqueous extract and carvacrol significantly increased the antioxidant enzyme activity and reduced the MDA level. The histopathological analysis of kidney showed that aqueous extract and carvacrol had possible ameliorative effect against kidney damage. Aqueous extract is also showed α-amylase and α-glucosidase inhibitor activity with high antioxidant activity. These scientific results confirm the use of O. minutiflorum as adjuvant antidiabetic therapy.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73577269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, a label-free electrochemical biosensor system based on a disposable indium tin oxide polyethylene terephthalate (ITO-PET) electrode modified with the 11-(triethoxysilyl) undecanal (11-TESU) agent was developed for the detection of tumour necrosis factor-alpha (TNF-α) in serum. The developed biosensor was observed with electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) techniques, square wave voltammetry (SWV) and single frequency impedance (SFI) technique which is utilized for the specific interaction between anti-TNF-α and TNF-α antigen. In addition, scanning electron microscopy was used to look at how the morphology of each ITO-PET surface changed (SEM). All parameters such as 11-TESU concentration, anti-TNF-α concentration and anti-TNF-α incubation time, were optimized. The biosensor system was characterized by measuring its linear determination range, repeatability, reproducibility, reusability, storage stability, and surface coverage. The TNF-αelectrochemical biosensor showed high levels of repeatability and reproducibility as well as a large dynamic range of detection (from 0.03 pg mL-1 to 3 pg mL-1). The LOD and LOQ for the biosensor were extremely low at 1x10-4 pg mL-1 and 5x10-4 pg mL-1, respectively. It was applied to real samples to determine whether the proposed biosensor would be useful in clinical settings.
{"title":"11-(triethoxysilyl) undecanal agent-based biosensor system using disposable ITO-PET electrode for tumour necrosis factor-alpha detection","authors":"Burçak Demirbakan","doi":"10.15671/hjbc.1240299","DOIUrl":"https://doi.org/10.15671/hjbc.1240299","url":null,"abstract":"In this study, a label-free electrochemical biosensor system based on a disposable indium tin oxide polyethylene terephthalate (ITO-PET) electrode modified with the 11-(triethoxysilyl) undecanal (11-TESU) agent was developed for the detection of tumour necrosis factor-alpha (TNF-α) in serum. The developed biosensor was observed with electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) techniques, square wave voltammetry (SWV) and single frequency impedance (SFI) technique which is utilized for the specific interaction between anti-TNF-α and TNF-α antigen. In addition, scanning electron microscopy was used to look at how the morphology of each ITO-PET surface changed (SEM). All parameters such as 11-TESU concentration, anti-TNF-α concentration and anti-TNF-α incubation time, were optimized. The biosensor system was characterized by measuring its linear determination range, repeatability, reproducibility, reusability, storage stability, and surface coverage. The TNF-αelectrochemical biosensor showed high levels of repeatability and reproducibility as well as a large dynamic range of detection (from 0.03 pg mL-1 to 3 pg mL-1). The LOD and LOQ for the biosensor were extremely low at 1x10-4 pg mL-1 and 5x10-4 pg mL-1, respectively. It was applied to real samples to determine whether the proposed biosensor would be useful in clinical settings.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74362209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaç: Fenilketonüri (PKU), fenilalanin hidroksilaz enzim eksikliği ile karakterize nadir görülen bir kalıtsal metabolik hastalıktır. Bu enzimin eksikliği, kan fenilalanin seviyesini yükselterek, beyinde fenilalanin birikmesine ve geri dönüşümsüz nörolojik hasar oluşmasına neden olur. . Bu çalışmada fenilketonüri modelinde beyin tiyol/disülfit dengesindeki değişimin belirlenmesi amaçlanmıştır. Yöntem: PKU modeli oluşturulmuş sıçan yavrularında (n:7) ve kontrol grubunda beyin total tiyol ve serbest tiyol seviyesi modifiye elman yöntemi ile ölçüldü. Total tiyol ve native tiyol seviyelerine göre disülfit seviyesi hesaplandı. Bulgular: PKU grubun beyin total tiyol seviyesi kontrol grubuna göre istatistiksel olarak azdır (*p=0.0369). PKU grubun beyin serbest tiyol seviyesi kontrol grubuna göre istatistiksel olarak azdır (****p
{"title":"Thiol/Disulfide Balance in Induced Phenylketonuria Model","authors":"Cigdem Cicek","doi":"10.15671/hjbc.1254604","DOIUrl":"https://doi.org/10.15671/hjbc.1254604","url":null,"abstract":"Amaç: Fenilketonüri (PKU), fenilalanin hidroksilaz enzim eksikliği ile karakterize nadir görülen bir kalıtsal metabolik hastalıktır. Bu enzimin eksikliği, kan fenilalanin seviyesini yükselterek, beyinde fenilalanin birikmesine ve geri dönüşümsüz nörolojik hasar oluşmasına neden olur. . Bu çalışmada fenilketonüri modelinde beyin tiyol/disülfit dengesindeki değişimin belirlenmesi amaçlanmıştır. \u0000Yöntem: PKU modeli oluşturulmuş sıçan yavrularında (n:7) ve kontrol grubunda beyin total tiyol ve serbest tiyol seviyesi modifiye elman yöntemi ile ölçüldü. Total tiyol ve native tiyol seviyelerine göre disülfit seviyesi hesaplandı. \u0000Bulgular: PKU grubun beyin total tiyol seviyesi kontrol grubuna göre istatistiksel olarak azdır (*p=0.0369). PKU grubun beyin serbest tiyol seviyesi kontrol grubuna göre istatistiksel olarak azdır (****p","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78492296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acacia gum (AG) was grafted with polyacrylamides with different catonic ratios obtained by using different amounts of acrylamide (AM) and methacrylatoethyl trimethyl ammonium chloride (DMC (cationic monomer)). The thermal properties of these grafted products were investigated by differential scanning calorimetry (DSC) and thermogravimetric (TGA) analyses. The formation of grafted products was confirmed by FTIR spectroscopy and thermal analysis. The effects of different cationic ratios of polyacrylamide on flocculation were evaluated. Flocculation performance was evaluated in kaolin suspension.
{"title":"Thermal and Flocculation Properties of Acacia Gum Grafted with Cationic Polyacrylamide","authors":"S. Uzan","doi":"10.15671/hjbc.1197157","DOIUrl":"https://doi.org/10.15671/hjbc.1197157","url":null,"abstract":"Acacia gum (AG) was grafted with polyacrylamides with different catonic ratios obtained by using different amounts of acrylamide (AM) and methacrylatoethyl trimethyl ammonium chloride (DMC (cationic monomer)). The thermal properties of these grafted products were investigated by differential scanning calorimetry (DSC) and thermogravimetric (TGA) analyses. The formation of grafted products was confirmed by FTIR spectroscopy and thermal analysis. The effects of different cationic ratios of polyacrylamide on flocculation were evaluated. Flocculation performance was evaluated in kaolin suspension.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87312425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Increasing number of evidence demonstrated increased SARS-CoV-2 infection risk in cancer. Despite various studies shed light on SARS-CoV-2 mediated pathways upregulated in cancer, there is still ongoing efforts to reveal underlying mechanisms of elevated risk for COVID-19 disease in cancer. Given critical role of CEACAM1 in immune exhaustion and immune deregulation observed both in cancer and COVID-19, systematic characterization of CEACAM1 in different malignancies was performed with an ultimate aim to identify the involvement of CEACAM1 in enhanced COVID-19 susceptibility in cancer patients. Here we show that CEACAM1 expression was upregulated in a number of TCGA samples. In addition, CEACAM1 expression was positively correlated with SARS-CoV-2 infection genes in TCGA samples. Single-cell RNA sequencing analysis results of COVID-19 positive patients indicated upregulation of CEACAM1 expression. Furthermore, CEACAM1 expression was associated with HAVCR2, an immune checkpoint marker, and there was a correlation between CEACAM1 and HAVCR2 levels in different TCGA samples. Collectively, CEACAM1 might provide increased susceptibility of COVID-19 disease in cancer patients which might be explained with its interaction with HAVCR2.
{"title":"Integrative profiling of CEACAM1 in different malignancies with implications on the SARS-CoV-2 infection genes ACE2 and TMPRSS2","authors":"A. Acar","doi":"10.15671/hjbc.1232843","DOIUrl":"https://doi.org/10.15671/hjbc.1232843","url":null,"abstract":"Increasing number of evidence demonstrated increased SARS-CoV-2 infection risk in cancer. Despite various studies shed light on SARS-CoV-2 mediated pathways upregulated in cancer, there is still ongoing efforts to reveal underlying mechanisms of elevated risk for COVID-19 disease in cancer. Given critical role of CEACAM1 in immune exhaustion and immune deregulation observed both in cancer and COVID-19, systematic characterization of CEACAM1 in different malignancies was performed with an ultimate aim to identify the involvement of CEACAM1 in enhanced COVID-19 susceptibility in cancer patients. Here we show that CEACAM1 expression was upregulated in a number of TCGA samples. In addition, CEACAM1 expression was positively correlated with SARS-CoV-2 infection genes in TCGA samples. Single-cell RNA sequencing analysis results of COVID-19 positive patients indicated upregulation of CEACAM1 expression. Furthermore, CEACAM1 expression was associated with HAVCR2, an immune checkpoint marker, and there was a correlation between CEACAM1 and HAVCR2 levels in different TCGA samples. Collectively, CEACAM1 might provide increased susceptibility of COVID-19 disease in cancer patients which might be explained with its interaction with HAVCR2.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90469119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Şener, M. Badem, M. Çatalbaş, Ş. Kanbolat, U. Özgen, Nevin ULAŞ ÇOLAK
Nowadays, one of the therapeutic approaches for obesity is the use pancreatic lipase inhibitors which reduce the digestion and absorption of fats. Most research indicates that natural sources which have a lipase inhibitory effect, may be utilized to treat obesity. Verbascum pyramidatum is one of the potential natural sources for obesity, and it has been demonstrated to have anti-inflammatory, antidiabetic, and regulatory effects on lipid metabolism. With this study, V. pyramidatum's potential lipase inhibitor effect, it is aimed to reveal its value in the treatment of obesity. In vitro spectroscopic method was used to determine the lipase inhibitory effect of V. pyramidatum. The quantitative investigation of V. pyramidatum's phenolic metabolites with antiobesity activity was carried out utilizing the Reverse Phase-High Performance Liquid Chromatography method. In this reported study, it was proven that extract and all fractions had an impact that inhibited lipase, with the ethyl acetate extract showing the highest inhibitory effect. Additionally, it was revealed through HPLC analysis that the species included p-OH benzoic acid, coumaric acid, quercetin, sinapic acid, and syringaldehyde. It has been demonstrated that V. pyramidatum may be a promising candidate for obesity treatment, but further investigations are required to use it as a therapeutic agent.
{"title":"Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity","authors":"S. Şener, M. Badem, M. Çatalbaş, Ş. Kanbolat, U. Özgen, Nevin ULAŞ ÇOLAK","doi":"10.15671/hjbc.1196913","DOIUrl":"https://doi.org/10.15671/hjbc.1196913","url":null,"abstract":"Nowadays, one of the therapeutic approaches for obesity is the use pancreatic lipase inhibitors which reduce the digestion and absorption of fats. Most research indicates that natural sources which have a lipase inhibitory effect, may be utilized to treat obesity. Verbascum pyramidatum is one of the potential natural sources for obesity, and it has been demonstrated to have anti-inflammatory, antidiabetic, and regulatory effects on lipid metabolism. With this study, V. pyramidatum's potential lipase inhibitor effect, it is aimed to reveal its value in the treatment of obesity. \u0000In vitro spectroscopic method was used to determine the lipase inhibitory effect of V. pyramidatum. The quantitative investigation of V. pyramidatum's phenolic metabolites with antiobesity activity was carried out utilizing the Reverse Phase-High Performance Liquid Chromatography method. \u0000In this reported study, it was proven that extract and all fractions had an impact that inhibited lipase, with the ethyl acetate extract showing the highest inhibitory effect. Additionally, it was revealed through HPLC analysis that the species included p-OH benzoic acid, coumaric acid, quercetin, sinapic acid, and syringaldehyde. It has been demonstrated that V. pyramidatum may be a promising candidate for obesity treatment, but further investigations are required to use it as a therapeutic agent.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89479161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The salt obtained from the salt sources are not desirable purity level and contains some impurities. In the scope of this study, coagulation of magnesium hydroxide (Mg(OH)2) in pH control has been carried out by adding sodium hydroxide (NaOH) to magnesium chloride (MgCl2) and by this way recovery process has been achieved by Self Tuning PID Control. To provide the best coagulation pH 11 has been obtained as optimum pH value in consideration of the performed calculations.
{"title":"SELF-TUNING PID (STPID) CONTROL OF PH IN RECOVERING PROCESS OF MAGNESIUM HYDROXIDE (MG(OH)2) BY COAGULATION FROM SALT","authors":"Yasemin Selay Çakiroğlu","doi":"10.15671/hjbc.1164234","DOIUrl":"https://doi.org/10.15671/hjbc.1164234","url":null,"abstract":"The salt obtained from the salt sources are not desirable purity level and contains some impurities. In the scope of this study, coagulation of magnesium hydroxide (Mg(OH)2) in pH control has been carried out by adding sodium hydroxide (NaOH) to magnesium chloride (MgCl2) and by this way recovery process has been achieved by Self Tuning PID Control. To provide the best coagulation pH 11 has been obtained as optimum pH value in consideration of the performed calculations.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76949202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：