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Efficacy of botanicals extracts against Spodoptera litura (Lepidoptera: Noctuidae) under laboratory condition 植物提取物在实验室条件下对斜纹夜蛾(鳞翅目:夜蛾科)的防治效果
Pub Date : 2022-11-29 DOI: 10.25081/jsa.2022.v6.8103
Arfa Safder, Zohaib Afzal, Fizza Munawar, Ahad Masood Hashmi, Rafia Bakht Ali, Nagina Amanat
Numerous cultivated plants are attacked by Spodoptera litura, which also significantly reduces crop yields. In this study, a green strategy was used to assess the toxicological impact of three plant extracts (Eucalyptus globulus, Allium sativum, and Azadirachta indica) against S. litura larvae in their second and third instars. The study resulted that among the tested extracts, neem (A. indica) was found to be the most toxic and efficient extract followed by E. globulus and A. sativum. 29.92, 56.61, and 79.53% mortality of 2nd instar larvae were recorded at 3, 5, and 7 ppm, respectively, while 24.52, 27.66, and 72.42% of 3rd instars. Among the tested extracts, A. sativum was the least toxic extract than others. One of the main causes of large losses in field crops is insect pest damage. According to the study’s findings, A. indica extract has the potential to lessen S. litura damage to crops as a natural substitute for chemical pesticides without harming beneficial species.
许多栽培植物受到斜纹夜蛾的侵害,这也大大降低了作物产量。采用绿色策略研究了三种植物提取物(蓝桉、葱和印楝)对斜纹夜蛾2龄和3龄幼虫的毒理学影响。研究结果表明,印度楝树提取物的毒性和药效最高,其次是黄楝树提取物和黄楝树提取物。3、5和7 ppm处理下,2龄幼虫死亡率分别为29.92%、56.61%和79.53%,3龄幼虫死亡率分别为24.52%、27.66%和72.42%。在所有被试提取物中,苜蓿提取物的毒性最小。造成大田作物重大损失的主要原因之一是虫害危害。根据这项研究的发现,籼稻提取物作为化学农药的天然替代品,有可能减轻斜纹紫穗病对作物的危害,而不会伤害有益的物种。
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引用次数: 1
Genetic divergence analyses in tef (Eragrostis tef (Zucc.) Trotter) landraces based on morpho-agronomic traits tef (Eragrostis tef (Zucc.))遗传分化分析基于形态农艺性状的地方品种
Pub Date : 2022-10-27 DOI: 10.25081/jsa.2022.v6.7595
Siraye Ashagrie, Fisseha Worede, Wossen Tarekegne
Tef (Eragrostis tef (Zucc.) Trotter) is a gluten-free cereal crop mainly grown in Ethiopia. Knowledge of the nature and magnitude of variation that exists in breeding materials is of great importance for effective breeding. This study was designed to assess the genetic divergence of tef landrace collections using quantitative and qualitative traits and to investigate the important trait in discriminating the tef landraces. The experiment was carried out at Adet Agricultural Research Center during the rainy season in 2019. Sixty-two landraces and two improved varieties were laid out in an 8×8 simple lattice design. Data were collected on 12 quantitative and three qualitative traits. The analysis of variance detected significant (P≤ 0.05) variation for panicle length and biomass yield, and highly significant (P≤ 0.01) differences among the tef landraces for the rest 10 traits, indicating the existence of appreciable genetic diversity that can be exploited in future tef breeding programs. Cluster analysis using the average linkage method grouped the 64 tef genotypes into four clusters. The highest inter-cluster distance was recorded between clusters III and IV; as a result, the tef genotypes from these clusters could be used as parents for future crossing programs. Principal components analysis revealed that the first four principal component axes accounted for 75.12% of the total variation among landraces, indicating that the traits considered were appropriate to detect the variation. The finding also demonstrated that grain yield, harvest index, days to heading, culm length and lodging index are higher contributors to the diversity of the tef landraces. These traits were most important in the discrimination of the landraces. Overall, the present study confirmed the presence of substantial diversity among the tef landraces.
Tef(朱克)牛蹄(Trotter)是一种主要生长在埃塞俄比亚的无麸质谷类作物。了解育种材料中存在的变异的性质和大小对有效育种非常重要。本研究旨在从数量性状和质量性状两方面评价tef地方品种的遗传分化,探讨辨别tef地方品种的重要性状。该实验是在2019年雨季期间在阿特农业研究中心进行的。62个地方品种和2个改良品种以8×8简单的格子设计排列。收集了12个定量性状和3个定性性状的数据。方差分析显示,各地方品种间穗长和生物量差异显著(P≤0.05),其余10个性状差异极显著(P≤0.01),表明地方品种间存在可观的遗传多样性,可为今后的tef育种提供参考。采用平均连锁法进行聚类分析,将64个tef基因型分为4个聚类。聚类III和聚类IV之间的聚类间距最大;结果表明,这些聚类的tef基因型可作为后代杂交的亲本。主成分分析表明,前4个主成分轴占各地方品种间总变异的75.12%,表明所考虑的性状适合检测变异。籽粒产量、收获指数、抽穗天数、茎秆长度和倒伏指数对tef地方品种的多样性贡献较大。这些特征在对本土种族的歧视中尤为重要。总的来说,本研究证实了tef地方种族之间存在着实质性的多样性。
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引用次数: 0
Wilt disease symptoms in red leaf lettuce (Lactuca sativa L.) after inoculated with Trichoderma longibrachiatum 红叶莴苣(Lactuca sativa L.)接种长尾木霉后的枯萎病症状
Pub Date : 2022-04-26 DOI: 10.25081/jsa.2023.v7.8271
Muhammad Iqbal Nul Hakim Mohd Sazili, F. A. Zakry, Franklin Ragai Kundat
Trichoderma is a fungal genus that is widely reported to cause beneficial impacts to crop plants but rarely detriment. The present study was conducted to assess the effect of inoculation of Trichoderma longibrachiatum strain UPMT14 on red leaf lettuce (Lactuca sativa L.) under growth room conditions. A culture of UPMT14 was grown on potato dextrose broth to produce inoculum. This liquid inoculum was injected through the stem of red leaf lettuce seedlings 22 days old with five replications, and then plant growth progress was monitored for vegetative responses. Red leaf lettuce plants began to exhibit foliar symptoms on day 36, such as chlorosis, wilt, and drying out, before total collapsed on day 45 in comparison to untreated control red leaf lettuce plants. In conclusion, the wilting incidence in red leaf lettuce was observed after T. longibrachiatum inoculation. Further studies are needed in future to understand the pathogenesis of T. longibrachiatum.
木霉是一种真菌属,被广泛报道对作物植物产生有益影响,但很少有害。在生长室内条件下,研究了接种长尾木霉UPMT14菌株对红叶莴苣的影响。在马铃薯葡萄糖肉汤上培养UPMT14以产生接种物。该液体接种剂通过22 d的红叶莴苣幼苗茎部注射,共5次重复,然后监测植株的生长过程,观察其营养反应。与未经处理的对照红叶生菜植株相比,红叶生菜植株在第36天开始表现出叶片症状,如黄化、枯萎和干燥,在第45天完全枯萎。综上所述,接种长尾曲霉后,观察到红叶莴苣的萎蔫发生率。长臂棘虫的发病机制有待进一步研究。
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引用次数: 0
Genetic analysis of yield related traits in landrace × commercial watermelon hybrids 地方×商品西瓜杂交品种产量相关性状的遗传分析
Pub Date : 2022-03-18 DOI: 10.25081/jsa.2022.v6.7477
T. Nyurura, M. Maphosa
Watermelon [Citrullus lanatus var lanatus L.] is a major xerophytic crop originating in Africa especially grown in subtropical and tropical parts of the world. However, few adapted varieties are available to promote greater yield and make watermelon a viable commercial crop of choice. Path analysis and combining ability was carried out for yield related traits among three popular local landraces; Landrace1, Landrace 2 and Landrace 3 collected in Zimbabwe and three commercial watermelon varieties ‘Congo’, ‘Charleston Grey’ and ‘Crimson Sweet’ in plots arranged in an RCBD replicated 3 times. A complete diallel mating design was used to generate 36 F1 genotypes which together with the parents were evaluated for several quantitate traits. Data on correlations, path analysis, GCA and SCA between fruit yield and other traits were computed using Microsoft Excel and GenStat 17. Individual fruit weight (FIW) and the number of fruits per plant (NF) were found to be significantly linked with final fruit yield. Significant positive correlations were also observed on NF and number of female flowers (NFF), number of vines (NV), and FIW and these traits could be recommended as reliable selection indicators for creating high yielding watermelon genotypes. Path analysis showed that FIW and NF were the most important contributors to watermelon yield. GCA and SCA effects were significant and positive for yield which indicates that the traits are controlled by both additive and non-additive genes. ‘Crimson Sweet’ and ‘Landrace 1’ were the best pollen sources whereas ‘Landrace 1’ and ‘Landrace 2’ were the highest yielders when used as female parents. Overall, results from this study identified usable yield related proxies and need to incorporate local landraces germplasm in watermelon improvement.
西瓜[Citrullus lanatus var lanatus L.]是一种主要的旱生作物,原产于非洲,特别是在世界亚热带和热带地区种植。然而,很少有适合的品种可以提高产量,使西瓜成为可行的商业作物选择。对3个地方流行品种的产量相关性状进行通径分析和配合力分析;在津巴布韦采集的长白1号、长白2号和长白3号,以及三个商业西瓜品种“刚果”、“查尔斯顿灰”和“深红色甜”,在RCBD中重复3次。采用完全双列杂交设计,获得36个F1基因型,并与亲本对若干定量性状进行了评价。利用Microsoft Excel和GenStat 17计算果实产量与其他性状的相关性、通径分析、GCA和SCA数据。单果重(FIW)和单株果数(NF)与最终果实产量显著相关。籽粒肥力与雌花数(NFF)、藤数(NV)和果实生物量(FIW)均呈显著正相关,可作为西瓜高产基因型的可靠选择指标。通径分析表明,FIW和NF对西瓜产量的影响最大。GCA和SCA对产量的影响显著且呈正相关,说明性状受加性和非加性基因的共同控制。‘深红甜’和‘长白1’是最佳花粉源,而‘长白1’和‘长白2’作为母本产量最高。总的来说,本研究的结果确定了可用的产量相关指标,需要在西瓜改良中纳入地方种质资源。
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引用次数: 0
Correlation and path coefficient studies for yield and its components of upland rice (Oryza sativa L.) in North Western Ethiopia 埃塞俄比亚西北部旱稻产量及其构成要素的相关及通径系数研究
Pub Date : 2022-02-25 DOI: 10.25081/jsa.2022.v6.7253
Desta Abebe Belete, Atsedemariam Tewachew, Mulugeta Bitew, Tafere Mulualem
Rice is the major source of calories and third largest crop after maize and wheat by productivity. The aim of the present study was to determine the correlations between grain yield and its contributing traits and to measure the direct and indirect effects of those traits on grain yield in upland rice. Sixteen upland rice genotypes were tested using RCBD with three replications at Pawe district (on station and on farm) during 2016/2017 main cropping season. The estimates of genotypic and phenotypic correlation coefficients between nine characters were computed and also the direct and indirect effects. The results obtained indicated that estimates of genotypic correlation coefficients between nine characters were generally different in sign but higher in magnitude than the corresponding phenotypic correlation coefficients. Days to maturity and plant height were showed strong positive and significant phenotypic and genotypic correlations with days to 50% heading at on farm level. Whereas, thousand seed weight was exhibited strong positive and significant phenotypic and genotypic correlations with days to 50% heading followed by panicle length at on station level. The highest positive both phenotypic and genotypic direct effect on grain yield were exerted by days to heading followed by panicle length and number of filled grain per panicle. In contrast, high order of negative both phenotypic and genotypic indirect effects were extended by panicle length on grain yield via days to maturity (-0.167), thousand seed weight (-0.162) and plant height (-0.09). The direct effects of the remaining six characters were too low to be considered important and the rest of the estimates of indirect effects obtained in path analysis were negligible. Generally, it can be concluded that there is a favorable situation for obtaining high response to selection in improving yield and its components in upland rice.
水稻是热量的主要来源,也是产量仅次于玉米和小麦的第三大作物。本研究的目的是确定旱稻籽粒产量与其贡献性状之间的相关性,并测量这些性状对旱稻籽粒产量的直接和间接影响。2016/2017年主要种植季,在Pawe区(站内和农场内)使用RCBD对16个旱稻基因型进行了3个重复试验。计算了9个性状间基因型和表型相关系数的估计值以及直接和间接效应。结果表明,9个性状之间的基因型相关系数估计值在符号上普遍不同,但在数量级上高于相应的表型相关系数。在田间水平上,成熟日数和株高与抽穗期至50%呈显著正相关。千粒重与抽穗天数至50%之间的表型和基因型呈显著正相关,穗长次之。表型和基因型对产量的直接正向影响最大的是抽穗天数,其次是穗长和每穗实粒数。穗长对籽粒成熟日数(-0.167)、千粒重(-0.162)和株高(-0.09)均有显著负向间接效应。其余6个性状的直接影响太低,不被认为是重要的,而在通径分析中获得的其他间接影响的估计可以忽略不计。总的来说,旱稻在提高产量及其组成部分方面具有获得高响应的有利条件。
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引用次数: 0
Effect of growth regulators concentrations on in vitro multiplication of three elite sugarcane (Saccharum officinarum L.) Genotypes using shoot tip culture 生长调节剂浓度对三种优质甘蔗体外增殖的影响利用茎尖培养进行基因分型
Pub Date : 2022-02-24 DOI: 10.25081/jsa.2022.v6.7267
Gerema Amente, T. Feyissa
Conventional vegetative propagation of sugarcane generally has low multiplication rate and allows distribution of diseases. Micropropagation is the only practical means of achieving rapid, large-scale production of disease-free quality planting material. Experiments on shoot tip culture initiation and shoot multiplication were laid out in completely randomized design with 2x3x3 and 4x5x3 factorial treatment arrangements respectively. Data were subjected to analysis of variance (ANOVA) and significant means were separated using Duncan's multiple range tests. With regard to shoot multiplication, genotype Q200 showed a maximum of 13.59 shoots per explant with 5.83cm shoot length on a medium fortified with 2 mg/l BAP alone, while genotype Q217 produced a maximum of 15.28 shoots per explant with 5.37cm mean shoot length on a medium supplied with 2.0 mg/l BAP and 0.25 mg/l kinetin. Likewise, Co-0238 produced a maximum of 13.56 shoots per explant with a mean shoot length 6.50 cm on medium fortified with 1.5 mg/l BAP + 0.5mg/l kinetin.
甘蔗常规无性繁殖繁殖繁殖率低,病害易传播。微繁繁殖是实现快速、大规模生产无病优质种植材料的唯一实用手段。试验采用完全随机设计,分别采用2 × 3x3和4 × 5x3因子处理。数据进行方差分析(ANOVA),显著性均值采用Duncan多重极差检验分离。在芽增殖方面,Q200基因型在添加2 mg/l BAP的培养基上,每个外植体最多能产生13.59个芽,芽长5.83cm; Q217基因型在添加2.0 mg/l BAP和0.25 mg/l动素的培养基上,每个外植体最多能产生15.28个芽,芽长5.37cm。同样,Co-0238在添加1.5 mg/l BAP + 0.5mg/l kinetin的培养基上,每个外植体最多能产生13.56个芽,平均芽长6.50 cm。
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引用次数: 1
Soil physicochemical properties in respect to plant health in Ganodermainfested oil palm plantation 以灵芝为主的油棕种植园土壤理化性质对植物健康的影响
Pub Date : 2022-02-24 DOI: 10.25081/jsa.2022.v6.7446
Shervinia Dwi Ayundra, S. Suwandi, S. Herlinda, H. Hamidson, R. Wandri, D. Asmono
Basal stem rot caused by Ganoderma boninense is a major disease of oil palm in Indonesia. Environmental factors associated with disease can be used as the basis for developing disease control. This study aims to describe the physical and chemical properties of oil palm plantations land mineral soil land that is infested with basal stem rot. Soil composite samples were randomly collected from diseased and healthy palm circle from an oil palm plantation in Mesuji, South Sumatra. The results showed that the texture and nutrient status of diseased and healthy plants were not significantly different, namely they had the same low analytical values ​​in terms of the content of N, C-organic, CEC, Mg, Ca, Na, and K2O. The ratios of C/N, exchangeable K and exchangeable acidity in healthy and diseased soils were both moderate to high. The content of available P and total P was very high (43−134 ppm) and was found to be higher in the soil of diseased plants than in healthy plants. These results indicated that high availability of P nutrients may favor G. boninense infection in oil palm plantation.
根茎腐病(Ganoderma boninense)是印尼油棕的主要病害。与疾病相关的环境因素可作为制定疾病控制的基础。本研究旨在描述基底茎腐病发生的油棕种植园土地矿质土土地的物理化学性质。在南苏门答腊岛Mesuji的一个油棕种植园的病棕榈圈和健康棕榈圈中随机采集土壤复合样品。结果表明,病株和健康株的质地和营养状况无显著差异,即在N、c -有机、CEC、Mg、Ca、Na和K2O含量方面具有相同的低分析值。健康土壤和病害土壤的碳氮比、交换性钾和交换性酸度均为中高。病株土壤速效磷和全磷含量很高(43 ~ 134 ppm),且病株土壤速效磷和全磷含量高于健康株。这些结果表明,高磷养分的有效度可能有利于油棕田牛肉牛侵染。
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引用次数: 2
Performance evaluation of improved mung bean (Vigna radiata (L.) Wilczek) varieties at low moisture areas East Shewa, Oromia, Ethiopia 改良绿豆(Vigna radiata, L.)的性能评价威尔切克)品种在低湿度地区东谢瓦,奥罗米亚,埃塞俄比亚
Pub Date : 2022-02-23 DOI: 10.25081/jsa.2022.v6.7289
T. Dinsa, Urgaya Balcha, Fayisa Benya, Mihratu Fufa
Mung bean is a useful crop in drier areas and has a good potential for crop rotation and relay cropping with cereals using residual moisture. The experiment was conducted at Adami Tulu Agricultural Research Center (ATARC), Lume and Dugda during 2018 and 2019 with the objective to identify adaptable and high yielder mung bean varieties for East Shewa Zone and similar agro ecologies. Four released mung bean varieties Shewa robit, Beroda, N-26 and Arkebe used as planting material. The experiment was laid down in Randomized Complete Block Design (RCBD) with three replications. The plot size was 1.8m × 2.5 m (4.5 m2) having 6 rows and a spacing of 0.30 m between rows and 50 cm between replication, 1 m between blocks. Data’s like  height (cm), number of pods per plant, number of seeds per pod, days to days to flowering, days to maturity, grain yield (kg ha-1), 100 seed weight (g) were collected and analyzed using SAS software.  The combined analysis of variance showed that there was significant variation at (P≤0.05 and P≤0.01) among the studied varieties, locations, and year main effect. There were also significant interaction effect on location by year, varieties by year and location by varieties by year for grain yield and other yield components. But non-significant on varieties by location for all traits except plant height and indicated those varieties were performed similarly across the locations.  Shewa Robit variety had a higher grain yield (1607.4 kg ha-1) followed by N-26 (1542 kg ha-1) and Beroda (1466.1 kg ha-1). While Arkebe Variety had a lower grain yield (893.4 kg ha-1) as compared with other varieties. Therefore Shewa Robit and N-26 were recommended for the study area and similar agro-ecologies.
绿豆在干旱地区是一种有用的作物,在利用剩余水分的谷物轮作和轮作中具有良好的潜力。该试验于2018年和2019年在阿达米图鲁农业研究中心(ATARC)、卢姆和杜格达进行,目的是为东舍瓦区和类似的农业生态环境寻找适应性强、高产的绿豆品种。选用4个已发布的绿豆品种Shewa robit、Beroda、N-26和Arkebe作为种植材料。试验采用随机完全区组设计(RCBD), 3个重复。小区面积为1.8m × 2.5 m (4.5 m2),有6行,行间距0.30 m,复制间距50 cm,块间距1 m。采用SAS软件采集植株高度(cm)、单株荚数、每荚种子数、开花天数、成熟天数、籽粒产量(kg ha-1)、百粒重(g)等数据进行分析。综合方差分析表明,各研究品种、地点和年份主效应间存在显著差异(P≤0.05和P≤0.01)。籽粒产量和其他产量构成要素在分地、分品种和分地之间也存在显著的互作效应。除株高外,其他性状在不同地理位置间的差异均不显著,表明不同地理位置间的差异相似。Shewa Robit品种籽粒产量最高(1607.4 kg ha-1),其次是N-26 (1542 kg ha-1)和Beroda (1466.1 kg ha-1)。而Arkebe品种籽粒产量较低(893.4 kg hm -1)。因此,在研究区和类似的农业生态环境中推荐使用Shewa Robit和N-26。
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引用次数: 1
Evaluation of selected semi-dwarf Tef (Eragrostis tef (Zucc.) Trotter) genotypes for yield and yield related traits 精选半矮秆Tef (Eragrostis Tef (Zucc.))的评价产量和产量相关性状的基因型
Pub Date : 2022-01-10 DOI: 10.25081/jsa.2023.v7.7629
Mahilet Tadesse, W. Kebede, Kidist Tolossa, T. Fikre, Yazachew Genet, Habte Jifar, S. Chanyalew, K. Assefa, S. Mitiku, C. Nigus, Z. Tadele
Tef is the major staple food crop for Ethiopia which is cultivated by more than 6.7 million smallholder farmers. As an indigenous cereal, it is well adapted to diverse climatic and soil conditions; however, its productivity is very low mainly due to susceptibility to lodging. The objective of this study was to identify stable, high yielding and lodging tolerant tef genotypes for moisture stress areas of the country. A total of twenty genotypes including standard and local checks were tested. The field experiment was conducted using a 2m x 2m area with a completely randomized block design at six locations (Debre Zeit, Minjar, Alemtena, Melkassa, Sirinka and Axum) during the 2019 and 2020 main cropping seasons. Data were taken on plot and individual plant basis on eight pheno-agro-morphological characters including days to heading, days to maturity, grain filling period, plant height, panicle length, lodging index, above-ground shoot biomass and grain yield. The combined analysis of variance showed that the mean squares due to genotypes, locations and genotype interactions were highly significant (P<0.01) for the tested eight agronomical and morphological traits evaluated. Based on the current result, 39% of the tested genotypes had a higher yield advantage over the standard check Boset variety. On the other hand, 44% of the genotypes showed higher yields than the local check. Therefore, the promising genotype for lodging tolerance needs further testing in the variety verification trial and those genotypes are used as representative materials to develop varieties, especially for lodging tolerance.
Tef是埃塞俄比亚的主要粮食作物,有670多万小农种植Tef。作为一种本土谷物,它很好地适应了各种气候和土壤条件;然而,它的生产力很低,主要是由于容易倒伏。本研究的目的是为我国水分胁迫地区鉴定稳定、高产和耐倒伏的tef基因型。共检测了20个基因型,包括标准检测和局部检测。在2019年和2020年主要种植季,采用完全随机区组设计,在6个地点(Debre Zeit、Minjar、Alemtena、Melkassa、Sirinka和Axum)采用2m × 2m的面积进行田间试验。以抽穗期、成熟期、灌浆期、株高、穗长、倒伏指数、地上部生物量和籽粒产量8个物候形态指标为基础,在小区和单株上进行数据采集。综合方差分析表明,8个农艺性状和形态性状的基因型、位置和基因型相互作用的均方差均极显著(P<0.01)。根据目前的结果,39%的测试基因型比标准检查Boset品种具有更高的产量优势。另一方面,44%的基因型的产量高于当地检查。因此,有潜力的抗倒伏基因型需要在品种验证试验中进一步检测,并将这些基因型作为培育品种特别是抗倒伏品种的代表材料。
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引用次数: 0
Control of browning in plant tissue culture: A review 植物组织培养褐变的控制研究进展
Pub Date : 2021-12-28 DOI: 10.25081/jsa.2021.v5.7266
Gerema Amente, Emiru Chimdessa
Browning is one of the severe problems in plant tissue culture that hampers successful in vitro propagation of plants especially woody and perennial plants. In order to control the browning problem, different efforts has been made in vitro such as presoaking of explants in antioxidant solution, incorporation of antioxidants into medium, culturing in the dark period and frequent subculturing of explants. Presoaking of explants in antioxidant solutions like polyvinylpyrolidone (PvP) and ascorbic acid (AC) is one of the most frequently used. Incorporation of antioxidants such as 0.2-0.5g/l PvP and 15-250mg/l ascorbic acid into MS medium is commonly used to control browning in different plants and explants followed by activated charcoal, citric acid, MES and AIP. Moreover, frequent sub culturing and incubation of explants in the dark period is the other alternative. This review article includes the study of previous and current research achievements in a comprehensive way on the different methods to control browning problem in plant tissue culture and suggests further optimization for successful control of browning when using the same or different crops as well explants.
褐变是植物组织培养中严重的问题之一,严重影响了植物特别是木本植物和多年生植物的离体繁殖。为了控制外植体褐变问题,在体外培养中采取了抗氧化剂溶液预浸、培养基中加入抗氧化剂、暗期培养和频繁继代培养等措施。外植体在PvP和AC等抗氧化溶液中预浸是最常用的方法之一。在MS培养基中加入0.2-0.5g/l PvP和15-250mg/l抗坏血酸等抗氧化剂,通常用于控制不同植物和外植体的褐变,其次是活性炭、柠檬酸、MES和AIP。此外,在黑暗时期频繁的继代培养和外植体孵育是另一种选择。本文综述了植物组织培养中控制褐变问题的不同方法的研究成果,并提出了进一步优化同种或不同作物及外植体控制褐变问题的方法。
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引用次数: 3
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Journal of Scientific Agriculture
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