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Improving the Mechanical Properties of Epoxy by Adding Sub-micron Cantaloupe Peel Fibers 加入亚微米哈密瓜皮纤维改善环氧树脂的力学性能
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2910
Rand B. Lutfi, W. H. Jassim
The use of bio-fruit waste has more attention in recent years because of the low cost of bio-fibers and the protection of the environment. In this study, the epoxy was reinforced with fruit residues (cantaloupe peel powder) in proportions (1%, 2%, 3%, 4%, 5%, 7.5%, and 10% by weight) as results of mechanical tests such as impact, hardness, flexural and compression.Adding sub microns particle size cantaloupe peels particles with a weight ratio of 7.5% improves the epoxy mechanical properties, like impact strength, hardness, flexural strength, and compression strength by 59.43%, 5.8%, 45.7%, and 118.2%, respectively.Using X-ray diffraction, the crystallite size ( D) of cantaloupe peel the powder was about (3 nm).In this research, Scanning electron microscopy was used to examine the morphology of the (epoxy/7.5 % cantaloupe peel powder) composite and to interpret the improvement in epoxy mechanical properties.
近年来,由于生物纤维的低成本和对环境的保护,生物水果废弃物的利用越来越受到人们的关注。在本研究中,以水果残留物(哈密瓜皮粉)按重量比例(1%、2%、3%、4%、5%、7.5%和10%)增强环氧树脂,作为冲击、硬度、弯曲和压缩等力学试验的结果。添加重量比为7.5%的亚微米级哈密瓜皮颗粒,可使环氧树脂的冲击强度、硬度、抗弯强度和抗压强度分别提高59.43%、5.8%、45.7%和118.2%。通过x射线衍射,哈密瓜皮粉末的晶粒尺寸(D)约为(3 nm)。本研究采用扫描电子显微镜对(环氧树脂/ 7.5%哈密瓜皮粉末)复合材料的形貌进行了研究,并解释了环氧树脂力学性能的改善。
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引用次数: 0
Studying the Classification of Texture Images by K-Means of Co-Occurrence Matrix and Confusion Matrix 基于共现矩阵和混淆矩阵的k -均值纹理图像分类研究
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2894
Haider S. Kaduhm, H. Abduljabbar
In this research, a group of gray texture images of the Brodatz database was studied by building the features database of the images using the gray level co-occurrence matrix (GLCM), where the distance between the pixels was one unit and for four angles (0, 45, 90, 135). The k-means classifier was used to classify the images into a group of classes, starting from two to eight classes, and for all angles used in the co-occurrence matrix. The distribution of the images on the classes was compared by comparing every two methods (projection of one class onto another where the distribution of images was uneven, with one category being the dominant one. The classification results were studied for all cases using the confusion matrix between every Two cases or two steps (two different angles and for the same number of classes). The agreement percentage between the classification results and the various methods was calculated.
本研究以Brodatz数据库中的一组灰度纹理图像为研究对象,采用灰度共生矩阵(GLCM)建立图像特征库,像素间距离为1单位,四个角度(0,45,90,135)。使用k-means分类器对图像进行分类,从2类到8类,并对共现矩阵中使用的所有角度进行分类。通过比较每两种方法(将一个类投影到另一个类上,其中图像分布不均匀,其中一个类别占主导地位)来比较图像在类上的分布。使用每两个案例或两个步骤(两个不同的角度和相同数量的类别)之间的混淆矩阵对所有案例的分类结果进行研究。计算了分类结果与各种方法的一致性百分比。
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引用次数: 3
First Record of Banded Garden Spider Argiope trifasciata Forskal, 1775 (Araneae: Araniedae) in Baghdad, Iraq 标题1775年在伊拉克巴格达首次记录的带状花园蜘蛛Argiope trifasciata Forskal(蜘蛛目:蜘蛛科)
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2971
Hala G . Noori, Z. N. Al-Azawii
This study presented the first recorded of the banded garden spider Argiope trifasciata and    described for the first time in Baghdad city, Iraq. The specimens were collected from Al-Rashidiya sub-district north of Baghdad city on 26/ November/ 2021 (7:00 am- 6:00 pm) from fruit gardens (orange and apricot trees) and agriculture lands (palm trees, crops of wheat and vegetables). The spider "Argiope trifasciata" species is a member of the orb-weaving spider genus. It belongs to the Araniedae family, widespread worldwide, and has four pairs of long legs covered with spines and eight eyes arranged in two rows. The females of Argiope trifasciata spiders are yellow and covered with silvery and shiny hairs on the carapace that are relatively low. The abdomen of described spider is usually oval and elongated with pale yellow and black stripes. The legs are yellowish brown with dark rings. 
本文报道了在伊拉克巴格达首次发现的带状花园蜘蛛Argiope trifasciata。标本于2021年11月26日(上午7点至下午6点)在巴格达市北部Al-Rashidiya街道的果园(橙树和杏树)和农田(棕榈树、小麦和蔬菜作物)采集。蜘蛛“Argiope trifasciata”种是圆织蜘蛛属的一员。它属于蜘蛛科,分布在世界各地,有四对覆盖着刺的长腿和两排排列的八只眼睛。Argiope trifasciata蜘蛛的雌性是黄色的,在相对较低的甲壳上覆盖着银色和闪亮的毛发。所描述的蜘蛛的腹部通常是椭圆形和细长的,有淡黄和黑色的条纹。腿是黄褐色的,有黑色的环。
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引用次数: 0
Preparation, Structural Characterization and Biological Activities of Curcumin-Metal(II)-L-3,4-dihydroxyphenylalanin (L-dopa) complexes 姜黄素-金属(II)- l -3,4-二羟基苯丙氨酸(L-dopa)配合物的制备、结构表征及生物活性
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2899
Hala M. Salh, T. AL-Noor
In the present work, a first-row divalent d-transition metal obtained from curcumin(Curc)  and L-3,4-dihydroxyphenylalanin(L-dopa)have been synthesized which their complexes and characterized by  C.H.N, conductance, spectral methods: FT-IR, Ultra–Visible. Magneto-chemical measurements, molar conductance ΛM (1×10−3 mol/L in DMSO):36- 0.84 ohm-1.cm2.mol-1 (non-electrolyte).The data shows that the complexes have the structure [M((II))-(Curc)-(L-dopa)] system. Electronic and magnetic data suggest an octahedral geometry for all complexes in which the (L-dopa) and curcumin act as bidentate ligands.Curcumin coordinated to the metal ions M (II) through the lone pair of electrons of oxygen in 2(C=O) groups. The (L-dopa) coordinated to M (II) as a mono negative bidentate ligand through the oxygen atom of the carboxylate and the (N), atom of the (-NH2) groups.   The general formula  was given for the prepared mixed ligand complexes as [M (Cur)(L-dopa)2 ].  M= Mn  (II), Fe (II),Co(II),Ni(II),Cu (II), Zn(II), Cd(II) and Hg(II).The ligands and their metal complexes were screened for their antimicrobial activity klebsiella pneumonie,and  Staphylococcus aureus, and Candida albicans. Metal chelates showed very good antimicrobial activity than their parent curcumin-and (L- dopa).  We have given the general formula for the prepared mixed ligand complexes as [M (Cur)(L-dopa)2].M= Mn  (II), Fe (II),Co(II)  ,Ni(II),Cu (II), Zn(II), Cd(II) and Hg(II).The ligands and their metal complexes were screened for their antimicrobial activity klebsiella pneumonie,and  Staphylococcus aureus, and Candida albicans. Metal chelates show very good antimicrobial activity than their parent Curcumin-and (L- dopa).
本文以姜黄素(Curc)和l -3,4-二羟基苯丙氨酸(L-dopa)为原料合成了一种第一排二价d-过渡金属,并通过电化学、电导、红外光谱、超可见光等方法对其进行了表征。磁化学测量,摩尔电导ΛM (1×10−3 mol/L的DMSO):36- 0.84欧姆-1 cm2。mol-1(非电解质)。数据表明,配合物具有[M((II))-(Curc)-(L-dopa)]体系结构。电子和磁数据表明,所有配合物的八面体几何形状,其中(左旋多巴)和姜黄素作为双齿配体。姜黄素通过氧的孤对电子在2(C=O)基团中与金属离子M (II)配位。(L-dopa)通过羧酸盐的氧原子和(-NH2)基团的(N)原子与M (II)配位为单负双齿配体。给出了所制备的混合配体配合物的通式为[M (Cur)(L-dopa)2]。M =锰(II)、铁(II),公司(II)、镍(II)、铜(II)、锌(II)、Cd (II)和Hg (II)。对这些配体及其金属配合物进行了抑菌活性筛选,检测其对肺炎克雷伯菌、金黄色葡萄球菌和白色念珠菌的抑菌活性。金属螯合物具有较好的抗菌活性,其亲本姜黄素和左旋多巴具有较好的抗菌活性。我们给出了所制备的混合配体配合物的通式为[M (Cur)(L-dopa)2]。M =锰(II)、铁(II),公司(II)、镍(II)、铜(II)、锌(II)、Cd (II)和Hg (II)。对这些配体及其金属配合物进行了抑菌活性筛选,检测其对肺炎克雷伯菌、金黄色葡萄球菌和白色念珠菌的抑菌活性。金属螯合物比其母体姜黄素和左旋多巴具有更好的抗菌活性。
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引用次数: 0
Exponentially Fitted Diagonally Implicit EDITRK Method for Solving ODEs 求解ode的指数拟合对角隐式EDITRK方法
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2883
Firas A. Fawzi, Nour W. Jaleel
This paper derives the EDITRK4 technique, which is an exponentially fitted diagonally implicit RK method for solving ODEs . This approach is intended to integrate exactly initial value problems (IVPs), their solutions consist of linear combinations of the group functions  and  for exponentially fitting  problems, with  being the problem’s major frequency utilized to improve the precision of the method. The modified  method EDITRK4 is a new three-stage fourth-order exponentially-fitted diagonally implicit approach for solving IVPs with functions that are exponential as solutions. Different forms of -order ODEs must be derived using the modified system, and when the same issue is reduced to a  framework of equations that can be solved using conventional RK approaches, numerical comparisons must be done. The findings show that the novel approach is more efficacious than previously published methods.
本文导出了EDITRK4技术,它是求解ode的指数拟合对角隐式RK方法。该方法旨在精确地整合初值问题(ivp),其解决方案由组函数的线性组合组成,并用于指数拟合问题,作为问题的主要频率,用于提高方法的精度。修正后的EDITRK4方法是求解以指数函数为解的ivp的一种新的三阶段四阶指数拟合对角隐式方法。必须使用改进的系统推导出不同形式的-阶ode,当相同的问题被简化为可以使用传统RK方法求解的方程框架时,必须进行数值比较。研究结果表明,这种新方法比以前发表的方法更有效。
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引用次数: 1
Experimental Investigation on Adsorption of Methyl orange Using eggshells as adsorbent Surface 以蛋壳为吸附剂表面吸附甲基橙的实验研究
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2890
Hareer A. Al Nasir, S. S. Mohammed
The role of residues in the adsorption process for removing contaminants from their aqueous solution was highlighted in this study. The adsorption capacity of eggshells were used to remove the methyl orange dye from its aqueous solution. The highest dye adsorption was found to range between (62.30% to 62.33%). The results of using adsorption isotherms (Freundlich, Langmuir, and Temkin) have been revealed that the Freundlich model was followed and that the Langmuir model did not match, as well as the partial applicability of Temkin's model at temperatures (298,308,318) K. The process of adsorption is a physical one. Three kinetic models of the adsorption process were also used, with the results demonstrating the applicability of the pseudo-second-order model. In this study, the thermodynamic functions were estimated using the value of the enthalpy ΔH°, which was negative and equal to (-4.7685 KJ/mole), The process was discovered to be exothermic, and the entropy ΔS° value was also negative, equaling (- 11.5100 J/mole.K), indicating a decrease in the randomness of adsorption when added to the Gibbs free energy ΔG°, indicating that the reaction occurred spontaneously. 
在本研究中,残留物在其水溶液中去除污染物的吸附过程中的作用得到了强调。利用蛋壳的吸附能力对甲基橙染料进行脱除。对染料的最高吸附在62.30% ~ 62.33%之间。利用吸附等温线(Freundlich、Langmuir和Temkin)的结果表明,遵循Freundlich模型,与Langmuir模型不匹配,以及Temkin模型在(298,308,318)k温度下的部分适用性。采用了三种吸附动力学模型,结果表明了拟二阶模型的适用性。在本研究中,利用焓ΔH°的值估计热力学函数,该值为负,等于(-4.7685 KJ/mol),发现该过程为放热过程,熵ΔS°的值也为负,等于(- 11.5100 J/mol . k),说明加入吉布斯自由能ΔG°后,吸附的随机性降低,说明反应是自发发生的。
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引用次数: 1
The Water Area of Sawa Lake as Derived from Land Surface Temperature and Remote Sensing Data 基于地表温度和遥感数据的萨瓦湖水域面积
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2873
Haidar A. Radeef, I. Abdulameer
Sawa Lake is one of the unique lakes in Iraq. It is located in the southwestern part of Iraq. It is one of the closed lakes, as no surface water source works to feed the lake. The lake feeds on groundwater. The source of this groundwater is the Dammam Basin. During the past ten years, The lake has had many changes, which led to a decrease in water levels. This also led attention to study of the causes of these changes. Many types of research were presented in the study of the state of the lake. This research used remote sensing images from Landsat 8 OLI to monitor the changes during 2020-2021 by applying the NDWI equation to extract water area from image data. The results of the areas were obtained from a special report by Normalized Difference Water Index (NDWI). Then, the lake surface water temperature was derived from Landsat 8 thermal bands (TIRS). The lake water area and temperatures increased in summer and decreased in winter and autumn. Then, it is not the main reason for the change in the lake water area. The levels of the lake water rise during the dry period and decrease during the rainy period. The natural factors have little influence in reducing the lake water. It is attributed to human factors, groundwater depletion, and a reduction in water supply from the Dammam Basin, which feeds the lake.
萨瓦湖是伊拉克独特的湖泊之一。它位于伊拉克西南部。它是一个封闭的湖泊,因为没有地表水的来源工程来喂养湖泊。这个湖以地下水为水源。地下水的来源是达曼盆地。在过去的十年里,这个湖发生了许多变化,导致水位下降。这也引起了人们对这些变化原因的研究。在对湖泊状况的研究中,提出了许多类型的研究。本研究利用Landsat 8 OLI遥感影像,应用NDWI方程从影像数据中提取水域面积,监测2020-2021年的变化。这些地区的结果来自一份特别报告,采用归一化差水指数(NDWI)。然后利用Landsat 8热带(TIRS)反演湖泊地表水温。湖水面积和温度夏季呈上升趋势,冬季和秋季呈下降趋势。因此,它不是湖泊水域变化的主要原因。枯水期湖水水位上升,雨季水位下降。自然因素对湖泊水量的减少影响不大。这是由于人为因素、地下水枯竭以及达曼盆地供水量减少造成的。达曼盆地是该湖泊的水源。
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引用次数: 0
The Role of the Vegetative Part in Some Plant Species to Uptake and Accumulate Lead Element from Polluted Air (an Applied Study in Baghdad /Karkh in Iraq) 某些植物营养部分对污染空气中铅元素的吸收和积累作用(在伊拉克巴格达/Karkh地区的应用研究)
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2949
N. A. Khayoon, I. M. Al-Salman
The current study was conducted to test the efficiency of the vegetative part (plant leaves) of plant species of shrubs and trees involved in forming semi-artificial vegetation in the city of Baghdad, Karkh, in the uptake and accumulating the lead element that pollutes the air in the city atmosphere. Five plant sampling sites were selected: Al-Kadhimiyah, Al-Mansour, Al-Ma'aml (Al-Salam district), Al-Adl, and Al-Ameriya district intersections (Al-Seklat), and symbols were given (A, B, C, D, E) respectively. The spread and distribution of plants vary in terms of human activities and pollution levels, affecting the five sites that recorded more than 20 species. For a real comparison between plant efficiency and the effect of the nature of the region, species of recurrent and non-recurring shrubs and trees were selected in their presence at the study sites and included (Conocarpus lancifolius, Ziziphus spina christi, Eucalyptus sp., Albizia lebbeck), and non-recurring (Nerium oleander, Dodonaea viscosa, Phoenix dactylifera, Olea europaea, Myrtus communis, Ficus nitida, Citrus aurantium). The study's results showed a variation in the ability of plant species in lead accumulators. The first site of the plants (Conocarpus lancifolius, Ziziphus spina christi, Albizia lebbeck, Eucalyptus sp., Nerium oleander, Dodonaea viscosa, Phoenix dactylifera, Olea europaea, Myrtus communis, Ficus nitida, and Citrus aurantium) was recorded at (0.46, 0.56, 0.36, 0.55, -, 0.68, -, -, 0.33, 0.29, 0.84) respectively. The second site of the same plants was (0.74, below the detection limit, 0.25, 0.57, -, -, 0.16, -, -, 0.31, -) respectively. The third site was (0.95, 0.65, 0.832, 0.831, 0.86, 1.02, -, -, -, 0.436, -, 0.532), respectively. The fourth site was (0.34, 0.95, 0.48, 0.40, -, 0.19, -, -, -, -), respectively. The fifth site was (0.48, 0.50, 0.49, 0.41, -, -, -, 1.45, -, -, -, -) ppm, respectively. The current study was conducted from October 2021 to May 2022
目前进行的研究是为了测试在伊拉克巴格达市形成半人工植被的灌木和树木植物种类的营养部分(植物叶片)在吸收和积累污染城市大气中空气的铅元素方面的效率。选取Al-Kadhimiyah、Al-Mansour、Al-Ma'aml (Al-Salam区)、Al-Adl和Al-Ameriya区路口(Al-Seklat) 5个植物采样点,分别标记为A、B、C、D、E。植物的传播和分布因人类活动和污染程度而异,影响了五个记录了20多种植物的地点。为了真正比较植物效率和区域性质的影响,选择了研究地点存在的周期性和非周期性灌木和乔木物种,包括(Conocarpus lancifolius, Ziziphus spina christi, Eucalyptus sp., Albizia lebbeck)和非周期性(Nerium oleander, Dodonaea viscosa, Phoenix dactylifera, Olea europaea, Myrtus communis, Ficus nitida, Citrus aurantium)。研究结果表明,不同种类的植物在铅蓄积器中的吸收能力存在差异。第1位分别为Conocarpus lancifolius、Ziziphus spina christi、Albizia lebbeck、Eucalyptus sp.、Nerium oleander、Dodonaea viscosa、Phoenix dactylifera、Olea europaea、Myrtus communis、Ficus nitida和Citrus aurantium,分别为(0.46、0.56、0.36、0.55、-、0.68、-、-、0.33、0.29、0.84)。同一植物的第二位点分别为(0.74,低于检测限,0.25,0.57,-,-,0.16,-,-,0.31,-)。第三个位点分别为(0.95、0.65、0.832、0.831、0.86、1.02、-、-、-、0.436、-、0.532)。第四个位点分别为(0.34,0.95,0.48,0.40,-,0.19,-,-,-,-)。第五个网站(0.48,0.50,0.49,0.41 , -, -, -, 1.45 , -, -, -, -) 分别ppm。目前的研究是从2021年10月到2022年5月进行的
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引用次数: 1
The Role of TNF-α and Total IgE in Pathogenicity of Iraqi Fuel Stations Workers with Allergic Rhinitis TNF-α和总IgE在伊拉克加油站工作人员变应性鼻炎致病性中的作用
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2994
M. Q. Ahmed, T. Hussein
Allergic Rhinitis (AR) is a chronic immune system inflammation that occurs when the body overreacts to antigens in the environment (triggers) and produces a variety of symptoms in the nasal mucosa and paranasal sinuses due to the release of many interleukins and cytokines.We investigated the influence of the allergen on serum levels of TNF-α and Total IgE in patients with AR and their function in initiating allergic rhinitis in Iraqi petrol station workers in Baghdad. One hundred individuals with allergic rhinitis and thirty healthy workers between the ages of 20 and 59 were examined. For immunological testing, sandwich ELISA was used to evaluate serum levels of TNF-α and Total IgE. A complete blood cell count (CBC) was used for hematological testing. Findings showed a positive connection between TNF-α and Total IgE, with a correlation of (r=0.32). The TNF-α binary logistic regression analysis (BLR) was conducted to be highly significant (B = 0.02, OR = 1.02, p =0.065), viewing that an increase of one unit in TNF-α increases the odds of belonging to the AR group. TNF-α had the highest area under the curve (AUC) compared to other markers, with a value of (AUC = 0.995), indicating that it is an excellent predictor and strong marker for AR, with a sensitivity and specificity of 94% and 100%, respectively. After our research, we reached the significance of the study from the clarity of the role of tumor necrosis factor-alpha in the disease and its development and the coincidence of its increase with immunoglobulin E and considering. It is also a diagnostic marker of the disease.
过敏性鼻炎(AR)是一种慢性免疫系统炎症,当机体对环境中的抗原(触发器)反应过度时,由于许多白细胞介素和细胞因子的释放,在鼻黏膜和鼻窦产生各种症状。我们研究了变应原对AR患者血清TNF-α和总IgE水平的影响及其在巴格达伊拉克加油站工作人员过敏性鼻炎发病中的作用。对100名过敏性鼻炎患者和30名年龄在20至59岁之间的健康工人进行了调查。免疫检测采用夹心ELISA法检测血清TNF-α和总IgE水平。全血细胞计数(CBC)用于血液学检测。结果显示TNF-α与总IgE呈正相关,相关系数为(r=0.32)。TNF-α二元logistic回归分析(BLR)结果极显著(B = 0.02, OR = 1.02, p =0.065),表明TNF-α每增加1个单位,属于AR组的几率就会增加。与其他标志物相比,TNF-α的曲线下面积(AUC)最高(AUC = 0.995),表明TNF-α是AR的良好预测因子和强标记物,敏感性和特异性分别为94%和100%。经过我们的研究,我们从肿瘤坏死因子- α在疾病及其发展中的作用的明确以及其升高与免疫球蛋白E的一致性和考虑,得出了本研究的意义。它也是该疾病的诊断标志。
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引用次数: 0
Anti-tumor Activity of Plantago lanceolata Aqueous Extract In Vitro and Genotoxicity by Micronucleus Assay In Vivo 车前草水提物体外抗肿瘤活性及体内微核遗传毒性研究
Pub Date : 2023-01-20 DOI: 10.30526/36.1.2953
Eman Ali H. Al-Mosawie, Worood K. S. Al-Maliky
The study is designed to evaluate the effect of the aqueous extract of the P. lanceolata plant, as well as to know the effect of the drug CCl4 on the formation of micronucleus in vivo 48 female albino mice. In the study mice were separated into eight groups treated intraperitoneally for seven day first group Negative control, second positive control( CCl4 0.02%), third group aqueous extract (250 mg/kg), fourth group  aqueous extract (500 mg/kg), fifth group (CCl4 0.02%) plus aqueous extract (250 mg/kg), sixth group (CCl4 0.02%) plus aqueous extract (500 mg/kg), seventh group aqueous extract (250 mg/kg) plus (CCl4 0.02%), and eighth group aqueous extract (500 mg/kg) plus (CCl4 0.02%). The genetic-cellular aspect involved measuring the coefficient of micronucleus formation in bone marrow cells in mice treated with CCl4 and plant aqueous extract. The results showed that the treatment of mice with the drug led to a rise in the coefficient of micronucleus formation compared to the negative control group. In addition, it showed the plant's ability to reduce the drug CCl4 effect in the totals of overlaps between the plant extract and the drug at the concentrations used for the plant 250 and 500 μg/ml and reduce the formation of micronucleus.The cellular toxicity of the plant’s aqueous extract on the liver cancer cell line was assessed in HepG2 (liver cancer cell line) and the WRL68 (hepatic human cell line) using concentrations (25, 50, 100, 200, and 400 μg /ml) from the plant’s aqueous extract on the HepG2 liver cancer cell line. The results showed a decrease in cell viability depending on aqueous extract concentration. The vitality of cancer cells decreased with the increase in concentration; the viability of the aqueous extract of the plant on cancer cells reached the minimum at concentration 400 μg/ml 45.34±4.44, while it reached the maximum when concentration  25 μg/ml 84.53±2.41.
本研究旨在评价杉木水提物对48只雌性白化小鼠体内微核形成的影响,并了解药物CCl4对微核形成的影响。将小鼠分为8组,腹腔注射7 d,第一组阴性对照组、第二组阳性对照组(CCl4 0.02%)、第三组水提取物(250 mg/kg)、第四组水提取物(500 mg/kg)、第五组(CCl4 0.02%)加水提取物(250 mg/kg)、第六组(CCl4 0.02%)加水提取物(500 mg/kg)、第七组水提取物(250 mg/kg)加(CCl4 0.02%)、第八组水提取物(500 mg/kg)加(CCl4 0.02%)。遗传-细胞方面涉及测量小鼠骨髓细胞微核形成系数的CCl4和植物水提取物处理。结果表明,与阴性对照组相比,用该药治疗小鼠导致微核形成系数升高。此外,在植物使用浓度为250 μg/ml和500 μg/ml时,植物提取物与药物之间的总重叠量显示出降低药物CCl4效应的能力,并减少微核的形成。在HepG2(肝癌细胞系)和WRL68(人肝细胞系)中,采用浓度分别为25、50、100、200和400 μg /ml的水提物对肝癌细胞系进行细胞毒性评价。结果表明,细胞活力随水提物浓度的变化而降低。癌细胞活力随浓度的增加而降低;水提物对癌细胞的活性在400 μg/ml时最低(45.34±4.44),在25 μg/ml时最高(84.53±2.41)。
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引用次数: 0
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