Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao最新文献

Objective: To aim at demonstrating whether cationic liposome-mediated antisense c-myb oligonucleotide(LipoAON) can inhibit the growth of C6 glioma by intravenous injection.

Methods: Intracerebral C6 glioma cells were implanted into the left caudal nucleus of forty-eight male Wistar rats. There were four groups: LipoAON(n = 12), antisense c-myb oligonucleotide (AON; n = 12), cationic liposome (Lipo; n = 12), and normal saline (NS; n = 12). Six days after tumor implantation, the above-mentioned drugs were injected into the right femoral veins of the rats respectively. Two days later, the same drugs were injected into the left femoral veins. The appetite, motor and weight of every animal were closely observed during the whole experiment. Six rats of each group were respectively killed 4 days and 10 days after the end of administration. The weight change, pathologic examination and immunohistochemical analysis of c-myb expression of the tumor were completed.

Results: In LipoAON group, the growth of the tumors was significantly inhibited in a short time after treatment and c-myb expression was down-regulated. But in the AON group and Lipo group, the growth of the tumors was not inhibited and c-myb expression was not down-regulated, compared with that in NS group. The inhibitory effect of LipoAON on the tumors rapidly declined with time and c-myb expression was again up-regulated.

Conclusion: 1. Cationic liposome (LipofectAMINE) as transfection vehicle makes c-myb easily penetrate BBTB and enter the tumor. The technique is simple, safe, highly effective for the transfection of c-mybAON; 2. LipoAON has marked inhibitory effect on the growth of C6 glioma. The AON technical method for inhibiting the expression of c-myb oncogene has a research perspective in the treatment of glioma; 3. The inhibitory effect of LipoAON on the growth of glioma declines with time. The question about how to make c-myb AON have highly effective, sustained and stable expression in the tumor still requires further research.

Objective: To direct the relationship between the activity of glutathione S-transferases(GSTs) and susceptibility to trinitrotoluene cataract (TC).

Methods: KMSL method was used to test the activity of GSTs in 154 cases of TC(group A), 41 cases of non-trinitrotolune cataract (group B), and 40 normal subjects (group B).

Results: The activity of GSTs in group TC was significantly lower than that in the two control groups (P < 0.01). The differences in GSTs activity were highly significant between the four stages of TC (P < 0.01), in which the sequence of GSTs activity levels from high to low was the stage of observation, stage I, stage II, and stage III. Compared to the persons with gst mu gene, the persons without gst mu gene had significantly lower GSTs activity (P < 0.01). It was found that the GSTs activity of the persons without gst mu gene in TC group was significantly lower than that of the persons without gst mu gene in the two control groups (P < 0.01), but the differences in GSTs activity between the persons with gst mu gene among the three groups were not significant (P > 0.05). The most noticeable correlation was observed between GSTs activity gst mu gene deletion (r = 0.702, P < 0.01).

Conclusion: TC and its stages are closely correlated with the activity of GSTs.

Objective: To investigate the relationship between preterm labour and the ratio of nitric oxide to prostaglandin E2 in uterine tissues.

Methods: Pregnant mice were given lipopolysaccharide(LPS), LPS + DETA/NO, LPS + DETA or saline solution respectively by intraperitoneal injection on day 16 of gestation. The delivery time of each mouse was recorded. Four separate groups of mice were given treatment as described. And they were killed at 12 hours after that. Serum progesterone, uterine production of nitrite and PGE2 were measured.

Results: Uterin production of nitrite and PGE2 in LPS-treated mice was significantly higher than that in control mice. In contrast the ratio of nitrite to PGE2 decreased. Accompanied by the fall of serum progesterone level DETA/NO rather than DETA could partially reversed the changes.

Conclusion: Infection can induce preterm labor by altering the ratio of nitric oxide to PGE2. Nitric oxide can prevent the decrease of nitric oxide to PGE2 ratio possibly by attenuating the fall of serum progesterone. And it can prevent LPS-induced preterm labor.

Objective: To examine the immunophenotype of adult acute leukemia and analyze its clinical significance.

Methods: Immunophenotypes were examined by direct immunofluorescence method and flowcytometry in 69 de novo AL.

Results: 1. The incidence of myeloid antigen expression in 29 adult ALL was 30%. Among them, CD13 was 10.3% and CD33 was 20.7%, no difference was found in expression of myeloid antigens between B-ALL and T-ALL (P > 0.05). Myeloid antigens were expressed much more higher in CD34 positive ALL than in CD34 negative ALL (77.8% vs 13.3%, P = 0.036). CR rate in myeloid antigen positive ALL was lower than in myeloid antigen negative ALL (33.3% vs 80%, P = 0.0203). 2. The incidence of lymphoid antigen expression in 40 adult AML was 30%. Among them, CD7 was 15%, CD19 12.5% and CD2 2.5%. CD7 was mainly expressed in M1 and M2 subtypes. Lymphoid antigens were expressed higher in CD34 positive AML than in CD34 negative AML (61.1% vs 4.5%, P = 0.000125). Though CR rate was lower in lymphoid antigen positive AML than in lymphoid antigen negative AML, no significant difference between them was noted (50% vs 71.4%, P = 0.126).

Conclusion: Aberrant antigen expression in adult acute leukemia is about 30%. Antigen aberrant is much higher in CD34 positive AL than in CD34 negative AL. The CR rate of antigen aberrant AL is lower than that of usual AL.

Objective: Using molecular techniques, we typed 2 short tandem repeat (STR) loci on 21 chromosome to establish the method for rapid and accurate diagnosis of trisomy 21.

Methods: Genomic DNA samples from 50 individuals diagnosed previously by karyotype as trisomy 21 and 40 children with severe mental retardation (IQ < 50) suspected of trisomy 21 were analyzed for 2 short tandem repeat loci on 21 chromosome, D21S1435 and D21S2055. Typing was carried out after polymerase chain reaction (PCR) and silver staining. The trisomy was identified by the number of alleles: 3 alleles bands whose density is same, two alleles bands with one obvious higher density compared to the other and one allele band whose density is three times than the normal control.

Results: All of the complete trisomy 21 were detected by this method; the parental source was easily determined.

Conclusion: This method for diagnosing trisomy 21 is rapid and accurate.

Objective: To improve the primary cell culture methods of colorectal adenomas.

Methods: The methods of the in vitro primary cell culture of colorectal adenomas, mainly the method for taking and preparing specimen, the methods of culture conditions and cells purification, were considered again and improved in this study.

Results: Of the 32 cases, of polyp, 26 were successfully cultured and 6 failed. The culture lasted 5-23 days with 50%-95% viscosity of wells. By means of immuno-histochemical staining and electromicroscopy, the cultured cells were proved to be of epithelial origin.

Conclusion: The primary culture methods of adenoma cells have been improved, but further study on the serial transfer culture cell lines is most needed.

Objective: To assess the effect of Astragalus membranaceus injection (AMI) on megakaryocyte hematopoiesis in anemic mice and explore its mechanism.

Methods: Anemic models of mice were randomly divided into two groups: treatment group and anemic control group. Intraperitoneal doses of AMI (20 mg/(ml.20 g) q.d x 6) were given to the treatment group, and equal doses of physiological saline were given to the anemic control group. On days 8, 11 and 14 after treatment, blood platelet and bone marrow cells were determined, and the numbers of CFU-Meg (colony forming unit-megakaryocyte) and Meg-CSA (megakaryocyte colony-stimulating activity) were determined by using technique of hematopoietic progenitor cells culture in vitro.

Results: Serum Meg-CSA of the treatment group was significantly higher than that of the anemic control group. The abovementioned indices of the treatment group recovered to normal by day 11, which was markedly earlier than the day of recovery observed in the anemic control group.

Conclusion: AMI can increase serum Meg-CSA of anemic mice and accelerate the recovery of megekaryocyte hematopoiesis after bone marrow suppression.

Objective: To investigate the effect of Danggui Buxue Decoction (DBD) on proliferation and expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs).

Methods: After treating HUVECs with various concentrations of DBD, we analyzed the proliferation phase of HUVECs and the expression of ICAM-1 using flow cytometry.

Results: It was found that DBD could promote the proliferation of HUVECs and the expression of ICAM-1. The expression of CD54 (ICAM-1) was significantly increased in the experiment groups as compared with that in the control group (P < 0.05). In addition, cell cycle analysis revealed more synthetic period cells in the experiment groups than in control group (P < 0.05).

Conclusion: DBD can promote the proliferation of HUVECs and Increase the expression ICAM-1.

Objective: To build the normal ranges and regression equations of peak expiratory flow (PEF) in normal young, middle-aged and old persons.

Methods: PEF, FVC, V75, V50, V25 were measured in 393 normal young, middle-aged and old persons with the youngest aged 20 years and the eldest 84 years. Regression equations of PEF were built for younger group (20 yr-49 yr) and elder group (50 yr-84 yr) respectively.

Results: PEF, FVC, V75, V50, V25 were decreased with increase of age. The regression equations showed that both in younger and elder groups, PEF was negatively correlated with age in male (P < 0.0001-0.002, r = 0.32-0.39) and positively correlated with FVC in female (P < 0.0001-0.0005, r = 0.32-0.59). PEF was highly correlated to V75 with r = 0.87-0.94 in male and r = 0.93-0.96 in female. The coefficient of variation (CV) of PEF was in normal range (16%-24%) for all age groups except male and female above 70 years old with CV of 30% and 27% respectively. This confirmed the data was reliable.

Conclusion: The normal ranges and regression equations of PEF constructed as a result of this study for normal humans of 20-84 years old can be put to use in clinical work and public health.

Objective: To investigate the effect of leukotriene antagonist (zafirlucast) on the parameters of dose-response curve (DRC) obtained after methacholine challenge test.

Methods: A therapy trial was conducted with the use of zafirlucast in 20 cases of cough variant asthma (CVA) and 20 cases of asthma. Placebo was used in 4 cases of CVA and 10 cases of asthma for comparison with the two therapy groups respectively. Before and after the zafirlucast treatment a series of parameters of DRC were obtained after the methacholine challenge test and were calculated. These included the response threshold (PC35sGaw), the dose-response slope (DRS), the ratio of the area under the curve to the logarithm of the maximal concentration (AUC/1 g[Cmax]), and the maximal response.

Results: Zafirlucast significantly increased the PC35sGaw in the CVA and asthma groups. It conspicuously decreased all the parameters of DRS and AUC/1 g[Cmax] except for maximal response in the cases of CVA, and it had no significant effects on all the parameters of DRS and AUC/1 g[Cmax] except for PC35sGaw in the cases of asthma.

Conclusion: Short-term use of Zafirlucast in asthmatic patients could improve the parameters of dose-response curve obtained after the methacholine challenge test.