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Detection of tox A gene in Pseudomonas aeruginosa that isolates from different clinical cases by using PCR. 不同临床病例铜绿假单胞菌毒素A基因的PCR检测。
Pub Date : 2018-04-23 DOI: 10.30526/2017.IHSCICONF.1767
R. M. Al-Shwaikh, A. F. Alornaaouti
Current study obtained (75) isolate of Pseudomonas aeruginosa collected from different cases included : 28 isolates from otitis media, 23 isolates from burn infections, 10 isolates from wound infections, 8 isolates from urinary tract infections and 6 isolates from blood, during the period between 1/9/2014 to 1/11/2014        The result revealed that the tox A gene was present in 54 isolates (72%) of Pseudomonas aeruginosa. The gel electrophoresis showed that the molecular weight of tox A gene was 352 bp. The result shows 17 isolates (60.71%) from otitis media has tox A gene, 18 isolates (78.26%) from burn followed by 8 isolate (80%) from wound infection and 5 isolates (62.5%) from urinary tract infection , finally 6 isolates (100%) from blood have this gene.
从2014年1月9日至2014年1月11日收集的铜绿假单胞菌共75株,其中:中耳炎28株,烧伤23株,伤口10株,尿路8株,血液6株。结果显示,54株铜绿假单胞菌(72%)存在tox A基因。凝胶电泳结果表明,毒素A基因分子量为352 bp。结果中耳炎17株(60.71%)含tox A基因,烧伤18株(78.26%),伤口感染8株(80%),尿路感染5株(62.5%),血液6株(100%)含该基因。
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引用次数: 5
Antimicrobial susceptibility of Enterococcus spp. Isolated from different clinical sources in Kirkuk provency 基尔库克省不同临床来源分离的肠球菌的抗菌药物敏感性
Pub Date : 2018-04-23 DOI: 10.30526/2017.IHSCICONF.1777
Hajir Ali Shareef, Sharazad N Abdullah
One of the most important problems confronts hospitals is the strains emergence  of Enterococcus spp. with multiple resistance to antibiotics, which propel researchers to modify or produce new antibiotics or combination between two antibiotics so that to be more effective against Enterococcus . This study was aimed to susceptibility some of local Enterococcus spp. Isolates with of 21 antibiotic using  disc diffusion method. The results showed absolute resistant 100% toward (Cephalexin , Gentamycin , Amikacin ,Erythromycin and Nalidixic acid), while showed a high sensitivity toward (Vancomycin and Impenem ) at percentage of 92.3% for each . Also highly inhibitory activity were observed by using penicillins antibiotics groups against most Enterococcus  isolates . which contribute to that non of the isolates showed it is ability to produce beta – lactamase enzymes by iodometric tube method. Also susceptibility to some new and synergetic antibiotic like Gentamicin High level(synergy), Streptomycin High level (synergy), Linezolid, Tigecycline, Levofloxacin, Quinupristin /Dalfopristin was conducted by Vitek-2 system. the results showed the absolute sensitivity (100%) of isolates toward ( Linezolid and Tigecycline).  All isolates showed multiple –resistant prescription to antibiotics , the number of antibiotics that every isolates resisted range between 6-12 antibiotic .
肠球菌对抗生素出现多重耐药是医院面临的最重要问题之一,这促使研究人员修改或生产新的抗生素或两种抗生素联合使用,以更有效地对抗肠球菌。采用圆盘扩散法对部分局部肠球菌分离株对21种抗生素的敏感性进行了研究。结果对头孢氨苄、庆大霉素、阿米卡星、红霉素和钠利地酸的绝对耐药率为100%,对万古霉素和伊培南的绝对敏感率为92.3%。用青霉素类抗生素组对大多数分离的肠球菌具有较高的抑制活性。结果表明,用碘量管法测定的分离菌株均未表现出产生β -内酰胺酶的能力。Vitek-2系统对庆大霉素高剂量(协同)、链霉素高剂量(协同)、利奈唑胺、替加环素、左氧氟沙星、奎奴普汀/达福普汀等新型协同抗生素进行敏感性分析。结果表明,菌株对利奈唑胺和替加环素的绝对敏感性均为100%。所有分离株均表现出对抗生素的多重耐药处方,每个分离株的耐药药数在6 ~ 12种之间。
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引用次数: 0
Comparison of Features Extraction Algorithms Used in the Diagnosis of Plant Diseases 植物病害诊断中特征提取算法的比较
Pub Date : 2018-04-23 DOI: 10.30526/2017.ihsciconf.1785
M. A. Hussein, Amel H. Abbas
The detection of diseases affecting plant is very important as it relates to the issue of food security, which is a very serious threat to human life. The system of diagnosis of diseases involves a series of steps starting with the acquisition of images through the pre-processing, segmentation and then features extraction that is our subject finally the process of classification. Features extraction is a very important process in any diagnostic system where we can compare this stage to the spine in this type of system. It is known that the reason behind this great importance of this stage is that the process of extracting features greatly affects the work and accuracy of classification. Proper selection of the right features leads to high accuracy in the system diagnostics and vice versa. The proposed system collect images of different crop (Rice, cotton and tomato) disease, we will enter the images of cropping them , then Re-size the images to fixed size, then improve the image through Fuzzy histogram equalization (FHE) , then perform image segmentation  using color based K-means  and finally compare the methods of features  extraction (Percentage of Leaf Area Infected (PI),Texture-Based Features, Color Moments, Features obtained by Color Co-occurrence Method and Shape based Features) we found that the use of 4 methods together (Percentage of Leaf Area Infected (PI),Texture-Based Features, Color Moments and Shape based Features) produce excellent result..
植物病害的检测关系到粮食安全问题,是严重威胁人类生命安全的重要问题。疾病诊断系统包括一系列步骤,从图像采集开始,经过预处理、分割、特征提取,最后进行分类。特征提取在任何诊断系统中都是一个非常重要的过程我们可以将这一阶段与脊柱进行比较。众所周知,这一阶段之所以如此重要,是因为提取特征的过程极大地影响了分类的工作和准确性。正确选择正确的特征可以提高系统诊断的准确性,反之亦然。该系统采集不同作物(水稻、棉花和番茄)病害的图像,输入作物的种植图像,然后将图像大小调整为固定大小,然后通过模糊直方图均衡化(FHE)对图像进行改进,然后使用基于颜色的K-means对图像进行分割,最后比较特征提取方法(叶面积感染百分比(PI)、基于纹理的特征、颜色矩、我们发现4种方法(叶面积感染百分比(PI)、基于纹理的特征、颜色矩和基于形状的特征)一起使用可以产生很好的效果。
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引用次数: 1
Antifungal Activity of Solanum nigrum Extract Against Microsporum canis, The Causative Agent of Ring Worm Disease 龙葵提取物对环虫病病原犬小孢子虫的抑菌活性研究
Pub Date : 2018-04-23 DOI: 10.30526/2017.ihsciconf.1778
Yasser Muafaq Al-qertani
The present study was performed to evaluate the anti-fungal effect of alcoholic extract of Solanum nigrum (AESn) on the growth of Microsporum canis, the causes agent of ring worm. The results of this work referred to the inhibitory effect of the studied extract on the growth of tested fungi. The percentages of inhibition were (7.88 %, 19.88%, 23.41%, 57.65%), in comparison to the control, when (2%, 4%, 6%,8% ) of tested extract were used, respectively. The data illustrated that the higher concentrations of the extract are applied, the more inhibition of fungal growth is produced.
研究了茄酒提取物(AESn)对环虫病原犬小孢子菌(Microsporum canis)生长的抑制作用。本工作的结果涉及到所研究的提取物对被试真菌生长的抑制作用。分别为2%、4%、6%、8%时,与对照相比,抑制率分别为7.88%、19.88%、23.41%、57.65%。数据表明,提取物浓度越高,对真菌生长的抑制作用越强。
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引用次数: 1
Performance of Strelitzia reginae Flowers Dye as Sensitizer for New Dye-Sensitized Solar Cell Fabrication streirenia reginae花染料在新型染料敏化太阳能电池中的性能研究
Pub Date : 2018-04-23 DOI: 10.30526/2017.IHSCICONF.1766
M. A. Al-Alwani
In current study, the dye from flowers petals of Strelitzia reginae used for the first time to prepare natural photosensitizer for DSSC fabrication. Among five different solvents used to extract the natural dye from S. reginae flowers, the ethanol extract of anthocyanin dye revealed higher absorption spectrum of 0.757a.u. at wavelength of 454nm.  A major effect of temperature was studied to increase the extraction yield. The results show that the optimal temperature was 70 °C and there was a sharp decrease of dye concentration from 0.827 at temperature of 70 °C to 0.521 at temperature of 90°C. The extract solution of flowers of S. reginae showed higher concentration in acidic media, especially at pH 4 (0.902). The C, H, N elemental analyses of natural extract showed the presence of N (10.52%), C (18.97%) and H (2.229%) contents.  The presence of anthocyanin in the extract of S. reginae and their functional groups were determined using UV-Vis spectroscopy and Fourier-Transform Infrared Spectroscopy (FTIR). The optical properties of deposited TiO2 thin film were studied using scanning electron microscope (SEM) and X-Ray Diffraction (XRD). In Addition, other confirmation that dye supported surface of TiO2, inhibition of crystallinity of TiO2 was investigated by the Energy Disperse X-ray (EDX) analysis. According to experimental results, the conversion efficiency of DSSC fabricated using anthocyanin dye obtained from S. reginae flowers was 0.1%, with short-circuit current (Isc) of 0.6 mA/cm-2, open-circuit voltage (Voc) of 0.51 V and fill factor (FF) of 36.83%.
本研究首次将streelitia reginae花瓣染料用于制备DSSC的天然光敏剂。在5种提取溶剂中,乙醇提取的花青素染料具有较高的吸收光谱,为0.57a .u。波长为454nm。研究了温度对提高提取率的主要影响。结果表明,最佳温度为70℃,染料浓度从70℃时的0.827急剧下降到90℃时的0.521。在酸性培养基中,尤其是pH为4(0.902)时,山参花提取液的浓度较高。天然提取物的C、H、N元素分析表明,天然提取物中存在N(10.52%)、C(18.97%)和H(2.229%)。采用紫外可见光谱(UV-Vis)和傅里叶变换红外光谱(FTIR)测定了山参提取物中花青素及其官能团的含量。利用扫描电子显微镜(SEM)和x射线衍射仪(XRD)研究了沉积的TiO2薄膜的光学性质。此外,通过能量分散x射线(EDX)分析研究了染料负载TiO2表面对TiO2结晶度的抑制作用。实验结果表明,用荆花花青素染料制备的DSSC转换效率为0.1%,短路电流(Isc)为0.6 mA/cm-2,开路电压(Voc)为0.51 V,填充系数(FF)为36.83%。
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引用次数: 0
The effect of Larinus maculates F. Cocoon Aqueous Extract in Some Immunological Aspects of Male Albino Mice 黄松茧水提物对雄性白化小鼠免疫功能的影响
Pub Date : 2018-04-23 DOI: 10.30526/2017.IHSCICONF.1783
Zainab Thamer Alasady, Hanady S. A. Al-Shmgania, Muna Shukri Mahmod Jwad
Traditional folk medicine is applied in the treatment of diversity chronic and acute conditions all over the worlds. This study was carried out to assess the capability of aqueous beetle cocoon extract (Lorinus maculates F.) commonly known as (Tihan) in stimulating immune response. Adult mice (5 in each group) were divided into two groups: the first one orally administered with 150 mg/kg and the second group received normal saline as a control group for 14 days. Bone marrow and spleen were proceeding for mitotic index and phagocytosis estimated. The results showed a significant increase in mitotic index and phagocytosis in treaded mice in comparing with the negative control. These results concluded that Tihan has a potential role in innate immune response.
传统民间医学在世界各地被用于治疗各种慢性和急性疾病。本研究旨在评估甲虫茧水提物(Lorinus maculates F.)刺激免疫反应的能力。将成年小鼠(每组5只)分为两组,第一组以150 mg/kg的剂量口服,第二组以生理盐水作为对照组,连续14 d。测定骨髓和脾脏的有丝分裂指数和吞噬能力。结果显示,与阴性对照相比,踏足小鼠的有丝分裂指数和吞噬能力显著增加。这些结果表明,Tihan在先天免疫应答中具有潜在的作用。
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引用次数: 0
The Congenital Malformations in White Pregnant Mice Fetus Induced by Metformin Drug During 6-18 Days of Gestation 妊娠6 ~ 18天二甲双胍致白孕小鼠胎儿先天性畸形的研究
Pub Date : 2018-04-23 DOI: 10.30526/2017.IHSCICONF.1779
F. N. Toama, A. Hamid, A. Jameel
The metformin drug  is anti-hyperglycemia  and known to cross the placenta  which leads to the fetus during pregnancy .The aim of this study is to  define the drug  effects in the fetus growth . The doses  used , therapeutic dose ( 0.18 & 0.53 ) mg25g body weight  and over dose ( 1.8 & 2.85) mg 25g body weight , administrated orally  at the beginning organogenesis stage at ( 6 -18 ) day of pregnancy in the morning . A total ( 50 ) animal  were divided into five groups .The first group control not treated , 2nd group  treated with (0.18) mg , 3rd group  with  ( 0.53 ) mg , 4th group with ( 1.8 ) mg and 5th group with    ( 2.85 ) mg .  In this study noted  some congenital malformations in the fetus compared with  normal control group , which included in the 2nd  and 3rd  group ( irregular body level , exophthalmia , micromelia , elevated some  hand fingers , simple club foot , torsion tail and wrinkle skin ) while the defects was larger in the 4th  and 5th group ( trunk abnormalities like C Shape , big head ( scaphocethaly) , cranial flexure , prosencephaly, short neck , micromelia , syndactyly  some of hand fingers and others  hemimelia  , torsion tail and some aquiline , sharply club foot  and spina bifida ) . It is  mentioning  that the malformations was less in the 2nd group  treated with (0.18) mg of  the drug  . The treatment with metformin drug during pregnancy induced the congenital malformations .
二甲双胍是一种抗高血糖药物,已知在怀孕期间会穿过胎盘导致胎儿。本研究的目的是确定药物对胎儿生长的影响。所使用的剂量,治疗剂量(0.18和0.53)mg25g体重和过量剂量(1.8和2.85)mg25g体重,在怀孕(6 -18)天的早晨开始器官发生阶段口服。将50只动物分为5组,第一组不给药,第二组给药(0.18)mg,第三组给药(0.53)mg,第四组给药(1.8)mg,第五组给药(2.85)mg。在这项研究中指出的一些先天畸形胎儿与正常对照组相比,其中包括在第二和第三组(不规则的身体水平,突眼,短肢,高架有些手手,简单的畸形足,扭转尾和皱纹的皮肤),缺陷在第四和第五大集团(C形状等异常树干,大头(scaphocethaly),颅挠曲,prosencephaly,脖子短,短肢,并指者有的手指头偏斜,有的扭尾和鹰钩,有尖锐内翻足和脊柱裂)。值得一提的是,使用0.18 mg药物治疗的第二组畸形较少。妊娠期应用二甲双胍治疗导致先天性畸形。
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引用次数: 0
Influence of Foliar Application with Abscisic Acid (ABA) and Vitamin C on Some Plant Hormones for Peas Plant (Pisum sativum L.) 叶面施ABA和维生素C对豌豆某些植物激素的影响
Pub Date : 2018-04-23 DOI: 10.30526/2017.ihsciconf.1772
W. A. AL-kaisy, Sahar F. Mahadi
A field experiment was conducted at botanical garden of Department of Biology, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, during the growth winter season of 2016-2017 to study the effect of different concentrations (0, 10, 20) mg.L-1 of abscisic acid and (0, 50, 100, 150) mg.L-1 of vitamin C and their interaction on some plant hormones of pea plant (Pisum sativum L.). The results showed that ABA 20 mg.L-1 decreased IAA about 27.44%, GA3 about 19.73% and Kinetin 15.37% while vitamin C with 150 mg.L-1 increased IAA 27.43%, GA3 45.31% and Kinetin 58.53%, but ABA increased about 23.01% for ABA and 34.93% for vitamin C compared with control plants. The interaction between them is significant for four plant hormones.
在2016-2017年冬季生长季,在巴格达大学纯科学教育学院(Ibn Al-Haitham)生物系植物园进行了田间试验,研究了不同浓度(0、10、20)mg对青松的影响。L-1的脱落酸和(0,50,100,150)mg。豌豆(Pisum sativum L.)中维生素C的L-1含量及其对某些植物激素的相互作用结果表明,ABA 20 mg。150 mg维生素C可使IAA降低27.44%,GA3降低19.73%,Kinetin降低15.37%。L-1处理使IAA、GA3和Kinetin分别增加27.43%、45.31%和58.53%,而ABA和维生素C分别增加23.01%和34.93%。这四种植物激素之间的相互作用是显著的。
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引用次数: 1
First Record of Trichodina magna Van As and Basson, 1989 (Ciliophora: Trichodinidae) from Gills of Blue Tilapia Oreochromis aureus (Steindachner, 1864) in Iraq 伊拉克蓝罗非鱼Oreochromis aureus (Steindachner, 1864)鳃中首次记录大毛癣菌Van As and Basson, 1989(毛癣菌目:毛癣菌科)
Pub Date : 2018-04-23 DOI: 10.30526/2017.ihsciconf.1771
K. Abdul-Ameer, F. K. Atwan
The Ciliophoran Trichodina magna Van As and Basson, 1989 is recorded for the first time in in Iraq from gills of the blue tilapia Oreochromis aureus (Steindachner, 1864) Iraq from Al-Graiat location on the Tigris River at Baghdad city. The description and measurements of this external parasite as well as its illustrations are given.
Ciliophoran Trichodina magna Van As and Basson, 1989年首次在伊拉克巴格达底格里斯河Al-Graiat地区的蓝色罗非鱼Oreochromis aureus (Steindachner, 1864)的鳃中记录到。给出了这种外源寄生虫的描述和测量方法,并给出了它的图解。
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引用次数: 0
Investigate of the Ability of Cronobacter sakazakii Isolated from Clinical Samples of Children Under Two Years to Induce Swimming, Swarming and Biofilm 两岁以下儿童临床分离阪崎克罗诺杆菌诱导游泳、群集和生物膜能力的研究
Pub Date : 2018-04-23 DOI: 10.30526/2017.IHSCICONF.1780
L. H. Zwein, Tharieyt abdul Rahman motlag, M. Mousa
The study included 200 samples were collected   from   children  under two   years included (50 samples from each of Cerebrospinal fluid, Blood, Stool and Urine) from, Central Children Hospital and Children's Protections Educational Hospital. Isolates bacterial were obtained cultural, microscopic and biochemical examination and diagnosed to the species by using vitek2 system. The results showed there were contamination in 6.5% of clinical samples. The diagnosed colonies which gave pink color on the MacConkey agar , golden yellow color on the Trypton Soy agar and green color on the Birillent Enterobacter sakazakii agar and gave  a probability of 99% in the vitek 2 and were identified as Cronobacter sakazakii. The identification revealed of thirteen isolates: 6(46.16%) isolated from Cerebrospinal fluid samples, 7(53.84%) isolated from blood samples and not isolated bacteria from stool and urine samples. The results of the investigation of some virulence factors showed that all bacteria isolates were able to swimming with a diameter ranging (1-9 mm) and swarming with a diameter ranging (1-40 mm) and their  ability to biofilm formation  by using three methods. The results show the ability  of  isolates to form biofilm by using  Congo red media  methods where it is 12 (92.30 %) out of 13 isolated bacteria belonging to C. sakazakii  able to form biofilm on the Congo red media  which is 3 (23.07%) were  strong production  biofilm ,   8 (61.53%)  were intermediate  production  biofilm and  1 (7.69% ) were weak  biofilm formation , while the 1 (7.69%)  unable to form biofilm.  Tubes method were all isolates were able to form biofilm, it were found that 3 (23.07%)  isolates strong, and 8 (61.53%) intermediate  and 2( 15.38%)  weak biofilm formation. Microtiter plate method  gave 5 (38.46 %) isolates strong, 6 (46.15%) intermediate and 1 (7.69%) weak biofilm formation.
本研究收集了200例两岁以下儿童样本(脑脊液、血液、粪便和尿液各50例),分别来自中心儿童医院和儿童保护教育医院。对分离菌进行培养、镜检和生化检查,并用vitek2系统进行菌种诊断。结果显示,6.5%的临床样品存在污染。诊断菌落在MacConkey琼脂上呈粉红色,在Trypton Soy琼脂上呈金黄色,在Birillent阪崎肠杆菌琼脂上呈绿色,在第2周的概率为99%,鉴定为阪崎肠杆菌。其中,脑脊液分离到6株(46.16%),血液分离到7株(53.84%),粪便和尿液中未分离到细菌。对部分毒力因子的调查结果表明,三种方法分离的细菌均能在直径1 ~ 9 mm的范围内游动,在直径1 ~ 40 mm的范围内成群,并能形成生物膜。结果表明,在刚果红培养基上分离的13株阪崎梭菌中,有12株(92.30%)在刚果红培养基上形成生物膜,其中3株(23.07%)为强生成生物膜,8株(61.53%)为中生成生物膜,1株(7.69%)为弱生成生物膜,1株(7.69%)不能形成生物膜。试管法检测所有分离株均能形成生物膜,结果发现3株(23.07%)形成强生物膜,8株(61.53%)形成中等生物膜,2株(15.38%)形成弱生物膜。微滴板法分离出5株(38.46%)强、6株(46.15%)中、1株(7.69%)弱生物膜。
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引用次数: 0
期刊
Ibn Al-Haitham Journal For Pure And Applied Science
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