Pub Date : 2023-07-31DOI: 10.56042/ijeb.v61i08.3879
Suzan Murato ğ lu Severcan, Gülce Koca, C. Severcan, Canan Yılmaz, ÖzgeTu ğ çe Pa ş ao ğ lu, Hatice Pa ş ao ğ lu
Elucidation of the positive effects of curcumin on oxidative damage and inflammation, as well as clarifying the parameters related to adipose tissue and liver, would contribute furthermore to literature. Here, we have demonstrated the potential healing effects of curcumin in rats with metabolic syndrome (MetS). Twenty-four adult male Wistar albino rats were selected for the study. Only corn oil was administered to the control group rats, while corn oil and 20% fructose were administered to the MetS group rats for eight weeks. The curcumin dose groups were administered 100 and 200 mg/kg of curcumin dissolved in corn oil along with 20% fructose. Plasma TBARS, IL-6, adipose AFABP-4 and liver FGF-21 levels were determined using the ELISA method. It was observed that plasma TBARS and IL-6 levels were significantly decreased in the 100 and 200 mg/kg curcumin dose groups compared to the MetS group ( P ≤ 0.008). Adipose tissue A-FABP-4 levels in the 200 mg/kg curcumin dose group were significantly decreased compared to the MetS group ( P ≤ 0.008). FGF-21 levels in the 100 mg/kg and 200 mg/kg curcumin dose groups were significantly increased compared to the MetS group ( P ≤ 0.008). The healing effects of curcumin administration on MetS were effective on inflammation and lipid peroxidation. Curcumin administration decreased the adipose tissue AFABP-4 levels and increased the liver FGF-21 levels.
{"title":"Effect of curcumin on plasma TBARS and IL-6, adipose tissue AFABP-4 and liver FGF-21 in fructose-induced metabolic syndrome rats","authors":"Suzan Murato ğ lu Severcan, Gülce Koca, C. Severcan, Canan Yılmaz, ÖzgeTu ğ çe Pa ş ao ğ lu, Hatice Pa ş ao ğ lu","doi":"10.56042/ijeb.v61i08.3879","DOIUrl":"https://doi.org/10.56042/ijeb.v61i08.3879","url":null,"abstract":"Elucidation of the positive effects of curcumin on oxidative damage and inflammation, as well as clarifying the parameters related to adipose tissue and liver, would contribute furthermore to literature. Here, we have demonstrated the potential healing effects of curcumin in rats with metabolic syndrome (MetS). Twenty-four adult male Wistar albino rats were selected for the study. Only corn oil was administered to the control group rats, while corn oil and 20% fructose were administered to the MetS group rats for eight weeks. The curcumin dose groups were administered 100 and 200 mg/kg of curcumin dissolved in corn oil along with 20% fructose. Plasma TBARS, IL-6, adipose AFABP-4 and liver FGF-21 levels were determined using the ELISA method. It was observed that plasma TBARS and IL-6 levels were significantly decreased in the 100 and 200 mg/kg curcumin dose groups compared to the MetS group ( P ≤ 0.008). Adipose tissue A-FABP-4 levels in the 200 mg/kg curcumin dose group were significantly decreased compared to the MetS group ( P ≤ 0.008). FGF-21 levels in the 100 mg/kg and 200 mg/kg curcumin dose groups were significantly increased compared to the MetS group ( P ≤ 0.008). The healing effects of curcumin administration on MetS were effective on inflammation and lipid peroxidation. Curcumin administration decreased the adipose tissue AFABP-4 levels and increased the liver FGF-21 levels.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49190355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-31DOI: 10.56042/ijeb.v61i08.3674
L. Yang, Ya., Nan Liu, Yi Gu, Lan Zhu, Q. Guo
In a clinical setting, the likelihood of doxorubicin (DOX) causing cardiotoxicity is high. However, the underlying mechanism remains obscure. In this study, we investigated whether DOX toxicity is associated with the deregulation of circular RNA_0004212 (circ_0004214). Circ_0004214, microRNA-22-3p (miR-22-3p), and GATA binding protein 4 (GATA4) expression in human cardiomyocyte AC16 cells was detected via RT-qPCR. Lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) production, malondialdehyde (MDA) content, and 4-hydroxynonenal (4-HNE) content were assessed using corresponding commercial kits. Cell viability and apoptosis were analyzed using cell counting kit-8 (CCK-8) and flow cytometry assays. Western blot assay was used to evaluate apoptosis-related markers and GATA4 protein levels. Dual-luciferase reporter validated the relationship between miR-22-3p and circ_0004214 or GATA4. Declined circ_0004214 was viewed in DOX-treated AC16 cells. DOX treatment weakened cell viability, and promoted oxidative stress and apoptosis, which was ameliorated via circ_0004214 overexpression. In addition, circ_0004214 promoted GATA4 expression by decoying miR-22-3p. Overall, the results have demonstrated that circ_0004214 protects against DOX-induced cardiotoxicity via governing miR-22-3p/GATA4 pathway, and thereby reveal promising therapeutic strategies against cardiotoxicity.
{"title":"Circ_0004214 prevents human cardiomyocytes from doxorubicin induced cardiotoxicity by governing the miR-22-3p/GATA4 pathway","authors":"L. Yang, Ya., Nan Liu, Yi Gu, Lan Zhu, Q. Guo","doi":"10.56042/ijeb.v61i08.3674","DOIUrl":"https://doi.org/10.56042/ijeb.v61i08.3674","url":null,"abstract":"In a clinical setting, the likelihood of doxorubicin (DOX) causing cardiotoxicity is high. However, the underlying mechanism remains obscure. In this study, we investigated whether DOX toxicity is associated with the deregulation of circular RNA_0004212 (circ_0004214). Circ_0004214, microRNA-22-3p (miR-22-3p), and GATA binding protein 4 (GATA4) expression in human cardiomyocyte AC16 cells was detected via RT-qPCR. Lactate dehydrogenase (LDH) release, reactive oxygen species (ROS) production, malondialdehyde (MDA) content, and 4-hydroxynonenal (4-HNE) content were assessed using corresponding commercial kits. Cell viability and apoptosis were analyzed using cell counting kit-8 (CCK-8) and flow cytometry assays. Western blot assay was used to evaluate apoptosis-related markers and GATA4 protein levels. Dual-luciferase reporter validated the relationship between miR-22-3p and circ_0004214 or GATA4. Declined circ_0004214 was viewed in DOX-treated AC16 cells. DOX treatment weakened cell viability, and promoted oxidative stress and apoptosis, which was ameliorated via circ_0004214 overexpression. In addition, circ_0004214 promoted GATA4 expression by decoying miR-22-3p. Overall, the results have demonstrated that circ_0004214 protects against DOX-induced cardiotoxicity via governing miR-22-3p/GATA4 pathway, and thereby reveal promising therapeutic strategies against cardiotoxicity.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48056937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-28DOI: 10.56042/ijeb.v61i07.2536
{"title":"BRMS1L mediated effect of miR-20a-5p expression on proliferation and apoptosis of gastric cancer cells","authors":"","doi":"10.56042/ijeb.v61i07.2536","DOIUrl":"https://doi.org/10.56042/ijeb.v61i07.2536","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45927334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-28DOI: 10.56042/ijeb.v61i07.2468
{"title":"Effects of modified Bushen Huoxue Prescription combined with active immunotherapy on immunomodulatory functions and pregnancy outcomes of patients with unexplained recurrent spontaneous abortion","authors":"","doi":"10.56042/ijeb.v61i07.2468","DOIUrl":"https://doi.org/10.56042/ijeb.v61i07.2468","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48509077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-28DOI: 10.56042/ijeb.v61i07.2214
{"title":"Serum triacylglycerol: A putative early biomarker of disease severity of Type 2 diabetes mellitus compared to microalbuminuria","authors":"","doi":"10.56042/ijeb.v61i07.2214","DOIUrl":"https://doi.org/10.56042/ijeb.v61i07.2214","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49364938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-28DOI: 10.56042/ijeb.v61i07.2216
{"title":"Evaluation of radioprotective properties of Trianthema portulacastrum L. stem extract in vivo","authors":"","doi":"10.56042/ijeb.v61i07.2216","DOIUrl":"https://doi.org/10.56042/ijeb.v61i07.2216","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42942837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-28DOI: 10.56042/ijeb.v61i07.2464
{"title":"Effect of external washing with self-made Xiaofeng Jiedu powder on expressions of serum inflammatory cytokines in patients with cutaneous adverse events induced by programmed cell death protein 1 inhibitor","authors":"","doi":"10.56042/ijeb.v61i07.2464","DOIUrl":"https://doi.org/10.56042/ijeb.v61i07.2464","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47492717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-28DOI: 10.56042/ijeb.v61i07.2284
{"title":"From error to uncertainty: A search for variabilities in the laboratory reports","authors":"","doi":"10.56042/ijeb.v61i07.2284","DOIUrl":"https://doi.org/10.56042/ijeb.v61i07.2284","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43164756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-28DOI: 10.56042/ijeb.v61i07.2467
{"title":"Diagnostic and prognostic values of abdominal ultrasound combined with urinary intestinal fatty acid binding protein for neonatal necrotizing enterocolitis","authors":"","doi":"10.56042/ijeb.v61i07.2467","DOIUrl":"https://doi.org/10.56042/ijeb.v61i07.2467","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43704857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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