Pub Date : 2023-12-26DOI: 10.56042/ijeb.v62i01.1420
{"title":"Allium cepa L., a potential hepatoprotective and antituberculosis agent","authors":"","doi":"10.56042/ijeb.v62i01.1420","DOIUrl":"https://doi.org/10.56042/ijeb.v62i01.1420","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139155930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-24DOI: 10.56042/ijeb.v61i12.4003
Özlem Özge Y ı lmaz, Tolga Polat, B. Eken, Gökhan Tuna, B. Aslan, K. Ulucan
Nuclear respiration factor 2 is an important respiratory factor for cellular metabolism formed by polypeptides produced by the GA binding protein transcription factor subunit alpha ( GABPA ) and GA binding protein transcription factor subunit beta 1 ( GAPPB1 ) genes. A functional polymorphism on the GABPB1 gene (rs7181866, G/A) alters the cellular respiration metabolism by affecting the protein dynamics. In this context, here, we examined the distribution of GABPB1 rs718186 polymorphism in professional rugby players to see its relationship with their stamina. Twelve rugby players participated in the study. Genotyping was performed by Real-Time PCR after DNAs were isolated from blood cells using commercially available isolation kits. In our cohort, the AA genotype and the A allele were superior to the AG and GG genotypes and the G allele. There was no statistically significant difference in terms of Genotypes (p=0.9027) and allelic groups (p=0.6948) in the athlete and control groups. Studies have revealed the relationship between endurance characteristics of athletes and rs7181866 AA genotype and A allele. These studies will be an important data source for future studies in sports genetics and GAPPB1 analysis.
核呼吸因子 2 是由 GA 结合蛋白转录因子亚基 alpha（GABPA）和 GA 结合蛋白转录因子亚基 beta 1（GAPPB1）基因产生的多肽形成的一种重要的细胞呼吸代谢因子。GABPB1 基因上的一个功能性多态性（rs7181866，G/A）会影响蛋白质的动态变化，从而改变细胞的呼吸代谢。在此背景下，我们研究了 GABPB1 rs718186 多态性在职业橄榄球运动员中的分布情况，以了解其与运动员耐力的关系。12 名橄榄球运动员参与了这项研究。使用市售分离试剂盒从血细胞中分离出 DNA 后，通过 Real-Time PCR 进行基因分型。在我们的队列中，AA 基因型和 A 等位基因优于 AG 和 GG 基因型和 G 等位基因。运动员组和对照组在基因型（P=0.9027）和等位基因组（P=0.6948）方面没有明显的统计学差异。研究揭示了运动员耐力特征与 rs7181866 AA 基因型和 A 等位基因之间的关系。这些研究将成为未来运动遗传学研究和 GAPPB1 分析的重要数据来源。
{"title":"Distribution of GABPB1 gene rs7181866 polymorphism in rugby players","authors":"Özlem Özge Y ı lmaz, Tolga Polat, B. Eken, Gökhan Tuna, B. Aslan, K. Ulucan","doi":"10.56042/ijeb.v61i12.4003","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.4003","url":null,"abstract":"Nuclear respiration factor 2 is an important respiratory factor for cellular metabolism formed by polypeptides produced by the GA binding protein transcription factor subunit alpha ( GABPA ) and GA binding protein transcription factor subunit beta 1 ( GAPPB1 ) genes. A functional polymorphism on the GABPB1 gene (rs7181866, G/A) alters the cellular respiration metabolism by affecting the protein dynamics. In this context, here, we examined the distribution of GABPB1 rs718186 polymorphism in professional rugby players to see its relationship with their stamina. Twelve rugby players participated in the study. Genotyping was performed by Real-Time PCR after DNAs were isolated from blood cells using commercially available isolation kits. In our cohort, the AA genotype and the A allele were superior to the AG and GG genotypes and the G allele. There was no statistically significant difference in terms of Genotypes (p=0.9027) and allelic groups (p=0.6948) in the athlete and control groups. Studies have revealed the relationship between endurance characteristics of athletes and rs7181866 AA genotype and A allele. These studies will be an important data source for future studies in sports genetics and GAPPB1 analysis.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139239444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-24DOI: 10.56042/ijeb.v61i12.6813
{"title":"Correlation between cervical thickness and histological severity of cervical intraepithelial neoplasia: A transvaginal ultrasound investigation","authors":"","doi":"10.56042/ijeb.v61i12.6813","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.6813","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139242387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-24DOI: 10.56042/ijeb.v61i12.2999
Kenan Çevik, Mustafa Ertan Ay, Özlem İzci, Anil Ay, Didem Derici Tombak, Yildirim Tuba, Kabasakal, Mehmet Emin, Erdal
The microRNA (miR)-29 family has been deregulated in several types of hematologic malignancies. However, role of this family and their target genes DNMT3A (DNA methyltransferase 3A) and TET2 (Ten-Eleven Translocation 2) remains unclear. Here, we have made an attempt to determine the relative expression levels of three miRNAs and target genes in patients with newly diagnosed Multiple myeloma (MM) using quantitative real-time PCR. Moreover, the expression levels of selected miRNAs and genes and their correlations with clinical parameters were compared and analyzed. The ROC curve was used to analyze their diagnostic efficacy for MM. The expression level of hsa-miR-29b-3p was significantly higher in patients with newly diagnosed MM compared with the control group. ROC analysis showed that hsa-miR-29b-3p demonstrated a moderate diagnostic power in MM. The relative expression level of hsa-miR-29b-3p in patients with high LDH levels was markedly reduced compared to that in patients with normal and low LDH levels . DNMT3A expression level was significantly increased in patients with high LDH levels and patients with lambda light chain. Our results indicate that hsa-miR-29b-3p may be used as a potential biomarker in the diagnosis of MM.
{"title":"Expression levels and clinical significances of hsa-miR-29 family and their target genes in the bone marrow of patients with multiple myeloma","authors":"Kenan Çevik, Mustafa Ertan Ay, Özlem İzci, Anil Ay, Didem Derici Tombak, Yildirim Tuba, Kabasakal, Mehmet Emin, Erdal","doi":"10.56042/ijeb.v61i12.2999","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.2999","url":null,"abstract":"The microRNA (miR)-29 family has been deregulated in several types of hematologic malignancies. However, role of this family and their target genes DNMT3A (DNA methyltransferase 3A) and TET2 (Ten-Eleven Translocation 2) remains unclear. Here, we have made an attempt to determine the relative expression levels of three miRNAs and target genes in patients with newly diagnosed Multiple myeloma (MM) using quantitative real-time PCR. Moreover, the expression levels of selected miRNAs and genes and their correlations with clinical parameters were compared and analyzed. The ROC curve was used to analyze their diagnostic efficacy for MM. The expression level of hsa-miR-29b-3p was significantly higher in patients with newly diagnosed MM compared with the control group. ROC analysis showed that hsa-miR-29b-3p demonstrated a moderate diagnostic power in MM. The relative expression level of hsa-miR-29b-3p in patients with high LDH levels was markedly reduced compared to that in patients with normal and low LDH levels . DNMT3A expression level was significantly increased in patients with high LDH levels and patients with lambda light chain. Our results indicate that hsa-miR-29b-3p may be used as a potential biomarker in the diagnosis of MM.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-24DOI: 10.56042/ijeb.v61i12.1532
Semih Öz, Fatma Y ı ld ı z, Ş. A. Kabay, Hakan Ş entürk, Dilek Buruko ğ lu Dönmez, Cansu Özbayer, Mehmet Cengiz Üstüner, Bilgin Kayg ı s ı z, K. Civi, Çetin, Hilmi Özden
Acute kidney injury (AKI), an immediate loss of renal function, leads to high mortality, and ischemia-reperfusion (I/R) injury is considered as one of the main causes of AKI. Inflammation and oxidative stress are known to play an important role in AKI. On the other hand, the earthworm extract, used in traditional medicine, is known to possess various biological and pharmacological activities viz. antiapoptotic, anticoagulative, fibrinolytic, anti-inflammatory, antioxidative stress, peripheral nerve regeneration, bone regeneration and wound healing. Hence, in this study, we investigated the protective effect of the earthworm Lumbricus rubellus Hoffmeister extract (LE) after nephrectomy, against oxidative stress occurring during renal ischemia/reperfusion (I/R) injury. A total of 10-12 weeks old Sprague Dawley male rats were divided into five groups (n=8). Group I (control), Group II (I/R), Group III (I/R + 10 mg/kg LE), Group IV (I/R + 20 mg/kg LE) and Group V (I/R + 40 mg/kg LE). All rats except in Gr. I were applied ischemia for 45 min and reperfusion for 24 h. At the end of the experiment, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) enzyme activities and malondialdehyde (MDA) levels were evaluated. In addition, kidney tissues were evaluated histologically. In results, the MDA and GPx level of the I/R group were found to be significantly higher than the control and LE groups. SOD activity of the control group did not differ when compared to LE groups and CAT levels were not significantly different between all groups. In addition, in Gr. III-V we observed nearly normal renal cortex and renal tubules. The present study, thus demonstrates that the extract of L. rubellus prevents renal I/R injury and induced biochemical and histological changes in the renal tissues in rats.
{"title":"Protective effect of Lumbricus rubellus Hoffmeister extract in experimental renal ischemia/reperfusion injury in the nephrectomy rats","authors":"Semih Öz, Fatma Y ı ld ı z, Ş. A. Kabay, Hakan Ş entürk, Dilek Buruko ğ lu Dönmez, Cansu Özbayer, Mehmet Cengiz Üstüner, Bilgin Kayg ı s ı z, K. Civi, Çetin, Hilmi Özden","doi":"10.56042/ijeb.v61i12.1532","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.1532","url":null,"abstract":"Acute kidney injury (AKI), an immediate loss of renal function, leads to high mortality, and ischemia-reperfusion (I/R) injury is considered as one of the main causes of AKI. Inflammation and oxidative stress are known to play an important role in AKI. On the other hand, the earthworm extract, used in traditional medicine, is known to possess various biological and pharmacological activities viz. antiapoptotic, anticoagulative, fibrinolytic, anti-inflammatory, antioxidative stress, peripheral nerve regeneration, bone regeneration and wound healing. Hence, in this study, we investigated the protective effect of the earthworm Lumbricus rubellus Hoffmeister extract (LE) after nephrectomy, against oxidative stress occurring during renal ischemia/reperfusion (I/R) injury. A total of 10-12 weeks old Sprague Dawley male rats were divided into five groups (n=8). Group I (control), Group II (I/R), Group III (I/R + 10 mg/kg LE), Group IV (I/R + 20 mg/kg LE) and Group V (I/R + 40 mg/kg LE). All rats except in Gr. I were applied ischemia for 45 min and reperfusion for 24 h. At the end of the experiment, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) enzyme activities and malondialdehyde (MDA) levels were evaluated. In addition, kidney tissues were evaluated histologically. In results, the MDA and GPx level of the I/R group were found to be significantly higher than the control and LE groups. SOD activity of the control group did not differ when compared to LE groups and CAT levels were not significantly different between all groups. In addition, in Gr. III-V we observed nearly normal renal cortex and renal tubules. The present study, thus demonstrates that the extract of L. rubellus prevents renal I/R injury and induced biochemical and histological changes in the renal tissues in rats.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-24DOI: 10.56042/ijeb.v61i12.2738
Özer Aylin, Gürpinar, Emre Kubat
Dabigatran (DBG) is an oral direct thrombin inhibitor used for prevention of systemic embolism and venous thromboembolism. The major side effect of DBG is gastrointestinal upset. In the present study, we have investigated whether N-acetyl cysteine (NAC) showed a protective effect on dabigatran-induced cytotoxicity in the in vitro setting. The medium not containing DBG but containing NAC were served to assay the effect of NAC on cell proliferation and apoptosis. Comparing DAB and all other groups, the cell viability was the lowest in the D group. However, there was no statistically significant difference between the NAC I and DBG-NAC I group, while the difference was statistically significant compared to all other groups. The cells in the DBG group showed a degenerative and round-shaped morphology with nuclear condensation. In other dilutions, the cell morphology was healthy with a fibroblastic morphology. Based on our study results, NAC at high concentrations exerts cytoprotective effects against DBG, while moderate or low concentrations have no favorable effect on cell viability of NAC. Although using concomitant NAC at appropriate doses appears to be effective agent against dabigatran cytotoxicity in the current study, further experimental and clinical studies are needed to confirm our findings.
达比加群（Dabigatran，DBG）是一种口服直接凝血酶抑制剂，用于预防全身性栓塞和静脉血栓栓塞。达比加群的主要副作用是胃肠道不适。在本研究中，我们研究了 N-乙酰半胱氨酸（NAC）在体外环境中是否对达比加群诱导的细胞毒性有保护作用。在不含达比加群但含有 NAC 的培养基中检测 NAC 对细胞增殖和凋亡的影响。比较 DAB 和所有其他组，D 组的细胞存活率最低。然而，NAC I 组和 DBG-NAC I 组之间的差异无统计学意义，而与其他各组相比，差异有统计学意义。DBG 组的细胞形态变性，呈圆形，核凝缩。在其他稀释液中，细胞形态为健康的成纤维细胞形态。根据我们的研究结果，高浓度的 NAC 对 DBG 具有细胞保护作用，而中等或低浓度的 NAC 对细胞存活率没有有利影响。虽然在目前的研究中，同时使用适当剂量的 NAC 似乎能有效抑制达比加群的细胞毒性，但还需要进一步的实验和临床研究来证实我们的发现。
{"title":"Prevention of dabigatran ınduced cytotoxicity by N-acetyl cysteine: An ın vitro study","authors":"Özer Aylin, Gürpinar, Emre Kubat","doi":"10.56042/ijeb.v61i12.2738","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.2738","url":null,"abstract":"Dabigatran (DBG) is an oral direct thrombin inhibitor used for prevention of systemic embolism and venous thromboembolism. The major side effect of DBG is gastrointestinal upset. In the present study, we have investigated whether N-acetyl cysteine (NAC) showed a protective effect on dabigatran-induced cytotoxicity in the in vitro setting. The medium not containing DBG but containing NAC were served to assay the effect of NAC on cell proliferation and apoptosis. Comparing DAB and all other groups, the cell viability was the lowest in the D group. However, there was no statistically significant difference between the NAC I and DBG-NAC I group, while the difference was statistically significant compared to all other groups. The cells in the DBG group showed a degenerative and round-shaped morphology with nuclear condensation. In other dilutions, the cell morphology was healthy with a fibroblastic morphology. Based on our study results, NAC at high concentrations exerts cytoprotective effects against DBG, while moderate or low concentrations have no favorable effect on cell viability of NAC. Although using concomitant NAC at appropriate doses appears to be effective agent against dabigatran cytotoxicity in the current study, further experimental and clinical studies are needed to confirm our findings.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-24DOI: 10.56042/ijeb.v61i12.6811
Yumei Li, Qing Zhang, Haixia Zhu, De-ta Chen, Tian-you Fan
Acute ankle sprain is one of the most common lower limb injuries particularly in athletes and it accounts for 16-40% of all sports-related injuries. A significant proportion of patients experience persistent residual symptoms and recurrence. Traditional Chinese medicine (TCM) offers effective treatment in terms of pain relief, swelling reduction, functional improvement, and shorter disease duration. This study provides a comprehensive analysis of the active ingredients, molecular targets, signaling pathways, and Longji Xiaozhong ointment's clinical efficacy for treating acute ankle sprains. We followed network pharmacology approach to identify the molecular targets and mechanism of action of Longji Xiaozhong ointment in treating acute ankle sprain and to validate its clinical value using relevant clinical data. Initially, Longji Xiaozhong Ointment's active ingredients were screened using the TCMSP platform based on drug properties similar to DLP ≥0.18 and oral bioavailability OB ≥30%. The molecular targets were identified from the ETCM database, followed by constructing protein-protein interaction (PPI) network diagrams and GO and pathway enrichment analysis on key genes. Furthermore, the UniProt database was used to investigate the 3D structures of these key genes. Finally, the clinical data of 111 patients with acute ankle sprains were retrospectively analyzed and further explored by comparing the different curative effects of Longji Xiaozhong ointment with Celebrex and Shexiang Analgesic ointment to check the clinical value of Longji Xiaozhong ointment in treating acute ankle sprain. In the TCMSP database, we found that there were 42 active ingredients in Longji Xiaozhong ointment, including 13 kinds of Strychnosnux-vomica L., 7 kinds of Ligusticum chuanxiong , and 22 kinds of Safflowers ( Carthamus tinctorius ), and a total of 3206 target genes were obtained. After applying the OB ≥50% criteriaand DL ≥0.5, 19 key target genes were selected based on their correspondence to the active ingredients. Protein mutual aid network construction and module analysis yielded two high-scoring Clusters and identified seven key proteins, including SLC6A4, ADRB2, ADRA1B, CHRM1, F2, OPRM1, and OPRD1. Functional enrichment analysis (FEA) of candidate target genes of Strychnosnux-vomica , Ligusticum chuanxiong and Safflowers in Longji Xiaozhong ointment in ETCM database showed SUMOylation of intracellular receptors, blockade of NMDA receptors, and activation of GABA A receptors, lipid metabolism regulationby peroxisome proliferator-activated receptor alpha (PPARα). PPARA activates aspects of gene expression and transcriptional activation of mitochondrial biogenesis. On retrospective analysis, all patients were divided into Group A: Celebrex combined with brace immobilization group (36 cases); Group B: Shexiang Jietong ointment combined with brace immobilization group (37 cases); and Group C: Longji Xiaozhong ointment combined with brace immobilization group (38
急性踝关节扭伤是最常见的下肢损伤之一，尤其是在运动员中，占所有运动相关损伤的 16-40%。相当一部分患者会出现持续的后遗症和复发。传统中医在止痛、消肿、改善功能、缩短病程等方面提供了有效的治疗方法。本研究全面分析了龙胆泻肝膏治疗急性踝关节扭伤的有效成分、分子靶点、信号通路和临床疗效。我们采用网络药理学方法，确定了龙胆泻肝膏治疗急性踝关节扭伤的分子靶点和作用机制，并利用相关临床数据验证了其临床价值。首先，基于DLP≥0.18和口服生物利用度OB≥30%的药物性质，利用TCMSP平台筛选龙胆泻肝膏的有效成分。从 ETCM 数据库中确定了分子靶点，然后构建了蛋白质-蛋白质相互作用（PPI）网络图，并对关键基因进行了 GO 和通路富集分析。此外，还利用 UniProt 数据库研究了这些关键基因的三维结构。最后，回顾性分析了111例急性踝关节扭伤患者的临床资料，并通过比较龙胆泻肝膏与西乐葆和十香止痛膏的不同疗效，进一步探讨了龙胆泻肝膏治疗急性踝关节扭伤的临床价值。在中药数据库（TCMSP）中，我们发现龙胆泻肝膏有 42 种有效成分，包括 13 种马钱子、7 种川芎、22 种红花，共获得 3206 个靶基因。以OB≥50%和DL≥0.5为标准，根据目标基因与有效成分的对应关系筛选出19个关键目标基因。蛋白质互助网络构建和模块分析产生了两个高分簇，并确定了 7 个关键蛋白质，包括 SLC6A4、ADRB2、ADRA1B、CHRM1、F2、OPRM1 和 OPRD1。ETCM 数据库对川芎、红花龙胆泻肝膏候选靶基因的功能富集分析（FEA）显示，细胞内受体的 SUMO 化、NMDA 受体的阻断、GABA A 受体的激活、过氧化物酶体增殖激活受体α（PPARα）对脂质代谢的调控。PPARA 可激活线粒体生物生成的基因表达和转录激活。经过回顾性分析，所有患者被分为 A 组：A组：西乐葆联合支具固定组（36 例）；B组：畲乡洁通软膏联合支具固定组（37 例）；C组：龙胆泻肝丸联合支具固定组（37 例）：龙胆泻肝膏联合支具固定组（38例）。治疗后，龙胆泻肝膏联合支具固定组的 VAS 评分、肿胀程度和范围评分均明显低于其他两组，在日常活动、运动、生活质量和再扭伤发生率方面的效果明显优于其他两组。我们的研究结果得到了临床数据的支持，证明龙胆泻肝膏能有效缓解肿胀和疼痛，加速踝关节韧带的修复，增强踝关节功能，改善踝关节稳定性。
{"title":"Molecular targets and mechanism of Longji Xiaozhong ointment in treating acute ankle sprain: A network pharmacology based analysis","authors":"Yumei Li, Qing Zhang, Haixia Zhu, De-ta Chen, Tian-you Fan","doi":"10.56042/ijeb.v61i12.6811","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.6811","url":null,"abstract":"Acute ankle sprain is one of the most common lower limb injuries particularly in athletes and it accounts for 16-40% of all sports-related injuries. A significant proportion of patients experience persistent residual symptoms and recurrence. Traditional Chinese medicine (TCM) offers effective treatment in terms of pain relief, swelling reduction, functional improvement, and shorter disease duration. This study provides a comprehensive analysis of the active ingredients, molecular targets, signaling pathways, and Longji Xiaozhong ointment's clinical efficacy for treating acute ankle sprains. We followed network pharmacology approach to identify the molecular targets and mechanism of action of Longji Xiaozhong ointment in treating acute ankle sprain and to validate its clinical value using relevant clinical data. Initially, Longji Xiaozhong Ointment's active ingredients were screened using the TCMSP platform based on drug properties similar to DLP ≥0.18 and oral bioavailability OB ≥30%. The molecular targets were identified from the ETCM database, followed by constructing protein-protein interaction (PPI) network diagrams and GO and pathway enrichment analysis on key genes. Furthermore, the UniProt database was used to investigate the 3D structures of these key genes. Finally, the clinical data of 111 patients with acute ankle sprains were retrospectively analyzed and further explored by comparing the different curative effects of Longji Xiaozhong ointment with Celebrex and Shexiang Analgesic ointment to check the clinical value of Longji Xiaozhong ointment in treating acute ankle sprain. In the TCMSP database, we found that there were 42 active ingredients in Longji Xiaozhong ointment, including 13 kinds of Strychnosnux-vomica L., 7 kinds of Ligusticum chuanxiong , and 22 kinds of Safflowers ( Carthamus tinctorius ), and a total of 3206 target genes were obtained. After applying the OB ≥50% criteriaand DL ≥0.5, 19 key target genes were selected based on their correspondence to the active ingredients. Protein mutual aid network construction and module analysis yielded two high-scoring Clusters and identified seven key proteins, including SLC6A4, ADRB2, ADRA1B, CHRM1, F2, OPRM1, and OPRD1. Functional enrichment analysis (FEA) of candidate target genes of Strychnosnux-vomica , Ligusticum chuanxiong and Safflowers in Longji Xiaozhong ointment in ETCM database showed SUMOylation of intracellular receptors, blockade of NMDA receptors, and activation of GABA A receptors, lipid metabolism regulationby peroxisome proliferator-activated receptor alpha (PPARα). PPARA activates aspects of gene expression and transcriptional activation of mitochondrial biogenesis. On retrospective analysis, all patients were divided into Group A: Celebrex combined with brace immobilization group (36 cases); Group B: Shexiang Jietong ointment combined with brace immobilization group (37 cases); and Group C: Longji Xiaozhong ointment combined with brace immobilization group (38 ","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-24DOI: 10.56042/ijeb.v61i12.4370
Metin Budak, Mahmut Alp K ı l ı ç, T. Kalkan, H. Tuncel
The dysregulation of beta-catenin, a key regulator of cadherin-mediated cell adhesion and crucial for embryonic development and adult tissue processes, has been implicated in various cancers, including colon cancer. Meanwhile, there have been longstanding concerns about the potential carcinogenic effects of magnetic fields. In this study, we investigated the possible relationship between beta-catenin dysfunction and 50 Hz sinusoidal magnetic fields (SMF) using an animal model of N-methyl-N-nitrosourea (MNU)-induced rat colon tumors. To assess beta-catenin phosphorylation, genomic DNA was extracted from 58 samples using a commercial extraction kit, and the target gene region corresponding to an important phosphorylation site of beta-catenin was amplified via polymerase chain reaction (PCR). The amplified samples were subsequently analyzed using the single-strand conformation polymorphism (SSCP) method to detect any differences between the experimental groups. Surprisingly, our results revealed no significant differences in beta-catenin gene phosphorylation sites among the groups. These findings suggest that 50 Hz SMF exposure may not directly impact beta-catenin dysfunction in the context of MNU-induced rat colon tumors. Implications of these results and avenues for further research are discussed.
{"title":"No mutation effect of 50 Hz sinusoidal magnetic field on beta catenin gene phosphorylation site in N-methyl-N-nitrosourea (MNU) induced colon tumor model","authors":"Metin Budak, Mahmut Alp K ı l ı ç, T. Kalkan, H. Tuncel","doi":"10.56042/ijeb.v61i12.4370","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.4370","url":null,"abstract":"The dysregulation of beta-catenin, a key regulator of cadherin-mediated cell adhesion and crucial for embryonic development and adult tissue processes, has been implicated in various cancers, including colon cancer. Meanwhile, there have been longstanding concerns about the potential carcinogenic effects of magnetic fields. In this study, we investigated the possible relationship between beta-catenin dysfunction and 50 Hz sinusoidal magnetic fields (SMF) using an animal model of N-methyl-N-nitrosourea (MNU)-induced rat colon tumors. To assess beta-catenin phosphorylation, genomic DNA was extracted from 58 samples using a commercial extraction kit, and the target gene region corresponding to an important phosphorylation site of beta-catenin was amplified via polymerase chain reaction (PCR). The amplified samples were subsequently analyzed using the single-strand conformation polymorphism (SSCP) method to detect any differences between the experimental groups. Surprisingly, our results revealed no significant differences in beta-catenin gene phosphorylation sites among the groups. These findings suggest that 50 Hz SMF exposure may not directly impact beta-catenin dysfunction in the context of MNU-induced rat colon tumors. Implications of these results and avenues for further research are discussed.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-01DOI: 10.56042/ijeb.v61i11.1764
Cyclophosphamide (CTX) is an effective chemotherapeutic agent. Gonadotropins are molecules with various actions. We investigated the effects of gonadotropins on the peripheral blood, bone marrow, and spleen in rats given CTX. Three groups were formed: Control (C) group with no process; Sham (S) group: Physiological saline was applied; CTX group: A single dose of 200 mg/kg and 8 mg/kg CTX was administered for the next 14 days. All rats were superovulated with 150-300 IU/kg pregnant mare serum gonadotropin. 150-300 IU/kg Human chorionic gonadotropin was given, and complete blood counts, bone marrow smears, and spleen sections were examined; the expression of WNT-1, WNT-4, and β-catenin was analyzed. Although the hemoglobin and platelet value in the CTX group was lowest, it was still within the normal reference ranges. The C and S groups had significantly higher white blood cell values (p=0.017). In terms of number of megakaryocytes, Myeloid/ Erythroid ratio, lymphoid cell ratios, no significant differences were found in bone marrow aspiration smears. The CTX group had significantly higher β-catenin expression in the red pulp than the other groups (p=0.0001). The CTX group had the highest WNT-4 expression and very intense expression of WNT-1 in the white pulp. In conclusion, gonadotropins, promising in "treatment", have favorable effects on toxicity of CTX.
{"title":"Effects of Gonadotropins on Blood, Bone Marrow, and Spleen in Cyclophosphamide-Exposed Rats","authors":"","doi":"10.56042/ijeb.v61i11.1764","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1764","url":null,"abstract":"Cyclophosphamide (CTX) is an effective chemotherapeutic agent. Gonadotropins are molecules with various actions. We investigated the effects of gonadotropins on the peripheral blood, bone marrow, and spleen in rats given CTX. Three groups were formed: Control (C) group with no process; Sham (S) group: Physiological saline was applied; CTX group: A single dose of 200 mg/kg and 8 mg/kg CTX was administered for the next 14 days. All rats were superovulated with 150-300 IU/kg pregnant mare serum gonadotropin. 150-300 IU/kg Human chorionic gonadotropin was given, and complete blood counts, bone marrow smears, and spleen sections were examined; the expression of WNT-1, WNT-4, and β-catenin was analyzed. Although the hemoglobin and platelet value in the CTX group was lowest, it was still within the normal reference ranges. The C and S groups had significantly higher white blood cell values (p=0.017). In terms of number of megakaryocytes, Myeloid/ Erythroid ratio, lymphoid cell ratios, no significant differences were found in bone marrow aspiration smears. The CTX group had significantly higher β-catenin expression in the red pulp than the other groups (p=0.0001). The CTX group had the highest WNT-4 expression and very intense expression of WNT-1 in the white pulp. In conclusion, gonadotropins, promising in \"treatment\", have favorable effects on toxicity of CTX.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：