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Evaluation and characterization of TILLING-based putative mutant populations of papaya cv. Arka Prabhath 基于 TILLING 技术的 Arka Prabhath 番木瓜假定突变种群的评估和特征描述
Q4 Agricultural and Biological Sciences Pub Date : 2023-12-30 DOI: 10.58993/ijh/2023.80.4.1
Navya Bhat, D. M R
Manifestation of virus resistance, shelf-life with dwarf stature are important pre-requisites for successful cultivation of papaya in India as it facilitates improved production, productivity and reduced post-harvest loss. One of the methods to create variability is to use mutagens in selected cultivar of papaya. Accordingly, the TILLING-based mutant populations of papaya cv. Arka Prabhath were generated through irradiation of gamma rays ranging from 50 to 500 Gy dosage. In this experiment, the putative mutant populations were evaluated and characterized based on morphological, fruit quality and physiological parameters. Desirable traits such as early flowering, reduced plant height and bearing height, papaya ring spot virus tolerant plants and fruits with extended shelf life were observed in the mutant population. Early flowering and reduced plant height with wide variation was found at 50 Gy wherein the first flowering was on 57 days with 43 cm of plant height in contrast to 80 days in control plants with 79 cm of plant height. Reduced bearing height (40 cm) was observed in 100 Gy with a mean bearing height of 70.65 cm in comparison to control plants (72.97 cm), range being 55 to 115 cm. Further, based on morphological traits twenty-one lines were selected for physiological and fruit quality trait evaluation. Reduction in physiological loss in weight, ethylene evolution rate and respiration rate were observed in the mutant lines treated with 150 Gy dosage. The results advocate the efficacy of gamma induced mutation for developing dwarf cultivar with improved quality and shelf-life of fruits.
抗病毒性、货架期和矮身材是印度成功种植木瓜的重要先决条件,因为这有助于提高产量、生产率和减少收获后的损失。创造变异性的方法之一是在选定的木瓜栽培品种中使用诱变剂。因此,通过 50 至 500 Gy 的伽马射线照射,产生了基于 TILLING 的 Arka Prabhath 木瓜变异种群。在该实验中,根据形态、果实质量和生理参数对推测的突变种群进行了评估和表征。在突变种群中观察到了一些理想的性状,如提早开花、降低株高和生育高度、耐受木瓜环斑病毒植株和延长果实货架期等。在 50 Gy 条件下,早花和株高降低的差异很大,57 天首次开花,株高 43 厘米,而对照植株 80 天首次开花,株高 79 厘米。100 Gy 时,株高降低(40 厘米),平均株高为 70.65 厘米,而对照植株为 72.97 厘米,株高范围为 55 至 115 厘米。此外,还根据形态特征选择了 21 个品系进行生理和果实品质特征评估。经 150 Gy 处理的突变品系在重量、乙烯进化率和呼吸速率方面的生理损失均有所减少。这些结果证明了伽马诱导突变在培育矮化栽培品系方面的功效,能提高果实的质量和货架期。
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引用次数: 0
Development and assessment of biotic stress tolerant cucumber hybrids using land races and commercial varieties 利用陆地品系和商业品种开发和评估耐生物胁迫的黄瓜杂交种
Q4 Agricultural and Biological Sciences Pub Date : 2023-12-30 DOI: 10.58993/ijh/2023.80.4.7
Reena Kumari, Ramesh Kumar, Rajeev Kumar, Aanchal Chauhan, Kumari Shiwani
Cucumber growers suffer significant economic losses due to yield reductions caused by pest such as fruit fly and diseases like powdery mildew, and downy mildew. Therefore, one of the greatest ways to reduce losses caused by these pressures is to grow resistant cultivars. The objective of this study was to estimate important genetic parameters to approximate inbred line performance in the F1 generation, and to identify appropriate F1 cross combinations resistant to these biotic stresses. Thirty F1 hybrid combinations were screened, along with 13 parents (ten diverse stable lines and three testers), and a standard check. The genotypes UHF-CUC-11, UHF-CUC-13, UHF-CUC-6, UHF-CUC-15, UHF-CUC-8, Solan Srijan and F1 cross-combinations UHF-CUC-11 × Solan Srijan, UHF-CUC-13 × Solan Srijan, UHF-CUC-6 × K-75, UHF-CUC-15 × Solan Srijan and UHF-CUC-8 × Solan Srijan were found superior for these biotic stresses in the experimental results. Additionally, the predominance of non-additive gene activity due to genetic complementation between parents suggests that heterosis breeding could be helpful in enhancing these features in cucumber. Therefore, the present study's identification of resistant parents and hybrid combinations can be used to introduce the resistance genes into the necessary elite backgrounds of cucumber for the generation of biotic stress resistant varieties/hybrids.
由于果蝇等害虫和白粉病、霜霉病等病害造成减产,黄瓜种植者蒙受了巨大的经济损失。因此,减少这些压力造成的损失的最佳途径之一就是培育抗性品种。本研究的目的是估算 F1 代近交系表现的重要遗传参数,并确定抗这些生物胁迫的适当 F1 杂交组合。研究人员筛选了 30 个 F1 杂交组合、13 个亲本(10 个不同的稳定品系和 3 个测试品系)以及 1 个标准对照。实验结果表明,基因型 UHF-CUC-11、UHF-CUC-13、UHF-CUC-6、UHF-CUC-15、UHF-CUC-8、Solan Srijan 和 F1 杂交组合 UHF-CUC-11 × Solan Srijan、UHF-CUC-13 × Solan Srijan、UHF-CUC-6 × K-75、UHF-CUC-15 × Solan Srijan 和 UHF-CUC-8 × Solan Srijan 在这些生物胁迫方面表现优异。此外,由于亲本间的遗传互补,非加成基因活动占主导地位,这表明杂交育种有助于提高黄瓜的这些特性。因此,本研究对抗性亲本和杂交组合的鉴定可用于将抗性基因引入必要的黄瓜精英背景,以培育抗生物胁迫的品种/杂交种。
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引用次数: 0
GC-MS based chemical fingerprinting large cardamom cultivars of Sikkim 基于气相色谱-质谱的锡金大豆蔻栽培品种化学指纹图谱分析
Q4 Agricultural and Biological Sciences Pub Date : 2023-12-30 DOI: 10.58993/ijh/2023.80.4.14
Dipak Nayak, Bharat Gudade, Supradip Saha, Khusbu Sharma, Suchisree Jha, Bhumika Pradhan, Prananatha Barman, Tarama Chatterjee5, Arup Mandal
Amomum subulatum Roxb, also known as large cardamom, is an herbaceous plant cultivated in Sikkim, India for its aromatic seeds with diverse applications in food, beverages, perfumery, and medicine. In this study, we aimed at Gas chromatography–mass spectrometry (GC-MS) based chemical fingerprinting through analyzing volatile compounds in seeds and assess the relatedness among seven large cardamom cultivars grown in the Sikkim hill region. We examined the phytochemical composition of large cardamom seed extract using GC/MS analysis. The results indicated significant differences in volatile compounds among the cultivars. The major bioactive components unravelled were 1,8-cineole (76.37-90.63%), α-terpineol (1.73-13.40%), α-terpinolene (0.23-11.51%), β-pinene (0.54-4.13%), γ-terpinolene (0.77-4.47%), and α-pinene (0.35-1.86%), which possess notable antioxidant, anti-inflammatory as well as antibacterial properties. Principal component analysis (PCA) and dendrogram analyses revealed similarity of large cardamom cultivar ICRI SKM-1 with ICRI SKM-2 and Seremna while showed close relatedness among the cultivars such as Ramsey, Ramla, and Sawney based on their phytochemical composition. These findings have important implications for large cardamom breeding programme. The findings of this study also elucidated the potentiality of large cardamom seed extracts for commercial applications in various industries, including medicine, food, and cosmetics.
Amomum subulatum Roxb,又称大豆蔻,是印度锡金种植的一种草本植物,因其种子芳香,可广泛应用于食品、饮料、香水和医药领域。在这项研究中,我们旨在通过分析种子中的挥发性化合物,基于气相色谱-质谱法(GC-MS)进行化学指纹分析,并评估锡金山区种植的七个大豆蔻栽培品种之间的亲缘关系。我们使用 GC/MS 分析方法检测了大豆蔻种子提取物的植物化学成分。结果表明,不同栽培品种的挥发性化合物存在明显差异。所发现的主要生物活性成分为 1,8-松油醇(76.37-90.63%)、α-松油醇(1.73-13.40%)、α-松油烯(0.23-11.51%)、β-蒎烯(0.54-4.13%)、γ-松油烯(0.77-4.47%)和α-蒎烯(0.35-1.86%),这些成分具有显著的抗氧化、抗炎和抗菌特性。主成分分析(PCA)和树枝图分析表明,大豆蔻栽培品种 ICRI SKM-1 与 ICRI SKM-2 和 Seremna 相似,而根据植物化学成分,Ramsey、Ramla 和 Sawney 等栽培品种之间的亲缘关系很近。这些发现对大豆蔻育种计划具有重要意义。这项研究结果还阐明了大豆蔻种子提取物在医药、食品和化妆品等多个行业的商业应用潜力。
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引用次数: 0
Assessment of radiation sensitivity and regeneration in shoot tip culture of banana cv. Grand Naine 评估香蕉品种 Grand Naine 芽尖培养的辐射敏感性和再生能力
Q4 Agricultural and Biological Sciences Pub Date : 2023-12-30 DOI: 10.58993/ijh/2023.80.4.2
Rajni Rajan, Muhammad Feza Ahmad, Awadhesh Pal, Kuldeep Pandey
An investigation was carried out to initiate in vitro mutagenesis in banana cv. Grand Naine shoot tip culture. Based on the sensitivity of explants, the lethal dose was estimated using probit analysis. The LD50 dose of gamma irradiation of the banana shoot tip culture was determined to be 39.5 Gy. It was also discovered that the number of new shoots rose with the number of subculture days, with the largest number of shoots recorded at 40 Gy. The study also discovered that when 13 DAS explants were treated with 10 Gy, they recovered the greatest number of shoots. As a result of the negative effects of higher doses of irradiation, it was decided that determining the lethal dose can be very beneficial in reducing population loss. Its further optimization may be effective in developing significant variations with a certain trait of interest.
对香蕉品种 Grand Naine 的芽尖培养进行了体外诱变调查。根据外植体的敏感性,利用概率分析估算了致死剂量。经测定,伽马射线照射香蕉芽尖培养物的半数致死剂量为 39.5 Gy。研究还发现,新芽的数量随着亚培养天数的增加而增加,在 40 Gy 时记录到的新芽数量最多。研究还发现,用 10 Gy 处理 13 DAS 的外植体时,它们恢复的芽数量最多。由于较高剂量的辐照会产生负面影响,因此确定致死剂量对减少种群损失非常有益。进一步优化该剂量可能会有效地开发出具有某种相关性状的显著变异。
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引用次数: 0
Induced mutations in chrysanthemum through gamma rays 通过伽马射线诱导菊花发生突变
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-25 DOI: 10.58993/ijh/2023.80.3.5
Dharminder Singh, Madhu Bala, Aman Sharma
The research was conducted to induce variations in the chrysanthemum variety ‘Mother Teresa’ in 2020-21. The purpose was to generate and detect the desirable variants concerning altered flower colour, formand flower yield from the treated population. The terminal rooted cuttings were irradiated with different γ-raydoses (0, 5, 10, 15, and 20 Gy) using Cobalt 60 (60Co) as the source and evaluated further along with the controlpopulation, i.e. without irradiations. The observations on various morphological and floral parameters wererecorded and compared with the control population (non-irradiated). Marked floral changes about altered flowercolour, shape, form and changes in leaf shape, colour and sizes were observed with higher doses of gammairradiations. Percent flower colour variations were recorded as 4.10% at 10 Gy, 1.56% at 15 Gy and 4.25% at 20Gy dose. The present study, 11 variants were detected with altered flower colour and form with 10 to 20 Gy-raydoses. The study developed an efficient protocol for the induction of mutations using γ-rays in chrysanthemum.
这项研究是为了在2020-21年诱导菊花品种“特蕾莎修女”的变异。目的是从处理过的群体中产生和检测有关改变花色、形状和花产量的理想变异。以钴60 (60Co)为源,用不同的γ射线剂量(0、5、10、15和20 Gy)照射末端根插条,并与对照种群(即不照射)一起进一步评估。记录了各种形态和花卉参数的观察结果,并与未辐照的对照群体进行了比较。在较高剂量的γ射线照射下,花的颜色、形状、形态以及叶的形状、颜色和大小都发生了明显的变化。在10 Gy、15 Gy和20Gy剂量下,花的颜色变化率分别为4.10%、1.56%和4.25%。在目前的研究中,用10到20次射线剂量检测到11种变异,其花色和形状发生了变化。本研究开发了一种利用γ射线诱导菊花突变的有效方案。
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引用次数: 0
Assessment of genetic parameters in periwinkle through diallel analysis 用双列分析评价长春花的遗传参数
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-25 DOI: 10.58993/ijh/2023.80.3.6
None Bolagam Ravikumar, K. K. Dhatt
Estimation of genetic variability assists in determining the contribution of various plant parameters inexhibiting the improvement of genotypes under a specific set of environmental factors. Genetic analysis allows us to clearly understand various morphological, physiological, and genetic traits, in addition to the type and extent of their role in the advancement of cultivars. The present investigation was carried out to evaluate six inbred lines of periwinkle (Vi-15-1, Vi-13-2, Vi-16, Vi-15-2, Vi-29, Vi-14-3), and their cross combinations in the full diallel fashion for ornamentally important characters. Analysis of variance revealed highly significant variations for all the parameters in all the inbred lines utilized in the study. The highest broad sense heritability estimates were observed for the number of seeds/follicles (H= 97.77 %) in the parents, whereas plant height had the highest heritability in the cross combinations (H= 98.73%). The high values of phenotypic and genotypic coefficient of variation in parents (PCV=34.41; GCV=34.02) and crosses (PCV=25.49; GCV=24.93) were recorded for thenumber of seeds/follicles. In the present study, moderate to high heritability together with a high percentage of genetic advance was recorded for seeds/follicle, follicle length, primary branches/plant, plant height, internode length and plant spread, suggesting that the additive gene action controlled these characters. Therefore, the results suggested the existence of variability for studied traits in these periwinkle inbred lines, which should be improved in future breeding.
遗传变异的估计有助于确定在特定环境因素下各种植物参数对基因型改善的贡献。遗传分析使我们能够清楚地了解各种形态、生理和遗传性状,以及它们在品种进步中的作用的类型和程度。对长春花6个自交系(Vi-15-1、Vi-13-2、Vi-16、Vi-15-2、Vi-29、Vi-14-3)及其观赏重要性状的全双列杂交组合进行了评价。方差分析表明,本研究利用的所有自交系各参数的变异均极显著。在杂交组合中,种子/卵泡数的广义遗传力最高(H= 97.77%),而株高的广义遗传力最高(H= 98.73%)。亲本表型变异系数和基因型变异系数较高(PCV=34.41;GCV=34.02)和杂交(PCV=25.49;GCV=24.93),记录种子/卵泡数。在本研究中,种子/毛囊、毛囊长度、一次枝/株、株高、节间长和株距均表现出中至高的遗传力和较高的遗传超前率,表明加性基因作用控制了这些性状。因此,这些长春花自交系所研究的性状存在变异,应在今后的育种中加以改进。
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引用次数: 0
Optimization of in vitro propagation of jamun variety Konkan Bahadoli jamun品种Konkan Bahadoli离体繁殖的优化
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-25 DOI: 10.58993/ijh/2023.80.3.3
Harsimran Kamboj, Krishan Kumar, J. S. Brar, Anil Kumar, Parshotam Kumar (PK) Arora
Micro-propagation is a vital technique for round-the-year clonal multiplication of plants. Jamun is anindigenous fruit tree with great pharmacological importance. Two explants viz., shoot tips and nodal explants were cultured from January to May. The shoot regeneration from nodal explants was tested using 6-benzyl amino purine (BAP; 1.0-4.0 mg l-1) and α-naphthalene acetic acid (NAA; 0-0.25 mg l-1), while rooting was investigated with NAA (0.5-2.0 mg l-1). Maximum explant establishment was recorded in May. The nodal explants showed better establishment response (50.0%) than shoot tips (40.7%). The maximum shoot regeneration (72.16%) and earliest evocation response from nodal segments (14.0 days) were obtained with combinations of BAP (2.0 mg l-1) and NAA (0.25 mg l-1). Silver nitrate (AgNO3 @ 3.0 mg l-1) enabled shoot proliferation. Maximum rooting occurred on the medium containing 0.5 mg l-1 NAA. The in vitro plants were hardened on the potting mixture of cocopeat (4): perlite (1): vermiculite (1).
微繁技术是实现植物无性系全年繁殖的重要技术。Jamun是一种具有重要药理意义的本土果树。1 ~ 5月分别培养茎尖和节状外植体。采用6-苄基氨基嘌呤(BAP;1.0-4.0 mg l-1)和α-萘乙酸(NAA;0 ~ 0.25 mg l-1), NAA (0.5 ~ 2.0 mg l-1)对生根的影响较大。外植体建立在5月份达到最大值。结节外植体的建立响应(50.0%)优于茎尖(40.7%)。BAP (2.0 mg l-1)和NAA (0.25 mg l-1)组合处理的芽再生率最高(72.16%),节段唤起反应最早(14.0 d)。硝酸银(AgNO3 @ 3.0 mg l-1)使芽增殖。在含有0.5 mg l-1 NAA的培养基上生根率最高。在椰泥(4):珍珠岩(1):蛭石(1)的盆栽混合物上硬化离体植株。
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引用次数: 0
Improvement of ginger for yield and quality traits under the hill ecosystem of Mizoram 米佐拉姆邦丘陵生态系统下生姜产量和品质性状的改良
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-25 DOI: 10.58993/ijh/2023.80.3.8
J. K. Soni, None Vishambhar Dayal, S. K. Sunani, Lalramhlimi Bawitlung, Amit Kumar, Ingudam Shakuntala, Sunil Doley
The field study on ginger was carried out at ICAR Research Farm, Mizoram Centre, for three consecutive years (2019-2021). The best selection criteria for crop improvement were determined based on genetic component analyses and Principal Component Analysis (PCA). There exists highly significant variation for all traits due to genotypes based on analysis of variance. The highest extent of variability was recorded in oleoresin content. The magnitude of heritability and genetic gain was moderate to high for LAI, leaves per hill, tillers per plant, fresh weight of clump, rhizomes per plant, dry recovery, total chlorophyll, total phenol content, 6-gingerol and oleoresin content, indicating the additive gene control for which choosing of these traits will be beneficial. The rhizome yield exhibited the highest positive correlation with leaves per hill, followed by LAI and tillers per plant. Direct selection of characters like LAI, fresh weight of clump, rhizomes per plant and 6-gingerol content could be most reliable for yield improvement. Based on PCA and bi-plot figures, LAI, tillers per plant, leavesper hill, rhizomes per plant, fresh weight of clump, and yield of rhizomes contributed mostly to genetic diversity among the ginger genotypes and ‘Bold Nadia’, ‘Bhaise’ and ‘PGS 102’ were identified as the best genotypes possessing optimum combinations of yield and quality traits for exploitation in future programmes under Mizoram condition
生姜的实地研究连续三年(2019-2021年)在米佐拉姆中心ICAR研究农场进行。通过遗传成分分析和主成分分析,确定了作物改良的最佳选择标准。方差分析表明,各性状因基因型不同而存在极显著差异。最大程度的变化记录在油树脂含量。叶面积指数、单山叶数、单株分蘖数、丛枝鲜重、单株根状茎数、干恢复、总叶绿素、总酚含量、6-姜辣素和油树脂含量的遗传力和遗传增益均为中高,说明这些性状的选择有利于加性基因控制。根茎产量与单山叶数呈显著正相关,其次是叶面积指数和单株分蘖数。直接选择叶面积指数、植株鲜重、单株根茎数和6-姜辣素含量是提高产量最可靠的指标。通过主成分分析和双图分析发现,LAI、单株分蘖数、每株叶数、单株根状茎、茎丛鲜重和根状茎产量对不同生姜基因型的遗传多样性贡献最大,其中‘Bold Nadia’、‘Bhaise’和‘PGS 102’是未来米佐拉姆条件下产量和品质性状组合最优的基因型
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引用次数: 0
Tractor-operated hydraulically controlled tree shaker for harvesting fruits 拖拉机操作的液压控制果树采收机
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-25 DOI: 10.58993/ijh/2023.80.3.10
Apoorv Prakash, A. K. Dixit, Rohinish Khurana, Manjeet Singh, A. K. Mahal, G. S. Manes
A tractor-operated hydraulically controlled tree shaker was developed to harvest Aonla (Phyllanthusemblica) and Jamun (Syzygium cumini) fruits. This machine generates vibration to shake fruit trees withprovision to hold the tree trunk up to 2438 mm high. Besides, a collecting basket made of green net with fully opened diameter of 6706 mm has also been developed. Performance assessment of the machine was carried out on Aonla and Jamun fruit trees at two different stroke rates and compared with the traditional method of fruit harvesting. The best results were obtained at stroke rates 1480 ± 8 and 1600 ± 12 rpm for Aonla and Jamun trees, respectively. The average harvest per tree at these stroke rates was 52.99 kg and 12.53 kg for Aonla and Jamun trees, respectively. Mature, immature, and damaged harvested fruits were 78.59%, 5.59% and 15.81%, respectively, for Aonla. In Jamun, the mature, immature, and damaged harvests were 82.34%, 7.10%, and 10.56%, respectively. The overall fuel consumption for harvesting Aonla and Jamun fruits was 5.6 and 6.06 l/h, respectively. For Aonla, the cost of harvesting with the machine was Rs. 5.03/kg, whereas it was Rs. 20.94/kg for jamun. In contrast, the cost of harvesting in the traditional method was Rs. 4.20/kg for Aonla and Rs. 15/kg for Jamun. The cost of operation was higher due to the harvesting of untrained, non-pruned treesand the machine being a functional prototype. The overall saving in labour was 74-80% in Aonla and 75-80% in Jamun fruit harvesting compared to the manual method
研制了一种拖拉机操作的液压摇树机,用于采收甘树树(Aonla)和甘树树(Jamun)果实。本机产生振动,使果树摇晃,使树干高度达到2438毫米。此外,还研制出了全开口直径6706 mm的绿网收集篮。对Aonla和Jamun果树在两种不同的冲程速率下进行了性能评估,并与传统的水果采收方法进行了比较。Aonla和Jamun树在冲程速率分别为1480±8和1600±12 rpm时获得最佳效果。Aonla树和Jamun树在这些中风率下的平均每棵收获量分别为52.99公斤和12.53公斤。成熟果、未成熟果和破损果的采收率分别为78.59%、5.59%和15.81%。在贾门,成熟、未成熟和受损收获率分别为82.34%、7.10%和10.56%。采收Aonla和Jamun果实的总油耗分别为5.6和6.06 l/h。对于Aonla来说,用机器收割的成本是5.03卢比/公斤,而jamun的成本是20.94卢比/公斤。相比之下,Aonla和Jamun的传统收割成本分别为每公斤4.20卢比和15卢比。由于采伐的是未经训练、未经修剪的树木,而且这台机器只是一个功能原型,因此操作成本较高。与手工方法相比,Aonla和Jamun水果采收的总体劳动力节约分别为74-80%和75-80%
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引用次数: 0
Morphological and anatomical studies of pollen in peaches 桃花粉的形态学和解剖学研究
Q4 Agricultural and Biological Sciences Pub Date : 2023-09-25 DOI: 10.58993/ijh/2023.80.3.2
Rajender Kumar, D. C. Dimri, Nikesh Chandra, Jitendra Singh, Shivran ., Sampurna Nand Singh
Analysis of the morphology and ultrastructure of pollen grains using scanning electron microscopy (SEM)has shown to be an effective method for identifying related genotypes of peach. The micromorphology of pollengrains of 15 peach genotypes using scanning electron microscopy (SEM) was studied. The anther colour ofthe accessions examined differed considerably, varying from red (11 Nos), dark red (Selection-12),creamishwhite (Sharbati Late), purple (Selection-1) and yellow (Selection-2) in colour. All the accessions and cultivarsstudied showed tricolporated pollens. Pant Peach-1 had the longest pollen (48.68 μm), and Sharbati Late hadthe shortest pollens (34.44 μm). The highest pollen width was noticed in IC-360680 (38.64 μm) and minimumin Sharbati Late (24.94 μm). All the peach cultivars displayed striate and tectum perforatum ornamentationregarding the surface features. Striae width ranged between 0.25 μm (Pratap) to 0.55 μm (IC-360680), while thehighest groove width was noted in Pant Peach-1 (0.60 μm) and mi nimum in Selection-2 (0.19 μm).
利用扫描电镜(SEM)分析花粉粒的形态和超微结构是鉴定桃树相关基因型的有效方法。利用扫描电镜(SEM)对15个桃基因型花粉的微观形态进行了研究。所检查的材料的另一种花药颜色差异很大,从红色(11个),暗红色(选择-12),奶油白色(Sharbati Late),紫色(选择-1)和黄色(选择-2)的颜色不同。所研究的所有材料和栽培物均显示三聚花粉。粉桃1号的花粉最长(48.68 μm),晚茶最短(34.44 μm)。IC-360680花粉宽度最大(38.64 μm), Sharbati Late花粉宽度最小(24.94 μm)。所有品种桃的表面特征均为条纹状和贯叶顶状纹饰。条纹宽度在0.25 μm (Pratap)到0.55 μm (IC-360680)之间,而槽宽最大的是Pant Peach-1 (0.60 μm),最小的是Selection-2 (0.19 μm)。
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引用次数: 0
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Indian Journal of Horticulture
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