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Detection and Phylogenetic Analysis of Wolbachia in Onion Thrips (Thrips tabaci Lindeman) 洋葱蓟马(Thrips tabaci Lindeman)体内沃尔巴克氏体的检测和系统发育分析
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.963
P. Randive, Pravin Khambalkar, K. Khandagale, Indira Bhangare, K. Chandrashekhar, Major Singh, Suresh Gawande
Wolbachia is a group of bacteria that is known to infect many arthropods and nematodes. It is one of the most common parasitic microbes and is possibly the most common reproductive parasite in the biosphere. The bacterium is best known for its ability to manipulate host reproductive biology where it can induce cytoplasmic incompatibility, parthenogenesis, feminization and male-killing. In the present study, onion thrips (Thrips tabaci Lindeman) were collected from 9 locations in India along with melon thrips (Thrips palmi Karny) and chilli thrips (Scirtothrips dorsalis). From the molecular level detection by using 16s rDNA, the Wolbachia infection has been detected in the onion thrips collected from 6 locations out of 9. Melon thrips and chilli thrips samples were also found to be infected by Wolbachia. The phylogenetic analysis revealed that all detected Wolbachia samples showed that all were distantly related to the previously known Wolbachia samples.
沃尔巴克氏菌是一类已知可感染许多节肢动物和线虫的细菌。它是最常见的寄生微生物之一,也可能是生物圈中最常见的生殖寄生虫。这种细菌最著名的能力是操纵宿主的生殖生物学,它可以诱导细胞质不相容、孤雌生殖、雌性化和杀雄。本研究从印度 9 个地点收集了洋葱蓟马(Thrips tabaci Lindeman)以及瓜蓟马(Thrips palmi Karny)和辣椒蓟马(Scirtothrips dorsalis)。通过使用 16s rDNA 进行分子水平检测,从 9 个地点中的 6 个地点采集的洋葱蓟马中检测到了沃尔巴克氏体感染,瓜蓟马和辣椒蓟马样本也被发现感染了沃尔巴克氏体。系统进化分析表明,所有检测到的沃尔巴克氏体样本都与之前已知的沃尔巴克氏体样本关系密切。
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引用次数: 0
Protocol for Temperature Toxicity Investigation on Whitefly Bemisia tabaci (Gennadius) 温度对烟粉虱(Gennadius)的毒性调查规程
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.1355
Nikhil R M, Anil Kumar S T, Subramanian S, M. G K
A cost-effective laboratory protocol was perfected for investigating temperature toxicity against whitefly Bemisia tabaci (Genn.) on tomato. Understanding temperature toxicity relationship will aid in strategization of pest management. The experiments conducted to develop the current protocol used B. tabaci Asia II-1 as test insect. Essentially this protocol consist of three steps which are: (1) Temperature incubation of test insects: includes three hour starvation period and temperature treatment of B. tabaci; (2) Preparatory steps of leaf-dip bioassay: suggests use of agar cube method (reduces agar usage by 70%) to maintain green and turgid test leaves during post-exposure period of bioassay; (3) Release of temperature treated insects and mortality counting: test insect release, securing petri plates, recording observations, etc. all are deliberated in detail. This protocol facilitates economical, convenient and easy-to-handle experimentation to study temperature toxicity relationship in B. tabaci.
为研究温度对番茄上粉虱(Bemisia tabaci (Genn.))的毒性,完善了一套经济有效的实验室方案。了解温度毒性关系有助于制定害虫管理战略。为制定当前方案而进行的实验使用了亚洲 II-1 号烟粉虱作为试验昆虫。该方案主要包括三个步骤,即(1) 试验昆虫的温度培养:包括三小时的饥饿期和 B. tabaci 的温度处理;(2) 浸叶生物测定的准备步骤:建议使用琼脂立方体法(可减少 70% 的琼脂用量),以在生物测定暴露后期间保持试验叶片的绿色和活力;(3) 释放经温度处理的昆虫并计算死亡率:对释放试验昆虫、固定培养皿、记录观察结果等进行了详细讨论。该实验方案经济、方便、易于操作,可用于研究烟青虫的温度毒性关系。
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引用次数: 0
Physical Attributes of Wheat Varities in Relation to Rhyzopertha dominica (F.) 小麦变种的物理特性与 Rhyzopertha dominica (F.) 的关系
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.1652
Ramalakshmi V, C. R. Satapathy, S. Borkataki, Soumik Ray
This study aims to explore the susceptibility of wheat varieties in storage against Rhyzopertha dominica (F.). Twenty promising wheat cultivars were tested for resistance in non-choice conditions at the Department of Entomology, MSSSoA, Odisha during 2021-22. The wheat varieties were premeditated for their physical components through correlation analysis and the level of influence on insect preference and development was determined. The variety DBW303 which had a high median development period, low adult emergence and susceptible index was found to be resistant. Similarly, the variety PBW373 which recorded a low median developmental period with higher adult emergence, and susceptible index, was found to be susceptible. Among the physical traits, varieties with grain hardness exhibited a negative relationship with progeny development and thus conferred resistance.
本研究旨在探讨小麦品种在储藏过程中对 Rhyzopertha dominica (F.) 的敏感性。2021-22 年期间,在奥迪沙邦 MSSSoA 昆虫学系对 20 个有前途的小麦品种进行了非选择条件下的抗性测试。通过相关分析对小麦品种的物理成分进行了预选,并确定了其对昆虫喜好和发展的影响程度。结果发现,中位发育期长、成虫出现率低、易感指数低的品种 DBW303 具有抗性。同样,品种 PBW373 的中位发育期较低,成虫出现率和易感指数较高,被认定为易感品种。在物理性状中,谷粒硬度与后代发育呈负相关,因此具有抗性。
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引用次数: 0
A Novel Damage Intensity Index for Tea Mosquito Bug Helopeltis spp. Infestation in Cocoa 可可中茶叶蚊虫 Helopeltis spp.
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.1555
S. Thube, R. Pandian, C. T. Jose, Bhavishya A, Santhosh Kumar Paichal, O. Navik, Akash Nikoshe, G. Mahapatro
The present investigation focused on the development of a new damage intensity index (DII) for the tea mosquito bug Helopeltis spp. (TMB) infestation in cocoa. The new DII was developed based on observable symptoms and the intensity of damage, such as the number and diameter of feeding lesions, and TMB-infested areas on cocoa pods. Statistical techniques, including linear correlation and regression, have been employed to develop an appropriate DII. The study reveals a significant correlation between the mean of total feeding lesions (Xn) and feeding lesions present on the middle portion (Xm) of cocoa pods, surpassing other combinations and indicating a strong relationship between these variables. The damage intensity was estimated using a linear regression approach resulting equation, Xn = 1.79 + 0.25Mn (R2 = 0.989), offers a quantified means of estimating damage intensity on cocoa pods. Analysis of infestation and the regression equation establishes DII values between 1.79 to 76.79. This novel index suggests that the minimum sample size of 90 pods is required for estimating a population with a 95% confidence level to arrive at precision decision making of TMB damage on cocoa pods. The novel damage intensity index and the calculated sample size from this investigation could be useful in conducting various studies, such as germplasm screening, breeding for pest resistance, evaluating the effectiveness of new insecticides, and formulating an IPM package for managing Helopeltis spp. in cocoa.
本调查的重点是针对可可中的茶蝽属(TMB)虫害制定新的危害强度指数(DII)。新的损害强度指数是根据可观察到的症状和损害强度(如取食病斑的数量和直径)以及可可荚果上受 TMB 侵染的面积制定的。采用了线性相关和回归等统计技术来制定适当的 DII。研究显示,可可荚上总食害(Xn)和中间部分食害(Xm)的平均值之间存在明显的相关性,超过了其他组合,表明这些变量之间关系密切。利用线性回归法估算损害强度,得出方程 Xn = 1.79 + 0.25Mn (R2 = 0.989),为估算可可荚上的损害强度提供了量化方法。虫害分析和回归方程确定的 DII 值在 1.79 到 76.79 之间。这一新颖的指数表明,在 95% 的置信水平下,最少需要 90 个可可荚的样本量才能估算出一个种群,从而对 TMB 对可可荚的危害做出精确的决策。这项调查得出的新型危害强度指数和计算出的样本量可用于开展各种研究,如种质筛选、害虫抗性育种、评估新型杀虫剂的效果以及制定可可褐飞虱综合防治方案。
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引用次数: 0
Impact of Essential Oils on Biological Traits of Trichogramma chilonis Ishii 精油对石井毛蟹生物性状的影响
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.1521
Sowmya M, B. O. S., K. Subaharan, Vinay Kumar T M, Soundarya Y M, V. R., Navik O, Amala U, Sushil S N
Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) reared on factious host rice moth Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) is an egg parasitoid on lepidopteran pests. The essential oils, viz., ajowan Trachyspermum ammi, betel Piper betle, and sweet basil Ocimum basilicum and neem oil were assessed for their toxicity and biological traits like parasitism and emergence on T. chilonis. Among the EOs tested, T. ammi caused highest toxicity to T. chilonis (LC50 0.316 ppm) followed by P. betle (LC50 0.567 ppm). At sublethal dose, they had an impact on parasitism and adult emergence of T. chilonis. Whilst O. basilicum and neem oil were relatively safe at sublethal doses to T. chilonis. This study highlights the need to maintain a waiting period before the release of T. chilonis in EOs treated substrates.
寄生在事实性寄主米蛾 Corcyra cephalonica (Stainton) (鳞翅目:俾拉尔科)上的 Trichogramma chilonis Ishii(膜翅目:Trichogrammatidae)是鳞翅目害虫的卵寄生虫。本研究评估了矢车菊、蒌蒿、甜罗勒和印楝油等精油对鳞翅目害虫的毒性和生物特性,如寄生性和萌发性。在测试的环氧乙烷中,安米对褐飞虱的毒性最高(LC50 0.316 ppm),其次是槟榔(LC50 0.567 ppm)。在亚致死剂量下,它们会影响 T. chilonis 的寄生和成虫出现。而罗勒草和印楝油在亚致死剂量下对 T. chilonis 相对安全。这项研究强调,在释放经环氧乙烷处理过的基质中的褐飞虱之前,需要保持一段等待期。
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引用次数: 0
Morphometrics of Intraspecific Populations of Stingless Bee Tetragonula iridipennis Smith from Southern Tamil Nadu 泰米尔纳德邦南部无刺蜂 Tetragonula iridipennis Smith 种内种群的形态计量学
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.1651
K. Balaji, J. Jayaraj, M. Shanthi, S. Vellaikumar, C. Rajamanickam, N. Chitra, K. Suresh
Stingless bees also referred to as meliponine bees and morphological characteristics of these differ depending on are the species and environmental kinds. The current study explores morphology and morphometrics of Tetragonula iridipennis Smith in Tamil Nadu, India. The morphometrics of worker bees were compared over locations. The bees captured in the Kolli hills were significantly larger than those collected elsewhere. The mean value of head length (HL) (1.43 mm), head width (HW) (1.68 mm), antennal length (AL) (1.98 mm), hind leg length (HLL) (3.54 mm) and forewing length (FL) (3.84 mm) were recorded. The results revealed variance of four principal components (PC) reaching 82.4%, as (PC1) 38.58%, (PC2) 20.95%, (PC 3) 19.75% and (PC4) 8.10%. The characters of FL, HW, FW (forewing width), HLL, AL, OOD (ocello ocular distance), HTW (hind tibial width) and HL were the most discriminative in the principal component analysis.
无刺蜂也被称为蜜蜂(meliponine bees),其形态特征因种类和环境而异。本研究探讨了印度泰米尔纳德邦 Tetragonula iridipennis Smith 的形态和形态计量学。研究人员比较了不同地点工蜂的形态特征。在科利山采集到的工蜂明显比在其他地方采集到的工蜂大。记录的平均值包括头长(HL)(1.43 毫米)、头宽(HW)(1.68 毫米)、触角长(AL)(1.98 毫米)、后腿长(HLL)(3.54 毫米)和前翅长(FL)(3.84 毫米)。结果显示,四个主成分(PC)的方差达到 82.4%,分别为(PC1)38.58%、(PC2)20.95%、(PC3)19.75% 和(PC4)8.10%。在主成分分析中,FL、HW、FW(前翅宽)、HLL、AL、OOD(眼距)、HTW(后胫宽)和 HL 的特征最具区分度。
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引用次数: 0
Influence of Potassium Fertilization on the Incidence of Sucking Insect Pests on Cucumber 钾肥对黄瓜吸浆虫害发生率的影响
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.1419
A. Wahsh, Samir S. Awadalla, H. El-Serafi, Magdy A. El-Hariry
Experiments were conducted in Fayoum Governorate, to study the effect of potassium (K) fertilization on the incidence of Aphis gossypii (Glover), Bemisia tabaci (Gennadius), Nezara viridula (L), Phenacoccus solenopsis (Tinsley), Thrips tabaci (Lindeman), Myzus persicae (Sulzer), Empoasca decedens (Paoli), and Empoasca decipiens (Paoli) in cucumber during March planting season of 2018 and 2019. Three levels of potassium (25, 50 and 75 kg/ feddan) were used with a fertilizer containing 24% K2O. The control had no potassium (0 kg). The incidence rates of all the pests was observed to be maximum with nil potassium (0 kg) fields (30.8% and 30.6%). The least incidence was observed with 75 kg potassium (19.3% and 19.1%) indicating that increased potassium reduced incidence of some sucking pests.
在法尤姆省进行了一项试验,研究施用钾肥对棉蚜(Glover)、烟粉虱(Gennadius)、蚜茧蜂(L)、蓟马(Lindeman)、柿螨(Sulzer)和蚜虫(Empoasca decens (Paoli))发病率的影响、Phenacoccus solenopsis (Tinsley)、蓟马(Lindeman)、Myzus persicae (Sulzer)、Empoasca decedens (Paoli) 和 Empoasca decipiens (Paoli)在 2018 年和 2019 年 3 月种植季节黄瓜上的发生率。使用了三种钾含量(25、50 和 75 千克/亩)的肥料,其中 K2O 含量为 24%。对照组不施钾(0 千克)。据观察,零钾(0 千克)田块的害虫发生率最高(30.8% 和 30.6%)。钾含量为 75 千克的田块虫害发生率最低(分别为 19.3% 和 19.1%),这表明钾的增加降低了一些吸浆害虫的发生率。
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引用次数: 0
Seed Treatment with Insecticides- A Potential Option against Fall Army Worm Spodoptera frugiperda in Maize 用杀虫剂进行种子处理--防治玉米中的秋陆虫(Spodoptera frugiperda)的潜在选择
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.1329
Rakesh Kumar Behera, Murali Mohan K, P. Ganiger
Spraying with insecticides is generally carried out for the management of fall army worm Spodoptera frugiperda (J E Smith) in maize. As the pest causes significant damage in the vegetative stages, seed treatment can also be an option. In the present study, seed treatment has been evaluated for its efficacy in comparison with spray in maize. The results suggested that chlorantraniliprole 62.5FS was more effective than presently used seed treatment with cyantraniliprole. In case of foliar spray, spinetoram 11.7SC along with chlorantraniliprole 18.5SC and emamectin benzoate 5SG were found effective. As seed treatment is easy to practice and economical, seed treatment with diamides, particularly chlorantraniliprole 62.5FS might provide a better alternative to foliar sprays during early crop stage. Hence, an integrated strategy involving seed treatment in combination with need-based foliar spray at later crop stages may make management of S. frugiperda more efficient.
喷洒杀虫剂通常是为了防治玉米上的秋虫 Spodoptera frugiperda (J E Smith)。由于该害虫会在无性阶段造成严重危害,种子处理也是一种选择。在本研究中,对玉米种子处理与喷雾的效果进行了比较评估。结果表明,氯虫苯甲酰胺 62.5FS 比目前使用的氰戊菊酯种子处理剂更有效。在叶面喷洒方面,发现辛硫磷 11.7SC、氯虫苯甲酰胺 18.5SC 和阿维菌素苯甲酸盐 5SG 均有效。由于种子处理简单易行且经济实惠,在作物早期阶段,使用二酰胺类药物,特别是氯虫苯甲酰胺 62.5FS 进行种子处理,可能会成为叶面喷洒的更好替代品。因此,在作物后期采用种子处理与根据需要进行叶面喷洒相结合的综合策略,可能会更有效地防治蚜虫。
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引用次数: 0
Biorational Management of Major Chewing Pests of Okra 秋葵主要咀嚼害虫的生物治理
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.1360
Gagan B. S., B. Ramesha, K. M. Sreekumar, A. S. S., Manju Mary Paul
Field experiments were conducted to evaluate the efficacy of microbial agents, and neem based and biorational insecticides against major lepidopteran pests of okra, viz., shoot and fruit borer Earias vitella (F) and leaf roller Haritalodes derogata (F) during summer and kharif 2022. The experiment was laid out in randomized block design (RBD) with nine treatments and three replications. Results indicated that spinosad 45SC was the most effective with highest marketable yield. Thus, spinosad 45SC can be used for the effective management of E. vitella and H. derogate in okra.
田间试验旨在评估微生物制剂、印楝类杀虫剂和生物杀虫剂对黄秋葵主要鳞翅目害虫(即嫩枝和果实蛀螟Earias vitella(F)和卷叶蛾Haritalodes derogata(F))的防治效果。试验采用随机区组设计(RBD),共设 9 个处理和 3 次重复。试验结果表明,spinosad 45SC最有效,可销售产量最高。因此,可将 spinosad 45SC 用于有效防治黄秋葵中的葡萄疫霉菌(E. vitella)和减损疫霉菌(H. derogate)。
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引用次数: 0
Pollinator Diversity and Pollination Efficiency of Stingless Bee Tetragonula iridipennis Smith in Bitter Gourd 无刺蜂 Tetragonula iridipennis Smith 在苦瓜中的传粉者多样性和传粉效率
Pub Date : 2023-10-03 DOI: 10.55446/ije.2023.1650
K. Balaji, J. Jayaraj, M. Shanthi, S. Vellaikumar, C. Rajamanickam, N. Chitra, K. Suresh
An experiment was conducted out to investigate the pollinator diversity and pollination efficiency of Tetragonula iridipennis Smith on bitter gourd (Momordica charantia L.). In this study, there were 16 floral visitor species were recorded. Among them, six species were the most frequent visitors. A highest mean of visits was recorded with Halictus sp. (7.80) during 1000-1200 hrs in relative abundance and the lowest was of Megachile sp. (2.90) at 1600-1800 hrs. The foraging rate of stingless bees was most active in morning 1000-1200 hrs. The maximum fruit set was observed in pollinator-imposed fields (80.23 %) compared to pollinator excluded fields (71.15 %). Prominent increase in fruit length and girth in the pollinator included plots were respectively observed to be 249.19 and 42.91 mm. Maximum number of fruit/ individual plant (24.10) was achieved with bee pollination as compared to control (21.20).
研究人员进行了一项实验,以调查史密斯四角瓢虫(Tetragonula iridipennis Smith)对苦瓜(Momordica charantia L.)的授粉多样性和授粉效率。在这项研究中,共记录了 16 种访花者。其中,6 个物种是最频繁的访花者。在相对丰度方面,Halictus sp.的平均访花次数最高(7.80),为 1000-1200 hrs;Megachile sp.的平均访花次数最低(2.90),为 1600-1800 hrs。无刺蜂的觅食活动在上午 10 时至 12 时最为活跃。授粉田的坐果率最高(80.23%),而不授粉田的坐果率为 71.15%。在有授粉昆虫的地块中,果实的长度和周长显著增加,分别为 249.19 毫米和 42.91 毫米。与对照组(21.20)相比,蜜蜂授粉的单株果实数量最多(24.10)。
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引用次数: 0
期刊
Indian journal of entomology
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