Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864564
Li Yu, Hui-jun Xu, Su-jing Zhang
To improve the coverage of irradiation on tumor and resolve the problem of dose shortage at the edge of tumor beside sensitive organs, we will discuss about designing divisional target plans for massive liver cancer. On the stage of designing treatment plans, we divide the Planning Target Volume (PTV) into three sub-targets: PTV1, PTV2 and PTV3. PTV1 and PTV3 are the regions for designing treatment plans, and PTV2 is the spacer region between PTV1 and PTV3. We select two cases of massive liver cancer, adopt the same prescribed dose and dose threshold of sensitive organs, and then design plans for a single target and divisional targets respectively. We will compare and evaluate the results of these two methods. For case 1, the tumor coverage rates of a single target plan and divisional target plan were 66.51% and 99.00%, respectively. For case 2, the tumor coverage rates of a single target plan and divisional target plan were 56.85% and 92%. Through divisional target plans, the tumor coverage of case 1 and 2 improves 32.49% and 35.15%, respectively. So the design of divisional target plan greatly improves the coverage rate of irradiation on tumor, but prolongs the time of treatment.
{"title":"Compare the design of divisional target plan and single target plan for massive liver cancer","authors":"Li Yu, Hui-jun Xu, Su-jing Zhang","doi":"10.1109/ICMIPE.2013.6864564","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864564","url":null,"abstract":"To improve the coverage of irradiation on tumor and resolve the problem of dose shortage at the edge of tumor beside sensitive organs, we will discuss about designing divisional target plans for massive liver cancer. On the stage of designing treatment plans, we divide the Planning Target Volume (PTV) into three sub-targets: PTV1, PTV2 and PTV3. PTV1 and PTV3 are the regions for designing treatment plans, and PTV2 is the spacer region between PTV1 and PTV3. We select two cases of massive liver cancer, adopt the same prescribed dose and dose threshold of sensitive organs, and then design plans for a single target and divisional targets respectively. We will compare and evaluate the results of these two methods. For case 1, the tumor coverage rates of a single target plan and divisional target plan were 66.51% and 99.00%, respectively. For case 2, the tumor coverage rates of a single target plan and divisional target plan were 56.85% and 92%. Through divisional target plans, the tumor coverage of case 1 and 2 improves 32.49% and 35.15%, respectively. So the design of divisional target plan greatly improves the coverage rate of irradiation on tumor, but prolongs the time of treatment.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115180956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864563
Li Wei, Yan Zhiqiao, Huang Yue, Xu Jiang
Spectrum mapping reflects the strength of brain electrical activity in different frequency band. The current study analyzes changes in electroencephalography (EEG) after spinal cord injury (SCI) on the basis of spectrum mapping. Comparing spectrum mappings of SCI patients and normal subjects, the fact that beta rhythm dominated in brain electrical activity during walking period was concluded. In addition, the proportion of delta wave increased and slightly exceeded that of beta wave after spinal cord injury and power values in delta band of SCI patients performed greater than that of normal subjects.
{"title":"Changes in spectrum mapping in rhythmic movement involved in walking after spinal cord injury","authors":"Li Wei, Yan Zhiqiao, Huang Yue, Xu Jiang","doi":"10.1109/ICMIPE.2013.6864563","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864563","url":null,"abstract":"Spectrum mapping reflects the strength of brain electrical activity in different frequency band. The current study analyzes changes in electroencephalography (EEG) after spinal cord injury (SCI) on the basis of spectrum mapping. Comparing spectrum mappings of SCI patients and normal subjects, the fact that beta rhythm dominated in brain electrical activity during walking period was concluded. In addition, the proportion of delta wave increased and slightly exceeded that of beta wave after spinal cord injury and power values in delta band of SCI patients performed greater than that of normal subjects.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116046389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864503
Xin Xiong, Lisheng Xu, Baolin Mao, Xiaozhao Chen, Yan Kang
A fast and robust colorectal polyp detection framework in CT colonography was proposed. In order to speed the detection of polyp in CT colonography, a cascade-Adaboost framework was employed, and a lot of candidates were rejected quickly in the first stages of the cascade framework. To improve the performance of cascade-Adaboost, cascade indifference curve was explored to determine detection rate and false positive rate of cascade automatically. The experiments showed that the classifier could achieve an overall per-polyp sensitivity of 90% (for polyps' diameter 5 mm and greater), with false positives of 6 per volume on average.
{"title":"Fast and robust polyp detection in CT colonography","authors":"Xin Xiong, Lisheng Xu, Baolin Mao, Xiaozhao Chen, Yan Kang","doi":"10.1109/ICMIPE.2013.6864503","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864503","url":null,"abstract":"A fast and robust colorectal polyp detection framework in CT colonography was proposed. In order to speed the detection of polyp in CT colonography, a cascade-Adaboost framework was employed, and a lot of candidates were rejected quickly in the first stages of the cascade framework. To improve the performance of cascade-Adaboost, cascade indifference curve was explored to determine detection rate and false positive rate of cascade automatically. The experiments showed that the classifier could achieve an overall per-polyp sensitivity of 90% (for polyps' diameter 5 mm and greater), with false positives of 6 per volume on average.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114402880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864493
Jie Zhang, Yun Ge, Ying Chen, Xiangning Chen
Purpose: The positioning accuracy of cancer center is an important factor for radiotherapy effect. The purpose of this study is to investigate the accuracy of patient positioning with Optical Positioning System(OPS) developed by Nanjing University in China, and show its superiority on positioning accuracy, compared with conventional method. Materials and Methods: 20 patients with nasopharyngeal carcinoma picked randomly were investigated. Before treatment, all received patient positioning with conventional method, and OPS's function of tracking and positioning was triggered in the meantime. After patient positioning using conventional method was finished, the three positioning errors along three directions displayed on OPS were observed. If error along any direction was greater than 1.0mm, every step of patient positioning was checked to find error source. Results: In 20 clinical datasets, 15 datasets needed to be checked to find error source. Results showed that the difference of positioning results between conventional method and OPS-guided positioning method came from error source introduced in conventional method basically. And, about 66% positioning error comes from the manual operation error introduced in conventional method. Conclusion: This study indicates that OPS can eliminate the effect of almost manual error source introduced in conventional method basically and improve positioning accuracy of 2-5mm for patients with nasopharyngeal carcinoma.
{"title":"A study on the positioning accuracy of patient positioning based on Optical Positioning System for nasopharyngeal carcinoma: Compared with conventional method","authors":"Jie Zhang, Yun Ge, Ying Chen, Xiangning Chen","doi":"10.1109/ICMIPE.2013.6864493","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864493","url":null,"abstract":"Purpose: The positioning accuracy of cancer center is an important factor for radiotherapy effect. The purpose of this study is to investigate the accuracy of patient positioning with Optical Positioning System(OPS) developed by Nanjing University in China, and show its superiority on positioning accuracy, compared with conventional method. Materials and Methods: 20 patients with nasopharyngeal carcinoma picked randomly were investigated. Before treatment, all received patient positioning with conventional method, and OPS's function of tracking and positioning was triggered in the meantime. After patient positioning using conventional method was finished, the three positioning errors along three directions displayed on OPS were observed. If error along any direction was greater than 1.0mm, every step of patient positioning was checked to find error source. Results: In 20 clinical datasets, 15 datasets needed to be checked to find error source. Results showed that the difference of positioning results between conventional method and OPS-guided positioning method came from error source introduced in conventional method basically. And, about 66% positioning error comes from the manual operation error introduced in conventional method. Conclusion: This study indicates that OPS can eliminate the effect of almost manual error source introduced in conventional method basically and improve positioning accuracy of 2-5mm for patients with nasopharyngeal carcinoma.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116845767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864562
Yue Zhao, Luxuan Qu, Hongbing Hu, Lei Chen
The method on gene classification has been widely studied with the development of gene chip. Machine learning is the best choice to research the issue. But both traditional SVM and ELM cannot fulfill the requirement of high accuracy and short time. Therefore, in this paper, we propose a novel Accuracy Adaptive Extreme Learning Machine (A2-ELM) which can cover the shortage of traditional SVM and ELM in the fact of more dynamic. Firstly, we propose a method of feature selection and overview the property of traditional ELM. Then, an Accuracy of Adaptive ELM (A2-ELM) is developed, which can fulfill the requirement for accurately and rapidly. Finally, we conduct experiments on gene expression data to verify the dynamic and accurate of our proposed accuracy of adaptive ELM in classification gene expression data with experimental settings.
{"title":"An accuracy adaptive breast tumor gene classification method","authors":"Yue Zhao, Luxuan Qu, Hongbing Hu, Lei Chen","doi":"10.1109/ICMIPE.2013.6864562","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864562","url":null,"abstract":"The method on gene classification has been widely studied with the development of gene chip. Machine learning is the best choice to research the issue. But both traditional SVM and ELM cannot fulfill the requirement of high accuracy and short time. Therefore, in this paper, we propose a novel Accuracy Adaptive Extreme Learning Machine (A2-ELM) which can cover the shortage of traditional SVM and ELM in the fact of more dynamic. Firstly, we propose a method of feature selection and overview the property of traditional ELM. Then, an Accuracy of Adaptive ELM (A2-ELM) is developed, which can fulfill the requirement for accurately and rapidly. Finally, we conduct experiments on gene expression data to verify the dynamic and accurate of our proposed accuracy of adaptive ELM in classification gene expression data with experimental settings.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128329718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864547
Weikang Yuan, M. Hamit, A. Kutluk, Chuanbo Yan, Li Li, Jian-Jun Chen, Yan-Ting Hu, Fang Yang
With the rapid development of multimedia technology and network technology and wide application of digital image, more and more attention has been paid for Content-based image retrieval technology. For a long time Xinjiang uygur hospitals and medical institutions accumulated a large amount of underutilized data of uygur medicine. In this paper, the image color histogram feature of botanical and animal drugs of Xinjiang uygur medicine has been extracted. First, the image size has been normalized, and extract the color histogram and analyse color histogram characteristics with statistics method, at last, the classification ability of features is evaluated by Bayes discriminant analysis. Experimental results show that high accuracy for botanical image classification is existed by using color histogram feature. This study would have a certain extent for the content-based medical image retrieval for Xinjiang uygur medicine.
{"title":"Feature extraction and analysis on Xinjiang uygur medicine image by using color histogram","authors":"Weikang Yuan, M. Hamit, A. Kutluk, Chuanbo Yan, Li Li, Jian-Jun Chen, Yan-Ting Hu, Fang Yang","doi":"10.1109/ICMIPE.2013.6864547","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864547","url":null,"abstract":"With the rapid development of multimedia technology and network technology and wide application of digital image, more and more attention has been paid for Content-based image retrieval technology. For a long time Xinjiang uygur hospitals and medical institutions accumulated a large amount of underutilized data of uygur medicine. In this paper, the image color histogram feature of botanical and animal drugs of Xinjiang uygur medicine has been extracted. First, the image size has been normalized, and extract the color histogram and analyse color histogram characteristics with statistics method, at last, the classification ability of features is evaluated by Bayes discriminant analysis. Experimental results show that high accuracy for botanical image classification is existed by using color histogram feature. This study would have a certain extent for the content-based medical image retrieval for Xinjiang uygur medicine.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129633419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864552
Liu Yong, Gao Song, Bao Shanglian, Ma Jingfeng
Objectives: Change the initial value of CV model to achieve the purpose of cell image segmentation fast and accurately. Material and methods: This paper selects slice image of cervical-cancer cells under a microscope as experimental materials. First, the original image is bilateral filtered and then the image is preprocessed using Otsu method to get the rough contour of cytoplasm. Then use Otsu method twice on the cytoplasm to get the rough contour of nucleus. Finally, regard the preprocessed results as the initial value of CV model and evolve the curve with level set method to obtain the final contour. Results: This proposed method costs 17.057s and the iterations are 50, the contour is accurate. Meanwhile, the existed method costs 45.329s and the iterations are 90 and it doesn't iterate to the accurate result. Conclusions: It can obtain accurate cell contour fast regarding the preprocessed result of bilateral filtering and Otsu method as the initial value of CV model.
{"title":"Method for cell image segmentation based on bilateral filtering and CV Model","authors":"Liu Yong, Gao Song, Bao Shanglian, Ma Jingfeng","doi":"10.1109/ICMIPE.2013.6864552","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864552","url":null,"abstract":"Objectives: Change the initial value of CV model to achieve the purpose of cell image segmentation fast and accurately. Material and methods: This paper selects slice image of cervical-cancer cells under a microscope as experimental materials. First, the original image is bilateral filtered and then the image is preprocessed using Otsu method to get the rough contour of cytoplasm. Then use Otsu method twice on the cytoplasm to get the rough contour of nucleus. Finally, regard the preprocessed results as the initial value of CV model and evolve the curve with level set method to obtain the final contour. Results: This proposed method costs 17.057s and the iterations are 50, the contour is accurate. Meanwhile, the existed method costs 45.329s and the iterations are 90 and it doesn't iterate to the accurate result. Conclusions: It can obtain accurate cell contour fast regarding the preprocessed result of bilateral filtering and Otsu method as the initial value of CV model.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113970735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864508
Qingkun Yu, Xiaoning Guan
Radon domain can be filled by the Fourier transforms for projection images in a polar gridding format (radial lines for parallel projections, radon arcs for fan-beam projections). The Radon-based tomographic reconstruction requires regridding a polar radon domain into a rectilinear lattice before inverse Fourier transform. Since the radon domain is irregularly sampled by Fourier-transformed projections, i.e, oversampled around the central regions and undersampled at the peripheral regions, the polar-to-Cartesian coordinate grid conversion involves rebinning for oversampled central region, interpolation for undersampled peripheral region, and extrapolation for extending the peripheral boundary. In this paper, we propose a general data rebinning/interpolation/extrapolation scheme to deal with the radon domain regridding, which is a local convex combination with weights determined by a function of inverse distances. For filling the unavailable entries at peripheral regions, we propose to calculate the corresponding entries in the projection domain, rather than in the radon domain, by interpolations and extrapolations. The interpolation for peripheral region allows us investigate the angular sampling for computed tomography scanning. The extrapolation leads to super-resolution tomographic reconstruction. We find that data interpolation in projection domain may produce better results than in radon domain. This finding may be justified by the fact that the data distribution is more continuous in projection domain than in Fourier domain.
{"title":"Regridding and data interpolation of projection domain and Radon domain for super-resolution tomograpic reconstruction","authors":"Qingkun Yu, Xiaoning Guan","doi":"10.1109/ICMIPE.2013.6864508","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864508","url":null,"abstract":"Radon domain can be filled by the Fourier transforms for projection images in a polar gridding format (radial lines for parallel projections, radon arcs for fan-beam projections). The Radon-based tomographic reconstruction requires regridding a polar radon domain into a rectilinear lattice before inverse Fourier transform. Since the radon domain is irregularly sampled by Fourier-transformed projections, i.e, oversampled around the central regions and undersampled at the peripheral regions, the polar-to-Cartesian coordinate grid conversion involves rebinning for oversampled central region, interpolation for undersampled peripheral region, and extrapolation for extending the peripheral boundary. In this paper, we propose a general data rebinning/interpolation/extrapolation scheme to deal with the radon domain regridding, which is a local convex combination with weights determined by a function of inverse distances. For filling the unavailable entries at peripheral regions, we propose to calculate the corresponding entries in the projection domain, rather than in the radon domain, by interpolations and extrapolations. The interpolation for peripheral region allows us investigate the angular sampling for computed tomography scanning. The extrapolation leads to super-resolution tomographic reconstruction. We find that data interpolation in projection domain may produce better results than in radon domain. This finding may be justified by the fact that the data distribution is more continuous in projection domain than in Fourier domain.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"152 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126199633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864498
Niu Haijun, Fan Fan, Yan Yan, Liang Xiaofei, Wang Li, Li Deyu, Fan Yubo
The early degeneration of articular cartilage (AC) is closely related to the change in glycosaminoglycan (GAG) content. The observation of GAG is therefore an appropriate way for evaluating the degree of AC degeneration and diagnosing osteoarthritis. In this study, the method of contrast-agent enhanced Micro-CT, which has been demonstrated as a potential method for quantitatively analyzing the GAG content, was applied in trypsin digested AC. 102 specimens from fresh bovine femoral condyles were used in this study. 12 specimens were used for exploring the adaptive contrast-agent concentration, incubation time and Micro-CT imaging method. And other 90 were used for GAG degradation experiment. Incubation for 6h with the concentration of 20% contrast-agent Meglumine Diatrizoate (MD) at 37°C was selected as the experiment protocol. Based on this protocol, the relationship between degradation time and the average X-ray attenuation of the samples was obtained, which had a rising tendency within the 50 min degradation (from 1563HU to 1923HU, increased about 23.03%). The average degradation depth of the samples increased with the degradation time (from 0mm (no degradation) to 0.6mm (50 min degradation)). Moreover, the change in the average X-ray attenuation of the samples was linearly and inversely related to their GAG content (r = -0.771, p<; 0.01). To conclude, contrast-agent enhanced Micro-CT can provide useful information about trypsin-induced progressive GAG depletion in AC and can reflect variations of GAG content via X-ray attenuation. The results of this study m provide a basis for quantitatively evaluating the cartilage degradation, and also offer a reference to the early diagnosis of osteoarthritis.
{"title":"Assessment of glycosaminoglycan in progressive trypsin digested cartilage based on contrast-agent enhanced Micro-CT","authors":"Niu Haijun, Fan Fan, Yan Yan, Liang Xiaofei, Wang Li, Li Deyu, Fan Yubo","doi":"10.1109/ICMIPE.2013.6864498","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864498","url":null,"abstract":"The early degeneration of articular cartilage (AC) is closely related to the change in glycosaminoglycan (GAG) content. The observation of GAG is therefore an appropriate way for evaluating the degree of AC degeneration and diagnosing osteoarthritis. In this study, the method of contrast-agent enhanced Micro-CT, which has been demonstrated as a potential method for quantitatively analyzing the GAG content, was applied in trypsin digested AC. 102 specimens from fresh bovine femoral condyles were used in this study. 12 specimens were used for exploring the adaptive contrast-agent concentration, incubation time and Micro-CT imaging method. And other 90 were used for GAG degradation experiment. Incubation for 6h with the concentration of 20% contrast-agent Meglumine Diatrizoate (MD) at 37°C was selected as the experiment protocol. Based on this protocol, the relationship between degradation time and the average X-ray attenuation of the samples was obtained, which had a rising tendency within the 50 min degradation (from 1563HU to 1923HU, increased about 23.03%). The average degradation depth of the samples increased with the degradation time (from 0mm (no degradation) to 0.6mm (50 min degradation)). Moreover, the change in the average X-ray attenuation of the samples was linearly and inversely related to their GAG content (r = -0.771, p<; 0.01). To conclude, contrast-agent enhanced Micro-CT can provide useful information about trypsin-induced progressive GAG depletion in AC and can reflect variations of GAG content via X-ray attenuation. The results of this study m provide a basis for quantitatively evaluating the cartilage degradation, and also offer a reference to the early diagnosis of osteoarthritis.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134348849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-10-01DOI: 10.1109/ICMIPE.2013.6864515
Defeng Li, Xi-zhen Wang, Qin-yan Xu, Yingying Zhang, Xihe Su, Peng Dong, Yue Guan, Guang-hui Chang, Yan-ming Ge, W. Fu
Purpose: Using 1.5 T proton magnetic resonance spectroscopy (1H-MRS) with high resolution 23mm special coil in vivo to monitor the metabolic changes of the nerves in the striatum of Parkinson's disease (PD) rat before and after mesenchymal stem cells (MSCs) transplantation, in result to explore the value of 1.5T clinical MR spectral analysis in evaluating the therapeutic effects of MSCs transplantation. Materials and methods: Thirty cases of normal rats were treated as Parkinson's disease models with unilateral symptom by taking 6-hydroxydopamine (6-OHDA) to enforce unilateral (right) lesion, and 26 cases were successful. 1H-MRS was performed in the vivo rats using Philips 1.5T two gradients clinical MR machine with high resolution 23mm special coil on the three weeks point after lesion establishment, and on the three, six and nine weeks points after MSCs and serum-free medium injected respectively. The changes of NAA/Cr, Cho/Cr ratio in the bilateral striatum were analyzed. Meanwhile, the rat behavior was detected. The expression characteristics of tyrosine hydroxylase (TH) were checked in substantia nigra and the migration of the transplanted cells were observed using immunocytochemical and immunofluorescent methods. Results: At 6 weeks and 9 weeks points after MSCs transplanted, NAA/Cr ratios increased gradually in striatum of the damage side (right) comparing with the control group, and significant difference was showed between them (P<;0.05). At nine-week point after MSCs transplantation, NAA/Cr ratio was higher than that at six weeks point (P<;0.05). Cho/Cr ratios on the damaged side in MSCs transplantation group was lower than control group at 3, 6, 9 weeks points respectively (P<;0.05), but no significant difference was shown among 3, 6, 9 weeks points (P> 0.05). NAA/Cr ratios and Cho/Cr ratios in damaged side of MSCs transplantation group decreased comparing with their opposite parts respectively (P<;0.05). The number of rat's full rotations at 6 weeks and 9 weeks points after MSCs transplanted gradually showed a significant reduction compared with other rats (P<;0.05). The survival rates of TH-positive cells in the damage side striatum among two injected groups were no significant difference (P>0.05). However, by immunofluorescence staining, a part of BrdU labeled MSCs showed GFAP-positive, which conduced to the function of nerves repairing. Conclusion: As a non-invasive examination method in vivo, 1.5T 1H-MRS can be used to monitor the metabolic changes dynamically in the striatum of PD rat model after MSCs transplantation, that was able to evaluate the therapeutic effects efficiently.
{"title":"Evaluation of the therapeutic effects of mesenchymal stem cell transplantation in rat model of Parkinson's disease using 1.5T MRS","authors":"Defeng Li, Xi-zhen Wang, Qin-yan Xu, Yingying Zhang, Xihe Su, Peng Dong, Yue Guan, Guang-hui Chang, Yan-ming Ge, W. Fu","doi":"10.1109/ICMIPE.2013.6864515","DOIUrl":"https://doi.org/10.1109/ICMIPE.2013.6864515","url":null,"abstract":"Purpose: Using 1.5 T proton magnetic resonance spectroscopy (1H-MRS) with high resolution 23mm special coil in vivo to monitor the metabolic changes of the nerves in the striatum of Parkinson's disease (PD) rat before and after mesenchymal stem cells (MSCs) transplantation, in result to explore the value of 1.5T clinical MR spectral analysis in evaluating the therapeutic effects of MSCs transplantation. Materials and methods: Thirty cases of normal rats were treated as Parkinson's disease models with unilateral symptom by taking 6-hydroxydopamine (6-OHDA) to enforce unilateral (right) lesion, and 26 cases were successful. 1H-MRS was performed in the vivo rats using Philips 1.5T two gradients clinical MR machine with high resolution 23mm special coil on the three weeks point after lesion establishment, and on the three, six and nine weeks points after MSCs and serum-free medium injected respectively. The changes of NAA/Cr, Cho/Cr ratio in the bilateral striatum were analyzed. Meanwhile, the rat behavior was detected. The expression characteristics of tyrosine hydroxylase (TH) were checked in substantia nigra and the migration of the transplanted cells were observed using immunocytochemical and immunofluorescent methods. Results: At 6 weeks and 9 weeks points after MSCs transplanted, NAA/Cr ratios increased gradually in striatum of the damage side (right) comparing with the control group, and significant difference was showed between them (P<;0.05). At nine-week point after MSCs transplantation, NAA/Cr ratio was higher than that at six weeks point (P<;0.05). Cho/Cr ratios on the damaged side in MSCs transplantation group was lower than control group at 3, 6, 9 weeks points respectively (P<;0.05), but no significant difference was shown among 3, 6, 9 weeks points (P> 0.05). NAA/Cr ratios and Cho/Cr ratios in damaged side of MSCs transplantation group decreased comparing with their opposite parts respectively (P<;0.05). The number of rat's full rotations at 6 weeks and 9 weeks points after MSCs transplanted gradually showed a significant reduction compared with other rats (P<;0.05). The survival rates of TH-positive cells in the damage side striatum among two injected groups were no significant difference (P>0.05). However, by immunofluorescence staining, a part of BrdU labeled MSCs showed GFAP-positive, which conduced to the function of nerves repairing. Conclusion: As a non-invasive examination method in vivo, 1.5T 1H-MRS can be used to monitor the metabolic changes dynamically in the striatum of PD rat model after MSCs transplantation, that was able to evaluate the therapeutic effects efficiently.","PeriodicalId":135461,"journal":{"name":"2013 IEEE International Conference on Medical Imaging Physics and Engineering","volume":"483 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132687010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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