Shaqayeq Ramezanzade, Tudor Laurentiu Dascalu, Azam Bakhshandeh, Sergio E Uribe, Bulat Ibragimov, Lars Bjørndal
Aim: This study evaluated the effect of a short, personalised training session on student performance in using an artificial intelligence (AI)-based platform for pulp exposure prediction before caries excavation and determined the required sample size for a further randomised controlled trial (RCT).
Methodology: Undergraduate dental students were randomly assigned to the experimental (training) group and the control (no training) group. The training group received a 1-h training session before undertaking the experiment, focusing on the uses, applications, and drawbacks of AI and carious lesion penetration depth. The theoretical presentation was followed by practical exercises and a quiz to check learning progress. Later, participants in both groups completed an experimental task involving 292 cases. They were asked to predict pulp exposure using an AI-based website. Sample size calculations determined the required sample size, with 80% power and an alpha of 5%.
Results: 18 participants were enrolled (9 in each group). The agreement between participants' decisions and AI predictions regarding the presence or absence of pulp exposure (agreeableness with AI) was higher in the training group compared to the control group (0.83 vs. 0.76). The training group had a slightly higher mean F1-score (0.63 vs. 0.62), accuracy (0.69 vs. 0.68), and sensitivity (0.63 vs. 0.59) than the control group. Based on the sample size calculation, at least 31 participants per group are needed for the future RCT.
Conclusions: The results support further investigation of customised training sessions prior to using an AI-based platform to assess their impact on dental students' agreement with AI predictions.
目的：本研究评估了短期个性化培训课程对学生在龋挖掘前使用基于人工智能（AI）的牙髓暴露预测平台表现的影响，并确定了进一步随机对照试验（RCT）所需的样本量。方法：将牙科本科学生随机分为实验组（训练组）和对照组（未训练组）。实验组在实验前进行1小时的培训，重点了解人工智能和龋齿穿透深度的用途、应用和不足。理论陈述之后是实践练习和测试，以检查学习进度。随后，两组参与者都完成了一项涉及292个案例的实验任务。他们被要求使用一个基于人工智能的网站来预测牙髓暴露。样本量计算确定所需的样本量，功率为80%，alpha为5%。结果：共入组18例（每组9例）。与对照组相比，训练组参与者的决定与人工智能预测之间关于是否存在牙髓暴露（与人工智能的亲和性）的一致性更高（0.83对0.76）。训练组的平均f1得分（0.63 vs. 0.62）、准确率（0.69 vs. 0.68）和灵敏度（0.63 vs. 0.59）略高于对照组。根据样本量计算，未来的RCT每组至少需要31名参与者。结论：结果支持在使用基于人工智能的平台之前进一步调查定制培训课程，以评估其对牙科学生同意人工智能预测的影响。试验注册：ClinicalTrial.gov标识符：NCT05912361。
{"title":"The Impact of Training Dental Students to Use an Artificial Intelligence-Based Platform for Pulp Exposure Prediction Prior to Deep Caries Excavation: A Proof-of-Concept Randomised Controlled Trial.","authors":"Shaqayeq Ramezanzade, Tudor Laurentiu Dascalu, Azam Bakhshandeh, Sergio E Uribe, Bulat Ibragimov, Lars Bjørndal","doi":"10.1111/iej.70046","DOIUrl":"https://doi.org/10.1111/iej.70046","url":null,"abstract":"<p><strong>Aim: </strong>This study evaluated the effect of a short, personalised training session on student performance in using an artificial intelligence (AI)-based platform for pulp exposure prediction before caries excavation and determined the required sample size for a further randomised controlled trial (RCT).</p><p><strong>Methodology: </strong>Undergraduate dental students were randomly assigned to the experimental (training) group and the control (no training) group. The training group received a 1-h training session before undertaking the experiment, focusing on the uses, applications, and drawbacks of AI and carious lesion penetration depth. The theoretical presentation was followed by practical exercises and a quiz to check learning progress. Later, participants in both groups completed an experimental task involving 292 cases. They were asked to predict pulp exposure using an AI-based website. Sample size calculations determined the required sample size, with 80% power and an alpha of 5%.</p><p><strong>Results: </strong>18 participants were enrolled (9 in each group). The agreement between participants' decisions and AI predictions regarding the presence or absence of pulp exposure (agreeableness with AI) was higher in the training group compared to the control group (0.83 vs. 0.76). The training group had a slightly higher mean F1-score (0.63 vs. 0.62), accuracy (0.69 vs. 0.68), and sensitivity (0.63 vs. 0.59) than the control group. Based on the sample size calculation, at least 31 participants per group are needed for the future RCT.</p><p><strong>Conclusions: </strong>The results support further investigation of customised training sessions prior to using an AI-based platform to assess their impact on dental students' agreement with AI predictions.</p><p><strong>Trial registration: </strong>ClinicalTrial.gov identifier: NCT05912361.</p>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":" ","pages":""},"PeriodicalIF":7.1,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145274492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To evaluate the accuracy of guided access cavity preparation in calcified canals using a robotic system, compared to static and dynamic guided methods.
Methodology
Three 3D-printed mandibular models presenting with pulp canal obliteration were allocated into three groups for robot, static and dynamic-guided access cavity preparations. The primary outcomes measured were the coronal deviation, apical deviation and angular deviation between the planned and actual paths of access cavity preparation. Additionally, all access cavities were confirmed to determine whether the canal was located. The data were analysed using the one-way analysis of variance (ANOVA), Tukey's post hoc test and multilevel linear regression models. The significance level was set at 0.05.
Results
All canals (n = 12) in the robot-guided and static-guided groups were located. In the dynamic guided group, a total of 10 out of 12 canals were found, with 2 resulting in root perforations. The accuracy of access cavity preparation using a robotic system showed no significant difference compared to the static-guided group (p > 0.05). However, the apical and angular deviation results were significantly lower in the robot-guided group than the dynamic-guided group (p < 0.05).
Conclusions
The robotic system exhibited comparable accuracy to the static guide, whereas it significantly outperformed dynamic navigation in terms of guided access cavity preparation for calcified root canals. However, further clinical studies are needed to confirm these preliminary findings.
{"title":"Accuracy of Robot-Guided Access Cavity Preparation in Managing Calcified Canals: An In Vitro Study","authors":"Yuchen Liu, Chen Liu, Shizhu Bai, Yimin Zhao","doi":"10.1111/iej.70049","DOIUrl":"10.1111/iej.70049","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To evaluate the accuracy of guided access cavity preparation in calcified canals using a robotic system, compared to static and dynamic guided methods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>Three 3D-printed mandibular models presenting with pulp canal obliteration were allocated into three groups for robot, static and dynamic-guided access cavity preparations. The primary outcomes measured were the coronal deviation, apical deviation and angular deviation between the planned and actual paths of access cavity preparation. Additionally, all access cavities were confirmed to determine whether the canal was located. The data were analysed using the one-way analysis of variance (ANOVA), Tukey's post hoc test and multilevel linear regression models. The significance level was set at 0.05.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>All canals (<i>n</i> = 12) in the robot-guided and static-guided groups were located. In the dynamic guided group, a total of 10 out of 12 canals were found, with 2 resulting in root perforations. The accuracy of access cavity preparation using a robotic system showed no significant difference compared to the static-guided group (<i>p</i> > 0.05). However, the apical and angular deviation results were significantly lower in the robot-guided group than the dynamic-guided group (<i>p</i> < 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The robotic system exhibited comparable accuracy to the static guide, whereas it significantly outperformed dynamic navigation in terms of guided access cavity preparation for calcified root canals. However, further clinical studies are needed to confirm these preliminary findings.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"59 2","pages":"262-270"},"PeriodicalIF":7.1,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145258120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xavier Roig-Soriano, Luis María Delgado, Firas Elmsmari, Fernando Duran-Sindreu, Miriam Teulé, Marta Espina, Gerard Esteruelas, Maria Luisa García, José Antonio González Sánchez, Elena Sánchez-López
Aim
To characterise and evaluate mucoadhesive strength and antibacterial properties of a nanotechnological formulation for endodontic disinfection based on biodegradable nanoparticles dispersed in a thermosensitive gel containing calcium hydroxide (Ca(OH)2-NPs-gel).
Methodology
Morphology of Ca(OH)2-NPs-gel was studied using transmission electron microscopy. Moreover, Ca(OH)2-NPs-gel was sterilised using gamma irradiation (25 kGy), and the stability after the sterilisation process was studied by measuring Ca(OH)2-NPs-gel average size, polydispersity index, zeta potential and encapsulation efficiency. To assess the ex vivo mucoadhesive strength, extracted single-rooted human teeth were used to measure the force necessary to separate the formulation from the teeth. In addition, the short-time stability of Ca(OH)2-NPs-gel was evaluated monthly, analysing entrapment efficacy, backscattering and transmittance of Ca(OH)2-NPs-gel stored at different temperatures (4°C, 25°C and 37°C). Furthermore, the antibacterial analysis of Ca(OH)2-NPs-gel was performed against Enterococcus faecalis inoculated in extracted human single-root teeth and evaluated by confocal and scanning electron microscopy. Finally, the metabolic activity of bacteria was studied through a resazurin assay to evaluate bacterial survival after treatment.
Results
Ca(OH)2-NPs-gel owned a round shape and a smooth surface without particle aggregation. Sterilisation did not induce an alteration in Ca(OH)2-NPs-gel physicochemical properties and Ca(OH)2-NPs-gel presented a high adhesion strength. In addition, 4°C was the best temperature to store Ca(OH)2-NPs-gel. Regarding the antibacterial therapeutic efficacy, Ca(OH)2-NPs-gel possesses suitable antibacterial properties, indicating that it efficiently reduces bacterial biofilms.
Conclusion
Calcium hydroxide-loaded PLGA nanoparticles dispersed in a thermosensitive gel have been developed, optimised and characterised, obtaining excellent antibacterial properties and achieving bacterial disinfection levels similar to those of commercial formulations.
{"title":"A Thermosensitive Gel Containing Biodegradable Nanoparticles Carrying Calcium Hydroxide as Antibacterial Intracanal Therapy","authors":"Xavier Roig-Soriano, Luis María Delgado, Firas Elmsmari, Fernando Duran-Sindreu, Miriam Teulé, Marta Espina, Gerard Esteruelas, Maria Luisa García, José Antonio González Sánchez, Elena Sánchez-López","doi":"10.1111/iej.70032","DOIUrl":"10.1111/iej.70032","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To characterise and evaluate mucoadhesive strength and antibacterial properties of a nanotechnological formulation for endodontic disinfection based on biodegradable nanoparticles dispersed in a thermosensitive gel containing calcium hydroxide (Ca(OH)<sub>2</sub>-NPs-gel).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>Morphology of Ca(OH)<sub>2</sub>-NPs-gel was studied using transmission electron microscopy. Moreover, Ca(OH)<sub>2</sub>-NPs-gel was sterilised using gamma irradiation (25 kGy), and the stability after the sterilisation process was studied by measuring Ca(OH)<sub>2</sub>-NPs-gel average size, polydispersity index, zeta potential and encapsulation efficiency. To assess the ex vivo mucoadhesive strength, extracted single-rooted human teeth were used to measure the force necessary to separate the formulation from the teeth. In addition, the short-time stability of Ca(OH)<sub>2</sub>-NPs-gel was evaluated monthly, analysing entrapment efficacy, backscattering and transmittance of Ca(OH)<sub>2</sub>-NPs-gel stored at different temperatures (4°C, 25°C and 37°C). Furthermore, the antibacterial analysis of Ca(OH)<sub>2</sub>-NPs-gel was performed against <i>Enterococcus faecalis</i> inoculated in extracted human single-root teeth and evaluated by confocal and scanning electron microscopy. Finally, the metabolic activity of bacteria was studied through a resazurin assay to evaluate bacterial survival after treatment.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ca(OH)<sub>2</sub>-NPs-gel owned a round shape and a smooth surface without particle aggregation. Sterilisation did not induce an alteration in Ca(OH)<sub>2</sub>-NPs-gel physicochemical properties and Ca(OH)<sub>2</sub>-NPs-gel presented a high adhesion strength. In addition, 4°C was the best temperature to store Ca(OH)<sub>2</sub>-NPs-gel. Regarding the antibacterial therapeutic efficacy, Ca(OH)<sub>2</sub>-NPs-gel possesses suitable antibacterial properties, indicating that it efficiently reduces bacterial biofilms.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Calcium hydroxide-loaded PLGA nanoparticles dispersed in a thermosensitive gel have been developed, optimised and characterised, obtaining excellent antibacterial properties and achieving bacterial disinfection levels similar to those of commercial formulations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"59 1","pages":"163-176"},"PeriodicalIF":7.1,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nour Ghazi, Har-amrit Singh, Edward Longbottom, Jeremy Hayes, Damian Farnell, Arindam Dutta
<div> <section> <h3> Aim</h3> <p>To assess the complexity of root canal treatments allocated to Postgraduate Endodontology trainees at Cardiff University Dental Hospital (CUDH) using the English Clinical Standards for Restorative Dentistry (ECS) in comparison with the Dental Practicality Index (DPI), the EndoApp (EA), and the Endodontic Complexity Assessment Tool (E-CAT).</p> </section> <section> <h3> Material and Methods</h3> <p>Two-hundred-and-one case records were evaluated by two calibrated examiners using each complexity assessment system. Inter-examiner and intra-examiner variability was calculated using Cohen's kappa coefficient. Statistical analyses compared the scores obtained for the same case using the different systems.</p> </section> <section> <h3> Results</h3> <p>Most cases were assigned level 3 complexity using ECS, EA and E-CAT (82%, 92% and 74.1%, respectively), and scores of 3–5 (78.6%) using DPI. EA consistently assigned higher complexity scores compared with ECS and E-CAT. E-CAT assigned lower complexity scores compared with ECS. A statistically significant moderate–substantial level of agreement was demonstrated between E-CAT and ECS (weighted kappa = 0.647 [95% CI: 0.517 to 0.776], <i>p</i> < 0.001). A statistically significant fair level of agreement was demonstrated between EA and ECS (weighted kappa = 0.290 [95% CI: 0.113 to 0.466], <i>p</i> < 0.001) and EA and E-CAT (weighted kappa: 0.385 [95% CI: 0.226 to 0.544], <i>p</i> < 0.001). A statistically significant weak positive correlation was found between DPI and ECS [Spearman's correlation coefficient (<i>r</i><sub>s</sub>) = 0.202, <i>p</i> = 0.004], DPI and EA (<i>r</i><sub>s</sub> = 0.344, <i>p</i> < 0.001), and DPI and E-CAT (<i>r</i><sub>s</sub> = 0.364, <i>p</i> < 0.001). The most common cause of increase in complexity scores was ‘canal negotiability’ for ECS (47%) and the ‘endodontic treatment need’ for DPI (84.1%). The unknown algorithm used by EA and E-CAT prevented the identification of specific factors that contributed to the endodontic treatment complexity.</p> </section> <section> <h3> Conclusion</h3> <p>The majority of cases treated at CUDH were of high complexity. E-CAT assigned slightly lower complexity scores compared with ECS and EA, potentially due to its detailed assessment of factors. A weak positive correlation was found between the complexity grading systems. DPI's broader assessment justifies a cut-off score of 3 for specialist referral due to the increased agreement with ECS, E-CAT and EA at this threshold.</p> </sectio
目的：通过与牙科实用性指数（DPI）、牙髓应用程序（EA）和牙髓复杂性评估工具（E-CAT）比较，评估卡迪夫大学牙科医院（CUDH）牙髓学研究生使用英国临床标准（ECS）进行根管治疗的复杂性。材料和方法：由两名经过校准的审查员使用每种复杂性评估系统对221份病例记录进行评估。使用Cohen's kappa系数计算考官之间和考官内部的变异性。统计分析比较了使用不同系统对同一病例获得的分数。结果：大多数病例的ECS、EA和E-CAT评分为3级（分别为82%、92%和74.1%），DPI评分为3-5级（78.6%）。与ECS和E-CAT相比，EA始终分配更高的复杂性分数。与ECS相比，E-CAT的复杂性评分较低。E-CAT与ECS（加权kappa = 0.647 [95% CI: 0.517 ~ 0.776], p s） = 0.202, p = 0.004)、DPI与EA （rs = 0.344, p s = 0.364, p）的一致性具有统计学意义。与ECS和EA相比，E-CAT的复杂性评分略低，这可能是由于其对因素的详细评估。复杂度分级系统之间存在弱正相关关系。由于与ECS， E-CAT和EA在这一阈值上的一致性增加，DPI更广泛的评估证明了专家转诊的截止分数为3分。
{"title":"Assessment of Case Complexity of Root Canal Treatments Using Contemporary Complexity Grading Systems: A Clinical Service Evaluation","authors":"Nour Ghazi, Har-amrit Singh, Edward Longbottom, Jeremy Hayes, Damian Farnell, Arindam Dutta","doi":"10.1111/iej.70039","DOIUrl":"10.1111/iej.70039","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To assess the complexity of root canal treatments allocated to Postgraduate Endodontology trainees at Cardiff University Dental Hospital (CUDH) using the English Clinical Standards for Restorative Dentistry (ECS) in comparison with the Dental Practicality Index (DPI), the EndoApp (EA), and the Endodontic Complexity Assessment Tool (E-CAT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>Two-hundred-and-one case records were evaluated by two calibrated examiners using each complexity assessment system. Inter-examiner and intra-examiner variability was calculated using Cohen's kappa coefficient. Statistical analyses compared the scores obtained for the same case using the different systems.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Most cases were assigned level 3 complexity using ECS, EA and E-CAT (82%, 92% and 74.1%, respectively), and scores of 3–5 (78.6%) using DPI. EA consistently assigned higher complexity scores compared with ECS and E-CAT. E-CAT assigned lower complexity scores compared with ECS. A statistically significant moderate–substantial level of agreement was demonstrated between E-CAT and ECS (weighted kappa = 0.647 [95% CI: 0.517 to 0.776], <i>p</i> < 0.001). A statistically significant fair level of agreement was demonstrated between EA and ECS (weighted kappa = 0.290 [95% CI: 0.113 to 0.466], <i>p</i> < 0.001) and EA and E-CAT (weighted kappa: 0.385 [95% CI: 0.226 to 0.544], <i>p</i> < 0.001). A statistically significant weak positive correlation was found between DPI and ECS [Spearman's correlation coefficient (<i>r</i><sub>s</sub>) = 0.202, <i>p</i> = 0.004], DPI and EA (<i>r</i><sub>s</sub> = 0.344, <i>p</i> < 0.001), and DPI and E-CAT (<i>r</i><sub>s</sub> = 0.364, <i>p</i> < 0.001). The most common cause of increase in complexity scores was ‘canal negotiability’ for ECS (47%) and the ‘endodontic treatment need’ for DPI (84.1%). The unknown algorithm used by EA and E-CAT prevented the identification of specific factors that contributed to the endodontic treatment complexity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The majority of cases treated at CUDH were of high complexity. E-CAT assigned slightly lower complexity scores compared with ECS and EA, potentially due to its detailed assessment of factors. A weak positive correlation was found between the complexity grading systems. DPI's broader assessment justifies a cut-off score of 3 for specialist referral due to the increased agreement with ECS, E-CAT and EA at this threshold.</p>\u0000 </sectio","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"59 1","pages":"47-56"},"PeriodicalIF":7.1,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.70039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peiling Hu, Yingqing Chen, Ping Long, Anni Zhang, Xiaorong Lan, Yuanpei He, Guangwen Li, Shiting Li
<div> <section> <h3> Aim</h3> <p>Under certain conditions, infection-induced inflammation may activate reparative processes to form a hard tissue barrier against microbial invasion. Autophagy participates in odontogenic differentiation during inflammation in vitro, but its role in pulp repair remains unclear. This study investigated how autophagy regulated odontogenic differentiation within the inflammatory microenvironment, emphasising the regulatory role of connexin43 (Cx43) on autophagy.</p> </section> <section> <h3> Methodology</h3> <p>Autophagy activation was detected in inflamed pulpal tissues using immunofluorescence (IF). Human dental pulp cells (hDPCs) were stimulated with 0.1/5 μg/mL lipopolysaccharide (LPS). Autophagy dynamics, including autophagic flux, were analysed through western blotting (WB), transmission electron microscopy (TEM), and mRFP-GFP-LC3 plasmid transfection. Odontogenic differentiation and mineralisation were assessed using molecular assays and Alizarin Red staining. Pharmacological inhibitors or activators were applied to determine autophagy's role. Cx43 knockdown in hDPCs and a dentine injury model in Cx43 cKO mice were used to validate their effects on autophagy. Results were analysed by two-way ANOVA.</p> </section> <section> <h3> Results</h3> <p>Autophagy-related proteins were predominantly localised in the odontoblast layer. As dental pulp infection advanced, concurrent upregulation of LC3 and p62 levels was observed, indicating that autophagy activation occurs in the pulp alongside potential impairment of autophagic flux. 0.1 μg/mL LPS promoted autophagic flux, thereby facilitating odontogenic differentiation and mineralisation, whereas autophagy inhibition attenuated these effects. Conversely, 5 μg/mL LPS induced autophagosome accumulation but blocked autophagic flux, suppressing odontogenic differentiation and mineralisation; however, restoring autophagic flux reversed this inhibition. These data suggested that maintenance of autophagic flux integrity is essential for sustaining odontogenic differentiation capacity under inflammatory stress. Cx43 knockdown under high-inflammatory conditions rescued autophagic flux and improved differentiation. Similarly, in the dentine injury model of cKO mice, Cx43 deletion attenuated p62 expression while upregulating DSPP expression, accompanied by enhanced tertiary dentine formation beneath the injury site, indicating that blockade of Cx43 promotes autophagic flux to improve pulp repair.</p> </section> <section> <h3> Conclusion</h3> <p>Autophagy regulates both inflammatory r
{"title":"From Cells to Animals: Connexin43 Suppression Enhances Autophagic Flux to Restore Odontogenesis in Inflamed Dental Pulp","authors":"Peiling Hu, Yingqing Chen, Ping Long, Anni Zhang, Xiaorong Lan, Yuanpei He, Guangwen Li, Shiting Li","doi":"10.1111/iej.70044","DOIUrl":"10.1111/iej.70044","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Under certain conditions, infection-induced inflammation may activate reparative processes to form a hard tissue barrier against microbial invasion. Autophagy participates in odontogenic differentiation during inflammation in vitro, but its role in pulp repair remains unclear. This study investigated how autophagy regulated odontogenic differentiation within the inflammatory microenvironment, emphasising the regulatory role of connexin43 (Cx43) on autophagy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>Autophagy activation was detected in inflamed pulpal tissues using immunofluorescence (IF). Human dental pulp cells (hDPCs) were stimulated with 0.1/5 μg/mL lipopolysaccharide (LPS). Autophagy dynamics, including autophagic flux, were analysed through western blotting (WB), transmission electron microscopy (TEM), and mRFP-GFP-LC3 plasmid transfection. Odontogenic differentiation and mineralisation were assessed using molecular assays and Alizarin Red staining. Pharmacological inhibitors or activators were applied to determine autophagy's role. Cx43 knockdown in hDPCs and a dentine injury model in Cx43 cKO mice were used to validate their effects on autophagy. Results were analysed by two-way ANOVA.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Autophagy-related proteins were predominantly localised in the odontoblast layer. As dental pulp infection advanced, concurrent upregulation of LC3 and p62 levels was observed, indicating that autophagy activation occurs in the pulp alongside potential impairment of autophagic flux. 0.1 μg/mL LPS promoted autophagic flux, thereby facilitating odontogenic differentiation and mineralisation, whereas autophagy inhibition attenuated these effects. Conversely, 5 μg/mL LPS induced autophagosome accumulation but blocked autophagic flux, suppressing odontogenic differentiation and mineralisation; however, restoring autophagic flux reversed this inhibition. These data suggested that maintenance of autophagic flux integrity is essential for sustaining odontogenic differentiation capacity under inflammatory stress. Cx43 knockdown under high-inflammatory conditions rescued autophagic flux and improved differentiation. Similarly, in the dentine injury model of cKO mice, Cx43 deletion attenuated p62 expression while upregulating DSPP expression, accompanied by enhanced tertiary dentine formation beneath the injury site, indicating that blockade of Cx43 promotes autophagic flux to improve pulp repair.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Autophagy regulates both inflammatory r","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"59 1","pages":"134-152"},"PeriodicalIF":7.1,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<div> <section> <h3> Background</h3> <p>Dental pulp sustains oral health through dentine formation, sensation, nutrition and defence. Beyond these classical roles, pulpal nerves have emerged as key coordinators of immune responses and tissue repair. While traditionally viewed as limited to sensory and vascular regulation, recent advances reveal that the nervous system interacts with immune mechanisms to manage inflammation and facilitate regeneration following injury or infection.</p> </section> <section> <h3> Objectives</h3> <p>To synthesise evidence on the anatomy, distribution and functional diversity of dental pulp innervation, emphasising how nerves regulate innate/adaptive immunity and orchestrate pulp tissue repair and regeneration.</p> </section> <section> <h3> Method</h3> <p>The review included studies up to the search period, utilising PubMed and Google Scholar. Search terms covered nervous-system concepts (“nerves”, “neuropeptides”, “nervefibres”, “Schwann cells”) and immune/repair concepts (“immune response”, “inflammation”, “immune cells”, “fibroblasts”, “odontoblasts”, “dental pulpstem cells”, “dentine regeneration”, “odontogenic differentiation”). Key termswere combined with Boolean operators: [nervous-system terms] AND [immune/repairterms] AND [dental], to capture studies on the role of dental nerves in pulpimmune defence and repair.</p> </section> <section> <h3> Results</h3> <p>In innate immunity, dental nerves recognise pathogens, undergo sprouting in inflamed pulp, mediate neurogenic inflammation and engage in dynamic crosstalk with immune cells. In adaptive immunity, they influence antigen presentation and regulate T-cell activation through neuropeptides, highlighting their central role in immune modulation. Furthermore, dental nerves are indispensable for tissue repair, facilitating key processes such as progenitor cell recruitment, proliferation, differentiation and reparative dentine formation. Schwann cells and sensory and sympathetic innervation play distinct yet interconnected roles throughout these stages.</p> </section> <section> <h3> Conclusion</h3> <p>The neuro-immune-endodontic axis is central to the pulp's ability to defend and repair itself, offering insights into innovative therapeutic strategies for managing dental pulp diseases. By integrating neural regulation into endodontic therapies, we can develop more effective treatments that leverage the complex interplay between the nervous and immune systems, enhancing
{"title":"Unveiling the Vital Role of Dental Nerves in Dental Pulp Immune Defence and Repair","authors":"Wenji Luo, Mingmei Yang, Chi Zhan, Zongshan Shen, Zhi Song, Zhengmei Lin, Lingling Chen","doi":"10.1111/iej.70040","DOIUrl":"10.1111/iej.70040","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Dental pulp sustains oral health through dentine formation, sensation, nutrition and defence. Beyond these classical roles, pulpal nerves have emerged as key coordinators of immune responses and tissue repair. While traditionally viewed as limited to sensory and vascular regulation, recent advances reveal that the nervous system interacts with immune mechanisms to manage inflammation and facilitate regeneration following injury or infection.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To synthesise evidence on the anatomy, distribution and functional diversity of dental pulp innervation, emphasising how nerves regulate innate/adaptive immunity and orchestrate pulp tissue repair and regeneration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Method</h3>\u0000 \u0000 <p>The review included studies up to the search period, utilising PubMed and Google Scholar. Search terms covered nervous-system concepts (“nerves”, “neuropeptides”, “nervefibres”, “Schwann cells”) and immune/repair concepts (“immune response”, “inflammation”, “immune cells”, “fibroblasts”, “odontoblasts”, “dental pulpstem cells”, “dentine regeneration”, “odontogenic differentiation”). Key termswere combined with Boolean operators: [nervous-system terms] AND [immune/repairterms] AND [dental], to capture studies on the role of dental nerves in pulpimmune defence and repair.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In innate immunity, dental nerves recognise pathogens, undergo sprouting in inflamed pulp, mediate neurogenic inflammation and engage in dynamic crosstalk with immune cells. In adaptive immunity, they influence antigen presentation and regulate T-cell activation through neuropeptides, highlighting their central role in immune modulation. Furthermore, dental nerves are indispensable for tissue repair, facilitating key processes such as progenitor cell recruitment, proliferation, differentiation and reparative dentine formation. Schwann cells and sensory and sympathetic innervation play distinct yet interconnected roles throughout these stages.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The neuro-immune-endodontic axis is central to the pulp's ability to defend and repair itself, offering insights into innovative therapeutic strategies for managing dental pulp diseases. By integrating neural regulation into endodontic therapies, we can develop more effective treatments that leverage the complex interplay between the nervous and immune systems, enhancing ","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"59 1","pages":"2-18"},"PeriodicalIF":7.1,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joséphine Brodén, Maria Pigg, Niklas Vareman, Helena Fransson
Aim: In uncertain clinical situations, such as assessing the risk of exacerbation of apical periodontitis in root-filled teeth, undergraduate students can benefit from being trained to reflect. This study aimed to explore how students specifically reflected on clinical experiences in relation to uncertainty when assessing a case involving a root-filled tooth with apical periodontitis.
Methodology: Short reflections were written by 52 dental students at Malmö University in 2021. All students were asked to reflect on the risk for exacerbation of apical periodontitis in a case with a root-filled tooth with a diffuse widening of the periodontal ligament space. The reflections were written according to prompts developed to stimulate reflection. For each student, the reflections were analysed by a qualitative method, Systematic Text Condensation (STC).
Results: The analysis of the qualitative data resulted in the formulation of two themes about experience and lack of experience: Theme 1 'The meaning of clinical experience' and Theme 2 'Differences and similarities'. The themes were subdivided into nine subgroups and described the relationship between experience and certainty.
Conclusions: The students believed that certainty comes with experience even when there is a lack of scientific evidence.
{"title":"Uncertainty in Relation to Clinical Experience-A Qualitative Study on Dental Students' Reflections on Risk Assessment of Root Filled Teeth.","authors":"Joséphine Brodén, Maria Pigg, Niklas Vareman, Helena Fransson","doi":"10.1111/iej.70047","DOIUrl":"https://doi.org/10.1111/iej.70047","url":null,"abstract":"<p><strong>Aim: </strong>In uncertain clinical situations, such as assessing the risk of exacerbation of apical periodontitis in root-filled teeth, undergraduate students can benefit from being trained to reflect. This study aimed to explore how students specifically reflected on clinical experiences in relation to uncertainty when assessing a case involving a root-filled tooth with apical periodontitis.</p><p><strong>Methodology: </strong>Short reflections were written by 52 dental students at Malmö University in 2021. All students were asked to reflect on the risk for exacerbation of apical periodontitis in a case with a root-filled tooth with a diffuse widening of the periodontal ligament space. The reflections were written according to prompts developed to stimulate reflection. For each student, the reflections were analysed by a qualitative method, Systematic Text Condensation (STC).</p><p><strong>Results: </strong>The analysis of the qualitative data resulted in the formulation of two themes about experience and lack of experience: Theme 1 'The meaning of clinical experience' and Theme 2 'Differences and similarities'. The themes were subdivided into nine subgroups and described the relationship between experience and certainty.</p><p><strong>Conclusions: </strong>The students believed that certainty comes with experience even when there is a lack of scientific evidence.</p>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":" ","pages":""},"PeriodicalIF":7.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To compare the accuracy and design time of artificial intelligence (AI)-generated and manually designed (MD) surgical pathways for osteotomies and root-end resections in dynamic navigation (DN)-aided endodontic microsurgery (EMS).
Methodology
Fifty-one surgical pathways were analysed, each planned using both AI and MD methodologies. Accuracy was assessed using the DCARE Navigation System (v3.2, MedNav Ltd) and AutoCAD (2023, Autodesk Inc.), evaluating five parameters: start deviation, end deviation, angular deviation, root-end resection length deviation, and root-end resection angulation deviation. Design time was measured from the point of CBCT dataset import to the finalisation of the surgical pathway design. Mann–Whitney U test was used to compare the accuracy and design time of the AI and MD groups, whereas the rank-based ANCOVA was used to assess deviations according to tooth type, jaw type, and root number. Statistical significance was set at p < 0.05.
Results
Compared with the MD group, the AI group exhibited significantly smaller root-end resection length deviations (AI: 0.01 [0.01, 0.02] mm; MD: 0.02 [0.01, 0.03] mm; p = 0.029) but significantly larger root-end resection angulation deviations (AI: 3.48 [1.01, 7.48]; MD: 0.35 [0.16, 0.73]; p < 0.001). There were no significant differences in the start deviation, end deviation, angular deviation, root-end resection length deviation, or root-end resection angulation deviation across tooth type, jaw type, or root number. The design time was significantly shorter in the AI group than in the MD group (55 [21, 74] s vs. 379 [215, 553] s; p < 0.001).
Conclusions
A clinically operational AI-based surgical path design approach is capable of minimising manual interventions and delivering time-efficient, accurate results for clinical use. The integration of AI with DN-aided EMS may contribute to the development of increasingly autonomous surgical procedures.
{"title":"Comparative Analysis of AI-Generated and Manually Designed Approaches in Accuracy and Design Time for Surgical Path Planning of Dynamic Navigation-Aided Endodontic Microsurgery: A Retrospective Study","authors":"Chen Chen, Xining Zhang, Li Qin, Liuyan Meng","doi":"10.1111/iej.70045","DOIUrl":"10.1111/iej.70045","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To compare the accuracy and design time of artificial intelligence (AI)-generated and manually designed (MD) surgical pathways for osteotomies and root-end resections in dynamic navigation (DN)-aided endodontic microsurgery (EMS).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>Fifty-one surgical pathways were analysed, each planned using both AI and MD methodologies. Accuracy was assessed using the DCARE Navigation System (v3.2, MedNav Ltd) and AutoCAD (2023, Autodesk Inc.), evaluating five parameters: start deviation, end deviation, angular deviation, root-end resection length deviation, and root-end resection angulation deviation. Design time was measured from the point of CBCT dataset import to the finalisation of the surgical pathway design. Mann–Whitney <i>U</i> test was used to compare the accuracy and design time of the AI and MD groups, whereas the rank-based ANCOVA was used to assess deviations according to tooth type, jaw type, and root number. Statistical significance was set at <i>p</i> < 0.05.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Compared with the MD group, the AI group exhibited significantly smaller root-end resection length deviations (AI: 0.01 [0.01, 0.02] mm; MD: 0.02 [0.01, 0.03] mm; <i>p</i> = 0.029) but significantly larger root-end resection angulation deviations (AI: 3.48 [1.01, 7.48]; MD: 0.35 [0.16, 0.73]; <i>p</i> < 0.001). There were no significant differences in the start deviation, end deviation, angular deviation, root-end resection length deviation, or root-end resection angulation deviation across tooth type, jaw type, or root number. The design time was significantly shorter in the AI group than in the MD group (55 [21, 74] s vs. 379 [215, 553] s; <i>p</i> < 0.001).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>A clinically operational AI-based surgical path design approach is capable of minimising manual interventions and delivering time-efficient, accurate results for clinical use. The integration of AI with DN-aided EMS may contribute to the development of increasingly autonomous surgical procedures.</p>\u0000 </section>\u0000 </div>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"59 2","pages":"288-298"},"PeriodicalIF":7.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. H. C. Lee, A. W. K. Yeung, A. Sigurdsson, M. C. M. Wong, C. F. Zhang
<div> <section> <h3> Introduction</h3> <p>There is a lack of epidemiology studies on cemental tears. This study aimed to (i) investigate the prevalence of cemental tears among the patients referred to a Postgraduate Endodontic Clinic, (ii) classify the cases diagnosed with cemental tears, and (iii) assess factors associated with cemental tears.</p> </section> <section> <h3> Materials and Methods</h3> <p>This retrospective, cross-sectional study included 445 cases/teeth recruited between 1 September 2019 and 31 March 2024 at a University Postgraduate Endodontic Clinic. Information was collated from the clinical records of case history, clinical findings and radiographic interpretations from periapical radiograph (PR) and cone beam computed tomography (CBCT) images. For each case/tooth, cemental tear/s were categorised as either absent or present, and those with cemental tear/s were further classified. Thirty potential factors were studied, including patient-, jaw-, tooth-, previous endodontic treatment and periodontal-related factors. Factors were individually tested using univariate analysis with Pearson chi-squared (exact) tests. Any significant factors identified were further subjected to multiple logistic regression analyses based on a forward stepwise regression model (<i>p</i> < 0.05).</p> </section> <section> <h3> Results</h3> <p>Cemental tear/s were found in 25 out of 445 cases/teeth, with a prevalence of 5.6%. Sensitivity and specificity of PR in detecting cemental tears were 0.6 and 1.0, respectively. Most cases/teeth detected were classified as Class 2 and 4 (<i>n</i> = 19, 76%) or Stage C and D (<i>n</i> = 19, 76%). Mean age of the subjects with cemental tears was 58.7 years, with comparable prevalence between males and females. Most cases (72%) with cemental tear/s had root canal treatment initiated or completed. Incisors, increased tooth mobility, severe crestal bone loss and adequate root filling quality were significantly associated with the higher prevalence of cemental tears (<i>p</i> < 0.05).</p> </section> <section> <h3> Conclusion</h3> <p>Cemental tears should always be considered as a differential diagnosis in endodontic practice. Clinicians should be particularly vigilant for increased risks of cemental tears in incisors, mobile teeth, teeth exhibiting increased crestal bone loss and those with adequately filled root canals, as they may be misdiagnosed as periodontal-endodontic lesions. The use of CBCT is crucial for accurate diagnosis, guiding effective treatment planning and improving clinical outcomes in endodontic ma
{"title":"Prevalence, Classification and Factors Associated With Cemental Tears—A Retrospective Observational Cross-Sectional Study in a Postgraduate Endodontic Clinic","authors":"A. H. C. Lee, A. W. K. Yeung, A. Sigurdsson, M. C. M. Wong, C. F. Zhang","doi":"10.1111/iej.70042","DOIUrl":"10.1111/iej.70042","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>There is a lack of epidemiology studies on cemental tears. This study aimed to (i) investigate the prevalence of cemental tears among the patients referred to a Postgraduate Endodontic Clinic, (ii) classify the cases diagnosed with cemental tears, and (iii) assess factors associated with cemental tears.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>This retrospective, cross-sectional study included 445 cases/teeth recruited between 1 September 2019 and 31 March 2024 at a University Postgraduate Endodontic Clinic. Information was collated from the clinical records of case history, clinical findings and radiographic interpretations from periapical radiograph (PR) and cone beam computed tomography (CBCT) images. For each case/tooth, cemental tear/s were categorised as either absent or present, and those with cemental tear/s were further classified. Thirty potential factors were studied, including patient-, jaw-, tooth-, previous endodontic treatment and periodontal-related factors. Factors were individually tested using univariate analysis with Pearson chi-squared (exact) tests. Any significant factors identified were further subjected to multiple logistic regression analyses based on a forward stepwise regression model (<i>p</i> < 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Cemental tear/s were found in 25 out of 445 cases/teeth, with a prevalence of 5.6%. Sensitivity and specificity of PR in detecting cemental tears were 0.6 and 1.0, respectively. Most cases/teeth detected were classified as Class 2 and 4 (<i>n</i> = 19, 76%) or Stage C and D (<i>n</i> = 19, 76%). Mean age of the subjects with cemental tears was 58.7 years, with comparable prevalence between males and females. Most cases (72%) with cemental tear/s had root canal treatment initiated or completed. Incisors, increased tooth mobility, severe crestal bone loss and adequate root filling quality were significantly associated with the higher prevalence of cemental tears (<i>p</i> < 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Cemental tears should always be considered as a differential diagnosis in endodontic practice. Clinicians should be particularly vigilant for increased risks of cemental tears in incisors, mobile teeth, teeth exhibiting increased crestal bone loss and those with adequately filled root canals, as they may be misdiagnosed as periodontal-endodontic lesions. The use of CBCT is crucial for accurate diagnosis, guiding effective treatment planning and improving clinical outcomes in endodontic ma","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"59 1","pages":"57-79"},"PeriodicalIF":7.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/iej.70042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This randomised clinical trial compared the effect of wound lavage using chlorhexidine (CHX) versus sodium hypochlorite (NaOCl) on the outcome of partial pulpotomy (PP) in mature permanent molars with carious pulpal exposure and symptomatic irreversible pulpitis (SIP).
Methodology
In this double-arm parallel-group randomised clinical trial, 104 mandibular molars undergoing PP were randomised equally to either NaOCl lavage group (NL) (n = 52) or CHX lavage group (CL) (n = 52). The pulpal wound after pulp amputation during PP was irrigated with 3% NaOCl in the NL and 2% CHX in the CL group. After haemostasis, ProRoot Mineral Trioxide Aggregate (ProRoot MTA) was used as a pulpotomy agent. Patients were evaluated for pain experience at every 24 h for 7 days, clinical success at 3, 6 and 12 months, and radiographic success at 6 and 12 months. Data were analysed using Mann–Whitney U test to compare age, duration of haemostasis, pain score and analgesic consumption between groups and Chi-square test/Fisher's exact test to compare gender, the number of tooth surfaces involved by caries, hard tissue barrier, clinical and radiographic success, pain incidence and pulp sensibility responses.
Results
Ninety-nine patients were assessed for clinical and radiographic success at 12 months follow-up. The NL group showed a higher success (91.8%) than the CL group (84%), albeit with no significant difference (p > 0.05). No significant difference was observed between the groups in postoperative pain experience (p > 0.05). Significantly less time was required to achieve haemostasis in NL (p < 0.001), and evidence of hard tissue barrier formation was significantly higher in the CL group (p = 0.024).
Conclusion
Comparable success was observed following the use of the two lavage agents during PP in managing cariously exposed adult teeth with SIP. CHX can serve as a suitable alternative to NaOCl as a pulpal wound lavage agent.
{"title":"Outcome of Partial Pulpotomy in Mature Permanent Molars After Lavage With Chlorhexidine or Sodium Hypochlorite: A Randomised Clinical Trial","authors":"Soumyashree Mishra, Pankaj Sangwan, Jigyasa Duhan, Mayank Arora, Shweta Mittal, Vinay Kumar, Ankita Ramani","doi":"10.1111/iej.70043","DOIUrl":"10.1111/iej.70043","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>This randomised clinical trial compared the effect of wound lavage using chlorhexidine (CHX) versus sodium hypochlorite (NaOCl) on the outcome of partial pulpotomy (PP) in mature permanent molars with carious pulpal exposure and symptomatic irreversible pulpitis (SIP).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methodology</h3>\u0000 \u0000 <p>In this double-arm parallel-group randomised clinical trial, 104 mandibular molars undergoing PP were randomised equally to either NaOCl lavage group (NL) (<i>n</i> = 52) or CHX lavage group (CL) (<i>n</i> = 52). The pulpal wound after pulp amputation during PP was irrigated with 3% NaOCl in the NL and 2% CHX in the CL group. After haemostasis, ProRoot Mineral Trioxide Aggregate (ProRoot MTA) was used as a pulpotomy agent. Patients were evaluated for pain experience at every 24 h for 7 days, clinical success at 3, 6 and 12 months, and radiographic success at 6 and 12 months. Data were analysed using Mann–Whitney <i>U</i> test to compare age, duration of haemostasis, pain score and analgesic consumption between groups and Chi-square test/Fisher's exact test to compare gender, the number of tooth surfaces involved by caries, hard tissue barrier, clinical and radiographic success, pain incidence and pulp sensibility responses.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Ninety-nine patients were assessed for clinical and radiographic success at 12 months follow-up. The NL group showed a higher success (91.8%) than the CL group (84%), albeit with no significant difference (<i>p</i> > 0.05). No significant difference was observed between the groups in postoperative pain experience (<i>p</i> > 0.05). Significantly less time was required to achieve haemostasis in NL (<i>p</i> < 0.001), and evidence of hard tissue barrier formation was significantly higher in the CL group (<i>p</i> = 0.024).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Comparable success was observed following the use of the two lavage agents during PP in managing cariously exposed adult teeth with SIP. CHX can serve as a suitable alternative to NaOCl as a pulpal wound lavage agent.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>clinicaltrials.gov identifier: NCT06240130</p>\u0000 </section>\u0000 </div>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"59 1","pages":"80-90"},"PeriodicalIF":7.1,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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