Tao Zhou, Yiyi Huang, Xiaoyu Ling, Lei Zhang, Qing Gu, Dafeng Song
Truncation and amino acid residue substitution are common methods to optimise the design of antimicrobial peptides (AMPs). In the present work, seven truncated and residue-substituted derivatives of Plantaricin ZJ5 (PZJ5) were designed and synthesised. PZJ5-5 was a truncation that simultaneously contained three substituted amino-acid residues, with enhanced antimicrobial activity and low haemolytic activity. The effects of PZJ5-5 on Escherichia coli microstructure were investigated using scanning and transmission electron microscopy, which indicated that its antibacterial mechanism was similar to PZJ5. C-terminal amidation of PZJ5-5 (PZJ5-7) was deleterious, and resulted in a dramatic reduction in potency against E. coli and Listeria monocytogenes, with no potency against the other three indicator bacteria. Truncation and residue substitution of bacteriocin PZJ5 changed its antimicrobial activities and specificities, which provided a rationale for bacteriocin design.
{"title":"Antimicrobial activities and mechanisms of truncated and amino-acid-substituted peptides derived from bacteriocin PZJ5","authors":"Tao Zhou, Yiyi Huang, Xiaoyu Ling, Lei Zhang, Qing Gu, Dafeng Song","doi":"10.47836/ifrj.30.6.20","DOIUrl":"https://doi.org/10.47836/ifrj.30.6.20","url":null,"abstract":"Truncation and amino acid residue substitution are common methods to optimise the design of antimicrobial peptides (AMPs). In the present work, seven truncated and residue-substituted derivatives of Plantaricin ZJ5 (PZJ5) were designed and synthesised. PZJ5-5 was a truncation that simultaneously contained three substituted amino-acid residues, with enhanced antimicrobial activity and low haemolytic activity. The effects of PZJ5-5 on Escherichia coli microstructure were investigated using scanning and transmission electron microscopy, which indicated that its antibacterial mechanism was similar to PZJ5. C-terminal amidation of PZJ5-5 (PZJ5-7) was deleterious, and resulted in a dramatic reduction in potency against E. coli and Listeria monocytogenes, with no potency against the other three indicator bacteria. Truncation and residue substitution of bacteriocin PZJ5 changed its antimicrobial activities and specificities, which provided a rationale for bacteriocin design.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"85 24","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138956678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manufacturers’ desire to sell “healthy” food in response to the consumers’ desire to lead a healthy lifestyle has increased the use of probiotics during the past few decades. Probiotics are used in dairy products, as well as non-dairy items as a starter culture, encompassing a wide range of goods. Numerous phenotyping, physical characterisation, and genotyping techniques have been developed to identify probiotic lactobacilli to ensure quality management. These techniques are frequently precise enough to categorise probiotic strains by genus and species. Traditional microbiological methods were initially employed for genus and species identification. However, due to their numerous shortcomings as the probiotic ability is often strain-dependent, and that there is no way to differentiate between strains using simple microbiological techniques, new methods that are mostly based on the examination of nucleic acids have been developed. Therefore, the objective of the present review was to provide critical assessment on existing methods for identifying members of the genus Lactobacillus, together with newly discovered approaches. The present review aimed to give the most recent information on the scientific techniques used to measure and describe the possible probiotic properties of microorganisms. It will also emphasise molecular and non-molecular tools. Most of these tools are based on 16S ribosomal DNA sequencing, and employ PCR techniques.
{"title":"Comparative study on biochemical and molecular identification approaches of Lactobacillus species","authors":"Disha P. Senjaliya, John J. Georrge","doi":"10.47836/ifrj.30.5.03","DOIUrl":"https://doi.org/10.47836/ifrj.30.5.03","url":null,"abstract":"Manufacturers’ desire to sell “healthy” food in response to the consumers’ desire to lead a healthy lifestyle has increased the use of probiotics during the past few decades. Probiotics are used in dairy products, as well as non-dairy items as a starter culture, encompassing a wide range of goods. Numerous phenotyping, physical characterisation, and genotyping techniques have been developed to identify probiotic lactobacilli to ensure quality management. These techniques are frequently precise enough to categorise probiotic strains by genus and species. Traditional microbiological methods were initially employed for genus and species identification. However, due to their numerous shortcomings as the probiotic ability is often strain-dependent, and that there is no way to differentiate between strains using simple microbiological techniques, new methods that are mostly based on the examination of nucleic acids have been developed. Therefore, the objective of the present review was to provide critical assessment on existing methods for identifying members of the genus Lactobacillus, together with newly discovered approaches. The present review aimed to give the most recent information on the scientific techniques used to measure and describe the possible probiotic properties of microorganisms. It will also emphasise molecular and non-molecular tools. Most of these tools are based on 16S ribosomal DNA sequencing, and employ PCR techniques.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"17 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135265617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Addition of whole quinoa flour (WQF) into wheat flour is a promising approach to make quinoa food efficient, but the amount of WQF addition has a great influence on the quality of the quinoa food. The water-soluble index, swelling power, pasting property, dough rheological properties, and steam bread textural property of wheat flour containing 10 - 50% (w/w) WQF were investigated in the present work. Results showed that water soluble index and swelling power of mixed flour decreased gradually as the WQF content increased. Mixed flour containing higher WQF content (30 - 50%) had a lower peak viscosity, breakdown, and setback value than mixed flour containing lower content WQF (10 - 20%). Doughs containing 30 - 50% WQF had a weaker dough stability, less dough development time, but an increased water absorption and softening degree than the doughs containing 10 - 20% WQF. Dough’s extension resistance increased and then decreased with the addition of WQF. Dough energy and extensibility of the mixed flour decreased with increasing level of WQF (10 - 50%). In addition, hardness and chewiness of steam bread made by mixed flour increased with increasing amount of WQF (10 - 50%). In view of the practical point, the maximum addition level of WQF was 20% when making a quinoa-wheat steam bread without any food additives.
{"title":"Effects of whole quinoa flour addition on the pasting property, dough rheology, and steam bread textural property of wheat flour","authors":"Ya Yun Feng, YiLin Zhu, Zhuo Wang, XiaoLong Li","doi":"10.47836/ifrj.30.5.10","DOIUrl":"https://doi.org/10.47836/ifrj.30.5.10","url":null,"abstract":"Addition of whole quinoa flour (WQF) into wheat flour is a promising approach to make quinoa food efficient, but the amount of WQF addition has a great influence on the quality of the quinoa food. The water-soluble index, swelling power, pasting property, dough rheological properties, and steam bread textural property of wheat flour containing 10 - 50% (w/w) WQF were investigated in the present work. Results showed that water soluble index and swelling power of mixed flour decreased gradually as the WQF content increased. Mixed flour containing higher WQF content (30 - 50%) had a lower peak viscosity, breakdown, and setback value than mixed flour containing lower content WQF (10 - 20%). Doughs containing 30 - 50% WQF had a weaker dough stability, less dough development time, but an increased water absorption and softening degree than the doughs containing 10 - 20% WQF. Dough’s extension resistance increased and then decreased with the addition of WQF. Dough energy and extensibility of the mixed flour decreased with increasing level of WQF (10 - 50%). In addition, hardness and chewiness of steam bread made by mixed flour increased with increasing amount of WQF (10 - 50%). In view of the practical point, the maximum addition level of WQF was 20% when making a quinoa-wheat steam bread without any food additives.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135265623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cuihua Chang, Ali Ahmad Leghari, Xin Li, Yujie Su, Luping Gu, Yanjun Yang, Junhua Li
Egg yolk immunoglobulin (IgY) is a biologically active ingredient with high immunogenicity; however, its instability in the acidic environment of the upper gastrointestinal tract limits its application in oral formulations. In the present work, an encapsulation system based on maltodextrin (MD) and konjac gum (KGM) was developed as a protective carrier for IgY for targeted release to retain stability. A simulated gastric model was used to compare the release characteristics of the different formulations, and to explore the optimal release mode. To better understand the controlled release mechanism of MD and KGM composite tablets, the release curve, macrostructure, microstructure, and water mobility were analysed. Results indicated that the sustained release of IgY from MD and KGM composite tablets was mainly driven by Fick diffusion and dissolution. As the concentration of KGM increased, the release rate of IgY from the tablets decreased, and the release mechanism gradually changed from diffusion to erosion. The dense cross-linkage between MD and KGM helped prevent tablet disintegration, and slowed down the release of IgY. In addition, the dissolved KGM formed a film on the tablet surface to control the erosion rate. It can be concluded that the optimal formulation of an IgY-loaded tablet was a mass fraction of 50% MD, 30% KGM, and 20% IgY. The present work provided a practical method to protect the biologically active ingredients from acidic destruction in the stomach during oral treatment.
{"title":"Konjac gum and maltodextrin compound tablets as carriers of IgY for sustained release in stomach","authors":"Cuihua Chang, Ali Ahmad Leghari, Xin Li, Yujie Su, Luping Gu, Yanjun Yang, Junhua Li","doi":"10.47836/ifrj.30.5.17","DOIUrl":"https://doi.org/10.47836/ifrj.30.5.17","url":null,"abstract":"Egg yolk immunoglobulin (IgY) is a biologically active ingredient with high immunogenicity; however, its instability in the acidic environment of the upper gastrointestinal tract limits its application in oral formulations. In the present work, an encapsulation system based on maltodextrin (MD) and konjac gum (KGM) was developed as a protective carrier for IgY for targeted release to retain stability. A simulated gastric model was used to compare the release characteristics of the different formulations, and to explore the optimal release mode. To better understand the controlled release mechanism of MD and KGM composite tablets, the release curve, macrostructure, microstructure, and water mobility were analysed. Results indicated that the sustained release of IgY from MD and KGM composite tablets was mainly driven by Fick diffusion and dissolution. As the concentration of KGM increased, the release rate of IgY from the tablets decreased, and the release mechanism gradually changed from diffusion to erosion. The dense cross-linkage between MD and KGM helped prevent tablet disintegration, and slowed down the release of IgY. In addition, the dissolved KGM formed a film on the tablet surface to control the erosion rate. It can be concluded that the optimal formulation of an IgY-loaded tablet was a mass fraction of 50% MD, 30% KGM, and 20% IgY. The present work provided a practical method to protect the biologically active ingredients from acidic destruction in the stomach during oral treatment.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"38 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135265629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present work was performed to evaluate the potency of papaya leaf extract (PLE) to improve the characteristics of cornhusk as a packaging material for glutinous rice snack (GRS). Total phenolic, tannin, and saponin contents of PLE were 7.41 ± 0.65 mgTAE/mL, 1.80 ± 0.70 mgTAE/mL, and 11.78 ± 0.36 mgDE/mL, respectively. The presence of bioactive compounds on the surface of GRS was confirmed by Fourier transform infrared spectroscopy analysis. Characteristic bands for saponin were caused by the stretching vibration of C=O (at 1744 cm-1) as well as C‒O and C‒C vibrations (at 1051 cm-1). Tannin was identified as C‒O asymmetric stretch vibration at 1051 cm-1 and C‒H out of plane vibration at 926 and 866-867 cm-1. The antioxidant activity of PLE was found to be 49.53 ± 2.67%. The reductions of total plate counts (TPC), yeast and mould counts (YMC), and Aspergillus flavus-A. parasiticus counts on PLE-incorporated cornhusks after 24 h were in the range of 0.2 - 1.2 log CFU/cm2, and retained the loads below 2 log CFU/cm2 during 14-d storage. PLE decreased the water vapour transmission rate of the cornhusk due to the particles of the extract adhering to the cornhusk surface, as supported by the result of the scanning electron microscopy of PLE-incorporated cornhusk. The incorporation of PLE also increased elongation without reducing the tensile strength of the cornhusk significantly. There were reductions of TPC, YMC, and Aspergillus flavus-A. parasiticus counts of GRS ranging from 0.4 - 2.3 log CFU/g using PLE-incorporated cornhusks during storage. GRS rancidity was minimised by PLE-incorporated cornhusks. Owing to its bioactive compound, PLE could be incorporated into the cornhusk to improve packaging characteristics and controls microbial contamination of GRS while retarding the rancidity.
{"title":"Incorporation of papaya (Carica papaya L.) leaf extract into cornhusk for glutinous rice snack packaging application","authors":"Aji Sukoco, Nugraha Edhi Suyatma, Harsi Dewantari Kusumaningrum","doi":"10.47836/ifrj.30.5.11","DOIUrl":"https://doi.org/10.47836/ifrj.30.5.11","url":null,"abstract":"The present work was performed to evaluate the potency of papaya leaf extract (PLE) to improve the characteristics of cornhusk as a packaging material for glutinous rice snack (GRS). Total phenolic, tannin, and saponin contents of PLE were 7.41 ± 0.65 mgTAE/mL, 1.80 ± 0.70 mgTAE/mL, and 11.78 ± 0.36 mgDE/mL, respectively. The presence of bioactive compounds on the surface of GRS was confirmed by Fourier transform infrared spectroscopy analysis. Characteristic bands for saponin were caused by the stretching vibration of C=O (at 1744 cm-1) as well as C‒O and C‒C vibrations (at 1051 cm-1). Tannin was identified as C‒O asymmetric stretch vibration at 1051 cm-1 and C‒H out of plane vibration at 926 and 866-867 cm-1. The antioxidant activity of PLE was found to be 49.53 ± 2.67%. The reductions of total plate counts (TPC), yeast and mould counts (YMC), and Aspergillus flavus-A. parasiticus counts on PLE-incorporated cornhusks after 24 h were in the range of 0.2 - 1.2 log CFU/cm2, and retained the loads below 2 log CFU/cm2 during 14-d storage. PLE decreased the water vapour transmission rate of the cornhusk due to the particles of the extract adhering to the cornhusk surface, as supported by the result of the scanning electron microscopy of PLE-incorporated cornhusk. The incorporation of PLE also increased elongation without reducing the tensile strength of the cornhusk significantly. There were reductions of TPC, YMC, and Aspergillus flavus-A. parasiticus counts of GRS ranging from 0.4 - 2.3 log CFU/g using PLE-incorporated cornhusks during storage. GRS rancidity was minimised by PLE-incorporated cornhusks. Owing to its bioactive compound, PLE could be incorporated into the cornhusk to improve packaging characteristics and controls microbial contamination of GRS while retarding the rancidity.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"29 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135266645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zingiber cassumunar Roxb. (ZC) and purple sweet potato (PSP) are the potential sources of antioxidants, and play an important role in reducing blood fat levels, and encourage the development of antihyperlipidemic products, usually in the form of biscuits. Biscuits are favoured by consumers because of their delicious and varied tastes, relatively cheap prices, and complete nutritional content. In the present work, biscuits were made with three formula variations of ZC:PSP ratios namely F1 (0.75:5.25 g), F2 (0.45:5.55 g), and F3 (0.2:5.72 g), and then tested for their physical quality based on Indonesian National Standard (SNI), and hedonic testing to get the best formula, including aroma, colour, texture, and taste. Furthermore, in vivo antihyperlipidemic testing was carried out on the best formula. The test was conducted by dividing Sprague-Dawley rats into four groups, namely normal, negative, positive (Nutrive Benecol animal, 3.6 mL/day for 28 days), and the best formula (1.944 g/day for 28 days). All groups were induced with high fat diet (HFD) for 28 days, except normal group. Results showed a decrease in cholesterol, triglyceride, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SGPT) levels in the normal group with values of 99.09 mg/dL, 90.36 mg/dL, 22.66 U/I, and 39.41 U/I, respectively, as compared to the negative group with values of 195.01 mg/dL, 142.44 mg/dL, 29.05 U/I, and 77.19 U/I, respectively. There was an increase in cholesterol, triglyceride, SGOT, and SGPT levels in the positive group with values of 108.39 mg/dL, 96.12 mg/dL, 24.11 U/I, and 48.55 U/I, respectively, as compared to the normal group test. A combination of biscuits made from ZC and PSP flour could have the potential to reduce lipid levels in the blood, and encourage the development of antihyperlipidemic products.
{"title":"Development of biscuits from bangle (Zingiber cassumunar Roxb.) rhizome flour and purple sweet potato (Ipomoea batatas (L.) Lam.) flour, and their potential as antihyperlipidemic functional foods","authors":"Nurkhasanah Mahfudh, Liza Wardani, Ika Dyah Kumalasari, Nanik Sulistyani","doi":"10.47836/ifrj.30.5.04","DOIUrl":"https://doi.org/10.47836/ifrj.30.5.04","url":null,"abstract":"Zingiber cassumunar Roxb. (ZC) and purple sweet potato (PSP) are the potential sources of antioxidants, and play an important role in reducing blood fat levels, and encourage the development of antihyperlipidemic products, usually in the form of biscuits. Biscuits are favoured by consumers because of their delicious and varied tastes, relatively cheap prices, and complete nutritional content. In the present work, biscuits were made with three formula variations of ZC:PSP ratios namely F1 (0.75:5.25 g), F2 (0.45:5.55 g), and F3 (0.2:5.72 g), and then tested for their physical quality based on Indonesian National Standard (SNI), and hedonic testing to get the best formula, including aroma, colour, texture, and taste. Furthermore, in vivo antihyperlipidemic testing was carried out on the best formula. The test was conducted by dividing Sprague-Dawley rats into four groups, namely normal, negative, positive (Nutrive Benecol animal, 3.6 mL/day for 28 days), and the best formula (1.944 g/day for 28 days). All groups were induced with high fat diet (HFD) for 28 days, except normal group. Results showed a decrease in cholesterol, triglyceride, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SGPT) levels in the normal group with values of 99.09 mg/dL, 90.36 mg/dL, 22.66 U/I, and 39.41 U/I, respectively, as compared to the negative group with values of 195.01 mg/dL, 142.44 mg/dL, 29.05 U/I, and 77.19 U/I, respectively. There was an increase in cholesterol, triglyceride, SGOT, and SGPT levels in the positive group with values of 108.39 mg/dL, 96.12 mg/dL, 24.11 U/I, and 48.55 U/I, respectively, as compared to the normal group test. A combination of biscuits made from ZC and PSP flour could have the potential to reduce lipid levels in the blood, and encourage the development of antihyperlipidemic products.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"12 4-5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135265619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Atna Permana, Nurzafirah Mazlan, E. Yusuf, S.M. Anua, T.Y. Thang, A. Baktir
Antibiotic resistance occurs worldwide, and has become a threat to humankind. Previous data have shown that antimicrobial resistance is a global issue demanding immediate resolution because it threatens the environment and society. The present work thus investigated the synergistic effects of commercial Garcinia mangostana L. (GML) extract and amoxicillin on the growth of methicillin-resistant Staphylococcus aureus (MRSA) bacterial cells. A commercial GML extract was screened for phytochemical properties, and the presence of α-mangostin was detected using high-performance liquid chromatography (HPLC). The antibacterial activity of the commercial GML extract with amoxicillin was analysed by minimum inhibitory concentration (MIC) and checkerboard assays. The morphology ultrastructure of bacteria was observed using transmission electron microscopy (TEM), after treatment with commercial GML extract, either single or in combination with amoxicillin. The MICs of amoxicillin and commercial GML extract against MRSA bacteria were 250.00 and 137.50 μg/mL, respectively. The checkerboard assay showed synergistic activity in the combination of commercial GML extract (34.38 µg/mL) and amoxicillin (62.50 µg/mL) at fractional inhibitory concentration (FIC) index of < 0.5. Damage to the structure of bacteria occurred due to the commercial GML extract plus amoxicillin. It was observed that the loss of bacterial cell membranes led to an irregular bacterial structure. These findings provided evidence that the combination of commercial GML extract and amoxicillin could reverse bacterial resistance in order to determine the susceptibility of traditional drugs.
{"title":"Synergistic effect of commercial mangosteen extract (Garcinia mangostana L.) and amoxicillin against methicillin-resistant Staphylococcus aureus (MRSA)","authors":"Atna Permana, Nurzafirah Mazlan, E. Yusuf, S.M. Anua, T.Y. Thang, A. Baktir","doi":"10.47836/ifrj.30.5.19","DOIUrl":"https://doi.org/10.47836/ifrj.30.5.19","url":null,"abstract":"Antibiotic resistance occurs worldwide, and has become a threat to humankind. Previous data have shown that antimicrobial resistance is a global issue demanding immediate resolution because it threatens the environment and society. The present work thus investigated the synergistic effects of commercial Garcinia mangostana L. (GML) extract and amoxicillin on the growth of methicillin-resistant Staphylococcus aureus (MRSA) bacterial cells. A commercial GML extract was screened for phytochemical properties, and the presence of α-mangostin was detected using high-performance liquid chromatography (HPLC). The antibacterial activity of the commercial GML extract with amoxicillin was analysed by minimum inhibitory concentration (MIC) and checkerboard assays. The morphology ultrastructure of bacteria was observed using transmission electron microscopy (TEM), after treatment with commercial GML extract, either single or in combination with amoxicillin. The MICs of amoxicillin and commercial GML extract against MRSA bacteria were 250.00 and 137.50 μg/mL, respectively. The checkerboard assay showed synergistic activity in the combination of commercial GML extract (34.38 µg/mL) and amoxicillin (62.50 µg/mL) at fractional inhibitory concentration (FIC) index of < 0.5. Damage to the structure of bacteria occurred due to the commercial GML extract plus amoxicillin. It was observed that the loss of bacterial cell membranes led to an irregular bacterial structure. These findings provided evidence that the combination of commercial GML extract and amoxicillin could reverse bacterial resistance in order to determine the susceptibility of traditional drugs.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"34 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135265624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zahrah-Izati Azhar Shapawi, Siti Hajar Ariffin, Rosnah Shamsudin, Intan Syafinaz Mohammed Amin Tawakkal, Mohd Salahuddin Mohd Basri
Dabai (Canarium odontophyllum Miq.) is a highly nutritious fruit that has a huge potential to be marketed both locally and globally. However, the lack of promotion leads to an oversupply during peak season, thus reducing its market price. Proper handling and packaging are therefore necessary to maintain the quality and extend the shelf life of dabai. In the present work, nylon film with an oxygen transmission rate (OTR) of 55 cc/m2/day and water vapour transmission rate (WVTR) of 334 g/m2/day; polyethylene terephthalate (PET) film with an OTR of 90 cc/m2/day and WVTR of 35 g/m2/day; and low-density polyethylene (LDPE) film with an OTR of 8000 cc/m2/day and WVTR of 200 g/m2/day were used to pack dabai and stored at 5°C for 14 d. All films had a dimension of 200 × 300 mm, and a thickness of 0.01 μm. It was found that dabai maintained its hue angle (h°) values within the dark purple region (299.73° to 338.64°) and its lightness (L*) values throughout storage. However, the colour intensity (chroma) significantly changed (p < 0.05) between different films throughout storage (p < 0.05). The control sample had the most significant decrease in firmness and weight (p < 0.05) between day 0 and 14, followed by the samples stored in PET, LDPE, and nylon. Whereas the samples in LDPE demonstrated the lowest respiration rate as compared to nylon and PET. The uncompetitive Michaelis-Menten equation model was used to model the respiration rate of dabai. Results showed that all films obtained good fit (R2 of near to 1). Additionally, the mean relative percentage (E%) was less than 10%, thus indicating that the data were suitable for real-time application.
{"title":"Modelling the respiration rate of dabai fruit (Canarium odontophyllum Miq.) stored in different packaging films","authors":"Zahrah-Izati Azhar Shapawi, Siti Hajar Ariffin, Rosnah Shamsudin, Intan Syafinaz Mohammed Amin Tawakkal, Mohd Salahuddin Mohd Basri","doi":"10.47836/ifrj.30.5.20","DOIUrl":"https://doi.org/10.47836/ifrj.30.5.20","url":null,"abstract":"Dabai (Canarium odontophyllum Miq.) is a highly nutritious fruit that has a huge potential to be marketed both locally and globally. However, the lack of promotion leads to an oversupply during peak season, thus reducing its market price. Proper handling and packaging are therefore necessary to maintain the quality and extend the shelf life of dabai. In the present work, nylon film with an oxygen transmission rate (OTR) of 55 cc/m2/day and water vapour transmission rate (WVTR) of 334 g/m2/day; polyethylene terephthalate (PET) film with an OTR of 90 cc/m2/day and WVTR of 35 g/m2/day; and low-density polyethylene (LDPE) film with an OTR of 8000 cc/m2/day and WVTR of 200 g/m2/day were used to pack dabai and stored at 5°C for 14 d. All films had a dimension of 200 × 300 mm, and a thickness of 0.01 μm. It was found that dabai maintained its hue angle (h°) values within the dark purple region (299.73° to 338.64°) and its lightness (L*) values throughout storage. However, the colour intensity (chroma) significantly changed (p < 0.05) between different films throughout storage (p < 0.05). The control sample had the most significant decrease in firmness and weight (p < 0.05) between day 0 and 14, followed by the samples stored in PET, LDPE, and nylon. Whereas the samples in LDPE demonstrated the lowest respiration rate as compared to nylon and PET. The uncompetitive Michaelis-Menten equation model was used to model the respiration rate of dabai. Results showed that all films obtained good fit (R2 of near to 1). Additionally, the mean relative percentage (E%) was less than 10%, thus indicating that the data were suitable for real-time application.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"46 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135265781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cheeses have different characteristics based on the coagulant used during their production. Making suspesi soft cheese by adding biduri (Calotropis gigantea) leaf extract is generally followed by heating. The present work focused only on the ability of biduri leaf extracts to coagulate milk protein, and not on heating of the extract. The present work aimed to analyse the coagulation power of fresh and powdered biduri leaf extracts, and to evaluate the physicochemical, chemical, and organoleptic characteristics of the produced suspesi soft cheeses. The present work used a completely randomised design with two treatments and six replications. The variables measured were physicochemical characteristics, including coagulation time, curd production, whey percentage, pH, and lactic acid titration. Physicochemical characteristic analyses of suspesi soft cheeses coagulated with fresh and powdered biduri leaf extracts showed significant differences (p < 0.05) in the coagulation time and curd production. On the other hand, it did not show significant differences (p > 0.05) in the percentage of whey, pH, and lactic acid titration. The chemical content of suspesi soft cheeses showed significant differences (p < 0.05) in the water, protein, and crude fat contents. The organoleptic characteristics of suspesi soft cheeses showed a significant difference (p < 0.05) in the colour, but did not show significant differences (p > 0.05) in the smell, taste, and texture. Suspesi soft cheese produced with fresh biduri leaf extract had better coagulation power, physicochemical characteristics, chemical content, and organoleptic characteristics.
{"title":"Coagulation power comparison between fresh and powdered biduri (Calotropis gigantea) leaf extract in making suspesi soft cheese","authors":"S. Sulmiyati, G.E.M. Malelak","doi":"10.47836/ifrj.30.5.22","DOIUrl":"https://doi.org/10.47836/ifrj.30.5.22","url":null,"abstract":"Cheeses have different characteristics based on the coagulant used during their production. Making suspesi soft cheese by adding biduri (Calotropis gigantea) leaf extract is generally followed by heating. The present work focused only on the ability of biduri leaf extracts to coagulate milk protein, and not on heating of the extract. The present work aimed to analyse the coagulation power of fresh and powdered biduri leaf extracts, and to evaluate the physicochemical, chemical, and organoleptic characteristics of the produced suspesi soft cheeses. The present work used a completely randomised design with two treatments and six replications. The variables measured were physicochemical characteristics, including coagulation time, curd production, whey percentage, pH, and lactic acid titration. Physicochemical characteristic analyses of suspesi soft cheeses coagulated with fresh and powdered biduri leaf extracts showed significant differences (p < 0.05) in the coagulation time and curd production. On the other hand, it did not show significant differences (p > 0.05) in the percentage of whey, pH, and lactic acid titration. The chemical content of suspesi soft cheeses showed significant differences (p < 0.05) in the water, protein, and crude fat contents. The organoleptic characteristics of suspesi soft cheeses showed a significant difference (p < 0.05) in the colour, but did not show significant differences (p > 0.05) in the smell, taste, and texture. Suspesi soft cheese produced with fresh biduri leaf extract had better coagulation power, physicochemical characteristics, chemical content, and organoleptic characteristics.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"60 5-6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135265784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gabriela Niemeyer Reissig, Thiago Francisco de Carvalho Oliveira, Guilherme Cassão Marques Bragança, Rosane da Silva Rodrigues, Frabrizio Da Fonseca Barbosa
There is a trend towards the consumption of plant foods, especially from the public that aims to reduce meat consumption. Plant-based food diets may have low source of vitamin B12 as plants do not produce them. A possible alternative to mitigate this is the consumption of fermented vegetables and fruits. Therefore, we aimed to provide an overview of the work being done with fermented vegetables and fruits, and show evidence that it is possible to obtain the necessary daily amount of vitamin B12 for human health and maintenance. Vitamin B12, also known as cobalamin, acts as a cofactor for the enzyme methionine synthase and methylmalonylCoA mutase in eukaryotes. The dietary reference values for adult men and women range between 2 and 4 μg/day; however, the requirement may increase depending on special recommendations. The main causes for vitamin B12 deficiency are autoimmune conditions (e.g., pernicious anaemia), malabsorption, and dietary insufficiency. One of the commonly adopted measures to deal with vitamin deficiency is supplementation. It is also possible to obtain food enriched with vitamin B12 through fermentation. Different plant materials and microorganisms can be used to produce fermented products and enhance traditional products, such as tempeh, to increase vitamin B12 concentration in the final product. The bioaccessibility and bioavailability of vitamin B12 in fermented vegetables and fruits are important factors to be considered, and demand more studies. The intake of soy fermented foods, such as tempeh, tofu, and cheonggukjang was associated with cognitive enhancement and neuroprotective effects. In addition to fermented vegetables and fruits, other non-animal sources of vitamin B12 that deserve great attention are algae and mushrooms. Since fermentation can produce considerable amounts of vitamin B12, fermented vegetables and fruits are feasible alternative sources for the intake of this vitamin.
{"title":"Fermented vegetables and fruits as vitamin B12 sources: An overview","authors":"Gabriela Niemeyer Reissig, Thiago Francisco de Carvalho Oliveira, Guilherme Cassão Marques Bragança, Rosane da Silva Rodrigues, Frabrizio Da Fonseca Barbosa","doi":"10.47836/ifrj.30.5.02","DOIUrl":"https://doi.org/10.47836/ifrj.30.5.02","url":null,"abstract":"There is a trend towards the consumption of plant foods, especially from the public that aims to reduce meat consumption. Plant-based food diets may have low source of vitamin B12 as plants do not produce them. A possible alternative to mitigate this is the consumption of fermented vegetables and fruits. Therefore, we aimed to provide an overview of the work being done with fermented vegetables and fruits, and show evidence that it is possible to obtain the necessary daily amount of vitamin B12 for human health and maintenance. Vitamin B12, also known as cobalamin, acts as a cofactor for the enzyme methionine synthase and methylmalonylCoA mutase in eukaryotes. The dietary reference values for adult men and women range between 2 and 4 μg/day; however, the requirement may increase depending on special recommendations. The main causes for vitamin B12 deficiency are autoimmune conditions (e.g., pernicious anaemia), malabsorption, and dietary insufficiency. One of the commonly adopted measures to deal with vitamin deficiency is supplementation. It is also possible to obtain food enriched with vitamin B12 through fermentation. Different plant materials and microorganisms can be used to produce fermented products and enhance traditional products, such as tempeh, to increase vitamin B12 concentration in the final product. The bioaccessibility and bioavailability of vitamin B12 in fermented vegetables and fruits are important factors to be considered, and demand more studies. The intake of soy fermented foods, such as tempeh, tofu, and cheonggukjang was associated with cognitive enhancement and neuroprotective effects. In addition to fermented vegetables and fruits, other non-animal sources of vitamin B12 that deserve great attention are algae and mushrooms. Since fermentation can produce considerable amounts of vitamin B12, fermented vegetables and fruits are feasible alternative sources for the intake of this vitamin.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"18 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135265789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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