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Multiresidue analysis and health risk assessment of sulfonamides and quinolones from edible Batrachia and other aquatic products 食用蝙蝠蛾和其他水产品中的磺胺类和喹诺酮类药物的多残留分析和健康风险评估
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.09
Huang Hu, Zhongyong Yan, Shiyan Li, Zitong Zhu, Wanzheng Li, Jingjing Tai, Hong Zhang
Tailless amphibians belonging to the order Batrachia are a significant component of human consumption. However, there is limited literature on the consumption of these amphibians by humans. Therefore, the present work aimed to examine the presence of drug residues in edible Batrachia, and compare them with other aquatic products. We assessed 22 veterinary drug residues, including 12 sulfonamides (SAs) and ten quinolones (QNs), in various aquatic products, including edible Batrachia. To provide a comparative analysis, we referenced literature from China between 2005 and 2020 regarding SAs and QNs detected in aquatic products. Additionally, we calculated the food safety index (IFS) of antibiotics, and conducted a health risk assessment. The findings revealed that the detection rate, average residual amount, and over-standard rate of antibiotics in edible Batrachia were higher than most other aquatic products. Furthermore, the systematic cluster analysis demonstrated that edible Batrachia could serve as a potential sentinel animal, distinguishing them from several other aquatic products. The total IFS of all residues obtained from different aquatic products in the present work was less than 1, thus indicating their safety in terms of public health. Nevertheless, it is essential to conduct detailed investigations into the maximum residues of certain antibiotics in specific aquatic products that exceed the safety limits to understand their impact on human health.
属于蝙蝠纲的无尾两栖动物是人类消费的重要组成部分。然而,有关人类食用这些两栖动物的文献却很有限。因此,本研究旨在检测可食用蝙蝠蛾中是否存在药物残留,并将其与其他水产品进行比较。我们评估了包括食用蝙蝠蛾在内的各种水产品中的 22 种兽药残留,包括 12 种磺胺类药物(SA)和 10 种喹诺酮类药物(QN)。为了进行比较分析,我们参考了 2005 年至 2020 年间中国有关在水产品中检出 SAs 和 QNs 的文献。此外,我们还计算了抗生素的食品安全指数(IFS),并进行了健康风险评估。研究结果表明,食用蝙蝠蛾中抗生素的检出率、平均残留量和超标率均高于其他大多数水产品。此外,系统聚类分析表明,食用蝙蝠蛾可作为潜在的哨点动物,区别于其他几种水产品。在本次研究中,从不同水产品中获得的所有残留物的总 IFS 均小于 1,这表明它们对公众健康是安全的。不过,对于某些抗生素在特定水产品中的最大残留量超出安全限值的情况,有必要进行详细调查,以了解其对人类健康的影响。
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引用次数: 0
A 28-day subacute oral toxicity study of Apis cerana (Fabricius) honey in Wistar rats 陶瓷蜂(Fabricius)蜂蜜对 Wistar 大鼠为期 28 天的亚急性毒性口服研究
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.11
Hongju Du, Peng Zhang, Shan Zheng, Yanmin Nie, Wenjing Zhang, Ying Feng, Jun-yu Ning, Guojun Li, Shan Gao
The use of honey as food and medicine is widespread, but insufficient data support that it is safe, especially when consumed in high doses. As a result, the present work aimed to investigate the potential toxicity using a repeated dose oral toxicity study. In the toxicity study, Wistar rats were divided into five groups, and orally administered with distilled water (control), 3, 6, 12, and 24 g/kg body weight (BW)/day of honey for 28 days in a row. Body weight, food consumption, clinical pathology, and histopathology were then examined. Significant suppression of body weight, food consumption, and body weight gain was observed at the dose of 24 g/kg BW in both sexes. Honey administration had no statistically significant effect on any of the haematological parameters. The clinical observations, blood coagulation and biochemical parameters, target organs, or histopathology did not reveal any additional nor other treatment-related adverse effects. Mild pathological changes in hepatic tissues were observed in the control, 12, or 24 g/kg BW dose groups, which were common spontaneous lesions unrelated to honey treatment. In the 24 g/kg BW group, one male rat showed non-specific reactions such as focal basophilic change of renal tubule cells, which were also regarded as spontaneous lesions. Based on these results, the no-observed-adverse-effect level (NOAEL) of honey in this repeat dose oral toxicity study was determined to be 12 g/kg BW in both sexes of Wistar rats.
蜂蜜被广泛用作食品和药物,但没有足够的数据证明它是安全的,尤其是在大剂量食用时。因此,本研究旨在通过重复剂量口服毒性研究来调查蜂蜜的潜在毒性。在毒性研究中,Wistar 大鼠被分为五组,连续 28 天分别口服蒸馏水(对照组)、3、6、12 和 24 克/千克体重(BW)/天的蜂蜜。然后对体重、食量、临床病理学和组织病理学进行检查。在每公斤体重 24 克的剂量下,雌雄大黄蜂的体重、食量和体重增加均受到显著抑制。在统计学上,服用蜂蜜对任何血液学参数都没有显著影响。临床观察、血液凝固和生化参数、靶器官或组织病理学均未发现任何其他与治疗相关的不良反应。对照组、12 克/千克体重组和 24 克/千克体重组的肝组织出现了轻微的病理变化,这是常见的自发性病变,与蜂蜜治疗无关。在每千克体重 24 克剂量组中,一只雄性大鼠出现了非特异性反应,如肾小管细胞局灶性嗜碱性改变,这也被视为自发性病变。根据上述结果,在这项重复剂量口服毒性研究中,确定蜂蜜对 Wistar 大鼠的无观测不良效应水平为每公斤体重 12 克。
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引用次数: 0
Parboiling of pigmented and non-pigmented Philippine rice (Oryza sativa L.) cultivars: Textural properties and carbohydrate quality 色素和非色素菲律宾大米(Oryza sativa L.)栽培品种的煮沸:纹理特性和碳水化合物质量
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.21
J. E. I. Zapater, R. Manaois
Parboiling improves rice carbohydrate quality, which is beneficial for diabetes management in Asia. However, parboiling drastically alters cooked rice texture, making it unappealing for Filipinos, who generally prefer tender cooked rice. The present work evaluated the carbohydrate quality and cooked rice hardness of nine local pigmented and non-pigmented rice varieties as affected by parboiling and cooking treatments. The amylose content (AC), retrogradation potential (RP), and resistant starch (RS) content were determined, and the samples with the highest RS were further investigated for their thermal properties and starch morphology. Results showed that parboiling increased RS level by as much as five-fold, particularly in the red rice Minaangan, which had intermediate AC and the highest RP (2,821.5 cP) among the samples. The highest RS, 2.71 ± 0.02 g/100 g, was recorded in parboiled NSIC Rc 222 cooked at 1:2.5 (rice:water). It also produced soft cooked rice, which was significantly better than its non-parboiled counterpart. High proportion of water needed to gelatinise parboiled non-pigmented NSIC Rc 222 was due to higher starch crystallinity as indicated by its gelatinisation enthalpy and intact starch granule. In conclusion, parboiling and cooking at appropriate rice:water ratio of NSIC Rc222 and Minaangan could deliver healthier cooked rice with Filipino preferred textural quality.
煮沸可提高大米碳水化合物的质量,在亚洲有利于糖尿病的控制。然而,沸煮会极大地改变大米的口感,使大米变得不好吃,因为菲律宾人通常更喜欢口感细嫩的大米。本研究评估了九个本地色素和非色素大米品种的碳水化合物质量和熟米硬度受煮沸和烹饪处理的影响。测定了淀粉含量(AC)、逆变潜能值(RP)和抗性淀粉(RS)含量,并进一步研究了抗性淀粉含量最高的样品的热特性和淀粉形态。结果表明,煮沸可使抗性淀粉含量增加五倍之多,尤其是红米米南干,其抗性淀粉含量在所有样品中居中,抗性淀粉降解潜能值(RP)最高(2,821.5 cP)。以 1:2.5 的比例(米:水)煮熟的国家科学与工业研究中心 Rc 222 的 RS 值最高,为 2.71 ± 0.02 克/100 克。煮出的米饭也更软,明显优于未经沸煮的同类产品。从糊化焓和完整的淀粉颗粒来看,沸煮非色素糙米 Rc 222 的淀粉结晶度较高,因此糊化糙米 Rc 222 所需的水比例较高。总之,以适当的米:水比例沸煮和烹饪新国标 Rc222 和 Minaangan 可提供更健康的熟米饭,并具有菲律宾人喜欢的口感质量。
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引用次数: 0
Preparation of chickpea peptide-calcium chelates with antioxidant activity 制备具有抗氧化活性的鹰嘴豆肽钙螯合物
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.10
Wen Shi, Chuangyu Tan
Chelation of antioxidant chickpea peptides with calcium can improve the absorption rate of calcium in human intestinal tract. In the present work, the optimum enzymatic hydrolysis scheme was determined by a single-factor test with an orthogonal experimental design with the following conditions: material-liquid ratio, 1:15; enzymatic hydrolysis time, 10 min; enzyme-substrate ratio, 1:20 (papain protease); and enzymatic hydrolysis temperature, 60°C. The protein hydrolysate obtained under these conditions had high antioxidant activity. The free radical scavenging rates of •OH, O2-•, DPPH•, and H2O2 were 49.44, 63.64, 66.57, and 57.64%, respectively. The reducing power was 0.75. The optimum conditions for the preparation of calcium chelate from chickpea peptides were determined by an orthogonal optimisation test: the peptide-calcium ratio, 7:1; the chelation temperature, 50°C; the initial pH of the reaction solution, 7.0; the chelation time, 40 min; and the chelation rate, 39.95%. Antioxidant chickpea peptide-calcium chelates could have the potential to induce synergistic physiological effects.
抗氧化鹰嘴豆肽与钙的螯合作用可提高人体肠道对钙的吸收率。本研究采用正交实验设计,通过单因素试验确定了最佳酶水解方案,条件如下:料液比 1:15;酶水解时间 10 分钟;酶与底物比 1:20(木瓜蛋白酶);酶水解温度 60°C。在这些条件下得到的蛋白质水解物具有很高的抗氧化活性。对-OH、O2-、DPPH-和H2O2的自由基清除率分别为49.44%、63.64%、66.57%和57.64%。还原力为 0.75。通过正交优化试验确定了鹰嘴豆肽制备螯合钙的最佳条件:肽钙比为 7:1;螯合温度为 50°C;反应液初始 pH 为 7.0;螯合时间为 40 分钟;螯合率为 39.95%。抗氧化鹰嘴豆肽钙螯合物有可能产生协同生理效应。
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引用次数: 0
Quality assessment of promising garlic (Allium sativum L.) varieties based on principal component analysis 基于主成分分析的有前途大蒜(Allium sativum L.)品种的质量评估
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.16
Prem Prakash, Kamlesh Prakash
Physicochemical properties, pungency, anti-nutritional factors, cutting strength, and colour attributes of seven promising garlic varieties were assessed for their potential application. The garlic varieties were found to be slightly acidic (pH 6.08 - 6.77), and contained varying amounts of crude protein, carbohydrate, crude fat, ash, crude fibre, acidity, and total soluble solids (TSS). The pungency factor as pyruvic acid was found to be in the range of 34.84 - 86.69 μmol/mL, whereas anti-nutritional factors such as saponin (6.63 - 13.98 g), phytic acid (0.03 - 0.6 g), and tannin (0.18 - 0.39 gCE) per 100 g on a fresh weight basis were present. The Bhima purple (BP) variety with a purple tinge showed higher saponin content than the other varieties with a whitish tinge. The cutting strength of garlic cloves varied between 17.20 and 104.61 N. Physical and gravimetric properties were estimated and found to be variety-dependent (p ≤ 0.05). Colour attributes like L* (lightness), a* (redness), b* (yellowness), chroma, hue, and browning index were significantly different (p ≤ 0.05) for all the garlic varieties. The minimum hue value, and maximum a* and b* values, browning index (BI), and chroma were found in the BP variety. Principal component analysis as a significant multivariate analysis tool was applied to assess the existence of correlation among the estimated parameters. It was found to be 75.41% of the total variance when considering the first three principals. Haryana garlic-17 (HG-17) variety showed significant quality as it yielded positive correlation with the maximum number of quality attributes.
评估了七个有潜力的大蒜品种的理化特性、辛辣度、抗营养因子、切割强度和颜色属性,以确定其潜在的应用价值。结果发现,这些大蒜品种呈微酸性(pH 值为 6.08 - 6.77),含有不同数量的粗蛋白、碳水化合物、粗脂肪、灰分、粗纤维、酸度和总可溶性固形物(TSS)。辛辣系数(丙酮酸)的范围为 34.84 - 86.69 μmol/mL,而抗营养系数(皂苷)(6.63 - 13.98 g)、植酸(0.03 - 0.6 g)和单宁(0.18 - 0.39 gCE)(以鲜重为基础)则为每 100 g。紫红色的比马紫(BP)品种的皂苷含量高于其他带白色的品种。蒜瓣的切割力介于 17.20 和 104.61 牛顿之间,物理和重量特性经估算后发现与品种有关(p ≤ 0.05)。所有大蒜品种的颜色属性,如 L*(亮度)、a*(红度)、b*(黄度)、色度、色调和褐变指数都有显著差异(p ≤ 0.05)。BP 品种的色调值最小,a*和 b*值、褐变指数(BI)和色度最大。主成分分析是一种重要的多元分析工具,用于评估估计参数之间是否存在相关性。在考虑前三个主成分时,发现其占总方差的 75.41%。哈里亚纳大蒜-17(HG-17)品种显示出显著的品质,因为它与最多的品质属性呈正相关。
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引用次数: 0
Modified atmosphere packaging of flounder fillet: Modelling of package conditions and comparison of different flushing atmospheres for quality preservation 比目鱼片的气调包装:包装条件建模和比较不同冲洗气氛的保质效果
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.14
Eo Jin Park, Soo Yeon Jung, Su Chan Kim, Dong Sun Lee, D. An
Modified atmosphere packaging (MAP) flushed in high CO2 concentration with high gas barrier layer is often applied to preserve fish at chilled temperatures. Dissolution of CO2 in fish product results in changes from the original CO2 concentration and volume of packages. Maintenance of the desired CO2 concentration under appropriate package volume is required for realising effectiveness in fish preservation and handling through distribution channel. In the present work, mathematical model for estimating package gas compositional changes along with volume decrease was developed by incorporating CO2 dissolution dynamics into gas mass balance equation, and it was then validated by 200 g flounder packages flushed with atmospheres of 30 or 60% CO2 and stored at 5°C. Then, the effect of different modified atmospheres (MAs) on preservation of the flounder fillets was examined for 100 g flounder packages stored at 10°C for 5 d. Package flushed with MA of CO2 60%/O2 30%/N2 10% was the best for preserving flounder fillet preservation, in terms of total aerobic bacteria inhibition and low volatile basic nitrogen content as major quality indexes.
带有高阻气层的高浓度二氧化碳气调包装(MAP)通常用于在冷藏温度下保存鱼类。二氧化碳在鱼产品中的溶解会导致包装中的二氧化碳浓度和体积发生变化。要在适当的包装体积下保持所需的二氧化碳浓度,才能实现鱼类保存的有效性,并通过分销渠道进行处理。在本研究中,通过将二氧化碳溶解动力学纳入气体质量平衡方程,建立了估算包装气体成分随体积减小而变化的数学模型,并通过 200 克比目鱼包装在 30% 或 60% 二氧化碳气氛中冲洗并在 5°C 下储存进行了验证。从抑制需氧菌总数和低挥发性碱基氮含量这两项主要质量指标来看,冲入 CO2 60%/O2 30%/N2 10% 的改良气氛对保存比目鱼片的效果最好。
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引用次数: 0
Development of functional nougat without diabetic effects in vivo 开发体内无糖尿病影响的功能性牛轧糖
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.04
A. Ottombrino, F. Maurano, Ersilia Montesano, Adelia Picariello, Annachiara Oliviero, Mauro Rossi
Nougat is a confectionery foodstuff made of whipped egg white, sugar, honey, and nuts. In a traditional Italian nougat recipe, the mixture is flavoured with vanilla extract, and packed with toasted almonds. Traditional nougat is banned from the diets of diabetics due to high amount of sugar. In the present work, we aimed to develop innovative sugar-free nougat, and compare its textural and sensory properties with those of commercial nougat. Finally, we tested the residual hyperglycaemic effect in non-obese diabetic (NOD) mice, a model of type 1 diabetes. We developed a sugar-free nougat recipe by mixing xanthan gum, erythritol, and inulin with egg white and almonds using conventional industrial instruments. Technical analysis indicated that the structure, in terms of shear force, was comparable with that of traditional chewy nougat. Sensory analysis indicated that flavour and sweetness were preserved, whereas cohesiveness and fracturability changed significantly. Interestingly, the innovative food composition positively influenced two other texture parameters; solubility and adhesiveness. In vivo experiments showed that the number of mice in the group that was fed with experimental nougat that did not develop diabetes was significantly higher than that in the group fed with commercial nougat (66.6% vs. 33.0%; p < 0.05), and not different from that in the group fed without nougat (72.7%; p = 0.37). In conclusion, we produced, on a pilot scale, innovative sugar-free nougat with improved texture and similar sensory properties, in comparison with the traditional product. In vivo, the experimental nougat did not increase the diabetes incidence significantly.
牛轧糖是一种由打发的蛋白、糖、蜂蜜和坚果制成的糖果食品。在传统的意大利牛轧糖配方中,混合物会加入香草精和烤杏仁。传统牛轧糖因含糖量高而被糖尿病患者禁止食用。在本研究中,我们旨在开发创新的无糖牛轧糖,并将其质地和感官特性与商业牛轧糖进行比较。最后,我们还在非肥胖糖尿病(NOD)小鼠(一种 1 型糖尿病模型)身上测试了其残留的高血糖效应。我们使用传统的工业仪器,将黄原胶、赤藓糖醇和菊粉与蛋白和杏仁混合,开发出一种无糖牛轧糖配方。技术分析表明,就剪切力而言,牛轧糖的结构与传统的咀嚼牛轧糖相当。感官分析表明,风味和甜味得到了保留,而内聚力和可碎性则发生了显著变化。有趣的是,创新食品成分还对其他两个质地参数产生了积极影响:可溶性和粘合性。体内实验表明,喂食实验牛轧糖的组别中,未患糖尿病的小鼠数量明显高于喂食商业牛轧糖的组别(66.6% vs. 33.0%;p < 0.05),与未喂食牛轧糖的组别(72.7%;p = 0.37)没有差异。总之,与传统产品相比,我们试制的创新型无糖牛轧糖口感更好,感官特性相似。在体内,实验性牛轧糖没有明显增加糖尿病的发病率。
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引用次数: 0
Evaluation of food safety on traditional Chinese snacks based on system safety theory: The case study of Wuhan hot-dry noodles 基于系统安全理论的中国传统小吃食品安全评价:武汉热干面案例研究
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.03
Wei Gao, Lin Li, Yingchun Xue
Wuhan hot-dry noodles, as one of the traditional Chinese snacks, have been famous in most parts of China for nearly 100 years. Its characteristic, taste, and quick cooking method cater to the needs of many people. However, food safety has become an essential obstacle in developing Wuhan hot-dry noodles. The present work developed evaluation indicators, and selected an entropy method to analyse the food safety problems of Wuhan hot-dry noodles based on the system safety theory. Results showed that the score was 2.59 over 5.00, which meant the performance was below average. This could have been due to poor perception of employees on food safety, primary enterprise management, and less effective and authoritative standards within the industry. Based on the system theory, the present work suggested engineering, education, and enforcement, namely the "3E principle”, to solve the above problems. The key to the "3E principle” is to focus on identifying, controlling, and evaluating hazards around the most critical issues, and solving them in order of importance and urgency. The "3E principle” also emphasises circular processing for better food safety.
武汉热干面作为中国传统小吃之一,在中国大部分地区闻名遐迩已有近百年的历史。它的特色、口味和快捷的烹饪方法满足了许多人的需求。然而,食品安全已成为武汉热干面发展过程中的一个重要障碍。本研究以系统安全理论为基础,制定了评价指标,并选用熵值法对武汉热干面的食品安全问题进行了分析。结果表明,评分为 2.59,高于 5.00,即表现低于平均水平。这可能是由于员工的食品安全意识薄弱、企业管理初级、行业内有效和权威的标准较少等原因造成的。基于系统理论,本研究提出了工程、教育和执行,即 "3E 原则 "来解决上述问题。3E 原则 "的关键在于围绕最关键的问题,重点识别、控制和评估危害,并按照重要性和紧迫性的顺序加以解决。3E 原则 "还强调循环加工,以提高食品安全。
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引用次数: 0
Sucrose reduction and addition of agave syrup and inulin in gummies with strawberry and blackberry pulp: Impact on physicochemical, antioxidant, and sensory characteristics 在草莓和黑莓果肉软糖中减少蔗糖含量并添加龙舌兰糖浆和菊粉:对理化、抗氧化和感官特性的影响
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.18
C. López-Palestina, Ángeles García-García, Susana Elizabeth Altamirano Romo, Jorge Gutiérrez-Tlahque
The current trend in the food industry involves the search for new formulations and processes to produce processed foods with enhanced nutritional value and improved functional properties that promote consumer health. Therefore, the present work aimed to investigate the physicochemical, antioxidant, and sensory properties of gummies made from strawberry and blackberry pulp. These gummies were formulated with reduced sucrose content and supplemented with agave syrup and inulin. Six formulations were developed using three levels of sucrose reduction (SR) namely 0, 40, and 50%, combined with two types of natural fruit pulp: strawberry (S) and blackberry (B). For the formulations with 40 and 50% SR, agave syrup and inulin were incorporated at a 1:1 ratio. The evaluation encompassed nutritional content, antioxidant activity, textural properties, and the overall acceptability of the various gummy products. Results demonstrated that SR led to a decrease in the caloric content of the gummies, from 279.38 to 178.69 kcal per 100 g. Gummies containing a blend of inulin and agave syrup exhibited an average of 3.69 times higher dietary fibre content. Moreover, the antioxidant compounds inherent in strawberry and blackberry pulp were effectively preserved during processing. Samples with 50% SR displayed an increase of up to 24.80 and 34.89% in phenolic and flavonoid contents, respectively, as compared to those with 0% SR. These samples also demonstrated elevated antioxidant activity as assessed by the ABTS and DPPH assays, reaching up to 109.4 µM Trolox per 100 g, and an inhibition rate of 77.9%, respectively. However, the incorporation of SR in the formulations had a noticeable impact on the textural properties of the gummies, particularly in terms of gumminess and chewiness. In terms of sensory analysis, it was observed that gummies labelled as S-SR-50 and B-SR-50 exhibited higher levels of acceptability, primarily attributed to their enhanced aroma, colour, and taste qualities. The findings of the present work propose a promising avenue for gummy candy reformulation involving reduced sucrose content and the reintroduction of inulin, agave syrup, and the inherent natural antioxidants from strawberry and blackberry. This approach could be a feasible strategy to produce gummy confections with improved nutritional values and antioxidant properties.
食品工业当前的趋势是寻找新配方和新工艺,以生产具有更高的营养价值和更好的功能特性的加工食品,从而促进消费者的健康。因此,本研究旨在调查用草莓和黑莓果肉制成的软糖的理化、抗氧化和感官特性。这些软糖的配方减少了蔗糖含量,并添加了龙舌兰糖浆和菊粉。使用三种蔗糖减量(SR)水平,即 0、40 和 50%,结合草莓(S)和黑莓(B)两种天然果肉,开发了六种配方。在蔗糖减量为 40% 和 50% 的配方中,龙舌兰糖浆和菊粉的比例为 1:1。评估内容包括各种软糖产品的营养成分、抗氧化活性、质地特性和整体可接受性。结果表明,SR 使软糖的热量从每 100 克 279.38 千卡降至 178.69 千卡。此外,草莓和黑莓果肉中固有的抗氧化化合物在加工过程中得到了有效保存。酚类和类黄酮含量为 50%的样品比 0%的样品分别增加了 24.80% 和 34.89%。根据 ABTS 和 DPPH 试验的评估,这些样品的抗氧化活性也有所提高,每 100 克样品中的 Trolox 含量高达 109.4 µM,抑制率为 77.9%。不过,在配方中加入 SR 对软糖的质地特性有明显的影响,特别是在胶质感和咀嚼感方面。在感官分析方面,观察到标有 S-SR-50 和 B-SR-50 的软糖表现出更高的可接受性,这主要归功于它们的香气、颜色和口感品质得到了提升。本研究的结果为软糖的重新配方提出了一个很有前景的途径,即减少蔗糖含量,重新添加菊粉、龙舌兰糖浆以及草莓和黑莓中固有的天然抗氧化剂。这种方法是一种可行的策略,可以生产出营养价值更高、抗氧化性更强的软糖。
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引用次数: 0
Symbiosis of acetic acid bacteria and yeast isolated from black tea fungus mimicking the kombucha environment in bacterial cellulose synthesis 从模仿昆布茶环境的红茶真菌中分离出的醋酸菌和酵母菌在细菌纤维素合成中的共生关系
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-12-20 DOI: 10.47836/ifrj.30.6.13
Xiaoyuan Li, Zhizhou Chen, Jiechen Wang, Jianlou Mu, Qianyun Ma, Xiaomeng Lu
The symbiotic effect of acetic acid bacteria and yeast on bacterial cellulose (BC) synthesis in kombucha was explored. Firstly, the optimal culture ratio of acetic acid bacteria and yeast was optimised through single factor and orthogonal test. The results showed that when Komagataeibacter intermedius:Brettanomyces bruxellensis:Zygosaccharomyces bisporus ratio was 1:10:10, and the inoculation amounts of K. intermedius, B. bruxellensis, and Z. bisporus were 104, 105, and 105 CFU/mL, respectively, the yield of BC was the highest, and the dry basis was 5.51 g/L. It was determined that the metabolites of B. bruxellensis and Z. bisporus could promote the synthesis of BC by K. intermedius. In addition, the composition of yeast filtrate was analysed by amino acid analyser, gas chromatography-mass spectrometry (GC-MS), and high performance liquid chromatograph (HPLC). Results showed that 16 amino acids were detected in yeast filtrate, and cysteine was only detected in yeast filtrate. The increase in isoleucine before and after fermentation was the highest, which was 11.64 times that of the control group. The increase in aspartic acid and glycine were second and third, accounting for 60.00 and 41.67%, respectively. The main volatile substances were alcohols, accounting for 84.89%, of which the relative content of ethanol was the highest at 77.35%. The relative contents of 3-methyl-1-butanol and phenylethanol were also high, accounting for 4.13 and 3.14%, respectively. Tartaric, citric, and succinic acids were detected in the yeast filtrate. The chemical species did not change before and after fermentation, but the content decreased. Vitamins B2 and B6 were detected in yeast filtrate, and the species and content did not change significantly before and after fermentation. A theoretical basis for kombucha fermentation and BC synthesis was provided.
本研究探讨了醋酸菌和酵母菌对昆布茶中细菌纤维素(BC)合成的共生效应。首先,通过单因子和正交试验优化了醋酸菌和酵母菌的最佳培养比例。结果表明,当中间酵母菌(Komagataeibacter intermedius):布鲁氏酵母菌(Brettanomyces bruxellensis):双孢酵母菌(Zygosaccharomyces bisporus)的比例为 1:10:10,中间酵母菌(K. intermedius)、布鲁氏酵母菌(B. bruxellensis)和双孢酵母菌(Z. bisporus)的接种量分别为 104、105 和 105 CFU/mL 时,BC 的产量最高,干基为 5.51 g/L。结果表明,布鲁氏菌和双孢蘑菇的代谢产物可促进中间体合成 BC。此外,氨基酸分析仪、气相色谱-质谱联用仪(GC-MS)和高效液相色谱仪(HPLC)分析了酵母滤液的成分。结果表明,在酵母滤液中检测到 16 种氨基酸,仅在酵母滤液中检测到半胱氨酸。发酵前后异亮氨酸的增幅最大,是对照组的 11.64 倍。天门冬氨酸和甘氨酸的增幅位居第二和第三,分别占 60.00% 和 41.67%。主要挥发性物质是醇类,占 84.89%,其中乙醇的相对含量最高,为 77.35%。3 甲基-1-丁醇和苯乙醇的相对含量也很高,分别占 4.13% 和 3.14%。酵母滤液中检测到酒石酸、柠檬酸和琥珀酸。化学种类在发酵前后没有变化,但含量有所下降。在酵母滤液中检测到了维生素 B2 和 B6,其种类和含量在发酵前后没有明显变化。这为昆布茶发酵和萃取物合成提供了理论依据。
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international food research journal
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