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Effects of reactive extrusion on cross-linking of corn starch 反应挤压对玉米淀粉交联的影响
4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-08-30 DOI: 10.47836/ifrj.30.4.20
Maiara Pereira Mendes, Ghiovani Zanzotti Raniero, Ivo Demiate, Antonio Roberto Giriboni Monteiro
The present work aimed to evaluate the efficiency of the dual modification of corn starch by reactive extrusion, using a mono-thread extruder, and also sodium tripolyphosphate and trimetaphosphate to obtain cross-linked starch. Native starch, extruded native starch, and starch cross-linked by conventional methods were used as standards to evaluate the modification efficacy by reactive extrusion. The content of phosphorus residues allowed by legislation in starch modification is 0.4%. Therefore, the reactive extrusion demonstrated greater effectiveness because cross-linked starch obtained by this method was within the established limit of phosphorus residues, in contrast to that obtained by the conventional method, which exceeded this amount. In addition, it required greater water consumption for residual removal, therefore producing effluents, and also wasting energy and time. Cross-linking by the extrusion process showed a profound influence on the characteristics of modified starch, generating specific functional characteristics, mainly in terms of pasting properties. Also, the samples presented low viscosity and pasting temperature. In conclusion, cross-linking through reactive extrusion was fast, produced starch with phosphorus content within the limits allowed by legislation, and generated different physicochemical characteristics.
研究了用单丝挤出机和三聚磷酸钠、三偏磷酸钠对玉米淀粉进行双重改性,得到交联淀粉的效果。以天然淀粉、挤出的天然淀粉和常规交联的淀粉为标准,评价反应挤出改性的效果。法定淀粉改性中磷残留量为0.4%。因此,反应挤压显示出更大的有效性,因为用这种方法得到的交联淀粉在磷残留量的规定范围内,而传统方法得到的磷残留量超过了这个限度。此外,它需要消耗更多的水来去除残留物,因此产生废水,也浪费了能源和时间。挤压交联对变性淀粉的特性产生了深远的影响,产生了特定的功能特性,主要表现在糊化性能方面。样品具有较低的黏度和黏度。综上所述,反应挤出交联速度快,制备的淀粉含磷量在法律允许的范围内,并产生不同的理化特性。
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引用次数: 0
Fabrication of polyvinyl alcohol-starch controlled release active film incorporated with 2-hydroxypropyl-β-cyclodextrin/lemongrass oil emulsion for large yellow croaker (Pseudosciaena crocea) preservation 2-羟丙基-β-环糊精/香茅油乳剂聚乙烯醇-淀粉控释活性膜的制备及大黄鱼保鲜用研究
4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-08-30 DOI: 10.47836/ifrj.30.4.08
Jiaxi Wang, Xinyi Fan, Zhijie Chen, Chenwei Chen, Jing Xie
Polyvinyl alcohol-starch (PVA/ST) active films incorporated with lemongrass oil (LMO) or 2-hydroxypropyl-β-cyclodextrin, and LMO (HP-β-CD/LMO) emulsion were developed in the present work. The effects of LMO or HP-β-CD/LMO emulsion on the properties of films, and their application in large yellow croaker preservation were investigated. The average particle size and the encapsulation efficiency of the HP-β-CD/LMO emulsion were 150.07 nm and 81.32%, respectively. The scanning electron microscopy (SEM) results revealed that HP-β-CD improved the compatibility between PVA and starch, and LMO was well embedded in HP-β-CD. The incorporation of LMO or HP-β-CD/LMO enhanced the water vapour barrier property and flexibility of the film while weakening its mechanical strength. The oxygen barrier property of the film was weakened by the incorporation of LMO and strengthened by HP-β-CD/LMO. The film incorporated with HP-β-CD/LMO exhibited a little weaker antioxidant and antibacterial activities than the film containing LMO owing to their release property. The existence of HP-β-CD postponed the release of LMO from the film into food simulant (10% ethanol). The preservation results demonstrated that the film containing LMO or HP-β-CD/LMO efficiently inhibited the growth of microorganisms and lipid oxidation of fish; and delayed the decomposition of protein and freshness reduction of large yellow croaker. Additionally, the film added with HP-β-CD/LMO exhibited the best protection for fish quality. In other words, the film with the proper release property of active agents contributed to the preservation of aquatic products.
以香茅油(LMO)或2-羟丙基-β-环糊精为原料,制备了聚乙烯醇-淀粉(PVA/ST)活性膜和LMO (HP-β-CD/LMO)乳液。研究了LMO或HP-β-CD/LMO乳剂对大黄鱼保鲜膜性能的影响及其在大黄鱼保鲜膜中的应用。HP-β-CD/LMO乳液的平均粒径为150.07 nm,包封率为81.32%。扫描电镜(SEM)结果表明,HP-β-CD改善了PVA与淀粉的相容性,LMO在HP-β-CD中包埋良好。LMO或HP-β-CD/LMO的掺入提高了膜的水蒸气阻隔性能和柔韧性,但降低了膜的机械强度。LMO的加入削弱了膜的阻氧性能,HP-β-CD/LMO的加入增强了膜的阻氧性能。由于HP-β-CD/LMO的释放特性,掺入HP-β-CD/LMO的膜的抗氧化和抗菌活性略弱于含LMO的膜。HP-β-CD的存在延缓了LMO从薄膜向食品模拟剂(10%乙醇)的释放。保存结果表明,含有LMO或HP-β-CD/LMO的膜有效地抑制了微生物的生长和鱼类的脂质氧化;延缓了大黄鱼蛋白质的分解和新鲜度的降低。此外,添加HP-β-CD/LMO的膜对鱼品质的保护效果最好。换句话说,具有适当的活性剂释放特性的薄膜有助于水产品的保存。
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引用次数: 0
Antimicrobial peptides: Design, chemical synthesis, activity evaluation, and application 抗菌肽:设计、化学合成、活性评价和应用
4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-08-30 DOI: 10.47836/ifrj.30.4.01
Hongyan Yang, Lan Wang, Heng Du, Dongxin Zhao, Chuang Liu, Kui Lu
Antimicrobial peptides (AMPs) are active short peptides that exist in microorganisms, insects, amphibians, plants, and mammals. Some naturally occurring AMPs have low antimicrobial activity, high haemolysis, potential toxicity toward mammalian cells, and high susceptibility to proteolytic degradation, which limit their practical application. In recent years, many efforts have been made to design and modify AMPs to improve their properties. The present review focuses on site-directed mutation, truncation, hybridisation, capping, and cyclisation of AMPs. The review further introduces the application of solid-phase peptide synthesis technology for AMPs, and summarises the methods for evaluating the antimicrobial activity of AMPs. The in-depth research on AMPs is expected to play an essential role in the fields of agriculture, animal husbandry, food industry, and medicine.
抗菌肽是存在于微生物、昆虫、两栖动物、植物和哺乳动物体内的活性短肽。一些天然存在的抗菌肽具有抗菌活性低、溶血能力强、对哺乳动物细胞有潜在毒性和对蛋白水解降解的高易感性等特点,限制了其实际应用。近年来,人们在设计和改造amp方面做出了许多努力,以改善其性能。目前的综述集中在定点突变,截断,杂交,封顶和amp的环化。综述了固相肽合成技术在抗菌肽合成中的应用,并对抗菌肽抗菌活性的评价方法进行了综述。对抗菌肽的深入研究有望在农业、畜牧业、食品工业和医药等领域发挥重要作用。
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引用次数: 0
Physical properties and glycaemic response of tapioca noodles fortified with herb mixture 草药混合物强化木薯粉的物理特性和血糖反应
4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-08-30 DOI: 10.47836/ifrj.30.4.18
Samsu Udayana Nurdin, Ratna Wylis Arief, Banon Rustiaty, Robet Asnawi, Amalia M. Mar’atun, None Arfiathi, Dyah Koesoemawardani, Neti Yuliana, Sutopo Hadi
High noodle consumption is associated with an increased risk of generative diseases due to noodles' relatively high glycaemic response. Plants containing high phenolic compounds can reduce the glycaemic response of carbohydrate-rich foods. The present work thus aimed to evaluate the physical properties and glycaemic response of tapioca noodles fortified with a mixture of turmeric (T), cinnamon (C), and guava leaves (G) powder. The proportions of herbs (T: C: G) were 0.00: 0.00: 0.00 g (C1); 1.00: 0.50: 1.50 g (C2); 1.33: 0.67: 1.00 g (C3); 1.67: 0.83: 0.50 g (C4); and 2.00: 1.00: 0.00 g (C5). Herb mixtures increased the total phenolic and flavonoid contents of the noodles. Noodles with higher tensile strength (TS) were harder and less swollen because the water absorption capacity (WAC) was lower, but effect of the formulas on the oil absorption capacity (OAC) varied. Area under the curve (AUC) of blood sugar levels showed that C2 noodles had the smallest area (2,321 units) as compared to C1 (3,066 units) and C5 (3,241 units) noodles, but the difference was not statistically significant, thus indicating that the glycaemic responses of these noodles were similar to that of the original noodles when consumed by healthy volunteers.
由于面条的血糖反应相对较高,大量食用面条与生殖疾病的风险增加有关。含有高酚类化合物的植物可以减少富含碳水化合物的食物的血糖反应。因此,本研究旨在评估用姜黄(T)、肉桂(C)和番石榴叶(G)粉混合强化的木薯粉的物理特性和血糖反应。药材配比(T: C: G)为0.00:0.00:0.00 G (C1);1.00: 0.50: 1.50 g (C2);1.33: 0.67: 1.00 g (C3);1.67: 0.83: 0.50 g (C4);和2.00:1.00:0.00 g (C5)。混合草药可提高面条中总酚和类黄酮含量。抗拉强度高的面条由于吸水率低而较硬,肿胀程度低,但配方对吸油率的影响不同。血糖水平曲线下面积(AUC)显示,C2面条面积最小(2321单位),C1面条面积最小(3066单位),C5面条面积最小(3241单位),但差异无统计学意义,这表明健康志愿者食用这些面条时的血糖反应与原始面条相似。
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引用次数: 0
Chitosan and salicylic acid as alternatives for the control of postharvest fungal diseases in blueberries (Vaccinium corymbosum) 壳聚糖和水杨酸防治蓝莓采后真菌病的研究
4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-08-30 DOI: 10.47836/ifrj.30.4.16
Surelys Ramos-Bell, Luis Guillermo Hernandez-Montiel, Rita Velázquez Estrada, Cristina Moreno-Hernández, Porfirio Gutierrez-Martinez
Blueberry (Vaccinium corymbosum) has characteristics that make it a highly coveted fruit by the population that seeks benefits for their health, thus giving it economic and social relevance. However, it is a very perishable fruit. In the present work, Botrytis sp., Penicillium sp., and Alternaria sp. were isolated from blueberry, and molecularly identified. The in vitro effect of chitosan (CHI) and salicylic acid (SA) on the growth of these phytopathogens was then evaluated, as well as the incidence of the disease after the application of these treatments on blueberry. CHI at 1.5% achieved an in vitro mycelial growth inhibition of Botrytis sp., Penicillium sp., and Alternaria sp. by 93, 84, and 40%, respectively. Furthermore, a complete germination inhibition of Penicillium sp. and Alternaria sp. was accomplished; Botrytis sp. spores were less sensitive to chitosan treatment. The germination percentage of the phytopathogens was reduced by 90% using SA at 5 mM. The in vivo application of CHI at 1.5% and SA at 5 mM decreased the percentage of incidence of phytopathogens in blueberries harvested after storage period at 25°C, as compared to the control. Based on these results, SA and CHI represent an alternative for the control of phytopathogens in blueberry to eliminate the use of synthetic fungicides.
蓝莓(Vaccinium corymbosum)的特点使其成为寻求健康益处的人群非常垂涎的水果,从而具有经济和社会意义。然而,它是一种很容易腐烂的水果。本文从蓝莓中分离得到了葡萄孢菌、青霉菌和交替孢菌,并进行了分子鉴定。然后评价壳聚糖(CHI)和水杨酸(SA)对这些植物病原体生长的体外影响,以及这些处理在蓝莓上应用后的发病率。1.5%浓度的CHI对葡萄孢菌、青霉菌和交替孢菌的体外生长抑制率分别为93%、84%和40%。此外,青霉菌和互交菌的萌发被完全抑制;Botrytis sp.孢子对壳聚糖处理不敏感。与对照相比,在25°C贮藏期后收获的蓝莓中,体内施用1.5% CHI和5 mM SA降低了植物病原体的发芽率90%。综上所述,SA和CHI为蓝莓植物病原菌的防治提供了一种替代方法,以消除合成杀菌剂的使用。
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引用次数: 0
Formulation of halalan toyyiban radish effervescent tablet 哈拉兰妥益班萝卜泡腾片的研制
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-08-21 DOI: 10.47836/ifrj.30.3.08
Nur Najihah Azahar, N. Muhammad, Nur Fazira Abdul Rahim
Radish is a vegetable high in nutritional and phytochemical contents that are beneficial to the human body, especially for managing kidney stone disease. However, it is less consumed as compared to other vegetables due to its pungent flavour and odour. Therefore, the objective of the present work was to formulate a radish effervescent tablet to enhance the palatability of the vegetable. The radish effervescent tablet was formulated by using Simplex Lattice Mixture Design where the percentages of sucralose and strawberry flavour were chosen as the factors. Five formulations were generated, and sensory acceptance test was conducted on them. Next, the halalan toyyiban principle compliance was evaluated based on the Halal Control Points (HCPs), toxicity assay (brine shrimp lethality assay), anti-urolithiatic properties (turbidimetric assay), and nutritional composition (energy, total protein, total fat, total carbohydrate, and total sugar). Formulation 5 (F5) with 20% citric acid, 12% sodium bicarbonate, 58% radish powder, 2.5% sucralose, and 7.5% strawberry flavour was selected as the most acceptable formulation (p < 0.05). For the halalan toyyiban principle compliance, F5 was evaluated, and it complied with the halalan toyyiban principles. It is halal, non-toxic, and safe for consumption as the LC50 was greater than 1,000 μg/mL (2,223.31 μg/mL) for toxicity test, and exhibited significant potential as an anti-urolithiatic agent (88.13% inhibition). In the future, prototype development of radish effervescent tablet with potential anti-urolithiatic and fast-dissolving properties can be carried out.
萝卜是一种富含营养和植物化学成分的蔬菜,对人体有益,特别是对治疗肾结石疾病。然而,由于其刺鼻的味道和气味,与其他蔬菜相比,它的消费量较少。因此,本工作的目的是研制一种萝卜泡腾片,以提高萝卜的适口性。以三氯蔗糖和草莓香精的含量为影响因素,采用单形晶格混合设计制备萝卜泡腾片。生成了5种配方,并对其进行了感官验收试验。接下来,根据清真控制点(HCPs)、毒性测定(卤虾致死性测定)、抗尿石特性(浊度测定)和营养成分(能量、总蛋白质、总脂肪、总碳水化合物和总糖)对清真素toyyiban原则的依从性进行评估。选择柠檬酸20%、碳酸氢钠12%、萝卜粉58%、三氯蔗糖2.5%、草莓香精7.5%的配方5 (F5)为接受度最高的配方(p < 0.05)。对于halalan toyyban原则符合性,对F5进行了评估,F5符合halalan toyyban原则。其清真、无毒、安全,毒性试验LC50大于1000 μg/mL (223.31 μg/mL),具有显著的抗尿石作用潜力(抑制率达88.13%)。在未来,可以进行具有抗尿石和快速溶解性能的萝卜泡腾片的原型开发。
{"title":"Formulation of halalan toyyiban radish effervescent tablet","authors":"Nur Najihah Azahar, N. Muhammad, Nur Fazira Abdul Rahim","doi":"10.47836/ifrj.30.3.08","DOIUrl":"https://doi.org/10.47836/ifrj.30.3.08","url":null,"abstract":"Radish is a vegetable high in nutritional and phytochemical contents that are beneficial to the human body, especially for managing kidney stone disease. However, it is less consumed as compared to other vegetables due to its pungent flavour and odour. Therefore, the objective of the present work was to formulate a radish effervescent tablet to enhance the palatability of the vegetable. The radish effervescent tablet was formulated by using Simplex Lattice Mixture Design where the percentages of sucralose and strawberry flavour were chosen as the factors. Five formulations were generated, and sensory acceptance test was conducted on them. Next, the halalan toyyiban principle compliance was evaluated based on the Halal Control Points (HCPs), toxicity assay (brine shrimp lethality assay), anti-urolithiatic properties (turbidimetric assay), and nutritional composition (energy, total protein, total fat, total carbohydrate, and total sugar). Formulation 5 (F5) with 20% citric acid, 12% sodium bicarbonate, 58% radish powder, 2.5% sucralose, and 7.5% strawberry flavour was selected as the most acceptable formulation (p < 0.05). For the halalan toyyiban principle compliance, F5 was evaluated, and it complied with the halalan toyyiban principles. It is halal, non-toxic, and safe for consumption as the LC50 was greater than 1,000 μg/mL (2,223.31 μg/mL) for toxicity test, and exhibited significant potential as an anti-urolithiatic agent (88.13% inhibition). In the future, prototype development of radish effervescent tablet with potential anti-urolithiatic and fast-dissolving properties can be carried out.","PeriodicalId":13754,"journal":{"name":"international food research journal","volume":"20 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91169921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
UV-visible light spectra of Clitoria ternatea L. flower extract during aqueous extraction and storage 阴蒂花提取物水溶液提取和贮藏过程中的紫外-可见光光谱
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-21 DOI: 10.47836/ifrj.30.3.18
A. Marpaung, Andara Paramaputri
Spectrophotometry is a widespread method to observe anthocyanin content, colour quality, and chemical change of an anthocyanin-source extract. The spectrogram may vary due to the extraction method, and may also change over time. The present work studied the spectrophotometric varieties of butterfly pea flower (Clitoria ternatea L.) extract using the cell wall disruption method (mortar-pestle: MP; freezing-thawing: FT; 6 min steam blanching: SB; and 6 min hot water blanching: WB) and aqueous extraction temperature (30, 45, and 60°C) for 210 min. The absorbance was monitored every 30 min. The UV-vis light absorbance shift of the extract during storage at 30°C (E30) and 50°C (E50) was also evaluated. Both SB and WB resulted in extracts with a similar spectrogram and effectively suppressed the brown colour development (browning index: 0.27 ± 0.02 and 0.3 ± 0.01, respectively). The cell wall disruption method did not affect the total anthocyanin. Therefore, the blanching process before extraction was appropriate. The most effective extraction parameter yielding the highest anthocyanin and phenolic substances were 60°C for 30 min. The E50 and E30 performed differently during storage. They showed different colour degradation patterns. For the bathochromic shift, the absorbance increased at 265 nm (phenolic substances), and that at 310 nm (acyl groups) occurred in E50. These three unique characteristics might indicate the event of intermolecular co-pigmentation between or among anthocyanin molecules that led to higher anthocyanin stability at 50°C (t0.5 24.78 days) than at 30°C (t0.5 14.28 days).
分光光度法是一种广泛用于观察花青素源提取物的含量、颜色质量和化学变化的方法。谱图可能因提取方法而变化,也可能随时间而变化。采用细胞壁破坏法(臼杵法:MP;冻融:英国《金融时报》;6分钟蒸汽焯水:SB;热水烫6 min (WB)和水浸温度(30、45、60℃)210 min。每隔30 min监测吸光度。同时评估提取物在30°C (E30)和50°C (E50)下储存期间的紫外-可见光吸光度变化。褐变指数(褐变指数)分别为0.27±0.02和0.3±0.01。细胞壁破坏法对总花青素含量无明显影响。因此,提取前的焯水工艺是合适的。花青素和酚类物质提取率最高的提取参数为60°C,提取时间为30 min。E50和E30在贮藏过程中的表现不同。它们呈现出不同的颜色退化模式。对于色移,E50在265 nm处(酚类物质)吸光度增加,在310 nm处(酰基)吸光度增加。这三个独特的特征可能表明花青素分子之间或分子间的共着色事件,导致花青素在50°C (t0.5 24.78天)下的稳定性高于30°C (t0.5 14.28天)。
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引用次数: 0
Formulation and stability enhancement using vitamin A encapsulation in ocular abnormalities: A scientific review 使用维生素A包封治疗眼部异常的配方和稳定性增强:一项科学综述
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-21 DOI: 10.47836/ifrj.30.3.02
C. Chandan, H. A. Pardhe, K. Nagappan, B. Sushma, M. Jeyaprakash
The relationship between vitamin A, retinol activity, and eye health has been briefly elucidated. Based on certain reports, vitamin A and retinol activity can help overcome vitamin A insufficiency i.e., xerophthalmia. The present review assesses vitamin A sources, β-carotene and β-cryptoxanthin in vitamin A metabolism, retinol and xerophthalmia, and new application of vitamin A in mitigating xerophthalmia. Vitamin A and its precursors are sensitive, and could lose their biological activity when exposed to light or oxygen. In this context, encapsulation may act as a protection strategy for enhancing vitamin A's biological functions under adverse conditions. With the belief that vitamin A and retinol activity have a long-term association with xerophthalmia, the present review discusses the relationship between vitamin A consumption and its precursors, as well as the physiological effects of vitamin A on xerophthalmia. In conclusion, further research using free and encapsulated forms of vitamin A to prevent vitamin A deficiency and manage xerophthalmia should be emphasised.
维生素A、视黄醇活性与眼睛健康之间的关系已简要阐明。根据某些报告,维生素A和视黄醇活性可以帮助克服维生素A不足,即干眼症。本文综述了维生素A的来源、β-胡萝卜素和β-隐黄质在维生素A代谢中的作用、视黄醇与干眼症的关系以及维生素A在缓解干眼症中的新应用。维生素A和它的前体是敏感的,暴露在光或氧下可能会失去它们的生物活性。在这种情况下,胶囊化可以作为一种保护策略,在不利条件下增强维生素a的生物学功能。鉴于维生素A和视黄醇活性与干眼症有长期的联系,本文就维生素A的摄入与其前体的关系以及维生素A对干眼症的生理作用进行了探讨。总之,应强调使用游离和胶囊形式的维生素A来预防维生素A缺乏症和治疗干眼症的进一步研究。
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引用次数: 0
Green synthesis of silver nanoparticles using banana flower extract, and their antibacterial activity 利用香蕉花提取物合成绿色纳米银,并对其抗菌活性进行研究
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-21 DOI: 10.47836/ifrj.30.3.06
Weiming Gu, Quanfeng Huang, Jianxia Sun, Dan Liu, X. Duan
Silver nanoparticles (AgNPs) were synthesised using banana flower extract (BFE) as a reducing and stabilising agent. Spherical, well-dispersed, and stable AgNPs were formed and characterised by ultraviolet-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), and zeta potential. The in vitro antimicrobial properties of AgNPs against Staphylococcus aureus and Escherichia coli were then investigated. The minimum inhibitory concentration (MIC) of AgNPs against S. aureus and E. coli were 32 and 16 μg/mL, respectively. E. coli was more sensitive to AgNPs than S. aureus due to differences in cell wall structures of the bacteria. Regarding the bactericidal mechanisms of AgNPs, an increase in cell permeability and a distinctive deformation in cellular morphology was observed. The antibacterial effect decreased with the addition of the antioxidant N-acetyl-l-cysteine (NAC) which acted as ROS scavenger. In summary, the antibacterial mechanism was likely a combination of cell membrane damage and ROS induction.
以香蕉花提取物(BFE)为还原剂和稳定剂合成了纳米银。通过紫外-可见光谱(UV-vis)、透射电子显微镜(TEM)、x射线衍射(XRD)、傅里叶变换红外光谱(FT-IR)、热重分析(TGA)和zeta电位对制备的球形、分散良好、稳定的AgNPs进行了表征。研究了AgNPs对金黄色葡萄球菌和大肠杆菌的体外抑菌性能。AgNPs对金黄色葡萄球菌和大肠杆菌的最低抑制浓度(MIC)分别为32和16 μg/mL。由于大肠杆菌细胞壁结构的差异,大肠杆菌对AgNPs比金黄色葡萄球菌更敏感。关于AgNPs的杀菌机制,观察到细胞通透性增加和细胞形态的明显变形。随着抗氧化剂n -乙酰-l-半胱氨酸(NAC)的加入,抗菌效果下降,NAC具有清除ROS的作用。综上所述,抗菌机制可能是细胞膜损伤和ROS诱导的结合。
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引用次数: 0
Analysis of yeast diversity during spontaneous fermentation of red and white pitaya 红白火龙果自发发酵过程中酵母多样性分析
IF 0.8 4区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-06-21 DOI: 10.47836/ifrj.30.3.21
Xun Gong, Shiping Zou, Wénwén Liú, Zhihai Yu, Xiaozhu Liu
Yeast communities during spontaneous fermentation of red and white pitaya were characterised and compared to provide a reference for the isolation of pitaya yeast strains. Using Illumina MiSeq high-throughput sequencing technology, we investigated the composition and diversities of yeast communities during five stages of spontaneous fermentation (1, 3, 5, 7, and 15 d), and the results showed 584,055 and 699,120 valid sequences from red and white pitaya, respectively. The sequences were classified into 86 and 42 operational taxonomic units, then assigned to 69 species in 49 genera (red pitaya) and 37 species in 32 genera (white pitaya). Taxonomic composition and diversity analysis results showed high yeast diversity during the early stage of spontaneous fermentation (RF1) for red pitaya, and during the middle stage (WF5) for white pitaya fermentation. The dominant yeast species was unclassified_o_Saccharomycetales in red pitaya, and Clavispora opuntiae in white pitaya, and these species showed opposite trends during red and white pitaya fermentation. One-way ANOVA showed highly significant differences in unclassified_o_Saccharomycetales (p ≤ 0.01) in red pitaya, and in Clavispora opuntiae (p ≤ 0.001) in white pitaya across the five spontaneous fermentation stages. In addition, phylogenetic analysis revealed that unclassified_o_Saccharomycetales and Clavispora opuntiae were positioned farthest from other yeast species. Overall, yeast community diversity during spontaneous fermentation was higher in red rather than white pitaya.
对红白火龙果自发发酵过程中的酵母菌群落进行了表征和比较,为火龙果酵母菌的分离提供参考。利用Illumina MiSeq高通量测序技术,研究了火龙果自发发酵的5个阶段(1、3、5、7和15 d)酵母群落的组成和多样性,结果显示红火果和白火龙果分别有584,055和699,120个有效序列。将序列划分为86和42个操作分类单位,分别归属于红火龙果49属69种和白火龙果32属37种。分类组成和多样性分析结果表明,红火龙果自发发酵初期(RF1)和白火龙果自发发酵中期(WF5)酵母多样性较高。红火龙果的优势酵母菌种为未分类的o_saccharomycetales,白火龙果的优势酵母菌种为Clavispora opuntiae,在红白火龙果发酵过程中,优势酵母菌种呈相反趋势。单因素方差分析显示,红火龙果中unclassified_o_Saccharomycetales (p≤0.01)和白火龙果中Clavispora opuntiae (p≤0.001)在5个自然发酵阶段差异极显著。此外,系统发育分析显示unclassified_ocharomycetales和Clavispora opuntiae离其他酵母菌最远。总体而言,红火龙果自发发酵过程中酵母群落多样性高于白火龙果。
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引用次数: 0
期刊
international food research journal
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