Cancer cells differentiate themselves from normal cells in diminished expression of L-asparaginase. It is the enzyme that catalyzes the hydrolysis of L-Asparagine to L-aspartic and ammonia, because of these it is used as a medication and in food manufacturing. As a medication L-aspraginase is used to treat various types of leukemia such as, acute lymphoblastic leukemia, acute myeloma leukemia and non Hodgkin’s lymphoma. Hence they are not capable of producing L-asparaginase and mainly depend on the L-asparagine from circulating plasma pools. The clinical action of this enzyme is attributed to the reduction of L-asparaginase, since tumor cells unable to synthesis these amino acids are selectively killed by L-asparaginase depravation. This enzyme is widely distributed, being found in as it is widely distributed bacteria as well as found in animals, microbes and plant sources. In the present study L-asparaginase producing bacteria was isolated from Simarouba glauca . It was grown on the modified M9 medium in which L- asparagine was the major source of L- asparaginase production was detected by the formation of pink colored zones on the medium. After the partial purification of the L-Asparaginase enzyme, the enzyme activity was found to be 155.83 Units/ml and specific activity of 779.15. The optimum pH was to be found at pH 8 at a temperature of 37°C in the presence of 10mM Mg 2+ . The molecular identification was done by16S rDNA, PCR and sequence analysis by BLAST further confirmed that Bacillus cereus .
{"title":"Isolation and Characterization of L- Asparaginase Producing Endophytic Bacteria from Simarouba Gluaca","authors":"Pathuppilly Satheesan Kavya, Parakkottil Chothi Madhu","doi":"10.11648/j.ijast.20190301.11","DOIUrl":"https://doi.org/10.11648/j.ijast.20190301.11","url":null,"abstract":"Cancer cells differentiate themselves from normal cells in diminished expression of L-asparaginase. It is the enzyme that catalyzes the hydrolysis of L-Asparagine to L-aspartic and ammonia, because of these it is used as a medication and in food manufacturing. As a medication L-aspraginase is used to treat various types of leukemia such as, acute lymphoblastic leukemia, acute myeloma leukemia and non Hodgkin’s lymphoma. Hence they are not capable of producing L-asparaginase and mainly depend on the L-asparagine from circulating plasma pools. The clinical action of this enzyme is attributed to the reduction of L-asparaginase, since tumor cells unable to synthesis these amino acids are selectively killed by L-asparaginase depravation. This enzyme is widely distributed, being found in as it is widely distributed bacteria as well as found in animals, microbes and plant sources. In the present study L-asparaginase producing bacteria was isolated from Simarouba glauca . It was grown on the modified M9 medium in which L- asparagine was the major source of L- asparaginase production was detected by the formation of pink colored zones on the medium. After the partial purification of the L-Asparaginase enzyme, the enzyme activity was found to be 155.83 Units/ml and specific activity of 779.15. The optimum pH was to be found at pH 8 at a temperature of 37°C in the presence of 10mM Mg 2+ . The molecular identification was done by16S rDNA, PCR and sequence analysis by BLAST further confirmed that Bacillus cereus .","PeriodicalId":13766,"journal":{"name":"International Journal of Animal Science and Technology","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81974665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmad Sayid Abdullah, Edy . Susanto, Arif Aria Hertanto
Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan tahu dalam kosentrat dan hijauan terhadap performan kelinci jantan lokal. Penelitian ini dilaksanakan di Desa Pandanarang Kecamatan Kalitengah Kota Lamongan mulai tanggal 14 Mei 2015 sampai dengan tanggal 6 Juni 2015. Bahan yang digunakan adalah 18 ekor kelinci jantan lokal. Ransum yang digunakan adalah kangkung dan konsentrat dengan perbandingan 70:30. Perlakuan yang diberikan adalah P0 (70% dan 30% konsentrat kangkung) sebagai kontrol, P1 (pakan kontrol + 5% tepung ampas), P2 (pakan kontrol + 10% tahu). Parameter yang diukur adalah asupan pakan, pertambahan bobot badan (PBB), konversi pakan. Data dianalisis dengan analisis ragam berdasarkan pola searah Rancangan Acak (RAL). Dari hasil penelitian dapat disimpulkan bahwa penambahan tahu dalam ransum tidak (22,04)≥F tabel 1% (10,19) mempengaruhi kinerja kelinci lokal jantan.
{"title":"Pengaruh Penambahan Ampas Tahu dalam Penggunaan Konsentrat dan Hijauan terhadap Jumlah Konsumsi, PBB dan FCR Kelinci Lokal Jantan","authors":"Ahmad Sayid Abdullah, Edy . Susanto, Arif Aria Hertanto","doi":"10.30736/ijasc.v2i01.39","DOIUrl":"https://doi.org/10.30736/ijasc.v2i01.39","url":null,"abstract":"Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan tahu dalam kosentrat dan hijauan terhadap performan kelinci jantan lokal. Penelitian ini dilaksanakan di Desa Pandanarang Kecamatan Kalitengah Kota Lamongan mulai tanggal 14 Mei 2015 sampai dengan tanggal 6 Juni 2015. Bahan yang digunakan adalah 18 ekor kelinci jantan lokal. Ransum yang digunakan adalah kangkung dan konsentrat dengan perbandingan 70:30. Perlakuan yang diberikan adalah P0 (70% dan 30% konsentrat kangkung) sebagai kontrol, P1 (pakan kontrol + 5% tepung ampas), P2 (pakan kontrol + 10% tahu). Parameter yang diukur adalah asupan pakan, pertambahan bobot badan (PBB), konversi pakan. Data dianalisis dengan analisis ragam berdasarkan pola searah Rancangan Acak (RAL). Dari hasil penelitian dapat disimpulkan bahwa penambahan tahu dalam ransum tidak (22,04)≥F tabel 1% (10,19) mempengaruhi kinerja kelinci lokal jantan.","PeriodicalId":13766,"journal":{"name":"International Journal of Animal Science and Technology","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89178481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Penelitian ini dilakukan untuk mengetahui analisis pendapatan peternak ayam potong pejantan potong pejantan pada kemitraan dan mandiri di Kecamatan Paciran Kabupaten Lamongan. Penelitian ini dilaksanakan di Kabupaten Lamongan. Penelitian ini deskriptif yang bertujuan menggambarkan atau menguraikan variabel penelitian yang membandingkan pendapatan usaha peternakan Ayam potong pejantan yang pada kemitraan dan pada mandiri di Kecamatan Paciran Kabupaten Lamongan. Populasi dalam penelitian ini adalah enam orang yang pada pola kemitraan dan enam orang pola mandiri. Analisis data yang di gunakan pada penelitian ini adalah statistik deskriptif yaitu menghitung rata-rata biaya, penerimaan, pendapatan rata-rata. Selanjutnya di analisis Statistik menggunakan uji T dengan hasil T hitung 114,33 > T tabel 3,059 (1%) 2,179 (5%) yang berarti menunjukkan bahwa perlakuan berbeda sangat nyata (P>5%). Kesimpulan dari penelitian ini adalah pendapatan peternak yang pada kemitraan cenderung lebih tinggi di bandingkan pendapatan peternak yang mandiri.
{"title":"Analisis Pendapatan Peternak Ayam Potong Pejantan pada Mandiri dan Kemitraan di Kecamatan Paciran Kabupaten Lamongan","authors":"S. Shohib, M. Dahlan, W. Wardoyo","doi":"10.30736/ijasc.v2i01.40","DOIUrl":"https://doi.org/10.30736/ijasc.v2i01.40","url":null,"abstract":"Penelitian ini dilakukan untuk mengetahui analisis pendapatan peternak ayam potong pejantan potong pejantan pada kemitraan dan mandiri di Kecamatan Paciran Kabupaten Lamongan. Penelitian ini dilaksanakan di Kabupaten Lamongan. Penelitian ini deskriptif yang bertujuan menggambarkan atau menguraikan variabel penelitian yang membandingkan pendapatan usaha peternakan Ayam potong pejantan yang pada kemitraan dan pada mandiri di Kecamatan Paciran Kabupaten Lamongan. Populasi dalam penelitian ini adalah enam orang yang pada pola kemitraan dan enam orang pola mandiri. Analisis data yang di gunakan pada penelitian ini adalah statistik deskriptif yaitu menghitung rata-rata biaya, penerimaan, pendapatan rata-rata. Selanjutnya di analisis Statistik menggunakan uji T dengan hasil T hitung 114,33 > T tabel 3,059 (1%) 2,179 (5%) yang berarti menunjukkan bahwa perlakuan berbeda sangat nyata (P>5%). Kesimpulan dari penelitian ini adalah pendapatan peternak yang pada kemitraan cenderung lebih tinggi di bandingkan pendapatan peternak yang mandiri.","PeriodicalId":13766,"journal":{"name":"International Journal of Animal Science and Technology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73621370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.11648/j.ijast.20190301.12
Kassa Sagui Kévin
{"title":"Genetic Diversity of Benin Cattle Populations Using Microsatellite Markers","authors":"Kassa Sagui Kévin","doi":"10.11648/j.ijast.20190301.12","DOIUrl":"https://doi.org/10.11648/j.ijast.20190301.12","url":null,"abstract":"","PeriodicalId":13766,"journal":{"name":"International Journal of Animal Science and Technology","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78155531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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